AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0467150

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0467150 MtrunA17_Chr3g0121251 98.387 248 3 1 1 247 1 248 3.45e-166 459
Msa0467150 MtrunA17_Chr4g0066591 61.538 91 35 0 38 128 46 136 2.06e-34 122
Msa0467150 MtrunA17_Chr1g0176111 64.348 115 19 4 79 187 2 100 3.89e-33 117
Msa0467150 MtrunA17_Chr1g0176121 80.488 41 8 0 38 78 41 81 5.48e-18 76.6
Msa0467150 MtrunA17_Chr5g0435241 62.745 51 19 0 79 129 10 60 6.05e-16 74.3
Msa0467150 MtrunA17_Chr4g0050441 41.558 77 45 0 41 117 17 93 6.49e-13 67.0
Msa0467150 MtrunA17_Chr5g0435231 62.791 43 16 0 41 83 48 90 1.27e-12 63.2
Msa0467150 MtrunA17_Chr5g0429941 40.260 77 46 0 41 117 17 93 4.37e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0467150 AT3G13570.1 62.651 249 74 8 1 247 1 232 1.64e-76 232
Msa0467150 AT1G55310.5 62.771 231 71 5 1 231 1 216 1.02e-71 219
Msa0467150 AT1G55310.2 62.771 231 71 5 1 231 1 216 1.02e-71 219
Msa0467150 AT1G55310.1 63.677 223 66 5 1 223 1 208 1.97e-69 215
Msa0467150 AT1G55310.3 60.426 235 66 6 1 223 1 220 4.00e-66 207
Msa0467150 AT5G18810.2 57.407 108 46 0 35 142 42 149 5.20e-37 130
Msa0467150 AT5G18810.1 57.407 108 46 0 35 142 42 149 5.33e-37 130
Msa0467150 AT3G55460.1 57.895 95 40 0 42 136 49 143 3.38e-34 124
Msa0467150 AT1G55310.6 55.882 136 48 5 89 223 1 125 1.25e-27 105
Msa0467150 AT1G55310.7 55.882 136 48 5 89 223 1 125 1.25e-27 105
Msa0467150 AT1G55310.4 56.296 135 48 4 89 223 1 124 1.36e-21 89.4
Msa0467150 AT5G64200.2 39.130 92 54 1 41 130 17 108 1.79e-13 68.9
Msa0467150 AT5G64200.1 39.130 92 54 1 41 130 17 108 1.79e-13 68.9

Find 108 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTAGACCTTTCGGTCATTT+TGG 0.233664 3_3:-68381823 Msa0467150:CDS
GTTCAGCAAAACAATGGATT+TGG 0.245219 3_3:-68379550 Msa0467150:three_prime_UTR
GCTTCTCACATCCCCGTTCT+TGG 0.247700 3_3:+68379718 None:intergenic
TGAGACCGAAGGAGTTATTT+TGG 0.251450 3_3:+68379351 None:intergenic
CTCGTTCACCCCGCTATTCC+CGG 0.259956 3_3:-68380164 Msa0467150:CDS
TAACCCAGTGTAATAATCTT+TGG 0.277366 3_3:+68381777 None:intergenic
TACAGGCAACCTCGTGGTTT+TGG 0.283342 3_3:-68381342 Msa0467150:intron
TAGATTTAGTAGAATTATAT+TGG 0.296374 3_3:-68379428 Msa0467150:three_prime_UTR
CTTGAATATTCCCTTCGTTT+AGG 0.306626 3_3:+68380084 None:intergenic
AACCCAGTGTAATAATCTTT+GGG 0.338449 3_3:+68381778 None:intergenic
ATGGATGGTCAAGTTCTTCT+TGG 0.350938 3_3:-68381270 Msa0467150:CDS
TCTCTTCCATTGTCACTGAC+TGG 0.377083 3_3:+68379607 None:intergenic
CACGCTCTCCACCCAAGAAC+GGG 0.377947 3_3:-68379730 Msa0467150:CDS
CAAGTCCGAGCCGTGATGTC+AGG 0.383176 3_3:-68379652 Msa0467150:CDS
AAAGGTCTACGGAGATCTTC+AGG 0.385331 3_3:+68381835 None:intergenic
CTTCTCACATCCCCGTTCTT+GGG 0.393169 3_3:+68379719 None:intergenic
GAAGATCTCCGTAGACCTTT+CGG 0.405470 3_3:-68381832 Msa0467150:CDS
ATAATTCTACTAAATCTAGA+TGG 0.405851 3_3:+68379433 None:intergenic
CTGTATCACCTGAGGATAGA+AGG 0.408964 3_3:-68379796 Msa0467150:CDS
ACCAAGCATCTATTGTCTAA+TGG 0.413297 3_3:+68379583 None:intergenic
AGGTGGAGACCGGGAATAGC+GGG 0.413550 3_3:+68380155 None:intergenic
ATCTGTGACGAGGTGGAGAC+CGG 0.419316 3_3:+68380145 None:intergenic
TACAGCAGAAGAGGCGGCGG+AGG 0.422786 3_3:-68381952 Msa0467150:CDS
AGCAGAAGAGGCGGCGGAGG+AGG 0.424350 3_3:-68381949 Msa0467150:CDS
CTCTCGACTGAGCCTTTCAC+TGG 0.429082 3_3:+68379683 None:intergenic
GGGAGATAAATATCCTTAAG+AGG 0.431391 3_3:+68381799 None:intergenic
CTCTTCCATTGTCACTGACT+GGG 0.434177 3_3:+68379608 None:intergenic
CTGACATCACGGCTCGGACT+TGG 0.436240 3_3:+68379653 None:intergenic
CTTTCACTGGACTCTGACTA+CGG 0.452235 3_3:+68379696 None:intergenic
TTAGTAGAATTATATTGGTA+TGG 0.452275 3_3:-68379423 Msa0467150:three_prime_UTR
CTAAACGAAGGGAATATTCA+AGG 0.464257 3_3:-68380083 Msa0467150:intron
CTCCCCAAAGATTATTACAC+TGG 0.473935 3_3:-68381781 Msa0467150:CDS
ACCATTAGACAATAGATGCT+TGG 0.482289 3_3:-68379584 Msa0467150:three_prime_UTR
CTGTAACGCGGTGGTGGAGA+TGG 0.491469 3_3:+68381970 None:intergenic
TGGAGCCAAAATAACTCCTT+CGG 0.492312 3_3:-68379356 Msa0467150:three_prime_UTR
CTTCTGCTGTAACGCGGTGG+TGG 0.495589 3_3:+68381964 None:intergenic
GGTAGGTAGATCGCTTTGAC+GGG 0.497430 3_3:+68381894 None:intergenic
GCGTAACGCCGGCGAGGACT+TGG 0.497603 3_3:+68381919 None:intergenic
TCTCTGACCCTCATCTCAGT+TGG 0.506671 3_3:+68381204 None:intergenic
TTCCCTTCGTTTAGGAGGAG+GGG 0.509465 3_3:+68380092 None:intergenic
TTGAATGTTCAGCAAAACAA+TGG 0.519628 3_3:-68379556 Msa0467150:three_prime_UTR
TGGTAGGTAGATCGCTTTGA+CGG 0.523524 3_3:+68381893 None:intergenic
ATGGTCAAGTTCTTCTTGGT+AGG 0.524497 3_3:-68381266 Msa0467150:CDS
TATTCCCTTCGTTTAGGAGG+AGG 0.525431 3_3:+68380090 None:intergenic
TGGTCAAGTTCTTCTTGGTA+GGG 0.533607 3_3:-68381265 Msa0467150:CDS
CGACTGTGCCTTCTATCCTC+AGG 0.535468 3_3:+68379788 None:intergenic
CTCACATCCCCGTTCTTGGG+TGG 0.544494 3_3:+68379722 None:intergenic
ATTCCCTTCGTTTAGGAGGA+GGG 0.548091 3_3:+68380091 None:intergenic
TCTGTGACGAGGTGGAGACC+GGG 0.551287 3_3:+68380146 None:intergenic
GATAGAAGGCACAGTCGAGA+AGG 0.551688 3_3:-68379782 Msa0467150:CDS
CCACCGCGTTACAGCAGAAG+AGG 0.552873 3_3:-68381961 Msa0467150:CDS
TTCGCATCAGCAGCATCCGC+AGG 0.554382 3_3:+68381300 None:intergenic
CAAAATGACCGAAAGGTCTA+CGG 0.557171 3_3:+68381824 None:intergenic
CATGGCGAAGGTTACGAACA+AGG 0.560608 3_3:+68381866 None:intergenic
AAGTCCGAGCCGTGATGTCA+GGG 0.561984 3_3:-68379651 Msa0467150:CDS
GAGGTGGAGACCGGGAATAG+CGG 0.564550 3_3:+68380154 None:intergenic
GAATATTCCCTTCGTTTAGG+AGG 0.564905 3_3:+68380087 None:intergenic
GATCTTCAGGCCTACAGTCA+TGG 0.565387 3_3:+68381848 None:intergenic
CGTAGTCAGAGTCCAGTGAA+AGG 0.572092 3_3:-68379695 Msa0467150:CDS
GGTCTCCCAGTCAGTGACAA+TGG 0.573445 3_3:-68379613 Msa0467150:exon
TCACGCTCTCCACCCAAGAA+CGG 0.579197 3_3:-68379731 Msa0467150:CDS
TGCTGATGCGAAATATCATA+TGG 0.579685 3_3:-68381289 Msa0467150:CDS
GAAAGAAGCCAACTGAGATG+AGG 0.582129 3_3:-68381212 Msa0467150:CDS
GTAACCTTCGCCATGACTGT+AGG 0.586128 3_3:-68381858 Msa0467150:CDS
GAAGAAGTCCAAGTCCTCGC+CGG 0.586465 3_3:-68381927 Msa0467150:CDS
TCAGGGAAGCATCCCGCAGT+AGG 0.587173 3_3:-68379634 Msa0467150:CDS
GCTTCCCTGACATCACGGCT+CGG 0.592080 3_3:+68379647 None:intergenic
TTTGACGGGGAGCGTAACGC+CGG 0.593260 3_3:+68381908 None:intergenic
TTCCCCTCCTCCTAAACGAA+GGG 0.597370 3_3:-68380094 Msa0467150:CDS
TCCCCAAAGATTATTACACT+GGG 0.598302 3_3:-68381780 Msa0467150:intron
AAAGAAGCCAACTGAGATGA+GGG 0.598374 3_3:-68381211 Msa0467150:CDS
AAGGTTACGAACAAGGAGAC+TGG 0.604315 3_3:+68381873 None:intergenic
CGGGGTGAACGAGAATAACG+TGG 0.606363 3_3:+68380174 None:intergenic
GTTTATGTTTCAGTAGCGGT+CGG 0.608429 3_3:-68380221 Msa0467150:intron
TTGTAGATCTGTATCACCTG+AGG 0.616753 3_3:-68379804 Msa0467150:intron
TATTCAATTTGTGGATCCTG+CGG 0.619040 3_3:-68381316 Msa0467150:CDS
CAAAGCAAAGGATCACTCGC+CGG 0.620800 3_3:-68382029 Msa0467150:five_prime_UTR
CCTCTTCTGCTGTAACGCGG+TGG 0.622436 3_3:+68381961 None:intergenic
AGATGAGGGTCAGAGAGAGA+AGG 0.623453 3_3:-68381197 Msa0467150:intron
ATTCCCCTCCTCCTAAACGA+AGG 0.624660 3_3:-68380095 Msa0467150:CDS
CGTTGTCATCTGAAGATGAG+AGG 0.624703 3_3:-68382006 Msa0467150:exon
CGTTACAGCAGAAGAGGCGG+CGG 0.624756 3_3:-68381955 Msa0467150:CDS
GGGATGCTTCCCTGACATCA+CGG 0.627142 3_3:+68379642 None:intergenic
CCGCCTCTTCTGCTGTAACG+CGG 0.629314 3_3:+68381958 None:intergenic
ACCCAGTGTAATAATCTTTG+GGG 0.631656 3_3:+68381779 None:intergenic
GGTGGAGACCGGGAATAGCG+GGG 0.633611 3_3:+68380156 None:intergenic
GTCGGCATTCTGATCGAAGG+AGG 0.634775 3_3:-68380203 Msa0467150:CDS
GAGCGAGATCTGTGACGAGG+TGG 0.637564 3_3:+68380138 None:intergenic
CAGGCCTACAGTCATGGCGA+AGG 0.637832 3_3:+68381854 None:intergenic
GCGGTCGGCATTCTGATCGA+AGG 0.640639 3_3:-68380206 Msa0467150:CDS
GATGCGAAATATCATATGGA+TGG 0.642241 3_3:-68381285 Msa0467150:CDS
AGAGGACCAAAATGACCGAA+AGG 0.644554 3_3:+68381817 None:intergenic
CTGACTGGGAGACCTACTGC+GGG 0.645256 3_3:+68379622 None:intergenic
GATCACAACACAGCAGAGAG+AGG 0.645731 3_3:-68379760 Msa0467150:CDS
CCGCGTTACAGCAGAAGAGG+CGG 0.649844 3_3:-68381958 Msa0467150:CDS
CGGGGAGCGTAACGCCGGCG+AGG 0.649965 3_3:+68381913 None:intergenic
TTACGAACAAGGAGACTGGT+AGG 0.662391 3_3:+68381877 None:intergenic
ACGCTCTCCACCCAAGAACG+GGG 0.664146 3_3:-68379729 Msa0467150:CDS
CGTCACAGATCTCGCTCTCG+CGG 0.666167 3_3:-68380132 Msa0467150:CDS
GGGGTGAACGAGAATAACGT+GGG 0.666188 3_3:+68380175 None:intergenic
CGAGAGCGAGATCTGTGACG+AGG 0.668387 3_3:+68380135 None:intergenic
ACTGACTGGGAGACCTACTG+CGG 0.671960 3_3:+68379621 None:intergenic
GCTTTCTACAGGCAACCTCG+TGG 0.675676 3_3:-68381348 Msa0467150:intron
GTAGGTAGATCGCTTTGACG+GGG 0.676212 3_3:+68381895 None:intergenic
TCATCTTCAGATGACAACGC+CGG 0.731677 3_3:+68382010 None:intergenic
GGGTGAACGAGAATAACGTG+GGG 0.732637 3_3:+68380176 None:intergenic
GGTGAACGAGAATAACGTGG+GGG 0.773739 3_3:+68380177 None:intergenic
TGAATAAAACCAAAACCACG+AGG 0.777977 3_3:+68381333 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTATTTTTTTTAATTATA+AGG - chr3_3:68381551-68381570 Msa0467150:intron 0.0%
!! TTTGTATTTACTTAAAATAA+TGG - chr3_3:68381383-68381402 Msa0467150:intron 10.0%
!!! AAAATTTAGGGTTATATTAT+TGG - chr3_3:68379712-68379731 Msa0467150:CDS 15.0%
!!! AAATTTAGGGTTATATTATT+GGG - chr3_3:68379713-68379732 Msa0467150:CDS 15.0%
!! TTTAATAATTGTGAGAAATT+AGG - chr3_3:68379744-68379763 Msa0467150:CDS 15.0%
!!! TTGATTTGATCTTATATTAT+TGG - chr3_3:68379913-68379932 Msa0467150:intron 15.0%
!! TGATTTGATCTTATATTATT+GGG - chr3_3:68379914-68379933 Msa0467150:intron 15.0%
!! GTAAATGATGATAATAAATA+AGG + chr3_3:68381050-68381069 None:intergenic 15.0%
!! TAGATTTAGTAGAATTATAT+TGG - chr3_3:68381970-68381989 Msa0467150:CDS 15.0%
!! ATTGAATGCTTTCAAAATTT+AGG - chr3_3:68379699-68379718 Msa0467150:CDS 20.0%
!! TTGAATGCTTTCAAAATTTA+GGG - chr3_3:68379700-68379719 Msa0467150:CDS 20.0%
!!! AATTTAGGGTTATATTATTG+GGG - chr3_3:68379714-68379733 Msa0467150:CDS 20.0%
!!! TTTTGGTTTTATTCAATTTG+TGG - chr3_3:68380073-68380092 Msa0467150:intron 20.0%
!! ATTATTGTTGAAATTCAAAG+TGG + chr3_3:68380373-68380392 None:intergenic 20.0%
!! GATTGTTTATATGCAAATAA+GGG + chr3_3:68380810-68380829 None:intergenic 20.0%
!! AACAACAATCATTTAAACTT+TGG + chr3_3:68381025-68381044 None:intergenic 20.0%
!! AACAGTATACTTATAAGTAT+TGG + chr3_3:68381072-68381091 None:intergenic 20.0%
!! ATAATTCTACTAAATCTAGA+TGG + chr3_3:68381968-68381987 None:intergenic 20.0%
!! TTAGTAGAATTATATTGGTA+TGG - chr3_3:68381975-68381994 Msa0467150:CDS 20.0%
! AAAAATTAGAAGCAAAGCAA+AGG - chr3_3:68379357-68379376 Msa0467150:three_prime_UTR 25.0%
! TCATCTTAATTTCACACAAA+AGG - chr3_3:68379863-68379882 Msa0467150:intron 25.0%
! AATTGAAAAAGAAACGAAGA+GGG + chr3_3:68380235-68380254 None:intergenic 25.0%
! AAATTGAAAAAGAAACGAAG+AGG + chr3_3:68380236-68380255 None:intergenic 25.0%
!!! CTTCGTTTCTTTTTCAATTT+TGG - chr3_3:68380236-68380255 Msa0467150:intron 25.0%
! TGACTTTGAATGAATCAATA+TGG - chr3_3:68380262-68380281 Msa0467150:intron 25.0%
! GACTTTGAATGAATCAATAT+GGG - chr3_3:68380263-68380282 Msa0467150:intron 25.0%
!!! GGATTATAAGATTAGTTTAG+TGG - chr3_3:68380329-68380348 Msa0467150:intron 25.0%
! GAATTTCAACAATAATTGTG+TGG - chr3_3:68380377-68380396 Msa0467150:intron 25.0%
!! TGTCTTTTATTGCGTTATAA+TGG - chr3_3:68380456-68380475 Msa0467150:intron 25.0%
! ATAGATTGGTAGATTTCATT+GGG + chr3_3:68380599-68380618 None:intergenic 25.0%
! AATAGATTGGTAGATTTCAT+TGG + chr3_3:68380600-68380619 None:intergenic 25.0%
! CTGTTTATATGCAAATAGAT+TGG + chr3_3:68380613-68380632 None:intergenic 25.0%
! ATCTATTTGCATATAAACAG+AGG - chr3_3:68380613-68380632 Msa0467150:intron 25.0%
! TCTATTTGCATATAAACAGA+GGG - chr3_3:68380614-68380633 Msa0467150:intron 25.0%
! GGATTGTTTATATGCAAATA+AGG + chr3_3:68380811-68380830 None:intergenic 25.0%
! ACATTTCAAACAACAAATTC+AGG + chr3_3:68380884-68380903 None:intergenic 25.0%
!! TGTTGTTTGAAATGTAATGA+AGG - chr3_3:68380889-68380908 Msa0467150:intron 25.0%
! AATTTACTTGCAGTAATCAA+TGG + chr3_3:68380937-68380956 None:intergenic 25.0%
! TAATGTTTATGTTTCAGTAG+CGG - chr3_3:68381173-68381192 Msa0467150:intron 25.0%
! AATTATTATTAAGTGACCAC+TGG + chr3_3:68381347-68381366 None:intergenic 25.0%
! TATAAATGTTTAGAAAGAGC+TGG - chr3_3:68381408-68381427 Msa0467150:intron 25.0%
!! TATATTGGTATGGATTTTTC+TGG - chr3_3:68381985-68382004 Msa0467150:CDS 25.0%
!! TTTTTGTCTTGTCTTCATTA+TGG - chr3_3:68382022-68382041 Msa0467150:five_prime_UTR 25.0%
AACCCAGTGTAATAATCTTT+GGG + chr3_3:68379623-68379642 None:intergenic 30.0%
TAACCCAGTGTAATAATCTT+TGG + chr3_3:68379624-68379643 None:intergenic 30.0%
ATGAAATCTTCTTCAAGACT+TGG + chr3_3:68379847-68379866 None:intergenic 30.0%
!! TAAAATTGGTGCTAAGAAGT+TGG + chr3_3:68379949-68379968 None:intergenic 30.0%
! CAATTTTACTCACTGCATAT+GGG - chr3_3:68379961-68379980 Msa0467150:intron 30.0%
!!! TGTGTTAATGCTTTTTTCGT+TGG - chr3_3:68380010-68380029 Msa0467150:intron 30.0%
!!! GTGTTAATGCTTTTTTCGTT+GGG - chr3_3:68380011-68380030 Msa0467150:intron 30.0%
!! GTTGGGTTTTCTGTTTTATT+CGG - chr3_3:68380028-68380047 Msa0467150:intron 30.0%
CTATGTGTATTTACAGGAAA+CGG + chr3_3:68380305-68380324 None:intergenic 30.0%
AATTGTGTGGTATACTAGTA+AGG - chr3_3:68380390-68380409 Msa0467150:intron 30.0%
TTATTGCGTTATAATGGTAC+AGG - chr3_3:68380462-68380481 Msa0467150:intron 30.0%
TAGATTGGTAGATTTCATTG+GGG + chr3_3:68380598-68380617 None:intergenic 30.0%
CTATTTGCATATAAACAGAG+GGG - chr3_3:68380615-68380634 Msa0467150:intron 30.0%
TATAATAACATCGATGAGCT+CGG - chr3_3:68380858-68380877 Msa0467150:intron 30.0%
GCTAAATTTCACTGGAAAAA+AGG + chr3_3:68380915-68380934 None:intergenic 30.0%
AATCAATGGCTAAATTTCAC+TGG + chr3_3:68380923-68380942 None:intergenic 30.0%
TACTGCAAGTAAATTACACT+AGG - chr3_3:68380942-68380961 Msa0467150:intron 30.0%
GATTATGTATGTGTTTGTTC+CGG - chr3_3:68381500-68381519 Msa0467150:intron 30.0%
TTGAATGTTCAGCAAAACAA+TGG - chr3_3:68381842-68381861 Msa0467150:CDS 30.0%
!!! AAAAATGTTTTAGTGCTAGC+TGG - chr3_3:68381914-68381933 Msa0467150:CDS 30.0%
GGGAGATAAATATCCTTAAG+AGG + chr3_3:68379602-68379621 None:intergenic 35.0%
TCCCCAAAGATTATTACACT+GGG - chr3_3:68379618-68379637 Msa0467150:exon 35.0%
ACCCAGTGTAATAATCTTTG+GGG + chr3_3:68379622-68379641 None:intergenic 35.0%
CCATATGCAGTGAGTAAAAT+TGG + chr3_3:68379963-68379982 None:intergenic 35.0%
! CCAATTTTACTCACTGCATA+TGG - chr3_3:68379960-68379979 Msa0467150:intron 35.0%
!! TGTTTTATTCGGCTTTCTAC+AGG - chr3_3:68380039-68380058 Msa0467150:intron 35.0%
TGAATAAAACCAAAACCACG+AGG + chr3_3:68380068-68380087 None:intergenic 35.0%
TATTCAATTTGTGGATCCTG+CGG - chr3_3:68380082-68380101 Msa0467150:intron 35.0%
TGCTGATGCGAAATATCATA+TGG - chr3_3:68380109-68380128 Msa0467150:CDS 35.0%
GATGCGAAATATCATATGGA+TGG - chr3_3:68380113-68380132 Msa0467150:CDS 35.0%
!!! GATAACTGTTGTTTTTGCTG+AGG - chr3_3:68380157-68380176 Msa0467150:CDS 35.0%
CAATATGGGATACTTGCATT+TGG - chr3_3:68380277-68380296 Msa0467150:intron 35.0%
CCTAGACTATGTGTATTTAC+AGG + chr3_3:68380311-68380330 None:intergenic 35.0%
CCTGTAAATACACATAGTCT+AGG - chr3_3:68380308-68380327 Msa0467150:intron 35.0%
! ATACTAGTAAGGCCCTATTT+TGG - chr3_3:68380401-68380420 Msa0467150:intron 35.0%
TATAAACAGCCTGAAATCCA+AGG + chr3_3:68380499-68380518 None:intergenic 35.0%
TTAATAAGTTCCGTGATCGA+TGG + chr3_3:68380535-68380554 None:intergenic 35.0%
AGATTGGTAGATTTCATTGG+GGG + chr3_3:68380597-68380616 None:intergenic 35.0%
!!! TTTCCCTGTTTTTACACAAG+AGG + chr3_3:68380711-68380730 None:intergenic 35.0%
TCTTGTGTAAAAACAGGGAA+AGG - chr3_3:68380710-68380729 Msa0467150:intron 35.0%
TCATAGATTCAAACCGAGAT+CGG + chr3_3:68380836-68380855 None:intergenic 35.0%
AATTACACTAGGCCAGTTTA+TGG - chr3_3:68380953-68380972 Msa0467150:intron 35.0%
ATTACACTAGGCCAGTTTAT+GGG - chr3_3:68380954-68380973 Msa0467150:intron 35.0%
ACGAGACATTAAAATGTCCT+GGG - chr3_3:68380993-68381012 Msa0467150:intron 35.0%
TTAAACTTTGGATGAAACCC+AGG + chr3_3:68381013-68381032 None:intergenic 35.0%
CTTGAATATTCCCTTCGTTT+AGG + chr3_3:68381317-68381336 None:intergenic 35.0%
CTAAACGAAGGGAATATTCA+AGG - chr3_3:68381315-68381334 Msa0467150:CDS 35.0%
GAAGGGAATATTCAAGGTAT+TGG - chr3_3:68381321-68381340 Msa0467150:CDS 35.0%
!! ACATACTGTTGGTTCTAGTT+TGG - chr3_3:68381437-68381456 Msa0467150:intron 35.0%
!! CATACTGTTGGTTCTAGTTT+GGG - chr3_3:68381438-68381457 Msa0467150:intron 35.0%
GTGATACAGATCTACAAAAC+AGG + chr3_3:68381591-68381610 None:intergenic 35.0%
ACCATTAGACAATAGATGCT+TGG - chr3_3:68381814-68381833 Msa0467150:CDS 35.0%
ACCAAGCATCTATTGTCTAA+TGG + chr3_3:68381818-68381837 None:intergenic 35.0%
! GTTCAGCAAAACAATGGATT+TGG - chr3_3:68381848-68381867 Msa0467150:CDS 35.0%
CAAAATGACCGAAAGGTCTA+CGG + chr3_3:68379577-68379596 None:intergenic 40.0%
CTCCCCAAAGATTATTACAC+TGG - chr3_3:68379617-68379636 Msa0467150:exon 40.0%
! TATGGGTCTCGTTTCGATTT+TGG - chr3_3:68379978-68379997 Msa0467150:intron 40.0%
ATGGATGGTCAAGTTCTTCT+TGG - chr3_3:68380128-68380147 Msa0467150:CDS 40.0%
ATGGTCAAGTTCTTCTTGGT+AGG - chr3_3:68380132-68380151 Msa0467150:CDS 40.0%
TGGTCAAGTTCTTCTTGGTA+GGG - chr3_3:68380133-68380152 Msa0467150:CDS 40.0%
AAAGAAGCCAACTGAGATGA+GGG - chr3_3:68380187-68380206 Msa0467150:CDS 40.0%
TGCGTTATAATGGTACAGGT+AGG - chr3_3:68380466-68380485 Msa0467150:intron 40.0%
GCGTTATAATGGTACAGGTA+GGG - chr3_3:68380467-68380486 Msa0467150:intron 40.0%
ACTTAAACCACCATCGATCA+CGG - chr3_3:68380522-68380541 Msa0467150:intron 40.0%
GATTGGTAGATTTCATTGGG+GGG + chr3_3:68380596-68380615 None:intergenic 40.0%
TATAAACAGAGGGGTAACCT+TGG - chr3_3:68380624-68380643 Msa0467150:intron 40.0%
GTTGTTGTCATGTGACTGTA+AGG - chr3_3:68380660-68380679 Msa0467150:intron 40.0%
ACAGCCTCTTGTGTAAAAAC+AGG - chr3_3:68380704-68380723 Msa0467150:intron 40.0%
CAGCCTCTTGTGTAAAAACA+GGG - chr3_3:68380705-68380724 Msa0467150:intron 40.0%
CGTACAATACACCAAATGGT+GGG - chr3_3:68380736-68380755 Msa0467150:intron 40.0%
ATATGCAAATAAGGGCAACC+CGG + chr3_3:68380802-68380821 None:intergenic 40.0%
CAATGACAAGACCCATAAAC+TGG + chr3_3:68380968-68380987 None:intergenic 40.0%
GACGAGACATTAAAATGTCC+TGG - chr3_3:68380992-68381011 Msa0467150:intron 40.0%
TTCTGCTTGTTTCAGCTAAG+AGG - chr3_3:68381123-68381142 Msa0467150:intron 40.0%
GTTTATGTTTCAGTAGCGGT+CGG - chr3_3:68381177-68381196 Msa0467150:intron 40.0%
GAATATTCCCTTCGTTTAGG+AGG + chr3_3:68381314-68381333 None:intergenic 40.0%
!! ATATTCAAGGTATTGGCCAG+TGG - chr3_3:68381328-68381347 Msa0467150:CDS 40.0%
TTGTTCCGGTCAAAAGATGT+TGG - chr3_3:68381514-68381533 Msa0467150:intron 40.0%
TTGTAGATCTGTATCACCTG+AGG - chr3_3:68381594-68381613 Msa0467150:intron 40.0%
TGGAGCCAAAATAACTCCTT+CGG - chr3_3:68382042-68382061 Msa0467150:five_prime_UTR 40.0%
! TGAGACCGAAGGAGTTATTT+TGG + chr3_3:68382050-68382069 None:intergenic 40.0%
AAAAAAGGTCATGAGACCGA+AGG + chr3_3:68382061-68382080 None:intergenic 40.0%
TCATCTTCAGATGACAACGC+CGG + chr3_3:68379391-68379410 None:intergenic 45.0%
CGTTGTCATCTGAAGATGAG+AGG - chr3_3:68379392-68379411 Msa0467150:three_prime_UTR 45.0%
TGGTAGGTAGATCGCTTTGA+CGG + chr3_3:68379508-68379527 None:intergenic 45.0%
TTACGAACAAGGAGACTGGT+AGG + chr3_3:68379524-68379543 None:intergenic 45.0%
AAGGTTACGAACAAGGAGAC+TGG + chr3_3:68379528-68379547 None:intergenic 45.0%
AAAGGTCTACGGAGATCTTC+AGG + chr3_3:68379566-68379585 None:intergenic 45.0%
GAAGATCTCCGTAGACCTTT+CGG - chr3_3:68379566-68379585 Msa0467150:three_prime_UTR 45.0%
! CGTAGACCTTTCGGTCATTT+TGG - chr3_3:68379575-68379594 Msa0467150:three_prime_UTR 45.0%
AGAGGACCAAAATGACCGAA+AGG + chr3_3:68379584-68379603 None:intergenic 45.0%
!! CGGTCATTTTGGTCCTCTTA+AGG - chr3_3:68379586-68379605 Msa0467150:three_prime_UTR 45.0%
GAAAGAAGCCAACTGAGATG+AGG - chr3_3:68380186-68380205 Msa0467150:CDS 45.0%
!!! TAAGGCCCTATTTTGGTCCT+CGG - chr3_3:68380408-68380427 Msa0467150:intron 45.0%
CTCTTCCGAGGACCAAAATA+GGG + chr3_3:68380416-68380435 None:intergenic 45.0%
TCTCTTCCGAGGACCAAAAT+AGG + chr3_3:68380417-68380436 None:intergenic 45.0%
!! ATTTTGGTCCTCGGAAGAGA+TGG - chr3_3:68380417-68380436 Msa0467150:intron 45.0%
! TTTTGGTCCTCGGAAGAGAT+GGG - chr3_3:68380418-68380437 Msa0467150:intron 45.0%
TACAGGTAGGGTTCAAACCT+TGG - chr3_3:68380479-68380498 Msa0467150:intron 45.0%
GGGTTCAAACCTTGGATTTC+AGG - chr3_3:68380487-68380506 Msa0467150:intron 45.0%
ATAAGTTCCGTGATCGATGG+TGG + chr3_3:68380532-68380551 None:intergenic 45.0%
GTCATGTGACTGTAAGGTCA+CGG - chr3_3:68380666-68380685 Msa0467150:intron 45.0%
TCATGTGACTGTAAGGTCAC+GGG - chr3_3:68380667-68380686 Msa0467150:intron 45.0%
! TTACACAAGAGGCTGTTTCC+AGG + chr3_3:68380700-68380719 None:intergenic 45.0%
GCTGCGTACAATACACCAAA+TGG - chr3_3:68380732-68380751 Msa0467150:intron 45.0%
GCGTACAATACACCAAATGG+TGG - chr3_3:68380735-68380754 Msa0467150:intron 45.0%
ATAAACAATCCCGCCGATCT+CGG - chr3_3:68380820-68380839 Msa0467150:intron 45.0%
TAGATTCAAACCGAGATCGG+CGG + chr3_3:68380833-68380852 None:intergenic 45.0%
CTTGTTTCAGCTAAGAGGTG+AGG - chr3_3:68381128-68381147 Msa0467150:intron 45.0%
ATTCCCTTCGTTTAGGAGGA+GGG + chr3_3:68381310-68381329 None:intergenic 45.0%
TATTCCCTTCGTTTAGGAGG+AGG + chr3_3:68381311-68381330 None:intergenic 45.0%
TTCCGGTCAAAAGATGTTGG+TGG - chr3_3:68381517-68381536 Msa0467150:intron 45.0%
! CTCCACCAACATCTTTTGAC+CGG + chr3_3:68381522-68381541 None:intergenic 45.0%
CTGTATCACCTGAGGATAGA+AGG - chr3_3:68381602-68381621 Msa0467150:intron 45.0%
CTTTCACTGGACTCTGACTA+CGG + chr3_3:68381705-68381724 None:intergenic 45.0%
! CTCTTCCATTGTCACTGACT+GGG + chr3_3:68381793-68381812 None:intergenic 45.0%
TCTCTTCCATTGTCACTGAC+TGG + chr3_3:68381794-68381813 None:intergenic 45.0%
CAAAGCAAAGGATCACTCGC+CGG - chr3_3:68379369-68379388 Msa0467150:three_prime_UTR 50.0%
GTAGGTAGATCGCTTTGACG+GGG + chr3_3:68379506-68379525 None:intergenic 50.0%
GGTAGGTAGATCGCTTTGAC+GGG + chr3_3:68379507-68379526 None:intergenic 50.0%
CATGGCGAAGGTTACGAACA+AGG + chr3_3:68379535-68379554 None:intergenic 50.0%
GTAACCTTCGCCATGACTGT+AGG - chr3_3:68379540-68379559 Msa0467150:three_prime_UTR 50.0%
GATCTTCAGGCCTACAGTCA+TGG + chr3_3:68379553-68379572 None:intergenic 50.0%
! TACAGGCAACCTCGTGGTTT+TGG - chr3_3:68380056-68380075 Msa0467150:intron 50.0%
TCTCTGACCCTCATCTCAGT+TGG + chr3_3:68380197-68380216 None:intergenic 50.0%
AGATGAGGGTCAGAGAGAGA+AGG - chr3_3:68380201-68380220 Msa0467150:CDS 50.0%
AGTCAATCCCATCTCTTCCG+AGG + chr3_3:68380428-68380447 None:intergenic 50.0%
CAACTTTACCAGTTGCGCCA+AGG + chr3_3:68380644-68380663 None:intergenic 50.0%
AGATTCAAACCGAGATCGGC+GGG + chr3_3:68380832-68380851 None:intergenic 50.0%
GGTGAACGAGAATAACGTGG+GGG + chr3_3:68381224-68381243 None:intergenic 50.0%
GGGTGAACGAGAATAACGTG+GGG + chr3_3:68381225-68381244 None:intergenic 50.0%
GGGGTGAACGAGAATAACGT+GGG + chr3_3:68381226-68381245 None:intergenic 50.0%
ATTCCCCTCCTCCTAAACGA+AGG - chr3_3:68381303-68381322 Msa0467150:CDS 50.0%
TTCCCCTCCTCCTAAACGAA+GGG - chr3_3:68381304-68381323 Msa0467150:CDS 50.0%
TTCCCTTCGTTTAGGAGGAG+GGG + chr3_3:68381309-68381328 None:intergenic 50.0%
!! GCTGGACTAGCACATACTGT+TGG - chr3_3:68381426-68381445 Msa0467150:intron 50.0%
! GATAGAAGGCACAGTCGAGA+AGG - chr3_3:68381616-68381635 Msa0467150:intron 50.0%
GATCACAACACAGCAGAGAG+AGG - chr3_3:68381638-68381657 Msa0467150:intron 50.0%
CTTCTCACATCCCCGTTCTT+GGG + chr3_3:68381682-68381701 None:intergenic 50.0%
CGTAGTCAGAGTCCAGTGAA+AGG - chr3_3:68381703-68381722 Msa0467150:intron 50.0%
GAAGAAGTCCAAGTCCTCGC+CGG - chr3_3:68379471-68379490 Msa0467150:three_prime_UTR 55.0%
GCTTTCTACAGGCAACCTCG+TGG - chr3_3:68380050-68380069 Msa0467150:intron 55.0%
AAGGTCACGGGTTCAAGTCC+TGG - chr3_3:68380679-68380698 Msa0467150:intron 55.0%
GTCGGCATTCTGATCGAAGG+AGG - chr3_3:68381195-68381214 Msa0467150:intron 55.0%
CGGGGTGAACGAGAATAACG+TGG + chr3_3:68381227-68381246 None:intergenic 55.0%
ATCTGTGACGAGGTGGAGAC+CGG + chr3_3:68381256-68381275 None:intergenic 55.0%
CGACTGTGCCTTCTATCCTC+AGG + chr3_3:68381613-68381632 None:intergenic 55.0%
TCACGCTCTCCACCCAAGAA+CGG - chr3_3:68381667-68381686 Msa0467150:intron 55.0%
GCTTCTCACATCCCCGTTCT+TGG + chr3_3:68381683-68381702 None:intergenic 55.0%
CTCTCGACTGAGCCTTTCAC+TGG + chr3_3:68381718-68381737 None:intergenic 55.0%
AAGTCCGAGCCGTGATGTCA+GGG - chr3_3:68381747-68381766 Msa0467150:intron 55.0%
GGGATGCTTCCCTGACATCA+CGG + chr3_3:68381759-68381778 None:intergenic 55.0%
ACTGACTGGGAGACCTACTG+CGG + chr3_3:68381780-68381799 None:intergenic 55.0%
GGTCTCCCAGTCAGTGACAA+TGG - chr3_3:68381785-68381804 Msa0467150:CDS 55.0%
!! CTGTAACGCGGTGGTGGAGA+TGG + chr3_3:68379431-68379450 None:intergenic 60.0%
CTTCTGCTGTAACGCGGTGG+TGG + chr3_3:68379437-68379456 None:intergenic 60.0%
CCTCTTCTGCTGTAACGCGG+TGG + chr3_3:68379440-68379459 None:intergenic 60.0%
CCACCGCGTTACAGCAGAAG+AGG - chr3_3:68379437-68379456 Msa0467150:three_prime_UTR 60.0%
CCGCCTCTTCTGCTGTAACG+CGG + chr3_3:68379443-68379462 None:intergenic 60.0%
CCGCGTTACAGCAGAAGAGG+CGG - chr3_3:68379440-68379459 Msa0467150:three_prime_UTR 60.0%
CGTTACAGCAGAAGAGGCGG+CGG - chr3_3:68379443-68379462 Msa0467150:three_prime_UTR 60.0%
! TTTGACGGGGAGCGTAACGC+CGG + chr3_3:68379493-68379512 None:intergenic 60.0%
CAGGCCTACAGTCATGGCGA+AGG + chr3_3:68379547-68379566 None:intergenic 60.0%
TTCGCATCAGCAGCATCCGC+AGG + chr3_3:68380101-68380120 None:intergenic 60.0%
AGGGGTAACCTTGGCGCAAC+TGG - chr3_3:68380633-68380652 Msa0467150:intron 60.0%
! CAAATGGTGGGACCCCTTCC+CGG - chr3_3:68380748-68380767 Msa0467150:intron 60.0%
CTGAAGCTCCCGCATACGCA+GGG + chr3_3:68380775-68380794 None:intergenic 60.0%
ACTGAAGCTCCCGCATACGC+AGG + chr3_3:68380776-68380795 None:intergenic 60.0%
ATGCGGGAGCTTCAGTGCAC+CGG - chr3_3:68380780-68380799 Msa0467150:intron 60.0%
GCGGTCGGCATTCTGATCGA+AGG - chr3_3:68381192-68381211 Msa0467150:intron 60.0%
CTCGTTCACCCCGCTATTCC+CGG - chr3_3:68381234-68381253 Msa0467150:CDS 60.0%
AGGTGGAGACCGGGAATAGC+GGG + chr3_3:68381246-68381265 None:intergenic 60.0%
GAGGTGGAGACCGGGAATAG+CGG + chr3_3:68381247-68381266 None:intergenic 60.0%
TCTGTGACGAGGTGGAGACC+GGG + chr3_3:68381255-68381274 None:intergenic 60.0%
GAGCGAGATCTGTGACGAGG+TGG + chr3_3:68381263-68381282 None:intergenic 60.0%
CGAGAGCGAGATCTGTGACG+AGG + chr3_3:68381266-68381285 None:intergenic 60.0%
CGTCACAGATCTCGCTCTCG+CGG - chr3_3:68381266-68381285 Msa0467150:CDS 60.0%
CACGCTCTCCACCCAAGAAC+GGG - chr3_3:68381668-68381687 Msa0467150:intron 60.0%
ACGCTCTCCACCCAAGAACG+GGG - chr3_3:68381669-68381688 Msa0467150:intron 60.0%
CTCACATCCCCGTTCTTGGG+TGG + chr3_3:68381679-68381698 None:intergenic 60.0%
! CTGACATCACGGCTCGGACT+TGG + chr3_3:68381748-68381767 None:intergenic 60.0%
CAAGTCCGAGCCGTGATGTC+AGG - chr3_3:68381746-68381765 Msa0467150:intron 60.0%
GCTTCCCTGACATCACGGCT+CGG + chr3_3:68381754-68381773 None:intergenic 60.0%
!! TCAGGGAAGCATCCCGCAGT+AGG - chr3_3:68381764-68381783 Msa0467150:intron 60.0%
CTGACTGGGAGACCTACTGC+GGG + chr3_3:68381779-68381798 None:intergenic 60.0%
TACAGCAGAAGAGGCGGCGG+AGG - chr3_3:68379446-68379465 Msa0467150:three_prime_UTR 65.0%
CGGGAAGGGGTCCCACCATT+TGG + chr3_3:68380750-68380769 None:intergenic 65.0%
CATACGCAGGGTCCGGGAAG+GGG + chr3_3:68380763-68380782 None:intergenic 65.0%
GCATACGCAGGGTCCGGGAA+GGG + chr3_3:68380764-68380783 None:intergenic 65.0%
CTTCCCGGACCCTGCGTATG+CGG - chr3_3:68380763-68380782 Msa0467150:intron 65.0%
TTCCCGGACCCTGCGTATGC+GGG - chr3_3:68380764-68380783 Msa0467150:intron 65.0%
TGCGGGAGCTTCAGTGCACC+GGG - chr3_3:68380781-68380800 Msa0467150:intron 65.0%
GGTGGAGACCGGGAATAGCG+GGG + chr3_3:68381245-68381264 None:intergenic 65.0%
AGCAGAAGAGGCGGCGGAGG+AGG - chr3_3:68379449-68379468 Msa0467150:three_prime_UTR 70.0%
GCGTAACGCCGGCGAGGACT+TGG + chr3_3:68379482-68379501 None:intergenic 70.0%
CGCATACGCAGGGTCCGGGA+AGG + chr3_3:68380765-68380784 None:intergenic 70.0%
CTCCCGCATACGCAGGGTCC+GGG + chr3_3:68380769-68380788 None:intergenic 70.0%
GCTCCCGCATACGCAGGGTC+CGG + chr3_3:68380770-68380789 None:intergenic 70.0%
!! CGGGGAGCGTAACGCCGGCG+AGG + chr3_3:68379488-68379507 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 68379339 68382081 68379339 ID=Msa0467150;Name=Msa0467150
chr3_3 mRNA 68379339 68382081 68379339 ID=Msa0467150-mRNA-1;Parent=Msa0467150;Name=Msa0467150-mRNA-1;_AED=0.11;_eAED=0.11;_QI=68|1|1|1|1|1|4|280|247
chr3_3 exon 68379339 68379820 68379339 ID=Msa0467150-mRNA-1:exon:15831;Parent=Msa0467150-mRNA-1
chr3_3 exon 68380084 68380230 68380084 ID=Msa0467150-mRNA-1:exon:15830;Parent=Msa0467150-mRNA-1
chr3_3 exon 68381198 68381359 68381198 ID=Msa0467150-mRNA-1:exon:15829;Parent=Msa0467150-mRNA-1
chr3_3 exon 68381781 68382081 68381781 ID=Msa0467150-mRNA-1:exon:15828;Parent=Msa0467150-mRNA-1
chr3_3 five_prime_UTR 68382014 68382081 68382014 ID=Msa0467150-mRNA-1:five_prime_utr;Parent=Msa0467150-mRNA-1
chr3_3 CDS 68381781 68382013 68381781 ID=Msa0467150-mRNA-1:cds;Parent=Msa0467150-mRNA-1
chr3_3 CDS 68381198 68381359 68381198 ID=Msa0467150-mRNA-1:cds;Parent=Msa0467150-mRNA-1
chr3_3 CDS 68380084 68380230 68380084 ID=Msa0467150-mRNA-1:cds;Parent=Msa0467150-mRNA-1
chr3_3 CDS 68379619 68379820 68379619 ID=Msa0467150-mRNA-1:cds;Parent=Msa0467150-mRNA-1
chr3_3 three_prime_UTR 68379339 68379618 68379339 ID=Msa0467150-mRNA-1:three_prime_utr;Parent=Msa0467150-mRNA-1
Gene Sequence

>Msa0467150

ATGAGAGGAAGAAGCTACAGTCCATCTCCACCACCGCGTTACAGCAGAAGAGGCGGCGGAGGAGGAAGAAGTCCAAGTCCTCGCCGGCGTTACGCTCCCCGTCAAAGCGATCTACCTACCAGTCTCCTTGTTCGTAACCTTCGCCATGACTGTAGGCCTGAAGATCTCCGTAGACCTTTCGGTCATTTTGGTCCTCTTAAGGATATTTATCTCCCCAAAGATTATTACACTGGGCAACCTCGTGGTTTTGGTTTTATTCAATTTGTGGATCCTGCGGATGCTGCTGATGCGAAATATCATATGGATGGTCAAGTTCTTCTTGGTAGGGAGATAACTGTTGTTTTTGCTGAGGAAAATAGAAAGAAGCCAACTGAGATGAGGGTCAGAGAGAGAAGTAGCGGTCGGCATTCTGATCGAAGGAGGTCTCCCCCACGTTATTCTCGTTCACCCCGCTATTCCCGGTCTCCACCTCGTCACAGATCTCGCTCTCGCGGCAGTCGTGACTATTATTCCCCTCCTCCTAAACGAAGGGAATATTCAAGATCTGTATCACCTGAGGATAGAAGGCACAGTCGAGAAGGATCACAACACAGCAGAGAGAGGTCATACTCACGCTCTCCACCCAAGAACGGGGATGTGAGAAGCCGTAGTCAGAGTCCAGTGAAAGGCTCAGTCGAGAGCAGAAGTCCAAGTCCGAGCCGTGATGTCAGGGAAGCATCCCGCAGTAGGTCTCCCAGTCAGTGA
Protein sequence

>Msa0467150

MRGRSYSPSPPPRYSRRGGGGGRSPSPRRRYAPRQSDLPTSLLVRNLRHDCRPEDLRRPFGHFGPLKDIYLPKDYYTGQPRGFGFIQFVDPADAADAKYHMDGQVLLGREITVVFAEENRKKPTEMRVRERSSGRHSDRRRSPPRYSRSPRYSRSPPRHRSRSRGSRDYYSPPPKRREYSRSVSPEDRRHSREGSQHSRERSYSRSPPKNGDVRSRSQSPVKGSVESRSPSPSRDVREASRSRSPSQ*