AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0471870

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0471870 MtrunA17_Chr3g0127471 96.117 309 12 0 1 309 1 309 0.0 608
Msa0471870 MtrunA17_Chr1g0158811 64.685 286 90 5 30 309 37 317 7.78e-124 357
Msa0471870 MtrunA17_Chr1g0158821 59.801 301 114 4 11 309 21 316 1.66e-121 351
Msa0471870 MtrunA17_Chr5g0419391 50.000 248 121 3 65 309 12 259 5.82e-82 248
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0471870 AT5G24400.1 72.358 246 65 2 65 309 82 325 2.24e-129 372
Msa0471870 AT3G49360.1 58.964 251 95 3 64 309 4 251 3.28e-103 303
Msa0471870 AT5G24410.1 57.202 243 97 2 71 309 18 257 7.28e-94 279
Msa0471870 AT5G24420.1 54.733 243 104 2 71 309 12 252 2.67e-92 275
Msa0471870 AT1G13700.2 53.200 250 112 3 65 309 12 261 1.71e-88 266
Msa0471870 AT1G13700.3 53.200 250 112 3 65 309 12 261 1.71e-88 266
Msa0471870 AT1G13700.1 53.200 250 112 3 65 309 12 261 1.71e-88 266

Find 108 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTTCACTGTTTGTTTATC+TGG 0.223963 3_3:-74249538 Msa0471870:CDS
TGTTGTGGTAGTGTTTATTT+TGG 0.247482 3_3:-74246886 Msa0471870:three_prime_UTR
ATTCTCTCTGGTGGTGGTTT+TGG 0.248823 3_3:+74247127 None:intergenic
AGATTAACTGGCTTAGCTTT+AGG 0.257023 3_3:+74246912 None:intergenic
AGTAACATGAGATCAAATTT+TGG 0.262107 3_3:+74247244 None:intergenic
GGTGAGTTACTTCACATTAA+TGG 0.265480 3_3:+74246692 None:intergenic
GGGCTAAATGGCATGTGTTT+TGG 0.280558 3_3:-74249352 Msa0471870:CDS
AGAGAGATCAGCGACATATT+TGG 0.303753 3_3:+74249585 None:intergenic
TTTCTAAAATTTCCCTTGAT+AGG 0.310242 3_3:-74246719 Msa0471870:three_prime_UTR
TTACTATCATCATGAGTCTT+TGG 0.318838 3_3:+74249311 None:intergenic
ATTCTATTGAATGGGCTAAA+TGG 0.321159 3_3:-74249364 Msa0471870:CDS
GCTGAAAGTGGACGCAAGTT+TGG 0.321612 3_3:+74249748 None:intergenic
AAGGGAAAGCGATGCGATTC+GGG 0.328256 3_3:+74249777 None:intergenic
AGTGCAATGCGAAGTTCTTT+TGG 0.328325 3_3:-74246749 Msa0471870:three_prime_UTR
AACCATTTCAACTCCCCTTC+AGG 0.336200 3_3:+74246968 None:intergenic
AGGGGAGTTGAAATGGTTCT+TGG 0.344962 3_3:-74246963 Msa0471870:CDS
GTGAACTGCATCCGCTTTAC+CGG 0.345120 3_3:+74247045 None:intergenic
ACCACAACACAAAGATTAAC+TGG 0.348124 3_3:+74246900 None:intergenic
GTGAGTTACTTCACATTAAT+GGG 0.348664 3_3:+74246693 None:intergenic
CTGAAAGTGGACGCAAGTTT+GGG 0.349865 3_3:+74249749 None:intergenic
CACCTGAAGGGGAGTTGAAA+TGG 0.361068 3_3:-74246970 Msa0471870:CDS
AAAGGGAAAGCGATGCGATT+CGG 0.380936 3_3:+74249776 None:intergenic
CATGTAGCTTCTTTGTTCCC+AGG 0.399055 3_3:-74247202 Msa0471870:CDS
TTCTTATAAGTCAAATCAAA+AGG 0.405504 3_3:+74249851 None:intergenic
TGAAAAGCTTCCTGCTGCAT+TGG 0.409834 3_3:-74246996 Msa0471870:CDS
AAAGAAGCTACATGCCCGTC+AGG 0.412823 3_3:+74247211 None:intergenic
AAATTTGATCTCATGTTACT+TGG 0.414181 3_3:-74247241 Msa0471870:CDS
CTTCCACTTTCACCCACTCT+TGG 0.419260 3_3:-74249816 Msa0471870:CDS
AAGGTAAATGTTATTCTCTC+TGG 0.421011 3_3:+74247115 None:intergenic
TGATCTCATGTTACTTGGCA+TGG 0.426304 3_3:-74247236 Msa0471870:CDS
CGTATGTTGATTCTATTGAA+TGG 0.427516 3_3:-74249373 Msa0471870:CDS
GAACCGTTTGTTGTTGTGTT+TGG 0.431600 3_3:+74249684 None:intergenic
AATAAGTTCACTCGCGAAAG+AGG 0.439481 3_3:-74249562 Msa0471870:CDS
GTATGTTGATTCTATTGAAT+GGG 0.446221 3_3:-74249372 Msa0471870:CDS
TGTCAACTATCACTGAGATA+TGG 0.450244 3_3:+74246817 None:intergenic
AATGTTATTCTCTCTGGTGG+TGG 0.456542 3_3:+74247121 None:intergenic
CTGCATTGGTTTCACCTGAA+GGG 0.457863 3_3:-74246982 Msa0471870:CDS
GCTGCATTGGTTTCACCTGA+AGG 0.458392 3_3:-74246983 Msa0471870:CDS
GATCTCATGTTACTTGGCAT+GGG 0.461657 3_3:-74247235 Msa0471870:CDS
GGCAGCTCCCTCAGCTGAAA+GGG 0.463885 3_3:+74247339 None:intergenic
CATTAATGGGAAACCTATCA+AGG 0.468467 3_3:+74246706 None:intergenic
GCTCTTGTGGTGACTGGTAC+CGG 0.472513 3_3:-74247064 Msa0471870:CDS
CATTACTTAGCTGATCAGAT+TGG 0.480743 3_3:-74246606 Msa0471870:three_prime_UTR
TCAGACAACGGTGGAGCAAA+AGG 0.483157 3_3:+74246519 None:intergenic
ATGTAGCTTCTTTGTTCCCA+GGG 0.483220 3_3:-74247201 Msa0471870:CDS
CGGCAGCTCCCTCAGCTGAA+AGG 0.485041 3_3:+74247338 None:intergenic
TTACTTGGCATGGGCCCTGA+CGG 0.487291 3_3:-74247226 Msa0471870:CDS
TAAACATTACCAGGTGGAAT+GGG 0.488424 3_3:+74247376 None:intergenic
CCGTTTGTTGTTGTGTTTGG+CGG 0.492599 3_3:+74249687 None:intergenic
GACGCAAGTTTGGGTTTGAA+AGG 0.493797 3_3:+74249758 None:intergenic
CAACAAACGGTTCGTTTGCA+AGG 0.499477 3_3:-74249674 Msa0471870:CDS
AACGTAACTCAGGCTTCCGC+TGG 0.500834 3_3:+74246629 None:intergenic
TGCATTGGTTTCACCTGAAG+GGG 0.505529 3_3:-74246981 Msa0471870:CDS
TTCACTGTTTGTTTATCTGG+TGG 0.513156 3_3:-74249535 Msa0471870:CDS
GCCAGTTAATCTTTGTGTTG+TGG 0.516060 3_3:-74246901 Msa0471870:three_prime_UTR
TACATCGCTCTTGTGGTGAC+TGG 0.517707 3_3:-74247070 Msa0471870:CDS
TACTTGGCATGGGCCCTGAC+GGG 0.519693 3_3:-74247225 Msa0471870:CDS
TTTCGACCCTTCAGATTCAA+AGG 0.522939 3_3:-74249715 Msa0471870:CDS
CCGCCAAACACAACAACAAA+CGG 0.523427 3_3:-74249687 Msa0471870:CDS
AAAGAGAATTGAGCTGAAAG+TGG 0.523546 3_3:+74249736 None:intergenic
GCAATAATGAGTTTGTGTAA+TGG 0.526403 3_3:-74246864 Msa0471870:three_prime_UTR
TAACCAAGAGTGGGTGAAAG+TGG 0.527010 3_3:+74249813 None:intergenic
TTGAAATGGTTCTTGGACAA+AGG 0.528526 3_3:-74246956 Msa0471870:CDS
CAAGGTGGTGAAATCTTCAA+TGG 0.530806 3_3:-74249656 Msa0471870:CDS
GGTGACACCTTTGAATCTGA+AGG 0.531379 3_3:+74249708 None:intergenic
GAATACGAAAGATGAGGGGT+AGG 0.536006 3_3:+74246670 None:intergenic
ATCTGCTTACATCGCTCTTG+TGG 0.536104 3_3:-74247077 Msa0471870:CDS
TCAGGTGAAACCAATGCAGC+AGG 0.538723 3_3:+74246986 None:intergenic
ATAAACATTACCAGGTGGAA+TGG 0.539576 3_3:+74247375 None:intergenic
CGTGCACAAGAGGGTGCCCT+GGG 0.544738 3_3:+74247185 None:intergenic
GAAATGAATACGAAAGATGA+GGG 0.544826 3_3:+74246665 None:intergenic
TCGTGCACAAGAGGGTGCCC+TGG 0.545437 3_3:+74247184 None:intergenic
CTAAGTAATGAACGTAACTC+AGG 0.548418 3_3:+74246619 None:intergenic
TGGCAGGTGCCCATTCCACC+TGG 0.549190 3_3:-74247385 Msa0471870:intron
ACGCAAGTTTGGGTTTGAAA+GGG 0.549926 3_3:+74249759 None:intergenic
TCTTATAAGTCAAATCAAAA+GGG 0.550216 3_3:+74249852 None:intergenic
CGAGACATGTGTCAAACACT+TGG 0.551519 3_3:-74247308 Msa0471870:CDS
TGATCAGATTGGTATGATGA+AGG 0.559232 3_3:-74246595 Msa0471870:three_prime_UTR
GTGGTCTTTGTAGCAATAAG+AGG 0.559346 3_3:-74246787 Msa0471870:three_prime_UTR
TTGATTGCATAAACATTACC+AGG 0.567415 3_3:+74247367 None:intergenic
AGATGAATTGATCACGGGAA+AGG 0.572185 3_3:+74247096 None:intergenic
AACTAAGCAGTGGACTTAAG+TGG 0.573947 3_3:-74246565 Msa0471870:three_prime_UTR
GTAAATGTTATTCTCTCTGG+TGG 0.576193 3_3:+74247118 None:intergenic
AATACGAAAGATGAGGGGTA+GGG 0.581097 3_3:+74246671 None:intergenic
AATGATGCCCTTTCAGCTGA+GGG 0.585393 3_3:-74247346 Msa0471870:CDS
TGAAATGAATACGAAAGATG+AGG 0.586063 3_3:+74246664 None:intergenic
CAATGATGCCCTTTCAGCTG+AGG 0.587933 3_3:-74247347 Msa0471870:CDS
TAAGCAGATGAATTGATCAC+GGG 0.589684 3_3:+74247091 None:intergenic
GTAAGCAGATGAATTGATCA+CGG 0.608145 3_3:+74247090 None:intergenic
TGTTGAGATGTAACCAAGAG+TGG 0.609804 3_3:+74249803 None:intergenic
TTGTGATTATAGGAAACTGT+TGG 0.611417 3_3:-74249402 Msa0471870:intron
GTGACACCTTTGAATCTGAA+GGG 0.614880 3_3:+74249709 None:intergenic
ATTAATGGGAAACCTATCAA+GGG 0.624653 3_3:+74246707 None:intergenic
CATTCAATAGAATCAACATA+CGG 0.629923 3_3:+74249374 None:intergenic
GACACATGTCTCGTAATCGT+CGG 0.630010 3_3:+74247318 None:intergenic
ATCTCAGTGATAGTTGACAC+TGG 0.636279 3_3:-74246813 Msa0471870:three_prime_UTR
AAGAAGCTACATGCCCGTCA+GGG 0.651830 3_3:+74247212 None:intergenic
CAAACGGTTCGTTTGCAAGG+TGG 0.654959 3_3:-74249671 Msa0471870:CDS
AGGACATAATAACTAAGCAG+TGG 0.661777 3_3:-74246575 Msa0471870:three_prime_UTR
TTCAGTTGAGATGCTTCCAG+CGG 0.669137 3_3:-74246645 Msa0471870:three_prime_UTR
GTTGAGATGTAACCAAGAGT+GGG 0.688248 3_3:+74249804 None:intergenic
AGGGAAAGCGATGCGATTCG+GGG 0.689816 3_3:+74249778 None:intergenic
GGTGACTGGTACCGGTAAAG+CGG 0.693260 3_3:-74247056 Msa0471870:CDS
GCAGTTCACTCTGCACTACG+TGG 0.698853 3_3:-74247031 Msa0471870:CDS
ATAACTTCATCATCAACCAG+TGG 0.699932 3_3:-74247271 Msa0471870:CDS
ATTGCATAAACATTACCAGG+TGG 0.709051 3_3:+74247370 None:intergenic
TGATAGTTGACACTGGACAG+TGG 0.716937 3_3:-74246806 Msa0471870:three_prime_UTR
AAATGAATACGAAAGATGAG+GGG 0.717517 3_3:+74246666 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTGAGTTATTAAATGTT+AGG - chr3_3:74247513-74247532 Msa0471870:intron 15.0%
!!! TCACTTAATTTTTGTTTAAT+AGG + chr3_3:74248074-74248093 None:intergenic 15.0%
!! TAAAAATAACTACAAAATGT+TGG - chr3_3:74248857-74248876 Msa0471870:intron 15.0%
!! TCTTATAAGTCAAATCAAAA+GGG + chr3_3:74246589-74246608 None:intergenic 20.0%
!! TTCTTATAAGTCAAATCAAA+AGG + chr3_3:74246590-74246609 None:intergenic 20.0%
!! AAAAAAAATAGAACATACCT+TGG + chr3_3:74247182-74247201 None:intergenic 20.0%
!!! ATTTTTTATATCACTCAACA+AGG - chr3_3:74247634-74247653 Msa0471870:intron 20.0%
!!! TTTTTTATATCACTCAACAA+GGG - chr3_3:74247635-74247654 Msa0471870:intron 20.0%
!!! AATTAGTGAGATTTTACTTA+TGG - chr3_3:74247832-74247851 Msa0471870:intron 20.0%
!! AAATTTCTTGTACAATACAA+TGG - chr3_3:74248003-74248022 Msa0471870:intron 20.0%
!! TTGATCATTCCTATAAAATA+AGG - chr3_3:74248767-74248786 Msa0471870:intron 20.0%
!!! TTGTAGTTATTTTTACATTG+CGG + chr3_3:74248852-74248871 None:intergenic 20.0%
!!! TTGATTTGTGTTGTGATTAT+AGG - chr3_3:74247026-74247045 Msa0471870:CDS 25.0%
! CATTCAATAGAATCAACATA+CGG + chr3_3:74247067-74247086 None:intergenic 25.0%
!!! GTATGTTGATTCTATTGAAT+GGG - chr3_3:74247066-74247085 Msa0471870:CDS 25.0%
!!! TTTTTTAAGGAGTTGTGTTT+AGG - chr3_3:74247206-74247225 Msa0471870:CDS 25.0%
!!! TTTTTAAGGAGTTGTGTTTA+GGG - chr3_3:74247207-74247226 Msa0471870:CDS 25.0%
!!! TAAACTTGTTTTCCAACTAT+TGG - chr3_3:74247263-74247282 Msa0471870:CDS 25.0%
! TAATTCACTAAATTGAGACA+TGG - chr3_3:74247580-74247599 Msa0471870:intron 25.0%
! TTGACTTTATCTATTCTTGA+TGG + chr3_3:74248123-74248142 None:intergenic 25.0%
!!! GTCAACACTAAAGTATTTTT+AGG - chr3_3:74248138-74248157 Msa0471870:intron 25.0%
! TTATTGCAGACCTAAAAAAA+GGG - chr3_3:74248615-74248634 Msa0471870:intron 25.0%
!!! TTAATAGATGCCCTTTTTTT+AGG + chr3_3:74248628-74248647 None:intergenic 25.0%
! ACTAATAGAACACTATGTAA+GGG + chr3_3:74248984-74249003 None:intergenic 25.0%
! TACTAATAGAACACTATGTA+AGG + chr3_3:74248985-74249004 None:intergenic 25.0%
!! AGTAACATGAGATCAAATTT+TGG + chr3_3:74249197-74249216 None:intergenic 25.0%
! AAATTTGATCTCATGTTACT+TGG - chr3_3:74249197-74249216 Msa0471870:intron 25.0%
!!! TTTCTAAAATTTCCCTTGAT+AGG - chr3_3:74249719-74249738 Msa0471870:CDS 25.0%
TTGTGATTATAGGAAACTGT+TGG - chr3_3:74247036-74247055 Msa0471870:CDS 30.0%
!! CGTATGTTGATTCTATTGAA+TGG - chr3_3:74247065-74247084 Msa0471870:CDS 30.0%
!! ATTCTATTGAATGGGCTAAA+TGG - chr3_3:74247074-74247093 Msa0471870:CDS 30.0%
TTACTATCATCATGAGTCTT+TGG + chr3_3:74247130-74247149 None:intergenic 30.0%
!! AACTATAAGCTTGCTTTTGA+TGG - chr3_3:74247148-74247167 Msa0471870:CDS 30.0%
!! ACTATAAGCTTGCTTTTGAT+GGG - chr3_3:74247149-74247168 Msa0471870:CDS 30.0%
AAGTTCAATCTTCCAATAGT+TGG + chr3_3:74247278-74247297 None:intergenic 30.0%
! CTGAAGTATAAATGCTACTT+AGG - chr3_3:74247445-74247464 Msa0471870:intron 30.0%
AGAAAAAGATGAAGAGTCAT+AGG - chr3_3:74247692-74247711 Msa0471870:intron 30.0%
AAAGTACAGTTTGCATATGT+GGG + chr3_3:74247767-74247786 None:intergenic 30.0%
TAATTGCATTACACTACTAG+TGG + chr3_3:74247817-74247836 None:intergenic 30.0%
TATATTCCTTTGTGTATACC+CGG + chr3_3:74247942-74247961 None:intergenic 30.0%
TTCTTGATGGAACTTAATGT+TGG + chr3_3:74248110-74248129 None:intergenic 30.0%
TTAGGAAGAGTTGAAAAAAG+AGG - chr3_3:74248156-74248175 Msa0471870:intron 30.0%
! TCACAATCAGAAAACCATTT+TGG - chr3_3:74248219-74248238 Msa0471870:intron 30.0%
!!! TCAGAAAACCATTTTGGAAT+TGG - chr3_3:74248225-74248244 Msa0471870:intron 30.0%
!! TAGTACAAAGTGTTGACAAA+CGG + chr3_3:74248424-74248443 None:intergenic 30.0%
CAACACTTTGTACTAAATAG+CGG - chr3_3:74248429-74248448 Msa0471870:intron 30.0%
AGAGAGATTCATATCAAACT+AGG - chr3_3:74248579-74248598 Msa0471870:intron 30.0%
GTTATTGCAGACCTAAAAAA+AGG - chr3_3:74248614-74248633 Msa0471870:intron 30.0%
!! AAAAAAGGGCATCTATTAAG+TGG - chr3_3:74248629-74248648 Msa0471870:intron 30.0%
TTTACATTGCGGTTATAAAG+TGG + chr3_3:74248841-74248860 None:intergenic 30.0%
CTAATAGAACACTATGTAAG+GGG + chr3_3:74248983-74249002 None:intergenic 30.0%
TTGATTGCATAAACATTACC+AGG + chr3_3:74249074-74249093 None:intergenic 30.0%
!! ATCAAATTTTGGAAATCCAC+TGG + chr3_3:74249186-74249205 None:intergenic 30.0%
TTGTGCACGAGAATAAAAAA+TGG - chr3_3:74249267-74249286 Msa0471870:intron 30.0%
TGTGCACGAGAATAAAAAAT+GGG - chr3_3:74249268-74249287 Msa0471870:intron 30.0%
AAGGTAAATGTTATTCTCTC+TGG + chr3_3:74249326-74249345 None:intergenic 30.0%
!! TGTTGTGGTAGTGTTTATTT+TGG - chr3_3:74249552-74249571 Msa0471870:CDS 30.0%
GCAATAATGAGTTTGTGTAA+TGG - chr3_3:74249574-74249593 Msa0471870:CDS 30.0%
ATTAATGGGAAACCTATCAA+GGG + chr3_3:74249734-74249753 None:intergenic 30.0%
GTGAGTTACTTCACATTAAT+GGG + chr3_3:74249748-74249767 None:intergenic 30.0%
AAATGAATACGAAAGATGAG+GGG + chr3_3:74249775-74249794 None:intergenic 30.0%
GAAATGAATACGAAAGATGA+GGG + chr3_3:74249776-74249795 None:intergenic 30.0%
TGAAATGAATACGAAAGATG+AGG + chr3_3:74249777-74249796 None:intergenic 30.0%
!! TAGTGTTACGCTTCACTTTT+TGG - chr3_3:74246537-74246556 Msa0471870:three_prime_UTR 35.0%
!!! TTACGCTTCACTTTTTGGTT+TGG - chr3_3:74246542-74246561 Msa0471870:three_prime_UTR 35.0%
AAAGAGAATTGAGCTGAAAG+TGG + chr3_3:74246705-74246724 None:intergenic 35.0%
GCTTTCACTGTTTGTTTATC+TGG - chr3_3:74246900-74246919 Msa0471870:three_prime_UTR 35.0%
TTCACTGTTTGTTTATCTGG+TGG - chr3_3:74246903-74246922 Msa0471870:three_prime_UTR 35.0%
!!! TTTTGATGGGTTTCTATCCA+AGG - chr3_3:74247162-74247181 Msa0471870:CDS 35.0%
! TGTTTAGGGTTTAGGGTTTA+GGG - chr3_3:74247221-74247240 Msa0471870:CDS 35.0%
AGTGAATGAATGTAACCACA+TGG - chr3_3:74247364-74247383 Msa0471870:CDS 35.0%
AAAACAAGACACAATCCATG+TGG + chr3_3:74247382-74247401 None:intergenic 35.0%
GTTTGATGTCTGACATTTGT+TGG + chr3_3:74247414-74247433 None:intergenic 35.0%
TTCACTAAATTGAGACATGG+TGG - chr3_3:74247583-74247602 Msa0471870:intron 35.0%
AAGTACAGTTTGCATATGTG+GGG + chr3_3:74247766-74247785 None:intergenic 35.0%
! GAAAGTACAGTTTGCATATG+TGG + chr3_3:74247768-74247787 None:intergenic 35.0%
! AGAAATTTTCTCCAGAAAGC+TGG + chr3_3:74247991-74248010 None:intergenic 35.0%
TTGATGAAAGAAGCAAAGAC+AGG + chr3_3:74248043-74248062 None:intergenic 35.0%
TTGGACAGAGCACATTATAT+AGG - chr3_3:74248244-74248263 Msa0471870:intron 35.0%
TTGAGCCAATTTGTCAATCA+CGG - chr3_3:74248333-74248352 Msa0471870:intron 35.0%
ATCTCTCTGAATGTGAGTAA+CGG + chr3_3:74248567-74248586 None:intergenic 35.0%
GAGATTCATATCAAACTAGG+AGG - chr3_3:74248582-74248601 Msa0471870:intron 35.0%
TTGACAAGTACAAGCATTTC+AGG - chr3_3:74248661-74248680 Msa0471870:intron 35.0%
TGACAAGTACAAGCATTTCA+GGG - chr3_3:74248662-74248681 Msa0471870:intron 35.0%
TATCCTACACAGAGATTCAT+TGG + chr3_3:74248715-74248734 None:intergenic 35.0%
AATGAATCTCTGTGTAGGAT+AGG - chr3_3:74248714-74248733 Msa0471870:intron 35.0%
TTCCTATAAAATAAGGCCAG+AGG - chr3_3:74248774-74248793 Msa0471870:intron 35.0%
TCCTATAAAATAAGGCCAGA+GGG - chr3_3:74248775-74248794 Msa0471870:intron 35.0%
! CAGAGGGGAAATGAATTTTA+GGG - chr3_3:74248791-74248810 Msa0471870:intron 35.0%
TAAACATTACCAGGTGGAAT+GGG + chr3_3:74249065-74249084 None:intergenic 35.0%
ATAAACATTACCAGGTGGAA+TGG + chr3_3:74249066-74249085 None:intergenic 35.0%
ATTGCATAAACATTACCAGG+TGG + chr3_3:74249071-74249090 None:intergenic 35.0%
ATAACTTCATCATCAACCAG+TGG - chr3_3:74249167-74249186 Msa0471870:intron 35.0%
! TTTTATTCTCGTGCACAAGA+GGG + chr3_3:74249265-74249284 None:intergenic 35.0%
! TTTTTATTCTCGTGCACAAG+AGG + chr3_3:74249266-74249285 None:intergenic 35.0%
GTAAATGTTATTCTCTCTGG+TGG + chr3_3:74249323-74249342 None:intergenic 35.0%
TAAGCAGATGAATTGATCAC+GGG + chr3_3:74249350-74249369 None:intergenic 35.0%
GTAAGCAGATGAATTGATCA+CGG + chr3_3:74249351-74249370 None:intergenic 35.0%
TTGAAATGGTTCTTGGACAA+AGG - chr3_3:74249482-74249501 Msa0471870:intron 35.0%
! AGATTAACTGGCTTAGCTTT+AGG + chr3_3:74249529-74249548 None:intergenic 35.0%
ACCACAACACAAAGATTAAC+TGG + chr3_3:74249541-74249560 None:intergenic 35.0%
TGTCAACTATCACTGAGATA+TGG + chr3_3:74249624-74249643 None:intergenic 35.0%
CATTAATGGGAAACCTATCA+AGG + chr3_3:74249735-74249754 None:intergenic 35.0%
GGTGAGTTACTTCACATTAA+TGG + chr3_3:74249749-74249768 None:intergenic 35.0%
CTAAGTAATGAACGTAACTC+AGG + chr3_3:74249822-74249841 None:intergenic 35.0%
CATTACTTAGCTGATCAGAT+TGG - chr3_3:74249832-74249851 Msa0471870:exon 35.0%
TGATCAGATTGGTATGATGA+AGG - chr3_3:74249843-74249862 Msa0471870:five_prime_UTR 35.0%
AGGACATAATAACTAAGCAG+TGG - chr3_3:74249863-74249882 Msa0471870:five_prime_UTR 35.0%
GTTGAGATGTAACCAAGAGT+GGG + chr3_3:74246637-74246656 None:intergenic 40.0%
TGTTGAGATGTAACCAAGAG+TGG + chr3_3:74246638-74246657 None:intergenic 40.0%
! ACGCAAGTTTGGGTTTGAAA+GGG + chr3_3:74246682-74246701 None:intergenic 40.0%
TTTCGACCCTTCAGATTCAA+AGG - chr3_3:74246723-74246742 Msa0471870:three_prime_UTR 40.0%
GTGACACCTTTGAATCTGAA+GGG + chr3_3:74246732-74246751 None:intergenic 40.0%
!! GAACCGTTTGTTGTTGTGTT+TGG + chr3_3:74246757-74246776 None:intergenic 40.0%
CAAGGTGGTGAAATCTTCAA+TGG - chr3_3:74246782-74246801 Msa0471870:three_prime_UTR 40.0%
AGAGAGATCAGCGACATATT+TGG + chr3_3:74246856-74246875 None:intergenic 40.0%
AATAAGTTCACTCGCGAAAG+AGG - chr3_3:74246876-74246895 Msa0471870:three_prime_UTR 40.0%
GAGAGAGAAAAGAAACTCAC+TGG + chr3_3:74246947-74246966 None:intergenic 40.0%
! GGCTAAATGGCATGTGTTTT+GGG - chr3_3:74247087-74247106 Msa0471870:CDS 40.0%
! AGGAGTTGTGTTTAGGGTTT+AGG - chr3_3:74247213-74247232 Msa0471870:CDS 40.0%
! GGAGTTGTGTTTAGGGTTTA+GGG - chr3_3:74247214-74247233 Msa0471870:CDS 40.0%
! GTGTTTAGGGTTTAGGGTTT+AGG - chr3_3:74247220-74247239 Msa0471870:CDS 40.0%
ATACTTGCCAAAGTCTGTCA+TGG + chr3_3:74247495-74247514 None:intergenic 40.0%
! CTAAATTGAGACATGGTGGA+TGG - chr3_3:74247587-74247606 Msa0471870:intron 40.0%
TGGTACTAAGTGTGGTTTGA+AGG - chr3_3:74247610-74247629 Msa0471870:intron 40.0%
GGTACTAAGTGTGGTTTGAA+GGG - chr3_3:74247611-74247630 Msa0471870:intron 40.0%
TATCACTCAACAAGGGATGT+AGG - chr3_3:74247642-74247661 Msa0471870:intron 40.0%
GCTACAAGTAGAAGAAGGAA+AGG + chr3_3:74247795-74247814 None:intergenic 40.0%
TAGTGGCTACAAGTAGAAGA+AGG + chr3_3:74247800-74247819 None:intergenic 40.0%
CAAAGGCTGCACAAACAAAA+TGG + chr3_3:74247889-74247908 None:intergenic 40.0%
!! CATTTTGTTTGTGCAGCCTT+TGG - chr3_3:74247887-74247906 Msa0471870:intron 40.0%
TAAACACCGGGTATACACAA+AGG - chr3_3:74247933-74247952 Msa0471870:intron 40.0%
ATGAAAGAAGCAAAGACAGG+TGG + chr3_3:74248040-74248059 None:intergenic 40.0%
TGCTCTGTCCAATTCCAAAA+TGG + chr3_3:74248236-74248255 None:intergenic 40.0%
TCAAACCTTCGTTCTCTGAA+GGG + chr3_3:74248317-74248336 None:intergenic 40.0%
AAACCAGTGTTGTCAAACAG+CGG + chr3_3:74248507-74248526 None:intergenic 40.0%
CTACACAGAGATTCATTGGT+CGG + chr3_3:74248711-74248730 None:intergenic 40.0%
AATCTCTGTGTAGGATAGGA+TGG - chr3_3:74248718-74248737 Msa0471870:intron 40.0%
CCTATAAAATAAGGCCAGAG+GGG - chr3_3:74248776-74248795 Msa0471870:intron 40.0%
CCTAAAATTCATTTCCCCTC+TGG + chr3_3:74248793-74248812 None:intergenic 40.0%
! CCAGAGGGGAAATGAATTTT+AGG - chr3_3:74248790-74248809 Msa0471870:intron 40.0%
ATCGTGATGTAAGCATCAAC+AGG + chr3_3:74249028-74249047 None:intergenic 40.0%
TGCTTACATCACGATTCTCT+TGG - chr3_3:74249033-74249052 Msa0471870:intron 40.0%
! TGATCTCATGTTACTTGGCA+TGG - chr3_3:74249202-74249221 Msa0471870:intron 40.0%
! GATCTCATGTTACTTGGCAT+GGG - chr3_3:74249203-74249222 Msa0471870:intron 40.0%
ATGTAGCTTCTTTGTTCCCA+GGG - chr3_3:74249237-74249256 Msa0471870:intron 40.0%
! TTTTGGTGAGTCGTTAATGG+AGG + chr3_3:74249297-74249316 None:intergenic 40.0%
!!! TGGTTTTGGTGAGTCGTTAA+TGG + chr3_3:74249300-74249319 None:intergenic 40.0%
AATGTTATTCTCTCTGGTGG+TGG + chr3_3:74249320-74249339 None:intergenic 40.0%
AGATGAATTGATCACGGGAA+AGG + chr3_3:74249345-74249364 None:intergenic 40.0%
GCCAGTTAATCTTTGTGTTG+TGG - chr3_3:74249537-74249556 Msa0471870:CDS 40.0%
ATCTCAGTGATAGTTGACAC+TGG - chr3_3:74249625-74249644 Msa0471870:CDS 40.0%
GTGGTCTTTGTAGCAATAAG+AGG - chr3_3:74249651-74249670 Msa0471870:CDS 40.0%
! AGTGCAATGCGAAGTTCTTT+TGG - chr3_3:74249689-74249708 Msa0471870:CDS 40.0%
AATACGAAAGATGAGGGGTA+GGG + chr3_3:74249770-74249789 None:intergenic 40.0%
! AACTAAGCAGTGGACTTAAG+TGG - chr3_3:74249873-74249892 Msa0471870:five_prime_UTR 40.0%
!!! CGCTTCACTTTTTGGTTTGG+TGG - chr3_3:74246545-74246564 Msa0471870:three_prime_UTR 45.0%
TAACCAAGAGTGGGTGAAAG+TGG + chr3_3:74246628-74246647 None:intergenic 45.0%
AAAGGGAAAGCGATGCGATT+CGG + chr3_3:74246665-74246684 None:intergenic 45.0%
! GACGCAAGTTTGGGTTTGAA+AGG + chr3_3:74246683-74246702 None:intergenic 45.0%
CTGAAAGTGGACGCAAGTTT+GGG + chr3_3:74246692-74246711 None:intergenic 45.0%
GGTGACACCTTTGAATCTGA+AGG + chr3_3:74246733-74246752 None:intergenic 45.0%
!! CCGTTTGTTGTTGTGTTTGG+CGG + chr3_3:74246754-74246773 None:intergenic 45.0%
CCGCCAAACACAACAACAAA+CGG - chr3_3:74246751-74246770 Msa0471870:three_prime_UTR 45.0%
CAACAAACGGTTCGTTTGCA+AGG - chr3_3:74246764-74246783 Msa0471870:three_prime_UTR 45.0%
! GGGCTAAATGGCATGTGTTT+TGG - chr3_3:74247086-74247105 Msa0471870:CDS 45.0%
GTAGAAACAGTCAGTATGCG+AGG - chr3_3:74247467-74247486 Msa0471870:intron 45.0%
!! AATTGAGACATGGTGGATGG+TGG - chr3_3:74247590-74247609 Msa0471870:intron 45.0%
CTGAAACACACTTTCTAGGC+TGG + chr3_3:74247740-74247759 None:intergenic 45.0%
! CAGCCTAGAAAGTGTGTTTC+AGG - chr3_3:74247738-74247757 Msa0471870:intron 45.0%
GCACCTGAAACACACTTTCT+AGG + chr3_3:74247744-74247763 None:intergenic 45.0%
TGCAGCCTTTGGTCAAGATT+TGG - chr3_3:74247898-74247917 Msa0471870:intron 45.0%
GGAAGCCAAATCTTGACCAA+AGG + chr3_3:74247906-74247925 None:intergenic 45.0%
GCTTCCTGATGCTTAAACAC+CGG - chr3_3:74247920-74247939 Msa0471870:intron 45.0%
CTTCCTGATGCTTAAACACC+GGG - chr3_3:74247921-74247940 Msa0471870:intron 45.0%
ATACCCGGTGTTTAAGCATC+AGG + chr3_3:74247927-74247946 None:intergenic 45.0%
!! ATTCTGAAGCACCAGCTTTC+TGG - chr3_3:74247977-74247996 Msa0471870:intron 45.0%
AAGTGACCTGCAAGATGTTG+AGG - chr3_3:74248280-74248299 Msa0471870:intron 45.0%
AGTGACCTGCAAGATGTTGA+GGG - chr3_3:74248281-74248300 Msa0471870:intron 45.0%
CTCAAACCTTCGTTCTCTGA+AGG + chr3_3:74248318-74248337 None:intergenic 45.0%
GCGATCCGTGATTGACAAAT+TGG + chr3_3:74248341-74248360 None:intergenic 45.0%
!! GACAACACTGGTTTGAGCAA+TGG - chr3_3:74248513-74248532 Msa0471870:intron 45.0%
CAATGGAAGACTCCACTTGT+GGG - chr3_3:74248530-74248549 Msa0471870:intron 45.0%
CGACCAATGAATCTCTGTGT+AGG - chr3_3:74248709-74248728 Msa0471870:intron 45.0%
!!! CCCCTCTGGCCTTATTTTAT+AGG + chr3_3:74248779-74248798 None:intergenic 45.0%
TACATCACGATTCTCTTGGC+AGG - chr3_3:74249037-74249056 Msa0471870:intron 45.0%
AATGATGCCCTTTCAGCTGA+GGG - chr3_3:74249092-74249111 Msa0471870:intron 45.0%
GACACATGTCTCGTAATCGT+CGG + chr3_3:74249123-74249142 None:intergenic 45.0%
CGAGACATGTGTCAAACACT+TGG - chr3_3:74249130-74249149 Msa0471870:intron 45.0%
CATGTAGCTTCTTTGTTCCC+AGG - chr3_3:74249236-74249255 Msa0471870:intron 45.0%
!!! ATTCTCTCTGGTGGTGGTTT+TGG + chr3_3:74249314-74249333 None:intergenic 45.0%
ATCTGCTTACATCGCTCTTG+TGG - chr3_3:74249361-74249380 Msa0471870:CDS 45.0%
TGAAAAGCTTCCTGCTGCAT+TGG - chr3_3:74249442-74249461 Msa0471870:intron 45.0%
!! CTGCATTGGTTTCACCTGAA+GGG - chr3_3:74249456-74249475 Msa0471870:intron 45.0%
!! TGCATTGGTTTCACCTGAAG+GGG - chr3_3:74249457-74249476 Msa0471870:intron 45.0%
AACCATTTCAACTCCCCTTC+AGG + chr3_3:74249473-74249492 None:intergenic 45.0%
AGGGGAGTTGAAATGGTTCT+TGG - chr3_3:74249475-74249494 Msa0471870:intron 45.0%
TGATAGTTGACACTGGACAG+TGG - chr3_3:74249632-74249651 Msa0471870:CDS 45.0%
GAATACGAAAGATGAGGGGT+AGG + chr3_3:74249771-74249790 None:intergenic 45.0%
TTCAGTTGAGATGCTTCCAG+CGG - chr3_3:74249793-74249812 Msa0471870:CDS 45.0%
!!! ATTTTTTTTTATGTTTTTTA+AGG - chr3_3:74247193-74247212 Msa0471870:CDS 5.0%
CTTCCACTTTCACCCACTCT+TGG - chr3_3:74246622-74246641 Msa0471870:three_prime_UTR 50.0%
AAGGGAAAGCGATGCGATTC+GGG + chr3_3:74246664-74246683 None:intergenic 50.0%
GCTGAAAGTGGACGCAAGTT+TGG + chr3_3:74246693-74246712 None:intergenic 50.0%
CAAACGGTTCGTTTGCAAGG+TGG - chr3_3:74246767-74246786 Msa0471870:three_prime_UTR 50.0%
CGAGGATCCATGACAGACTT+TGG - chr3_3:74247485-74247504 Msa0471870:intron 50.0%
! GTGGATGGTGGTACTAAGTG+TGG - chr3_3:74247602-74247621 Msa0471870:intron 50.0%
CACTCAACAAGGGATGTAGG+TGG - chr3_3:74247645-74247664 Msa0471870:intron 50.0%
AGGGATGTAGGTGGAAAGCA+TGG - chr3_3:74247654-74247673 Msa0471870:intron 50.0%
ACACTTTCTAGGCTGGACTG+GGG + chr3_3:74247733-74247752 None:intergenic 50.0%
CACACTTTCTAGGCTGGACT+GGG + chr3_3:74247734-74247753 None:intergenic 50.0%
ACACACTTTCTAGGCTGGAC+TGG + chr3_3:74247735-74247754 None:intergenic 50.0%
ACTTATGGTGCTCAAGTGCC+AGG - chr3_3:74247847-74247866 Msa0471870:intron 50.0%
GGCATTTGTTTCGCAGTACC+TGG + chr3_3:74247868-74247887 None:intergenic 50.0%
! GTTCTCTGAAGGGCATACCA+CGG + chr3_3:74248307-74248326 None:intergenic 50.0%
GTATGCCCTTCAGAGAACGA+AGG - chr3_3:74248309-74248328 Msa0471870:intron 50.0%
TTGACAAACGGCTGCCATTG+CGG + chr3_3:74248412-74248431 None:intergenic 50.0%
TAGCCGCTGTTTGACAACAC+TGG - chr3_3:74248501-74248520 Msa0471870:intron 50.0%
GCAATGGAAGACTCCACTTG+TGG - chr3_3:74248529-74248548 Msa0471870:intron 50.0%
CAATGATGCCCTTTCAGCTG+AGG - chr3_3:74249091-74249110 Msa0471870:intron 50.0%
AAGAAGCTACATGCCCGTCA+GGG + chr3_3:74249229-74249248 None:intergenic 50.0%
AAAGAAGCTACATGCCCGTC+AGG + chr3_3:74249230-74249249 None:intergenic 50.0%
TACATCGCTCTTGTGGTGAC+TGG - chr3_3:74249368-74249387 Msa0471870:CDS 50.0%
GTGAACTGCATCCGCTTTAC+CGG + chr3_3:74249396-74249415 None:intergenic 50.0%
TCAGGTGAAACCAATGCAGC+AGG + chr3_3:74249455-74249474 None:intergenic 50.0%
!! GCTGCATTGGTTTCACCTGA+AGG - chr3_3:74249455-74249474 Msa0471870:intron 50.0%
CACCTGAAGGGGAGTTGAAA+TGG - chr3_3:74249468-74249487 Msa0471870:intron 50.0%
AGGGAAAGCGATGCGATTCG+GGG + chr3_3:74246663-74246682 None:intergenic 55.0%
! GCTGGACTGGGGAAGCTTAA+AGG + chr3_3:74247722-74247741 None:intergenic 55.0%
! AAGACTCCACTTGTGGGCTG+TGG - chr3_3:74248536-74248555 Msa0471870:intron 55.0%
TTACTTGGCATGGGCCCTGA+CGG - chr3_3:74249212-74249231 Msa0471870:intron 55.0%
GCTCTTGTGGTGACTGGTAC+CGG - chr3_3:74249374-74249393 Msa0471870:CDS 55.0%
!! GGTGACTGGTACCGGTAAAG+CGG - chr3_3:74249382-74249401 Msa0471870:CDS 55.0%
GCAGTTCACTCTGCACTACG+TGG - chr3_3:74249407-74249426 Msa0471870:intron 55.0%
AACGTAACTCAGGCTTCCGC+TGG + chr3_3:74249812-74249831 None:intergenic 55.0%
CACGGCCCTCAACATCTTGC+AGG + chr3_3:74248289-74248308 None:intergenic 60.0%
CTGCAAGATGTTGAGGGCCG+TGG - chr3_3:74248287-74248306 Msa0471870:intron 60.0%
GTTACGCCACAGCCCACAAG+TGG + chr3_3:74248545-74248564 None:intergenic 60.0%
GGCAGCTCCCTCAGCTGAAA+GGG + chr3_3:74249102-74249121 None:intergenic 60.0%
TACTTGGCATGGGCCCTGAC+GGG - chr3_3:74249213-74249232 Msa0471870:intron 60.0%
ACCGCGCTATAGCGCCGCAA+TGG - chr3_3:74248395-74248414 Msa0471870:intron 65.0%
GCCATTGCGGCGCTATAGCG+CGG + chr3_3:74248399-74248418 None:intergenic 65.0%
TGGCAGGTGCCCATTCCACC+TGG - chr3_3:74249053-74249072 Msa0471870:intron 65.0%
CGGCAGCTCCCTCAGCTGAA+AGG + chr3_3:74249103-74249122 None:intergenic 65.0%
!! CGTGCACAAGAGGGTGCCCT+GGG + chr3_3:74249256-74249275 None:intergenic 65.0%
!! TCGTGCACAAGAGGGTGCCC+TGG + chr3_3:74249257-74249276 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 74246534 74249926 74246534 ID=Msa0471870;Name=Msa0471870
chr3_3 mRNA 74246534 74249926 74246534 ID=Msa0471870-mRNA-1;Parent=Msa0471870;Name=Msa0471870-mRNA-1;_AED=0.02;_eAED=0.02;_QI=84|1|1|1|1|1|3|403|309
chr3_3 exon 74246534 74247401 74246534 ID=Msa0471870-mRNA-1:exon:18948;Parent=Msa0471870-mRNA-1
chr3_3 exon 74249277 74249412 74249277 ID=Msa0471870-mRNA-1:exon:18947;Parent=Msa0471870-mRNA-1
chr3_3 exon 74249514 74249926 74249514 ID=Msa0471870-mRNA-1:exon:18946;Parent=Msa0471870-mRNA-1
chr3_3 five_prime_UTR 74249843 74249926 74249843 ID=Msa0471870-mRNA-1:five_prime_utr;Parent=Msa0471870-mRNA-1
chr3_3 CDS 74249514 74249842 74249514 ID=Msa0471870-mRNA-1:cds;Parent=Msa0471870-mRNA-1
chr3_3 CDS 74249277 74249412 74249277 ID=Msa0471870-mRNA-1:cds;Parent=Msa0471870-mRNA-1
chr3_3 CDS 74246937 74247401 74246937 ID=Msa0471870-mRNA-1:cds;Parent=Msa0471870-mRNA-1
chr3_3 three_prime_UTR 74246534 74246936 74246534 ID=Msa0471870-mRNA-1:three_prime_utr;Parent=Msa0471870-mRNA-1
Gene Sequence

>Msa0471870

ATGTCTTCCACTTTCACCCACTCTTGGTTACATCTCAACACTCCCCGAATCGCATCGCTTTCCCTTTCAAACCCAAACTTGCGTCCACTTTCAGCTCAATTCTCTTTTCGACCCTTCAGATTCAAAGGTGTCACCGCCAAACACAACAACAAACGGTTCGTTTGCAAGGTGGTGAAATCTTCAATGGCTGTTGAAGTTGAAGTCTTTGAAAAAGAACAACTCGCTGTTTCTCTCGCCAAATATGTCGCTGATCTCTCTAATAAGTTCACTCGCGAAAGAGGCGCTTTCACTGTTTGTTTATCTGGTGGCTCTTTGATCAACTATCTCCAGAAACTGTTGGAAGATCCGTATGTTGATTCTATTGAATGGGCTAAATGGCATGTGTTTTGGGTTGATGAGAGAGTTGTACCAAAGACTCATGATGATAGTAACTATAAGCTTGCTTTTGATGGGTTTCTATCCAAGGTGCCCATTCCACCTGGTAATGTTTATGCAATCAATGATGCCCTTTCAGCTGAGGGAGCTGCCGACGATTACGAGACATGTGTCAAACACTTGGTCAATAGCAATGTGATAACTTCATCATCAACCAGTGGATTTCCAAAATTTGATCTCATGTTACTTGGCATGGGCCCTGACGGGCATGTAGCTTCTTTGTTCCCAGGGCACCCTCTTGTGCACGAGAATAAAAAATGGGTTACCTCCATTAACGACTCACCAAAACCACCACCAGAGAGAATAACATTTACCTTTCCCGTGATCAATTCATCTGCTTACATCGCTCTTGTGGTGACTGGTACCGGTAAAGCGGATGCAGTTCACTCTGCACTACGTGGATCTGAAACTTCTGAAAAGCTTCCTGCTGCATTGGTTTCACCTGAAGGGGAGTTGAAATGGTTCTTGGACAAAGGTGCAGCGTCAAAGCTGTAG
Protein sequence

>Msa0471870

MSSTFTHSWLHLNTPRIASLSLSNPNLRPLSAQFSFRPFRFKGVTAKHNNKRFVCKVVKSSMAVEVEVFEKEQLAVSLAKYVADLSNKFTRERGAFTVCLSGGSLINYLQKLLEDPYVDSIEWAKWHVFWVDERVVPKTHDDSNYKLAFDGFLSKVPIPPGNVYAINDALSAEGAADDYETCVKHLVNSNVITSSSTSGFPKFDLMLLGMGPDGHVASLFPGHPLVHENKKWVTSINDSPKPPPERITFTFPVINSSAYIALVVTGTGKADAVHSALRGSETSEKLPAALVSPEGELKWFLDKGAASKL*