AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0467730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0467730 MtrunA17_Chr3g0122651 98.765 81 1 0 13 93 1 81 1.84e-54 163
Msa0467730 MtrunA17_Chr8g0377251 79.012 81 17 0 13 93 1 81 3.20e-44 137
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0467730 AT4G40042.1 79.747 79 16 0 1 79 1 79 1.31e-43 137
Msa0467730 AT2G22425.1 71.591 88 25 0 1 88 1 88 1.83e-41 131
Msa0467730 AT2G22425.2 71.591 88 25 0 1 88 1 88 1.83e-41 131

Find 52 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTTACCCTACTTCTTAA+TGG 0.179148 3_3:+69166180 None:intergenic
TTGAAATTTCATGCTTTAAC+AGG 0.261664 3_3:-69166130 Msa0467730:three_prime_UTR
GAGAGGATGGCGGTTGAAAA+AGG 0.272451 3_3:+69166279 None:intergenic
AATCAAACCTAAAACGAAAA+TGG 0.290194 3_3:+69166011 None:intergenic
CATTGGTGACTGTCCCAAAT+TGG 0.297025 3_3:-69166303 Msa0467730:CDS
TCTTTACCCTACTTCTTAAT+GGG 0.300035 3_3:+69166181 None:intergenic
GCATTTGCTGTGGTTGCATT+TGG 0.308000 3_3:-69166394 Msa0467730:CDS
TTTGATCCAAACCATGGTTT+TGG 0.344806 3_3:-69165848 Msa0467730:three_prime_UTR
GAACTTAGCTTCTCACTGTT+TGG 0.352853 3_3:-69165921 Msa0467730:three_prime_UTR
AGTAAAAGCTTGATTCTTGC+AGG 0.355465 3_3:-69165878 Msa0467730:three_prime_UTR
TTAACACCAAAACCATGGTT+TGG 0.411079 3_3:+69165842 None:intergenic
TTCTTCTTTGAATTCACAAT+AGG 0.417935 3_3:+69166205 None:intergenic
AGATATTTACCGTCGTAAAC+AGG 0.428991 3_3:+69165964 None:intergenic
GAAGAAACCCATTAAGAAGT+AGG 0.438658 3_3:-69166188 Msa0467730:exon
TCTGCATTAGCTTCTCTGCT+AGG 0.440951 3_3:+69166428 None:intergenic
GGTAAATATCTTCTAGATGC+TGG 0.449457 3_3:-69165952 Msa0467730:three_prime_UTR
ATAGGAGATAGCTGTGGCTT+CGG 0.450784 3_3:+69166223 None:intergenic
GCTGTGGTTGCATTTGGAAC+GGG 0.459654 3_3:-69166388 Msa0467730:CDS
CAACCACGTCATCCTTCATC+TGG 0.474353 3_3:-69167687 None:intergenic
AAAGCATGAAATTTCAACTC+AGG 0.476370 3_3:+69166136 None:intergenic
TGTTTGGAACGTGCCAATCA+TGG 0.484193 3_3:-69165905 Msa0467730:three_prime_UTR
TATGCTTCCCTACCAGATGA+AGG 0.491681 3_3:+69167675 None:intergenic
TGCTGTGGTTGCATTTGGAA+CGG 0.510809 3_3:-69166389 Msa0467730:CDS
AAGAAACCCATTAAGAAGTA+GGG 0.531311 3_3:-69166187 Msa0467730:exon
ACCGTCGTAAACAGGCATAA+AGG 0.531482 3_3:+69165972 None:intergenic
TTCATCAGATGGATTGGCAA+GGG 0.536217 3_3:-69166456 Msa0467730:intron
CCGCCATCCTCTCAAGTGGT+TGG 0.536748 3_3:-69166269 Msa0467730:CDS
AAGCATGAAATTTCAACTCA+GGG 0.549473 3_3:+69166137 None:intergenic
TCAACCGCCATCCTCTCAAG+TGG 0.553865 3_3:-69166273 Msa0467730:CDS
ACGTCATCCTTCATCTGGTA+GGG 0.560439 3_3:-69167682 Msa0467730:five_prime_UTR
GCCTTTATGCCTGTTTACGA+CGG 0.563083 3_3:-69165973 Msa0467730:three_prime_UTR
GATCCAACCACTTGAGAGGA+TGG 0.566183 3_3:+69166266 None:intergenic
CTTCATCAGATGGATTGGCA+AGG 0.566563 3_3:-69166457 Msa0467730:intron
CTTGGATCCAACCACTTGAG+AGG 0.567895 3_3:+69166262 None:intergenic
TTTGTGATAATATCCACCAA+GGG 0.575904 3_3:+69166075 None:intergenic
CACGTCATCCTTCATCTGGT+AGG 0.580036 3_3:-69167683 Msa0467730:five_prime_UTR
AAACGTGAATTGGGACGAGA+AGG 0.592323 3_3:+69167596 None:intergenic
AATGTTGCTTGCATTTGCTG+TGG 0.601891 3_3:-69166404 Msa0467730:CDS
TGTAGCTTTGATCCAAACCA+TGG 0.603465 3_3:-69165854 Msa0467730:three_prime_UTR
TATACTTAACACCAAAACCA+TGG 0.611271 3_3:+69165837 None:intergenic
ATCTTCTAGATGCTGGAGAG+AGG 0.612488 3_3:-69165945 Msa0467730:three_prime_UTR
ATTTGTGATAATATCCACCA+AGG 0.616830 3_3:+69166074 None:intergenic
ACGATGATGCTCATATATGC+TGG 0.624836 3_3:-69166343 Msa0467730:CDS
TGGTGTTGTTCTCACCACAT+TGG 0.662379 3_3:-69166320 Msa0467730:CDS
CAAGTGGTTGGATCCAAGTG+AGG 0.664517 3_3:-69166257 Msa0467730:CDS
GTGATAATATCCACCAAGGG+GGG 0.670450 3_3:+69166078 None:intergenic
TTGTGATAATATCCACCAAG+GGG 0.686448 3_3:+69166076 None:intergenic
ATGATGCTCATATATGCTGG+TGG 0.699379 3_3:-69166340 Msa0467730:CDS
TGTGATAATATCCACCAAGG+GGG 0.722617 3_3:+69166077 None:intergenic
CCAACCACTTGAGAGGATGG+CGG 0.730520 3_3:+69166269 None:intergenic
ATTTGGGACAGTCACCAATG+TGG 0.730637 3_3:+69166306 None:intergenic
TTCACAATAGGAGATAGCTG+TGG 0.732645 3_3:+69166217 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GGAATATAAATATTAAATTT+TGG - chr3_3:69166787-69166806 Msa0467730:intron 10.0%
!!! GGAATATAAATATTAAATTT+TGG - chr3_3:69166787-69166806 Msa0467730:intron 10.0%
!! AGAAAAGAGTTACAAAAAAA+GGG + chr3_3:69165877-69165896 None:intergenic 20.0%
!! AAGAAAAGAGTTACAAAAAA+AGG + chr3_3:69165878-69165897 None:intergenic 20.0%
!! TTATAGAATGAAAATTGAAG+AGG + chr3_3:69165989-69166008 None:intergenic 20.0%
!! ATTACAAATGTAGTTTGATT+AGG - chr3_3:69166161-69166180 Msa0467730:three_prime_UTR 20.0%
!!! TTTTATCAATTTAGGGTTTA+GGG - chr3_3:69166208-69166227 Msa0467730:CDS 20.0%
!!! TCCTCATTTTAACATATATA+TGG - chr3_3:69166663-69166682 Msa0467730:intron 20.0%
!!! TACAATGTACTTTACTTTTT+TGG - chr3_3:69167472-69167491 Msa0467730:intron 20.0%
!! AGAAAAGAGTTACAAAAAAA+GGG + chr3_3:69165877-69165896 None:intergenic 20.0%
!! AAGAAAAGAGTTACAAAAAA+AGG + chr3_3:69165878-69165897 None:intergenic 20.0%
!! TTATAGAATGAAAATTGAAG+AGG + chr3_3:69165989-69166008 None:intergenic 20.0%
!! ATTACAAATGTAGTTTGATT+AGG - chr3_3:69166161-69166180 Msa0467730:three_prime_UTR 20.0%
!!! TTTTATCAATTTAGGGTTTA+GGG - chr3_3:69166208-69166227 Msa0467730:CDS 20.0%
!!! TCCTCATTTTAACATATATA+TGG - chr3_3:69166663-69166682 Msa0467730:intron 20.0%
!!! TACAATGTACTTTACTTTTT+TGG - chr3_3:69167472-69167491 Msa0467730:intron 20.0%
! GAAAAGAGTTACAAAAAAAG+GGG + chr3_3:69165876-69165895 None:intergenic 25.0%
! GAAGAAAAAAAACGTGAATT+GGG + chr3_3:69165938-69165957 None:intergenic 25.0%
! TATTGTGGATATTGTGAATT+TGG - chr3_3:69166130-69166149 Msa0467730:three_prime_UTR 25.0%
! ATTGTGGATATTGTGAATTT+GGG - chr3_3:69166131-69166150 Msa0467730:three_prime_UTR 25.0%
!! TCGATCCTTTTATCAATTTA+GGG - chr3_3:69166201-69166220 Msa0467730:CDS 25.0%
! CTAAACCCTAAATTGATAAA+AGG + chr3_3:69166209-69166228 None:intergenic 25.0%
!!! CTTTTATCAATTTAGGGTTT+AGG - chr3_3:69166207-69166226 Msa0467730:CDS 25.0%
! AAAATCCATTCATTCCAAAT+CGG - chr3_3:69166526-69166545 Msa0467730:intron 25.0%
! GCCATATATATGTTAAAATG+AGG + chr3_3:69166667-69166686 None:intergenic 25.0%
!!! TAACATATATATGGCTTGTT+CGG - chr3_3:69166672-69166691 Msa0467730:intron 25.0%
! TTTGCTTGGAAATGAATAAA+TGG - chr3_3:69166766-69166785 Msa0467730:intron 25.0%
! GAAAATATTGAATTCATCCA+AGG + chr3_3:69166857-69166876 None:intergenic 25.0%
! TCTGATGAAGAAAAAAGTTA+AGG + chr3_3:69167055-69167074 None:intergenic 25.0%
!!! TAACTTTTTTCTTCATCAGA+TGG - chr3_3:69167055-69167074 Msa0467730:intron 25.0%
! TTCTTCTTTGAATTCACAAT+AGG + chr3_3:69167320-69167339 None:intergenic 25.0%
! TTGAAATTTCATGCTTTAAC+AGG - chr3_3:69167392-69167411 Msa0467730:intron 25.0%
!!! ATATTAGCCATTTTCGTTTT+AGG - chr3_3:69167504-69167523 Msa0467730:intron 25.0%
! AATCAAACCTAAAACGAAAA+TGG + chr3_3:69167514-69167533 None:intergenic 25.0%
! GAAAAGAGTTACAAAAAAAG+GGG + chr3_3:69165876-69165895 None:intergenic 25.0%
! GAAGAAAAAAAACGTGAATT+GGG + chr3_3:69165938-69165957 None:intergenic 25.0%
! TATTGTGGATATTGTGAATT+TGG - chr3_3:69166130-69166149 Msa0467730:three_prime_UTR 25.0%
! ATTGTGGATATTGTGAATTT+GGG - chr3_3:69166131-69166150 Msa0467730:three_prime_UTR 25.0%
!! TCGATCCTTTTATCAATTTA+GGG - chr3_3:69166201-69166220 Msa0467730:CDS 25.0%
! CTAAACCCTAAATTGATAAA+AGG + chr3_3:69166209-69166228 None:intergenic 25.0%
!!! CTTTTATCAATTTAGGGTTT+AGG - chr3_3:69166207-69166226 Msa0467730:CDS 25.0%
! AAAATCCATTCATTCCAAAT+CGG - chr3_3:69166526-69166545 Msa0467730:intron 25.0%
! GCCATATATATGTTAAAATG+AGG + chr3_3:69166667-69166686 None:intergenic 25.0%
!!! TAACATATATATGGCTTGTT+CGG - chr3_3:69166672-69166691 Msa0467730:intron 25.0%
! TTTGCTTGGAAATGAATAAA+TGG - chr3_3:69166766-69166785 Msa0467730:intron 25.0%
! GAAAATATTGAATTCATCCA+AGG + chr3_3:69166857-69166876 None:intergenic 25.0%
! TCTGATGAAGAAAAAAGTTA+AGG + chr3_3:69167055-69167074 None:intergenic 25.0%
!!! TAACTTTTTTCTTCATCAGA+TGG - chr3_3:69167055-69167074 Msa0467730:intron 25.0%
! TTCTTCTTTGAATTCACAAT+AGG + chr3_3:69167320-69167339 None:intergenic 25.0%
! TTGAAATTTCATGCTTTAAC+AGG - chr3_3:69167392-69167411 Msa0467730:intron 25.0%
!!! ATATTAGCCATTTTCGTTTT+AGG - chr3_3:69167504-69167523 Msa0467730:intron 25.0%
! AATCAAACCTAAAACGAAAA+TGG + chr3_3:69167514-69167533 None:intergenic 25.0%
GGAAGAAAAAAAACGTGAAT+TGG + chr3_3:69165939-69165958 None:intergenic 30.0%
!!! TCACGTTTTTTTTCTTCCAA+AGG - chr3_3:69165941-69165960 Msa0467730:three_prime_UTR 30.0%
AGAAAGAAAGATGTTACCTT+TGG + chr3_3:69165960-69165979 None:intergenic 30.0%
CACGCAACAAAAAATTTGAA+TGG + chr3_3:69166088-69166107 None:intergenic 30.0%
TGATTAGGAACTGATGAATT+TGG - chr3_3:69166176-69166195 Msa0467730:exon 30.0%
! CTCGATCCTTTTATCAATTT+AGG - chr3_3:69166200-69166219 Msa0467730:CDS 30.0%
AATATACTCGTGTAAAGATG+AGG + chr3_3:69166454-69166473 None:intergenic 30.0%
!! AAAAACCGATTTGGAATGAA+TGG + chr3_3:69166534-69166553 None:intergenic 30.0%
!!! TGCCATTTTGTTGTATGTTT+TGG - chr3_3:69166598-69166617 Msa0467730:intron 30.0%
!! GCCATTTTGTTGTATGTTTT+GGG - chr3_3:69166599-69166618 Msa0467730:intron 30.0%
ACCCAAAACATACAACAAAA+TGG + chr3_3:69166603-69166622 None:intergenic 30.0%
AAATGAGGATGATTTCTGAA+TGG + chr3_3:69166652-69166671 None:intergenic 30.0%
!! AACATATATATGGCTTGTTC+GGG - chr3_3:69166673-69166692 Msa0467730:intron 30.0%
CTGTAGTACTTGCAATTATT+CGG - chr3_3:69166711-69166730 Msa0467730:intron 30.0%
! TAGTATCTTCATGTTTTGCT+TGG - chr3_3:69166752-69166771 Msa0467730:intron 30.0%
AAGAATAGTATATAGCTCCT+TGG - chr3_3:69166837-69166856 Msa0467730:intron 30.0%
TTAGAGTTAGAAGTAGTAGT+AGG - chr3_3:69166967-69166986 Msa0467730:intron 30.0%
! TTTTTCTTCATCAGATGGAT+TGG - chr3_3:69167060-69167079 Msa0467730:intron 30.0%
AAGAAACCCATTAAGAAGTA+GGG - chr3_3:69167335-69167354 Msa0467730:intron 30.0%
TCTTTACCCTACTTCTTAAT+GGG + chr3_3:69167344-69167363 None:intergenic 30.0%
TTCTTTACCCTACTTCTTAA+TGG + chr3_3:69167345-69167364 None:intergenic 30.0%
AAGCATGAAATTTCAACTCA+GGG + chr3_3:69167388-69167407 None:intergenic 30.0%
AAAGCATGAAATTTCAACTC+AGG + chr3_3:69167389-69167408 None:intergenic 30.0%
TTTGTGATAATATCCACCAA+GGG + chr3_3:69167450-69167469 None:intergenic 30.0%
ATTTGTGATAATATCCACCA+AGG + chr3_3:69167451-69167470 None:intergenic 30.0%
!!! GTTTTAGGTTTGATTTGTGT+TGG - chr3_3:69167519-69167538 Msa0467730:intron 30.0%
!!! TTTGATTTGTGTTGGTTTTC+TGG - chr3_3:69167527-69167546 Msa0467730:intron 30.0%
GGAAGAAAAAAAACGTGAAT+TGG + chr3_3:69165939-69165958 None:intergenic 30.0%
!!! TCACGTTTTTTTTCTTCCAA+AGG - chr3_3:69165941-69165960 Msa0467730:three_prime_UTR 30.0%
AGAAAGAAAGATGTTACCTT+TGG + chr3_3:69165960-69165979 None:intergenic 30.0%
CACGCAACAAAAAATTTGAA+TGG + chr3_3:69166088-69166107 None:intergenic 30.0%
TGATTAGGAACTGATGAATT+TGG - chr3_3:69166176-69166195 Msa0467730:exon 30.0%
! CTCGATCCTTTTATCAATTT+AGG - chr3_3:69166200-69166219 Msa0467730:CDS 30.0%
AATATACTCGTGTAAAGATG+AGG + chr3_3:69166454-69166473 None:intergenic 30.0%
!! AAAAACCGATTTGGAATGAA+TGG + chr3_3:69166534-69166553 None:intergenic 30.0%
!!! TGCCATTTTGTTGTATGTTT+TGG - chr3_3:69166598-69166617 Msa0467730:intron 30.0%
!! GCCATTTTGTTGTATGTTTT+GGG - chr3_3:69166599-69166618 Msa0467730:intron 30.0%
ACCCAAAACATACAACAAAA+TGG + chr3_3:69166603-69166622 None:intergenic 30.0%
AAATGAGGATGATTTCTGAA+TGG + chr3_3:69166652-69166671 None:intergenic 30.0%
!! AACATATATATGGCTTGTTC+GGG - chr3_3:69166673-69166692 Msa0467730:intron 30.0%
CTGTAGTACTTGCAATTATT+CGG - chr3_3:69166711-69166730 Msa0467730:intron 30.0%
! TAGTATCTTCATGTTTTGCT+TGG - chr3_3:69166752-69166771 Msa0467730:intron 30.0%
AAGAATAGTATATAGCTCCT+TGG - chr3_3:69166837-69166856 Msa0467730:intron 30.0%
TTAGAGTTAGAAGTAGTAGT+AGG - chr3_3:69166967-69166986 Msa0467730:intron 30.0%
! TTTTTCTTCATCAGATGGAT+TGG - chr3_3:69167060-69167079 Msa0467730:intron 30.0%
AAGAAACCCATTAAGAAGTA+GGG - chr3_3:69167335-69167354 Msa0467730:intron 30.0%
TCTTTACCCTACTTCTTAAT+GGG + chr3_3:69167344-69167363 None:intergenic 30.0%
TTCTTTACCCTACTTCTTAA+TGG + chr3_3:69167345-69167364 None:intergenic 30.0%
AAGCATGAAATTTCAACTCA+GGG + chr3_3:69167388-69167407 None:intergenic 30.0%
AAAGCATGAAATTTCAACTC+AGG + chr3_3:69167389-69167408 None:intergenic 30.0%
TTTGTGATAATATCCACCAA+GGG + chr3_3:69167450-69167469 None:intergenic 30.0%
ATTTGTGATAATATCCACCA+AGG + chr3_3:69167451-69167470 None:intergenic 30.0%
!!! GTTTTAGGTTTGATTTGTGT+TGG - chr3_3:69167519-69167538 Msa0467730:intron 30.0%
!!! TTTGATTTGTGTTGGTTTTC+TGG - chr3_3:69167527-69167546 Msa0467730:intron 30.0%
! GAACGAAAGAAAACCGTTTT+TGG + chr3_3:69166028-69166047 None:intergenic 35.0%
! TATAACGAGTGTCACTATTG+TGG - chr3_3:69166115-69166134 Msa0467730:three_prime_UTR 35.0%
CGCAAATTTGAACTAGATCT+CGG + chr3_3:69166303-69166322 None:intergenic 35.0%
TGCGATATCAAAAACCGATT+TGG + chr3_3:69166543-69166562 None:intergenic 35.0%
! CGAGACAAATCTTTTGTGTA+TGG + chr3_3:69166569-69166588 None:intergenic 35.0%
!! ACATATATATGGCTTGTTCG+GGG - chr3_3:69166674-69166693 Msa0467730:intron 35.0%
TGGAACATGAAAGATTCTTG+TGG - chr3_3:69166807-69166826 Msa0467730:intron 35.0%
GAAGAAACCCATTAAGAAGT+AGG - chr3_3:69167334-69167353 Msa0467730:intron 35.0%
TTGTGATAATATCCACCAAG+GGG + chr3_3:69167449-69167468 None:intergenic 35.0%
AGATATTTACCGTCGTAAAC+AGG + chr3_3:69167561-69167580 None:intergenic 35.0%
GGTAAATATCTTCTAGATGC+TGG - chr3_3:69167570-69167589 Msa0467730:intron 35.0%
! GCTTTTACTCAAACCATGAT+TGG + chr3_3:69167633-69167652 None:intergenic 35.0%
AGTAAAAGCTTGATTCTTGC+AGG - chr3_3:69167644-69167663 Msa0467730:five_prime_UTR 35.0%
! TTTGATCCAAACCATGGTTT+TGG - chr3_3:69167674-69167693 Msa0467730:five_prime_UTR 35.0%
TTAACACCAAAACCATGGTT+TGG + chr3_3:69167683-69167702 None:intergenic 35.0%
! GAACGAAAGAAAACCGTTTT+TGG + chr3_3:69166028-69166047 None:intergenic 35.0%
! TATAACGAGTGTCACTATTG+TGG - chr3_3:69166115-69166134 Msa0467730:three_prime_UTR 35.0%
CGCAAATTTGAACTAGATCT+CGG + chr3_3:69166303-69166322 None:intergenic 35.0%
TGCGATATCAAAAACCGATT+TGG + chr3_3:69166543-69166562 None:intergenic 35.0%
! CGAGACAAATCTTTTGTGTA+TGG + chr3_3:69166569-69166588 None:intergenic 35.0%
!! ACATATATATGGCTTGTTCG+GGG - chr3_3:69166674-69166693 Msa0467730:intron 35.0%
TGGAACATGAAAGATTCTTG+TGG - chr3_3:69166807-69166826 Msa0467730:intron 35.0%
GAAGAAACCCATTAAGAAGT+AGG - chr3_3:69167334-69167353 Msa0467730:intron 35.0%
TTGTGATAATATCCACCAAG+GGG + chr3_3:69167449-69167468 None:intergenic 35.0%
AGATATTTACCGTCGTAAAC+AGG + chr3_3:69167561-69167580 None:intergenic 35.0%
GGTAAATATCTTCTAGATGC+TGG - chr3_3:69167570-69167589 Msa0467730:intron 35.0%
! GCTTTTACTCAAACCATGAT+TGG + chr3_3:69167633-69167652 None:intergenic 35.0%
AGTAAAAGCTTGATTCTTGC+AGG - chr3_3:69167644-69167663 Msa0467730:five_prime_UTR 35.0%
! TTTGATCCAAACCATGGTTT+TGG - chr3_3:69167674-69167693 Msa0467730:five_prime_UTR 35.0%
TTAACACCAAAACCATGGTT+TGG + chr3_3:69167683-69167702 None:intergenic 35.0%
GCGAATTCTTCTGCCAAAAA+CGG - chr3_3:69166012-69166031 Msa0467730:three_prime_UTR 40.0%
TGAGGCGAACAAGAAATTCA+AGG + chr3_3:69166436-69166455 None:intergenic 40.0%
!! TTCATCAGATGGATTGGCAA+GGG - chr3_3:69167066-69167085 Msa0467730:intron 40.0%
! AATGTTGCTTGCATTTGCTG+TGG - chr3_3:69167118-69167137 Msa0467730:intron 40.0%
ACGATGATGCTCATATATGC+TGG - chr3_3:69167179-69167198 Msa0467730:intron 40.0%
ATGATGCTCATATATGCTGG+TGG - chr3_3:69167182-69167201 Msa0467730:intron 40.0%
GGTTGAAAAAGGGCCAATTT+GGG + chr3_3:69167235-69167254 None:intergenic 40.0%
TTCACAATAGGAGATAGCTG+TGG + chr3_3:69167308-69167327 None:intergenic 40.0%
TGTGATAATATCCACCAAGG+GGG + chr3_3:69167448-69167467 None:intergenic 40.0%
GAACTTAGCTTCTCACTGTT+TGG - chr3_3:69167601-69167620 Msa0467730:five_prime_UTR 40.0%
!! TGTAGCTTTGATCCAAACCA+TGG - chr3_3:69167668-69167687 Msa0467730:five_prime_UTR 40.0%
GCGAATTCTTCTGCCAAAAA+CGG - chr3_3:69166012-69166031 Msa0467730:three_prime_UTR 40.0%
TGAGGCGAACAAGAAATTCA+AGG + chr3_3:69166436-69166455 None:intergenic 40.0%
!! TTCATCAGATGGATTGGCAA+GGG - chr3_3:69167066-69167085 Msa0467730:intron 40.0%
! AATGTTGCTTGCATTTGCTG+TGG - chr3_3:69167118-69167137 Msa0467730:intron 40.0%
ACGATGATGCTCATATATGC+TGG - chr3_3:69167179-69167198 Msa0467730:intron 40.0%
ATGATGCTCATATATGCTGG+TGG - chr3_3:69167182-69167201 Msa0467730:intron 40.0%
GGTTGAAAAAGGGCCAATTT+GGG + chr3_3:69167235-69167254 None:intergenic 40.0%
TTCACAATAGGAGATAGCTG+TGG + chr3_3:69167308-69167327 None:intergenic 40.0%
TGTGATAATATCCACCAAGG+GGG + chr3_3:69167448-69167467 None:intergenic 40.0%
GAACTTAGCTTCTCACTGTT+TGG - chr3_3:69167601-69167620 Msa0467730:five_prime_UTR 40.0%
!! TGTAGCTTTGATCCAAACCA+TGG - chr3_3:69167668-69167687 Msa0467730:five_prime_UTR 40.0%
TATGCTTCCCTACCAGATGA+AGG + chr3_3:69165850-69165869 None:intergenic 45.0%
AAACGTGAATTGGGACGAGA+AGG + chr3_3:69165929-69165948 None:intergenic 45.0%
AACTAGATCTCGGCTTAGCA+GGG + chr3_3:69166293-69166312 None:intergenic 45.0%
GCGTGTGTGTTTGATTCTAG+AGG - chr3_3:69166320-69166339 Msa0467730:CDS 45.0%
!! CTTCATCAGATGGATTGGCA+AGG - chr3_3:69167065-69167084 Msa0467730:intron 45.0%
! TCTGCATTAGCTTCTCTGCT+AGG + chr3_3:69167097-69167116 None:intergenic 45.0%
!! GCATTTGCTGTGGTTGCATT+TGG - chr3_3:69167128-69167147 Msa0467730:intron 45.0%
TGCTGTGGTTGCATTTGGAA+CGG - chr3_3:69167133-69167152 Msa0467730:intron 45.0%
!! TGGTGTTGTTCTCACCACAT+TGG - chr3_3:69167202-69167221 Msa0467730:intron 45.0%
! ATTTGGGACAGTCACCAATG+TGG + chr3_3:69167219-69167238 None:intergenic 45.0%
! CATTGGTGACTGTCCCAAAT+TGG - chr3_3:69167219-69167238 Msa0467730:intron 45.0%
CGGTTGAAAAAGGGCCAATT+TGG + chr3_3:69167236-69167255 None:intergenic 45.0%
! AGAGGATGGCGGTTGAAAAA+GGG + chr3_3:69167245-69167264 None:intergenic 45.0%
! GGATGCTTTTCTACCTCACT+TGG + chr3_3:69167281-69167300 None:intergenic 45.0%
ATAGGAGATAGCTGTGGCTT+CGG + chr3_3:69167302-69167321 None:intergenic 45.0%
GTGATAATATCCACCAAGGG+GGG + chr3_3:69167447-69167466 None:intergenic 45.0%
GCCTTTATGCCTGTTTACGA+CGG - chr3_3:69167549-69167568 Msa0467730:intron 45.0%
ACCGTCGTAAACAGGCATAA+AGG + chr3_3:69167553-69167572 None:intergenic 45.0%
ATCTTCTAGATGCTGGAGAG+AGG - chr3_3:69167577-69167596 Msa0467730:intron 45.0%
! TGTTTGGAACGTGCCAATCA+TGG - chr3_3:69167617-69167636 Msa0467730:five_prime_UTR 45.0%
TATGCTTCCCTACCAGATGA+AGG + chr3_3:69165850-69165869 None:intergenic 45.0%
AAACGTGAATTGGGACGAGA+AGG + chr3_3:69165929-69165948 None:intergenic 45.0%
AACTAGATCTCGGCTTAGCA+GGG + chr3_3:69166293-69166312 None:intergenic 45.0%
GCGTGTGTGTTTGATTCTAG+AGG - chr3_3:69166320-69166339 Msa0467730:CDS 45.0%
!! CTTCATCAGATGGATTGGCA+AGG - chr3_3:69167065-69167084 Msa0467730:intron 45.0%
! TCTGCATTAGCTTCTCTGCT+AGG + chr3_3:69167097-69167116 None:intergenic 45.0%
!! GCATTTGCTGTGGTTGCATT+TGG - chr3_3:69167128-69167147 Msa0467730:intron 45.0%
TGCTGTGGTTGCATTTGGAA+CGG - chr3_3:69167133-69167152 Msa0467730:intron 45.0%
!! TGGTGTTGTTCTCACCACAT+TGG - chr3_3:69167202-69167221 Msa0467730:intron 45.0%
! ATTTGGGACAGTCACCAATG+TGG + chr3_3:69167219-69167238 None:intergenic 45.0%
! CATTGGTGACTGTCCCAAAT+TGG - chr3_3:69167219-69167238 Msa0467730:intron 45.0%
CGGTTGAAAAAGGGCCAATT+TGG + chr3_3:69167236-69167255 None:intergenic 45.0%
! AGAGGATGGCGGTTGAAAAA+GGG + chr3_3:69167245-69167264 None:intergenic 45.0%
! GGATGCTTTTCTACCTCACT+TGG + chr3_3:69167281-69167300 None:intergenic 45.0%
ATAGGAGATAGCTGTGGCTT+CGG + chr3_3:69167302-69167321 None:intergenic 45.0%
GTGATAATATCCACCAAGGG+GGG + chr3_3:69167447-69167466 None:intergenic 45.0%
GCCTTTATGCCTGTTTACGA+CGG - chr3_3:69167549-69167568 Msa0467730:intron 45.0%
ACCGTCGTAAACAGGCATAA+AGG + chr3_3:69167553-69167572 None:intergenic 45.0%
ATCTTCTAGATGCTGGAGAG+AGG - chr3_3:69167577-69167596 Msa0467730:intron 45.0%
! TGTTTGGAACGTGCCAATCA+TGG - chr3_3:69167617-69167636 Msa0467730:five_prime_UTR 45.0%
GAACTAGATCTCGGCTTAGC+AGG + chr3_3:69166294-69166313 None:intergenic 50.0%
! GCTGTGGTTGCATTTGGAAC+GGG - chr3_3:69167134-69167153 Msa0467730:intron 50.0%
! GAGAGGATGGCGGTTGAAAA+AGG + chr3_3:69167246-69167265 None:intergenic 50.0%
GATCCAACCACTTGAGAGGA+TGG + chr3_3:69167259-69167278 None:intergenic 50.0%
CTTGGATCCAACCACTTGAG+AGG + chr3_3:69167263-69167282 None:intergenic 50.0%
CAAGTGGTTGGATCCAAGTG+AGG - chr3_3:69167265-69167284 Msa0467730:intron 50.0%
GAACTAGATCTCGGCTTAGC+AGG + chr3_3:69166294-69166313 None:intergenic 50.0%
! GCTGTGGTTGCATTTGGAAC+GGG - chr3_3:69167134-69167153 Msa0467730:intron 50.0%
! GAGAGGATGGCGGTTGAAAA+AGG + chr3_3:69167246-69167265 None:intergenic 50.0%
GATCCAACCACTTGAGAGGA+TGG + chr3_3:69167259-69167278 None:intergenic 50.0%
CTTGGATCCAACCACTTGAG+AGG + chr3_3:69167263-69167282 None:intergenic 50.0%
CAAGTGGTTGGATCCAAGTG+AGG - chr3_3:69167265-69167284 Msa0467730:intron 50.0%
!! ATATGGCTTGTTCGGGGCTC+TGG - chr3_3:69166680-69166699 Msa0467730:intron 55.0%
TCAACCGCCATCCTCTCAAG+TGG - chr3_3:69167249-69167268 Msa0467730:intron 55.0%
CCAACCACTTGAGAGGATGG+CGG + chr3_3:69167256-69167275 None:intergenic 55.0%
!! ATATGGCTTGTTCGGGGCTC+TGG - chr3_3:69166680-69166699 Msa0467730:intron 55.0%
TCAACCGCCATCCTCTCAAG+TGG - chr3_3:69167249-69167268 Msa0467730:intron 55.0%
CCAACCACTTGAGAGGATGG+CGG + chr3_3:69167256-69167275 None:intergenic 55.0%
CCGCCATCCTCTCAAGTGGT+TGG - chr3_3:69167253-69167272 Msa0467730:intron 60.0%
CTTACCCTCTTGTCCCCCCT+TGG - chr3_3:69167431-69167450 Msa0467730:intron 60.0%
TCCACCAAGGGGGGACAAGA+GGG + chr3_3:69167438-69167457 None:intergenic 60.0%
ATCCACCAAGGGGGGACAAG+AGG + chr3_3:69167439-69167458 None:intergenic 60.0%
CCGCCATCCTCTCAAGTGGT+TGG - chr3_3:69167253-69167272 Msa0467730:intron 60.0%
CTTACCCTCTTGTCCCCCCT+TGG - chr3_3:69167431-69167450 Msa0467730:intron 60.0%
TCCACCAAGGGGGGACAAGA+GGG + chr3_3:69167438-69167457 None:intergenic 60.0%
ATCCACCAAGGGGGGACAAG+AGG + chr3_3:69167439-69167458 None:intergenic 60.0%
ACCCTCTTGTCCCCCCTTGG+TGG - chr3_3:69167434-69167453 Msa0467730:intron 65.0%
ACCCTCTTGTCCCCCCTTGG+TGG - chr3_3:69167434-69167453 Msa0467730:intron 65.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 69165842 69167702 69165842 ID=Msa0467730;Name=Msa0467730
chr3_3 mRNA 69165842 69167702 69165842 ID=Msa0467730-mRNA-1;Parent=Msa0467730;Name=Msa0467730-mRNA-1;_AED=0.01;_eAED=0.01;_QI=121|1|1|1|0|0.5|2|347|93
chr3_3 exon 69165842 69166470 69165842 ID=Msa0467730-mRNA-1:exon:16259;Parent=Msa0467730-mRNA-1
chr3_3 exon 69167582 69167702 69167582 ID=Msa0467730-mRNA-1:exon:16258;Parent=Msa0467730-mRNA-1
chr3_3 five_prime_UTR 69167582 69167702 69167582 ID=Msa0467730-mRNA-1:five_prime_utr;Parent=Msa0467730-mRNA-1
chr3_3 CDS 69166189 69166470 69166189 ID=Msa0467730-mRNA-1:cds;Parent=Msa0467730-mRNA-1
chr3_3 three_prime_UTR 69165842 69166188 69165842 ID=Msa0467730-mRNA-1:three_prime_utr;Parent=Msa0467730-mRNA-1
Gene Sequence

>Msa0467730

ATGGATTGGCAAGGGCAAAACCTAGCAGAGAAGCTAATGCAGATAATGTTGCTTGCATTTGCTGTGGTTGCATTTGGAACGGGATATCTTACAGCTTCTTTCAAGACGATGATGCTCATATATGCTGGTGGTGTTGTTCTCACCACATTGGTGACTGTCCCAAATTGGCCCTTTTTCAACCGCCATCCTCTCAAGTGGTTGGATCCAAGTGAGGTAGAAAAGCATCCGAAGCCACAGCTATCTCCTATTGTGAATTCAAAGAAGAAACCCATTAAGAAGTAG
Protein sequence

>Msa0467730

MDWQGQNLAEKLMQIMLLAFAVVAFGTGYLTASFKTMMLIYAGGVVLTTLVTVPNWPFFNRHPLKWLDPSEVEKHPKPQLSPIVNSKKKPIKK*