Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0478180 | CAB65283.1 | 82.051 | 117 | 6 | 2 | 1 | 102 | 1 | 117 | 1.22e-56 | 181 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0478180 | sp|Q02921|NO93_SOYBN | 64.583 | 48 | 17 | 0 | 45 | 92 | 50 | 97 | 2.81e-16 | 71.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0478180 | Q9SBR6 | 82.051 | 117 | 6 | 2 | 1 | 102 | 1 | 117 | 5.83e-57 | 181 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0478120 | Msa0478180 | 0.982758 | 6.351811e-156 | -8.615850e-47 |
| Msa0478180 | Msa0904590 | 0.801906 | 7.218728e-49 | -8.615850e-47 |
| Msa0478180 | Msa0958830 | 0.800003 | 1.767102e-48 | -8.615850e-47 |
| Msa0320170 | Msa0478180 | 0.809364 | 1.962547e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0478180 | MtrunA17_Chr3g0140601 | 80.342 | 117 | 8 | 2 | 1 | 102 | 1 | 117 | 2.47e-58 | 175 |
| Msa0478180 | MtrunA17_Chr1g0146471 | 54.701 | 117 | 36 | 3 | 1 | 102 | 1 | 115 | 6.36e-39 | 126 |
| Msa0478180 | MtrunA17_Chr4g0063331 | 68.750 | 48 | 15 | 0 | 45 | 92 | 54 | 101 | 3.74e-17 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0478180 | AT5G25940.1 | 70.213 | 47 | 14 | 0 | 45 | 91 | 57 | 103 | 1.83e-18 | 74.3 |
Find 18 sgRNAs with CRISPR-Local
Find 104 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTGCTGACAAAACTATCT+TGG | 0.397147 | 3_3:-82633393 | Msa0478180:CDS |
| TCAGAGACTCTTCTAGCAGC+AGG | 0.453559 | 3_3:+82633351 | None:intergenic |
| AACATCATCTGCTCTTCTGC+AGG | 0.462479 | 3_3:+82634503 | None:intergenic |
| TTGCAGTTCGGATGATTCCA+TGG | 0.467990 | 3_3:-82633980 | Msa0478180:CDS |
| ATTCACAGTTGGTTGCAGTT+CGG | 0.494528 | 3_3:-82633992 | Msa0478180:intron |
| AAACCTCAATTATACTGCTC+AGG | 0.499730 | 3_3:-82633949 | Msa0478180:CDS |
| CAGGCGCTTATCATATCTGC+TGG | 0.500846 | 3_3:-82633930 | Msa0478180:intron |
| CACATGTCCCTCAGTTCTGA+TGG | 0.505127 | 3_3:+82634563 | None:intergenic |
| TTGAGGTTTGCCTTTGCCCA+TGG | 0.506394 | 3_3:+82633963 | None:intergenic |
| AGGTCTAGTCAATGTACAGC+TGG | 0.529239 | 3_3:-82634479 | Msa0478180:CDS |
| GCGCCTGAGCAGTATAATTG+AGG | 0.541209 | 3_3:+82633946 | None:intergenic |
| CAGAGACTCTTCTAGCAGCA+GGG | 0.568654 | 3_3:+82633352 | None:intergenic |
| TGCAGTTCGGATGATTCCAT+GGG | 0.575792 | 3_3:-82633979 | Msa0478180:CDS |
| CATCAGAACTGAGGGACATG+TGG | 0.592369 | 3_3:-82634562 | Msa0478180:CDS |
| GGGAATTCCATCAGAACTGA+GGG | 0.594629 | 3_3:-82634570 | Msa0478180:CDS |
| TCGGATGATTCCATGGGCAA+AGG | 0.603271 | 3_3:-82633973 | Msa0478180:CDS |
| CAGAAGAGCAGATGATGTTG+AGG | 0.615138 | 3_3:-82634499 | Msa0478180:CDS |
| TGGGAATTCCATCAGAACTG+AGG | 0.659138 | 3_3:-82634571 | Msa0478180:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTTTTACATCTTTTT+TGG | - | chr3_3:82633731-82633750 | Msa0478180:intron | 10.0% |
| !!! | TTTATTCTTAATTTTTTGAA+AGG | - | chr3_3:82634321-82634340 | Msa0478180:intron | 10.0% |
| !!! | TTTTTTTTTTACATCTTTTT+TGG | - | chr3_3:82633731-82633750 | Msa0478180:intron | 10.0% |
| !!! | TTTATTCTTAATTTTTTGAA+AGG | - | chr3_3:82634321-82634340 | Msa0478180:intron | 10.0% |
| !!! | ATATTTCATCAATTGTTTTT+TGG | - | chr3_3:82633455-82633474 | Msa0478180:intron | 15.0% |
| !! | ATGAATAAGAAAATTAAAAC+TGG | + | chr3_3:82633521-82633540 | None:intergenic | 15.0% |
| !! | ATATATAGTGTGTATAAAAT+TGG | - | chr3_3:82633642-82633661 | Msa0478180:intron | 15.0% |
| !!! | ATAATTTCTTTAAGGATATA+AGG | + | chr3_3:82633702-82633721 | None:intergenic | 15.0% |
| !!! | AACAAACTTTAAGAAAATTA+TGG | - | chr3_3:82633758-82633777 | Msa0478180:intron | 15.0% |
| !!! | ATAATTTCTTTAAGGATATA+AGG | + | chr3_3:82633702-82633721 | None:intergenic | 15.0% |
| !!! | GTTCTGTATTTTTTTTTTTT+TGG | - | chr3_3:82633832-82633851 | Msa0478180:intron | 15.0% |
| !! | TTACTCCTATTAATTAAATT+TGG | + | chr3_3:82634098-82634117 | None:intergenic | 15.0% |
| !!! | TGATTAATGAATTTTATCTA+AGG | + | chr3_3:82634268-82634287 | None:intergenic | 15.0% |
| !!! | TTCTAATTTTGATAATTTAG+TGG | - | chr3_3:82634449-82634468 | Msa0478180:intron | 15.0% |
| !!! | ATATTTCATCAATTGTTTTT+TGG | - | chr3_3:82633455-82633474 | Msa0478180:intron | 15.0% |
| !! | ATGAATAAGAAAATTAAAAC+TGG | + | chr3_3:82633521-82633540 | None:intergenic | 15.0% |
| !! | ATATATAGTGTGTATAAAAT+TGG | - | chr3_3:82633642-82633661 | Msa0478180:intron | 15.0% |
| !!! | ATAATTTCTTTAAGGATATA+AGG | + | chr3_3:82633702-82633721 | None:intergenic | 15.0% |
| !!! | AACAAACTTTAAGAAAATTA+TGG | - | chr3_3:82633758-82633777 | Msa0478180:intron | 15.0% |
| !!! | ATAATTTCTTTAAGGATATA+AGG | + | chr3_3:82633702-82633721 | None:intergenic | 15.0% |
| !!! | GTTCTGTATTTTTTTTTTTT+TGG | - | chr3_3:82633832-82633851 | Msa0478180:intron | 15.0% |
| !! | TTACTCCTATTAATTAAATT+TGG | + | chr3_3:82634098-82634117 | None:intergenic | 15.0% |
| !!! | TGATTAATGAATTTTATCTA+AGG | + | chr3_3:82634268-82634287 | None:intergenic | 15.0% |
| !!! | TTCTAATTTTGATAATTTAG+TGG | - | chr3_3:82634449-82634468 | Msa0478180:intron | 15.0% |
| !! | TACAGAACATAATTTCTTTA+AGG | + | chr3_3:82633710-82633729 | None:intergenic | 20.0% |
| !! | TACAGAACATAATTTCTTTA+AGG | + | chr3_3:82633710-82633729 | None:intergenic | 20.0% |
| !! | ATTAAGAATAAGTTGTAACT+TGG | + | chr3_3:82634377-82634396 | None:intergenic | 20.0% |
| !!! | TTTGATAATTTAGTGGTTTT+TGG | - | chr3_3:82634456-82634475 | Msa0478180:intron | 20.0% |
| !! | TACAGAACATAATTTCTTTA+AGG | + | chr3_3:82633710-82633729 | None:intergenic | 20.0% |
| !! | TACAGAACATAATTTCTTTA+AGG | + | chr3_3:82633710-82633729 | None:intergenic | 20.0% |
| !! | ATTAAGAATAAGTTGTAACT+TGG | + | chr3_3:82634377-82634396 | None:intergenic | 20.0% |
| !!! | TTTGATAATTTAGTGGTTTT+TGG | - | chr3_3:82634456-82634475 | Msa0478180:intron | 20.0% |
| ! | TAGCACAAATGTAACATATA+AGG | + | chr3_3:82633872-82633891 | None:intergenic | 25.0% |
| ! | TGCCACCAAATTTAATTAAT+AGG | - | chr3_3:82634090-82634109 | Msa0478180:intron | 25.0% |
| ! | CTCCTATTAATTAAATTTGG+TGG | + | chr3_3:82634095-82634114 | None:intergenic | 25.0% |
| ! | AACTTGTAAACCAAGTAATA+GGG | + | chr3_3:82634297-82634316 | None:intergenic | 25.0% |
| !! | TAACTTGTAAACCAAGTAAT+AGG | + | chr3_3:82634298-82634317 | None:intergenic | 25.0% |
| ! | TAGCACAAATGTAACATATA+AGG | + | chr3_3:82633872-82633891 | None:intergenic | 25.0% |
| ! | TGCCACCAAATTTAATTAAT+AGG | - | chr3_3:82634090-82634109 | Msa0478180:intron | 25.0% |
| ! | CTCCTATTAATTAAATTTGG+TGG | + | chr3_3:82634095-82634114 | None:intergenic | 25.0% |
| ! | AACTTGTAAACCAAGTAATA+GGG | + | chr3_3:82634297-82634316 | None:intergenic | 25.0% |
| !! | TAACTTGTAAACCAAGTAAT+AGG | + | chr3_3:82634298-82634317 | None:intergenic | 25.0% |
| !! | CAGCAAACTTGTGTTTTTAT+TGG | + | chr3_3:82633377-82633396 | None:intergenic | 30.0% |
| !!! | CATCAATTGTTTTTTGGCAA+TGG | - | chr3_3:82633461-82633480 | Msa0478180:intron | 30.0% |
| AGGATATAAGGACATTCATA+TGG | + | chr3_3:82633690-82633709 | None:intergenic | 30.0% | |
| AGGATATAAGGACATTCATA+TGG | + | chr3_3:82633690-82633709 | None:intergenic | 30.0% | |
| AGCTTTACAAAATTCACAGT+TGG | - | chr3_3:82633906-82633925 | Msa0478180:intron | 30.0% | |
| TAGCTAACAATTTGATTCCA+CGG | - | chr3_3:82634160-82634179 | Msa0478180:intron | 30.0% | |
| !! | CAGCAAACTTGTGTTTTTAT+TGG | + | chr3_3:82633377-82633396 | None:intergenic | 30.0% |
| !!! | CATCAATTGTTTTTTGGCAA+TGG | - | chr3_3:82633461-82633480 | Msa0478180:intron | 30.0% |
| AGGATATAAGGACATTCATA+TGG | + | chr3_3:82633690-82633709 | None:intergenic | 30.0% | |
| AGGATATAAGGACATTCATA+TGG | + | chr3_3:82633690-82633709 | None:intergenic | 30.0% | |
| AGCTTTACAAAATTCACAGT+TGG | - | chr3_3:82633906-82633925 | Msa0478180:intron | 30.0% | |
| TAGCTAACAATTTGATTCCA+CGG | - | chr3_3:82634160-82634179 | Msa0478180:intron | 30.0% | |
| TGTTTGACTAAGATCCTTGT+TGG | + | chr3_3:82633489-82633508 | None:intergenic | 35.0% | |
| AAACCTCAATTATACTGCTC+AGG | - | chr3_3:82633960-82633979 | Msa0478180:CDS | 35.0% | |
| ATCACTTGAACCCTATTACT+TGG | - | chr3_3:82634284-82634303 | Msa0478180:intron | 35.0% | |
| TGTTTGACTAAGATCCTTGT+TGG | + | chr3_3:82633489-82633508 | None:intergenic | 35.0% | |
| AAACCTCAATTATACTGCTC+AGG | - | chr3_3:82633960-82633979 | Msa0478180:CDS | 35.0% | |
| ATCACTTGAACCCTATTACT+TGG | - | chr3_3:82634284-82634303 | Msa0478180:intron | 35.0% | |
| ! | TTTTGGCAATGGATCCAACA+AGG | - | chr3_3:82633472-82633491 | Msa0478180:intron | 40.0% |
| ATTCACAGTTGGTTGCAGTT+CGG | - | chr3_3:82633917-82633936 | Msa0478180:intron | 40.0% | |
| CTAACAATTTGATTCCACGG+AGG | - | chr3_3:82634163-82634182 | Msa0478180:intron | 40.0% | |
| ATTTGATTCCACGGAGGATA+TGG | - | chr3_3:82634169-82634188 | Msa0478180:intron | 40.0% | |
| GACTCAACTAATCGAGAGTT+TGG | - | chr3_3:82634191-82634210 | Msa0478180:intron | 40.0% | |
| TGCTGCTGACAAAACTATCT+TGG | - | chr3_3:82634516-82634535 | Msa0478180:CDS | 40.0% | |
| ! | TTTTGGCAATGGATCCAACA+AGG | - | chr3_3:82633472-82633491 | Msa0478180:intron | 40.0% |
| ATTCACAGTTGGTTGCAGTT+CGG | - | chr3_3:82633917-82633936 | Msa0478180:intron | 40.0% | |
| CTAACAATTTGATTCCACGG+AGG | - | chr3_3:82634163-82634182 | Msa0478180:intron | 40.0% | |
| ATTTGATTCCACGGAGGATA+TGG | - | chr3_3:82634169-82634188 | Msa0478180:intron | 40.0% | |
| GACTCAACTAATCGAGAGTT+TGG | - | chr3_3:82634191-82634210 | Msa0478180:intron | 40.0% | |
| TGCTGCTGACAAAACTATCT+TGG | - | chr3_3:82634516-82634535 | Msa0478180:CDS | 40.0% | |
| TGGGAATTCCATCAGAACTG+AGG | - | chr3_3:82633338-82633357 | Msa0478180:CDS | 45.0% | |
| GGGAATTCCATCAGAACTGA+GGG | - | chr3_3:82633339-82633358 | Msa0478180:CDS | 45.0% | |
| AACATCATCTGCTCTTCTGC+AGG | + | chr3_3:82633409-82633428 | None:intergenic | 45.0% | |
| CAGAAGAGCAGATGATGTTG+AGG | - | chr3_3:82633410-82633429 | Msa0478180:CDS | 45.0% | |
| AGGTCTAGTCAATGTACAGC+TGG | - | chr3_3:82633430-82633449 | Msa0478180:intron | 45.0% | |
| TTGCAGTTCGGATGATTCCA+TGG | - | chr3_3:82633929-82633948 | Msa0478180:intron | 45.0% | |
| TGCAGTTCGGATGATTCCAT+GGG | - | chr3_3:82633930-82633949 | Msa0478180:intron | 45.0% | |
| TGGGAATTCCATCAGAACTG+AGG | - | chr3_3:82633338-82633357 | Msa0478180:CDS | 45.0% | |
| GGGAATTCCATCAGAACTGA+GGG | - | chr3_3:82633339-82633358 | Msa0478180:CDS | 45.0% | |
| AACATCATCTGCTCTTCTGC+AGG | + | chr3_3:82633409-82633428 | None:intergenic | 45.0% | |
| CAGAAGAGCAGATGATGTTG+AGG | - | chr3_3:82633410-82633429 | Msa0478180:CDS | 45.0% | |
| AGGTCTAGTCAATGTACAGC+TGG | - | chr3_3:82633430-82633449 | Msa0478180:intron | 45.0% | |
| TTGCAGTTCGGATGATTCCA+TGG | - | chr3_3:82633929-82633948 | Msa0478180:intron | 45.0% | |
| TGCAGTTCGGATGATTCCAT+GGG | - | chr3_3:82633930-82633949 | Msa0478180:intron | 45.0% | |
| ! | CACATGTCCCTCAGTTCTGA+TGG | + | chr3_3:82633349-82633368 | None:intergenic | 50.0% |
| CATCAGAACTGAGGGACATG+TGG | - | chr3_3:82633347-82633366 | Msa0478180:CDS | 50.0% | |
| TCGGATGATTCCATGGGCAA+AGG | - | chr3_3:82633936-82633955 | Msa0478180:CDS | 50.0% | |
| TTGAGGTTTGCCTTTGCCCA+TGG | + | chr3_3:82633949-82633968 | None:intergenic | 50.0% | |
| GCGCCTGAGCAGTATAATTG+AGG | + | chr3_3:82633966-82633985 | None:intergenic | 50.0% | |
| CAGGCGCTTATCATATCTGC+TGG | - | chr3_3:82633979-82633998 | Msa0478180:CDS | 50.0% | |
| AGTTGAGTCCATATCCTCCG+TGG | + | chr3_3:82634180-82634199 | None:intergenic | 50.0% | |
| CAGAGACTCTTCTAGCAGCA+GGG | + | chr3_3:82634560-82634579 | None:intergenic | 50.0% | |
| TCAGAGACTCTTCTAGCAGC+AGG | + | chr3_3:82634561-82634580 | None:intergenic | 50.0% | |
| ! | CACATGTCCCTCAGTTCTGA+TGG | + | chr3_3:82633349-82633368 | None:intergenic | 50.0% |
| CATCAGAACTGAGGGACATG+TGG | - | chr3_3:82633347-82633366 | Msa0478180:CDS | 50.0% | |
| TCGGATGATTCCATGGGCAA+AGG | - | chr3_3:82633936-82633955 | Msa0478180:CDS | 50.0% | |
| TTGAGGTTTGCCTTTGCCCA+TGG | + | chr3_3:82633949-82633968 | None:intergenic | 50.0% | |
| GCGCCTGAGCAGTATAATTG+AGG | + | chr3_3:82633966-82633985 | None:intergenic | 50.0% | |
| CAGGCGCTTATCATATCTGC+TGG | - | chr3_3:82633979-82633998 | Msa0478180:CDS | 50.0% | |
| AGTTGAGTCCATATCCTCCG+TGG | + | chr3_3:82634180-82634199 | None:intergenic | 50.0% | |
| CAGAGACTCTTCTAGCAGCA+GGG | + | chr3_3:82634560-82634579 | None:intergenic | 50.0% | |
| TCAGAGACTCTTCTAGCAGC+AGG | + | chr3_3:82634561-82634580 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_3 | gene | 82633337 | 82634594 | 82633337 | ID=Msa0478180;Name=Msa0478180 |
| chr3_3 | mRNA | 82633337 | 82634594 | 82633337 | ID=Msa0478180-mRNA-1;Parent=Msa0478180;Name=Msa0478180-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0|0|1|0.5|0.33|3|0|102 |
| chr3_3 | exon | 82634463 | 82634594 | 82634463 | ID=Msa0478180-mRNA-1:exon:23135;Parent=Msa0478180-mRNA-1 |
| chr3_3 | exon | 82633931 | 82634006 | 82633931 | ID=Msa0478180-mRNA-1:exon:23134;Parent=Msa0478180-mRNA-1 |
| chr3_3 | exon | 82633337 | 82633437 | 82633337 | ID=Msa0478180-mRNA-1:exon:23133;Parent=Msa0478180-mRNA-1 |
| chr3_3 | CDS | 82634463 | 82634594 | 82634463 | ID=Msa0478180-mRNA-1:cds;Parent=Msa0478180-mRNA-1 |
| chr3_3 | CDS | 82633931 | 82634006 | 82633931 | ID=Msa0478180-mRNA-1:cds;Parent=Msa0478180-mRNA-1 |
| chr3_3 | CDS | 82633337 | 82633437 | 82633337 | ID=Msa0478180-mRNA-1:cds;Parent=Msa0478180-mRNA-1 |
| Gene Sequence |
| Protein sequence |
