AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0504230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0504230 MtrunA17_Chr3g0104061 98.551 552 8 0 6 557 5 556 0.0 1125
Msa0504230 MtrunA17_Chr5g0429511 83.094 556 92 2 4 557 3 558 0.0 982
Msa0504230 MtrunA17_Chr4g0005921 74.410 551 138 2 10 557 7 557 0.0 843
Msa0504230 MtrunA17_Chr5g0436461 65.273 550 189 2 9 557 6 554 0.0 796
Msa0504230 MtrunA17_Chr5g0404341 62.613 551 204 2 9 557 16 566 0.0 736
Msa0504230 MtrunA17_Chr5g0404361 61.497 561 212 2 1 557 51 611 0.0 735
Msa0504230 MtrunA17_Chr7g0238981 62.364 550 191 6 24 557 44 593 0.0 695
Msa0504230 MtrunA17_Chr7g0237491 57.194 563 224 5 12 557 18 580 0.0 668
Msa0504230 MtrunA17_Chr7g0237441 56.483 563 228 6 12 557 18 580 0.0 661
Msa0504230 MtrunA17_Chr7g0238351 56.790 567 227 6 9 557 15 581 0.0 659
Msa0504230 MtrunA17_Chr7g0238941 55.712 569 234 7 6 557 11 578 0.0 643
Msa0504230 MtrunA17_Chr4g0031901 54.307 534 231 5 24 545 41 573 0.0 606
Msa0504230 MtrunA17_Chr5g0437681 48.161 571 275 7 4 557 1 567 0.0 563
Msa0504230 MtrunA17_Chr3g0114211 48.571 560 270 8 10 557 17 570 0.0 560
Msa0504230 MtrunA17_Chr5g0431581 49.462 558 267 6 10 557 8 560 0.0 549
Msa0504230 MtrunA17_Chr8g0346581 48.346 544 262 5 27 557 34 571 0.0 544
Msa0504230 MtrunA17_Chr2g0278811 44.833 600 288 7 1 557 8 607 0.0 528
Msa0504230 MtrunA17_Chr7g0240971 46.491 570 284 6 8 557 2 570 3.07e-173 502
Msa0504230 MtrunA17_Chr4g0008521 44.708 548 287 4 26 557 25 572 2.42e-172 499
Msa0504230 MtrunA17_Chr7g0240991 43.162 563 300 10 13 557 11 571 2.13e-165 482
Msa0504230 MtrunA17_Chr5g0436431 60.965 228 88 1 289 515 1 228 6.00e-99 299
Msa0504230 MtrunA17_Chr3g0133861 42.900 331 176 8 229 547 1 330 1.45e-78 251
Msa0504230 MtrunA17_Chr8g0382691 32.537 544 313 16 23 535 30 550 1.89e-76 252
Msa0504230 MtrunA17_Chr3g0133871 49.763 211 103 1 1 208 3 213 6.23e-69 221
Msa0504230 MtrunA17_Chr3g0118321 32.212 565 332 16 4 536 1 546 1.42e-67 228
Msa0504230 MtrunA17_Chr3g0118331 32.176 547 325 16 17 535 16 544 1.93e-65 223
Msa0504230 MtrunA17_Chr3g0143671 28.269 566 360 15 1 557 5 533 5.90e-55 194
Msa0504230 MtrunA17_Chr1g0157341 29.278 526 328 15 9 523 6 498 3.62e-53 189
Msa0504230 MtrunA17_Chr4g0038671 26.978 556 368 13 8 557 9 532 4.61e-51 183
Msa0504230 MtrunA17_Chr4g0056511 28.345 568 360 21 9 557 10 549 7.58e-48 176
Msa0504230 MtrunA17_Chr4g0024861 27.407 540 347 14 27 557 27 530 1.76e-47 173
Msa0504230 MtrunA17_Chr2g0322441 28.545 543 337 15 27 557 30 533 5.99e-46 169
Msa0504230 MtrunA17_Chr8g0374261 26.786 560 354 17 9 555 9 525 3.28e-45 167
Msa0504230 MtrunA17_Chr3g0079321 27.565 497 314 15 45 522 47 516 4.45e-44 165
Msa0504230 MtrunA17_Chr8g0374271 26.946 501 318 12 27 517 31 493 4.26e-42 159
Msa0504230 MtrunA17_Chr5g0402831 25.607 535 345 16 27 549 31 524 6.05e-40 152
Msa0504230 MtrunA17_Chr4g0043261 26.071 537 357 17 10 537 6 511 1.50e-39 151
Msa0504230 MtrunA17_Chr7g0234061 25.981 535 361 15 28 549 30 542 3.67e-39 151
Msa0504230 MtrunA17_Chr3g0121171 26.015 542 359 16 10 539 1157 1668 1.01e-37 149
Msa0504230 MtrunA17_Chr3g0121171 25.919 544 357 17 10 539 582 1093 1.80e-36 145
Msa0504230 MtrunA17_Chr3g0121171 25.385 520 352 13 28 539 27 518 5.44e-36 144
Msa0504230 MtrunA17_Chr3g0121171 25.641 468 306 15 83 539 1798 2234 4.13e-30 126
Msa0504230 MtrunA17_Chr3g0121161 25.919 544 357 17 10 539 47 558 2.46e-37 145
Msa0504230 MtrunA17_Chr3g0121151 25.646 542 361 17 10 539 47 558 1.34e-36 144
Msa0504230 MtrunA17_Chr4g0075071 26.245 522 344 14 3 517 7 494 2.85e-35 139
Msa0504230 MtrunA17_Chr4g0043251 25.561 579 365 21 8 556 3 545 8.18e-34 135
Msa0504230 MtrunA17_Chr3g0118351 32.923 325 179 10 239 536 5 317 8.16e-33 128
Msa0504230 MtrunA17_Chr4g0024641 27.333 450 275 19 81 517 3 413 2.17e-28 120
Msa0504230 MtrunA17_Chr5g0436411 62.500 72 27 0 11 82 8 79 7.14e-26 101
Msa0504230 MtrunA17_Chr1g0197301 27.635 351 215 11 219 557 6 329 7.87e-25 105
Msa0504230 MtrunA17_Chr3g0118341 39.850 133 76 4 17 146 16 147 7.55e-23 97.1
Msa0504230 MtrunA17_Chr3g0118931 44.595 74 39 1 467 540 10 81 5.73e-13 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0504230 AT2G38080.1 76.044 551 129 2 9 557 9 558 0.0 886
Msa0504230 AT5G01190.1 72.401 558 151 2 3 557 1 558 0.0 830
Msa0504230 AT5G58910.2 71.123 561 151 4 4 557 11 567 0.0 821
Msa0504230 AT5G58910.3 70.945 561 151 5 4 557 11 566 0.0 815
Msa0504230 AT5G01190.2 70.789 558 146 3 3 557 1 544 0.0 801
Msa0504230 AT5G58910.1 71.698 530 136 4 35 557 1 523 0.0 776
Msa0504230 AT5G03260.1 62.545 550 205 1 9 557 8 557 0.0 726
Msa0504230 AT5G60020.1 56.076 576 229 8 6 557 2 577 0.0 667
Msa0504230 AT5G60020.3 57.615 545 209 7 35 557 1 545 0.0 650
Msa0504230 AT2G29130.1 56.106 565 229 9 12 557 9 573 0.0 650
Msa0504230 AT5G60020.2 55.056 534 216 8 6 515 2 535 0.0 597
Msa0504230 AT5G05390.1 49.561 569 271 6 1 557 1 565 0.0 579
Msa0504230 AT2G30210.1 48.432 574 272 8 4 557 1 570 0.0 563
Msa0504230 AT2G40370.1 47.774 584 274 9 1 557 1 580 0.0 558
Msa0504230 AT1G18140.1 48.668 563 263 8 19 557 21 581 0.0 556
Msa0504230 AT2G46570.1 46.585 571 284 7 2 557 5 569 0.0 541
Msa0504230 AT5G07130.1 47.993 573 275 9 4 557 1 569 0.0 533
Msa0504230 AT5G09360.1 45.567 564 293 8 3 557 11 569 0.0 525
Msa0504230 AT3G09220.1 47.436 546 274 6 8 540 5 550 2.60e-173 502
Msa0504230 AT5G01050.1 44.779 565 286 8 9 557 14 568 9.74e-169 491
Msa0504230 AT5G01040.1 44.583 563 288 10 9 557 14 566 5.40e-165 482
Msa0504230 AT2G46570.2 46.407 487 242 5 84 557 4 484 2.45e-161 469
Msa0504230 AT5G07130.2 49.795 488 222 9 89 557 1 484 4.42e-159 463
Msa0504230 AT5G07130.3 49.795 488 222 9 89 557 1 484 4.42e-159 463
Msa0504230 AT5G48100.1 42.385 545 295 11 18 547 13 553 8.95e-152 447
Msa0504230 AT3G09220.2 48.673 452 221 5 100 540 2 453 2.27e-145 427
Msa0504230 AT4G39830.1 31.122 588 350 17 4 557 11 577 4.47e-73 244
Msa0504230 AT5G21105.1 31.453 585 320 18 11 547 22 573 1.07e-66 227
Msa0504230 AT5G21100.1 31.752 548 319 17 21 536 18 542 6.71e-66 224
Msa0504230 AT5G21105.3 30.853 551 301 19 11 515 22 538 8.79e-57 199
Msa0504230 AT4G39830.2 30.480 479 286 13 88 535 3 465 1.58e-52 186
Msa0504230 AT1G21850.1 28.269 520 334 12 9 523 11 496 2.54e-52 187
Msa0504230 AT4G39830.3 30.462 476 284 13 91 535 4 463 7.81e-52 184
Msa0504230 AT1G76160.1 26.617 541 362 13 1 537 1 510 4.41e-50 181
Msa0504230 AT1G41830.1 26.750 557 364 13 9 557 11 531 1.79e-49 179
Msa0504230 AT4G39830.4 29.707 478 278 12 88 535 3 452 1.79e-49 178
Msa0504230 AT4G25240.2 27.951 576 370 18 1 557 1 550 4.32e-49 179
Msa0504230 AT4G25240.1 27.951 576 370 18 1 557 1 550 4.32e-49 179
Msa0504230 AT5G51480.1 27.788 547 351 18 29 557 30 550 4.79e-49 179
Msa0504230 AT4G12420.1 27.205 533 347 16 7 522 4 512 4.30e-48 176
Msa0504230 AT4G12420.2 27.205 533 347 16 7 522 4 512 4.30e-48 176
Msa0504230 AT1G21860.1 27.183 504 324 14 27 523 29 496 7.36e-48 175
Msa0504230 AT1G75790.1 27.925 530 321 16 8 517 7 495 1.01e-47 174
Msa0504230 AT4G22010.1 26.895 554 367 14 10 557 9 530 1.11e-46 172
Msa0504230 AT4G28090.1 27.533 523 329 16 48 557 49 534 2.54e-45 168
Msa0504230 AT5G66920.1 26.493 536 355 13 27 557 37 538 3.47e-45 167
Msa0504230 AT4G38420.1 26.679 521 345 14 5 517 8 499 4.75e-44 164
Msa0504230 AT5G48450.2 27.579 504 334 15 20 517 23 501 1.18e-42 161
Msa0504230 AT5G48450.1 27.579 504 334 15 20 517 23 501 2.22e-42 161
Msa0504230 AT1G55570.1 27.947 526 331 18 10 517 5 500 5.98e-41 156
Msa0504230 AT4G37160.1 25.693 541 351 12 29 557 33 534 8.53e-41 155
Msa0504230 AT2G23630.1 26.937 542 342 14 29 557 32 532 2.07e-39 151
Msa0504230 AT2G23630.2 26.937 542 342 14 29 557 37 537 2.30e-39 151
Msa0504230 AT4G38420.3 26.243 503 334 14 5 499 8 481 2.66e-39 150
Msa0504230 AT1G55560.1 27.000 500 319 16 31 517 30 496 3.77e-37 145
Msa0504230 AT3G13390.1 27.689 502 320 17 28 517 29 499 4.83e-37 145
Msa0504230 AT5G21105.2 31.978 369 198 13 202 547 44 382 8.45e-37 141
Msa0504230 AT4G38420.2 25.895 475 316 13 5 471 8 454 5.35e-35 137
Msa0504230 AT3G13400.2 25.341 513 355 13 9 517 9 497 7.48e-35 138
Msa0504230 AT3G13400.1 25.341 513 355 13 9 517 9 497 7.48e-35 138
Msa0504230 AT4G37160.2 26.804 388 253 8 29 408 33 397 2.70e-31 127

Find 157 sgRNAs with CRISPR-Local

Find 243 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCACATTTCTTCAAGATTAA+AGG 0.156883 3_4:+48422376 Msa0504230:CDS
CCTGTGGTTTATAACTTTAC+AGG 0.192956 3_4:+48422430 Msa0504230:CDS
AAACCAATTGTAACAGTAAA+TGG 0.209691 3_4:+48420747 Msa0504230:CDS
GATGTCCAATTGACATTTAT+TGG 0.212243 3_4:+48423370 Msa0504230:three_prime_UTR
TTGTACAATTTCACTCTTAC+AGG 0.222618 3_4:+48421058 Msa0504230:CDS
AGTTGCAGCTTCTCCTTTCA+TGG 0.245576 3_4:+48422039 Msa0504230:CDS
ATATGACACTGAGTGTTAAT+TGG 0.251646 3_4:-48423271 None:intergenic
AGGAAACTTTGATTCTAAAA+AGG 0.263573 3_4:+48422615 Msa0504230:CDS
ATGCTCATGTCCTTTGGCTT+AGG 0.274973 3_4:+48421104 Msa0504230:CDS
ATAAATGAAGCTTTGAAATC+TGG 0.276343 3_4:+48421379 Msa0504230:CDS
GTTCATTACATTGAAATATA+TGG 0.278764 3_4:-48423302 None:intergenic
AATCATCCTATTCATCTTCA+TGG 0.287524 3_4:+48422562 Msa0504230:CDS
GTGTGAGCATCAGAGGTATT+TGG 0.288436 3_4:-48421409 None:intergenic
GTTGGACTGCTATCAGATTC+AGG 0.291470 3_4:+48422701 Msa0504230:CDS
AAGTTATGATCAATCTTTAA+AGG 0.302248 3_4:-48422238 None:intergenic
ATGGAACAAGTTATTGTATT+AGG 0.303449 3_4:+48421196 Msa0504230:CDS
GTAACAGCCAAACATAAAAT+TGG 0.304548 3_4:+48422007 Msa0504230:CDS
GAGGTGTAGAACTGAAAATA+AGG 0.308125 3_4:+48419825 Msa0504230:five_prime_UTR
AACTTTAGCAGGGAATTTCT+TGG 0.318189 3_4:-48422215 None:intergenic
AACTTGTTCCATATGTGGTT+TGG 0.319821 3_4:-48421185 None:intergenic
ACTTGTTCCATATGTGGTTT+GGG 0.323893 3_4:-48421184 None:intergenic
TCTGTTAACTTGTTATATAT+TGG 0.335274 3_4:+48423545 Msa0504230:three_prime_UTR
GGCCCGGTTGAATCGGGCAT+TGG 0.337655 3_4:-48421027 None:intergenic
TGCTCATGTCCTTTGGCTTA+GGG 0.342579 3_4:+48421105 Msa0504230:CDS
GGTGGCATGCTCATGTCCTT+TGG 0.354997 3_4:+48421098 Msa0504230:CDS
AACCCAATGCCCGATTCAAC+CGG 0.363157 3_4:+48421024 Msa0504230:CDS
CTCCAATAAAATCTTGTCAT+TGG 0.365098 3_4:-48423333 None:intergenic
ATTTACTGTTACAATTGGTT+TGG 0.365814 3_4:-48420745 None:intergenic
TCCATATGTGGTTTGGGAAA+AGG 0.371654 3_4:-48421178 None:intergenic
TAGGTCTGGCCCGGTTGAAT+CGG 0.371672 3_4:-48421034 None:intergenic
GGGTGTCATGGCGGTGGTTG+CGG 0.377626 3_4:+48419847 Msa0504230:exon
CCTCAACAACAGTTAGTTGA+TGG 0.377830 3_4:-48421910 None:intergenic
TAGAACATTGTCATCTTCTC+TGG 0.383332 3_4:-48420796 None:intergenic
CCATATGTGGTTTGGGAAAA+GGG 0.384456 3_4:-48421177 None:intergenic
TAAACCACAGGAGGATTTCC+AGG 0.385148 3_4:-48422418 None:intergenic
TTAAATCCATGAAGATGAAT+AGG 0.388456 3_4:-48422568 None:intergenic
TTTCCATTTACTGTTACAAT+TGG 0.390709 3_4:-48420750 None:intergenic
TCCAATAAAATCTTGTCATT+GGG 0.392277 3_4:-48423332 None:intergenic
TGGCTTAGGGCCACTGTCCA+TGG 0.392475 3_4:+48421118 Msa0504230:CDS
AAGAGTGAAATTGTACAAAT+AGG 0.392591 3_4:-48421053 None:intergenic
TAGCACTTGGGGAGGTCACT+TGG 0.394034 3_4:-48423239 None:intergenic
ACAAATTTAGCAACTACAAA+AGG 0.395229 3_4:+48422463 Msa0504230:CDS
TGTAGGAGGCAAAGAAGTTA+AGG 0.395469 3_4:-48422140 None:intergenic
TATAGCAGAATTTCTTTGTT+GGG 0.397946 3_4:+48419798 Msa0504230:five_prime_UTR
ATCTTTAAAGGAACTTTAGC+AGG 0.398500 3_4:-48422226 None:intergenic
GGCTGTTACAAGCACATTTG+TGG 0.398982 3_4:-48421993 None:intergenic
GTTTCGAATTATACTATTGC+TGG 0.401191 3_4:+48419871 Msa0504230:CDS
AGATGACAATGTTCTAATAA+AGG 0.403469 3_4:+48420803 Msa0504230:CDS
TTATAATTCAACAGTTCAAT+TGG 0.407301 3_4:+48422507 Msa0504230:CDS
TCCTTTGTCTGTGGATGCTA+TGG 0.411257 3_4:+48419907 Msa0504230:CDS
CACCTTGTGAAGCACAGCTT+TGG 0.412151 3_4:-48421456 None:intergenic
AGGTGTAGAACTGAAAATAA+GGG 0.415505 3_4:+48419826 Msa0504230:five_prime_UTR
CACACAATCAATGGTCATCC+AGG 0.417766 3_4:+48421427 Msa0504230:CDS
TTACAAGCACATTTGTGGTT+TGG 0.419033 3_4:-48421988 None:intergenic
GCAACTGAAGTAGCATTTGT+AGG 0.419668 3_4:-48422157 None:intergenic
GTGGTTTATGCATTGCCATT+TGG 0.421349 3_4:+48423136 Msa0504230:CDS
ACTGCCACTTTACACTATTC+AGG 0.423483 3_4:+48422094 Msa0504230:CDS
TCTTCTCTGGCATAGATTGT+AGG 0.425371 3_4:-48420783 None:intergenic
GCCCGGTTGAATCGGGCATT+GGG 0.426724 3_4:-48421026 None:intergenic
ATAGACCAATAAATGTCAAT+TGG 0.429020 3_4:-48423375 None:intergenic
CTATAGCAGAATTTCTTTGT+TGG 0.443466 3_4:+48419797 Msa0504230:five_prime_UTR
ACAATAACTTGTTCCATATG+TGG 0.444307 3_4:-48421190 None:intergenic
GGATTTAATTTCTTTGTAGT+TGG 0.449492 3_4:+48422583 Msa0504230:CDS
CTACGAACGGGTTGGGCCGA+TGG 0.462286 3_4:+48420989 Msa0504230:CDS
AGGTCTGGCCCGGTTGAATC+GGG 0.463494 3_4:-48421033 None:intergenic
GGTGGTAATAGTGATTCATT+TGG 0.467355 3_4:-48423215 None:intergenic
TGAAGCTTTGAAATCTGGAT+TGG 0.468280 3_4:+48421384 Msa0504230:CDS
CACTTGGGGAGGTCACTTGG+AGG 0.469326 3_4:-48423236 None:intergenic
TTGGTACATTTCTTAGCACT+TGG 0.469731 3_4:-48423252 None:intergenic
AGCACATTTGTGGTTTGGCC+AGG 0.472275 3_4:-48421983 None:intergenic
CAAACAACTACGAACGGGTT+GGG 0.473450 3_4:+48420982 Msa0504230:CDS
GGGTAAGGAACTCCAAGCTT+AGG 0.483870 3_4:-48421157 None:intergenic
CTGGCATAGATTGTAGGACC+TGG 0.484477 3_4:-48420777 None:intergenic
TAATTTCTTTGTAGTTGGTA+GGG 0.489391 3_4:+48422588 Msa0504230:CDS
CTGTGGTTTATAACTTTACA+GGG 0.490930 3_4:+48422431 Msa0504230:CDS
TTAATTTCTTTGTAGTTGGT+AGG 0.491457 3_4:+48422587 Msa0504230:CDS
TACGAACGGGTTGGGCCGAT+GGG 0.494187 3_4:+48420990 Msa0504230:CDS
TGAAATTGTACAAATAGGTC+TGG 0.495644 3_4:-48421048 None:intergenic
TACAACATGGGGACTGAAGA+TGG 0.496316 3_4:+48423166 Msa0504230:CDS
AAGCTTAGGTAAAATGACTA+AGG 0.497449 3_4:-48421143 None:intergenic
TTACACTATTCAGGCACACT+TGG 0.499103 3_4:+48422103 Msa0504230:CDS
GGGCATTGGGTTATGTATGC+TGG 0.502957 3_4:-48421013 None:intergenic
AATTTCTTTGTAGTTGGTAG+GGG 0.505618 3_4:+48422589 Msa0504230:CDS
TTGGACTGCTATCAGATTCA+GGG 0.506751 3_4:+48422702 Msa0504230:CDS
GGGGTCAAACAACTACGAAC+GGG 0.508123 3_4:+48420977 Msa0504230:CDS
GCACAGCTTTGGATAGGCCC+TGG 0.510644 3_4:-48421445 None:intergenic
ATAGCAGTCCAACCTCCAGC+TGG 0.511743 3_4:-48422691 None:intergenic
ATGTTGTATGAATTTCCAAA+TGG 0.519335 3_4:-48423151 None:intergenic
AATTTCTTTGTTGGGTAGAG+AGG 0.520522 3_4:+48419806 Msa0504230:five_prime_UTR
TTTGTAGTTGGTAGGGGACA+AGG 0.522356 3_4:+48422595 Msa0504230:CDS
TCTGATGCTCACACAATCAA+TGG 0.523012 3_4:+48421418 Msa0504230:CDS
CAGGTCAAAGAGGCACACTT+TGG 0.524524 3_4:+48421077 Msa0504230:CDS
TCAAACAACTACGAACGGGT+TGG 0.530689 3_4:+48420981 Msa0504230:CDS
TGGTACATTTCTTAGCACTT+GGG 0.534652 3_4:-48423251 None:intergenic
AGATGAATAGGATGATTCTC+AGG 0.535265 3_4:-48422556 None:intergenic
TTTGTTGTGGACAATGGTAA+AGG 0.536832 3_4:+48423191 Msa0504230:CDS
AGATTCAGGGCTGATAATCC+AGG 0.546615 3_4:+48422715 Msa0504230:CDS
CAAATTTAGCAACTACAAAA+GGG 0.550075 3_4:+48422464 Msa0504230:CDS
TTGGGGAGGTCACTTGGAGG+TGG 0.550876 3_4:-48423233 None:intergenic
GCATGCAGGAGGATTCTCAT+TGG 0.551595 3_4:+48421808 Msa0504230:intron
TGTGGTTTGGGAAAAGGGTA+AGG 0.551687 3_4:-48421172 None:intergenic
TAGGTGTTCCAGCTGGAGGT+TGG 0.552597 3_4:+48422683 Msa0504230:CDS
ATGTTTGCTTCCTTTGTCTG+TGG 0.553888 3_4:+48419898 Msa0504230:CDS
ATTGATTGTGTGAGCATCAG+AGG 0.555850 3_4:-48421416 None:intergenic
TTCACTCTTACAGGTCAAAG+AGG 0.557117 3_4:+48421067 Msa0504230:CDS
TGTGAAGCACAGCTTTGGAT+AGG 0.557785 3_4:-48421451 None:intergenic
CTTTCCTGGAAATCCTCCTG+TGG 0.559487 3_4:+48422414 Msa0504230:CDS
ATTTGGAAATTCATACAACA+TGG 0.560438 3_4:+48423153 Msa0504230:CDS
TTGTACAAATAGGTCTGGCC+CGG 0.560533 3_4:-48421043 None:intergenic
GTCAAAGAGGCACACTTTGG+TGG 0.562180 3_4:+48421080 Msa0504230:CDS
GTTCAATCTTGTTGATCCTG+TGG 0.564919 3_4:+48422648 Msa0504230:CDS
CTTCTGATGCAACAAAGACA+AGG 0.565271 3_4:-48423521 None:intergenic
TGGGGTCAAACAACTACGAA+CGG 0.565814 3_4:+48420976 Msa0504230:CDS
ACTGAAGATGGCATTTGTTG+TGG 0.572897 3_4:+48423178 Msa0504230:CDS
CCATCAACTAACTGTTGTTG+AGG 0.573284 3_4:+48421910 Msa0504230:CDS
AGAAGTTAAGGTTGTGAGTG+AGG 0.575305 3_4:-48422128 None:intergenic
GGTTATGTATGCTGGCCCAT+CGG 0.576036 3_4:-48421005 None:intergenic
TTTGGAAATTCATACAACAT+GGG 0.577320 3_4:+48423154 Msa0504230:CDS
AATACAGTAGGTGTTCCAGC+TGG 0.579561 3_4:+48422676 Msa0504230:CDS
GGTACATTTCTTAGCACTTG+GGG 0.580989 3_4:-48423250 None:intergenic
CCTGTAAAGTTATAAACCAC+AGG 0.582741 3_4:-48422430 None:intergenic
AATAAGGGGTGTCATGGCGG+TGG 0.583095 3_4:+48419841 Msa0504230:exon
ACCCAATGCCCGATTCAACC+GGG 0.584434 3_4:+48421025 Msa0504230:CDS
ACAGTAAATGGAAAGTTCCC+AGG 0.584699 3_4:+48420759 Msa0504230:CDS
ACAATAGGTGCATCCATGAA+AGG 0.586114 3_4:-48422052 None:intergenic
TCTTTAAAGGAACTTTAGCA+GGG 0.591893 3_4:-48422225 None:intergenic
ATCCAAAGCTGTGCTTCACA+AGG 0.593137 3_4:+48421454 Msa0504230:CDS
GACACAATTGTTATAGCACC+TGG 0.602253 3_4:+48421965 Msa0504230:CDS
TTGGAAATTCATACAACATG+GGG 0.603938 3_4:+48423155 Msa0504230:CDS
ACTGAAAATAAGGGGTGTCA+TGG 0.605546 3_4:+48419835 Msa0504230:exon
ATGGCATTTGTTGTGGACAA+TGG 0.610790 3_4:+48423185 Msa0504230:CDS
TGAATAGTGTAAAGTGGCAG+TGG 0.612605 3_4:-48422092 None:intergenic
CCTACTGTATTCCTCTCCAC+AGG 0.617875 3_4:-48422664 None:intergenic
ACATTTCTTAGCACTTGGGG+AGG 0.620338 3_4:-48423247 None:intergenic
ACCATAGCATCCACAGACAA+AGG 0.621036 3_4:-48419908 None:intergenic
AATACAATGTTAGCATACAC+TGG 0.622203 3_4:+48420841 Msa0504230:CDS
GGTGTAGAACTGAAAATAAG+GGG 0.624832 3_4:+48419827 Msa0504230:five_prime_UTR
GGACAGTGGCCCTAAGCCAA+AGG 0.625616 3_4:-48421114 None:intergenic
ATCTTGTTGATCCTGTGGAG+AGG 0.625881 3_4:+48422653 Msa0504230:CDS
TGGCATAGATTGTAGGACCT+GGG 0.629411 3_4:-48420776 None:intergenic
ACAGTAGGTGTTCCAGCTGG+AGG 0.633035 3_4:+48422679 Msa0504230:CDS
TGCTAACATTGTATTTGACA+TGG 0.638789 3_4:-48420833 None:intergenic
TTCTGATGCAACAAAGACAA+GGG 0.642194 3_4:-48423520 None:intergenic
TGTGCCTGAATAGTGTAAAG+TGG 0.642791 3_4:-48422098 None:intergenic
ACACAATCAATGGTCATCCA+GGG 0.647979 3_4:+48421428 Msa0504230:CDS
GAAATGTGCTTGAAGAAGCG+CGG 0.650792 3_4:-48422362 None:intergenic
TTTGGTTGAACACAATCTTG+TGG 0.654765 3_4:-48420727 None:intergenic
ACTGAAGTAGCATTTGTAGG+AGG 0.659695 3_4:-48422154 None:intergenic
CCTGTGGAGAGGAATACAGT+AGG 0.664601 3_4:+48422664 Msa0504230:CDS
TAGCAACTACAAAAGGGACA+AGG 0.668259 3_4:+48422470 Msa0504230:CDS
GAAAATAAGGGGTGTCATGG+CGG 0.669808 3_4:+48419838 Msa0504230:exon
TTGTTCACACAAGTAGCACA+AGG 0.670421 3_4:-48422289 None:intergenic
GACTAAGGCACCATGGACAG+TGG 0.677009 3_4:-48421128 None:intergenic
GTAAAGTTATAAACCACAGG+AGG 0.682757 3_4:-48422427 None:intergenic
GTAAAATGACTAAGGCACCA+TGG 0.685444 3_4:-48421135 None:intergenic
TCTGATGCAACAAAGACAAG+GGG 0.688765 3_4:-48423519 None:intergenic
ATGGTGGAAATCAGATACTG+AGG 0.694169 3_4:+48421351 Msa0504230:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CATTAATTTTTTTTATGTAA+AGG + chr3_4:48420689-48420708 Msa0504230:intron 10.0%
!!! TTTAAAATAATGAAGCATAA+AGG + chr3_4:48420094-48420113 Msa0504230:intron 15.0%
!!! AATGAATTGAAATTTTAAAC+AGG + chr3_4:48420951-48420970 Msa0504230:intron 15.0%
!!! TATGGTATTATATTTTGTTA+GGG + chr3_4:48423111-48423130 Msa0504230:intron 15.0%
!!! TTCTTCTTGAATAAAATTTT+AGG + chr3_4:48423447-48423466 Msa0504230:three_prime_UTR 15.0%
!!! TCTTCTTGAATAAAATTTTA+GGG + chr3_4:48423448-48423467 Msa0504230:three_prime_UTR 15.0%
!!! ACTTCTTTAAATTTTGAATG+TGG + chr3_4:48420164-48420183 Msa0504230:intron 20.0%
!! GTAAATGTTACTACTAAATT+TGG + chr3_4:48420191-48420210 Msa0504230:intron 20.0%
!!! TTCAATCCAAAGATTTTATT+AGG + chr3_4:48420470-48420489 Msa0504230:intron 20.0%
!! TCATAACCTAATAAAATCTT+TGG - chr3_4:48420479-48420498 None:intergenic 20.0%
!! ATATGACTAAATGAACAAAT+TGG - chr3_4:48420526-48420545 None:intergenic 20.0%
!!! TGGTCAAGTATTTTTTTAAT+AGG - chr3_4:48420559-48420578 None:intergenic 20.0%
!!! TTTTTTTTTGTTTAGGTGAA+TGG + chr3_4:48421332-48421351 Msa0504230:intron 20.0%
!!! GAAATTTCCAATTTTATGTT+TGG - chr3_4:48422017-48422036 None:intergenic 20.0%
!! AAGTTATGATCAATCTTTAA+AGG - chr3_4:48422241-48422260 None:intergenic 20.0%
!! TTATAATTCAACAGTTCAAT+TGG + chr3_4:48422507-48422526 Msa0504230:CDS 20.0%
!! ATAATCCAGGTAAATTTATT+AGG + chr3_4:48422728-48422747 Msa0504230:intron 20.0%
!!! ACTAATGATTTGTATCAATT+GGG - chr3_4:48422780-48422799 None:intergenic 20.0%
!!! CGATTCATTATGATAAATTT+TGG + chr3_4:48422837-48422856 Msa0504230:intron 20.0%
!!! CATAATTTCATTTTTCATCA+TGG - chr3_4:48423015-48423034 None:intergenic 20.0%
!!! GTATGGTATTATATTTTGTT+AGG + chr3_4:48423110-48423129 Msa0504230:intron 20.0%
!!! ATGGTATTATATTTTGTTAG+GGG + chr3_4:48423112-48423131 Msa0504230:intron 20.0%
!! GTTCATTACATTGAAATATA+TGG - chr3_4:48423305-48423324 None:intergenic 20.0%
!!! TCTGTTAACTTGTTATATAT+TGG + chr3_4:48423545-48423564 Msa0504230:three_prime_UTR 20.0%
! TATAGCAGAATTTCTTTGTT+GGG + chr3_4:48419798-48419817 Msa0504230:five_prime_UTR 25.0%
!! ACTTTTTGTTAAGTTTGACT+AGG - chr3_4:48420234-48420253 None:intergenic 25.0%
! TTCAATTCAAATGATATTGC+AGG + chr3_4:48420285-48420304 Msa0504230:intron 25.0%
! TCATTTAGTCATATATGTCT+TGG + chr3_4:48420532-48420551 Msa0504230:intron 25.0%
! ATTTACTGTTACAATTGGTT+TGG - chr3_4:48420748-48420767 None:intergenic 25.0%
! AAACCAATTGTAACAGTAAA+TGG + chr3_4:48420747-48420766 Msa0504230:CDS 25.0%
! TTTCCATTTACTGTTACAAT+TGG - chr3_4:48420753-48420772 None:intergenic 25.0%
! AGATGACAATGTTCTAATAA+AGG + chr3_4:48420803-48420822 Msa0504230:CDS 25.0%
!! ATTGAAATTTTAAACAGGCA+TGG + chr3_4:48420956-48420975 Msa0504230:intron 25.0%
!! TTGAAATTTTAAACAGGCAT+GGG + chr3_4:48420957-48420976 Msa0504230:intron 25.0%
! AAGAGTGAAATTGTACAAAT+AGG - chr3_4:48421056-48421075 None:intergenic 25.0%
! ATGGAACAAGTTATTGTATT+AGG + chr3_4:48421196-48421215 Msa0504230:CDS 25.0%
!!! ATAAATGAAGCTTTGAAATC+TGG + chr3_4:48421379-48421398 Msa0504230:CDS 25.0%
!! ATTGCACAACATTTAGAATT+TGG - chr3_4:48421493-48421512 None:intergenic 25.0%
! TATCGATGAAAGAATCTTAT+TGG + chr3_4:48421675-48421694 Msa0504230:intron 25.0%
!! AATACTAGTTAGTCTATGAT+GGG - chr3_4:48421771-48421790 None:intergenic 25.0%
!! AAATACTAGTTAGTCTATGA+TGG - chr3_4:48421772-48421791 None:intergenic 25.0%
!! CTTAGTAAGTATGTGTTTTT+AGG - chr3_4:48421842-48421861 None:intergenic 25.0%
!!! AGTTTTGAATGGTTTTACAT+AGG - chr3_4:48421945-48421964 None:intergenic 25.0%
! ACAAATTTAGCAACTACAAA+AGG + chr3_4:48422463-48422482 Msa0504230:CDS 25.0%
! CAAATTTAGCAACTACAAAA+GGG + chr3_4:48422464-48422483 Msa0504230:CDS 25.0%
! TTAAATCCATGAAGATGAAT+AGG - chr3_4:48422571-48422590 None:intergenic 25.0%
!! GGATTTAATTTCTTTGTAGT+TGG + chr3_4:48422583-48422602 Msa0504230:CDS 25.0%
! TTAATTTCTTTGTAGTTGGT+AGG + chr3_4:48422587-48422606 Msa0504230:CDS 25.0%
!! TAATTTCTTTGTAGTTGGTA+GGG + chr3_4:48422588-48422607 Msa0504230:CDS 25.0%
!! AGGAAACTTTGATTCTAAAA+AGG + chr3_4:48422615-48422634 Msa0504230:CDS 25.0%
! TGTATCCTAATAAATTTACC+TGG - chr3_4:48422736-48422755 None:intergenic 25.0%
!! CACTAATGATTTGTATCAAT+TGG - chr3_4:48422781-48422800 None:intergenic 25.0%
!!! GAAATCTCGATTTTGAAAAT+AGG + chr3_4:48422954-48422973 Msa0504230:intron 25.0%
! ATGTTGTATGAATTTCCAAA+TGG - chr3_4:48423154-48423173 None:intergenic 25.0%
! ATTTGGAAATTCATACAACA+TGG + chr3_4:48423153-48423172 Msa0504230:CDS 25.0%
! TTTGGAAATTCATACAACAT+GGG + chr3_4:48423154-48423173 Msa0504230:CDS 25.0%
! TCCAATAAAATCTTGTCATT+GGG - chr3_4:48423335-48423354 None:intergenic 25.0%
! ATAGACCAATAAATGTCAAT+TGG - chr3_4:48423378-48423397 None:intergenic 25.0%
! ATGAAAAAAACACAACTAAC+AGG - chr3_4:48423409-48423428 None:intergenic 25.0%
CTATAGCAGAATTTCTTTGT+TGG + chr3_4:48419797-48419816 Msa0504230:five_prime_UTR 30.0%
AGGTGTAGAACTGAAAATAA+GGG + chr3_4:48419826-48419845 Msa0504230:five_prime_UTR 30.0%
GTTTCGAATTATACTATTGC+TGG + chr3_4:48419871-48419890 Msa0504230:CDS 30.0%
TAAATTTGGCTAGTGTGTTA+TGG + chr3_4:48420205-48420224 Msa0504230:intron 30.0%
GTCAAACTTAACAAAAAGTG+TGG + chr3_4:48420235-48420254 Msa0504230:intron 30.0%
! TATTGGACCATACTTTTATC+AGG + chr3_4:48420428-48420447 Msa0504230:intron 30.0%
! ATTGGACCATACTTTTATCA+GGG + chr3_4:48420429-48420448 Msa0504230:intron 30.0%
TATAGTTCCATAGATATCCT+TGG + chr3_4:48420651-48420670 Msa0504230:intron 30.0%
TTGAAAACCAAGGATATCTA+TGG - chr3_4:48420661-48420680 None:intergenic 30.0%
TGCTAACATTGTATTTGACA+TGG - chr3_4:48420836-48420855 None:intergenic 30.0%
AATACAATGTTAGCATACAC+TGG + chr3_4:48420841-48420860 Msa0504230:CDS 30.0%
! TGAAATTTTAAACAGGCATG+GGG + chr3_4:48420958-48420977 Msa0504230:intron 30.0%
TGAAATTGTACAAATAGGTC+TGG - chr3_4:48421051-48421070 None:intergenic 30.0%
TTGTACAATTTCACTCTTAC+AGG + chr3_4:48421058-48421077 Msa0504230:CDS 30.0%
AAGCTTAGGTAAAATGACTA+AGG - chr3_4:48421146-48421165 None:intergenic 30.0%
!! TTAGTCATTTTACCTAAGCT+TGG + chr3_4:48421145-48421164 Msa0504230:CDS 30.0%
!! ACAATAACTTGTTCCATATG+TGG - chr3_4:48421193-48421212 None:intergenic 30.0%
!!! TTTTTTGTTTAGGTGAATGG+TGG + chr3_4:48421335-48421354 Msa0504230:intron 30.0%
!!! ATTCAAGTTTTAAGCCACAT+TGG - chr3_4:48421525-48421544 None:intergenic 30.0%
ATTCAAACTTAAGTCTGTGT+GGG - chr3_4:48421650-48421669 None:intergenic 30.0%
AATTCAAACTTAAGTCTGTG+TGG - chr3_4:48421651-48421670 None:intergenic 30.0%
!!! ACAATTGTGTCAGTTTTGAA+TGG - chr3_4:48421956-48421975 None:intergenic 30.0%
GTAACAGCCAAACATAAAAT+TGG + chr3_4:48422007-48422026 Msa0504230:CDS 30.0%
!! GTTTTGTTGTCAACAACAAT+AGG - chr3_4:48422070-48422089 None:intergenic 30.0%
! TCTTTAAAGGAACTTTAGCA+GGG - chr3_4:48422228-48422247 None:intergenic 30.0%
! ATCTTTAAAGGAACTTTAGC+AGG - chr3_4:48422229-48422248 None:intergenic 30.0%
GCACATTTCTTCAAGATTAA+AGG + chr3_4:48422376-48422395 Msa0504230:CDS 30.0%
CTGTGGTTTATAACTTTACA+GGG + chr3_4:48422431-48422450 Msa0504230:CDS 30.0%
AATCATCCTATTCATCTTCA+TGG + chr3_4:48422562-48422581 Msa0504230:CDS 30.0%
!! AATTTCTTTGTAGTTGGTAG+GGG + chr3_4:48422589-48422608 Msa0504230:CDS 30.0%
GTGTTGTGCTAATTAAAGTA+TGG + chr3_4:48423093-48423112 Msa0504230:intron 30.0%
! ATTATATTTTGTTAGGGGTG+TGG + chr3_4:48423117-48423136 Msa0504230:intron 30.0%
TTGGAAATTCATACAACATG+GGG + chr3_4:48423155-48423174 Msa0504230:CDS 30.0%
ATATGACACTGAGTGTTAAT+TGG - chr3_4:48423274-48423293 None:intergenic 30.0%
CTCCAATAAAATCTTGTCAT+TGG - chr3_4:48423336-48423355 None:intergenic 30.0%
GATGTCCAATTGACATTTAT+TGG + chr3_4:48423370-48423389 Msa0504230:three_prime_UTR 30.0%
AATTTCTTTGTTGGGTAGAG+AGG + chr3_4:48419806-48419825 Msa0504230:five_prime_UTR 35.0%
GAGGTGTAGAACTGAAAATA+AGG + chr3_4:48419825-48419844 Msa0504230:five_prime_UTR 35.0%
GGTGTAGAACTGAAAATAAG+GGG + chr3_4:48419827-48419846 Msa0504230:five_prime_UTR 35.0%
! ACAAAAAGTGTGGATACACA+AGG + chr3_4:48420245-48420264 Msa0504230:intron 35.0%
! AATCATGTCGATCGTGATTT+TGG + chr3_4:48420308-48420327 Msa0504230:intron 35.0%
GGCTGATAAGTTTGAATAGA+CGG - chr3_4:48420360-48420379 None:intergenic 35.0%
ACAGCTCAATCGAAGAATAT+TGG - chr3_4:48420381-48420400 None:intergenic 35.0%
GACGGCGAATTAAAATGTAT+TGG + chr3_4:48420411-48420430 Msa0504230:intron 35.0%
TTAGCTCCCTGATAAAAGTA+TGG - chr3_4:48420438-48420457 None:intergenic 35.0%
! TGCTGTTTGGTAGATATTCA+TGG - chr3_4:48420579-48420598 None:intergenic 35.0%
TATCTACCAAACAGCAAAGT+AGG + chr3_4:48420583-48420602 Msa0504230:intron 35.0%
CAAGGCAAGAGACTTATATT+TGG + chr3_4:48420621-48420640 Msa0504230:intron 35.0%
TGTCACATTCTTGAAAACCA+AGG - chr3_4:48420671-48420690 None:intergenic 35.0%
TTTGGTTGAACACAATCTTG+TGG - chr3_4:48420730-48420749 None:intergenic 35.0%
TAGAACATTGTCATCTTCTC+TGG - chr3_4:48420799-48420818 None:intergenic 35.0%
ACTTGTTCCATATGTGGTTT+GGG - chr3_4:48421187-48421206 None:intergenic 35.0%
! AACTTGTTCCATATGTGGTT+TGG - chr3_4:48421188-48421207 None:intergenic 35.0%
!! TGAAGCTTTGAAATCTGGAT+TGG + chr3_4:48421384-48421403 Msa0504230:CDS 35.0%
GCAATTTGACTAAACCAATG+TGG + chr3_4:48421508-48421527 Msa0504230:intron 35.0%
! ATTATTCGTCACTTTACCCA+CGG + chr3_4:48421598-48421617 Msa0504230:intron 35.0%
TTACAAGCACATTTGTGGTT+TGG - chr3_4:48421991-48422010 None:intergenic 35.0%
AACTTTAGCAGGGAATTTCT+TGG - chr3_4:48422218-48422237 None:intergenic 35.0%
!! TTGCTTTTCACTGTTTCTCT+TGG + chr3_4:48422259-48422278 Msa0504230:CDS 35.0%
GTAAAGTTATAAACCACAGG+AGG - chr3_4:48422430-48422449 None:intergenic 35.0%
CCTGTAAAGTTATAAACCAC+AGG - chr3_4:48422433-48422452 None:intergenic 35.0%
CCTGTGGTTTATAACTTTAC+AGG + chr3_4:48422430-48422449 Msa0504230:CDS 35.0%
!!! CAATTGGTTTTGCAAGATAC+TGG + chr3_4:48422523-48422542 Msa0504230:CDS 35.0%
AGATGAATAGGATGATTCTC+AGG - chr3_4:48422559-48422578 None:intergenic 35.0%
! TTTGTTGTGGACAATGGTAA+AGG + chr3_4:48423191-48423210 Msa0504230:CDS 35.0%
GGTGGTAATAGTGATTCATT+TGG - chr3_4:48423218-48423237 None:intergenic 35.0%
TGGTACATTTCTTAGCACTT+GGG - chr3_4:48423254-48423273 None:intergenic 35.0%
TTGGTACATTTCTTAGCACT+TGG - chr3_4:48423255-48423274 None:intergenic 35.0%
! GCCCAATGACAAGATTTTAT+TGG + chr3_4:48423331-48423350 Msa0504230:three_prime_UTR 35.0%
TTCTGATGCAACAAAGACAA+GGG - chr3_4:48423523-48423542 None:intergenic 35.0%
!! ACTGAAAATAAGGGGTGTCA+TGG + chr3_4:48419835-48419854 Msa0504230:exon 40.0%
ATGTTTGCTTCCTTTGTCTG+TGG + chr3_4:48419898-48419917 Msa0504230:CDS 40.0%
CGACACTACAAGTTCAATGT+AGG + chr3_4:48419932-48419951 Msa0504230:intron 40.0%
!! TGTGGATACACAAGGCATTT+TGG + chr3_4:48420253-48420272 Msa0504230:intron 40.0%
TGAGCTGTAGATCATCATGA+CGG + chr3_4:48420393-48420412 Msa0504230:intron 40.0%
ACAGTAAATGGAAAGTTCCC+AGG + chr3_4:48420759-48420778 Msa0504230:CDS 40.0%
TCTTCTCTGGCATAGATTGT+AGG - chr3_4:48420786-48420805 None:intergenic 40.0%
TTCACTCTTACAGGTCAAAG+AGG + chr3_4:48421067-48421086 Msa0504230:CDS 40.0%
! GTAAAATGACTAAGGCACCA+TGG - chr3_4:48421138-48421157 None:intergenic 40.0%
! CCATATGTGGTTTGGGAAAA+GGG - chr3_4:48421180-48421199 None:intergenic 40.0%
! TCCATATGTGGTTTGGGAAA+AGG - chr3_4:48421181-48421200 None:intergenic 40.0%
! ATGGTGGAAATCAGATACTG+AGG + chr3_4:48421351-48421370 Msa0504230:CDS 40.0%
ATTGATTGTGTGAGCATCAG+AGG - chr3_4:48421419-48421438 None:intergenic 40.0%
TCTGATGCTCACACAATCAA+TGG + chr3_4:48421418-48421437 Msa0504230:CDS 40.0%
ACACAATCAATGGTCATCCA+GGG + chr3_4:48421428-48421447 Msa0504230:CDS 40.0%
! TTTGAGTACGAGGGATTTTC+CGG + chr3_4:48421730-48421749 Msa0504230:intron 40.0%
TGGGAGAAATTAACACGATC+CGG - chr3_4:48421752-48421771 None:intergenic 40.0%
!!! CAACTTTTTTGTGTGCATGC+AGG + chr3_4:48421794-48421813 Msa0504230:intron 40.0%
CCTCAACAACAGTTAGTTGA+TGG - chr3_4:48421913-48421932 None:intergenic 40.0%
CCATCAACTAACTGTTGTTG+AGG + chr3_4:48421910-48421929 Msa0504230:CDS 40.0%
GACACAATTGTTATAGCACC+TGG + chr3_4:48421965-48421984 Msa0504230:CDS 40.0%
ACAATAGGTGCATCCATGAA+AGG - chr3_4:48422055-48422074 None:intergenic 40.0%
TGAATAGTGTAAAGTGGCAG+TGG - chr3_4:48422095-48422114 None:intergenic 40.0%
!! ACTGCCACTTTACACTATTC+AGG + chr3_4:48422094-48422113 Msa0504230:CDS 40.0%
TGTGCCTGAATAGTGTAAAG+TGG - chr3_4:48422101-48422120 None:intergenic 40.0%
TTACACTATTCAGGCACACT+TGG + chr3_4:48422103-48422122 Msa0504230:CDS 40.0%
AGAAGTTAAGGTTGTGAGTG+AGG - chr3_4:48422131-48422150 None:intergenic 40.0%
TGTAGGAGGCAAAGAAGTTA+AGG - chr3_4:48422143-48422162 None:intergenic 40.0%
ACTGAAGTAGCATTTGTAGG+AGG - chr3_4:48422157-48422176 None:intergenic 40.0%
GCAACTGAAGTAGCATTTGT+AGG - chr3_4:48422160-48422179 None:intergenic 40.0%
TTGTTCACACAAGTAGCACA+AGG - chr3_4:48422292-48422311 None:intergenic 40.0%
! GTTTTTACTGACGACTTTCC+TGG + chr3_4:48422400-48422419 Msa0504230:CDS 40.0%
TAGCAACTACAAAAGGGACA+AGG + chr3_4:48422470-48422489 Msa0504230:CDS 40.0%
GTTCAATCTTGTTGATCCTG+TGG + chr3_4:48422648-48422667 Msa0504230:CDS 40.0%
TTGGACTGCTATCAGATTCA+GGG + chr3_4:48422702-48422721 Msa0504230:CDS 40.0%
GTGGTTTATGCATTGCCATT+TGG + chr3_4:48423136-48423155 Msa0504230:CDS 40.0%
! ACTGAAGATGGCATTTGTTG+TGG + chr3_4:48423178-48423197 Msa0504230:CDS 40.0%
!! ATGGCATTTGTTGTGGACAA+TGG + chr3_4:48423185-48423204 Msa0504230:CDS 40.0%
GGTACATTTCTTAGCACTTG+GGG - chr3_4:48423253-48423272 None:intergenic 40.0%
TCTGATGCAACAAAGACAAG+GGG - chr3_4:48423522-48423541 None:intergenic 40.0%
CTTCTGATGCAACAAAGACA+AGG - chr3_4:48423524-48423543 None:intergenic 40.0%
!! GAAAATAAGGGGTGTCATGG+CGG + chr3_4:48419838-48419857 Msa0504230:exon 45.0%
TCCTTTGTCTGTGGATGCTA+TGG + chr3_4:48419907-48419926 Msa0504230:CDS 45.0%
ACCATAGCATCCACAGACAA+AGG - chr3_4:48419911-48419930 None:intergenic 45.0%
!! CCTACACCTACTTTGCTGTT+TGG - chr3_4:48420592-48420611 None:intergenic 45.0%
CCAAACAGCAAAGTAGGTGT+AGG + chr3_4:48420589-48420608 Msa0504230:intron 45.0%
AGGTGTAGGATTGATGAGCA+AGG + chr3_4:48420603-48420622 Msa0504230:intron 45.0%
TGGCATAGATTGTAGGACCT+GGG - chr3_4:48420779-48420798 None:intergenic 45.0%
TGGGGTCAAACAACTACGAA+CGG + chr3_4:48420976-48420995 Msa0504230:CDS 45.0%
TCAAACAACTACGAACGGGT+TGG + chr3_4:48420981-48421000 Msa0504230:CDS 45.0%
! CAAACAACTACGAACGGGTT+GGG + chr3_4:48420982-48421001 Msa0504230:CDS 45.0%
TTGTACAAATAGGTCTGGCC+CGG - chr3_4:48421046-48421065 None:intergenic 45.0%
! ATGCTCATGTCCTTTGGCTT+AGG + chr3_4:48421104-48421123 Msa0504230:CDS 45.0%
! TGCTCATGTCCTTTGGCTTA+GGG + chr3_4:48421105-48421124 Msa0504230:CDS 45.0%
! TGTGGTTTGGGAAAAGGGTA+AGG - chr3_4:48421175-48421194 None:intergenic 45.0%
! CCCTTTTCCCAAACCACATA+TGG + chr3_4:48421177-48421196 Msa0504230:CDS 45.0%
!! GTGTGAGCATCAGAGGTATT+TGG - chr3_4:48421412-48421431 None:intergenic 45.0%
CACACAATCAATGGTCATCC+AGG + chr3_4:48421427-48421446 Msa0504230:CDS 45.0%
!! TGTGAAGCACAGCTTTGGAT+AGG - chr3_4:48421454-48421473 None:intergenic 45.0%
ATCCAAAGCTGTGCTTCACA+AGG + chr3_4:48421454-48421473 Msa0504230:CDS 45.0%
TCATCGCTCTTTGAGTACGA+GGG + chr3_4:48421721-48421740 Msa0504230:intron 45.0%
!!! CTTTTTTGTGTGCATGCAGG+AGG + chr3_4:48421797-48421816 Msa0504230:intron 45.0%
GGCTGTTACAAGCACATTTG+TGG - chr3_4:48421996-48422015 None:intergenic 45.0%
AGTTGCAGCTTCTCCTTTCA+TGG + chr3_4:48422039-48422058 Msa0504230:CDS 45.0%
GAAATGTGCTTGAAGAAGCG+CGG - chr3_4:48422365-48422384 None:intergenic 45.0%
TAAACCACAGGAGGATTTCC+AGG - chr3_4:48422421-48422440 None:intergenic 45.0%
!! TTTGTAGTTGGTAGGGGACA+AGG + chr3_4:48422595-48422614 Msa0504230:CDS 45.0%
ATCTTGTTGATCCTGTGGAG+AGG + chr3_4:48422653-48422672 Msa0504230:CDS 45.0%
AATACAGTAGGTGTTCCAGC+TGG + chr3_4:48422676-48422695 Msa0504230:CDS 45.0%
GTTGGACTGCTATCAGATTC+AGG + chr3_4:48422701-48422720 Msa0504230:CDS 45.0%
AGATTCAGGGCTGATAATCC+AGG + chr3_4:48422715-48422734 Msa0504230:CDS 45.0%
TACAACATGGGGACTGAAGA+TGG + chr3_4:48423166-48423185 Msa0504230:CDS 45.0%
ACATTTCTTAGCACTTGGGG+AGG - chr3_4:48423250-48423269 None:intergenic 45.0%
!!! TTAATTATTTTTTTTTGTTT+AGG + chr3_4:48421325-48421344 Msa0504230:intron 5.0%
CTGGCATAGATTGTAGGACC+TGG - chr3_4:48420780-48420799 None:intergenic 50.0%
GGGGTCAAACAACTACGAAC+GGG + chr3_4:48420977-48420996 Msa0504230:CDS 50.0%
GGTTATGTATGCTGGCCCAT+CGG - chr3_4:48421008-48421027 None:intergenic 50.0%
!! GGGCATTGGGTTATGTATGC+TGG - chr3_4:48421016-48421035 None:intergenic 50.0%
AACCCAATGCCCGATTCAAC+CGG + chr3_4:48421024-48421043 Msa0504230:CDS 50.0%
! CAGGTCAAAGAGGCACACTT+TGG + chr3_4:48421077-48421096 Msa0504230:CDS 50.0%
! GTCAAAGAGGCACACTTTGG+TGG + chr3_4:48421080-48421099 Msa0504230:CDS 50.0%
GGGTAAGGAACTCCAAGCTT+AGG - chr3_4:48421160-48421179 None:intergenic 50.0%
CACCTTGTGAAGCACAGCTT+TGG - chr3_4:48421459-48421478 None:intergenic 50.0%
! GTCACTTTACCCACGGAGAT+TGG + chr3_4:48421605-48421624 Msa0504230:intron 50.0%
CCAACTAGACCAATCTCCGT+GGG - chr3_4:48421617-48421636 None:intergenic 50.0%
ACCAACTAGACCAATCTCCG+TGG - chr3_4:48421618-48421637 None:intergenic 50.0%
GTCATCGCTCTTTGAGTACG+AGG + chr3_4:48421720-48421739 Msa0504230:intron 50.0%
GCATGCAGGAGGATTCTCAT+TGG + chr3_4:48421808-48421827 Msa0504230:intron 50.0%
! AGCACATTTGTGGTTTGGCC+AGG - chr3_4:48421986-48422005 None:intergenic 50.0%
!! GCTTGAAGAAGCGCGGTTTT+CGG - chr3_4:48422358-48422377 None:intergenic 50.0%
CTTTCCTGGAAATCCTCCTG+TGG + chr3_4:48422414-48422433 Msa0504230:CDS 50.0%
CCTACTGTATTCCTCTCCAC+AGG - chr3_4:48422667-48422686 None:intergenic 50.0%
CCTGTGGAGAGGAATACAGT+AGG + chr3_4:48422664-48422683 Msa0504230:CDS 50.0%
!! AATAAGGGGTGTCATGGCGG+TGG + chr3_4:48419841-48419860 Msa0504230:exon 55.0%
ACCCAATGCCCGATTCAACC+GGG + chr3_4:48421025-48421044 Msa0504230:CDS 55.0%
TAGGTCTGGCCCGGTTGAAT+CGG - chr3_4:48421037-48421056 None:intergenic 55.0%
GGTGGCATGCTCATGTCCTT+TGG + chr3_4:48421098-48421117 Msa0504230:CDS 55.0%
! GACTAAGGCACCATGGACAG+TGG - chr3_4:48421131-48421150 None:intergenic 55.0%
!! CCCACGGAGATTGGTCTAGT+TGG + chr3_4:48421614-48421633 Msa0504230:intron 55.0%
ACAGTAGGTGTTCCAGCTGG+AGG + chr3_4:48422679-48422698 Msa0504230:CDS 55.0%
TAGGTGTTCCAGCTGGAGGT+TGG + chr3_4:48422683-48422702 Msa0504230:CDS 55.0%
ATAGCAGTCCAACCTCCAGC+TGG - chr3_4:48422694-48422713 None:intergenic 55.0%
TAGCACTTGGGGAGGTCACT+TGG - chr3_4:48423242-48423261 None:intergenic 55.0%
!! TACGAACGGGTTGGGCCGAT+GGG + chr3_4:48420990-48421009 Msa0504230:CDS 60.0%
GCCCGGTTGAATCGGGCATT+GGG - chr3_4:48421029-48421048 None:intergenic 60.0%
AGGTCTGGCCCGGTTGAATC+GGG - chr3_4:48421036-48421055 None:intergenic 60.0%
GGACAGTGGCCCTAAGCCAA+AGG - chr3_4:48421117-48421136 None:intergenic 60.0%
TGGCTTAGGGCCACTGTCCA+TGG + chr3_4:48421118-48421137 Msa0504230:CDS 60.0%
!! GCACAGCTTTGGATAGGCCC+TGG - chr3_4:48421448-48421467 None:intergenic 60.0%
TTGGGGAGGTCACTTGGAGG+TGG - chr3_4:48423236-48423255 None:intergenic 60.0%
CACTTGGGGAGGTCACTTGG+AGG - chr3_4:48423239-48423258 None:intergenic 60.0%
! GGGTGTCATGGCGGTGGTTG+CGG + chr3_4:48419847-48419866 Msa0504230:exon 65.0%
!! CTACGAACGGGTTGGGCCGA+TGG + chr3_4:48420989-48421008 Msa0504230:CDS 65.0%
GGCCCGGTTGAATCGGGCAT+TGG - chr3_4:48421030-48421049 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 48419750 48423587 48419750 ID=Msa0504230;Name=Msa0504230
chr3_4 mRNA 48419750 48423587 48419750 ID=Msa0504230-mRNA-1;Parent=Msa0504230;Name=Msa0504230-mRNA-1;_AED=0.08;_eAED=0.08;_QI=104|1|1|1|1|1|6|325|557
chr3_4 exon 48419750 48419949 48419750 ID=Msa0504230-mRNA-1:exon:10418;Parent=Msa0504230-mRNA-1
chr3_4 exon 48420711 48420862 48420711 ID=Msa0504230-mRNA-1:exon:10419;Parent=Msa0504230-mRNA-1
chr3_4 exon 48420973 48421217 48420973 ID=Msa0504230-mRNA-1:exon:10420;Parent=Msa0504230-mRNA-1
chr3_4 exon 48421347 48421475 48421347 ID=Msa0504230-mRNA-1:exon:10421;Parent=Msa0504230-mRNA-1
chr3_4 exon 48421816 48422736 48421816 ID=Msa0504230-mRNA-1:exon:10422;Parent=Msa0504230-mRNA-1
chr3_4 exon 48423132 48423587 48423132 ID=Msa0504230-mRNA-1:exon:10423;Parent=Msa0504230-mRNA-1
chr3_4 five_prime_UTR 48419750 48419853 48419750 ID=Msa0504230-mRNA-1:five_prime_utr;Parent=Msa0504230-mRNA-1
chr3_4 CDS 48419854 48419949 48419854 ID=Msa0504230-mRNA-1:cds;Parent=Msa0504230-mRNA-1
chr3_4 CDS 48420711 48420862 48420711 ID=Msa0504230-mRNA-1:cds;Parent=Msa0504230-mRNA-1
chr3_4 CDS 48420973 48421217 48420973 ID=Msa0504230-mRNA-1:cds;Parent=Msa0504230-mRNA-1
chr3_4 CDS 48421347 48421475 48421347 ID=Msa0504230-mRNA-1:cds;Parent=Msa0504230-mRNA-1
chr3_4 CDS 48421816 48422736 48421816 ID=Msa0504230-mRNA-1:cds;Parent=Msa0504230-mRNA-1
chr3_4 CDS 48423132 48423262 48423132 ID=Msa0504230-mRNA-1:cds;Parent=Msa0504230-mRNA-1
chr3_4 three_prime_UTR 48423263 48423587 48423263 ID=Msa0504230-mRNA-1:three_prime_utr;Parent=Msa0504230-mRNA-1
Gene Sequence

>Msa0504230

ATGGCGGTGGTTGCGGCGTTTCGAATTATACTATTGCTGGCTGCATGTTTGCTTCCTTTGTCTGTGGATGCTATGGTTCGACACTACAAGTTCAATGTTGTGATGAAAAATGCCACAAGATTGTGTTCAACCAAACCAATTGTAACAGTAAATGGAAAGTTCCCAGGTCCTACAATCTATGCCAGAGAAGATGACAATGTTCTAATAAAGGTTGTCAACCATGTCAAATACAATGTTAGCATACACTGGCATGGGGTCAAACAACTACGAACGGGTTGGGCCGATGGGCCAGCATACATAACCCAATGCCCGATTCAACCGGGCCAGACCTATTTGTACAATTTCACTCTTACAGGTCAAAGAGGCACACTTTGGTGGCATGCTCATGTCCTTTGGCTTAGGGCCACTGTCCATGGTGCCTTAGTCATTTTACCTAAGCTTGGAGTTCCTTACCCTTTTCCCAAACCACATATGGAACAAGTTATTGTATTAGGTGAATGGTGGAAATCAGATACTGAGGCTATAATAAATGAAGCTTTGAAATCTGGATTGGCTCCAAATACCTCTGATGCTCACACAATCAATGGTCATCCAGGGCCTATCCAAAGCTGTGCTTCACAAGGAGGATTCTCATTGGAAGTACAACCTAAAAACACATACTTACTAAGAATCATCAATGCTGCACTCAATGAAGAGCTCTTCTTTAAAATTGCTAACCATCAACTAACTGTTGTTGAGGTTGATGCAACCTATGTAAAACCATTCAAAACTGACACAATTGTTATAGCACCTGGCCAAACCACAAATGTGCTTGTAACAGCCAAACATAAAATTGGAAATTTCTTAGTTGCAGCTTCTCCTTTCATGGATGCACCTATTGTTGTTGACAACAAAACTGCCACTGCCACTTTACACTATTCAGGCACACTTGGTTCCTCACTCACAACCTTAACTTCTTTGCCTCCTACAAATGCTACTTCAGTTGCTAACACTTTCACAGATTCTCTAAGAAGCTTAAACTCCAAGAAATTCCCTGCTAAAGTTCCTTTAAAGATTGATCATAACTTGCTTTTCACTGTTTCTCTTGGTGTCAATCCTTGTGCTACTTGTGTGAACAATAGTCGCGTCGTAGCTGATATCAACAATGTTACATTTGTGATGCCGAAAACCGCGCTTCTTCAAGCACATTTCTTCAAGATTAAAGGAGTTTTTACTGACGACTTTCCTGGAAATCCTCCTGTGGTTTATAACTTTACAGGGTCACAAGTGACAAATTTAGCAACTACAAAAGGGACAAGGCTTTATAGACTTGCTTATAATTCAACAGTTCAATTGGTTTTGCAAGATACTGGAATGTTAACACCTGAGAATCATCCTATTCATCTTCATGGATTTAATTTCTTTGTAGTTGGTAGGGGACAAGGAAACTTTGATTCTAAAAAGGATGTAAAAAAGTTCAATCTTGTTGATCCTGTGGAGAGGAATACAGTAGGTGTTCCAGCTGGAGGTTGGACTGCTATCAGATTCAGGGCTGATAATCCAGGGGTGTGGTTTATGCATTGCCATTTGGAAATTCATACAACATGGGGACTGAAGATGGCATTTGTTGTGGACAATGGTAAAGGACCAAATGAATCACTATTACCACCTCCAAGTGACCTCCCCAAGTGCTAA
Protein sequence

>Msa0504230

MAVVAAFRIILLLAACLLPLSVDAMVRHYKFNVVMKNATRLCSTKPIVTVNGKFPGPTIYAREDDNVLIKVVNHVKYNVSIHWHGVKQLRTGWADGPAYITQCPIQPGQTYLYNFTLTGQRGTLWWHAHVLWLRATVHGALVILPKLGVPYPFPKPHMEQVIVLGEWWKSDTEAIINEALKSGLAPNTSDAHTINGHPGPIQSCASQGGFSLEVQPKNTYLLRIINAALNEELFFKIANHQLTVVEVDATYVKPFKTDTIVIAPGQTTNVLVTAKHKIGNFLVAASPFMDAPIVVDNKTATATLHYSGTLGSSLTTLTSLPPTNATSVANTFTDSLRSLNSKKFPAKVPLKIDHNLLFTVSLGVNPCATCVNNSRVVADINNVTFVMPKTALLQAHFFKIKGVFTDDFPGNPPVVYNFTGSQVTNLATTKGTRLYRLAYNSTVQLVLQDTGMLTPENHPIHLHGFNFFVVGRGQGNFDSKKDVKKFNLVDPVERNTVGVPAGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFVVDNGKGPNESLLPPPSDLPKC*