Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0478120 | CAB65283.1 | 82.051 | 117 | 6 | 2 | 1 | 102 | 1 | 117 | 1.22e-56 | 181 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0478120 | sp|Q02921|NO93_SOYBN | 64.583 | 48 | 17 | 0 | 45 | 92 | 50 | 97 | 2.81e-16 | 71.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0478120 | Q9SBR6 | 82.051 | 117 | 6 | 2 | 1 | 102 | 1 | 117 | 5.83e-57 | 181 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0478120 | Msa0478180 | 0.982758 | 6.351811e-156 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0478120 | MtrunA17_Chr3g0140601 | 80.342 | 117 | 8 | 2 | 1 | 102 | 1 | 117 | 2.47e-58 | 175 |
| Msa0478120 | MtrunA17_Chr1g0146471 | 54.701 | 117 | 36 | 3 | 1 | 102 | 1 | 115 | 6.36e-39 | 126 |
| Msa0478120 | MtrunA17_Chr4g0063331 | 68.750 | 48 | 15 | 0 | 45 | 92 | 54 | 101 | 3.74e-17 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0478120 | AT5G25940.1 | 70.213 | 47 | 14 | 0 | 45 | 91 | 57 | 103 | 1.83e-18 | 74.3 |
Find 18 sgRNAs with CRISPR-Local
Find 104 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTGCTGACAAAACTATCT+TGG | 0.397147 | 3_3:-82589133 | Msa0478120:CDS |
| TCAGAGACTCTTCTAGCAGC+AGG | 0.453559 | 3_3:+82589091 | None:intergenic |
| AACATCATCTGCTCTTCTGC+AGG | 0.462479 | 3_3:+82590244 | None:intergenic |
| TTGCAGTTCGGATGATTCCA+TGG | 0.467990 | 3_3:-82589720 | Msa0478120:CDS |
| ATTCACAGTTGGTTGCAGTT+CGG | 0.494528 | 3_3:-82589732 | Msa0478120:intron |
| AAACCTCAATTATACTGCTC+AGG | 0.499730 | 3_3:-82589689 | Msa0478120:CDS |
| CAGGCGCTTATCATATCTGC+TGG | 0.500846 | 3_3:-82589670 | Msa0478120:intron |
| CACATGTCCCTCAGTTCTGA+TGG | 0.505127 | 3_3:+82590304 | None:intergenic |
| TTGAGGTTTGCCTTTGCCCA+TGG | 0.506394 | 3_3:+82589703 | None:intergenic |
| AGGTCTAGTCAATGTACAGC+TGG | 0.529239 | 3_3:-82590220 | Msa0478120:CDS |
| GCGCCTGAGCAGTATAATTG+AGG | 0.541209 | 3_3:+82589686 | None:intergenic |
| CAGAGACTCTTCTAGCAGCA+GGG | 0.568654 | 3_3:+82589092 | None:intergenic |
| TGCAGTTCGGATGATTCCAT+GGG | 0.575792 | 3_3:-82589719 | Msa0478120:CDS |
| CATCAGAACTGAGGGACATG+TGG | 0.592369 | 3_3:-82590303 | Msa0478120:CDS |
| GGGAATTCCATCAGAACTGA+GGG | 0.594629 | 3_3:-82590311 | Msa0478120:CDS |
| TCGGATGATTCCATGGGCAA+AGG | 0.603271 | 3_3:-82589713 | Msa0478120:CDS |
| CAGAAGAGCAGATGATGTTG+AGG | 0.615138 | 3_3:-82590240 | Msa0478120:CDS |
| TGGGAATTCCATCAGAACTG+AGG | 0.659138 | 3_3:-82590312 | Msa0478120:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTTTTACATCTTTTT+TGG | - | chr3_3:82589471-82589490 | Msa0478120:intron | 10.0% |
| !!! | TTCTGTATTTTTTTTTTTTT+TGG | - | chr3_3:82589573-82589592 | Msa0478120:intron | 10.0% |
| !!! | TTTATTCTTAATTTTTTGAA+AGG | - | chr3_3:82590062-82590081 | Msa0478120:intron | 10.0% |
| !!! | TTTTTTTTTTACATCTTTTT+TGG | - | chr3_3:82589471-82589490 | Msa0478120:intron | 10.0% |
| !!! | TTCTGTATTTTTTTTTTTTT+TGG | - | chr3_3:82589573-82589592 | Msa0478120:intron | 10.0% |
| !!! | TTTATTCTTAATTTTTTGAA+AGG | - | chr3_3:82590062-82590081 | Msa0478120:intron | 10.0% |
| !!! | ATATTTCATCAATTGTTTTT+TGG | - | chr3_3:82589195-82589214 | Msa0478120:intron | 15.0% |
| !! | ATGAATAAGAAAATTAAAAC+TGG | + | chr3_3:82589261-82589280 | None:intergenic | 15.0% |
| !! | ATATATAGTGTGTATAAAAT+TGG | - | chr3_3:82589382-82589401 | Msa0478120:intron | 15.0% |
| !!! | ATAATTTCTTTAAGGATATA+AGG | + | chr3_3:82589442-82589461 | None:intergenic | 15.0% |
| !!! | AACAAACTTTAAGAAAATTA+TGG | - | chr3_3:82589498-82589517 | Msa0478120:intron | 15.0% |
| !!! | ATAATTTCTTTAAGGATATA+AGG | + | chr3_3:82589442-82589461 | None:intergenic | 15.0% |
| !! | TTACTCCTATTAATTAAATT+TGG | + | chr3_3:82589839-82589858 | None:intergenic | 15.0% |
| !!! | TGATTAATGAATTTTATCTA+AGG | + | chr3_3:82590009-82590028 | None:intergenic | 15.0% |
| !!! | TTCTAATTTTGATAATTTAG+TGG | - | chr3_3:82590190-82590209 | Msa0478120:intron | 15.0% |
| !!! | ATATTTCATCAATTGTTTTT+TGG | - | chr3_3:82589195-82589214 | Msa0478120:intron | 15.0% |
| !! | ATGAATAAGAAAATTAAAAC+TGG | + | chr3_3:82589261-82589280 | None:intergenic | 15.0% |
| !! | ATATATAGTGTGTATAAAAT+TGG | - | chr3_3:82589382-82589401 | Msa0478120:intron | 15.0% |
| !!! | ATAATTTCTTTAAGGATATA+AGG | + | chr3_3:82589442-82589461 | None:intergenic | 15.0% |
| !!! | AACAAACTTTAAGAAAATTA+TGG | - | chr3_3:82589498-82589517 | Msa0478120:intron | 15.0% |
| !!! | ATAATTTCTTTAAGGATATA+AGG | + | chr3_3:82589442-82589461 | None:intergenic | 15.0% |
| !! | TTACTCCTATTAATTAAATT+TGG | + | chr3_3:82589839-82589858 | None:intergenic | 15.0% |
| !!! | TGATTAATGAATTTTATCTA+AGG | + | chr3_3:82590009-82590028 | None:intergenic | 15.0% |
| !!! | TTCTAATTTTGATAATTTAG+TGG | - | chr3_3:82590190-82590209 | Msa0478120:intron | 15.0% |
| !! | TACAGAACATAATTTCTTTA+AGG | + | chr3_3:82589450-82589469 | None:intergenic | 20.0% |
| !! | TACAGAACATAATTTCTTTA+AGG | + | chr3_3:82589450-82589469 | None:intergenic | 20.0% |
| !! | ATTAAGAATAAGTTGTAACT+TGG | + | chr3_3:82590118-82590137 | None:intergenic | 20.0% |
| !!! | TTTGATAATTTAGTGGTTTT+TGG | - | chr3_3:82590197-82590216 | Msa0478120:intron | 20.0% |
| !! | TACAGAACATAATTTCTTTA+AGG | + | chr3_3:82589450-82589469 | None:intergenic | 20.0% |
| !! | TACAGAACATAATTTCTTTA+AGG | + | chr3_3:82589450-82589469 | None:intergenic | 20.0% |
| !! | ATTAAGAATAAGTTGTAACT+TGG | + | chr3_3:82590118-82590137 | None:intergenic | 20.0% |
| !!! | TTTGATAATTTAGTGGTTTT+TGG | - | chr3_3:82590197-82590216 | Msa0478120:intron | 20.0% |
| ! | TAGCACAAATGTAACATATA+AGG | + | chr3_3:82589613-82589632 | None:intergenic | 25.0% |
| ! | TGCCACCAAATTTAATTAAT+AGG | - | chr3_3:82589831-82589850 | Msa0478120:intron | 25.0% |
| ! | CTCCTATTAATTAAATTTGG+TGG | + | chr3_3:82589836-82589855 | None:intergenic | 25.0% |
| ! | AACTTGTAAACCAAGTAATA+GGG | + | chr3_3:82590038-82590057 | None:intergenic | 25.0% |
| !! | TAACTTGTAAACCAAGTAAT+AGG | + | chr3_3:82590039-82590058 | None:intergenic | 25.0% |
| ! | TAGCACAAATGTAACATATA+AGG | + | chr3_3:82589613-82589632 | None:intergenic | 25.0% |
| ! | TGCCACCAAATTTAATTAAT+AGG | - | chr3_3:82589831-82589850 | Msa0478120:intron | 25.0% |
| ! | CTCCTATTAATTAAATTTGG+TGG | + | chr3_3:82589836-82589855 | None:intergenic | 25.0% |
| ! | AACTTGTAAACCAAGTAATA+GGG | + | chr3_3:82590038-82590057 | None:intergenic | 25.0% |
| !! | TAACTTGTAAACCAAGTAAT+AGG | + | chr3_3:82590039-82590058 | None:intergenic | 25.0% |
| !! | CAGCAAACTTGTGTTTTTAT+TGG | + | chr3_3:82589117-82589136 | None:intergenic | 30.0% |
| !!! | CATCAATTGTTTTTTGGCAA+TGG | - | chr3_3:82589201-82589220 | Msa0478120:intron | 30.0% |
| AGGATATAAGGACATTCATA+TGG | + | chr3_3:82589430-82589449 | None:intergenic | 30.0% | |
| AGGATATAAGGACATTCATA+TGG | + | chr3_3:82589430-82589449 | None:intergenic | 30.0% | |
| AGCTTTACAAAATTCACAGT+TGG | - | chr3_3:82589647-82589666 | Msa0478120:intron | 30.0% | |
| TAGCTAACAATTTGATTCCA+CGG | - | chr3_3:82589901-82589920 | Msa0478120:intron | 30.0% | |
| !! | CAGCAAACTTGTGTTTTTAT+TGG | + | chr3_3:82589117-82589136 | None:intergenic | 30.0% |
| !!! | CATCAATTGTTTTTTGGCAA+TGG | - | chr3_3:82589201-82589220 | Msa0478120:intron | 30.0% |
| AGGATATAAGGACATTCATA+TGG | + | chr3_3:82589430-82589449 | None:intergenic | 30.0% | |
| AGGATATAAGGACATTCATA+TGG | + | chr3_3:82589430-82589449 | None:intergenic | 30.0% | |
| AGCTTTACAAAATTCACAGT+TGG | - | chr3_3:82589647-82589666 | Msa0478120:intron | 30.0% | |
| TAGCTAACAATTTGATTCCA+CGG | - | chr3_3:82589901-82589920 | Msa0478120:intron | 30.0% | |
| TGTTTGACTAAGATCCTTGT+TGG | + | chr3_3:82589229-82589248 | None:intergenic | 35.0% | |
| AAACCTCAATTATACTGCTC+AGG | - | chr3_3:82589701-82589720 | Msa0478120:CDS | 35.0% | |
| ATCACTTGAACCCTATTACT+TGG | - | chr3_3:82590025-82590044 | Msa0478120:intron | 35.0% | |
| TGTTTGACTAAGATCCTTGT+TGG | + | chr3_3:82589229-82589248 | None:intergenic | 35.0% | |
| AAACCTCAATTATACTGCTC+AGG | - | chr3_3:82589701-82589720 | Msa0478120:CDS | 35.0% | |
| ATCACTTGAACCCTATTACT+TGG | - | chr3_3:82590025-82590044 | Msa0478120:intron | 35.0% | |
| ! | TTTTGGCAATGGATCCAACA+AGG | - | chr3_3:82589212-82589231 | Msa0478120:intron | 40.0% |
| ATTCACAGTTGGTTGCAGTT+CGG | - | chr3_3:82589658-82589677 | Msa0478120:intron | 40.0% | |
| CTAACAATTTGATTCCACGG+AGG | - | chr3_3:82589904-82589923 | Msa0478120:intron | 40.0% | |
| ATTTGATTCCACGGAGGATA+TGG | - | chr3_3:82589910-82589929 | Msa0478120:intron | 40.0% | |
| GACTCAACTAATCGAGAGTT+TGG | - | chr3_3:82589932-82589951 | Msa0478120:intron | 40.0% | |
| TGCTGCTGACAAAACTATCT+TGG | - | chr3_3:82590257-82590276 | Msa0478120:CDS | 40.0% | |
| ! | TTTTGGCAATGGATCCAACA+AGG | - | chr3_3:82589212-82589231 | Msa0478120:intron | 40.0% |
| ATTCACAGTTGGTTGCAGTT+CGG | - | chr3_3:82589658-82589677 | Msa0478120:intron | 40.0% | |
| CTAACAATTTGATTCCACGG+AGG | - | chr3_3:82589904-82589923 | Msa0478120:intron | 40.0% | |
| ATTTGATTCCACGGAGGATA+TGG | - | chr3_3:82589910-82589929 | Msa0478120:intron | 40.0% | |
| GACTCAACTAATCGAGAGTT+TGG | - | chr3_3:82589932-82589951 | Msa0478120:intron | 40.0% | |
| TGCTGCTGACAAAACTATCT+TGG | - | chr3_3:82590257-82590276 | Msa0478120:CDS | 40.0% | |
| TGGGAATTCCATCAGAACTG+AGG | - | chr3_3:82589078-82589097 | Msa0478120:CDS | 45.0% | |
| GGGAATTCCATCAGAACTGA+GGG | - | chr3_3:82589079-82589098 | Msa0478120:CDS | 45.0% | |
| AACATCATCTGCTCTTCTGC+AGG | + | chr3_3:82589149-82589168 | None:intergenic | 45.0% | |
| CAGAAGAGCAGATGATGTTG+AGG | - | chr3_3:82589150-82589169 | Msa0478120:CDS | 45.0% | |
| AGGTCTAGTCAATGTACAGC+TGG | - | chr3_3:82589170-82589189 | Msa0478120:intron | 45.0% | |
| TTGCAGTTCGGATGATTCCA+TGG | - | chr3_3:82589670-82589689 | Msa0478120:intron | 45.0% | |
| TGCAGTTCGGATGATTCCAT+GGG | - | chr3_3:82589671-82589690 | Msa0478120:CDS | 45.0% | |
| TGGGAATTCCATCAGAACTG+AGG | - | chr3_3:82589078-82589097 | Msa0478120:CDS | 45.0% | |
| GGGAATTCCATCAGAACTGA+GGG | - | chr3_3:82589079-82589098 | Msa0478120:CDS | 45.0% | |
| AACATCATCTGCTCTTCTGC+AGG | + | chr3_3:82589149-82589168 | None:intergenic | 45.0% | |
| CAGAAGAGCAGATGATGTTG+AGG | - | chr3_3:82589150-82589169 | Msa0478120:CDS | 45.0% | |
| AGGTCTAGTCAATGTACAGC+TGG | - | chr3_3:82589170-82589189 | Msa0478120:intron | 45.0% | |
| TTGCAGTTCGGATGATTCCA+TGG | - | chr3_3:82589670-82589689 | Msa0478120:intron | 45.0% | |
| TGCAGTTCGGATGATTCCAT+GGG | - | chr3_3:82589671-82589690 | Msa0478120:CDS | 45.0% | |
| ! | CACATGTCCCTCAGTTCTGA+TGG | + | chr3_3:82589089-82589108 | None:intergenic | 50.0% |
| CATCAGAACTGAGGGACATG+TGG | - | chr3_3:82589087-82589106 | Msa0478120:CDS | 50.0% | |
| TCGGATGATTCCATGGGCAA+AGG | - | chr3_3:82589677-82589696 | Msa0478120:CDS | 50.0% | |
| TTGAGGTTTGCCTTTGCCCA+TGG | + | chr3_3:82589690-82589709 | None:intergenic | 50.0% | |
| GCGCCTGAGCAGTATAATTG+AGG | + | chr3_3:82589707-82589726 | None:intergenic | 50.0% | |
| CAGGCGCTTATCATATCTGC+TGG | - | chr3_3:82589720-82589739 | Msa0478120:CDS | 50.0% | |
| AGTTGAGTCCATATCCTCCG+TGG | + | chr3_3:82589921-82589940 | None:intergenic | 50.0% | |
| CAGAGACTCTTCTAGCAGCA+GGG | + | chr3_3:82590301-82590320 | None:intergenic | 50.0% | |
| TCAGAGACTCTTCTAGCAGC+AGG | + | chr3_3:82590302-82590321 | None:intergenic | 50.0% | |
| ! | CACATGTCCCTCAGTTCTGA+TGG | + | chr3_3:82589089-82589108 | None:intergenic | 50.0% |
| CATCAGAACTGAGGGACATG+TGG | - | chr3_3:82589087-82589106 | Msa0478120:CDS | 50.0% | |
| TCGGATGATTCCATGGGCAA+AGG | - | chr3_3:82589677-82589696 | Msa0478120:CDS | 50.0% | |
| TTGAGGTTTGCCTTTGCCCA+TGG | + | chr3_3:82589690-82589709 | None:intergenic | 50.0% | |
| GCGCCTGAGCAGTATAATTG+AGG | + | chr3_3:82589707-82589726 | None:intergenic | 50.0% | |
| CAGGCGCTTATCATATCTGC+TGG | - | chr3_3:82589720-82589739 | Msa0478120:CDS | 50.0% | |
| AGTTGAGTCCATATCCTCCG+TGG | + | chr3_3:82589921-82589940 | None:intergenic | 50.0% | |
| CAGAGACTCTTCTAGCAGCA+GGG | + | chr3_3:82590301-82590320 | None:intergenic | 50.0% | |
| TCAGAGACTCTTCTAGCAGC+AGG | + | chr3_3:82590302-82590321 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_3 | gene | 82589077 | 82590335 | 82589077 | ID=Msa0478120;Name=Msa0478120 |
| chr3_3 | mRNA | 82589077 | 82590335 | 82589077 | ID=Msa0478120-mRNA-1;Parent=Msa0478120;Name=Msa0478120-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0|0|1|0.5|0.33|3|0|102 |
| chr3_3 | exon | 82590204 | 82590335 | 82590204 | ID=Msa0478120-mRNA-1:exon:23132;Parent=Msa0478120-mRNA-1 |
| chr3_3 | exon | 82589671 | 82589746 | 82589671 | ID=Msa0478120-mRNA-1:exon:23131;Parent=Msa0478120-mRNA-1 |
| chr3_3 | exon | 82589077 | 82589177 | 82589077 | ID=Msa0478120-mRNA-1:exon:23130;Parent=Msa0478120-mRNA-1 |
| chr3_3 | CDS | 82590204 | 82590335 | 82590204 | ID=Msa0478120-mRNA-1:cds;Parent=Msa0478120-mRNA-1 |
| chr3_3 | CDS | 82589671 | 82589746 | 82589671 | ID=Msa0478120-mRNA-1:cds;Parent=Msa0478120-mRNA-1 |
| chr3_3 | CDS | 82589077 | 82589177 | 82589077 | ID=Msa0478120-mRNA-1:cds;Parent=Msa0478120-mRNA-1 |
| Gene Sequence |
| Protein sequence |
