AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0471250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0471250 MtrunA17_Chr3g0125991 93.443 366 24 0 1 366 1 366 0.0 720
Msa0471250 MtrunA17_Chr3g0126011 92.350 366 28 0 1 366 1 366 0.0 717
Msa0471250 MtrunA17_Chr3g0125781 79.781 366 70 1 5 366 4 369 0.0 614
Msa0471250 MtrunA17_Chr3g0125981 76.294 367 82 1 3 364 2 368 0.0 590
Msa0471250 MtrunA17_Chr1g0159321 74.709 344 84 1 26 366 47 390 0.0 551
Msa0471250 MtrunA17_Chr4g0045661 65.143 350 114 3 23 366 46 393 2.66e-171 483
Msa0471250 MtrunA17_Chr5g0406961 65.143 350 114 3 23 366 97 444 6.53e-171 483
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0471250 AT1G43800.1 69.209 354 104 2 18 366 38 391 0.0 523
Msa0471250 AT3G02630.1 65.680 338 112 2 29 364 59 394 2.14e-167 473
Msa0471250 AT5G16240.1 63.610 349 118 3 23 364 46 392 4.20e-166 470
Msa0471250 AT2G43710.2 64.307 339 118 2 29 366 65 401 9.55e-164 464
Msa0471250 AT2G43710.1 64.307 339 118 2 29 366 65 401 1.08e-163 464
Msa0471250 AT3G02610.1 59.946 372 129 5 14 366 41 411 1.31e-163 464
Msa0471250 AT3G02610.2 59.946 372 129 5 14 366 43 413 1.31e-163 464
Msa0471250 AT3G02620.1 60.165 364 133 4 14 366 41 403 1.38e-162 461
Msa0471250 AT3G02620.2 60.165 364 133 4 14 366 44 406 1.58e-162 461
Msa0471250 AT5G16230.1 61.988 342 125 3 29 366 61 401 4.11e-161 457
Msa0471250 AT5G16230.2 62.270 326 118 3 45 366 1 325 5.39e-155 439
Msa0471250 AT3G02620.3 62.195 328 117 3 45 366 1 327 1.98e-153 435

Find 93 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATGATTATCTTGTGGTTT+TGG 0.271084 3_3:-73455517 Msa0471250:CDS
AATCTTGTGCTGCATCATTT+TGG 0.271325 3_3:+73454487 None:intergenic
GAGACATTGAGTGTGTCTTT+TGG 0.299881 3_3:+73455718 None:intergenic
CCAACTTGTTTATCCATGTT+TGG 0.336928 3_3:+73455457 None:intergenic
GTCTCACACCATGGGCTATA+TGG 0.350432 3_3:-73455407 Msa0471250:CDS
GTGGACTTGCACCTAGATTA+AGG 0.352719 3_3:-73454271 Msa0471250:CDS
CTCTCAGGGCCTGCACTTGA+TGG 0.353548 3_3:+73455559 None:intergenic
TTGTTCATGGTGTAGGATTT+GGG 0.366166 3_3:-73454737 Msa0471250:intron
GGGCTATATGGTCACGCTCT+TGG 0.370720 3_3:-73455395 Msa0471250:CDS
TGCCAGCTCCAATCAAATAT+TGG 0.374430 3_3:+73455283 None:intergenic
TGTTCAAATCACTAGAGAAA+TGG 0.378630 3_3:-73455680 Msa0471250:CDS
CTTGATTAGACGATGGAGAT+TGG 0.392791 3_3:-73454345 Msa0471250:CDS
GTTCAAATCACTAGAGAAAT+GGG 0.399384 3_3:-73455679 Msa0471250:CDS
AAGACTATCCAATATTTGAT+TGG 0.400329 3_3:-73455291 Msa0471250:CDS
GCTCAGCCGTTCGATCTCTC+AGG 0.408301 3_3:+73455544 None:intergenic
TCTGCGGTGGCACAACGTAT+TGG 0.420320 3_3:-73454407 Msa0471250:CDS
GGCTGCCAGCATTCCTCCAC+AGG 0.422913 3_3:+73455627 None:intergenic
GTGTACACAAAACCCATGTA+TGG 0.438769 3_3:+73454701 None:intergenic
TCTTCTAGTTTCCTTAATCT+AGG 0.439715 3_3:+73454260 None:intergenic
GGGCCTTTAGATAACATTGT+AGG 0.442941 3_3:+73455763 None:intergenic
GATGGAGTTGGCGACGAGAC+TGG 0.447459 3_3:-73455435 Msa0471250:CDS
AGATCAACAACAAATTTACT+TGG 0.459272 3_3:-73454214 Msa0471250:CDS
ATCCAATATTTGATTGGAGC+TGG 0.463614 3_3:-73455285 Msa0471250:CDS
GGCCTTTAGATAACATTGTA+GGG 0.464982 3_3:+73455764 None:intergenic
TACCGAAAACAACCCATACA+TGG 0.467155 3_3:-73454714 Msa0471250:CDS
CTCGTACTTGCACGCGTATG+CGG 0.473722 3_3:-73454608 Msa0471250:CDS
AGTGGATCCCACCGAGGCTA+TGG 0.482931 3_3:-73454519 Msa0471250:CDS
GGCTATGGCCACCATAGCCT+CGG 0.489653 3_3:+73454508 None:intergenic
TGGAGATTGGAGAAGCTTGA+GGG 0.490817 3_3:-73454332 Msa0471250:CDS
TCTCTTTAGCCAACCTACCT+AGG 0.494048 3_3:+73454639 None:intergenic
TTCACAAAATGTGTTGCCAT+TGG 0.497194 3_3:-73455655 Msa0471250:CDS
AAAACCACAAGATAATCATC+TGG 0.498146 3_3:+73455519 None:intergenic
TTTATCCATGTTTGGTACGT+AGG 0.501588 3_3:+73455465 None:intergenic
TCGTACTTGCACGCGTATGC+GGG 0.518633 3_3:-73454607 Msa0471250:CDS
TCGGAAGGTAGTGATGAGTC+TGG 0.523237 3_3:+73455591 None:intergenic
TCCCCTACAATGTTATCTAA+AGG 0.527355 3_3:-73455766 Msa0471250:CDS
GCTTCCAGATGATTATCTTG+TGG 0.527931 3_3:-73455523 Msa0471250:CDS
CCCGCTCACTTGATGCATGA+TGG 0.529067 3_3:-73454458 Msa0471250:CDS
CCAAACATGGATAAACAAGT+TGG 0.530351 3_3:-73455457 Msa0471250:CDS
CATTGCCTACGTACCAAACA+TGG 0.538689 3_3:-73455470 Msa0471250:CDS
ATATTTGATTGGAGCTGGCA+TGG 0.538813 3_3:-73455280 Msa0471250:intron
TTAAACCTGTGGAGGAATGC+TGG 0.548548 3_3:-73455632 Msa0471250:CDS
CCATCATGCATCAAGTGAGC+GGG 0.549039 3_3:+73454458 None:intergenic
AAAACTTATCTCTATCTCTC+CGG 0.550395 3_3:-73455339 Msa0471250:CDS
AAATGTGGCTCGCTCTTGAA+AGG 0.555847 3_3:+73454676 None:intergenic
ATTGTAGGGGAGTCCACGCA+TGG 0.558263 3_3:+73455778 None:intergenic
ATAAACAAGTTGGATGGAGT+TGG 0.559959 3_3:-73455447 Msa0471250:CDS
GGATCCCACCGAGGCTATGG+TGG 0.562827 3_3:-73454516 Msa0471250:CDS
ATGGAGATTGGAGAAGCTTG+AGG 0.564413 3_3:-73454333 Msa0471250:CDS
CATGCACATATCAACTCTAC+CGG 0.564833 3_3:+73455320 None:intergenic
TGTGGAAAAGCTTCTAGAAG+TGG 0.566245 3_3:-73454537 Msa0471250:CDS
TGAGTTCATCCATCAAGTGC+AGG 0.567672 3_3:-73455568 Msa0471250:CDS
TCCTCCACAGGTTTAACCAA+TGG 0.568150 3_3:+73455639 None:intergenic
TATGGCCACCATAGCCTCGG+TGG 0.570103 3_3:+73454511 None:intergenic
ACATGGATAAACAAGTTGGA+TGG 0.572710 3_3:-73455453 Msa0471250:CDS
CTCAGCCGTTCGATCTCTCA+GGG 0.579284 3_3:+73455545 None:intergenic
AAAGTAAATTAAACATTGAG+AGG 0.580090 3_3:+73454151 None:intergenic
CTTGAGGGTTTGACAAGCGA+AGG 0.581134 3_3:-73454317 Msa0471250:CDS
CGCATACGCGTGCAAGTACG+AGG 0.584480 3_3:+73454609 None:intergenic
ACACGGAAACCTAGGTAGGT+TGG 0.591464 3_3:-73454648 Msa0471250:CDS
CAACATTATGCACCCATGCG+TGG 0.592760 3_3:-73455791 Msa0471250:CDS
TTTGTATCACACGGAAACCT+AGG 0.594979 3_3:-73454656 Msa0471250:CDS
TGGTTCAAGTCTCACACCAT+GGG 0.600203 3_3:-73455415 Msa0471250:CDS
TAGAGTTCTTGATTAGACGA+TGG 0.605110 3_3:-73454352 Msa0471250:CDS
TCCATCATGCATCAAGTGAG+CGG 0.608659 3_3:+73454457 None:intergenic
CTGGTTCAAGTCTCACACCA+TGG 0.612232 3_3:-73455416 Msa0471250:CDS
GCAGGCCCTGAGAGATCGAA+CGG 0.616767 3_3:-73455550 Msa0471250:CDS
GTAAATTAAACATTGAGAGG+CGG 0.618969 3_3:+73454154 None:intergenic
ATGGCCACCATAGCCTCGGT+GGG 0.619166 3_3:+73454512 None:intergenic
GCCTTTAGATAACATTGTAG+GGG 0.623075 3_3:+73455765 None:intergenic
CGAGCCACATTTGTATCACA+CGG 0.623581 3_3:-73454665 Msa0471250:CDS
GAGCGTGACCATATAGCCCA+TGG 0.625365 3_3:+73455399 None:intergenic
ACCGAAAACAACCCATACAT+GGG 0.628237 3_3:-73454713 Msa0471250:CDS
TATCAAATTAACAATGACGA+TGG 0.630822 3_3:-73455814 Msa0471250:exon
GCCATTGGTTAAACCTGTGG+AGG 0.632502 3_3:-73455640 Msa0471250:CDS
GGTAGGTTGGCTAAAGAGAG+AGG 0.635441 3_3:-73454635 Msa0471250:CDS
TCCTCACATATGCATTCTCG+TGG 0.635762 3_3:+73454561 None:intergenic
TGTACACAAAACCCATGTAT+GGG 0.636404 3_3:+73454702 None:intergenic
TATCACACGGAAACCTAGGT+AGG 0.636743 3_3:-73454652 Msa0471250:CDS
CTCGTGGCGCTTCTCATCAG+CGG 0.641370 3_3:+73454577 None:intergenic
GAATGCATATGTGAGGATTG+TGG 0.642224 3_3:-73454555 Msa0471250:CDS
TTGTAGGGGAGTCCACGCAT+GGG 0.642906 3_3:+73455779 None:intergenic
GTTGCCATTGGTTAAACCTG+TGG 0.644205 3_3:-73455643 Msa0471250:CDS
CATGCACAAGAGTTCGTGTG+TGG 0.649922 3_3:-73454290 Msa0471250:CDS
GAGTCTGGCAACAAATTATG+TGG 0.650290 3_3:+73455606 None:intergenic
CACTTGATGGATGAACTCAT+CGG 0.663477 3_3:+73455572 None:intergenic
ACCGCTGAAGAAAATAGACA+TGG 0.664992 3_3:-73455372 Msa0471250:CDS
GCCACGAGAATGCATATGTG+AGG 0.667191 3_3:-73454562 Msa0471250:CDS
TGATGGATGAACTCATCGGA+AGG 0.673548 3_3:+73455576 None:intergenic
GGCGGCTATAAAATTTCACA+AGG 0.686917 3_3:+73454172 None:intergenic
GTTTCCGTGTGATACAAATG+TGG 0.700218 3_3:+73454661 None:intergenic
TCTAGAAGTGGATCCCACCG+AGG 0.721401 3_3:-73454525 Msa0471250:CDS
GGTTGGTAGCATGATCACTG+AGG 0.753309 3_3:-73455496 Msa0471250:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTATGAAAAATGTTATT+TGG + chr3_3:73455083-73455102 None:intergenic 10.0%
!! AATTTATGAAAAATGTTATT+TGG + chr3_3:73455083-73455102 None:intergenic 10.0%
!! ATAAATTATTTGATTGTTCA+TGG - chr3_3:73455096-73455115 Msa0471250:intron 15.0%
!! ATAAATTATTTGATTGTTCA+TGG - chr3_3:73455096-73455115 Msa0471250:intron 15.0%
!! TGTAATAAATCATATGTGTA+TGG + chr3_3:73454866-73454885 None:intergenic 20.0%
!! AAAGTAAATTAAACATTGAG+AGG + chr3_3:73455698-73455717 None:intergenic 20.0%
!! TGTAATAAATCATATGTGTA+TGG + chr3_3:73454866-73454885 None:intergenic 20.0%
!! AAAGTAAATTAAACATTGAG+AGG + chr3_3:73455698-73455717 None:intergenic 20.0%
! TATCAAATTAACAATGACGA+TGG - chr3_3:73454032-73454051 Msa0471250:three_prime_UTR 25.0%
! AAGACTATCCAATATTTGAT+TGG - chr3_3:73454555-73454574 Msa0471250:CDS 25.0%
! AAACTAACGAGAATAACAAA+TGG - chr3_3:73454765-73454784 Msa0471250:intron 25.0%
!! TGTCATTTGTAATACTATGT+GGG + chr3_3:73454835-73454854 None:intergenic 25.0%
!! ATGTCATTTGTAATACTATG+TGG + chr3_3:73454836-73454855 None:intergenic 25.0%
! ATGTGATAGATAGAATATGT+TGG + chr3_3:73455019-73455038 None:intergenic 25.0%
! AGATCAACAACAAATTTACT+TGG - chr3_3:73455632-73455651 Msa0471250:CDS 25.0%
!! TGATTTTCTTGTCTTTATAC+AGG - chr3_3:73455718-73455737 Msa0471250:CDS 25.0%
! TATCAAATTAACAATGACGA+TGG - chr3_3:73454032-73454051 Msa0471250:three_prime_UTR 25.0%
! AAGACTATCCAATATTTGAT+TGG - chr3_3:73454555-73454574 Msa0471250:CDS 25.0%
! AAACTAACGAGAATAACAAA+TGG - chr3_3:73454765-73454784 Msa0471250:intron 25.0%
!! TGTCATTTGTAATACTATGT+GGG + chr3_3:73454835-73454854 None:intergenic 25.0%
!! ATGTCATTTGTAATACTATG+TGG + chr3_3:73454836-73454855 None:intergenic 25.0%
! ATGTGATAGATAGAATATGT+TGG + chr3_3:73455019-73455038 None:intergenic 25.0%
! AGATCAACAACAAATTTACT+TGG - chr3_3:73455632-73455651 Msa0471250:CDS 25.0%
!! TGATTTTCTTGTCTTTATAC+AGG - chr3_3:73455718-73455737 Msa0471250:CDS 25.0%
!! TTTTAGGAATGTTTTGGATG+GGG + chr3_3:73454106-73454125 None:intergenic 30.0%
!!! GTTTTAGGAATGTTTTGGAT+GGG + chr3_3:73454107-73454126 None:intergenic 30.0%
! TTGAACACTTCTTTCTTTTC+AGG + chr3_3:73454153-73454172 None:intergenic 30.0%
TGTTCAAATCACTAGAGAAA+TGG - chr3_3:73454166-73454185 Msa0471250:three_prime_UTR 30.0%
GTTCAAATCACTAGAGAAAT+GGG - chr3_3:73454167-73454186 Msa0471250:three_prime_UTR 30.0%
AAAACCACAAGATAATCATC+TGG + chr3_3:73454330-73454349 None:intergenic 30.0%
! AGATGATTATCTTGTGGTTT+TGG - chr3_3:73454329-73454348 Msa0471250:CDS 30.0%
AAAACTTATCTCTATCTCTC+CGG - chr3_3:73454507-73454526 Msa0471250:CDS 30.0%
! TAAAAGACACCTTCATGTTT+TGG - chr3_3:73454642-73454661 Msa0471250:CDS 30.0%
TTAGTTTCAGGCAAATGTAT+TGG + chr3_3:73454752-73454771 None:intergenic 30.0%
! TTGTTATTCTCGTTAGTTTC+AGG + chr3_3:73454764-73454783 None:intergenic 30.0%
AACGACTGTTAAAAAAACAG+AGG + chr3_3:73454956-73454975 None:intergenic 30.0%
! TTCTATTTCTTTCTCCTTCA+AGG - chr3_3:73455044-73455063 Msa0471250:intron 30.0%
TATTTGATTGTTCATGGTGT+AGG - chr3_3:73455102-73455121 Msa0471250:intron 30.0%
!! TCTTCTAGTTTCCTTAATCT+AGG + chr3_3:73455589-73455608 None:intergenic 30.0%
GTAAATTAAACATTGAGAGG+CGG + chr3_3:73455695-73455714 None:intergenic 30.0%
!! TTTTAGGAATGTTTTGGATG+GGG + chr3_3:73454106-73454125 None:intergenic 30.0%
!!! GTTTTAGGAATGTTTTGGAT+GGG + chr3_3:73454107-73454126 None:intergenic 30.0%
! TTGAACACTTCTTTCTTTTC+AGG + chr3_3:73454153-73454172 None:intergenic 30.0%
TGTTCAAATCACTAGAGAAA+TGG - chr3_3:73454166-73454185 Msa0471250:three_prime_UTR 30.0%
GTTCAAATCACTAGAGAAAT+GGG - chr3_3:73454167-73454186 Msa0471250:three_prime_UTR 30.0%
AAAACCACAAGATAATCATC+TGG + chr3_3:73454330-73454349 None:intergenic 30.0%
! AGATGATTATCTTGTGGTTT+TGG - chr3_3:73454329-73454348 Msa0471250:CDS 30.0%
AAAACTTATCTCTATCTCTC+CGG - chr3_3:73454507-73454526 Msa0471250:CDS 30.0%
! TAAAAGACACCTTCATGTTT+TGG - chr3_3:73454642-73454661 Msa0471250:CDS 30.0%
TTAGTTTCAGGCAAATGTAT+TGG + chr3_3:73454752-73454771 None:intergenic 30.0%
! TTGTTATTCTCGTTAGTTTC+AGG + chr3_3:73454764-73454783 None:intergenic 30.0%
AACGACTGTTAAAAAAACAG+AGG + chr3_3:73454956-73454975 None:intergenic 30.0%
! TTCTATTTCTTTCTCCTTCA+AGG - chr3_3:73455044-73455063 Msa0471250:intron 30.0%
TATTTGATTGTTCATGGTGT+AGG - chr3_3:73455102-73455121 Msa0471250:intron 30.0%
!! TCTTCTAGTTTCCTTAATCT+AGG + chr3_3:73455589-73455608 None:intergenic 30.0%
GTAAATTAAACATTGAGAGG+CGG + chr3_3:73455695-73455714 None:intergenic 30.0%
TCCCCTACAATGTTATCTAA+AGG - chr3_3:73454080-73454099 Msa0471250:three_prime_UTR 35.0%
GCCTTTAGATAACATTGTAG+GGG + chr3_3:73454084-73454103 None:intergenic 35.0%
GGCCTTTAGATAACATTGTA+GGG + chr3_3:73454085-73454104 None:intergenic 35.0%
!!! GGTTTTAGGAATGTTTTGGA+TGG + chr3_3:73454108-73454127 None:intergenic 35.0%
!!! TTGGGGTTTTAGGAATGTTT+TGG + chr3_3:73454112-73454131 None:intergenic 35.0%
! AGACATTGAGTGTGTCTTTT+GGG + chr3_3:73454130-73454149 None:intergenic 35.0%
TTCACAAAATGTGTTGCCAT+TGG - chr3_3:73454191-73454210 Msa0471250:CDS 35.0%
!!! GATTATCTTGTGGTTTTGGT+TGG - chr3_3:73454333-73454352 Msa0471250:CDS 35.0%
TTTATCCATGTTTGGTACGT+AGG + chr3_3:73454384-73454403 None:intergenic 35.0%
! CCAACTTGTTTATCCATGTT+TGG + chr3_3:73454392-73454411 None:intergenic 35.0%
CCAAACATGGATAAACAAGT+TGG - chr3_3:73454389-73454408 Msa0471250:CDS 35.0%
! ACATGGATAAACAAGTTGGA+TGG - chr3_3:73454393-73454412 Msa0471250:CDS 35.0%
! ATAAACAAGTTGGATGGAGT+TGG - chr3_3:73454399-73454418 Msa0471250:CDS 35.0%
! TCCATGTCTATTTTCTTCAG+CGG + chr3_3:73454478-73454497 None:intergenic 35.0%
ATCCAATATTTGATTGGAGC+TGG - chr3_3:73454561-73454580 Msa0471250:CDS 35.0%
ATAAACGAGCCAAAACATGA+AGG + chr3_3:73454654-73454673 None:intergenic 35.0%
GACATATGCATAAACCTTGA+AGG + chr3_3:73455061-73455080 None:intergenic 35.0%
ATTGTTCATGGTGTAGGATT+TGG - chr3_3:73455108-73455127 Msa0471250:intron 35.0%
TTGTTCATGGTGTAGGATTT+GGG - chr3_3:73455109-73455128 Msa0471250:intron 35.0%
TGTACACAAAACCCATGTAT+GGG + chr3_3:73455147-73455166 None:intergenic 35.0%
! AATCTTGTGCTGCATCATTT+TGG + chr3_3:73455362-73455381 None:intergenic 35.0%
! AAAAATGATCGAAGAAGCGT+GGG + chr3_3:73455420-73455439 None:intergenic 35.0%
!! CTTCTTCGATCATTTTTCTG+CGG - chr3_3:73455423-73455442 Msa0471250:CDS 35.0%
TAGAGTTCTTGATTAGACGA+TGG - chr3_3:73455494-73455513 Msa0471250:CDS 35.0%
TCCCCTACAATGTTATCTAA+AGG - chr3_3:73454080-73454099 Msa0471250:three_prime_UTR 35.0%
GCCTTTAGATAACATTGTAG+GGG + chr3_3:73454084-73454103 None:intergenic 35.0%
GGCCTTTAGATAACATTGTA+GGG + chr3_3:73454085-73454104 None:intergenic 35.0%
!!! GGTTTTAGGAATGTTTTGGA+TGG + chr3_3:73454108-73454127 None:intergenic 35.0%
!!! TTGGGGTTTTAGGAATGTTT+TGG + chr3_3:73454112-73454131 None:intergenic 35.0%
! AGACATTGAGTGTGTCTTTT+GGG + chr3_3:73454130-73454149 None:intergenic 35.0%
TTCACAAAATGTGTTGCCAT+TGG - chr3_3:73454191-73454210 Msa0471250:CDS 35.0%
!!! GATTATCTTGTGGTTTTGGT+TGG - chr3_3:73454333-73454352 Msa0471250:CDS 35.0%
TTTATCCATGTTTGGTACGT+AGG + chr3_3:73454384-73454403 None:intergenic 35.0%
! CCAACTTGTTTATCCATGTT+TGG + chr3_3:73454392-73454411 None:intergenic 35.0%
CCAAACATGGATAAACAAGT+TGG - chr3_3:73454389-73454408 Msa0471250:CDS 35.0%
! ACATGGATAAACAAGTTGGA+TGG - chr3_3:73454393-73454412 Msa0471250:CDS 35.0%
! ATAAACAAGTTGGATGGAGT+TGG - chr3_3:73454399-73454418 Msa0471250:CDS 35.0%
! TCCATGTCTATTTTCTTCAG+CGG + chr3_3:73454478-73454497 None:intergenic 35.0%
ATCCAATATTTGATTGGAGC+TGG - chr3_3:73454561-73454580 Msa0471250:CDS 35.0%
ATAAACGAGCCAAAACATGA+AGG + chr3_3:73454654-73454673 None:intergenic 35.0%
GACATATGCATAAACCTTGA+AGG + chr3_3:73455061-73455080 None:intergenic 35.0%
ATTGTTCATGGTGTAGGATT+TGG - chr3_3:73455108-73455127 Msa0471250:intron 35.0%
TTGTTCATGGTGTAGGATTT+GGG - chr3_3:73455109-73455128 Msa0471250:intron 35.0%
TGTACACAAAACCCATGTAT+GGG + chr3_3:73455147-73455166 None:intergenic 35.0%
! AATCTTGTGCTGCATCATTT+TGG + chr3_3:73455362-73455381 None:intergenic 35.0%
! AAAAATGATCGAAGAAGCGT+GGG + chr3_3:73455420-73455439 None:intergenic 35.0%
!! CTTCTTCGATCATTTTTCTG+CGG - chr3_3:73455423-73455442 Msa0471250:CDS 35.0%
TAGAGTTCTTGATTAGACGA+TGG - chr3_3:73455494-73455513 Msa0471250:CDS 35.0%
GGGCCTTTAGATAACATTGT+AGG + chr3_3:73454086-73454105 None:intergenic 40.0%
!!! AGTGTGTCTTTTGGGGTTTT+AGG + chr3_3:73454122-73454141 None:intergenic 40.0%
! GACATTGAGTGTGTCTTTTG+GGG + chr3_3:73454129-73454148 None:intergenic 40.0%
! GAGACATTGAGTGTGTCTTT+TGG + chr3_3:73454131-73454150 None:intergenic 40.0%
GAGTCTGGCAACAAATTATG+TGG + chr3_3:73454243-73454262 None:intergenic 40.0%
! CACTTGATGGATGAACTCAT+CGG + chr3_3:73454277-73454296 None:intergenic 40.0%
GCTTCCAGATGATTATCTTG+TGG - chr3_3:73454323-73454342 Msa0471250:CDS 40.0%
ACCGCTGAAGAAAATAGACA+TGG - chr3_3:73454474-73454493 Msa0471250:CDS 40.0%
CATGCACATATCAACTCTAC+CGG + chr3_3:73454529-73454548 None:intergenic 40.0%
TGCCAGCTCCAATCAAATAT+TGG + chr3_3:73454566-73454585 None:intergenic 40.0%
ATATTTGATTGGAGCTGGCA+TGG - chr3_3:73454566-73454585 Msa0471250:CDS 40.0%
TACCGAAAACAACCCATACA+TGG - chr3_3:73455132-73455151 Msa0471250:intron 40.0%
ACCGAAAACAACCCATACAT+GGG - chr3_3:73455133-73455152 Msa0471250:intron 40.0%
!! ACCCATGTATGGGTTGTTTT+CGG + chr3_3:73455137-73455156 None:intergenic 40.0%
GTGTACACAAAACCCATGTA+TGG + chr3_3:73455148-73455167 None:intergenic 40.0%
GTTTCCGTGTGATACAAATG+TGG + chr3_3:73455188-73455207 None:intergenic 40.0%
TTTGTATCACACGGAAACCT+AGG - chr3_3:73455190-73455209 Msa0471250:intron 40.0%
GAATGCATATGTGAGGATTG+TGG - chr3_3:73455291-73455310 Msa0471250:CDS 40.0%
! TGTGGAAAAGCTTCTAGAAG+TGG - chr3_3:73455309-73455328 Msa0471250:CDS 40.0%
GAAAAATGATCGAAGAAGCG+TGG + chr3_3:73455421-73455440 None:intergenic 40.0%
CTTGATTAGACGATGGAGAT+TGG - chr3_3:73455501-73455520 Msa0471250:CDS 40.0%
GGCGGCTATAAAATTTCACA+AGG + chr3_3:73455677-73455696 None:intergenic 40.0%
GGGCCTTTAGATAACATTGT+AGG + chr3_3:73454086-73454105 None:intergenic 40.0%
!!! AGTGTGTCTTTTGGGGTTTT+AGG + chr3_3:73454122-73454141 None:intergenic 40.0%
! GACATTGAGTGTGTCTTTTG+GGG + chr3_3:73454129-73454148 None:intergenic 40.0%
! GAGACATTGAGTGTGTCTTT+TGG + chr3_3:73454131-73454150 None:intergenic 40.0%
GAGTCTGGCAACAAATTATG+TGG + chr3_3:73454243-73454262 None:intergenic 40.0%
! CACTTGATGGATGAACTCAT+CGG + chr3_3:73454277-73454296 None:intergenic 40.0%
GCTTCCAGATGATTATCTTG+TGG - chr3_3:73454323-73454342 Msa0471250:CDS 40.0%
ACCGCTGAAGAAAATAGACA+TGG - chr3_3:73454474-73454493 Msa0471250:CDS 40.0%
CATGCACATATCAACTCTAC+CGG + chr3_3:73454529-73454548 None:intergenic 40.0%
TGCCAGCTCCAATCAAATAT+TGG + chr3_3:73454566-73454585 None:intergenic 40.0%
ATATTTGATTGGAGCTGGCA+TGG - chr3_3:73454566-73454585 Msa0471250:CDS 40.0%
TACCGAAAACAACCCATACA+TGG - chr3_3:73455132-73455151 Msa0471250:intron 40.0%
ACCGAAAACAACCCATACAT+GGG - chr3_3:73455133-73455152 Msa0471250:intron 40.0%
!! ACCCATGTATGGGTTGTTTT+CGG + chr3_3:73455137-73455156 None:intergenic 40.0%
GTGTACACAAAACCCATGTA+TGG + chr3_3:73455148-73455167 None:intergenic 40.0%
GTTTCCGTGTGATACAAATG+TGG + chr3_3:73455188-73455207 None:intergenic 40.0%
TTTGTATCACACGGAAACCT+AGG - chr3_3:73455190-73455209 Msa0471250:intron 40.0%
GAATGCATATGTGAGGATTG+TGG - chr3_3:73455291-73455310 Msa0471250:CDS 40.0%
! TGTGGAAAAGCTTCTAGAAG+TGG - chr3_3:73455309-73455328 Msa0471250:CDS 40.0%
GAAAAATGATCGAAGAAGCG+TGG + chr3_3:73455421-73455440 None:intergenic 40.0%
CTTGATTAGACGATGGAGAT+TGG - chr3_3:73455501-73455520 Msa0471250:CDS 40.0%
GGCGGCTATAAAATTTCACA+AGG + chr3_3:73455677-73455696 None:intergenic 40.0%
! GTTGCCATTGGTTAAACCTG+TGG - chr3_3:73454203-73454222 Msa0471250:CDS 45.0%
TCCTCCACAGGTTTAACCAA+TGG + chr3_3:73454210-73454229 None:intergenic 45.0%
TTAAACCTGTGGAGGAATGC+TGG - chr3_3:73454214-73454233 Msa0471250:CDS 45.0%
! TGATGGATGAACTCATCGGA+AGG + chr3_3:73454273-73454292 None:intergenic 45.0%
TGAGTTCATCCATCAAGTGC+AGG - chr3_3:73454278-73454297 Msa0471250:CDS 45.0%
CATTGCCTACGTACCAAACA+TGG - chr3_3:73454376-73454395 Msa0471250:CDS 45.0%
TGGTTCAAGTCTCACACCAT+GGG - chr3_3:73454431-73454450 Msa0471250:CDS 45.0%
AAATGTGGCTCGCTCTTGAA+AGG + chr3_3:73455173-73455192 None:intergenic 45.0%
CGAGCCACATTTGTATCACA+CGG - chr3_3:73455181-73455200 Msa0471250:intron 45.0%
TATCACACGGAAACCTAGGT+AGG - chr3_3:73455194-73455213 Msa0471250:intron 45.0%
TCTCTTTAGCCAACCTACCT+AGG + chr3_3:73455210-73455229 None:intergenic 45.0%
TCCTCACATATGCATTCTCG+TGG + chr3_3:73455288-73455307 None:intergenic 45.0%
!!! GTGCTGCATCATTTTGGCTA+TGG + chr3_3:73455356-73455375 None:intergenic 45.0%
TCCATCATGCATCAAGTGAG+CGG + chr3_3:73455392-73455411 None:intergenic 45.0%
!! CTTCGATCATTTTTCTGCGG+TGG - chr3_3:73455426-73455445 Msa0471250:CDS 45.0%
ATGGAGATTGGAGAAGCTTG+AGG - chr3_3:73455513-73455532 Msa0471250:CDS 45.0%
TGGAGATTGGAGAAGCTTGA+GGG - chr3_3:73455514-73455533 Msa0471250:CDS 45.0%
GTGGACTTGCACCTAGATTA+AGG - chr3_3:73455575-73455594 Msa0471250:CDS 45.0%
! GTTGCCATTGGTTAAACCTG+TGG - chr3_3:73454203-73454222 Msa0471250:CDS 45.0%
TCCTCCACAGGTTTAACCAA+TGG + chr3_3:73454210-73454229 None:intergenic 45.0%
TTAAACCTGTGGAGGAATGC+TGG - chr3_3:73454214-73454233 Msa0471250:CDS 45.0%
! TGATGGATGAACTCATCGGA+AGG + chr3_3:73454273-73454292 None:intergenic 45.0%
TGAGTTCATCCATCAAGTGC+AGG - chr3_3:73454278-73454297 Msa0471250:CDS 45.0%
CATTGCCTACGTACCAAACA+TGG - chr3_3:73454376-73454395 Msa0471250:CDS 45.0%
TGGTTCAAGTCTCACACCAT+GGG - chr3_3:73454431-73454450 Msa0471250:CDS 45.0%
AAATGTGGCTCGCTCTTGAA+AGG + chr3_3:73455173-73455192 None:intergenic 45.0%
CGAGCCACATTTGTATCACA+CGG - chr3_3:73455181-73455200 Msa0471250:intron 45.0%
TATCACACGGAAACCTAGGT+AGG - chr3_3:73455194-73455213 Msa0471250:intron 45.0%
TCTCTTTAGCCAACCTACCT+AGG + chr3_3:73455210-73455229 None:intergenic 45.0%
TCCTCACATATGCATTCTCG+TGG + chr3_3:73455288-73455307 None:intergenic 45.0%
!!! GTGCTGCATCATTTTGGCTA+TGG + chr3_3:73455356-73455375 None:intergenic 45.0%
TCCATCATGCATCAAGTGAG+CGG + chr3_3:73455392-73455411 None:intergenic 45.0%
!! CTTCGATCATTTTTCTGCGG+TGG - chr3_3:73455426-73455445 Msa0471250:CDS 45.0%
ATGGAGATTGGAGAAGCTTG+AGG - chr3_3:73455513-73455532 Msa0471250:CDS 45.0%
TGGAGATTGGAGAAGCTTGA+GGG - chr3_3:73455514-73455533 Msa0471250:CDS 45.0%
GTGGACTTGCACCTAGATTA+AGG - chr3_3:73455575-73455594 Msa0471250:CDS 45.0%
CAACATTATGCACCCATGCG+TGG - chr3_3:73454055-73454074 Msa0471250:three_prime_UTR 50.0%
! GCCATTGGTTAAACCTGTGG+AGG - chr3_3:73454206-73454225 Msa0471250:CDS 50.0%
TCGGAAGGTAGTGATGAGTC+TGG + chr3_3:73454258-73454277 None:intergenic 50.0%
!! GGTTGGTAGCATGATCACTG+AGG - chr3_3:73454350-73454369 Msa0471250:CDS 50.0%
CTGGTTCAAGTCTCACACCA+TGG - chr3_3:73454430-73454449 Msa0471250:CDS 50.0%
! GTCTCACACCATGGGCTATA+TGG - chr3_3:73454439-73454458 Msa0471250:CDS 50.0%
ACACGGAAACCTAGGTAGGT+TGG - chr3_3:73455198-73455217 Msa0471250:intron 50.0%
! GGTAGGTTGGCTAAAGAGAG+AGG - chr3_3:73455211-73455230 Msa0471250:intron 50.0%
GCCACGAGAATGCATATGTG+AGG - chr3_3:73455284-73455303 Msa0471250:CDS 50.0%
CCATCATGCATCAAGTGAGC+GGG + chr3_3:73455391-73455410 None:intergenic 50.0%
CTTGAGGGTTTGACAAGCGA+AGG - chr3_3:73455529-73455548 Msa0471250:CDS 50.0%
CATGCACAAGAGTTCGTGTG+TGG - chr3_3:73455556-73455575 Msa0471250:CDS 50.0%
CAACATTATGCACCCATGCG+TGG - chr3_3:73454055-73454074 Msa0471250:three_prime_UTR 50.0%
! GCCATTGGTTAAACCTGTGG+AGG - chr3_3:73454206-73454225 Msa0471250:CDS 50.0%
TCGGAAGGTAGTGATGAGTC+TGG + chr3_3:73454258-73454277 None:intergenic 50.0%
!! GGTTGGTAGCATGATCACTG+AGG - chr3_3:73454350-73454369 Msa0471250:CDS 50.0%
CTGGTTCAAGTCTCACACCA+TGG - chr3_3:73454430-73454449 Msa0471250:CDS 50.0%
! GTCTCACACCATGGGCTATA+TGG - chr3_3:73454439-73454458 Msa0471250:CDS 50.0%
ACACGGAAACCTAGGTAGGT+TGG - chr3_3:73455198-73455217 Msa0471250:intron 50.0%
! GGTAGGTTGGCTAAAGAGAG+AGG - chr3_3:73455211-73455230 Msa0471250:intron 50.0%
GCCACGAGAATGCATATGTG+AGG - chr3_3:73455284-73455303 Msa0471250:CDS 50.0%
CCATCATGCATCAAGTGAGC+GGG + chr3_3:73455391-73455410 None:intergenic 50.0%
CTTGAGGGTTTGACAAGCGA+AGG - chr3_3:73455529-73455548 Msa0471250:CDS 50.0%
CATGCACAAGAGTTCGTGTG+TGG - chr3_3:73455556-73455575 Msa0471250:CDS 50.0%
TTGTAGGGGAGTCCACGCAT+GGG + chr3_3:73454070-73454089 None:intergenic 55.0%
ATTGTAGGGGAGTCCACGCA+TGG + chr3_3:73454071-73454090 None:intergenic 55.0%
CTCAGCCGTTCGATCTCTCA+GGG + chr3_3:73454304-73454323 None:intergenic 55.0%
GAGCGTGACCATATAGCCCA+TGG + chr3_3:73454450-73454469 None:intergenic 55.0%
GGGCTATATGGTCACGCTCT+TGG - chr3_3:73454451-73454470 Msa0471250:CDS 55.0%
CTCGTACTTGCACGCGTATG+CGG - chr3_3:73455238-73455257 Msa0471250:intron 55.0%
TCGTACTTGCACGCGTATGC+GGG - chr3_3:73455239-73455258 Msa0471250:intron 55.0%
TCTAGAAGTGGATCCCACCG+AGG - chr3_3:73455321-73455340 Msa0471250:CDS 55.0%
CCCGCTCACTTGATGCATGA+TGG - chr3_3:73455388-73455407 Msa0471250:CDS 55.0%
TCTGCGGTGGCACAACGTAT+TGG - chr3_3:73455439-73455458 Msa0471250:CDS 55.0%
TTGTAGGGGAGTCCACGCAT+GGG + chr3_3:73454070-73454089 None:intergenic 55.0%
ATTGTAGGGGAGTCCACGCA+TGG + chr3_3:73454071-73454090 None:intergenic 55.0%
CTCAGCCGTTCGATCTCTCA+GGG + chr3_3:73454304-73454323 None:intergenic 55.0%
GAGCGTGACCATATAGCCCA+TGG + chr3_3:73454450-73454469 None:intergenic 55.0%
GGGCTATATGGTCACGCTCT+TGG - chr3_3:73454451-73454470 Msa0471250:CDS 55.0%
CTCGTACTTGCACGCGTATG+CGG - chr3_3:73455238-73455257 Msa0471250:intron 55.0%
TCGTACTTGCACGCGTATGC+GGG - chr3_3:73455239-73455258 Msa0471250:intron 55.0%
TCTAGAAGTGGATCCCACCG+AGG - chr3_3:73455321-73455340 Msa0471250:CDS 55.0%
CCCGCTCACTTGATGCATGA+TGG - chr3_3:73455388-73455407 Msa0471250:CDS 55.0%
TCTGCGGTGGCACAACGTAT+TGG - chr3_3:73455439-73455458 Msa0471250:CDS 55.0%
CTCTCAGGGCCTGCACTTGA+TGG + chr3_3:73454290-73454309 None:intergenic 60.0%
GCAGGCCCTGAGAGATCGAA+CGG - chr3_3:73454296-73454315 Msa0471250:CDS 60.0%
GCTCAGCCGTTCGATCTCTC+AGG + chr3_3:73454305-73454324 None:intergenic 60.0%
GATGGAGTTGGCGACGAGAC+TGG - chr3_3:73454411-73454430 Msa0471250:CDS 60.0%
CGCATACGCGTGCAAGTACG+AGG + chr3_3:73455240-73455259 None:intergenic 60.0%
CTCGTGGCGCTTCTCATCAG+CGG + chr3_3:73455272-73455291 None:intergenic 60.0%
AGTGGATCCCACCGAGGCTA+TGG - chr3_3:73455327-73455346 Msa0471250:CDS 60.0%
ATGGCCACCATAGCCTCGGT+GGG + chr3_3:73455337-73455356 None:intergenic 60.0%
TATGGCCACCATAGCCTCGG+TGG + chr3_3:73455338-73455357 None:intergenic 60.0%
GGCTATGGCCACCATAGCCT+CGG + chr3_3:73455341-73455360 None:intergenic 60.0%
CTCTCAGGGCCTGCACTTGA+TGG + chr3_3:73454290-73454309 None:intergenic 60.0%
GCAGGCCCTGAGAGATCGAA+CGG - chr3_3:73454296-73454315 Msa0471250:CDS 60.0%
GCTCAGCCGTTCGATCTCTC+AGG + chr3_3:73454305-73454324 None:intergenic 60.0%
GATGGAGTTGGCGACGAGAC+TGG - chr3_3:73454411-73454430 Msa0471250:CDS 60.0%
CGCATACGCGTGCAAGTACG+AGG + chr3_3:73455240-73455259 None:intergenic 60.0%
CTCGTGGCGCTTCTCATCAG+CGG + chr3_3:73455272-73455291 None:intergenic 60.0%
AGTGGATCCCACCGAGGCTA+TGG - chr3_3:73455327-73455346 Msa0471250:CDS 60.0%
ATGGCCACCATAGCCTCGGT+GGG + chr3_3:73455337-73455356 None:intergenic 60.0%
TATGGCCACCATAGCCTCGG+TGG + chr3_3:73455338-73455357 None:intergenic 60.0%
GGCTATGGCCACCATAGCCT+CGG + chr3_3:73455341-73455360 None:intergenic 60.0%
GGCTGCCAGCATTCCTCCAC+AGG + chr3_3:73454222-73454241 None:intergenic 65.0%
GGATCCCACCGAGGCTATGG+TGG - chr3_3:73455330-73455349 Msa0471250:CDS 65.0%
GGCTGCCAGCATTCCTCCAC+AGG + chr3_3:73454222-73454241 None:intergenic 65.0%
GGATCCCACCGAGGCTATGG+TGG - chr3_3:73455330-73455349 Msa0471250:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 73454027 73455841 73454027 ID=Msa0471250;Name=Msa0471250
chr3_3 mRNA 73454027 73455841 73454027 ID=Msa0471250-mRNA-1;Parent=Msa0471250;Name=Msa0471250-mRNA-1;_AED=0.33;_eAED=0.33;_QI=18|1|1|1|1|1|2|160|366
chr3_3 exon 73454027 73454744 73454027 ID=Msa0471250-mRNA-1:exon:18650;Parent=Msa0471250-mRNA-1
chr3_3 exon 73455281 73455841 73455281 ID=Msa0471250-mRNA-1:exon:18649;Parent=Msa0471250-mRNA-1
chr3_3 five_prime_UTR 73455824 73455841 73455824 ID=Msa0471250-mRNA-1:five_prime_utr;Parent=Msa0471250-mRNA-1
chr3_3 CDS 73455281 73455823 73455281 ID=Msa0471250-mRNA-1:cds;Parent=Msa0471250-mRNA-1
chr3_3 CDS 73454187 73454744 73454187 ID=Msa0471250-mRNA-1:cds;Parent=Msa0471250-mRNA-1
chr3_3 three_prime_UTR 73454027 73454186 73454027 ID=Msa0471250-mRNA-1:three_prime_utr;Parent=Msa0471250-mRNA-1
Gene Sequence

>Msa0471250

ATGACGATGGCAACATTATGCACCCATGCGTGGACTCCCCTACAATGTTATCTAAAGGCCCCATCCAAAACATTCCTAAAACCCCAAAAGACACACTCAATGTCTCCTGAAAAGAAAGAAGTGTTCAAATCACTAGAGAAATGGGTTTCACAAAATGTGTTGCCATTGGTTAAACCTGTGGAGGAATGCTGGCAGCCACATAATTTGTTGCCAGACTCATCACTACCTTCCGATGAGTTCATCCATCAAGTGCAGGCCCTGAGAGATCGAACGGCTGAGCTTCCAGATGATTATCTTGTGGTTTTGGTTGGTAGCATGATCACTGAGGAAGCATTGCCTACGTACCAAACATGGATAAACAAGTTGGATGGAGTTGGCGACGAGACTGGTTCAAGTCTCACACCATGGGCTATATGGTCACGCTCTTGGACCGCTGAAGAAAATAGACATGGAGATTTACTCAAAACTTATCTCTATCTCTCCGGTAGAGTTGATATGTGCATGATTGAGAAGACTATCCAATATTTGATTGGAGCTGGCATGGATTTGGGTACCGAAAACAACCCATACATGGGTTTTGTGTACACATCCTTTCAAGAGCGAGCCACATTTGTATCACACGGAAACCTAGGTAGGTTGGCTAAAGAGAGAGGTGACCTCGTACTTGCACGCGTATGCGGGACAATTGCCGCTGATGAGAAGCGCCACGAGAATGCATATGTGAGGATTGTGGAAAAGCTTCTAGAAGTGGATCCCACCGAGGCTATGGTGGCCATAGCCAAAATGATGCAGCACAAGATTTCAATGCCCGCTCACTTGATGCATGATGGACAAGACCCACGCTTCTTCGATCATTTTTCTGCGGTGGCACAACGTATTGGCGTGTACACAACTAAAGATTACATTGATATCTTAGAGTTCTTGATTAGACGATGGAGATTGGAGAAGCTTGAGGGTTTGACAAGCGAAGGACAACATGCACAAGAGTTCGTGTGTGGACTTGCACCTAGATTAAGGAAACTAGAAGAGCGTCTTGATGAGAGAACAAGCAAGATCAACAACAAATTTACTTGGATCTTTAATAAAGAGTTGTCCTTGTGA
Protein sequence

>Msa0471250

MTMATLCTHAWTPLQCYLKAPSKTFLKPQKTHSMSPEKKEVFKSLEKWVSQNVLPLVKPVEECWQPHNLLPDSSLPSDEFIHQVQALRDRTAELPDDYLVVLVGSMITEEALPTYQTWINKLDGVGDETGSSLTPWAIWSRSWTAEENRHGDLLKTYLYLSGRVDMCMIEKTIQYLIGAGMDLGTENNPYMGFVYTSFQERATFVSHGNLGRLAKERGDLVLARVCGTIAADEKRHENAYVRIVEKLLEVDPTEAMVAIAKMMQHKISMPAHLMHDGQDPRFFDHFSAVAQRIGVYTTKDYIDILEFLIRRWRLEKLEGLTSEGQHAQEFVCGLAPRLRKLEERLDERTSKINNKFTWIFNKELSL*