AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0468260

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0468260 MtrunA17_Chr3g0116431 91.813 513 30 5 1 503 1 511 0.0 952
Msa0468260 MtrunA17_Chr4g0058011 52.813 551 190 13 17 503 25 569 0.0 533
Msa0468260 MtrunA17_Chr7g0249221 36.119 371 204 7 163 503 27 394 2.47e-73 238
Msa0468260 MtrunA17_Chr5g0438891 38.209 335 165 12 163 475 36 350 5.78e-65 216
Msa0468260 MtrunA17_Chr4g0066941 34.021 388 156 9 161 469 41 407 9.90e-60 205
Msa0468260 MtrunA17_Chr2g0286151 34.593 344 183 9 161 468 42 379 2.22e-52 184
Msa0468260 MtrunA17_Chr1g0195891 33.542 319 166 9 156 442 39 343 3.23e-49 172
Msa0468260 MtrunA17_Chr1g0195891 41.880 117 63 2 391 503 90 205 2.66e-20 92.4
Msa0468260 MtrunA17_Chr8g0356241 31.818 374 150 12 160 494 187 494 4.68e-45 165
Msa0468260 MtrunA17_Chr8g0356241 48.810 84 38 1 387 470 185 263 4.56e-17 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0468260 AT3G48440.1 55.152 330 140 6 150 471 93 422 1.55e-125 374
Msa0468260 AT5G63260.1 55.556 342 140 5 163 500 98 431 5.52e-123 367
Msa0468260 AT5G63260.1 42.683 82 42 2 160 239 328 406 7.69e-14 73.9
Msa0468260 AT5G63260.2 53.073 358 140 6 163 500 98 447 6.68e-119 357
Msa0468260 AT5G63260.2 42.683 82 42 2 160 239 344 422 8.94e-14 73.9
Msa0468260 AT3G02830.2 40.187 321 171 6 163 470 17 329 1.04e-72 235
Msa0468260 AT3G02830.2 45.545 101 54 1 391 490 61 161 2.63e-18 87.0
Msa0468260 AT3G02830.2 42.529 87 43 2 163 246 251 333 5.91e-12 67.8
Msa0468260 AT3G02830.1 40.187 321 171 6 163 470 37 349 1.07e-72 236
Msa0468260 AT3G02830.1 45.545 101 54 1 391 490 81 181 2.65e-18 87.4
Msa0468260 AT3G02830.1 42.529 87 43 2 163 246 271 353 5.02e-12 68.2
Msa0468260 AT5G16540.1 40.260 308 156 6 163 470 39 318 4.92e-70 228
Msa0468260 AT5G16540.1 33.158 190 58 6 162 285 83 269 1.00e-17 85.5
Msa0468260 AT5G16540.1 43.529 85 41 2 163 244 240 320 5.44e-12 67.8
Msa0468260 AT5G16540.3 40.777 309 153 7 163 470 18 297 5.57e-70 228
Msa0468260 AT5G16540.3 33.158 190 58 6 162 285 62 248 1.15e-17 85.1
Msa0468260 AT5G16540.3 43.529 85 41 2 163 244 219 299 5.88e-12 67.8
Msa0468260 AT3G06410.1 39.763 337 165 9 161 466 49 378 5.17e-68 226
Msa0468260 AT3G06410.1 46.914 81 41 1 392 470 97 177 1.24e-17 85.9
Msa0468260 AT3G06410.1 46.154 78 42 0 390 467 49 126 8.47e-17 83.2
Msa0468260 AT3G06410.1 34.884 86 49 2 156 238 297 378 1.99e-11 66.6
Msa0468260 AT2G47850.1 37.079 356 187 10 141 469 18 363 1.01e-67 225
Msa0468260 AT2G47850.1 40.000 90 39 2 161 243 284 365 1.81e-12 69.7
Msa0468260 AT2G47850.3 37.079 356 187 10 141 469 18 363 1.01e-67 225
Msa0468260 AT2G47850.3 40.000 90 39 2 161 243 284 365 1.81e-12 69.7
Msa0468260 AT5G18550.3 38.788 330 173 8 161 466 14 338 8.14e-67 222
Msa0468260 AT5G18550.3 50.000 78 39 0 390 467 14 91 2.13e-18 87.8
Msa0468260 AT5G18550.1 38.788 330 173 8 161 466 46 370 1.36e-66 222
Msa0468260 AT5G18550.1 50.000 78 39 0 390 467 46 123 1.65e-18 88.6
Msa0468260 AT5G18550.4 38.788 330 173 8 161 466 46 370 1.38e-66 222
Msa0468260 AT5G18550.4 50.000 78 39 0 390 467 46 123 1.91e-18 88.2
Msa0468260 AT1G04990.1 37.537 341 187 10 140 475 21 340 6.48e-65 216
Msa0468260 AT1G04990.6 37.537 341 187 10 140 475 21 340 6.48e-65 216
Msa0468260 AT1G04990.2 37.537 341 187 10 140 475 21 340 6.48e-65 216
Msa0468260 AT5G16540.2 38.835 309 152 8 163 470 39 311 1.21e-62 209
Msa0468260 AT5G16540.2 33.158 190 58 6 162 285 83 269 1.09e-17 85.1
Msa0468260 AT5G16540.2 43.902 82 38 1 163 244 240 313 1.32e-12 69.7
Msa0468260 AT5G18550.2 36.364 330 168 8 161 466 46 357 1.37e-59 203
Msa0468260 AT5G18550.2 50.000 78 39 0 390 467 46 123 1.37e-18 88.6
Msa0468260 AT2G32930.2 34.857 350 198 10 134 472 12 342 8.19e-59 201
Msa0468260 AT2G32930.3 35.542 332 184 10 152 472 3 315 1.65e-57 197
Msa0468260 AT1G04990.5 35.191 341 175 11 140 475 21 320 1.03e-55 191
Msa0468260 AT2G32930.1 34.571 350 195 11 134 472 12 338 2.57e-55 192
Msa0468260 AT5G16540.4 38.790 281 142 7 163 442 18 269 9.10e-54 182
Msa0468260 AT5G16540.4 49.351 77 39 0 391 467 62 138 7.73e-19 87.0
Msa0468260 AT5G16540.4 33.158 190 58 6 162 285 62 248 8.18e-18 84.0
Msa0468260 AT5G16540.4 46.835 79 39 2 392 469 18 94 4.95e-14 72.8
Msa0468260 AT2G47850.2 32.931 331 209 5 141 469 18 337 1.36e-49 176
Msa0468260 AT2G47850.2 40.000 90 39 2 161 243 258 339 1.10e-12 70.5
Msa0468260 AT1G04990.3 37.313 268 140 10 216 475 12 259 1.17e-46 165
Msa0468260 AT1G04990.3 42.105 76 44 0 210 285 177 252 9.05e-13 69.7
Msa0468260 AT3G12680.1 30.081 369 196 9 142 467 150 499 1.01e-45 167
Msa0468260 AT3G12680.1 31.118 331 161 10 129 421 198 499 3.18e-36 141
Msa0468260 AT3G12680.1 36.095 169 64 3 164 290 338 504 1.82e-25 110
Msa0468260 AT3G12680.1 45.977 87 42 2 387 471 217 300 1.26e-16 83.2
Msa0468260 AT2G47850.4 38.618 246 118 8 249 469 4 241 4.32e-43 156
Msa0468260 AT2G47850.4 41.935 93 44 1 193 285 157 239 2.37e-17 84.0
Msa0468260 AT2G47850.4 40.000 90 39 2 161 243 162 243 3.88e-13 71.2
Msa0468260 AT1G04990.4 34.328 268 128 11 216 475 12 239 1.15e-37 140
Msa0468260 AT1G48195.1 64.935 77 27 0 390 466 4 80 4.53e-32 118
Msa0468260 AT1G48195.1 38.961 77 47 0 208 284 4 80 9.00e-14 67.0
Msa0468260 AT1G48195.1 41.250 80 42 2 161 238 4 80 9.64e-11 58.5

Find 160 sgRNAs with CRISPR-Local

Find 291 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGATATGGAATTTGCAAATT+TGG 0.116818 3_3:-69870062 Msa0468260:CDS
TCAGGTTCACCAGGTCTTTC+TGG 0.127881 3_3:+69870327 None:intergenic
TCAATCAAATTTCAACTATT+AGG 0.136320 3_3:-69873325 Msa0468260:five_prime_UTR
AAGACTGGAACTTGCAAATT+TGG 0.144169 3_3:-69872739 Msa0468260:CDS
CAATCAAATTTCAACTATTA+GGG 0.227874 3_3:-69873324 Msa0468260:five_prime_UTR
GAACCGGTGCGCATGTAATA+AGG 0.235921 3_3:+69871436 None:intergenic
TTGAAAGAGTGAGAGAAATA+AGG 0.254892 3_3:+69873351 None:intergenic
CAAAAGAACTGCTCAATTAA+TGG 0.266115 3_3:-69869575 Msa0468260:three_prime_UTR
TTCCAGTTTGTTATCTCTCT+TGG 0.278370 3_3:-69869702 Msa0468260:three_prime_UTR
AAGATGAAAATCACAAAATA+TGG 0.299144 3_3:+69869842 None:intergenic
AAAATACCTTGCATTCTATC+TGG 0.302106 3_3:+69872403 None:intergenic
CGCACCGGTTCTTGTAAGTT+TGG 0.307678 3_3:-69871424 Msa0468260:CDS
GGCAGGAGAAAGAGATGAAT+TGG 0.309939 3_3:-69872439 Msa0468260:CDS
AGCAGCTGTAGATTATCTTT+TGG 0.310382 3_3:-69869725 Msa0468260:three_prime_UTR
TCCATTCCAGTCAGAACTTC+GGG 0.313058 3_3:+69871204 None:intergenic
GGAGGGTATGACTCCACTTT+AGG 0.326168 3_3:-69871361 Msa0468260:CDS
TGTAATGAGTGCACACATTC+TGG 0.330211 3_3:+69870087 None:intergenic
TTTAAGAAGAAACTGCATTC+AGG 0.330498 3_3:+69870309 None:intergenic
ACCTGGTTTCTTCTCCAAAC+AGG 0.348538 3_3:+69872694 None:intergenic
CATCATTGTAGTAGTTGGTT+TGG 0.351046 3_3:+69873446 None:intergenic
TGCACTCATTACAACCGATA+TGG 0.357948 3_3:-69870077 Msa0468260:CDS
AAAGAACAGTCTTCAGCTTC+AGG 0.362830 3_3:+69872769 None:intergenic
TAAGCATTCGTTTCCATAGC+TGG 0.369326 3_3:+69870387 None:intergenic
CAAATTTGCAAATTCCATAT+CGG 0.369456 3_3:+69870063 None:intergenic
TCAACAATCTCAATCACATT+TGG 0.369732 3_3:-69873256 Msa0468260:CDS
TGACAGTATTATTCAAGATC+TGG 0.369833 3_3:-69871701 Msa0468260:intron
GCAAGTTTAATCACCCTGTT+TGG 0.377149 3_3:-69872708 Msa0468260:CDS
ACTAAAGTTCAACATTTCAT+TGG 0.377225 3_3:+69869603 None:intergenic
GTGATTATGTGGTGAGTGAT+TGG 0.386277 3_3:-69872864 Msa0468260:CDS
TATGACTCCACTTTAGGATA+TGG 0.386430 3_3:-69871355 Msa0468260:CDS
TTCTGACTGGAATGGATATC+AGG 0.390808 3_3:-69871197 Msa0468260:intron
GAGGATGGAATTGAGGAAAA+AGG 0.391224 3_3:-69873114 Msa0468260:CDS
TTGTTTAGTTTACTTGAAAC+AGG 0.394761 3_3:+69869507 None:intergenic
TGCTCCAAACTTACAAGAAC+CGG 0.396802 3_3:+69871420 None:intergenic
TATCTCTCTTGGATTAGTAT+TGG 0.397975 3_3:-69869691 Msa0468260:three_prime_UTR
TGTTACATCATTGTAGTAGT+TGG 0.398909 3_3:+69873441 None:intergenic
CCTTGGCCTACCTATTCGTT+TGG 0.402338 3_3:-69871597 Msa0468260:intron
GATAACGTCCAGGCATAGTT+GGG 0.405460 3_3:+69870000 None:intergenic
TGCTGTTCTAGAATTTGATA+AGG 0.409379 3_3:-69873064 Msa0468260:CDS
ATTACTTTATTTAACTTCAC+TGG 0.413927 3_3:-69869812 Msa0468260:three_prime_UTR
TGATTGAGATATGAGGGTTC+AGG 0.415836 3_3:+69873186 None:intergenic
TGAGGGTTCAGGATGATGAT+TGG 0.419375 3_3:+69873197 None:intergenic
TCAGGATGATGATTGGAAAC+AGG 0.426300 3_3:+69873204 None:intergenic
CTTTCTGGAAACTCTTCAAC+TGG 0.426995 3_3:+69870342 None:intergenic
GCTGGATTGTTCACGGCATA+TGG 0.429292 3_3:+69870405 None:intergenic
TTCCATAGCTGGATTGTTCA+CGG 0.431515 3_3:+69870398 None:intergenic
GCAACAATTTAATAGCTTTG+AGG 0.433569 3_3:-69869915 Msa0468260:exon
AAAGCTTATACCAAACGAAT+AGG 0.433676 3_3:+69871587 None:intergenic
AAAGGTTCAAACTTTGAAGT+TGG 0.439652 3_3:-69873096 Msa0468260:CDS
ATCTGCTGAAAACTTTCCTC+TGG 0.440653 3_3:+69871640 None:intergenic
AGTGAGAGAAATAAGGTTGT+GGG 0.440988 3_3:+69873358 None:intergenic
GGTCAATTGATTGAGATATG+AGG 0.446208 3_3:+69873179 None:intergenic
CAAAAGGCTTGTGCCACATC+AGG 0.446756 3_3:+69869784 None:intergenic
AATTTCATCATCCAAAGAAC+CGG 0.447322 3_3:-69870254 Msa0468260:CDS
GATTGTTGCGATGGCATGGC+AGG 0.456960 3_3:+69873503 None:intergenic
GTGAGTGGAATTGATGATAA+AGG 0.457463 3_3:-69872979 Msa0468260:CDS
AAGGCAGACCCTTGTCACTT+AGG 0.458239 3_3:+69870208 None:intergenic
ATCATAATTTCACCGACTCC+AGG 0.458700 3_3:-69871235 Msa0468260:CDS
TCTTCAGCTTCAGGCCTCAA+TGG 0.459779 3_3:+69872778 None:intergenic
GAAAGATGAGGATGGAATTG+AGG 0.460065 3_3:-69873121 Msa0468260:CDS
GAAATTATGATTGGCGCAAA+AGG 0.463542 3_3:+69871247 None:intergenic
GCTGTTCTAGAATTTGATAA+GGG 0.467346 3_3:-69873063 Msa0468260:CDS
ATATATAGCTATTCCGCAAA+AGG 0.469443 3_3:+69869768 None:intergenic
CCCTTGTCACTTAGGTTGCA+TGG 0.470112 3_3:+69870216 None:intergenic
GACAGTATTATTCAAGATCT+GGG 0.475323 3_3:-69871700 Msa0468260:intron
CATCAAGTTCATGATGATGA+TGG 0.477201 3_3:-69872895 Msa0468260:CDS
GAACTTCGGGGAGAAGCCCC+TGG 0.478122 3_3:+69871217 None:intergenic
AACCGGTGCGCATGTAATAA+GGG 0.486519 3_3:+69871437 None:intergenic
GAGTGAGAGAAATAAGGTTG+TGG 0.488683 3_3:+69873357 None:intergenic
TCTGAAGCTCATCATCAAGT+TGG 0.490964 3_3:+69873157 None:intergenic
CCCGATCCTACATCTGTTGG+AGG 0.492082 3_3:-69871379 Msa0468260:CDS
CTGAAGCTCATCATCAAGTT+GGG 0.493894 3_3:+69873158 None:intergenic
AGTATTGAGGAGGCTGGAAG+TGG 0.494418 3_3:-69869951 Msa0468260:CDS
CATCCCGATCCTACATCTGT+TGG 0.500007 3_3:-69871382 Msa0468260:CDS
ATCCAAGAGAGATAACAAAC+TGG 0.502672 3_3:+69869700 None:intergenic
ATCCATTCCAGTCAGAACTT+CGG 0.504723 3_3:+69871203 None:intergenic
CCCTGTTTGGAGAAGAAACC+AGG 0.508022 3_3:-69872695 Msa0468260:intron
GGATTTAAATTTGCAATCCC+CGG 0.510053 3_3:+69870284 None:intergenic
GTCAATTGATTGAGATATGA+GGG 0.510110 3_3:+69873180 None:intergenic
TGATAACGTCCAGGCATAGT+TGG 0.516040 3_3:+69869999 None:intergenic
CACAAACTCGGCTAGTATTG+AGG 0.521153 3_3:-69869964 Msa0468260:CDS
CTTCTCCCCGAAGTTCTGAC+TGG 0.521361 3_3:-69871210 Msa0468260:CDS
CCCTCCAACAGATGTAGGAT+CGG 0.524928 3_3:+69871378 None:intergenic
TCCATTACCATATCCTAAAG+TGG 0.526454 3_3:+69871348 None:intergenic
TAGATGATTGTGACACGTCT+TGG 0.526550 3_3:+69871311 None:intergenic
TGATGAGCTTCAGAACAAGT+TGG 0.527576 3_3:-69873148 Msa0468260:CDS
GGAGTCGGTGAAATTATGAT+TGG 0.532435 3_3:+69871238 None:intergenic
TGGATGATGAAATTTGCAAT+GGG 0.532458 3_3:+69870263 None:intergenic
TCGGCTAGTATTGAGGAGGC+TGG 0.532916 3_3:-69869957 Msa0468260:CDS
ATTGGATTATATAGTTGTGC+TGG 0.534752 3_3:-69869673 Msa0468260:three_prime_UTR
AGGAGAAAGAGATGAATTGG+AGG 0.535415 3_3:-69872436 Msa0468260:CDS
TTGGATTTGAAAGATGAGGA+TGG 0.538154 3_3:-69873129 Msa0468260:CDS
CCTCCAACAGATGTAGGATC+GGG 0.539485 3_3:+69871379 None:intergenic
TCATAATTTCACCGACTCCA+GGG 0.539855 3_3:-69871234 Msa0468260:CDS
CAAGTTGGATTTGAAAGATG+AGG 0.539987 3_3:-69873133 Msa0468260:CDS
TTGGATGATGAAATTTGCAA+TGG 0.544786 3_3:+69870262 None:intergenic
CGGGGAGAAGCCCCTGGAGT+CGG 0.550896 3_3:+69871223 None:intergenic
CAGGATGATGATTGGAAACA+GGG 0.550924 3_3:+69873205 None:intergenic
TGCCGTGAACAATCCAGCTA+TGG 0.554540 3_3:-69870400 Msa0468260:CDS
TCATACCCTCCAACAGATGT+AGG 0.560380 3_3:+69871373 None:intergenic
CCCCGAAGTTCTGACTGGAA+TGG 0.564246 3_3:-69871205 Msa0468260:CDS
ATCAAGCCAGATAGAATGCA+AGG 0.564984 3_3:-69872409 Msa0468260:intron
TGCCCTTATTACATGCGCAC+CGG 0.565218 3_3:-69871439 Msa0468260:CDS
GGGGCGGTAGGTTTATCGGG+TGG 0.565245 3_3:+69870021 None:intergenic
ATTGAGGAGGCTGGAAGTGG+AGG 0.565408 3_3:-69869948 Msa0468260:CDS
TCCACTTTAGGATATGGTAA+TGG 0.572990 3_3:-69871349 Msa0468260:CDS
ACAATCCTCTCACACAAACT+CGG 0.577005 3_3:-69869976 Msa0468260:CDS
TGGTTGGAGTGGTGATTATG+TGG 0.578479 3_3:-69872875 Msa0468260:CDS
CTTATACCAAACGAATAGGT+AGG 0.578718 3_3:+69871591 None:intergenic
AAGATAATCTACAGCTGCTG+AGG 0.580481 3_3:+69869729 None:intergenic
ATCTGCCACAGTATGTGAGA+AGG 0.580537 3_3:-69872929 Msa0468260:CDS
CCTGGTTTCTTCTCCAAACA+GGG 0.582322 3_3:+69872695 None:intergenic
GATTGTTGAGACGGTTCCAT+TGG 0.582618 3_3:+69873270 None:intergenic
ACTAGCCGAGTTTGTGTGAG+AGG 0.582786 3_3:+69869971 None:intergenic
TGAAAATCACAAAATATGGA+TGG 0.582977 3_3:+69869846 None:intergenic
TGAGACGGTTCCATTGGTGA+TGG 0.584359 3_3:+69873276 None:intergenic
CATAATTTCACCGACTCCAG+GGG 0.587604 3_3:-69871233 Msa0468260:CDS
AAATAACTTACAGGTCTCAA+AGG 0.588071 3_3:+69870189 None:intergenic
AAACTGCATTCAGGTTCACC+AGG 0.589001 3_3:+69870318 None:intergenic
GAGTGATTGGATTGAGAATG+AGG 0.592997 3_3:-69872851 Msa0468260:CDS
TGGAGGGATTCCATCACCAA+TGG 0.596967 3_3:-69873286 Msa0468260:exon
GAGGATTGTTGATAACGTCC+AGG 0.597566 3_3:+69869990 None:intergenic
TGTGATTGAGATTGTTGAGA+CGG 0.598910 3_3:+69873261 None:intergenic
ACAGTATGTGAGAAGGGACA+AGG 0.601001 3_3:-69872922 Msa0468260:CDS
GGGTGGTCATACTTACAAGC+TGG 0.604093 3_3:+69870038 None:intergenic
ACAGTATTATTCAAGATCTG+GGG 0.604261 3_3:-69871699 Msa0468260:intron
TCTGCCACAGTATGTGAGAA+GGG 0.604844 3_3:-69872928 Msa0468260:CDS
GATGATGTGGATTGTTGCGA+TGG 0.613098 3_3:+69873494 None:intergenic
CAGTATTATTCAAGATCTGG+GGG 0.613736 3_3:-69871698 Msa0468260:CDS
GATTAATTAATGCGATGATG+TGG 0.613900 3_3:+69873481 None:intergenic
CCAAACGAATAGGTAGGCCA+AGG 0.617276 3_3:+69871597 None:intergenic
ATAACGTCCAGGCATAGTTG+GGG 0.618044 3_3:+69870001 None:intergenic
TATGTTGATGAAAGAAGCAA+TGG 0.625149 3_3:-69872817 Msa0468260:CDS
TGTGGATTGTTGCGATGGCA+TGG 0.625366 3_3:+69873499 None:intergenic
ATTGTTGCGATGGCATGGCA+GGG 0.625670 3_3:+69873504 None:intergenic
AAGTTCATGATGATGATGGT+TGG 0.628126 3_3:-69872891 Msa0468260:CDS
TTCACTGGTTATGCCTGATG+TGG 0.629637 3_3:-69869797 Msa0468260:three_prime_UTR
ATTGAGAATGAGGTGGTGAG+TGG 0.632045 3_3:-69872841 Msa0468260:CDS
TGTAAATTTGATCACACCAG+AGG 0.632238 3_3:-69871656 Msa0468260:CDS
TTCACGGCATATGGTGAAGG+TGG 0.632618 3_3:+69870414 None:intergenic
TCCATGCAACCTAAGTGACA+AGG 0.634773 3_3:-69870217 Msa0468260:CDS
GTTGATGAAAGAAGCAATGG+TGG 0.638205 3_3:-69872814 Msa0468260:CDS
CCGATCCTACATCTGTTGGA+GGG 0.641989 3_3:-69871378 Msa0468260:CDS
CAACTGGTGTATGCTTATGA+CGG 0.648143 3_3:+69870358 None:intergenic
TAACGTCCAGGCATAGTTGG+GGG 0.648794 3_3:+69870002 None:intergenic
TTGTTCACGGCATATGGTGA+AGG 0.648890 3_3:+69870411 None:intergenic
TTGTCCCTTCTCACATACTG+TGG 0.652329 3_3:+69872924 None:intergenic
GAAGAGTTTCCAGAAAGACC+TGG 0.657339 3_3:-69870336 Msa0468260:CDS
ACTGATGTTGATGAAGTGAG+TGG 0.661166 3_3:-69872994 Msa0468260:CDS
TGATGAGAATGTGAATGAGA+AGG 0.672400 3_3:-69873022 Msa0468260:CDS
CCATGCAACCTAAGTGACAA+GGG 0.676957 3_3:-69870216 Msa0468260:CDS
CATGATGATGATGGTTGGAG+TGG 0.678716 3_3:-69872886 Msa0468260:CDS
CAGTATGTGAGAAGGGACAA+GGG 0.679158 3_3:-69872921 Msa0468260:CDS
GAGCTGAACAGTATCCATTG+AGG 0.685815 3_3:-69872792 Msa0468260:CDS
AGGATGATGATTGGAAACAG+GGG 0.702661 3_3:+69873206 None:intergenic
AAACTCGGCTAGTATTGAGG+AGG 0.710931 3_3:-69869961 Msa0468260:CDS
TTGTCACTTAGGTTGCATGG+AGG 0.747188 3_3:+69870219 None:intergenic
CCATTCCAGTCAGAACTTCG+GGG 0.756262 3_3:+69871205 None:intergenic
TGATTGGATTGAGAATGAGG+TGG 0.762353 3_3:-69872848 Msa0468260:CDS
TTTCTGGTAGAAGACCATGG+GGG 0.864818 3_3:+69871286 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTTGAGGAAAATAAA+AGG + chr3_3:69869653-69869672 None:intergenic 15.0%
!! TTACTCATTATTATTATTTG+TGG - chr3_3:69870394-69870413 Msa0468260:CDS 15.0%
!!! AAACTTTTAAAACTAAATGA+AGG - chr3_3:69870556-69870575 Msa0468260:intron 15.0%
!!! TGTACATTTTTTTATATCAT+CGG - chr3_3:69871895-69871914 Msa0468260:intron 15.0%
!!! ATTTTCTTGTTTATGTATTA+TGG - chr3_3:69873402-69873421 Msa0468260:five_prime_UTR 15.0%
!! TCAATCAAATTTCAACTATT+AGG - chr3_3:69869706-69869725 Msa0468260:three_prime_UTR 20.0%
!! CAATCAAATTTCAACTATTA+GGG - chr3_3:69869707-69869726 Msa0468260:three_prime_UTR 20.0%
!! TGCATTCATTTATGTATATT+AGG - chr3_3:69870440-69870459 Msa0468260:CDS 20.0%
!!! TTTTTCTGACATAATTTACT+CGG + chr3_3:69871061-69871080 None:intergenic 20.0%
!!! TTTGCTATATTGAAAAATGT+TGG - chr3_3:69871168-69871187 Msa0468260:intron 20.0%
!! GTTATAATCTAAATAATAGC+AGG + chr3_3:69871278-69871297 None:intergenic 20.0%
!!! AAACTTTTTTCTCTCTTTTT+AGG - chr3_3:69872096-69872115 Msa0468260:intron 20.0%
!! AAATGCAAGTAATATACTTA+AGG - chr3_3:69872343-69872362 Msa0468260:intron 20.0%
!! TCATAAGAATAATTAACTGA+CGG + chr3_3:69872505-69872524 None:intergenic 20.0%
!! AAGATGAAAATCACAAAATA+TGG + chr3_3:69873192-69873211 None:intergenic 20.0%
!!! ATTACTTTATTTAACTTCAC+TGG - chr3_3:69873219-69873238 Msa0468260:CDS 20.0%
!! TGTTCTTTTTATCTTAAGAC+TGG - chr3_3:69870277-69870296 Msa0468260:CDS 25.0%
! TCTTATATGTTATAGCATAG+CGG - chr3_3:69870884-69870903 Msa0468260:intron 25.0%
!!! TTTTTCAATATAGCAAAGCT+AGG + chr3_3:69871165-69871184 None:intergenic 25.0%
! TACTGTCATATCATAACATT+AGG + chr3_3:69871318-69871337 None:intergenic 25.0%
! GACATTGTATGATAGAAAAT+TGG + chr3_3:69871943-69871962 None:intergenic 25.0%
! GTATAGTTAATACTGAGTTA+TGG - chr3_3:69872020-69872039 Msa0468260:intron 25.0%
! GTCAGTTAATTATTCTTATG+AGG - chr3_3:69872504-69872523 Msa0468260:intron 25.0%
!! GACCAATTTGTATTTTGTAT+AGG - chr3_3:69872562-69872581 Msa0468260:intron 25.0%
! TGCCTATACAAAATACAAAT+TGG + chr3_3:69872567-69872586 None:intergenic 25.0%
! ATCAGAGTAAAATAACTTAC+AGG + chr3_3:69872854-69872873 None:intergenic 25.0%
! AAATGTTAGATCAAAGAAAC+TGG + chr3_3:69872903-69872922 None:intergenic 25.0%
! TTTAATATCTCGTGAAATTC+AGG - chr3_3:69872920-69872939 Msa0468260:CDS 25.0%
! TTAATATCTCGTGAAATTCA+GGG - chr3_3:69872921-69872940 Msa0468260:CDS 25.0%
! CAAATTTGCAAATTCCATAT+CGG + chr3_3:69872971-69872990 None:intergenic 25.0%
!! GATTGAAACATTTTCTATCA+AGG - chr3_3:69873138-69873157 Msa0468260:CDS 25.0%
!! ATTTTCTATCAAGGATCTTA+TGG - chr3_3:69873147-69873166 Msa0468260:CDS 25.0%
! TGAAAATCACAAAATATGGA+TGG + chr3_3:69873188-69873207 None:intergenic 25.0%
! ACTAAAGTTCAACATTTCAT+TGG + chr3_3:69873431-69873450 None:intergenic 25.0%
!! TTTTCCAAAAATCTCTTAAC+AGG + chr3_3:69873494-69873513 None:intergenic 25.0%
!!! AAGAGATTTTTGGAAAAATC+AGG - chr3_3:69873497-69873516 Msa0468260:five_prime_UTR 25.0%
!! TTGTTTAGTTTACTTGAAAC+AGG + chr3_3:69873527-69873546 None:intergenic 25.0%
GATTAATTAATGCGATGATG+TGG + chr3_3:69869553-69869572 None:intergenic 30.0%
TGTTACATCATTGTAGTAGT+TGG + chr3_3:69869593-69869612 None:intergenic 30.0%
TTGAAAGAGTGAGAGAAATA+AGG + chr3_3:69869683-69869702 None:intergenic 30.0%
!!! TAGGGTTTCTTTTTGAAATG+TGG - chr3_3:69869725-69869744 Msa0468260:three_prime_UTR 30.0%
!!! GTTTCTTTTTGAAATGTGGA+GGG - chr3_3:69869729-69869748 Msa0468260:three_prime_UTR 30.0%
TCAACAATCTCAATCACATT+TGG - chr3_3:69869775-69869794 Msa0468260:three_prime_UTR 30.0%
GTCAATTGATTGAGATATGA+GGG + chr3_3:69869854-69869873 None:intergenic 30.0%
!! AAAGGTTCAAACTTTGAAGT+TGG - chr3_3:69869935-69869954 Msa0468260:CDS 30.0%
!! TGCTGTTCTAGAATTTGATA+AGG - chr3_3:69869967-69869986 Msa0468260:CDS 30.0%
!! GCTGTTCTAGAATTTGATAA+GGG - chr3_3:69869968-69869987 Msa0468260:CDS 30.0%
! TATGTTGATGAAAGAAGCAA+TGG - chr3_3:69870214-69870233 Msa0468260:CDS 30.0%
!! AAATGAAGGTTTTTAGAGAG+AGG - chr3_3:69870570-69870589 Msa0468260:intron 30.0%
!! AATGAAGGTTTTTAGAGAGA+GGG - chr3_3:69870571-69870590 Msa0468260:intron 30.0%
AAAATACCTTGCATTCTATC+TGG + chr3_3:69870631-69870650 None:intergenic 30.0%
! TTTTATGAATGCCTTGACTA+TGG - chr3_3:69870700-69870719 Msa0468260:intron 30.0%
TGTTGTCAAATAGACTATAG+CGG - chr3_3:69870954-69870973 Msa0468260:intron 30.0%
ATCAATTATGAATATGCTGC+TGG - chr3_3:69871104-69871123 Msa0468260:intron 30.0%
TGACAGTATTATTCAAGATC+TGG - chr3_3:69871330-69871349 Msa0468260:CDS 30.0%
GACAGTATTATTCAAGATCT+GGG - chr3_3:69871331-69871350 Msa0468260:CDS 30.0%
ACAGTATTATTCAAGATCTG+GGG - chr3_3:69871332-69871351 Msa0468260:CDS 30.0%
AAAGCTTATACCAAACGAAT+AGG + chr3_3:69871447-69871466 None:intergenic 30.0%
! AGCAGTTTCATTGAATTTTC+TGG + chr3_3:69871764-69871783 None:intergenic 30.0%
!! TGACTTTTAAGTAACTGATG+TGG + chr3_3:69872077-69872096 None:intergenic 30.0%
! GTACTTAATCTGAAATGATG+TGG + chr3_3:69872164-69872183 None:intergenic 30.0%
CATTTCAGATTAAGTACCTT+TGG - chr3_3:69872167-69872186 Msa0468260:intron 30.0%
! ATTTGTTAGTAATTGCCCTT+AGG - chr3_3:69872226-69872245 Msa0468260:intron 30.0%
TTTGTTAGTAATTGCCCTTA+GGG - chr3_3:69872227-69872246 Msa0468260:intron 30.0%
CAAAACAACGAATAATAGAG+AGG + chr3_3:69872444-69872463 None:intergenic 30.0%
AGAATAATTAACTGACGGAA+AGG + chr3_3:69872500-69872519 None:intergenic 30.0%
GTTAATTATTCTTATGAGGC+AGG - chr3_3:69872508-69872527 Msa0468260:intron 30.0%
TACAAATTGGTCAAATTGCT+GGG + chr3_3:69872554-69872573 None:intergenic 30.0%
ATACAAATTGGTCAAATTGC+TGG + chr3_3:69872555-69872574 None:intergenic 30.0%
TTTAAGAAGAAACTGCATTC+AGG + chr3_3:69872725-69872744 None:intergenic 30.0%
TGCAGTTTCTTCTTAAAAAC+CGG - chr3_3:69872728-69872747 Msa0468260:CDS 30.0%
TGGATGATGAAATTTGCAAT+GGG + chr3_3:69872771-69872790 None:intergenic 30.0%
TTGGATGATGAAATTTGCAA+TGG + chr3_3:69872772-69872791 None:intergenic 30.0%
AATTTCATCATCCAAAGAAC+CGG - chr3_3:69872777-69872796 Msa0468260:CDS 30.0%
! AATTTTGTAATCCGGTTCTT+TGG + chr3_3:69872791-69872810 None:intergenic 30.0%
! ATGGAGGTAATTTTGTAATC+CGG + chr3_3:69872799-69872818 None:intergenic 30.0%
AAATAACTTACAGGTCTCAA+AGG + chr3_3:69872845-69872864 None:intergenic 30.0%
CGATATGGAATTTGCAAATT+TGG - chr3_3:69872969-69872988 Msa0468260:CDS 30.0%
! GCAACAATTTAATAGCTTTG+AGG - chr3_3:69873116-69873135 Msa0468260:CDS 30.0%
ATATATAGCTATTCCGCAAA+AGG + chr3_3:69873266-69873285 None:intergenic 30.0%
!! TATCTCTCTTGGATTAGTAT+TGG - chr3_3:69873340-69873359 Msa0468260:five_prime_UTR 30.0%
ATTGGATTATATAGTTGTGC+TGG - chr3_3:69873358-69873377 Msa0468260:five_prime_UTR 30.0%
CAAAAGAACTGCTCAATTAA+TGG - chr3_3:69873456-69873475 Msa0468260:five_prime_UTR 30.0%
CATCATTGTAGTAGTTGGTT+TGG + chr3_3:69869588-69869607 None:intergenic 35.0%
!!! AAGGTTGTGGGTTTTATTTG+AGG + chr3_3:69869664-69869683 None:intergenic 35.0%
AGTGAGAGAAATAAGGTTGT+GGG + chr3_3:69869676-69869695 None:intergenic 35.0%
!!! GGTTTCTTTTTGAAATGTGG+AGG - chr3_3:69869728-69869747 Msa0468260:three_prime_UTR 35.0%
TGTGATTGAGATTGTTGAGA+CGG + chr3_3:69869773-69869792 None:intergenic 35.0%
GGTCAATTGATTGAGATATG+AGG + chr3_3:69869855-69869874 None:intergenic 35.0%
! CAAGTTGGATTTGAAAGATG+AGG - chr3_3:69869898-69869917 Msa0468260:three_prime_UTR 35.0%
TTGGATTTGAAAGATGAGGA+TGG - chr3_3:69869902-69869921 Msa0468260:three_prime_UTR 35.0%
TGATGAGAATGTGAATGAGA+AGG - chr3_3:69870009-69870028 Msa0468260:CDS 35.0%
! GTGAGTGGAATTGATGATAA+AGG - chr3_3:69870052-69870071 Msa0468260:CDS 35.0%
! CATCAAGTTCATGATGATGA+TGG - chr3_3:69870136-69870155 Msa0468260:intron 35.0%
!! AAGTTCATGATGATGATGGT+TGG - chr3_3:69870140-69870159 Msa0468260:intron 35.0%
AAGACTGGAACTTGCAAATT+TGG - chr3_3:69870292-69870311 Msa0468260:CDS 35.0%
GAACATAAGAAAGTGATACC+TGG + chr3_3:69870357-69870376 None:intergenic 35.0%
! AGTAAGAGTGTACTAGAAAG+AGG + chr3_3:69870379-69870398 None:intergenic 35.0%
GATTGTGTAAGGTTAACTCT+AGG - chr3_3:69870789-69870808 Msa0468260:intron 35.0%
AGCTCAGTATTGTCAAATAG+CGG - chr3_3:69870850-69870869 Msa0468260:intron 35.0%
TATTGTCAAATAGCGGCTAT+AGG - chr3_3:69870857-69870876 Msa0468260:intron 35.0%
ATTGTCAAATAGCGGCTATA+GGG - chr3_3:69870858-69870877 Msa0468260:intron 35.0%
TTGTCAATCACGGAAAATAG+TGG + chr3_3:69871016-69871035 None:intergenic 35.0%
ATAGGTAGTGTTGTCAATCA+CGG + chr3_3:69871026-69871045 None:intergenic 35.0%
TGAATATGCTGCTGGAATAA+TGG - chr3_3:69871112-69871131 Msa0468260:intron 35.0%
GAATATGCTGCTGGAATAAT+GGG - chr3_3:69871113-69871132 Msa0468260:intron 35.0%
AAACTTTCGTTGTTGTATCC+TGG - chr3_3:69871139-69871158 Msa0468260:intron 35.0%
ACAAGTTTAAGAAATGGCTC+AGG + chr3_3:69871255-69871274 None:intergenic 35.0%
AGCAGGACAAGTTTAAGAAA+TGG + chr3_3:69871261-69871280 None:intergenic 35.0%
CAGTATTATTCAAGATCTGG+GGG - chr3_3:69871333-69871352 Msa0468260:CDS 35.0%
TGTAAATTTGATCACACCAG+AGG - chr3_3:69871375-69871394 Msa0468260:CDS 35.0%
! GTTTTACAGCTTAACTTCCT+TGG - chr3_3:69871417-69871436 Msa0468260:CDS 35.0%
CTTATACCAAACGAATAGGT+AGG + chr3_3:69871443-69871462 None:intergenic 35.0%
!! TATGACTCCACTTTAGGATA+TGG - chr3_3:69871676-69871695 Msa0468260:CDS 35.0%
!! TCCACTTTAGGATATGGTAA+TGG - chr3_3:69871682-69871701 Msa0468260:CDS 35.0%
TCCATTACCATATCCTAAAG+TGG + chr3_3:69871686-69871705 None:intergenic 35.0%
!! ATTTTCTGGTAGAAGACCAT+GGG + chr3_3:69871750-69871769 None:intergenic 35.0%
!! AATTTTCTGGTAGAAGACCA+TGG + chr3_3:69871751-69871770 None:intergenic 35.0%
GAAATTATGATTGGCGCAAA+AGG + chr3_3:69871787-69871806 None:intergenic 35.0%
TACAATGTCAATGTTTCCTG+TGG - chr3_3:69871954-69871973 Msa0468260:intron 35.0%
ATTATTGACAACTGTGGACT+AGG + chr3_3:69871980-69871999 None:intergenic 35.0%
TTACACGTCAAACTATCACT+TGG + chr3_3:69872385-69872404 None:intergenic 35.0%
! AACGAATAATAGAGAGGTGT+AGG + chr3_3:69872438-69872457 None:intergenic 35.0%
!! CACCTTTCTATTTTTCTGAG+AGG - chr3_3:69872585-69872604 Msa0468260:intron 35.0%
CTCCTCTCAGAAAAATAGAA+AGG + chr3_3:69872590-69872609 None:intergenic 35.0%
GCAGTTTCTTCTTAAAAACC+GGG - chr3_3:69872729-69872748 Msa0468260:CDS 35.0%
CAGTTTCTTCTTAAAAACCG+GGG - chr3_3:69872730-69872749 Msa0468260:CDS 35.0%
GGATTTAAATTTGCAATCCC+CGG + chr3_3:69872750-69872769 None:intergenic 35.0%
! AGCAGCTGTAGATTATCTTT+TGG - chr3_3:69873306-69873325 Msa0468260:five_prime_UTR 35.0%
!! TTCCAGTTTGTTATCTCTCT+TGG - chr3_3:69873329-69873348 Msa0468260:five_prime_UTR 35.0%
ATCCAAGAGAGATAACAAAC+TGG + chr3_3:69873334-69873353 None:intergenic 35.0%
! GTAGCCTGTTAAGAGATTTT+TGG - chr3_3:69873487-69873506 Msa0468260:five_prime_UTR 35.0%
GAGTGAGAGAAATAAGGTTG+TGG + chr3_3:69869677-69869696 None:intergenic 40.0%
AGGATGATGATTGGAAACAG+GGG + chr3_3:69869828-69869847 None:intergenic 40.0%
CAGGATGATGATTGGAAACA+GGG + chr3_3:69869829-69869848 None:intergenic 40.0%
TCAGGATGATGATTGGAAAC+AGG + chr3_3:69869830-69869849 None:intergenic 40.0%
TGATTGAGATATGAGGGTTC+AGG + chr3_3:69869848-69869867 None:intergenic 40.0%
CTGAAGCTCATCATCAAGTT+GGG + chr3_3:69869876-69869895 None:intergenic 40.0%
! TCTGAAGCTCATCATCAAGT+TGG + chr3_3:69869877-69869896 None:intergenic 40.0%
TGATGAGCTTCAGAACAAGT+TGG - chr3_3:69869883-69869902 Msa0468260:three_prime_UTR 40.0%
GAAAGATGAGGATGGAATTG+AGG - chr3_3:69869910-69869929 Msa0468260:exon 40.0%
GAGGATGGAATTGAGGAAAA+AGG - chr3_3:69869917-69869936 Msa0468260:exon 40.0%
! ACTGATGTTGATGAAGTGAG+TGG - chr3_3:69870037-69870056 Msa0468260:CDS 40.0%
GTGATTATGTGGTGAGTGAT+TGG - chr3_3:69870167-69870186 Msa0468260:intron 40.0%
GAGTGATTGGATTGAGAATG+AGG - chr3_3:69870180-69870199 Msa0468260:intron 40.0%
TGATTGGATTGAGAATGAGG+TGG - chr3_3:69870183-69870202 Msa0468260:intron 40.0%
! GTTGATGAAAGAAGCAATGG+TGG - chr3_3:69870217-69870236 Msa0468260:CDS 40.0%
AAAGAACAGTCTTCAGCTTC+AGG + chr3_3:69870265-69870284 None:intergenic 40.0%
GCAAGTTTAATCACCCTGTT+TGG - chr3_3:69870323-69870342 Msa0468260:CDS 40.0%
AGGAGAAAGAGATGAATTGG+AGG - chr3_3:69870595-69870614 Msa0468260:intron 40.0%
ATCAAGCCAGATAGAATGCA+AGG - chr3_3:69870622-69870641 Msa0468260:intron 40.0%
TTTCCATGGGAGATTGTGTA+AGG - chr3_3:69870778-69870797 Msa0468260:intron 40.0%
TAACCTTACACAATCTCCCA+TGG + chr3_3:69870784-69870803 None:intergenic 40.0%
TGTGTAAGGTTAACTCTAGG+AGG - chr3_3:69870792-69870811 Msa0468260:intron 40.0%
TTGTCAAATAGCGGCTATAG+GGG - chr3_3:69870859-69870878 Msa0468260:intron 40.0%
ATCTGCTGAAAACTTTCCTC+TGG + chr3_3:69871394-69871413 None:intergenic 40.0%
!! TCAGCATAGCTTGCTAAGTA+AGG + chr3_3:69871473-69871492 None:intergenic 40.0%
! CAGCTATCTTTTTCTGCTGT+CGG - chr3_3:69871514-69871533 Msa0468260:intron 40.0%
AACAACGCGGCTAAAATTTC+AGG + chr3_3:69871549-69871568 None:intergenic 40.0%
CCCTATCATGAAAAACAACG+CGG + chr3_3:69871562-69871581 None:intergenic 40.0%
!!! CCGCGTTGTTTTTCATGATA+GGG - chr3_3:69871559-69871578 Msa0468260:intron 40.0%
TGCTCCAAACTTACAAGAAC+CGG + chr3_3:69871614-69871633 None:intergenic 40.0%
TAGATGATTGTGACACGTCT+TGG + chr3_3:69871723-69871742 None:intergenic 40.0%
!! TTTTCTGGTAGAAGACCATG+GGG + chr3_3:69871749-69871768 None:intergenic 40.0%
GGAGTCGGTGAAATTATGAT+TGG + chr3_3:69871796-69871815 None:intergenic 40.0%
!! ATCATAATTTCACCGACTCC+AGG - chr3_3:69871796-69871815 Msa0468260:intron 40.0%
!! TCATAATTTCACCGACTCCA+GGG - chr3_3:69871797-69871816 Msa0468260:intron 40.0%
ATCCATTCCAGTCAGAACTT+CGG + chr3_3:69871831-69871850 None:intergenic 40.0%
TTCTGACTGGAATGGATATC+AGG - chr3_3:69871834-69871853 Msa0468260:intron 40.0%
CTGAGCATTATTGACAACTG+TGG + chr3_3:69871986-69872005 None:intergenic 40.0%
GAGTTATGGCATGATCTGTA+AGG - chr3_3:69872034-69872053 Msa0468260:intron 40.0%
!! TCTCTTTTTAGGCTCTCTTG+TGG - chr3_3:69872107-69872126 Msa0468260:intron 40.0%
GATGTGGTTGTGCAATAGAA+TGG + chr3_3:69872148-69872167 None:intergenic 40.0%
GTGGTGAAGGAAACAACAAT+TGG - chr3_3:69872252-69872271 Msa0468260:intron 40.0%
TGTTGCATGCCATTCTTATG+AGG - chr3_3:69872462-69872481 Msa0468260:intron 40.0%
AAATTGCTGGGAAAATGGCA+AGG + chr3_3:69872542-69872561 None:intergenic 40.0%
TGGTCAAATTGCTGGGAAAA+TGG + chr3_3:69872547-69872566 None:intergenic 40.0%
TTCCATAGCTGGATTGTTCA+CGG + chr3_3:69872636-69872655 None:intergenic 40.0%
TAAGCATTCGTTTCCATAGC+TGG + chr3_3:69872647-69872666 None:intergenic 40.0%
CAACTGGTGTATGCTTATGA+CGG + chr3_3:69872676-69872695 None:intergenic 40.0%
! CTTTCTGGAAACTCTTCAAC+TGG + chr3_3:69872692-69872711 None:intergenic 40.0%
! AACTGGTGACGTGTACATTA+GGG + chr3_3:69872886-69872905 None:intergenic 40.0%
! AAACTGGTGACGTGTACATT+AGG + chr3_3:69872887-69872906 None:intergenic 40.0%
TGTAATGAGTGCACACATTC+TGG + chr3_3:69872947-69872966 None:intergenic 40.0%
TGCACTCATTACAACCGATA+TGG - chr3_3:69872954-69872973 Msa0468260:CDS 40.0%
ACAATCCTCTCACACAAACT+CGG - chr3_3:69873055-69873074 Msa0468260:CDS 40.0%
AAGATAATCTACAGCTGCTG+AGG + chr3_3:69873305-69873324 None:intergenic 40.0%
! GATGATGTGGATTGTTGCGA+TGG + chr3_3:69869540-69869559 None:intergenic 45.0%
GATTGTTGAGACGGTTCCAT+TGG + chr3_3:69869764-69869783 None:intergenic 45.0%
TGAGGGTTCAGGATGATGAT+TGG + chr3_3:69869837-69869856 None:intergenic 45.0%
ATCTGCCACAGTATGTGAGA+AGG - chr3_3:69870102-69870121 Msa0468260:intron 45.0%
TCTGCCACAGTATGTGAGAA+GGG - chr3_3:69870103-69870122 Msa0468260:intron 45.0%
TTGTCCCTTCTCACATACTG+TGG + chr3_3:69870110-69870129 None:intergenic 45.0%
ACAGTATGTGAGAAGGGACA+AGG - chr3_3:69870109-69870128 Msa0468260:intron 45.0%
CAGTATGTGAGAAGGGACAA+GGG - chr3_3:69870110-69870129 Msa0468260:intron 45.0%
!! CATGATGATGATGGTTGGAG+TGG - chr3_3:69870145-69870164 Msa0468260:intron 45.0%
TGGTTGGAGTGGTGATTATG+TGG - chr3_3:69870156-69870175 Msa0468260:intron 45.0%
ATTGAGAATGAGGTGGTGAG+TGG - chr3_3:69870190-69870209 Msa0468260:intron 45.0%
GAGCTGAACAGTATCCATTG+AGG - chr3_3:69870239-69870258 Msa0468260:CDS 45.0%
CCTGGTTTCTTCTCCAAACA+GGG + chr3_3:69870339-69870358 None:intergenic 45.0%
ACCTGGTTTCTTCTCCAAAC+AGG + chr3_3:69870340-69870359 None:intergenic 45.0%
!! AAGGTTTTTAGAGAGAGGGC+AGG - chr3_3:69870575-69870594 Msa0468260:intron 45.0%
GGCAGGAGAAAGAGATGAAT+TGG - chr3_3:69870592-69870611 Msa0468260:intron 45.0%
ATCTCCCATGGAAAATCCCA+CGG + chr3_3:69870772-69870791 None:intergenic 45.0%
TGTCAAATAGCGGCTATAGG+GGG - chr3_3:69870860-69870879 Msa0468260:intron 45.0%
CAATATAGCAAAGCTAGGCC+AGG + chr3_3:69871160-69871179 None:intergenic 45.0%
AGATCTGGGGGTTGTAAGTT+TGG - chr3_3:69871345-69871364 Msa0468260:CDS 45.0%
TGGGGGTTGTAAGTTTGGAA+AGG - chr3_3:69871350-69871369 Msa0468260:CDS 45.0%
!!! GCCGCGTTGTTTTTCATGAT+AGG - chr3_3:69871558-69871577 Msa0468260:intron 45.0%
AACCGGTGCGCATGTAATAA+GGG + chr3_3:69871597-69871616 None:intergenic 45.0%
TCATACCCTCCAACAGATGT+AGG + chr3_3:69871661-69871680 None:intergenic 45.0%
! TTTCTGGTAGAAGACCATGG+GGG + chr3_3:69871748-69871767 None:intergenic 45.0%
!! CATAATTTCACCGACTCCAG+GGG - chr3_3:69871798-69871817 Msa0468260:intron 45.0%
TCCATTCCAGTCAGAACTTC+GGG + chr3_3:69871830-69871849 None:intergenic 45.0%
ACTTCTGAGCATGCATCCAA+AGG + chr3_3:69872186-69872205 None:intergenic 45.0%
AGTAATTGCCCTTAGGGTAG+TGG - chr3_3:69872233-69872252 Msa0468260:intron 45.0%
TTCCTTCACCACTACCCTAA+GGG + chr3_3:69872244-69872263 None:intergenic 45.0%
TTTCCTTCACCACTACCCTA+AGG + chr3_3:69872245-69872264 None:intergenic 45.0%
TAACTGACGGAAAGGAACAG+AGG + chr3_3:69872492-69872511 None:intergenic 45.0%
TTGTTCACGGCATATGGTGA+AGG + chr3_3:69872623-69872642 None:intergenic 45.0%
GAAGAGTTTCCAGAAAGACC+TGG - chr3_3:69872695-69872714 Msa0468260:intron 45.0%
! AAACTGCATTCAGGTTCACC+AGG + chr3_3:69872716-69872735 None:intergenic 45.0%
! TTGTCACTTAGGTTGCATGG+AGG + chr3_3:69872815-69872834 None:intergenic 45.0%
TCCATGCAACCTAAGTGACA+AGG - chr3_3:69872814-69872833 Msa0468260:CDS 45.0%
CCATGCAACCTAAGTGACAA+GGG - chr3_3:69872815-69872834 Msa0468260:CDS 45.0%
ATAACGTCCAGGCATAGTTG+GGG + chr3_3:69873033-69873052 None:intergenic 45.0%
GATAACGTCCAGGCATAGTT+GGG + chr3_3:69873034-69873053 None:intergenic 45.0%
! TGATAACGTCCAGGCATAGT+TGG + chr3_3:69873035-69873054 None:intergenic 45.0%
!! GAGGATTGTTGATAACGTCC+AGG + chr3_3:69873044-69873063 None:intergenic 45.0%
CACAAACTCGGCTAGTATTG+AGG - chr3_3:69873067-69873086 Msa0468260:CDS 45.0%
! AAACTCGGCTAGTATTGAGG+AGG - chr3_3:69873070-69873089 Msa0468260:CDS 45.0%
! TTCACTGGTTATGCCTGATG+TGG - chr3_3:69873234-69873253 Msa0468260:CDS 45.0%
ATTGTTGCGATGGCATGGCA+GGG + chr3_3:69869530-69869549 None:intergenic 50.0%
! TGTGGATTGTTGCGATGGCA+TGG + chr3_3:69869535-69869554 None:intergenic 50.0%
TGGAGGGATTCCATCACCAA+TGG - chr3_3:69869745-69869764 Msa0468260:three_prime_UTR 50.0%
TGAGACGGTTCCATTGGTGA+TGG + chr3_3:69869758-69869777 None:intergenic 50.0%
TCTTCAGCTTCAGGCCTCAA+TGG + chr3_3:69870256-69870275 None:intergenic 50.0%
CCCTGTTTGGAGAAGAAACC+AGG - chr3_3:69870336-69870355 Msa0468260:CDS 50.0%
AATGCAAATTCCCTTGCCCG+TGG - chr3_3:69870752-69870771 Msa0468260:intron 50.0%
ATGCAAATTCCCTTGCCCGT+GGG - chr3_3:69870753-69870772 Msa0468260:intron 50.0%
ATGGAAAATCCCACGGGCAA+GGG + chr3_3:69870765-69870784 None:intergenic 50.0%
! TTGCCCGTGGGATTTTCCAT+GGG - chr3_3:69870765-69870784 Msa0468260:intron 50.0%
TCTCCCATGGAAAATCCCAC+GGG + chr3_3:69870771-69870790 None:intergenic 50.0%
ACTCGGTCGAACTCAAGCAT+AGG + chr3_3:69871044-69871063 None:intergenic 50.0%
CCAAACGAATAGGTAGGCCA+AGG + chr3_3:69871437-69871456 None:intergenic 50.0%
CCTTGGCCTACCTATTCGTT+TGG - chr3_3:69871434-69871453 Msa0468260:CDS 50.0%
CGCGGCTAAAATTTCAGGAC+AGG + chr3_3:69871544-69871563 None:intergenic 50.0%
TGCCCTTATTACATGCGCAC+CGG - chr3_3:69871592-69871611 Msa0468260:intron 50.0%
GAACCGGTGCGCATGTAATA+AGG + chr3_3:69871598-69871617 None:intergenic 50.0%
!! CGCACCGGTTCTTGTAAGTT+TGG - chr3_3:69871607-69871626 Msa0468260:CDS 50.0%
CATCCCGATCCTACATCTGT+TGG - chr3_3:69871649-69871668 Msa0468260:CDS 50.0%
CCTCCAACAGATGTAGGATC+GGG + chr3_3:69871655-69871674 None:intergenic 50.0%
CCCTCCAACAGATGTAGGAT+CGG + chr3_3:69871656-69871675 None:intergenic 50.0%
CCGATCCTACATCTGTTGGA+GGG - chr3_3:69871653-69871672 Msa0468260:CDS 50.0%
! GGAGGGTATGACTCCACTTT+AGG - chr3_3:69871670-69871689 Msa0468260:CDS 50.0%
CACAATCATCTACACCCCCA+TGG - chr3_3:69871731-69871750 Msa0468260:intron 50.0%
CCATTCCAGTCAGAACTTCG+GGG + chr3_3:69871829-69871848 None:intergenic 50.0%
GCATGCCATTCTTATGAGGC+AGG - chr3_3:69872466-69872485 Msa0468260:intron 50.0%
AGAGGCCTGCCTCATAAGAA+TGG + chr3_3:69872474-69872493 None:intergenic 50.0%
GGGAAAATGGCAAGGAAGAG+AGG + chr3_3:69872534-69872553 None:intergenic 50.0%
TTCACGGCATATGGTGAAGG+TGG + chr3_3:69872620-69872639 None:intergenic 50.0%
GCTGGATTGTTCACGGCATA+TGG + chr3_3:69872629-69872648 None:intergenic 50.0%
TGCCGTGAACAATCCAGCTA+TGG - chr3_3:69872631-69872650 Msa0468260:intron 50.0%
TCAGGTTCACCAGGTCTTTC+TGG + chr3_3:69872707-69872726 None:intergenic 50.0%
! CCCTTGTCACTTAGGTTGCA+TGG + chr3_3:69872818-69872837 None:intergenic 50.0%
! AAGGCAGACCCTTGTCACTT+AGG + chr3_3:69872826-69872845 None:intergenic 50.0%
GGGTGGTCATACTTACAAGC+TGG + chr3_3:69872996-69873015 None:intergenic 50.0%
TAACGTCCAGGCATAGTTGG+GGG + chr3_3:69873032-69873051 None:intergenic 50.0%
ACTAGCCGAGTTTGTGTGAG+AGG + chr3_3:69873063-69873082 None:intergenic 50.0%
! AGTATTGAGGAGGCTGGAAG+TGG - chr3_3:69873080-69873099 Msa0468260:CDS 50.0%
CAAAAGGCTTGTGCCACATC+AGG + chr3_3:69873250-69873269 None:intergenic 50.0%
! GATGTGGCACAAGCCTTTTG+CGG - chr3_3:69873250-69873269 Msa0468260:CDS 50.0%
GATTGTTGCGATGGCATGGC+AGG + chr3_3:69869531-69869550 None:intergenic 55.0%
CGACGCACAAGCCATAGTCA+AGG + chr3_3:69870714-69870733 None:intergenic 55.0%
CATGGAAAATCCCACGGGCA+AGG + chr3_3:69870766-69870785 None:intergenic 55.0%
! CTTGCCCGTGGGATTTTCCA+TGG - chr3_3:69870764-69870783 Msa0468260:intron 55.0%
CCCGATCCTACATCTGTTGG+AGG - chr3_3:69871652-69871671 Msa0468260:CDS 55.0%
! CTTCTCCCCGAAGTTCTGAC+TGG - chr3_3:69871821-69871840 Msa0468260:intron 55.0%
! CCCCGAAGTTCTGACTGGAA+TGG - chr3_3:69871826-69871845 Msa0468260:intron 55.0%
!! ACAACTGTGGACTAGGCCAC+AGG + chr3_3:69871973-69871992 None:intergenic 55.0%
! TGCCCTTAGGGTAGTGGTGA+AGG - chr3_3:69872239-69872258 Msa0468260:intron 55.0%
TTGGGGGCGGTAGGTTTATC+GGG + chr3_3:69873016-69873035 None:intergenic 55.0%
GTTGGGGGCGGTAGGTTTAT+CGG + chr3_3:69873017-69873036 None:intergenic 55.0%
! TCGGCTAGTATTGAGGAGGC+TGG - chr3_3:69873074-69873093 Msa0468260:CDS 55.0%
ATTGAGGAGGCTGGAAGTGG+AGG - chr3_3:69873083-69873102 Msa0468260:CDS 55.0%
GAACTTCGGGGAGAAGCCCC+TGG + chr3_3:69871817-69871836 None:intergenic 65.0%
GGGGCGGTAGGTTTATCGGG+TGG + chr3_3:69873013-69873032 None:intergenic 65.0%
CAGGCATAGTTGGGGGCGGT+AGG + chr3_3:69873025-69873044 None:intergenic 65.0%
CTACCGCCCCCAACTATGCC+TGG - chr3_3:69873023-69873042 Msa0468260:CDS 65.0%
CGTCCAGGCATAGTTGGGGG+CGG + chr3_3:69873029-69873048 None:intergenic 65.0%
CGGGGAGAAGCCCCTGGAGT+CGG + chr3_3:69871811-69871830 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 69869489 69873564 69869489 ID=Msa0468260;Name=Msa0468260
chr3_3 mRNA 69869489 69873564 69869489 ID=Msa0468260-mRNA-1;Parent=Msa0468260;Name=Msa0468260-mRNA-1;_AED=0.06;_eAED=0.06;_QI=275|1|1|1|1|1|6|437|503
chr3_3 exon 69869489 69870111 69869489 ID=Msa0468260-mRNA-1:exon:16677;Parent=Msa0468260-mRNA-1
chr3_3 exon 69870200 69870469 69870200 ID=Msa0468260-mRNA-1:exon:16676;Parent=Msa0468260-mRNA-1
chr3_3 exon 69871198 69871473 69871198 ID=Msa0468260-mRNA-1:exon:16675;Parent=Msa0468260-mRNA-1
chr3_3 exon 69871598 69871717 69871598 ID=Msa0468260-mRNA-1:exon:16674;Parent=Msa0468260-mRNA-1
chr3_3 exon 69872410 69872475 69872410 ID=Msa0468260-mRNA-1:exon:16673;Parent=Msa0468260-mRNA-1
chr3_3 exon 69872696 69873564 69872696 ID=Msa0468260-mRNA-1:exon:16672;Parent=Msa0468260-mRNA-1
chr3_3 five_prime_UTR 69873290 69873564 69873290 ID=Msa0468260-mRNA-1:five_prime_utr;Parent=Msa0468260-mRNA-1
chr3_3 CDS 69872696 69873289 69872696 ID=Msa0468260-mRNA-1:cds;Parent=Msa0468260-mRNA-1
chr3_3 CDS 69872410 69872475 69872410 ID=Msa0468260-mRNA-1:cds;Parent=Msa0468260-mRNA-1
chr3_3 CDS 69871598 69871717 69871598 ID=Msa0468260-mRNA-1:cds;Parent=Msa0468260-mRNA-1
chr3_3 CDS 69871198 69871473 69871198 ID=Msa0468260-mRNA-1:cds;Parent=Msa0468260-mRNA-1
chr3_3 CDS 69870200 69870469 69870200 ID=Msa0468260-mRNA-1:cds;Parent=Msa0468260-mRNA-1
chr3_3 CDS 69869926 69870111 69869926 ID=Msa0468260-mRNA-1:cds;Parent=Msa0468260-mRNA-1
chr3_3 three_prime_UTR 69869489 69869925 69869489 ID=Msa0468260-mRNA-1:three_prime_utr;Parent=Msa0468260-mRNA-1
Gene Sequence

>Msa0468260

ATGGAACCGTCTCAACAATCTCAATCACATTTGGCTTCTTCTCATCATGTTCAACATATTTCCCCTGTTTCCAATCATCATCCTGAACCCTCATATCTCAATCAATTGACCCAACTTGATGATGAGCTTCAGAACAAGTTGGATTTGAAAGATGAGGATGGAATTGAGGAAAAAGGTTCAAACTTTGAAGTTGGTGATGAAGCTGCTGTTCTAGAATTTGATAAGGGACAAGAGTGTCATGACGATGATGAGAATGTGAATGAGAAGGTGAGTACTGATGTTGATGAAGTGAGTGGAATTGATGATAAAGGTTCAAACTTTGAAGATGATGATGAAAAATCTGCCACAGTATGTGAGAAGGGACAAGGGTTTCATCAAGTTCATGATGATGATGGTTGGAGTGGTGATTATGTGGTGAGTGATTGGATTGAGAATGAGGTGGTGAGTGGATATGTTGATGAAAGAAGCAATGGTGGAGCTGAACAGTATCCATTGAGGCCTGAAGCTGAAGACTGTTCTTTTTATCTTAAGACTGGAACTTGCAAATTTGGATTCAATTGCAAGTTTAATCACCCTGTTTGGAGAAGAAACCAGGTTTTTAGAGAGAGGGCAGGAGAAAGAGATGAATTGGAGGAAAGATCAAGCCAGATAGAATGCAAGTATTATTCAAGATCTGGGGGTTGTAAGTTTGGAAAGGATTGTAAATTTGATCACACCAGAGGAAAGTTTTCAGCAGATCAAGTTTTACAGCTTAACTTCCTTGGCCTACCTATTCGTTTGGGTGAGAAAGAATGCCCTTATTACATGCGCACCGGTTCTTGTAAGTTTGGAGCAAACTGCAAGTTTAATCATCCCGATCCTACATCTGTTGGAGGGTATGACTCCACTTTAGGATATGGTAATGGAAGTTCTATTTCATTCCAAGACGTGTCACAATCATCTACACCCCCATGGTCTTCTACCAGAAAATTCAATGAAACTGCTCCTTTTGCGCCAATCATAATTTCACCGACTCCAGGGGCTTCTCCCCGAAGTTCTGACTGGAATGGATATCAGGCACCTTTCTATTTTTCTGAGAGGAGTATGCATCCACCTTCACCATATGCCGTGAACAATCCAGCTATGGAAACGAATGCTTATATACACCGTCATAAGCATACACCAGTTGAAGAGTTTCCAGAAAGACCTGGTGAACCTGAATGCAGTTTCTTCTTAAAAACCGGGGATTGCAAATTTAAATCCCATTGCAAATTTCATCATCCAAAGAACCGGATTACAAAATTACCTCCATGCAACCTAAGTGACAAGGGTCTGCCTTTGAGACCTGGCCAGAATGTGTGCACTCATTACAACCGATATGGAATTTGCAAATTTGGACCAGCTTGTAAGTATGACCACCCGATAAACCTACCGCCCCCAACTATGCCTGGACGTTATCAACAATCCTCTCACACAAACTCGGCTAGTATTGAGGAGGCTGGAAGTGGAGGAGCTAGTGATGCAACAATTTAA
Protein sequence

>Msa0468260

MEPSQQSQSHLASSHHVQHISPVSNHHPEPSYLNQLTQLDDELQNKLDLKDEDGIEEKGSNFEVGDEAAVLEFDKGQECHDDDENVNEKVSTDVDEVSGIDDKGSNFEDDDEKSATVCEKGQGFHQVHDDDGWSGDYVVSDWIENEVVSGYVDERSNGGAEQYPLRPEAEDCSFYLKTGTCKFGFNCKFNHPVWRRNQVFRERAGERDELEERSSQIECKYYSRSGGCKFGKDCKFDHTRGKFSADQVLQLNFLGLPIRLGEKECPYYMRTGSCKFGANCKFNHPDPTSVGGYDSTLGYGNGSSISFQDVSQSSTPPWSSTRKFNETAPFAPIIISPTPGASPRSSDWNGYQAPFYFSERSMHPPSPYAVNNPAMETNAYIHRHKHTPVEEFPERPGEPECSFFLKTGDCKFKSHCKFHHPKNRITKLPPCNLSDKGLPLRPGQNVCTHYNRYGICKFGPACKYDHPINLPPPTMPGRYQQSSHTNSASIEEAGSGGASDATI*