AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0478790

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0478790 MtrunA17_Chr3g0141651 97.561 205 5 0 1 205 1 205 8.08e-150 414
Msa0478790 MtrunA17_Chr5g0433161 47.596 208 102 4 1 204 1 205 1.39e-57 180
Msa0478790 MtrunA17_Chr5g0433101 38.983 118 62 4 1 113 1 113 2.83e-15 73.6
Msa0478790 MtrunA17_Chr8g0384891 31.061 132 84 3 79 205 233 362 4.99e-14 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0478790 AT1G47271.1 61.494 174 64 3 33 205 22 193 4.64e-72 217
Msa0478790 AT5G10860.1 45.631 206 110 2 1 204 1 206 2.01e-58 183
Msa0478790 AT2G36500.1 32.323 99 63 3 81 178 79 174 2.90e-11 62.4

Find 71 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCTCGCCGTAACCCTTTA+AGG 0.117601 3_3:-83651383 Msa0478790:CDS
TACAATGGCATATTCTATTT+TGG 0.168795 3_3:+83649972 None:intergenic
GCTGCAATTCTTAGTGATTT+TGG 0.174322 3_3:+83649917 None:intergenic
TGAAATATTGCAGCCCTTAA+AGG 0.204426 3_3:+83651369 None:intergenic
CCTGCTATTTGCTGGCCTTC+TGG 0.215101 3_3:+83651011 None:intergenic
ATCAAAATTTAAATTATTGA+TGG 0.292779 3_3:+83651463 None:intergenic
ACATTAGTCTCATTGCTCTT+AGG 0.295642 3_3:+83650294 None:intergenic
AAGAAAGAACCGAGGGTATA+TGG 0.299405 3_3:+83651526 None:intergenic
TAGATGGCTGAAAATGATAT+TGG 0.310617 3_3:-83651053 Msa0478790:CDS
AACAAGTTATCTTCAGGCTT+TGG 0.326784 3_3:-83651315 Msa0478790:CDS
CGTCATGTTCCAGTTATAGA+TGG 0.340768 3_3:-83650165 Msa0478790:CDS
GGGAAGAAAAGGTGGGTTCC+TGG 0.355107 3_3:-83651215 Msa0478790:CDS
CATCAGAGGAGAATACTATT+AGG 0.368680 3_3:-83650055 Msa0478790:exon
TTTCAACAATTCCACGGTAA+TGG 0.371480 3_3:-83651351 Msa0478790:CDS
TTCCACGGTAATGGAATGTT+TGG 0.375408 3_3:-83651342 Msa0478790:CDS
ACCCATGCTGCAGAAGGGTT+TGG 0.378937 3_3:-83651274 Msa0478790:CDS
TTTAGAAATATTCTCGTGTA+TGG 0.380917 3_3:+83649895 None:intergenic
AAAAGGTGGGTTCCTGGCTT+TGG 0.383695 3_3:-83651209 Msa0478790:CDS
GAAATATTGCAGCCCTTAAA+GGG 0.387071 3_3:+83651370 None:intergenic
TGTCCAACTTGTGTGTATAA+AGG 0.398768 3_3:+83650460 None:intergenic
CAACATCAAATCTGTACATA+TGG 0.408404 3_3:+83650008 None:intergenic
TGACAATGCCTGCTATTTGC+TGG 0.409790 3_3:+83651003 None:intergenic
GGCCTGAACAAGTTATCTTC+AGG 0.414746 3_3:-83651321 Msa0478790:CDS
AGCCTGAAGATAACTTGTTC+AGG 0.435588 3_3:+83651319 None:intergenic
TCTTAGGATGTTTGTGTCAG+AGG 0.462266 3_3:+83650310 None:intergenic
TCCAAACCCTTCTGCAGCAT+GGG 0.467098 3_3:+83651273 None:intergenic
TTGCAGCCCTTAAAGGGTTA+CGG 0.469636 3_3:+83651376 None:intergenic
TGAAAATGATATTGGATCAT+TGG 0.473949 3_3:-83651045 Msa0478790:CDS
CTCCAAACCCTTCTGCAGCA+TGG 0.476764 3_3:+83651272 None:intergenic
CTCTCCACCCATGCTGCAGA+AGG 0.479958 3_3:-83651280 Msa0478790:CDS
TACGGCGAGGTTGCAGTATC+CGG 0.480241 3_3:+83651394 None:intergenic
TTATCTTCAGGCTTTGGATG+TGG 0.480771 3_3:-83651309 Msa0478790:CDS
AGAGCAATGAGACTAATGTT+AGG 0.488222 3_3:-83650290 Msa0478790:intron
CCAGAAGGCCAGCAAATAGC+AGG 0.489371 3_3:-83651011 Msa0478790:CDS
AGCAGGCATTGTCACAGAAA+GGG 0.492285 3_3:-83650994 Msa0478790:CDS
ACCTCGCCGTAACCCTTTAA+GGG 0.493422 3_3:-83651382 Msa0478790:CDS
GTACATATGGATGGTTGAAT+CGG 0.496072 3_3:+83650021 None:intergenic
TCAGAGGTACTAATGACAAA+GGG 0.503836 3_3:-83651237 Msa0478790:CDS
TGTCAGAGGTCACAGTTACC+AGG 0.516292 3_3:+83650324 None:intergenic
TAGCAGGCATTGTCACAGAA+AGG 0.525200 3_3:-83650995 Msa0478790:CDS
TCACCTTTATACACACAAGT+TGG 0.528416 3_3:-83650463 Msa0478790:CDS
AATACTATTAGGCTTGCAAA+AGG 0.533961 3_3:-83650044 Msa0478790:exon
AAACCCTTCTGCAGCATGGG+TGG 0.534547 3_3:+83651276 None:intergenic
TGGACAAATAATGACTAATG+AGG 0.572173 3_3:-83650443 Msa0478790:intron
ATCAAATCTGTACATATGGA+TGG 0.572235 3_3:+83650012 None:intergenic
GTTATAGATGGGAAAATAGT+CGG 0.577116 3_3:-83650153 Msa0478790:CDS
ATCGACTCTACACCAAAGCC+AGG 0.577827 3_3:+83651197 None:intergenic
GTCATGTTCCAGTTATAGAT+GGG 0.585549 3_3:-83650164 Msa0478790:CDS
ACTATGAAATACAAATTGCA+AGG 0.587651 3_3:-83651560 Msa0478790:five_prime_UTR
ATCAGAGGTACTAATGACAA+AGG 0.592971 3_3:-83651238 Msa0478790:CDS
GCAGGCATTGTCACAGAAAG+GGG 0.594143 3_3:-83650993 Msa0478790:intron
TTGAAGAAAATAGTTGCAGA+AGG 0.596178 3_3:-83650490 Msa0478790:CDS
CTTCTGCAGCATGGGTGGAG+AGG 0.598096 3_3:+83651281 None:intergenic
GGAGAATGTTACAGTATCAG+AGG 0.600301 3_3:-83651253 Msa0478790:CDS
GCAATATTTCAACAATTCCA+CGG 0.603864 3_3:-83651357 Msa0478790:CDS
GAATGTGAATTAGTATACAA+TGG 0.605783 3_3:+83649957 None:intergenic
TATGATTTCAATTGTAGATG+TGG 0.609194 3_3:-83650130 Msa0478790:CDS
CGACTCGGTGATTACATCAG+AGG 0.609575 3_3:-83650069 Msa0478790:CDS
GGCCAAACATTCCATTACCG+TGG 0.611771 3_3:+83651340 None:intergenic
GTACTAGTTCCATATACCCT+CGG 0.614997 3_3:-83651535 Msa0478790:five_prime_UTR
AAAATAAAAGAAAGAACCGA+GGG 0.616873 3_3:+83651519 None:intergenic
GCAGTGACAGAGCAACAAAG+TGG 0.618035 3_3:-83650102 Msa0478790:CDS
TCTCCACCCATGCTGCAGAA+GGG 0.621330 3_3:-83651279 Msa0478790:CDS
AAATGATATTGGATCATTGG+TGG 0.635058 3_3:-83651042 Msa0478790:CDS
AGTGGAGAATTGAAGCGACT+CGG 0.646159 3_3:-83650084 Msa0478790:CDS
CATCTATAACTGGAACATGA+CGG 0.646171 3_3:+83650166 None:intergenic
GCCCTTAAAGGGTTACGGCG+AGG 0.647884 3_3:+83651381 None:intergenic
TTGGTGGTACTGAAGCCAGA+AGG 0.655679 3_3:-83651026 Msa0478790:CDS
CAGAGGTACTAATGACAAAG+GGG 0.670481 3_3:-83651236 Msa0478790:CDS
AGAGGTACTAATGACAAAGG+GGG 0.700006 3_3:-83651235 Msa0478790:CDS
TTACCTATAACAGAATGACC+TGG 0.725918 3_3:-83650342 Msa0478790:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAAAATTTAAATTATTGA+TGG + chr3_3:83649901-83649920 None:intergenic 10.0%
!!! TTATAATTTGTATCAATATA+TGG - chr3_3:83650762-83650781 Msa0478790:intron 10.0%
!! ATCAAAATTTAAATTATTGA+TGG + chr3_3:83649901-83649920 None:intergenic 10.0%
!!! TTATAATTTGTATCAATATA+TGG - chr3_3:83650762-83650781 Msa0478790:intron 10.0%
!!! CAAATATATAATGTTTTAGA+TGG - chr3_3:83650292-83650311 Msa0478790:CDS 15.0%
!!! CAAATATATAATGTTTTAGA+TGG - chr3_3:83650292-83650311 Msa0478790:CDS 15.0%
!! TCAAGATAGAATTTATTATG+TGG - chr3_3:83650483-83650502 Msa0478790:CDS 20.0%
!! TCAAAATTCTAATAGTCAAT+AGG - chr3_3:83650526-83650545 Msa0478790:intron 20.0%
!!! ATTTGGTCACATAAATTTTA+TGG + chr3_3:83651125-83651144 None:intergenic 20.0%
!! TCAAGATAGAATTTATTATG+TGG - chr3_3:83650483-83650502 Msa0478790:CDS 20.0%
!! TCAAAATTCTAATAGTCAAT+AGG - chr3_3:83650526-83650545 Msa0478790:intron 20.0%
!!! ATTTGGTCACATAAATTTTA+TGG + chr3_3:83651125-83651144 None:intergenic 20.0%
! AAAATAAAAGAAAGAACCGA+GGG + chr3_3:83649845-83649864 None:intergenic 25.0%
! AAAAATAAAAGAAAGAACCG+AGG + chr3_3:83649846-83649865 None:intergenic 25.0%
!!! GGTTCTTTCTTTTATTTTTC+TGG - chr3_3:83649847-83649866 Msa0478790:three_prime_UTR 25.0%
! TGAAAATGATATTGGATCAT+TGG - chr3_3:83650316-83650335 Msa0478790:CDS 25.0%
! TAGAATTTATTATGTGGACA+CGG - chr3_3:83650489-83650508 Msa0478790:CDS 25.0%
!!! GAATTTTGATTTGATAACCA+TGG + chr3_3:83650515-83650534 None:intergenic 25.0%
! AATGACAAAAGAAAAGTAGT+TGG - chr3_3:83650612-83650631 Msa0478790:intron 25.0%
!! CTTTTCTTGTTCAAATTTGT+AGG + chr3_3:83650647-83650666 None:intergenic 25.0%
! CTACAAATTTGAACAAGAAA+AGG - chr3_3:83650645-83650664 Msa0478790:intron 25.0%
!!! ATAGCTTTATGAACAAAACA+AGG - chr3_3:83650808-83650827 Msa0478790:intron 25.0%
! ACACATGTTTCTTCAATATT+TGG + chr3_3:83651142-83651161 None:intergenic 25.0%
! TATGATTTCAATTGTAGATG+TGG - chr3_3:83651231-83651250 Msa0478790:CDS 25.0%
!! TACAATGGCATATTCTATTT+TGG + chr3_3:83651392-83651411 None:intergenic 25.0%
! GAATGTGAATTAGTATACAA+TGG + chr3_3:83651407-83651426 None:intergenic 25.0%
! TTTAGAAATATTCTCGTGTA+TGG + chr3_3:83651469-83651488 None:intergenic 25.0%
!! AACTGAGTTTTTGTCAATTA+TGG + chr3_3:83651508-83651527 None:intergenic 25.0%
! AAAATAAAAGAAAGAACCGA+GGG + chr3_3:83649845-83649864 None:intergenic 25.0%
! AAAAATAAAAGAAAGAACCG+AGG + chr3_3:83649846-83649865 None:intergenic 25.0%
!!! GGTTCTTTCTTTTATTTTTC+TGG - chr3_3:83649847-83649866 Msa0478790:three_prime_UTR 25.0%
! TGAAAATGATATTGGATCAT+TGG - chr3_3:83650316-83650335 Msa0478790:CDS 25.0%
! TAGAATTTATTATGTGGACA+CGG - chr3_3:83650489-83650508 Msa0478790:CDS 25.0%
!!! GAATTTTGATTTGATAACCA+TGG + chr3_3:83650515-83650534 None:intergenic 25.0%
! AATGACAAAAGAAAAGTAGT+TGG - chr3_3:83650612-83650631 Msa0478790:intron 25.0%
!! CTTTTCTTGTTCAAATTTGT+AGG + chr3_3:83650647-83650666 None:intergenic 25.0%
! CTACAAATTTGAACAAGAAA+AGG - chr3_3:83650645-83650664 Msa0478790:intron 25.0%
!!! ATAGCTTTATGAACAAAACA+AGG - chr3_3:83650808-83650827 Msa0478790:intron 25.0%
! ACACATGTTTCTTCAATATT+TGG + chr3_3:83651142-83651161 None:intergenic 25.0%
! TATGATTTCAATTGTAGATG+TGG - chr3_3:83651231-83651250 Msa0478790:CDS 25.0%
!! TACAATGGCATATTCTATTT+TGG + chr3_3:83651392-83651411 None:intergenic 25.0%
! GAATGTGAATTAGTATACAA+TGG + chr3_3:83651407-83651426 None:intergenic 25.0%
! TTTAGAAATATTCTCGTGTA+TGG + chr3_3:83651469-83651488 None:intergenic 25.0%
!! AACTGAGTTTTTGTCAATTA+TGG + chr3_3:83651508-83651527 None:intergenic 25.0%
!! TTATTTTTCTGGTGAAGCTT+TGG - chr3_3:83649858-83649877 Msa0478790:three_prime_UTR 30.0%
AGTGAACAACAAAAAATGCA+AGG - chr3_3:83649938-83649957 Msa0478790:three_prime_UTR 30.0%
AAAAAATGCAAGGAATCTAC+CGG - chr3_3:83649948-83649967 Msa0478790:three_prime_UTR 30.0%
GCAATATTTCAACAATTCCA+CGG - chr3_3:83650004-83650023 Msa0478790:three_prime_UTR 30.0%
TAGATGGCTGAAAATGATAT+TGG - chr3_3:83650308-83650327 Msa0478790:CDS 30.0%
AAATGATATTGGATCATTGG+TGG - chr3_3:83650319-83650338 Msa0478790:CDS 30.0%
TGTTTATCTCAGCAACTTAT+TGG + chr3_3:83650592-83650611 None:intergenic 30.0%
AAACAAGGTAAAATCTCTGT+TGG - chr3_3:83650823-83650842 Msa0478790:intron 30.0%
TTGAAGAAAATAGTTGCAGA+AGG - chr3_3:83650871-83650890 Msa0478790:intron 30.0%
TGGACAAATAATGACTAATG+AGG - chr3_3:83650918-83650937 Msa0478790:intron 30.0%
TTCTCTGCAATTCAAGTAAA+GGG + chr3_3:83651169-83651188 None:intergenic 30.0%
ATTCTCTGCAATTCAAGTAA+AGG + chr3_3:83651170-83651189 None:intergenic 30.0%
! ACTATTTTCCCATCTATAAC+TGG + chr3_3:83651208-83651227 None:intergenic 30.0%
GTTATAGATGGGAAAATAGT+CGG - chr3_3:83651208-83651227 Msa0478790:CDS 30.0%
AATACTATTAGGCTTGCAAA+AGG - chr3_3:83651317-83651336 Msa0478790:CDS 30.0%
ATCAAATCTGTACATATGGA+TGG + chr3_3:83651352-83651371 None:intergenic 30.0%
CAACATCAAATCTGTACATA+TGG + chr3_3:83651356-83651375 None:intergenic 30.0%
GACAAAAACTCAGTTATTTG+AGG - chr3_3:83651513-83651532 Msa0478790:five_prime_UTR 30.0%
TCCTTGCAATTTGTATTTCA+TGG + chr3_3:83649805-83649824 None:intergenic 30.0%
!! TTATTTTTCTGGTGAAGCTT+TGG - chr3_3:83649858-83649877 Msa0478790:three_prime_UTR 30.0%
AGTGAACAACAAAAAATGCA+AGG - chr3_3:83649938-83649957 Msa0478790:three_prime_UTR 30.0%
AAAAAATGCAAGGAATCTAC+CGG - chr3_3:83649948-83649967 Msa0478790:three_prime_UTR 30.0%
GCAATATTTCAACAATTCCA+CGG - chr3_3:83650004-83650023 Msa0478790:three_prime_UTR 30.0%
TAGATGGCTGAAAATGATAT+TGG - chr3_3:83650308-83650327 Msa0478790:CDS 30.0%
AAATGATATTGGATCATTGG+TGG - chr3_3:83650319-83650338 Msa0478790:CDS 30.0%
TGTTTATCTCAGCAACTTAT+TGG + chr3_3:83650592-83650611 None:intergenic 30.0%
AAACAAGGTAAAATCTCTGT+TGG - chr3_3:83650823-83650842 Msa0478790:intron 30.0%
TTGAAGAAAATAGTTGCAGA+AGG - chr3_3:83650871-83650890 Msa0478790:intron 30.0%
TGGACAAATAATGACTAATG+AGG - chr3_3:83650918-83650937 Msa0478790:intron 30.0%
TTCTCTGCAATTCAAGTAAA+GGG + chr3_3:83651169-83651188 None:intergenic 30.0%
ATTCTCTGCAATTCAAGTAA+AGG + chr3_3:83651170-83651189 None:intergenic 30.0%
! ACTATTTTCCCATCTATAAC+TGG + chr3_3:83651208-83651227 None:intergenic 30.0%
GTTATAGATGGGAAAATAGT+CGG - chr3_3:83651208-83651227 Msa0478790:CDS 30.0%
AATACTATTAGGCTTGCAAA+AGG - chr3_3:83651317-83651336 Msa0478790:CDS 30.0%
ATCAAATCTGTACATATGGA+TGG + chr3_3:83651352-83651371 None:intergenic 30.0%
CAACATCAAATCTGTACATA+TGG + chr3_3:83651356-83651375 None:intergenic 30.0%
GACAAAAACTCAGTTATTTG+AGG - chr3_3:83651513-83651532 Msa0478790:five_prime_UTR 30.0%
GAAATATTGCAGCCCTTAAA+GGG + chr3_3:83649994-83650013 None:intergenic 35.0%
TGAAATATTGCAGCCCTTAA+AGG + chr3_3:83649995-83650014 None:intergenic 35.0%
TTTCAACAATTCCACGGTAA+TGG - chr3_3:83650010-83650029 Msa0478790:three_prime_UTR 35.0%
AACAAGTTATCTTCAGGCTT+TGG - chr3_3:83650046-83650065 Msa0478790:exon 35.0%
!! ATCAGAGGTACTAATGACAA+AGG - chr3_3:83650123-83650142 Msa0478790:CDS 35.0%
!! TCAGAGGTACTAATGACAAA+GGG - chr3_3:83650124-83650143 Msa0478790:CDS 35.0%
TCACCTTTATACACACAAGT+TGG - chr3_3:83650898-83650917 Msa0478790:intron 35.0%
TGTCCAACTTGTGTGTATAA+AGG + chr3_3:83650904-83650923 None:intergenic 35.0%
TTACCTATAACAGAATGACC+TGG - chr3_3:83651019-83651038 Msa0478790:CDS 35.0%
TTACCAGGTCATTCTGTTAT+AGG + chr3_3:83651025-83651044 None:intergenic 35.0%
! ACATTAGTCTCATTGCTCTT+AGG + chr3_3:83651070-83651089 None:intergenic 35.0%
AGAGCAATGAGACTAATGTT+AGG - chr3_3:83651071-83651090 Msa0478790:intron 35.0%
CATCTATAACTGGAACATGA+CGG + chr3_3:83651198-83651217 None:intergenic 35.0%
GTCATGTTCCAGTTATAGAT+GGG - chr3_3:83651197-83651216 Msa0478790:CDS 35.0%
CATCAGAGGAGAATACTATT+AGG - chr3_3:83651306-83651325 Msa0478790:CDS 35.0%
GTACATATGGATGGTTGAAT+CGG + chr3_3:83651343-83651362 None:intergenic 35.0%
! GCTGCAATTCTTAGTGATTT+TGG + chr3_3:83651447-83651466 None:intergenic 35.0%
GAAATATTGCAGCCCTTAAA+GGG + chr3_3:83649994-83650013 None:intergenic 35.0%
TGAAATATTGCAGCCCTTAA+AGG + chr3_3:83649995-83650014 None:intergenic 35.0%
TTTCAACAATTCCACGGTAA+TGG - chr3_3:83650010-83650029 Msa0478790:three_prime_UTR 35.0%
AACAAGTTATCTTCAGGCTT+TGG - chr3_3:83650046-83650065 Msa0478790:exon 35.0%
!! ATCAGAGGTACTAATGACAA+AGG - chr3_3:83650123-83650142 Msa0478790:CDS 35.0%
!! TCAGAGGTACTAATGACAAA+GGG - chr3_3:83650124-83650143 Msa0478790:CDS 35.0%
TCACCTTTATACACACAAGT+TGG - chr3_3:83650898-83650917 Msa0478790:intron 35.0%
TGTCCAACTTGTGTGTATAA+AGG + chr3_3:83650904-83650923 None:intergenic 35.0%
TTACCTATAACAGAATGACC+TGG - chr3_3:83651019-83651038 Msa0478790:CDS 35.0%
TTACCAGGTCATTCTGTTAT+AGG + chr3_3:83651025-83651044 None:intergenic 35.0%
! ACATTAGTCTCATTGCTCTT+AGG + chr3_3:83651070-83651089 None:intergenic 35.0%
AGAGCAATGAGACTAATGTT+AGG - chr3_3:83651071-83651090 Msa0478790:intron 35.0%
CATCTATAACTGGAACATGA+CGG + chr3_3:83651198-83651217 None:intergenic 35.0%
GTCATGTTCCAGTTATAGAT+GGG - chr3_3:83651197-83651216 Msa0478790:CDS 35.0%
CATCAGAGGAGAATACTATT+AGG - chr3_3:83651306-83651325 Msa0478790:CDS 35.0%
GTACATATGGATGGTTGAAT+CGG + chr3_3:83651343-83651362 None:intergenic 35.0%
! GCTGCAATTCTTAGTGATTT+TGG + chr3_3:83651447-83651466 None:intergenic 35.0%
GTACTAGTTCCATATACCCT+CGG - chr3_3:83649826-83649845 Msa0478790:three_prime_UTR 40.0%
! AAGAAAGAACCGAGGGTATA+TGG + chr3_3:83649838-83649857 None:intergenic 40.0%
!! TTCCACGGTAATGGAATGTT+TGG - chr3_3:83650019-83650038 Msa0478790:three_prime_UTR 40.0%
!! AGCCTGAAGATAACTTGTTC+AGG + chr3_3:83650045-83650064 None:intergenic 40.0%
!! TTATCTTCAGGCTTTGGATG+TGG - chr3_3:83650052-83650071 Msa0478790:exon 40.0%
GGAGAATGTTACAGTATCAG+AGG - chr3_3:83650108-83650127 Msa0478790:CDS 40.0%
!! CAGAGGTACTAATGACAAAG+GGG - chr3_3:83650125-83650144 Msa0478790:CDS 40.0%
!! AGAGGTACTAATGACAAAGG+GGG - chr3_3:83650126-83650145 Msa0478790:CDS 40.0%
AATGACAAAGGGGGAAGAAA+AGG - chr3_3:83650135-83650154 Msa0478790:CDS 40.0%
GAGCAACGCATAATATGAGA+TGG + chr3_3:83650419-83650438 None:intergenic 40.0%
TCTTAGGATGTTTGTGTCAG+AGG + chr3_3:83651054-83651073 None:intergenic 40.0%
CGTCATGTTCCAGTTATAGA+TGG - chr3_3:83651196-83651215 Msa0478790:CDS 40.0%
!! GTGATTTTGGTCTCGTTCTT+AGG + chr3_3:83651434-83651453 None:intergenic 40.0%
GTACTAGTTCCATATACCCT+CGG - chr3_3:83649826-83649845 Msa0478790:three_prime_UTR 40.0%
! AAGAAAGAACCGAGGGTATA+TGG + chr3_3:83649838-83649857 None:intergenic 40.0%
!! TTCCACGGTAATGGAATGTT+TGG - chr3_3:83650019-83650038 Msa0478790:three_prime_UTR 40.0%
!! AGCCTGAAGATAACTTGTTC+AGG + chr3_3:83650045-83650064 None:intergenic 40.0%
!! TTATCTTCAGGCTTTGGATG+TGG - chr3_3:83650052-83650071 Msa0478790:exon 40.0%
GGAGAATGTTACAGTATCAG+AGG - chr3_3:83650108-83650127 Msa0478790:CDS 40.0%
!! CAGAGGTACTAATGACAAAG+GGG - chr3_3:83650125-83650144 Msa0478790:CDS 40.0%
!! AGAGGTACTAATGACAAAGG+GGG - chr3_3:83650126-83650145 Msa0478790:CDS 40.0%
AATGACAAAGGGGGAAGAAA+AGG - chr3_3:83650135-83650154 Msa0478790:CDS 40.0%
GAGCAACGCATAATATGAGA+TGG + chr3_3:83650419-83650438 None:intergenic 40.0%
TCTTAGGATGTTTGTGTCAG+AGG + chr3_3:83651054-83651073 None:intergenic 40.0%
CGTCATGTTCCAGTTATAGA+TGG - chr3_3:83651196-83651215 Msa0478790:CDS 40.0%
!! GTGATTTTGGTCTCGTTCTT+AGG + chr3_3:83651434-83651453 None:intergenic 40.0%
TTGCAGCCCTTAAAGGGTTA+CGG + chr3_3:83649988-83650007 None:intergenic 45.0%
GGCCTGAACAAGTTATCTTC+AGG - chr3_3:83650040-83650059 Msa0478790:exon 45.0%
ACAAAGGGGGAAGAAAAGGT+GGG - chr3_3:83650139-83650158 Msa0478790:CDS 45.0%
! TGACAATGCCTGCTATTTGC+TGG + chr3_3:83650361-83650380 None:intergenic 45.0%
! TAGCAGGCATTGTCACAGAA+AGG - chr3_3:83650366-83650385 Msa0478790:intron 45.0%
! AGCAGGCATTGTCACAGAAA+GGG - chr3_3:83650367-83650386 Msa0478790:intron 45.0%
!! GAGATGGAGAAAGTATCCGA+AGG + chr3_3:83650403-83650422 None:intergenic 45.0%
TTATTATGTGGACACGGCCA+TGG - chr3_3:83650495-83650514 Msa0478790:CDS 45.0%
GCAGTCTGTAGAATCATGCA+TGG + chr3_3:83650851-83650870 None:intergenic 45.0%
AGTGGAGAATTGAAGCGACT+CGG - chr3_3:83651277-83651296 Msa0478790:CDS 45.0%
TTGCAGCCCTTAAAGGGTTA+CGG + chr3_3:83649988-83650007 None:intergenic 45.0%
GGCCTGAACAAGTTATCTTC+AGG - chr3_3:83650040-83650059 Msa0478790:exon 45.0%
ACAAAGGGGGAAGAAAAGGT+GGG - chr3_3:83650139-83650158 Msa0478790:CDS 45.0%
! TGACAATGCCTGCTATTTGC+TGG + chr3_3:83650361-83650380 None:intergenic 45.0%
! TAGCAGGCATTGTCACAGAA+AGG - chr3_3:83650366-83650385 Msa0478790:intron 45.0%
! AGCAGGCATTGTCACAGAAA+GGG - chr3_3:83650367-83650386 Msa0478790:intron 45.0%
!! GAGATGGAGAAAGTATCCGA+AGG + chr3_3:83650403-83650422 None:intergenic 45.0%
TTATTATGTGGACACGGCCA+TGG - chr3_3:83650495-83650514 Msa0478790:CDS 45.0%
GCAGTCTGTAGAATCATGCA+TGG + chr3_3:83650851-83650870 None:intergenic 45.0%
AGTGGAGAATTGAAGCGACT+CGG - chr3_3:83651277-83651296 Msa0478790:CDS 45.0%
AACCTCGCCGTAACCCTTTA+AGG - chr3_3:83649978-83649997 Msa0478790:three_prime_UTR 50.0%
ACCTCGCCGTAACCCTTTAA+GGG - chr3_3:83649979-83649998 Msa0478790:three_prime_UTR 50.0%
GGCCAAACATTCCATTACCG+TGG + chr3_3:83650024-83650043 None:intergenic 50.0%
TCCAAACCCTTCTGCAGCAT+GGG + chr3_3:83650091-83650110 None:intergenic 50.0%
GACAAAGGGGGAAGAAAAGG+TGG - chr3_3:83650138-83650157 Msa0478790:CDS 50.0%
! AAAAGGTGGGTTCCTGGCTT+TGG - chr3_3:83650152-83650171 Msa0478790:CDS 50.0%
! ATCGACTCTACACCAAAGCC+AGG + chr3_3:83650167-83650186 None:intergenic 50.0%
! TTGGTGGTACTGAAGCCAGA+AGG - chr3_3:83650335-83650354 Msa0478790:intron 50.0%
! GCAGGCATTGTCACAGAAAG+GGG - chr3_3:83650368-83650387 Msa0478790:intron 50.0%
GCATTGTCACAGAAAGGGGT+AGG - chr3_3:83650372-83650391 Msa0478790:intron 50.0%
TTGTCACAGAAAGGGGTAGG+CGG - chr3_3:83650375-83650394 Msa0478790:intron 50.0%
TGTCAGAGGTCACAGTTACC+AGG + chr3_3:83651040-83651059 None:intergenic 50.0%
GCAGTGACAGAGCAACAAAG+TGG - chr3_3:83651259-83651278 Msa0478790:CDS 50.0%
!! CGACTCGGTGATTACATCAG+AGG - chr3_3:83651292-83651311 Msa0478790:CDS 50.0%
AACCTCGCCGTAACCCTTTA+AGG - chr3_3:83649978-83649997 Msa0478790:three_prime_UTR 50.0%
ACCTCGCCGTAACCCTTTAA+GGG - chr3_3:83649979-83649998 Msa0478790:three_prime_UTR 50.0%
GGCCAAACATTCCATTACCG+TGG + chr3_3:83650024-83650043 None:intergenic 50.0%
TCCAAACCCTTCTGCAGCAT+GGG + chr3_3:83650091-83650110 None:intergenic 50.0%
GACAAAGGGGGAAGAAAAGG+TGG - chr3_3:83650138-83650157 Msa0478790:CDS 50.0%
! AAAAGGTGGGTTCCTGGCTT+TGG - chr3_3:83650152-83650171 Msa0478790:CDS 50.0%
! ATCGACTCTACACCAAAGCC+AGG + chr3_3:83650167-83650186 None:intergenic 50.0%
! TTGGTGGTACTGAAGCCAGA+AGG - chr3_3:83650335-83650354 Msa0478790:intron 50.0%
! GCAGGCATTGTCACAGAAAG+GGG - chr3_3:83650368-83650387 Msa0478790:intron 50.0%
GCATTGTCACAGAAAGGGGT+AGG - chr3_3:83650372-83650391 Msa0478790:intron 50.0%
TTGTCACAGAAAGGGGTAGG+CGG - chr3_3:83650375-83650394 Msa0478790:intron 50.0%
TGTCAGAGGTCACAGTTACC+AGG + chr3_3:83651040-83651059 None:intergenic 50.0%
GCAGTGACAGAGCAACAAAG+TGG - chr3_3:83651259-83651278 Msa0478790:CDS 50.0%
!! CGACTCGGTGATTACATCAG+AGG - chr3_3:83651292-83651311 Msa0478790:CDS 50.0%
TACGGCGAGGTTGCAGTATC+CGG + chr3_3:83649970-83649989 None:intergenic 55.0%
TCTCCACCCATGCTGCAGAA+GGG - chr3_3:83650082-83650101 Msa0478790:CDS 55.0%
AAACCCTTCTGCAGCATGGG+TGG + chr3_3:83650088-83650107 None:intergenic 55.0%
ACCCATGCTGCAGAAGGGTT+TGG - chr3_3:83650087-83650106 Msa0478790:CDS 55.0%
CTCCAAACCCTTCTGCAGCA+TGG + chr3_3:83650092-83650111 None:intergenic 55.0%
! GGGAAGAAAAGGTGGGTTCC+TGG - chr3_3:83650146-83650165 Msa0478790:CDS 55.0%
!! CCTGCTATTTGCTGGCCTTC+TGG + chr3_3:83650353-83650372 None:intergenic 55.0%
CCAGAAGGCCAGCAAATAGC+AGG - chr3_3:83650350-83650369 Msa0478790:intron 55.0%
AAAGGGGTAGGCGGTTCCTT+CGG - chr3_3:83650384-83650403 Msa0478790:intron 55.0%
TACGGCGAGGTTGCAGTATC+CGG + chr3_3:83649970-83649989 None:intergenic 55.0%
TCTCCACCCATGCTGCAGAA+GGG - chr3_3:83650082-83650101 Msa0478790:CDS 55.0%
AAACCCTTCTGCAGCATGGG+TGG + chr3_3:83650088-83650107 None:intergenic 55.0%
ACCCATGCTGCAGAAGGGTT+TGG - chr3_3:83650087-83650106 Msa0478790:CDS 55.0%
CTCCAAACCCTTCTGCAGCA+TGG + chr3_3:83650092-83650111 None:intergenic 55.0%
! GGGAAGAAAAGGTGGGTTCC+TGG - chr3_3:83650146-83650165 Msa0478790:CDS 55.0%
!! CCTGCTATTTGCTGGCCTTC+TGG + chr3_3:83650353-83650372 None:intergenic 55.0%
CCAGAAGGCCAGCAAATAGC+AGG - chr3_3:83650350-83650369 Msa0478790:intron 55.0%
AAAGGGGTAGGCGGTTCCTT+CGG - chr3_3:83650384-83650403 Msa0478790:intron 55.0%
GCCCTTAAAGGGTTACGGCG+AGG + chr3_3:83649983-83650002 None:intergenic 60.0%
CTTCTGCAGCATGGGTGGAG+AGG + chr3_3:83650083-83650102 None:intergenic 60.0%
CTCTCCACCCATGCTGCAGA+AGG - chr3_3:83650081-83650100 Msa0478790:CDS 60.0%
GCCCTTAAAGGGTTACGGCG+AGG + chr3_3:83649983-83650002 None:intergenic 60.0%
CTTCTGCAGCATGGGTGGAG+AGG + chr3_3:83650083-83650102 None:intergenic 60.0%
CTCTCCACCCATGCTGCAGA+AGG - chr3_3:83650081-83650100 Msa0478790:CDS 60.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 83649804 83651579 83649804 ID=Msa0478790;Name=Msa0478790
chr3_3 mRNA 83649804 83651579 83649804 ID=Msa0478790-mRNA-1;Parent=Msa0478790;Name=Msa0478790-mRNA-1;_AED=0.05;_eAED=0.05;_QI=149|1|1|1|1|1|5|252|205
chr3_3 exon 83649804 83650198 83649804 ID=Msa0478790-mRNA-1:exon:23584;Parent=Msa0478790-mRNA-1
chr3_3 exon 83650291 83650351 83650291 ID=Msa0478790-mRNA-1:exon:23583;Parent=Msa0478790-mRNA-1
chr3_3 exon 83650444 83650517 83650444 ID=Msa0478790-mRNA-1:exon:23582;Parent=Msa0478790-mRNA-1
chr3_3 exon 83650994 83651072 83650994 ID=Msa0478790-mRNA-1:exon:23581;Parent=Msa0478790-mRNA-1
chr3_3 exon 83651170 83651579 83651170 ID=Msa0478790-mRNA-1:exon:23580;Parent=Msa0478790-mRNA-1
chr3_3 five_prime_UTR 83651431 83651579 83651431 ID=Msa0478790-mRNA-1:five_prime_utr;Parent=Msa0478790-mRNA-1
chr3_3 CDS 83651170 83651430 83651170 ID=Msa0478790-mRNA-1:cds;Parent=Msa0478790-mRNA-1
chr3_3 CDS 83650994 83651072 83650994 ID=Msa0478790-mRNA-1:cds;Parent=Msa0478790-mRNA-1
chr3_3 CDS 83650444 83650517 83650444 ID=Msa0478790-mRNA-1:cds;Parent=Msa0478790-mRNA-1
chr3_3 CDS 83650291 83650351 83650291 ID=Msa0478790-mRNA-1:cds;Parent=Msa0478790-mRNA-1
chr3_3 CDS 83650056 83650198 83650056 ID=Msa0478790-mRNA-1:cds;Parent=Msa0478790-mRNA-1
chr3_3 three_prime_UTR 83649804 83650055 83649804 ID=Msa0478790-mRNA-1:three_prime_utr;Parent=Msa0478790-mRNA-1
Gene Sequence

>Msa0478790

ATGCAAGGAATCTACCGGATACTGCAACCTCGCCGTAACCCTTTAAGGGCTGCAATATTTCAACAATTCCACGGTAATGGAATGTTTGGCCTGAACAAGTTATCTTCAGGCTTTGGATGTGGCACATCCTCTCCACCCATGCTGCAGAAGGGTTTGGAGAATGTTACAGTATCAGAGGTACTAATGACAAAGGGGGAAGAAAAGGTGGGTTCCTGGCTTTGGTGTAGAGTCGATGATGCTGTTTTTAATGCAATGAAGAATATGGCTGAAAATGATATTGGATCATTGGTGGTACTGAAGCCAGAAGGCCAGCAAATAGCAGGCATTGTCACAGAAAGGGACTGCTTGAAGAAAATAGTTGCAGAAGGAAGATCACCTTTATACACACAAGTTGGACAAATAATGACTAATGAGAATGACCTGGTAACTGTGACCTCTGACACAAACATCCTAAGAGCAATGAGACTAATGTTAGAGAATCGAATCCGTCATGTTCCAGTTATAGATGGGAAAATAGTCGGTATGATTTCAATTGTAGATGTGGTGCGAGCAGTGACAGAGCAACAAAGTGGAGAATTGAAGCGACTCGGTGATTACATCAGAGGAGAATACTATTAG
Protein sequence

>Msa0478790

MQGIYRILQPRRNPLRAAIFQQFHGNGMFGLNKLSSGFGCGTSSPPMLQKGLENVTVSEVLMTKGEEKVGSWLWCRVDDAVFNAMKNMAENDIGSLVVLKPEGQQIAGIVTERDCLKKIVAEGRSPLYTQVGQIMTNENDLVTVTSDTNILRAMRLMLENRIRHVPVIDGKIVGMISIVDVVRAVTEQQSGELKRLGDYIRGEYY*