AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0482730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0482730 MtrunA17_Chr1g0202331 97.191 178 5 0 1 178 364 541 3.41e-118 354
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0482730 AT5G24080.3 58.857 175 66 3 5 178 243 412 1.04e-59 198
Msa0482730 AT5G24080.1 58.857 175 66 3 5 178 243 412 1.04e-59 198
Msa0482730 AT5G24080.2 58.857 175 66 3 5 178 370 539 4.26e-59 198

Find 111 sgRNAs with CRISPR-Local

Find 249 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTGCGTTGCTTCTGTTTA+TGG 0.107198 3_3:+90145733 Msa0482730:CDS
TCCAAAGTTTGGTAAGTTTC+TGG 0.155790 3_3:-90145138 None:intergenic
TATATCTAAAGAAGCAATTT+TGG 0.163496 3_3:+90144810 Msa0482730:five_prime_UTR
CGGCCAAGTTATTGCGGTTT+TGG 0.191109 3_3:+90145224 Msa0482730:five_prime_UTR
CATATGCTAACTATTCTTAT+TGG 0.225057 3_3:+90145175 Msa0482730:five_prime_UTR
CATATCGCGCTTTGCAAATC+AGG 0.225308 3_3:+90146076 Msa0482730:CDS
GATATAGGAGAGTTTCTAAT+TGG 0.226855 3_3:-90144793 None:intergenic
CCACCATGAGATGAAGATTT+TGG 0.246452 3_3:-90145051 None:intergenic
GAAAGTTCATTGGTCTTATC+AGG 0.286923 3_3:+90146036 Msa0482730:CDS
AAAATTGCTTCTTTAGATAT+AGG 0.293497 3_3:-90144808 None:intergenic
GACCTTCCATCTTGTCCTTC+CGG 0.319679 3_3:-90145868 None:intergenic
TGGTAATGGAGTTTGTAAAT+TGG 0.322295 3_3:+90145453 Msa0482730:five_prime_UTR
ACATAAACAACCACCCTATA+TGG 0.335549 3_3:+90144956 Msa0482730:five_prime_UTR
GTTTCAAATCCATGTGATAT+TGG 0.351194 3_3:+90145421 Msa0482730:five_prime_UTR
TCCAGAAACTTACCAAACTT+TGG 0.366550 3_3:+90145137 Msa0482730:five_prime_UTR
TCATTATCATTCTTAGCTTC+CGG 0.373799 3_3:-90145253 None:intergenic
CTCCTCAATACCCAACAAAA+TGG 0.375097 3_3:-90145772 None:intergenic
TATCGTTGGGAAGACGTAAA+TGG 0.375284 3_3:+90145367 Msa0482730:five_prime_UTR
GAAACTGTTAGAGGCTGGTT+TGG 0.376434 3_3:-90145012 None:intergenic
TATGAGAATTCATCTCTTGT+TGG 0.378701 3_3:+90145547 Msa0482730:five_prime_UTR
AATTCTGAAGAAACTGTTAG+AGG 0.383226 3_3:-90145021 None:intergenic
CTACCAACTTTAGAAGTTCC+TGG 0.390678 3_3:-90145511 None:intergenic
ATTAGCATTTGAAGTGTTTG+TGG 0.391442 3_3:-90144887 None:intergenic
TTCCGGTGTCCATGAACTAT+TGG 0.391534 3_3:-90145851 None:intergenic
GTAAGTTAGTGCAAGGCTTA+AGG 0.394651 3_3:-90145109 None:intergenic
GTATTGAGGAGTTTAAGTTT+TGG 0.399082 3_3:+90145784 Msa0482730:CDS
ACAATCATTCCTAAAACAAT+TGG 0.402754 3_3:-90145943 None:intergenic
TCTAGTACTGTTTGTGGCAT+TGG 0.404691 3_3:-90148140 None:intergenic
ATCAAAGTTTAGAATTTCAA+TGG 0.415576 3_3:+90145603 Msa0482730:CDS
AGTCTCCGAAGAACGGTTAA+CGG 0.422756 3_3:-90145313 None:intergenic
GCATCTTGCACTTGTTTGCC+AGG 0.425069 3_3:+90145493 Msa0482730:five_prime_UTR
CTTGTTGGAAAATGTACTAA+TGG 0.430689 3_3:+90145562 Msa0482730:five_prime_UTR
ATTGGAGGAATTTGTGGTAA+TGG 0.436540 3_3:+90145439 Msa0482730:five_prime_UTR
TATAAATTAGAGCATACAAT+TGG 0.436648 3_3:-90148445 None:intergenic
TTCTCCCATTGGTGGTCTCA+TGG 0.438294 3_3:-90148069 None:intergenic
TCAGCAACCATGTCAGAATC+AGG 0.438995 3_3:+90144916 Msa0482730:five_prime_UTR
AATAGTTCTTCTGATGGAAT+GGG 0.440815 3_3:+90145892 Msa0482730:CDS
GAAATTTGAGGTTATATCGT+TGG 0.441793 3_3:+90145353 Msa0482730:five_prime_UTR
TGATAATGATGATGCAGGTT+TGG 0.447690 3_3:+90145267 Msa0482730:five_prime_UTR
AAGAGAAATGGAAAGTTCAT+TGG 0.449679 3_3:+90146026 Msa0482730:CDS
CTGAAGAAACTGTTAGAGGC+TGG 0.453960 3_3:-90145017 None:intergenic
CCTGAGATTTCCAATGTAAC+CGG 0.455323 3_3:+90145204 Msa0482730:five_prime_UTR
TAATAGTTCTTCTGATGGAA+TGG 0.457388 3_3:+90145891 Msa0482730:CDS
GTGGCAGATAGAAGCTTAAA+CGG 0.460444 3_3:+90147978 Msa0482730:exon
ATTAACTCTAGTACTGTTTG+TGG 0.460851 3_3:-90148146 None:intergenic
TGAAGTGTTTGTGGCCCATA+TGG 0.461705 3_3:-90144878 None:intergenic
TGATGGAATGGGGAGGGCTA+AGG 0.464530 3_3:+90145903 Msa0482730:CDS
AGGTTAGAGCCAATAGTTCA+TGG 0.466113 3_3:+90145842 Msa0482730:CDS
CCGGTTACATTGGAAATCTC+AGG 0.467602 3_3:-90145204 None:intergenic
AGTACTGTTTGTGGCATTGG+TGG 0.475028 3_3:-90148137 None:intergenic
AAATTTGAGGTTATATCGTT+GGG 0.476004 3_3:+90145354 Msa0482730:five_prime_UTR
CTAAGCCGTTAACCGTTCTT+CGG 0.476205 3_3:+90145308 Msa0482730:five_prime_UTR
GATCCAAAACCGCAATAACT+TGG 0.476805 3_3:-90145227 None:intergenic
TTTGGAAGGAGTGTGTTAGA+AGG 0.482167 3_3:-90144994 None:intergenic
TTCTGTTTATGGACTAAATG+AGG 0.486123 3_3:+90145744 Msa0482730:CDS
CTGTTAGAGGCTGGTTTGGA+AGG 0.487567 3_3:-90145008 None:intergenic
AGTGCAAGGCTTAAGGAAGT+TGG 0.488094 3_3:-90145102 None:intergenic
GAAACAATGGGTGACACAAT+GGG 0.489853 3_3:+90145393 Msa0482730:five_prime_UTR
TGTGATATTGGAGGAATTTG+TGG 0.491228 3_3:+90145433 Msa0482730:five_prime_UTR
TTATTATAGGAGATGACAAA+TGG 0.494552 3_3:+90147942 Msa0482730:intron
GCGCGATATGTAAAATTCAC+TGG 0.501698 3_3:-90146063 None:intergenic
ATCATCTTGTATGCACCAAA+AGG 0.506963 3_3:-90148042 None:intergenic
ACTTCTAAAGTTGGTAGAGA+TGG 0.511359 3_3:+90145517 Msa0482730:five_prime_UTR
TTGCCAGGAACTTCTAAAGT+TGG 0.519401 3_3:+90145508 Msa0482730:five_prime_UTR
AGAGTTAATTGAGGAAGGGT+TGG 0.522660 3_3:+90148160 Msa0482730:three_prime_UTR
CGAAACAATGGGTGACACAA+TGG 0.524322 3_3:+90145392 Msa0482730:five_prime_UTR
GGTGGTTGTTTATGTTGTGA+AGG 0.526421 3_3:-90144948 None:intergenic
CTCACCACTTCTCCCATTGG+TGG 0.527543 3_3:-90148077 None:intergenic
GTACTAGAGTTAATTGAGGA+AGG 0.529556 3_3:+90148155 Msa0482730:three_prime_UTR
ACGTAAATGGATCGAAACAA+TGG 0.535811 3_3:+90145380 Msa0482730:five_prime_UTR
AATGGGGAGGGCTAAGGAGA+AGG 0.538380 3_3:+90145909 Msa0482730:CDS
AAGAATGATAATGATGATGC+AGG 0.538756 3_3:+90145262 Msa0482730:five_prime_UTR
AATAACTTGGCCGGTTACAT+TGG 0.541303 3_3:-90145214 None:intergenic
GCTAACTATTCTTATTGGCA+AGG 0.543777 3_3:+90145180 Msa0482730:five_prime_UTR
AAGTTCTACTTTGTTTGTGA+AGG 0.549614 3_3:+90145822 Msa0482730:CDS
GAGTGTTAGTCTCCGAAGAA+CGG 0.554635 3_3:-90145320 None:intergenic
AATCTCACCACTTCTCCCAT+TGG 0.555772 3_3:-90148080 None:intergenic
TGTCGATGACTAATCTCCGC+TGG 0.555879 3_3:-90144641 None:intergenic
CGTAAATGGATCGAAACAAT+GGG 0.556068 3_3:+90145381 Msa0482730:five_prime_UTR
CAAAACCGCAATAACTTGGC+CGG 0.556292 3_3:-90145223 None:intergenic
TCAAATCCATGTGATATTGG+AGG 0.560629 3_3:+90145424 Msa0482730:five_prime_UTR
TGTTTCCATGAGACCACCAA+TGG 0.560849 3_3:+90148064 Msa0482730:three_prime_UTR
TCACCAAAATCTTCATCTCA+TGG 0.563558 3_3:+90145048 Msa0482730:five_prime_UTR
TAATGGTGAATGAACTATAG+TGG 0.564330 3_3:-90148217 None:intergenic
GAAGATTGTAAGTTAGTGCA+AGG 0.565458 3_3:-90145116 None:intergenic
TCGAAGAGAACGGAAATTTG+AGG 0.566827 3_3:+90145341 Msa0482730:five_prime_UTR
TTACTATCTTGGTGACATTG+TGG 0.577686 3_3:-90148276 None:intergenic
TACTAGAGTTAATTGAGGAA+GGG 0.579187 3_3:+90148156 Msa0482730:three_prime_UTR
GAGACCACCAATGGGAGAAG+TGG 0.583206 3_3:+90148073 Msa0482730:three_prime_UTR
TTCTTCTGATGGAATGGGGA+GGG 0.583273 3_3:+90145897 Msa0482730:CDS
AACAGTACTAGAGTTAATTG+AGG 0.583430 3_3:+90148151 Msa0482730:three_prime_UTR
GTTTCTTCTTTATCAAAATG+CGG 0.584927 3_3:+90145688 Msa0482730:CDS
AGATGTAAGATGACTGGTAA+TGG 0.587337 3_3:-90148234 None:intergenic
AGGTCTAATAGTTCTTCTGA+TGG 0.587982 3_3:+90145886 Msa0482730:CDS
TGTAACCGGCCAAGTTATTG+CGG 0.588256 3_3:+90145218 Msa0482730:five_prime_UTR
GTTTCCATGAGACCACCAAT+GGG 0.590828 3_3:+90148065 Msa0482730:three_prime_UTR
TGGACACCGGAAGGACAAGA+TGG 0.593660 3_3:+90145862 Msa0482730:CDS
AGTCATCTTTAATAGAGTAG+TGG 0.606982 3_3:-90144607 None:intergenic
GAAGTGGTGAGATTACTTGA+AGG 0.609019 3_3:+90148089 Msa0482730:three_prime_UTR
CAAATTCCTCCAATATCACA+TGG 0.609451 3_3:-90145430 None:intergenic
AGCCAATAGTTCATGGACAC+CGG 0.614174 3_3:+90145849 Msa0482730:CDS
AATAGTTCATGGACACCGGA+AGG 0.620546 3_3:+90145853 Msa0482730:CDS
TGCATGAGATGTAAGATGAC+TGG 0.623779 3_3:-90148240 None:intergenic
AGACTAACACTCGAAGAGAA+CGG 0.628286 3_3:+90145331 Msa0482730:five_prime_UTR
CACCGGAAGGACAAGATGGA+AGG 0.632116 3_3:+90145866 Msa0482730:CDS
GAAGTTGCCTGATTCTGACA+TGG 0.651458 3_3:-90144923 None:intergenic
ATAGTTCTTCTGATGGAATG+GGG 0.654534 3_3:+90145893 Msa0482730:CDS
GTTCTTCTGATGGAATGGGG+AGG 0.659918 3_3:+90145896 Msa0482730:CDS
CCAAAATCTTCATCTCATGG+TGG 0.689250 3_3:+90145051 Msa0482730:five_prime_UTR
AAATGGATCAGCAATCAAAG+TGG 0.692037 3_3:+90147959 Msa0482730:CDS
TGACTAGCAAAATTATCCAG+CGG 0.692319 3_3:+90144625 Msa0482730:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTGAATTTTATAGTAAAG+AGG + chr3_3:90146144-90146163 Msa0482730:intron 15.0%
!! TAACAATTAAACTTAACATA+AGG - chr3_3:90146260-90146279 None:intergenic 15.0%
!!! TTACTTTGGTTTTAAAATAT+GGG + chr3_3:90146398-90146417 Msa0482730:intron 15.0%
!!! CTAACAGTTTATATATTTTT+AGG + chr3_3:90146627-90146646 Msa0482730:intron 15.0%
!! AAAATTGCTTCTTTAGATAT+AGG - chr3_3:90144811-90144830 None:intergenic 20.0%
!!! TATATCTAAAGAAGCAATTT+TGG + chr3_3:90144810-90144829 Msa0482730:five_prime_UTR 20.0%
!!! TTTTTTTGTCTATGAGAATA+AGG - chr3_3:90144855-90144874 None:intergenic 20.0%
!!! ATCAAAGTTTAGAATTTCAA+TGG + chr3_3:90145603-90145622 Msa0482730:CDS 20.0%
!! CATCTTTAAATTGAAATGAT+TGG + chr3_3:90146186-90146205 Msa0482730:intron 20.0%
!!! GTTACTTTGGTTTTAAAATA+TGG + chr3_3:90146397-90146416 Msa0482730:intron 20.0%
!!! TACTTTGGTTTTAAAATATG+GGG + chr3_3:90146399-90146418 Msa0482730:intron 20.0%
!!! ACAGTTTATATATTTTTAGG+TGG + chr3_3:90146630-90146649 Msa0482730:intron 20.0%
!!! TATATATTTTTAGGTGGATT+TGG + chr3_3:90146636-90146655 Msa0482730:intron 20.0%
!! TAAAATCTCAATGGTTTAAT+TGG + chr3_3:90146914-90146933 Msa0482730:intron 20.0%
!!! TCATCTAACAATATATTTTC+TGG - chr3_3:90147144-90147163 None:intergenic 20.0%
!! TTTATAGTATATCAGCATTT+CGG + chr3_3:90147433-90147452 Msa0482730:intron 20.0%
!!! ATTGATGTTCTTTAACTTAA+GGG - chr3_3:90147524-90147543 None:intergenic 20.0%
!!! ACCTAAAACATTTAAAATTG+TGG + chr3_3:90147673-90147692 Msa0482730:intron 20.0%
!!! ACCACAATTTTAAATGTTTT+AGG - chr3_3:90147677-90147696 None:intergenic 20.0%
!! GTTATTAAAATTGTAAGTGT+CGG - chr3_3:90147788-90147807 None:intergenic 20.0%
!!! AAATGAGTTTTATTTTTGGA+TGG - chr3_3:90148325-90148344 None:intergenic 20.0%
!!! GACAAAATGAGTTTTATTTT+TGG - chr3_3:90148329-90148348 None:intergenic 20.0%
!! TATAAATTAGAGCATACAAT+TGG - chr3_3:90148448-90148467 None:intergenic 20.0%
! TAGACAAAAAAATCACCATA+TGG + chr3_3:90144863-90144882 Msa0482730:five_prime_UTR 25.0%
! AGACAAAAAAATCACCATAT+GGG + chr3_3:90144864-90144883 Msa0482730:five_prime_UTR 25.0%
!! ATTCATTTTCATCCAAAGTT+TGG - chr3_3:90145152-90145171 None:intergenic 25.0%
! CATATGCTAACTATTCTTAT+TGG + chr3_3:90145175-90145194 Msa0482730:five_prime_UTR 25.0%
!! AAATTTGAGGTTATATCGTT+GGG + chr3_3:90145354-90145373 Msa0482730:five_prime_UTR 25.0%
!! TTGATGTCATGTTTTCATTT+TGG - chr3_3:90145588-90145607 None:intergenic 25.0%
! GTTTCTTCTTTATCAAAATG+CGG + chr3_3:90145688-90145707 Msa0482730:CDS 25.0%
!! GTGATTATTCCAATTGTTTT+AGG + chr3_3:90145934-90145953 Msa0482730:CDS 25.0%
! ACAATCATTCCTAAAACAAT+TGG - chr3_3:90145946-90145965 None:intergenic 25.0%
!! ACGAACTTTAAAAAGAGAAA+TGG + chr3_3:90146014-90146033 Msa0482730:CDS 25.0%
!! GAATTTTATAGTAAAGAGGA+AGG + chr3_3:90146148-90146167 Msa0482730:intron 25.0%
! TAATTGTTACACAAATGATG+TGG + chr3_3:90146271-90146290 Msa0482730:intron 25.0%
!! AATAAACAATTTTGTCCATG+AGG - chr3_3:90146350-90146369 None:intergenic 25.0%
!!! ATTATTTTAGGATAACGCAT+AGG + chr3_3:90146373-90146392 Msa0482730:intron 25.0%
!! ATAACAGTCAATTTTGTATG+TGG + chr3_3:90146455-90146474 Msa0482730:intron 25.0%
!!! ATGCTTCTTTTAGAAATCAT+TGG + chr3_3:90147324-90147343 Msa0482730:intron 25.0%
!! CAATCTAGTTTCTCTAATTT+AGG + chr3_3:90147483-90147502 Msa0482730:intron 25.0%
!! GATTGATGTTCTTTAACTTA+AGG - chr3_3:90147525-90147544 None:intergenic 25.0%
! TCTCATTAATTGAACAACTT+CGG - chr3_3:90147727-90147746 None:intergenic 25.0%
! ATGTATATGTCTAATGTTCT+AGG - chr3_3:90147883-90147902 None:intergenic 25.0%
! AATACTGATAGCATAATGTT+TGG + chr3_3:90147919-90147938 Msa0482730:intron 25.0%
! GCATAATGTTTGGTTATTAT+AGG + chr3_3:90147929-90147948 Msa0482730:intron 25.0%
! TTATTATAGGAGATGACAAA+TGG + chr3_3:90147942-90147961 Msa0482730:intron 25.0%
!!! ATTCTTAGCTTTTACTATCT+TGG - chr3_3:90148290-90148309 None:intergenic 25.0%
AGTCATCTTTAATAGAGTAG+TGG - chr3_3:90144610-90144629 None:intergenic 30.0%
GATATAGGAGAGTTTCTAAT+TGG - chr3_3:90144796-90144815 None:intergenic 30.0%
! ATTAGCATTTGAAGTGTTTG+TGG - chr3_3:90144890-90144909 None:intergenic 30.0%
! AATTCTGAAGAAACTGTTAG+AGG - chr3_3:90145024-90145043 None:intergenic 30.0%
TCATTATCATTCTTAGCTTC+CGG - chr3_3:90145256-90145275 None:intergenic 30.0%
! AAGAATGATAATGATGATGC+AGG + chr3_3:90145262-90145281 Msa0482730:five_prime_UTR 30.0%
! GAAATTTGAGGTTATATCGT+TGG + chr3_3:90145353-90145372 Msa0482730:five_prime_UTR 30.0%
GTTTCAAATCCATGTGATAT+TGG + chr3_3:90145421-90145440 Msa0482730:five_prime_UTR 30.0%
!! TGGTAATGGAGTTTGTAAAT+TGG + chr3_3:90145453-90145472 Msa0482730:five_prime_UTR 30.0%
TATGAGAATTCATCTCTTGT+TGG + chr3_3:90145547-90145566 Msa0482730:five_prime_UTR 30.0%
CTTGTTGGAAAATGTACTAA+TGG + chr3_3:90145562-90145581 Msa0482730:five_prime_UTR 30.0%
! TTCTGTTTATGGACTAAATG+AGG + chr3_3:90145744-90145763 Msa0482730:CDS 30.0%
!! GTATTGAGGAGTTTAAGTTT+TGG + chr3_3:90145784-90145803 Msa0482730:CDS 30.0%
! AAGTTCTACTTTGTTTGTGA+AGG + chr3_3:90145822-90145841 Msa0482730:CDS 30.0%
TAATAGTTCTTCTGATGGAA+TGG + chr3_3:90145891-90145910 Msa0482730:CDS 30.0%
AATAGTTCTTCTGATGGAAT+GGG + chr3_3:90145892-90145911 Msa0482730:CDS 30.0%
AAGAGAAATGGAAAGTTCAT+TGG + chr3_3:90146026-90146045 Msa0482730:CDS 30.0%
TGTCTAATGACTGAACTTAA+TGG + chr3_3:90146543-90146562 Msa0482730:intron 30.0%
TAATGACTGAACTTAATGGA+GGG + chr3_3:90146547-90146566 Msa0482730:intron 30.0%
ATAAATTCTTTCTCTCCATG+AGG - chr3_3:90146729-90146748 None:intergenic 30.0%
ATCACTGAAGTAAACACAAT+TGG + chr3_3:90146747-90146766 Msa0482730:intron 30.0%
CATAAGACGTAAAATCTCAA+TGG + chr3_3:90146905-90146924 Msa0482730:intron 30.0%
AGAACAGTTCATTGGATAAA+TGG + chr3_3:90146983-90147002 Msa0482730:intron 30.0%
! AGATGAAAACTTTTGTCCTA+AGG + chr3_3:90147158-90147177 Msa0482730:intron 30.0%
!! ACATCTGCTTTTACAGTTAT+AGG - chr3_3:90147291-90147310 None:intergenic 30.0%
!! CTTCTTTTAGAAATCATTGG+TGG + chr3_3:90147327-90147346 Msa0482730:intron 30.0%
!! TTTTAGAAATCATTGGTGGT+AGG + chr3_3:90147331-90147350 Msa0482730:intron 30.0%
!!! CTATAGAGATGTTTTGATTG+AGG - chr3_3:90147556-90147575 None:intergenic 30.0%
! ATATGTAGATTTTCACTGGT+TGG - chr3_3:90147590-90147609 None:intergenic 30.0%
! GTCAATATGTAGATTTTCAC+TGG - chr3_3:90147594-90147613 None:intergenic 30.0%
AATAAAATGCCAGAATGGAA+AGG - chr3_3:90147847-90147866 None:intergenic 30.0%
AGAAGAATAAAATGCCAGAA+TGG - chr3_3:90147852-90147871 None:intergenic 30.0%
! TATATGTCTAATGTTCTAGG+AGG - chr3_3:90147880-90147899 None:intergenic 30.0%
!!! ATCAGTATTTTTCAAGTGGT+TGG - chr3_3:90147908-90147927 None:intergenic 30.0%
!!! TGCTATCAGTATTTTTCAAG+TGG - chr3_3:90147912-90147931 None:intergenic 30.0%
!!! GAGCTTTAAAAATTGCCTTT+TGG + chr3_3:90148027-90148046 Msa0482730:three_prime_UTR 30.0%
ATTAACTCTAGTACTGTTTG+TGG - chr3_3:90148149-90148168 None:intergenic 30.0%
AACAGTACTAGAGTTAATTG+AGG + chr3_3:90148151-90148170 Msa0482730:three_prime_UTR 30.0%
TACTAGAGTTAATTGAGGAA+GGG + chr3_3:90148156-90148175 Msa0482730:three_prime_UTR 30.0%
!!! TTTTCATAGCTTTGTAGACA+TGG - chr3_3:90148187-90148206 None:intergenic 30.0%
TAATGGTGAATGAACTATAG+TGG - chr3_3:90148220-90148239 None:intergenic 30.0%
TGACTAGCAAAATTATCCAG+CGG + chr3_3:90144625-90144644 Msa0482730:five_prime_UTR 35.0%
ACATAAACAACCACCCTATA+TGG + chr3_3:90144956-90144975 Msa0482730:five_prime_UTR 35.0%
GAGAAAAACTCTGCCATATA+GGG - chr3_3:90144972-90144991 None:intergenic 35.0%
TGAGAAAAACTCTGCCATAT+AGG - chr3_3:90144973-90144992 None:intergenic 35.0%
TCACCAAAATCTTCATCTCA+TGG + chr3_3:90145048-90145067 Msa0482730:five_prime_UTR 35.0%
GAAGATTGTAAGTTAGTGCA+AGG - chr3_3:90145119-90145138 None:intergenic 35.0%
TCCAGAAACTTACCAAACTT+TGG + chr3_3:90145137-90145156 Msa0482730:five_prime_UTR 35.0%
TCCAAAGTTTGGTAAGTTTC+TGG - chr3_3:90145141-90145160 None:intergenic 35.0%
GCTAACTATTCTTATTGGCA+AGG + chr3_3:90145180-90145199 Msa0482730:five_prime_UTR 35.0%
! TGATAATGATGATGCAGGTT+TGG + chr3_3:90145267-90145286 Msa0482730:five_prime_UTR 35.0%
ACGTAAATGGATCGAAACAA+TGG + chr3_3:90145380-90145399 Msa0482730:five_prime_UTR 35.0%
CGTAAATGGATCGAAACAAT+GGG + chr3_3:90145381-90145400 Msa0482730:five_prime_UTR 35.0%
TCAAATCCATGTGATATTGG+AGG + chr3_3:90145424-90145443 Msa0482730:five_prime_UTR 35.0%
CAAATTCCTCCAATATCACA+TGG - chr3_3:90145433-90145452 None:intergenic 35.0%
TGTGATATTGGAGGAATTTG+TGG + chr3_3:90145433-90145452 Msa0482730:five_prime_UTR 35.0%
ATTGGAGGAATTTGTGGTAA+TGG + chr3_3:90145439-90145458 Msa0482730:five_prime_UTR 35.0%
!! ACTTCTAAAGTTGGTAGAGA+TGG + chr3_3:90145517-90145536 Msa0482730:five_prime_UTR 35.0%
!! TTGAGGAGTTTAAGTTTTGG+TGG + chr3_3:90145787-90145806 Msa0482730:CDS 35.0%
AGGTCTAATAGTTCTTCTGA+TGG + chr3_3:90145886-90145905 Msa0482730:CDS 35.0%
ATAGTTCTTCTGATGGAATG+GGG + chr3_3:90145893-90145912 Msa0482730:CDS 35.0%
!! GAAAGTTCATTGGTCTTATC+AGG + chr3_3:90146036-90146055 Msa0482730:CDS 35.0%
ATAACGCATAGGAGTTACTT+TGG + chr3_3:90146384-90146403 Msa0482730:intron 35.0%
CTAATGACTGAACTTAATGG+AGG + chr3_3:90146546-90146565 Msa0482730:intron 35.0%
!!! TGTTTTGATTGATGTGTCAC+AGG + chr3_3:90146572-90146591 Msa0482730:intron 35.0%
!!! GTTTTGATTGATGTGTCACA+GGG + chr3_3:90146573-90146592 Msa0482730:intron 35.0%
! GGATTTGGAAGTGTGTATAA+AGG + chr3_3:90146651-90146670 Msa0482730:intron 35.0%
AAATATAGTACACACCTGTG+TGG - chr3_3:90146825-90146844 None:intergenic 35.0%
TGGCTTGAGCAATATTTGTT+TGG + chr3_3:90146934-90146953 Msa0482730:intron 35.0%
TTGAGCAATATTTGTTTGGC+AGG + chr3_3:90146938-90146957 Msa0482730:intron 35.0%
GTTCATGAAGAACAGTTCAT+TGG + chr3_3:90146975-90146994 Msa0482730:intron 35.0%
TATAGCCATAAATACTGCAC+AGG + chr3_3:90147062-90147081 Msa0482730:intron 35.0%
ATAGCCATAAATACTGCACA+GGG + chr3_3:90147063-90147082 Msa0482730:intron 35.0%
TTCATGAGCAATGCAGAAAT+AGG + chr3_3:90147097-90147116 Msa0482730:intron 35.0%
!! TGTCCTAAGGTATCTGATTT+TGG + chr3_3:90147171-90147190 Msa0482730:intron 35.0%
AGTCCAAAATCAGATACCTT+AGG - chr3_3:90147177-90147196 None:intergenic 35.0%
!! TAAGGTATCTGATTTTGGAC+TGG + chr3_3:90147176-90147195 Msa0482730:intron 35.0%
!!! TTTTGGACTGGCAAAATTGA+TGG + chr3_3:90147188-90147207 Msa0482730:intron 35.0%
!! TAGAGGTACTAGAGGTTATT+TGG + chr3_3:90147242-90147261 Msa0482730:intron 35.0%
AAAGCAGATGTTTACAGCTA+TGG + chr3_3:90147300-90147319 Msa0482730:intron 35.0%
TTGCTCGACAATTACAACTA+CGG - chr3_3:90147466-90147485 None:intergenic 35.0%
AAATGGATCAGCAATCAAAG+TGG + chr3_3:90147959-90147978 Msa0482730:CDS 35.0%
ATCATCTTGTATGCACCAAA+AGG - chr3_3:90148045-90148064 None:intergenic 35.0%
GTACTAGAGTTAATTGAGGA+AGG + chr3_3:90148155-90148174 Msa0482730:three_prime_UTR 35.0%
AGATGTAAGATGACTGGTAA+TGG - chr3_3:90148237-90148256 None:intergenic 35.0%
! TTACTATCTTGGTGACATTG+TGG - chr3_3:90148279-90148298 None:intergenic 35.0%
!!! ATTTTGGAGCTTGACACAAC+AGG + chr3_3:90144826-90144845 Msa0482730:five_prime_UTR 40.0%
GGTGGTTGTTTATGTTGTGA+AGG - chr3_3:90144951-90144970 None:intergenic 40.0%
AAAAACTCTGCCATATAGGG+TGG - chr3_3:90144969-90144988 None:intergenic 40.0%
! TTTGGAAGGAGTGTGTTAGA+AGG - chr3_3:90144997-90145016 None:intergenic 40.0%
! CCACCATGAGATGAAGATTT+TGG - chr3_3:90145054-90145073 None:intergenic 40.0%
CCAAAATCTTCATCTCATGG+TGG + chr3_3:90145051-90145070 Msa0482730:five_prime_UTR 40.0%
GTAAGTTAGTGCAAGGCTTA+AGG - chr3_3:90145112-90145131 None:intergenic 40.0%
CCTGAGATTTCCAATGTAAC+CGG + chr3_3:90145204-90145223 Msa0482730:five_prime_UTR 40.0%
AATAACTTGGCCGGTTACAT+TGG - chr3_3:90145217-90145236 None:intergenic 40.0%
GATCCAAAACCGCAATAACT+TGG - chr3_3:90145230-90145249 None:intergenic 40.0%
AGACTAACACTCGAAGAGAA+CGG + chr3_3:90145331-90145350 Msa0482730:five_prime_UTR 40.0%
TCGAAGAGAACGGAAATTTG+AGG + chr3_3:90145341-90145360 Msa0482730:five_prime_UTR 40.0%
TATCGTTGGGAAGACGTAAA+TGG + chr3_3:90145367-90145386 Msa0482730:five_prime_UTR 40.0%
GAAACAATGGGTGACACAAT+GGG + chr3_3:90145393-90145412 Msa0482730:five_prime_UTR 40.0%
!! TTGCCAGGAACTTCTAAAGT+TGG + chr3_3:90145508-90145527 Msa0482730:five_prime_UTR 40.0%
! CTACCAACTTTAGAAGTTCC+TGG - chr3_3:90145514-90145533 None:intergenic 40.0%
GATTGCGTTGCTTCTGTTTA+TGG + chr3_3:90145733-90145752 Msa0482730:CDS 40.0%
! ATGAGGAGAGACCATTTTGT+TGG + chr3_3:90145761-90145780 Msa0482730:CDS 40.0%
! TGAGGAGAGACCATTTTGTT+GGG + chr3_3:90145762-90145781 Msa0482730:CDS 40.0%
!! GACCATTTTGTTGGGTATTG+AGG + chr3_3:90145770-90145789 Msa0482730:CDS 40.0%
CTCCTCAATACCCAACAAAA+TGG - chr3_3:90145775-90145794 None:intergenic 40.0%
AGGTTAGAGCCAATAGTTCA+TGG + chr3_3:90145842-90145861 Msa0482730:CDS 40.0%
GCGCGATATGTAAAATTCAC+TGG - chr3_3:90146066-90146085 None:intergenic 40.0%
! AGTAACTTTTCGCAGCTTCT+AGG + chr3_3:90146102-90146121 Msa0482730:CDS 40.0%
GTAAAGAGGAAGGAGTGAAT+AGG + chr3_3:90146158-90146177 Msa0482730:intron 40.0%
! TGTCTCTGATTTTTACGCAC+TGG + chr3_3:90146216-90146235 Msa0482730:intron 40.0%
TGAAGATGATGTAGTTCACG+TGG + chr3_3:90146303-90146322 Msa0482730:intron 40.0%
! TGAGGATGTGGTAGTGATTA+TGG - chr3_3:90146332-90146351 None:intergenic 40.0%
TAATCACTACCACATCCTCA+TGG + chr3_3:90146332-90146351 Msa0482730:intron 40.0%
! CAATTTTGTCCATGAGGATG+TGG - chr3_3:90146344-90146363 None:intergenic 40.0%
TATGGGGAAGAGTGACATTT+AGG + chr3_3:90146415-90146434 Msa0482730:intron 40.0%
!! ATTTTGATGTGAAGTCCACG+TGG + chr3_3:90146485-90146504 Msa0482730:intron 40.0%
AGTGTGTATAAAGGAAGCCT+AGG + chr3_3:90146660-90146679 Msa0482730:intron 40.0%
TATAAAGGAAGCCTAGGAGA+TGG + chr3_3:90146666-90146685 Msa0482730:intron 40.0%
! CAATTAGAGTACCATCTCCT+AGG - chr3_3:90146680-90146699 None:intergenic 40.0%
! CTTGACAAGATTTTGCCTCA+TGG + chr3_3:90146711-90146730 Msa0482730:intron 40.0%
CGCTATGCATCATATGAACT+TGG + chr3_3:90146770-90146789 Msa0482730:intron 40.0%
! ATGAACTTGGTTCGTCTATG+CGG + chr3_3:90146783-90146802 Msa0482730:intron 40.0%
CAAGCAATATTCGCTTGATC+AGG - chr3_3:90146867-90146886 None:intergenic 40.0%
ATATTTGTTTGGCAGGCTTC+TGG + chr3_3:90146945-90146964 Msa0482730:intron 40.0%
TAGCCATAAATACTGCACAG+GGG + chr3_3:90147064-90147083 Msa0482730:intron 40.0%
AATCCCCTGTGCAGTATTTA+TGG - chr3_3:90147070-90147089 None:intergenic 40.0%
! ACTGGCAAAATTGATGGCAA+GGG + chr3_3:90147194-90147213 Msa0482730:intron 40.0%
TCACTCTCAAGTGGTAACAA+TGG + chr3_3:90147218-90147237 Msa0482730:intron 40.0%
CAAGTGGTAACAATGGTTAG+AGG + chr3_3:90147225-90147244 Msa0482730:intron 40.0%
!! ACAATGGTTAGAGGTACTAG+AGG + chr3_3:90147234-90147253 Msa0482730:intron 40.0%
AGGTTATTTGGCACCTGAAT+GGG + chr3_3:90147254-90147273 Msa0482730:intron 40.0%
GAAGAAGTCTTCTGCATCAA+AGG - chr3_3:90147373-90147392 None:intergenic 40.0%
AAGACTTCTTCTATCCTGGT+TGG + chr3_3:90147382-90147401 Msa0482730:intron 40.0%
AGACTTCTTCTATCCTGGTT+GGG + chr3_3:90147383-90147402 Msa0482730:intron 40.0%
ACAATTACAACTACGGTCCT+AGG - chr3_3:90147459-90147478 None:intergenic 40.0%
AACAACTTCGGTCAACTTAG+TGG - chr3_3:90147715-90147734 None:intergenic 40.0%
TTAACGTCACCTTTCCATTC+TGG + chr3_3:90147835-90147854 Msa0482730:intron 40.0%
GTGGCAGATAGAAGCTTAAA+CGG + chr3_3:90147978-90147997 Msa0482730:exon 40.0%
GAAGTGGTGAGATTACTTGA+AGG + chr3_3:90148089-90148108 Msa0482730:three_prime_UTR 40.0%
TCTAGTACTGTTTGTGGCAT+TGG - chr3_3:90148143-90148162 None:intergenic 40.0%
AGAGTTAATTGAGGAAGGGT+TGG + chr3_3:90148160-90148179 Msa0482730:three_prime_UTR 40.0%
TGCATGAGATGTAAGATGAC+TGG - chr3_3:90148243-90148262 None:intergenic 40.0%
! TGAAGTGTTTGTGGCCCATA+TGG - chr3_3:90144881-90144900 None:intergenic 45.0%
TCAGCAACCATGTCAGAATC+AGG + chr3_3:90144916-90144935 Msa0482730:five_prime_UTR 45.0%
GAAGTTGCCTGATTCTGACA+TGG - chr3_3:90144926-90144945 None:intergenic 45.0%
! GAAACTGTTAGAGGCTGGTT+TGG - chr3_3:90145015-90145034 None:intergenic 45.0%
CTGAAGAAACTGTTAGAGGC+TGG - chr3_3:90145020-90145039 None:intergenic 45.0%
AGTGCAAGGCTTAAGGAAGT+TGG - chr3_3:90145105-90145124 None:intergenic 45.0%
CCGGTTACATTGGAAATCTC+AGG - chr3_3:90145207-90145226 None:intergenic 45.0%
TGTAACCGGCCAAGTTATTG+CGG + chr3_3:90145218-90145237 Msa0482730:five_prime_UTR 45.0%
CAAAACCGCAATAACTTGGC+CGG - chr3_3:90145226-90145245 None:intergenic 45.0%
!!! ATTGCGGTTTTGGATCAAGC+CGG + chr3_3:90145234-90145253 Msa0482730:five_prime_UTR 45.0%
CTAAGCCGTTAACCGTTCTT+CGG + chr3_3:90145308-90145327 Msa0482730:five_prime_UTR 45.0%
AGTCTCCGAAGAACGGTTAA+CGG - chr3_3:90145316-90145335 None:intergenic 45.0%
! GAGTGTTAGTCTCCGAAGAA+CGG - chr3_3:90145323-90145342 None:intergenic 45.0%
CGAAACAATGGGTGACACAA+TGG + chr3_3:90145392-90145411 Msa0482730:five_prime_UTR 45.0%
AGCCAATAGTTCATGGACAC+CGG + chr3_3:90145849-90145868 Msa0482730:CDS 45.0%
TTCCGGTGTCCATGAACTAT+TGG - chr3_3:90145854-90145873 None:intergenic 45.0%
AATAGTTCATGGACACCGGA+AGG + chr3_3:90145853-90145872 Msa0482730:CDS 45.0%
TTCTTCTGATGGAATGGGGA+GGG + chr3_3:90145897-90145916 Msa0482730:CDS 45.0%
CATATCGCGCTTTGCAAATC+AGG + chr3_3:90146076-90146095 Msa0482730:CDS 45.0%
!! TTTTCGCAGCTTCTAGGAAC+AGG + chr3_3:90146108-90146127 Msa0482730:CDS 45.0%
TGAATATGTGACGATCCACG+TGG - chr3_3:90146503-90146522 None:intergenic 45.0%
! TCTATGCGGCTTTTGTTCTG+AGG + chr3_3:90146797-90146816 Msa0482730:intron 45.0%
! CTATGCGGCTTTTGTTCTGA+GGG + chr3_3:90146798-90146817 Msa0482730:intron 45.0%
TGGATCTTCCCTTCATATCG+AGG + chr3_3:90147003-90147022 Msa0482730:intron 45.0%
GGATCTTCCCTTCATATCGA+GGG + chr3_3:90147004-90147023 Msa0482730:intron 45.0%
TATCTCGCCCTCGATATGAA+GGG - chr3_3:90147014-90147033 None:intergenic 45.0%
GGCGAGATAGATTGCTAGAT+TGG + chr3_3:90147025-90147044 Msa0482730:intron 45.0%
! GACTGGCAAAATTGATGGCA+AGG + chr3_3:90147193-90147212 Msa0482730:intron 45.0%
GAGGTTATTTGGCACCTGAA+TGG + chr3_3:90147253-90147272 Msa0482730:intron 45.0%
GGTCGATTACTAACCCATTC+AGG - chr3_3:90147270-90147289 None:intergenic 45.0%
GCAGAAGACTTCTTCTATCC+TGG + chr3_3:90147378-90147397 Msa0482730:intron 45.0%
TATACCTTGTAAGCCCAACC+AGG - chr3_3:90147399-90147418 None:intergenic 45.0%
GTATATCAGCATTTCGGCCT+AGG + chr3_3:90147439-90147458 Msa0482730:intron 45.0%
TGTTTCCATGAGACCACCAA+TGG + chr3_3:90148064-90148083 Msa0482730:three_prime_UTR 45.0%
GTTTCCATGAGACCACCAAT+GGG + chr3_3:90148065-90148084 Msa0482730:three_prime_UTR 45.0%
AATCTCACCACTTCTCCCAT+TGG - chr3_3:90148083-90148102 None:intergenic 45.0%
! AGTACTGTTTGTGGCATTGG+TGG - chr3_3:90148140-90148159 None:intergenic 45.0%
!!! TTGTTTATTATAATTATTTT+AGG + chr3_3:90146361-90146380 Msa0482730:intron 5.0%
!!! CAAAAAAATAAAATTATTTT+AGG - chr3_3:90148351-90148370 None:intergenic 5.0%
TGTCGATGACTAATCTCCGC+TGG - chr3_3:90144644-90144663 None:intergenic 50.0%
!! CTGTTAGAGGCTGGTTTGGA+AGG - chr3_3:90145011-90145030 None:intergenic 50.0%
! CGGCCAAGTTATTGCGGTTT+TGG + chr3_3:90145224-90145243 Msa0482730:five_prime_UTR 50.0%
GCATCTTGCACTTGTTTGCC+AGG + chr3_3:90145493-90145512 Msa0482730:five_prime_UTR 50.0%
GACCTTCCATCTTGTCCTTC+CGG - chr3_3:90145871-90145890 None:intergenic 50.0%
GTTCTTCTGATGGAATGGGG+AGG + chr3_3:90145896-90145915 Msa0482730:CDS 50.0%
CACTGGCAACTTTGCTACTG+TGG + chr3_3:90146233-90146252 Msa0482730:intron 50.0%
GTGTCACAGGGAGAGAAGTT+AGG + chr3_3:90146585-90146604 Msa0482730:intron 50.0%
!! TTTGTTCTGAGGGACCACAC+AGG + chr3_3:90146808-90146827 Msa0482730:intron 50.0%
CTATCTCGCCCTCGATATGA+AGG - chr3_3:90147015-90147034 None:intergenic 50.0%
CTATCCTGGTTGGGCTTACA+AGG + chr3_3:90147392-90147411 Msa0482730:intron 50.0%
! TTCTCCCATTGGTGGTCTCA+TGG - chr3_3:90148072-90148091 None:intergenic 50.0%
TGGACACCGGAAGGACAAGA+TGG + chr3_3:90145862-90145881 Msa0482730:CDS 55.0%
CACCGGAAGGACAAGATGGA+AGG + chr3_3:90145866-90145885 Msa0482730:CDS 55.0%
TGATGGAATGGGGAGGGCTA+AGG + chr3_3:90145903-90145922 Msa0482730:CDS 55.0%
AATGGGGAGGGCTAAGGAGA+AGG + chr3_3:90145909-90145928 Msa0482730:CDS 55.0%
GGCAAGGGATCACTCTCAAG+TGG + chr3_3:90147209-90147228 Msa0482730:intron 55.0%
GAGACCACCAATGGGAGAAG+TGG + chr3_3:90148073-90148092 Msa0482730:three_prime_UTR 55.0%
CTCACCACTTCTCCCATTGG+TGG - chr3_3:90148080-90148099 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 90144600 90148509 90144600 ID=Msa0482730;Name=Msa0482730
chr3_3 mRNA 90144600 90148509 90144600 ID=Msa0482730-mRNA-1;Parent=Msa0482730;Name=Msa0482730-mRNA-1;_AED=0.15;_eAED=0.15;_QI=998|0|1|1|0|1|2|521|189
chr3_3 exon 90144600 90146129 90144600 ID=Msa0482730-mRNA-1:exon:26303;Parent=Msa0482730-mRNA-1
chr3_3 exon 90147951 90148509 90147951 ID=Msa0482730-mRNA-1:exon:26304;Parent=Msa0482730-mRNA-1
chr3_3 five_prime_UTR 90144600 90145597 90144600 ID=Msa0482730-mRNA-1:five_prime_utr;Parent=Msa0482730-mRNA-1
chr3_3 CDS 90145598 90146129 90145598 ID=Msa0482730-mRNA-1:cds;Parent=Msa0482730-mRNA-1
chr3_3 CDS 90147951 90147988 90147951 ID=Msa0482730-mRNA-1:cds;Parent=Msa0482730-mRNA-1
chr3_3 three_prime_UTR 90147989 90148509 90147989 ID=Msa0482730-mRNA-1:three_prime_utr;Parent=Msa0482730-mRNA-1
Gene Sequence

>Msa0482730

ATGACATCAAAGTTTAGAATTTCAATGGTGCAGCAAACTAATTATTATTTTTCTGAGTCTTCAATAATAGCTAATTTTAGTGAGAATGATGTTTCTTCTTTATCAAAATGCGGTGATGCATGTTTATCAGATTGTGATTGCGTTGCTTCTGTTTATGGACTAAATGAGGAGAGACCATTTTGTTGGGTATTGAGGAGTTTAAGTTTTGGTGGTTTTGAAGATACAAGTTCTACTTTGTTTGTGAAGGTTAGAGCCAATAGTTCATGGACACCGGAAGGACAAGATGGAAGGTCTAATAGTTCTTCTGATGGAATGGGGAGGGCTAAGGAGAAGGCTGTGATTATTCCAATTGTTTTAGGAATGATTGTTCTCATTTTTTTACTATGTATGTTACTCTATTACAGTGTTCATAGAAAACGAACTTTAAAAAGAGAAATGGAAAGTTCATTGGTCTTATCAGGTGCTCCAGTGAATTTTACATATCGCGCTTTGCAAATCAGGACGAGTAACTTTTCGCAGCTTCTAGGAACAGGAGATGACAAATGGATCAGCAATCAAAGTGGCAGATAG
Protein sequence

>Msa0482730

MTSKFRISMVQQTNYYFSESSIIANFSENDVSSLSKCGDACLSDCDCVASVYGLNEERPFCWVLRSLSFGGFEDTSSTLFVKVRANSSWTPEGQDGRSNSSSDGMGRAKEKAVIIPIVLGMIVLIFLLCMLLYYSVHRKRTLKREMESSLVLSGAPVNFTYRALQIRTSNFSQLLGTGDDKWISNQSGR*