AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0501050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0501050 MtrunA17_Chr3g0133371 63.830 188 24 3 1 148 105 288 7.67e-75 224
Msa0501050 MtrunA17_Chr3g0133391 66.489 188 22 2 1 148 203 389 1.09e-67 209
Msa0501050 MtrunA17_Chr3g0133481 57.672 189 34 4 1 148 206 389 7.56e-62 194
Msa0501050 MtrunA17_Chr3g0133411 55.319 188 41 3 1 148 254 438 4.78e-60 191
Msa0501050 MtrunA17_Chr3g0083871 56.085 189 38 4 1 147 65 250 5.54e-60 186
Msa0501050 MtrunA17_Chr3g0133431 57.979 188 34 4 1 146 221 405 2.14e-59 189
Msa0501050 MtrunA17_Chr3g0141031 56.085 189 39 3 1 148 35 220 2.12e-58 181
Msa0501050 MtrunA17_Chr3g0083861 55.851 188 39 3 1 147 256 440 1.26e-57 185
Msa0501050 MtrunA17_Chr4g0023671 54.255 188 43 2 1 148 204 388 3.41e-57 182
Msa0501050 MtrunA17_Chr2g0302611 53.403 191 42 3 1 147 200 387 5.96e-57 182
Msa0501050 MtrunA17_Chr2g0302581 53.403 191 42 3 1 147 200 387 1.82e-56 181
Msa0501050 MtrunA17_Chr4g0046911 53.226 186 42 2 1 146 78 258 2.00e-56 177
Msa0501050 MtrunA17_Chr7g0233691 51.852 189 50 2 1 148 90 278 2.68e-56 177
Msa0501050 MtrunA17_Chr2g0302701 52.356 191 44 3 1 147 26 213 4.60e-56 174
Msa0501050 MtrunA17_Chr8g0358841 51.064 188 51 2 1 147 81 268 5.46e-56 176
Msa0501050 MtrunA17_Chr8g0346621 53.723 188 42 3 1 148 215 397 1.03e-55 179
Msa0501050 MtrunA17_Chr7g0233721 51.042 192 47 3 1 148 90 278 2.48e-55 174
Msa0501050 MtrunA17_Chr3g0133511 51.295 193 47 3 1 146 206 398 5.44e-55 177
Msa0501050 MtrunA17_Chr3g0133531 50.000 186 48 3 2 146 123 304 2.72e-50 162
Msa0501050 MtrunA17_Chr7g0233731 49.462 186 51 3 1 144 216 400 1.13e-49 165
Msa0501050 MtrunA17_Chr3g0134681 51.596 188 45 4 1 146 233 416 1.37e-49 164
Msa0501050 MtrunA17_Chr3g0133401 50.532 188 13 3 1 148 1 148 4.80e-49 154
Msa0501050 MtrunA17_Chr7g0233701 50.265 189 53 3 1 148 208 396 8.49e-49 161
Msa0501050 MtrunA17_Chr3g0096411 52.353 170 51 5 1 145 225 389 2.10e-42 144
Msa0501050 MtrunA17_Chr3g0133501 48.408 157 38 2 33 146 1 157 1.35e-41 135
Msa0501050 MtrunA17_Chr3g0133471 47.879 165 43 3 1 122 1 165 1.72e-41 136
Msa0501050 MtrunA17_Chr8g0346651 51.282 156 31 2 33 148 1 151 1.37e-40 133
Msa0501050 MtrunA17_Chr3g0083041 43.750 192 55 5 1 147 115 298 6.37e-39 133
Msa0501050 MtrunA17_Chr3g0133091 51.408 142 27 2 1 100 34 175 4.54e-37 125
Msa0501050 MtrunA17_Chr3g0134651 46.000 150 40 2 1 110 12 160 1.05e-33 116
Msa0501050 MtrunA17_Chr4g0046961 61.458 96 32 2 48 143 28 118 3.88e-30 106
Msa0501050 MtrunA17_Chr3g0143151 36.735 196 71 8 1 148 220 410 4.08e-26 101
Msa0501050 MtrunA17_Chr3g0083001 43.114 167 49 4 24 147 209 372 5.93e-25 98.2
Msa0501050 MtrunA17_Chr3g0132691 80.000 50 10 0 1 50 1 50 5.86e-24 88.6
Msa0501050 MtrunA17_Chr3g0132731 74.000 50 13 0 1 50 87 136 2.66e-21 84.3
Msa0501050 MtrunA17_Chr3g0133491 75.000 36 9 0 113 148 11 46 6.71e-13 59.3
Msa0501050 MtrunA17_Chr4g0046951 68.293 41 13 0 1 41 78 118 7.51e-13 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0501050 AT1G47710.1 46.277 188 58 4 1 146 204 390 2.43e-46 155
Msa0501050 AT1G47710.2 46.277 188 58 4 1 146 231 417 5.99e-46 154
Msa0501050 AT3G45220.1 42.188 192 57 4 1 145 206 390 1.84e-40 139
Msa0501050 AT2G26390.1 44.385 187 56 6 1 145 206 386 2.18e-40 139
Msa0501050 AT2G25240.1 42.781 187 59 6 1 145 202 382 3.10e-38 134
Msa0501050 AT1G64010.1 41.071 168 70 5 1 145 20 181 8.64e-35 120
Msa0501050 AT1G64010.2 41.071 168 70 5 1 145 34 195 1.43e-34 119
Msa0501050 AT1G62170.1 40.120 167 74 6 1 145 269 431 1.77e-27 105
Msa0501050 AT1G62170.2 40.120 167 74 6 1 145 302 464 2.56e-27 105
Msa0501050 AT1G64030.1 38.095 168 80 5 1 145 205 371 3.42e-26 102
Msa0501050 AT2G14540.1 43.373 166 72 5 1 145 233 397 9.60e-24 95.5
Msa0501050 AT1G62160.1 41.463 164 68 7 5 145 60 218 8.83e-21 84.7
Msa0501050 AT2G35580.1 33.918 171 83 5 1 145 205 371 7.15e-20 84.7

Find 76 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTAATGCACTGTACTTTAA+AGG 0.119947 3_4:-41712383 Msa0501050:five_prime_UTR
CTAGAAAAGAAGTGAATTTA+TGG 0.193975 3_4:-41712484 Msa0501050:five_prime_UTR
CCATTCTTTATTAATGTTTC+GGG 0.198311 3_4:+41714090 None:intergenic
TCCATTCTTTATTAATGTTT+CGG 0.205505 3_4:+41714089 None:intergenic
GAAAGATTTGTGGAATATAT+TGG 0.236993 3_4:+41711924 None:intergenic
CTTCTTCTTGCTGGTCATAA+AGG 0.249810 3_4:+41712280 None:intergenic
TCATCTTTGCCTTGCTTATA+CGG 0.269941 3_4:+41712215 None:intergenic
CAATTATGCTAAGCACAATT+TGG 0.273316 3_4:+41713971 None:intergenic
GTTGAGATGATAGGAGGATT+TGG 0.317509 3_4:+41713897 None:intergenic
AATAAACTGCTTCTTCTTGC+TGG 0.320996 3_4:+41712271 None:intergenic
GAAATCAACCGAAGTAATAT+TGG 0.334744 3_4:+41713726 None:intergenic
CACTGTACTTTAAAGGACTA+TGG 0.351189 3_4:-41712376 Msa0501050:five_prime_UTR
TTTCTTCCAAATGCAAAAGA+TGG 0.368008 3_4:-41712170 Msa0501050:CDS
GACAAAGTAGTTGAGATGAT+AGG 0.382402 3_4:+41713888 None:intergenic
TCCGACGCTGCTCCCAATGC+TGG 0.388558 3_4:-41713841 Msa0501050:five_prime_UTR
ACCGGAACAATCATCTTTGT+TGG 0.389749 3_4:-41711760 Msa0501050:CDS
TTCTTCTTGCTGGTCATAAA+GGG 0.398726 3_4:+41712281 None:intergenic
AAAAGATGGGTTGGCAGCTT+TGG 0.400986 3_4:-41712156 Msa0501050:CDS
TTACTTCAATGAAAGATTTG+TGG 0.403242 3_4:+41711914 None:intergenic
ATAATTGCTGCCGGCGCTGA+GGG 0.415624 3_4:-41713955 Msa0501050:five_prime_UTR
TTTGGTTTGTTATTGATTCA+CGG 0.436219 3_4:+41714057 None:intergenic
AAGAGAGTGAAAAGTTCGTT+TGG 0.437971 3_4:+41714039 None:intergenic
CAGGTGCTTAATCCTCTTGA+TGG 0.439086 3_4:-41711736 Msa0501050:CDS
TAGAAAAGAAGTGAATTTAT+GGG 0.440693 3_4:-41712483 Msa0501050:five_prime_UTR
AACAATCATCTTTGTTGGAC+AGG 0.440935 3_4:-41711755 Msa0501050:CDS
GAGAGGCGAGGTCCAGCATT+GGG 0.450306 3_4:+41713829 None:intergenic
GCCACAGTTTGTTTGAAAGA+AGG 0.450408 3_4:+41713763 None:intergenic
GATGATAGGAGGATTTGGAT+TGG 0.450524 3_4:+41713902 None:intergenic
TCCAACAAAGATGATTGTTC+CGG 0.452329 3_4:+41711759 None:intergenic
TTCATTGAAGTAAATGAAGA+AGG 0.457204 3_4:-41711904 Msa0501050:CDS
CCCGAAACATTAATAAAGAA+TGG 0.464552 3_4:-41714090 Msa0501050:five_prime_UTR
TTAAAGAAATTCTTCCTCAA+GGG 0.477536 3_4:-41712436 Msa0501050:five_prime_UTR
GATAGGAGGATTTGGATTGG+AGG 0.478086 3_4:+41713905 None:intergenic
CATAATTGCTGCCGGCGCTG+AGG 0.479644 3_4:-41713956 Msa0501050:five_prime_UTR
GTCAGCCCACCACCAAAGAA+TGG 0.482628 3_4:+41711988 None:intergenic
ATTAAAGAAATTCTTCCTCA+AGG 0.489087 3_4:-41712437 Msa0501050:five_prime_UTR
AGAGAGGCGAGGTCCAGCAT+TGG 0.491358 3_4:+41713828 None:intergenic
TTCTTCCAAATGCAAAAGAT+GGG 0.493850 3_4:-41712169 Msa0501050:CDS
CTGTGTGGGACCCTCAGCGC+CGG 0.495025 3_4:+41713945 None:intergenic
GTTGGCAGCTTTGGTTGAAA+AGG 0.497725 3_4:-41712147 Msa0501050:intron
GTGCTTAGCATAATTGCTGC+CGG 0.499260 3_4:-41713964 Msa0501050:five_prime_UTR
TGCATTAGCAAAGATGAGTC+TGG 0.499282 3_4:+41712397 None:intergenic
TCCAAATGCAAAAGATGGGT+TGG 0.513480 3_4:-41712165 Msa0501050:CDS
TCCAGCATTGGGAGCAGCGT+CGG 0.521967 3_4:+41713840 None:intergenic
CTAATTAGAGATGATTTGAC+CGG 0.538425 3_4:-41711778 Msa0501050:CDS
AGATGAGTCTGGTTAAGTTG+TGG 0.543037 3_4:+41712408 None:intergenic
TTGTTATTGATTCACGGAGA+CGG 0.543116 3_4:+41714063 None:intergenic
TCTCTAATTAGATATAAGAA+TGG 0.543906 3_4:+41711790 None:intergenic
AAAGTAGTTGAGATGATAGG+AGG 0.550259 3_4:+41713891 None:intergenic
TTTCAAAGCCAATATTACTT+CGG 0.555785 3_4:-41713734 Msa0501050:five_prime_UTR
TCTGATATGCTAAAGGAGCT+AGG 0.556026 3_4:-41712021 Msa0501050:intron
GCCACAAAATCCATTCGAGT+TGG 0.558047 3_4:+41711820 None:intergenic
TCGAGAAGTTGTTGCTGTGT+GGG 0.558925 3_4:+41713931 None:intergenic
TATGCGTGGTCCAACTCGAA+TGG 0.563121 3_4:-41711830 Msa0501050:CDS
CATTGGGAGCAGCGTCGGAA+AGG 0.563417 3_4:+41713845 None:intergenic
TCTTAATGGCAGCTCACTCA+AGG 0.565048 3_4:-41712306 Msa0501050:five_prime_UTR
TTCGGTTGATTTCGTGAACA+AGG 0.570400 3_4:-41713716 Msa0501050:intron
TGAGTGAGCTGCCATTAAGA+AGG 0.574459 3_4:+41712309 None:intergenic
AGGTGCTTAATCCTCTTGAT+GGG 0.583872 3_4:-41711735 Msa0501050:CDS
TGCTAAAATCACCCATCAAG+AGG 0.587103 3_4:+41711724 None:intergenic
TAATTAGATATAAGAATGGA+TGG 0.589002 3_4:+41711794 None:intergenic
ACCTTCTTTCAAACAAACTG+TGG 0.593361 3_4:-41713764 Msa0501050:five_prime_UTR
CTTGGTCTTCCGTATAAGCA+AGG 0.596297 3_4:-41712224 Msa0501050:CDS
ATTCGAGTTGGACCACGCAT+AGG 0.605721 3_4:+41711832 None:intergenic
AAGCACAATTTGGAGTGACA+AGG 0.621657 3_4:+41713981 None:intergenic
GTCGAGAAGTTGTTGCTGTG+TGG 0.626344 3_4:+41713930 None:intergenic
AGAAGGCACTGAAGCTGCTG+CGG 0.627362 3_4:-41711887 Msa0501050:CDS
CGTCGGAAAGGACAAAGGAA+AGG 0.637263 3_4:+41713857 None:intergenic
AGCACAATTTGGAGTGACAA+GGG 0.647225 3_4:+41713982 None:intergenic
AGCAGCGTCGGAAAGGACAA+AGG 0.652255 3_4:+41713852 None:intergenic
TTATTGATTCACGGAGACGG+AGG 0.669410 3_4:+41714066 None:intergenic
TATGCTAAAGGAGCTAGGTG+TGG 0.687658 3_4:-41712016 Msa0501050:intron
ACACACCATTGACAAAAGAG+AGG 0.687746 3_4:+41713812 None:intergenic
CTACAATGTGCACCTATGCG+TGG 0.689293 3_4:-41711844 Msa0501050:CDS
AAGTTGTGGATTGACCCTTG+AGG 0.765882 3_4:+41712422 None:intergenic
CCATTGACAAAAGAGAGGCG+AGG 0.775252 3_4:+41713817 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATAATAAATTGGTTAA+TGG + chr3_4:41712295-41712314 None:intergenic 10.0%
!! AAAAGAGTAAAATAATAAAT+TGG + chr3_4:41712302-41712321 None:intergenic 10.0%
!! TGTTTATATGATAAAATATT+AGG - chr3_4:41712694-41712713 Msa0501050:intron 10.0%
!!! ATATACAACTTTAAAAATAT+TGG + chr3_4:41713145-41713164 None:intergenic 10.0%
!! AAAGTTGTATATATTTAATT+TGG - chr3_4:41713153-41713172 Msa0501050:intron 10.0%
!! ATTCAAGAATAATAAAAAGA+GGG + chr3_4:41712248-41712267 None:intergenic 15.0%
!! TATTCAAGAATAATAAAAAG+AGG + chr3_4:41712249-41712268 None:intergenic 15.0%
!! GGTAAAATTTATATCAAAAT+AGG - chr3_4:41712715-41712734 Msa0501050:intron 15.0%
!!! TATATTTGATGTTGTTTTAA+AGG - chr3_4:41713403-41713422 Msa0501050:intron 15.0%
!! TCCATTCTTTATTAATGTTT+CGG + chr3_4:41711833-41711852 None:intergenic 20.0%
!! ATGAAATCTTGAAATACTTA+GGG - chr3_4:41712337-41712356 Msa0501050:five_prime_UTR 20.0%
!!! ATGTTTATTTTGTAACAAGT+TGG + chr3_4:41712657-41712676 None:intergenic 20.0%
!!! ACTTGTTACAAAATAAACAT+AGG - chr3_4:41712657-41712676 Msa0501050:intron 20.0%
!! ATAAGCATGAAACAAATTAT+TGG - chr3_4:41712755-41712774 Msa0501050:intron 20.0%
!! TAAGCATGAAACAAATTATT+GGG - chr3_4:41712756-41712775 Msa0501050:intron 20.0%
!! AGTAATATTCATTATTCTTG+CGG - chr3_4:41712921-41712940 Msa0501050:intron 20.0%
!! AAAATTTACACTGTCATATA+GGG + chr3_4:41713119-41713138 None:intergenic 20.0%
!! AAAAATTTACACTGTCATAT+AGG + chr3_4:41713120-41713139 None:intergenic 20.0%
!! TAGAAAAGAAGTGAATTTAT+GGG - chr3_4:41713436-41713455 Msa0501050:intron 20.0%
!! TAATTAGATATAAGAATGGA+TGG + chr3_4:41714128-41714147 None:intergenic 20.0%
!! TCTCTAATTAGATATAAGAA+TGG + chr3_4:41714132-41714151 None:intergenic 20.0%
! CCATTCTTTATTAATGTTTC+GGG + chr3_4:41711832-41711851 None:intergenic 25.0%
!!! TTTGGTTTGTTATTGATTCA+CGG + chr3_4:41711865-41711884 None:intergenic 25.0%
! TTTCAAAGCCAATATTACTT+CGG - chr3_4:41712185-41712204 Msa0501050:CDS 25.0%
! GATGAAATCTTGAAATACTT+AGG - chr3_4:41712336-41712355 Msa0501050:five_prime_UTR 25.0%
! TTCTTACTAATTTCAACACA+AGG - chr3_4:41712399-41712418 Msa0501050:five_prime_UTR 25.0%
! AAAGAAAACAAATTCATCAC+TGG - chr3_4:41712783-41712802 Msa0501050:intron 25.0%
! TTATTCTTGAGCTATGTAAT+TGG - chr3_4:41712883-41712902 Msa0501050:intron 25.0%
! TATTCTTGAGCTATGTAATT+GGG - chr3_4:41712884-41712903 Msa0501050:intron 25.0%
! AAATTTACACTGTCATATAG+GGG + chr3_4:41713118-41713137 None:intergenic 25.0%
! TCTTATGCTAATTAGATGAA+TGG - chr3_4:41713228-41713247 Msa0501050:intron 25.0%
! ATTAACACCTTACAAAAGAT+CGG + chr3_4:41713276-41713295 None:intergenic 25.0%
! CATATATTCTGTTGCTAAAT+TGG + chr3_4:41713318-41713337 None:intergenic 25.0%
! CTAGAAAAGAAGTGAATTTA+TGG - chr3_4:41713435-41713454 Msa0501050:intron 25.0%
! ATTAAAGAAATTCTTCCTCA+AGG - chr3_4:41713482-41713501 Msa0501050:intron 25.0%
! TTAAAGAAATTCTTCCTCAA+GGG - chr3_4:41713483-41713502 Msa0501050:intron 25.0%
!! AAAAACATAGTCTTTCGTTT+TGG + chr3_4:41713588-41713607 None:intergenic 25.0%
!! TATGTTTTTCACCTTCTTAA+TGG - chr3_4:41713599-41713618 Msa0501050:intron 25.0%
!! CAGTTTATTAAAGCTTTTGA+TGG - chr3_4:41713662-41713681 Msa0501050:intron 25.0%
!!! TTTGATGGTTTTAAAGTTCT+TGG - chr3_4:41713677-41713696 Msa0501050:intron 25.0%
! CCAAATGAAATATTGAACTT+CGG + chr3_4:41713868-41713887 None:intergenic 25.0%
! GAAAGATTTGTGGAATATAT+TGG + chr3_4:41713998-41714017 None:intergenic 25.0%
! TTACTTCAATGAAAGATTTG+TGG + chr3_4:41714008-41714027 None:intergenic 25.0%
! TTCATTGAAGTAAATGAAGA+AGG - chr3_4:41714015-41714034 Msa0501050:five_prime_UTR 25.0%
CCCGAAACATTAATAAAGAA+TGG - chr3_4:41711829-41711848 Msa0501050:CDS 30.0%
CAATTATGCTAAGCACAATT+TGG + chr3_4:41711951-41711970 None:intergenic 30.0%
GAAATCAACCGAAGTAATAT+TGG + chr3_4:41712196-41712215 None:intergenic 30.0%
AAGCAGAGACAAGAATAAAT+CGG + chr3_4:41712365-41712384 None:intergenic 30.0%
TTACTAATTTCAACACAAGG+AGG - chr3_4:41712402-41712421 Msa0501050:five_prime_UTR 30.0%
TGGAAATGAATTGAAAGAGA+TGG - chr3_4:41712803-41712822 Msa0501050:intron 30.0%
ATGAATTGAAAGAGATGGAA+AGG - chr3_4:41712808-41712827 Msa0501050:intron 30.0%
TATTCATTATTCTTGCGGTT+TGG - chr3_4:41712926-41712945 Msa0501050:intron 30.0%
ATTCTGTTGCTAAATTGGTT+TGG + chr3_4:41713313-41713332 None:intergenic 30.0%
TTTCTTCCAAATGCAAAAGA+TGG - chr3_4:41713749-41713768 Msa0501050:five_prime_UTR 30.0%
TTCTTCCAAATGCAAAAGAT+GGG - chr3_4:41713750-41713769 Msa0501050:five_prime_UTR 30.0%
CCGAAGTTCAATATTTCATT+TGG - chr3_4:41713865-41713884 Msa0501050:five_prime_UTR 30.0%
TGAAACTTCTGATATGCTAA+AGG - chr3_4:41713891-41713910 Msa0501050:five_prime_UTR 30.0%
CTAATTAGAGATGATTTGAC+CGG - chr3_4:41714141-41714160 Msa0501050:five_prime_UTR 30.0%
TCTGACACACAAAAAAATGC+AGG + chr3_4:41711774-41711793 None:intergenic 35.0%
!! TTGTTATTGATTCACGGAGA+CGG + chr3_4:41711859-41711878 None:intergenic 35.0%
AAGAGAGTGAAAAGTTCGTT+TGG + chr3_4:41711883-41711902 None:intergenic 35.0%
AAAGTAGTTGAGATGATAGG+AGG + chr3_4:41712031-41712050 None:intergenic 35.0%
GACAAAGTAGTTGAGATGAT+AGG + chr3_4:41712034-41712053 None:intergenic 35.0%
ACCTTCTTTCAAACAAACTG+TGG - chr3_4:41712155-41712174 Msa0501050:CDS 35.0%
TAATTTCAACACAAGGAGGA+AGG - chr3_4:41712406-41712425 Msa0501050:five_prime_UTR 35.0%
AATTTCAACACAAGGAGGAA+GGG - chr3_4:41712407-41712426 Msa0501050:five_prime_UTR 35.0%
! AGATAAAAAAGCATGCGGTT+TGG - chr3_4:41712507-41712526 Msa0501050:intron 35.0%
GGAATTATAATCGAACCTGA+TGG - chr3_4:41712829-41712848 Msa0501050:intron 35.0%
!! ATTATTCTTGCGGTTTGGTT+TGG - chr3_4:41712931-41712950 Msa0501050:intron 35.0%
!!! TTGGTTTGCGGTTTTTCTAT+TGG - chr3_4:41713073-41713092 Msa0501050:intron 35.0%
!! TGGTTTGCGGTTTTTCTATT+GGG - chr3_4:41713074-41713093 Msa0501050:intron 35.0%
!!! GTTTTTCTATTGGGTTGGTT+CGG - chr3_4:41713083-41713102 Msa0501050:intron 35.0%
GCTAATGCACTGTACTTTAA+AGG - chr3_4:41713536-41713555 Msa0501050:intron 35.0%
! CACTGTACTTTAAAGGACTA+TGG - chr3_4:41713543-41713562 Msa0501050:intron 35.0%
TTCTTCTTGCTGGTCATAAA+GGG + chr3_4:41713641-41713660 None:intergenic 35.0%
AATAAACTGCTTCTTCTTGC+TGG + chr3_4:41713651-41713670 None:intergenic 35.0%
TCATCTTTGCCTTGCTTATA+CGG + chr3_4:41713707-41713726 None:intergenic 35.0%
!! TTTTACCATTCTTTGGTGGT+GGG - chr3_4:41713926-41713945 Msa0501050:five_prime_UTR 35.0%
TCCAACAAAGATGATTGTTC+CGG + chr3_4:41714163-41714182 None:intergenic 35.0%
AACAATCATCTTTGTTGGAC+AGG - chr3_4:41714164-41714183 Msa0501050:five_prime_UTR 35.0%
ACACACAAAAAAATGCAGGC+AGG + chr3_4:41711770-41711789 None:intergenic 40.0%
! AGCACAATTTGGAGTGACAA+GGG + chr3_4:41711940-41711959 None:intergenic 40.0%
! AAGCACAATTTGGAGTGACA+AGG + chr3_4:41711941-41711960 None:intergenic 40.0%
GATGATAGGAGGATTTGGAT+TGG + chr3_4:41712020-41712039 None:intergenic 40.0%
GTTGAGATGATAGGAGGATT+TGG + chr3_4:41712025-41712044 None:intergenic 40.0%
ACACACCATTGACAAAAGAG+AGG + chr3_4:41712110-41712129 None:intergenic 40.0%
! TCTCTTTTGTCAATGGTGTG+TGG - chr3_4:41712109-41712128 Msa0501050:intron 40.0%
! CTCTTTTGTCAATGGTGTGT+GGG - chr3_4:41712110-41712129 Msa0501050:intron 40.0%
! GCCACAGTTTGTTTGAAAGA+AGG + chr3_4:41712159-41712178 None:intergenic 40.0%
! TTCGGTTGATTTCGTGAACA+AGG - chr3_4:41712203-41712222 Msa0501050:CDS 40.0%
ATTTCAACACAAGGAGGAAG+GGG - chr3_4:41712408-41712427 Msa0501050:five_prime_UTR 40.0%
GCACATATGGTATGACAGTA+GGG - chr3_4:41712430-41712449 Msa0501050:five_prime_UTR 40.0%
CACATATGGTATGACAGTAG+GGG - chr3_4:41712431-41712450 Msa0501050:five_prime_UTR 40.0%
!! TTTTTATCTCGCGGTTTGGA+TGG + chr3_4:41712496-41712515 None:intergenic 40.0%
!!! TGCTTTTTTATCTCGCGGTT+TGG + chr3_4:41712500-41712519 None:intergenic 40.0%
!! AAAAAGCATGCGGTTTGGTT+TGG - chr3_4:41712512-41712531 Msa0501050:intron 40.0%
!! TGGTTTGGTTTGGTTTGGTT+TGG + chr3_4:41712946-41712965 None:intergenic 40.0%
CAAACCAAACCAAACCAATG+CGG - chr3_4:41712565-41712584 Msa0501050:intron 40.0%
!! CAATTCAAACCGCATTGGTT+TGG + chr3_4:41712577-41712596 None:intergenic 40.0%
CAAACCAATGCGGTTTGAAT+TGG - chr3_4:41712575-41712594 Msa0501050:intron 40.0%
CAATGCGGTTTGAATTGGTT+CGG - chr3_4:41712580-41712599 Msa0501050:intron 40.0%
!! TTGAATTGGTTCGGTTTGTG+CGG - chr3_4:41712589-41712608 Msa0501050:intron 40.0%
!! GTTTGGATTGGTTTGGTTTG+CGG - chr3_4:41713061-41713080 Msa0501050:intron 40.0%
! TTGCGGTTTTTCTATTGGGT+TGG - chr3_4:41713078-41713097 Msa0501050:intron 40.0%
! AGTCTCACCGATCTTTTGTA+AGG - chr3_4:41713266-41713285 Msa0501050:intron 40.0%
TGGGCTGAAAAAGAGACAAA+TGG - chr3_4:41713455-41713474 Msa0501050:intron 40.0%
! AGATGAGTCTGGTTAAGTTG+TGG + chr3_4:41713514-41713533 None:intergenic 40.0%
TGCATTAGCAAAGATGAGTC+TGG + chr3_4:41713525-41713544 None:intergenic 40.0%
CTTCTTCTTGCTGGTCATAA+AGG + chr3_4:41713642-41713661 None:intergenic 40.0%
TCCAAATGCAAAAGATGGGT+TGG - chr3_4:41713754-41713773 Msa0501050:five_prime_UTR 40.0%
CAAGCTTCCTCTTGATAAAG+TGG - chr3_4:41713822-41713841 Msa0501050:five_prime_UTR 40.0%
CCTACTTCCACTTTATCAAG+AGG + chr3_4:41713832-41713851 None:intergenic 40.0%
CCTCTTGATAAAGTGGAAGT+AGG - chr3_4:41713829-41713848 Msa0501050:five_prime_UTR 40.0%
AAGTGGAAGTAGGTGACTTT+AGG - chr3_4:41713839-41713858 Msa0501050:five_prime_UTR 40.0%
TCTGATATGCTAAAGGAGCT+AGG - chr3_4:41713898-41713917 Msa0501050:five_prime_UTR 40.0%
!! GGTGTGGTTTTACCATTCTT+TGG - chr3_4:41713919-41713938 Msa0501050:five_prime_UTR 40.0%
!! GTGGTTTTACCATTCTTTGG+TGG - chr3_4:41713922-41713941 Msa0501050:five_prime_UTR 40.0%
! GTTTTACCATTCTTTGGTGG+TGG - chr3_4:41713925-41713944 Msa0501050:five_prime_UTR 40.0%
!! TCCAACTCGAATGGATTTTG+TGG - chr3_4:41714098-41714117 Msa0501050:five_prime_UTR 40.0%
ACCGGAACAATCATCTTTGT+TGG - chr3_4:41714159-41714178 Msa0501050:five_prime_UTR 40.0%
AGGTGCTTAATCCTCTTGAT+GGG - chr3_4:41714184-41714203 Msa0501050:five_prime_UTR 40.0%
!! TTATTGATTCACGGAGACGG+AGG + chr3_4:41711856-41711875 None:intergenic 45.0%
GTGCTTAGCATAATTGCTGC+CGG - chr3_4:41711955-41711974 Msa0501050:CDS 45.0%
TCGAGAAGTTGTTGCTGTGT+GGG + chr3_4:41711991-41712010 None:intergenic 45.0%
! GATAGGAGGATTTGGATTGG+AGG + chr3_4:41712017-41712036 None:intergenic 45.0%
GGCACATATGGTATGACAGT+AGG - chr3_4:41712429-41712448 Msa0501050:five_prime_UTR 45.0%
!!! CCGCATGCTTTTTTATCTCG+CGG + chr3_4:41712505-41712524 None:intergenic 45.0%
! CCGCGAGATAAAAAAGCATG+CGG - chr3_4:41712502-41712521 Msa0501050:intron 45.0%
!! CGCATTGGTTTGGTTTGGTT+TGG + chr3_4:41712567-41712586 None:intergenic 45.0%
!! CAAACCGCATTGGTTTGGTT+TGG + chr3_4:41712572-41712591 None:intergenic 45.0%
CGAACCAATTCAAACCGCAT+TGG + chr3_4:41712582-41712601 None:intergenic 45.0%
! TTTTCCATCGTTGCACCATC+AGG + chr3_4:41712847-41712866 None:intergenic 45.0%
!! TCTTGCGGTTTGGTTTGGTT+TGG - chr3_4:41712936-41712955 Msa0501050:intron 45.0%
!! CGGTTTGGTTTGGTTTGGTT+TGG - chr3_4:41712941-41712960 Msa0501050:intron 45.0%
!! GCACTTGGTTCGGATGAATT+CGG + chr3_4:41713024-41713043 None:intergenic 45.0%
GAATTCATCCGAACCAAGTG+CGG - chr3_4:41713023-41713042 Msa0501050:intron 45.0%
GCAAAAAACCGCACTTGGTT+CGG + chr3_4:41713034-41713053 None:intergenic 45.0%
!!! GAACCAAGTGCGGTTTTTTG+CGG - chr3_4:41713033-41713052 Msa0501050:intron 45.0%
AAACCGCAAAAAACCGCACT+TGG + chr3_4:41713039-41713058 None:intergenic 45.0%
!!! AGTGCGGTTTTTTGCGGTTT+CGG - chr3_4:41713039-41713058 Msa0501050:intron 45.0%
!!! GGTTTTTTGCGGTTTCGGTT+TGG - chr3_4:41713044-41713063 Msa0501050:intron 45.0%
!! TTTGCGGTTTCGGTTTGGAT+TGG - chr3_4:41713049-41713068 Msa0501050:intron 45.0%
!! GGTTTCGGTTTGGATTGGTT+TGG - chr3_4:41713054-41713073 Msa0501050:intron 45.0%
! AAGTTGTGGATTGACCCTTG+AGG + chr3_4:41713500-41713519 None:intergenic 45.0%
TGAGTGAGCTGCCATTAAGA+AGG + chr3_4:41713613-41713632 None:intergenic 45.0%
TCTTAATGGCAGCTCACTCA+AGG - chr3_4:41713613-41713632 Msa0501050:intron 45.0%
! CTTGGTCTTCCGTATAAGCA+AGG - chr3_4:41713695-41713714 Msa0501050:intron 45.0%
! GCCAACCCATCTTTTGCATT+TGG + chr3_4:41713758-41713777 None:intergenic 45.0%
!! AAAAGATGGGTTGGCAGCTT+TGG - chr3_4:41713763-41713782 Msa0501050:five_prime_UTR 45.0%
! GTTGGCAGCTTTGGTTGAAA+AGG - chr3_4:41713772-41713791 Msa0501050:five_prime_UTR 45.0%
TATGCTAAAGGAGCTAGGTG+TGG - chr3_4:41713903-41713922 Msa0501050:five_prime_UTR 45.0%
GCCACAAAATCCATTCGAGT+TGG + chr3_4:41714102-41714121 None:intergenic 45.0%
CAGGTGCTTAATCCTCTTGA+TGG - chr3_4:41714183-41714202 Msa0501050:five_prime_UTR 45.0%
GTCGAGAAGTTGTTGCTGTG+TGG + chr3_4:41711992-41712011 None:intergenic 50.0%
CGTCGGAAAGGACAAAGGAA+AGG + chr3_4:41712065-41712084 None:intergenic 50.0%
CCATTGACAAAAGAGAGGCG+AGG + chr3_4:41712105-41712124 None:intergenic 50.0%
! CCTCGCCTCTCTTTTGTCAA+TGG - chr3_4:41712102-41712121 Msa0501050:intron 50.0%
!! TATGACAGTAGGGGTGTTCG+CGG - chr3_4:41712440-41712459 Msa0501050:five_prime_UTR 50.0%
!! GCATGCGGTTTGGTTTGGTT+CGG - chr3_4:41712517-41712536 Msa0501050:intron 50.0%
! GGCAGCTTTGGTTGAAAAGG+TGG - chr3_4:41713775-41713794 Msa0501050:five_prime_UTR 50.0%
!! TGGTGGTGGGCTGACAAAAA+TGG - chr3_4:41713939-41713958 Msa0501050:five_prime_UTR 50.0%
!! TGGTGGGCTGACAAAAATGG+TGG - chr3_4:41713942-41713961 Msa0501050:five_prime_UTR 50.0%
CTACAATGTGCACCTATGCG+TGG - chr3_4:41714075-41714094 Msa0501050:five_prime_UTR 50.0%
!! ATTCGAGTTGGACCACGCAT+AGG + chr3_4:41714090-41714109 None:intergenic 50.0%
TATGCGTGGTCCAACTCGAA+TGG - chr3_4:41714089-41714108 Msa0501050:five_prime_UTR 50.0%
! ATAATTGCTGCCGGCGCTGA+GGG - chr3_4:41711964-41711983 Msa0501050:CDS 55.0%
! AGCAGCGTCGGAAAGGACAA+AGG + chr3_4:41712070-41712089 None:intergenic 55.0%
! CAAGGAGGAAGGGGCACATA+TGG - chr3_4:41712417-41712436 Msa0501050:five_prime_UTR 55.0%
! CGAACCTGATGGTGCAACGA+TGG - chr3_4:41712840-41712859 Msa0501050:intron 55.0%
! GACTTTTGCACTGAACCGCG+CGG + chr3_4:41712999-41713018 None:intergenic 55.0%
GTCAGCCCACCACCAAAGAA+TGG + chr3_4:41713934-41713953 None:intergenic 55.0%
!! AGAAGGCACTGAAGCTGCTG+CGG - chr3_4:41714032-41714051 Msa0501050:five_prime_UTR 55.0%
CATAATTGCTGCCGGCGCTG+AGG - chr3_4:41711963-41711982 Msa0501050:CDS 60.0%
! CATTGGGAGCAGCGTCGGAA+AGG + chr3_4:41712077-41712096 None:intergenic 60.0%
! TCCAGCATTGGGAGCAGCGT+CGG + chr3_4:41712082-41712101 None:intergenic 60.0%
GAGAGGCGAGGTCCAGCATT+GGG + chr3_4:41712093-41712112 None:intergenic 60.0%
AGAGAGGCGAGGTCCAGCAT+TGG + chr3_4:41712094-41712113 None:intergenic 60.0%
!! GTTCGCGGTGCGGTTTGGTT+CGG - chr3_4:41712455-41712474 Msa0501050:five_prime_UTR 60.0%
!! GCGCGGTTCGGTTTGGTTTG+CGG + chr3_4:41712982-41713001 None:intergenic 60.0%
GCAAACCAAACCGAACCGCG+CGG - chr3_4:41712981-41713000 Msa0501050:intron 60.0%
TTGCACTGAACCGCGCGGTT+CGG + chr3_4:41712994-41713013 None:intergenic 60.0%
TCCGACGCTGCTCCCAATGC+TGG - chr3_4:41712078-41712097 Msa0501050:intron 65.0%
!! CAGTAGGGGTGTTCGCGGTG+CGG - chr3_4:41712445-41712464 Msa0501050:five_prime_UTR 65.0%
!! CTGAACCGCGCGGTTCGGTT+TGG + chr3_4:41712989-41713008 None:intergenic 65.0%
CTGTGTGGGACCCTCAGCGC+CGG + chr3_4:41711977-41711996 None:intergenic 70.0%
! GGGGTGTTCGCGGTGCGGTT+TGG - chr3_4:41712450-41712469 Msa0501050:five_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 41711732 41714209 41711732 ID=Msa0501050;Name=Msa0501050
chr3_4 mRNA 41711732 41714209 41711732 ID=Msa0501050-mRNA-1;Parent=Msa0501050;Name=Msa0501050-mRNA-1;_AED=0.38;_eAED=0.38;_QI=712|0.5|0.33|1|1|1|3|0|148
chr3_4 exon 41711732 41712028 41711732 ID=Msa0501050-mRNA-1:exon:8804;Parent=Msa0501050-mRNA-1
chr3_4 exon 41712148 41712516 41712148 ID=Msa0501050-mRNA-1:exon:8803;Parent=Msa0501050-mRNA-1
chr3_4 exon 41713717 41714209 41713717 ID=Msa0501050-mRNA-1:exon:8802;Parent=Msa0501050-mRNA-1
chr3_4 five_prime_UTR 41713717 41714209 41713717 ID=Msa0501050-mRNA-1:five_prime_utr;Parent=Msa0501050-mRNA-1
chr3_4 five_prime_UTR 41712298 41712516 41712298 ID=Msa0501050-mRNA-1:five_prime_utr;Parent=Msa0501050-mRNA-1
chr3_4 CDS 41712148 41712297 41712148 ID=Msa0501050-mRNA-1:cds;Parent=Msa0501050-mRNA-1
chr3_4 CDS 41711732 41712028 41711732 ID=Msa0501050-mRNA-1:cds;Parent=Msa0501050-mRNA-1
Gene Sequence

>Msa0501050

ATGACCAGCAAGAAGAAGCAGTTTATTAAAGCTTTTGATGGTTTTAAAGTTCTTGGTCTTCCGTATAAGCAAGGCAAAGATGAGCGTCAATTCACTATGTACTTTTTTCTTCCAAATGCAAAAGATGGGTTGGCAGCTTTGGTTGAAAAGGAGCTAGGTGTGGTTTTACCATTCTTTGGTGGTGGGCTGACAAAAATGGTGGACTCTTCTGTGAGTCACGAACTTTTTGTTTCCAATATATTCCACAAATCTTTCATTGAAGTAAATGAAGAAGGCACTGAAGCTGCTGCGGTAAGTGCTGTAACTATAGAACTACAATGTGCACCTATGCGTGGTCCAACTCGAATGGATTTTGTGGCTGACCATCCATTCTTATATCTAATTAGAGATGATTTGACCGGAACAATCATCTTTGTTGGACAGGTGCTTAATCCTCTTGATGGGTGA
Protein sequence

>Msa0501050

MTSKKKQFIKAFDGFKVLGLPYKQGKDERQFTMYFFLPNAKDGLAALVEKELGVVLPFFGGGLTKMVDSSVSHELFVSNIFHKSFIEVNEEGTEAAAVSAVTIELQCAPMRGPTRMDFVADHPFLYLIRDDLTGTIIFVGQVLNPLDG*