AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0504370


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0504370 MtrunA17_Chr3g0104461 79.752 242 34 1 1 227 6 247 2.38e-139 391
Msa0504370 MtrunA17_Chr6g0477881 32.394 142 68 3 13 127 282 422 8.98e-12 64.3
Msa0504370 MtrunA17_Chr6g0477921 32.394 142 68 3 13 127 282 422 9.34e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 40 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATCGAACTTATCATATTT+TGG 0.135203 3_4:+48665621 None:intergenic
TACTTCTCGACACCAATTTA+AGG 0.181120 3_4:-48665550 Msa0504370:CDS
CGGCAGATACTTCTTGATTC+TGG 0.254435 3_4:+48665022 None:intergenic
GATACTGAATTTGAAATCTT+TGG 0.280623 3_4:+48665584 None:intergenic
CTCGAACAGCGTCCTTAAAT+TGG 0.286125 3_4:+48665538 None:intergenic
TTTCTCTTTGCCGCTTCATT+TGG 0.322340 3_4:+48665132 None:intergenic
CGAGAAGTTGTCAAATGAAT+TGG 0.331298 3_4:-48665682 Msa0504370:CDS
TTCCAGGTCTAAGTGGATTA+AGG 0.335256 3_4:-48665325 Msa0504370:CDS
TGAATTGGACAATGCATCTA+AGG 0.342142 3_4:-48665667 Msa0504370:CDS
AGAGAGTTAGAGCTGTTTGT+TGG 0.416562 3_4:-48665464 Msa0504370:CDS
TGTTAAATTTCAAGAGTTCC+AGG 0.422642 3_4:-48665341 Msa0504370:CDS
CAACTAGAAGTGCTTGTTGA+TGG 0.433969 3_4:-48665057 Msa0504370:CDS
TAAGGAAGATTCTGAAATGA+AGG 0.442909 3_4:-48665649 Msa0504370:CDS
ACATAAGATGGTACATGGTA+AGG 0.453946 3_4:+48664925 None:intergenic
ACAAGGTTCACTGCAGAAAT+CGG 0.464428 3_4:+48664964 None:intergenic
TTGCTTAGAGTATTTCCCTT+AGG 0.472524 3_4:+48665162 None:intergenic
GTTATATCAGGGGAGGCCTA+AGG 0.479297 3_4:-48665178 Msa0504370:intron
AACTAGAAGTGCTTGTTGAT+GGG 0.480143 3_4:-48665056 Msa0504370:CDS
GGTCAGCAGTTGTTGGTACA+AGG 0.494932 3_4:+48664947 None:intergenic
AAAGATTTCAAATTCAGTAT+CGG 0.522915 3_4:-48665582 Msa0504370:CDS
TTTCAAATTCAGTATCGGAA+TGG 0.533351 3_4:-48665577 Msa0504370:CDS
ACTTCTTGATTCTGGTTGAG+AGG 0.533490 3_4:+48665030 None:intergenic
TCAAGAGTTCCAGGTCTAAG+TGG 0.550516 3_4:-48665332 Msa0504370:CDS
ATATGAAGACTCACATAAGA+TGG 0.557045 3_4:+48664913 None:intergenic
CATAAGATGGTACATGGTAA+GGG 0.562570 3_4:+48664926 None:intergenic
ACTAGAAGTGCTTGTTGATG+GGG 0.564459 3_4:-48665055 Msa0504370:CDS
TTATATCAGGGGAGGCCTAA+GGG 0.564632 3_4:-48665177 Msa0504370:intron
ATCTGCCGATGACGATATGT+TGG 0.564735 3_4:-48665007 Msa0504370:CDS
TTCCTTAATCCACTTAGACC+TGG 0.569538 3_4:+48665323 None:intergenic
TTTCGCCAACATATCGTCAT+CGG 0.574686 3_4:+48665002 None:intergenic
AGACTAATACATTTAGGGTG+AGG 0.581685 3_4:-48665395 Msa0504370:CDS
CTACATCCGATGCATACTTG+TGG 0.590463 3_4:-48665369 Msa0504370:CDS
GACTCACATAAGATGGTACA+TGG 0.591591 3_4:+48664920 None:intergenic
TGGTAAGGGTCAGCAGTTGT+TGG 0.600697 3_4:+48664940 None:intergenic
AAGAAGCACAGTGTGTGGAA+TGG 0.600857 3_4:-48665709 Msa0504370:CDS
ACCATTCCACAAGTATGCAT+CGG 0.606989 3_4:+48665363 None:intergenic
AAGGAAGATTCTGAAATGAA+GGG 0.614609 3_4:-48665648 Msa0504370:CDS
CAATGTTAAACCAAATGAAG+CGG 0.616971 3_4:-48665142 Msa0504370:CDS
TTATTAAGAAGCACAGTGTG+TGG 0.620202 3_4:-48665714 Msa0504370:CDS
TCCGATGCATACTTGTGGAA+TGG 0.644401 3_4:-48665364 Msa0504370:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGATTTCAAATTCAGTAT+CGG - chr3_4:48665021-48665040 Msa0504370:CDS 20.0%
!! AAAATAAAATTTACCAGGAA+TGG - chr3_4:48665111-48665130 Msa0504370:CDS 20.0%
!!! AAACATTGTATGAACATTTT+TGG + chr3_4:48665170-48665189 None:intergenic 20.0%
!! GAAAAAGACTAATACATTTA+GGG - chr3_4:48665203-48665222 Msa0504370:intron 20.0%
!!! TATAAAATTACCCTCTTTTT+AGG + chr3_4:48665336-48665355 None:intergenic 20.0%
!! AAAGATTTCAAATTCAGTAT+CGG - chr3_4:48665021-48665040 Msa0504370:CDS 20.0%
!! AAAATAAAATTTACCAGGAA+TGG - chr3_4:48665111-48665130 Msa0504370:CDS 20.0%
!!! AAACATTGTATGAACATTTT+TGG + chr3_4:48665170-48665189 None:intergenic 20.0%
!! GAAAAAGACTAATACATTTA+GGG - chr3_4:48665203-48665222 Msa0504370:intron 20.0%
!!! TATAAAATTACCCTCTTTTT+AGG + chr3_4:48665336-48665355 None:intergenic 20.0%
!! GATACTGAATTTGAAATCTT+TGG + chr3_4:48665022-48665041 None:intergenic 25.0%
! GCAGAAAAATAAAATTTACC+AGG - chr3_4:48665106-48665125 Msa0504370:CDS 25.0%
! CGAAAAAGACTAATACATTT+AGG - chr3_4:48665202-48665221 Msa0504370:intron 25.0%
!!! GTTCTTTTTGTTTTTGTGTT+TGG - chr3_4:48665378-48665397 Msa0504370:CDS 25.0%
!!! CTAATGTGTTTGTTATATCA+GGG - chr3_4:48665414-48665433 Msa0504370:CDS 25.0%
!!! TAATGTGTTTGTTATATCAG+GGG - chr3_4:48665415-48665434 Msa0504370:CDS 25.0%
!! GATACTGAATTTGAAATCTT+TGG + chr3_4:48665022-48665041 None:intergenic 25.0%
! GCAGAAAAATAAAATTTACC+AGG - chr3_4:48665106-48665125 Msa0504370:CDS 25.0%
! CGAAAAAGACTAATACATTT+AGG - chr3_4:48665202-48665221 Msa0504370:intron 25.0%
!!! GTTCTTTTTGTTTTTGTGTT+TGG - chr3_4:48665378-48665397 Msa0504370:CDS 25.0%
!!! CTAATGTGTTTGTTATATCA+GGG - chr3_4:48665414-48665433 Msa0504370:CDS 25.0%
!!! TAATGTGTTTGTTATATCAG+GGG - chr3_4:48665415-48665434 Msa0504370:CDS 25.0%
!! TAAGGAAGATTCTGAAATGA+AGG - chr3_4:48664954-48664973 Msa0504370:CDS 30.0%
!! AAGGAAGATTCTGAAATGAA+GGG - chr3_4:48664955-48664974 Msa0504370:CDS 30.0%
! GCATCGAACTTATCATATTT+TGG + chr3_4:48664985-48665004 None:intergenic 30.0%
! TTTCAAATTCAGTATCGGAA+TGG - chr3_4:48665026-48665045 Msa0504370:CDS 30.0%
TGTTAAATTTCAAGAGTTCC+AGG - chr3_4:48665262-48665281 Msa0504370:intron 30.0%
TCAATCAAGAACCTAAAAAG+AGG - chr3_4:48665322-48665341 Msa0504370:CDS 30.0%
CAATCAAGAACCTAAAAAGA+GGG - chr3_4:48665323-48665342 Msa0504370:CDS 30.0%
!! GCTAATGTGTTTGTTATATC+AGG - chr3_4:48665413-48665432 Msa0504370:CDS 30.0%
CAATGTTAAACCAAATGAAG+CGG - chr3_4:48665461-48665480 Msa0504370:CDS 30.0%
ATATGAAGACTCACATAAGA+TGG + chr3_4:48665693-48665712 None:intergenic 30.0%
!! TAAGGAAGATTCTGAAATGA+AGG - chr3_4:48664954-48664973 Msa0504370:CDS 30.0%
!! AAGGAAGATTCTGAAATGAA+GGG - chr3_4:48664955-48664974 Msa0504370:CDS 30.0%
! GCATCGAACTTATCATATTT+TGG + chr3_4:48664985-48665004 None:intergenic 30.0%
! TTTCAAATTCAGTATCGGAA+TGG - chr3_4:48665026-48665045 Msa0504370:CDS 30.0%
TGTTAAATTTCAAGAGTTCC+AGG - chr3_4:48665262-48665281 Msa0504370:intron 30.0%
TCAATCAAGAACCTAAAAAG+AGG - chr3_4:48665322-48665341 Msa0504370:CDS 30.0%
CAATCAAGAACCTAAAAAGA+GGG - chr3_4:48665323-48665342 Msa0504370:CDS 30.0%
!! GCTAATGTGTTTGTTATATC+AGG - chr3_4:48665413-48665432 Msa0504370:CDS 30.0%
CAATGTTAAACCAAATGAAG+CGG - chr3_4:48665461-48665480 Msa0504370:CDS 30.0%
ATATGAAGACTCACATAAGA+TGG + chr3_4:48665693-48665712 None:intergenic 30.0%
! TTATTAAGAAGCACAGTGTG+TGG - chr3_4:48664889-48664908 Msa0504370:CDS 35.0%
CGAGAAGTTGTCAAATGAAT+TGG - chr3_4:48664921-48664940 Msa0504370:CDS 35.0%
TGAATTGGACAATGCATCTA+AGG - chr3_4:48664936-48664955 Msa0504370:CDS 35.0%
TACTTCTCGACACCAATTTA+AGG - chr3_4:48665053-48665072 Msa0504370:CDS 35.0%
CGAGAGCTCAATGTATTAAA+TGG - chr3_4:48665084-48665103 Msa0504370:CDS 35.0%
! AGACTAATACATTTAGGGTG+AGG - chr3_4:48665208-48665227 Msa0504370:intron 35.0%
! TTGCTTAGAGTATTTCCCTT+AGG + chr3_4:48665444-48665463 None:intergenic 35.0%
!! AACTAGAAGTGCTTGTTGAT+GGG - chr3_4:48665547-48665566 Msa0504370:CDS 35.0%
CATAAGATGGTACATGGTAA+GGG + chr3_4:48665680-48665699 None:intergenic 35.0%
ACATAAGATGGTACATGGTA+AGG + chr3_4:48665681-48665700 None:intergenic 35.0%
! TTATTAAGAAGCACAGTGTG+TGG - chr3_4:48664889-48664908 Msa0504370:CDS 35.0%
CGAGAAGTTGTCAAATGAAT+TGG - chr3_4:48664921-48664940 Msa0504370:CDS 35.0%
TGAATTGGACAATGCATCTA+AGG - chr3_4:48664936-48664955 Msa0504370:CDS 35.0%
TACTTCTCGACACCAATTTA+AGG - chr3_4:48665053-48665072 Msa0504370:CDS 35.0%
CGAGAGCTCAATGTATTAAA+TGG - chr3_4:48665084-48665103 Msa0504370:CDS 35.0%
! AGACTAATACATTTAGGGTG+AGG - chr3_4:48665208-48665227 Msa0504370:intron 35.0%
! TTGCTTAGAGTATTTCCCTT+AGG + chr3_4:48665444-48665463 None:intergenic 35.0%
!! AACTAGAAGTGCTTGTTGAT+GGG - chr3_4:48665547-48665566 Msa0504370:CDS 35.0%
CATAAGATGGTACATGGTAA+GGG + chr3_4:48665680-48665699 None:intergenic 35.0%
ACATAAGATGGTACATGGTA+AGG + chr3_4:48665681-48665700 None:intergenic 35.0%
! AACTCTCTTTTTGCCATTCC+TGG + chr3_4:48665127-48665146 None:intergenic 40.0%
AGAGAGTTAGAGCTGTTTGT+TGG - chr3_4:48665139-48665158 Msa0504370:CDS 40.0%
ACCATTCCACAAGTATGCAT+CGG + chr3_4:48665243-48665262 None:intergenic 40.0%
!! TTCCAGGTCTAAGTGGATTA+AGG - chr3_4:48665278-48665297 Msa0504370:intron 40.0%
TTCCTTAATCCACTTAGACC+TGG + chr3_4:48665283-48665302 None:intergenic 40.0%
!! TGTGTTTGTTATATCAGGGG+AGG - chr3_4:48665418-48665437 Msa0504370:CDS 40.0%
! TTTCTCTTTGCCGCTTCATT+TGG + chr3_4:48665474-48665493 None:intergenic 40.0%
!! CAACTAGAAGTGCTTGTTGA+TGG - chr3_4:48665546-48665565 Msa0504370:CDS 40.0%
!! ACTAGAAGTGCTTGTTGATG+GGG - chr3_4:48665548-48665567 Msa0504370:CDS 40.0%
! ACTTCTTGATTCTGGTTGAG+AGG + chr3_4:48665576-48665595 None:intergenic 40.0%
TTTCGCCAACATATCGTCAT+CGG + chr3_4:48665604-48665623 None:intergenic 40.0%
ACAAGGTTCACTGCAGAAAT+CGG + chr3_4:48665642-48665661 None:intergenic 40.0%
GACTCACATAAGATGGTACA+TGG + chr3_4:48665686-48665705 None:intergenic 40.0%
! AACTCTCTTTTTGCCATTCC+TGG + chr3_4:48665127-48665146 None:intergenic 40.0%
AGAGAGTTAGAGCTGTTTGT+TGG - chr3_4:48665139-48665158 Msa0504370:CDS 40.0%
ACCATTCCACAAGTATGCAT+CGG + chr3_4:48665243-48665262 None:intergenic 40.0%
!! TTCCAGGTCTAAGTGGATTA+AGG - chr3_4:48665278-48665297 Msa0504370:intron 40.0%
TTCCTTAATCCACTTAGACC+TGG + chr3_4:48665283-48665302 None:intergenic 40.0%
!! TGTGTTTGTTATATCAGGGG+AGG - chr3_4:48665418-48665437 Msa0504370:CDS 40.0%
! TTTCTCTTTGCCGCTTCATT+TGG + chr3_4:48665474-48665493 None:intergenic 40.0%
!! CAACTAGAAGTGCTTGTTGA+TGG - chr3_4:48665546-48665565 Msa0504370:CDS 40.0%
!! ACTAGAAGTGCTTGTTGATG+GGG - chr3_4:48665548-48665567 Msa0504370:CDS 40.0%
! ACTTCTTGATTCTGGTTGAG+AGG + chr3_4:48665576-48665595 None:intergenic 40.0%
TTTCGCCAACATATCGTCAT+CGG + chr3_4:48665604-48665623 None:intergenic 40.0%
ACAAGGTTCACTGCAGAAAT+CGG + chr3_4:48665642-48665661 None:intergenic 40.0%
GACTCACATAAGATGGTACA+TGG + chr3_4:48665686-48665705 None:intergenic 40.0%
! AAGAAGCACAGTGTGTGGAA+TGG - chr3_4:48664894-48664913 Msa0504370:CDS 45.0%
CTCGAACAGCGTCCTTAAAT+TGG + chr3_4:48665068-48665087 None:intergenic 45.0%
CTACATCCGATGCATACTTG+TGG - chr3_4:48665234-48665253 Msa0504370:intron 45.0%
TCCGATGCATACTTGTGGAA+TGG - chr3_4:48665239-48665258 Msa0504370:intron 45.0%
TCAAGAGTTCCAGGTCTAAG+TGG - chr3_4:48665271-48665290 Msa0504370:intron 45.0%
TTATATCAGGGGAGGCCTAA+GGG - chr3_4:48665426-48665445 Msa0504370:CDS 45.0%
CGGCAGATACTTCTTGATTC+TGG + chr3_4:48665584-48665603 None:intergenic 45.0%
ATCTGCCGATGACGATATGT+TGG - chr3_4:48665596-48665615 Msa0504370:CDS 45.0%
! AAGAAGCACAGTGTGTGGAA+TGG - chr3_4:48664894-48664913 Msa0504370:CDS 45.0%
CTCGAACAGCGTCCTTAAAT+TGG + chr3_4:48665068-48665087 None:intergenic 45.0%
CTACATCCGATGCATACTTG+TGG - chr3_4:48665234-48665253 Msa0504370:intron 45.0%
TCCGATGCATACTTGTGGAA+TGG - chr3_4:48665239-48665258 Msa0504370:intron 45.0%
TCAAGAGTTCCAGGTCTAAG+TGG - chr3_4:48665271-48665290 Msa0504370:intron 45.0%
TTATATCAGGGGAGGCCTAA+GGG - chr3_4:48665426-48665445 Msa0504370:CDS 45.0%
CGGCAGATACTTCTTGATTC+TGG + chr3_4:48665584-48665603 None:intergenic 45.0%
ATCTGCCGATGACGATATGT+TGG - chr3_4:48665596-48665615 Msa0504370:CDS 45.0%
GTTATATCAGGGGAGGCCTA+AGG - chr3_4:48665425-48665444 Msa0504370:CDS 50.0%
GGTCAGCAGTTGTTGGTACA+AGG + chr3_4:48665659-48665678 None:intergenic 50.0%
TGGTAAGGGTCAGCAGTTGT+TGG + chr3_4:48665666-48665685 None:intergenic 50.0%
GTTATATCAGGGGAGGCCTA+AGG - chr3_4:48665425-48665444 Msa0504370:CDS 50.0%
GGTCAGCAGTTGTTGGTACA+AGG + chr3_4:48665659-48665678 None:intergenic 50.0%
TGGTAAGGGTCAGCAGTTGT+TGG + chr3_4:48665666-48665685 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 48664885 48665740 48664885 ID=Msa0504370;Name=Msa0504370
chr3_4 mRNA 48664885 48665740 48664885 ID=Msa0504370-mRNA-1;Parent=Msa0504370;Name=Msa0504370-mRNA-1;_AED=0.23;_eAED=0.50;_QI=1|0.5|0|1|0.5|0.33|3|0|240
chr3_4 exon 48665530 48665740 48665530 ID=Msa0504370-mRNA-1:exon:10495;Parent=Msa0504370-mRNA-1
chr3_4 exon 48665281 48665484 48665281 ID=Msa0504370-mRNA-1:exon:10494;Parent=Msa0504370-mRNA-1
chr3_4 exon 48664885 48665190 48664885 ID=Msa0504370-mRNA-1:exon:10493;Parent=Msa0504370-mRNA-1
chr3_4 five_prime_UTR 48665740 48665740 48665740 ID=Msa0504370-mRNA-1:five_prime_utr;Parent=Msa0504370-mRNA-1
chr3_4 CDS 48665530 48665739 48665530 ID=Msa0504370-mRNA-1:cds;Parent=Msa0504370-mRNA-1
chr3_4 CDS 48665281 48665484 48665281 ID=Msa0504370-mRNA-1:cds;Parent=Msa0504370-mRNA-1
chr3_4 CDS 48664885 48665190 48664885 ID=Msa0504370-mRNA-1:cds;Parent=Msa0504370-mRNA-1
Gene Sequence

>Msa0504370

ATGTTATTAAGAAGCACAGTGTGTGGAATGGATAGCGAGAAGTTGTCAAATGAATTGGACAATGCATCTAAGGAAGATTCTGAAATGAAGGGTAAGCCAAAATATGATAAGTTCGATGCAGAAGCTGAAATCACCAAAGATTTCAAATTCAGTATCGGAATGGAGTTTACTTCTCGACACCAATTTAAGGACGCTGTTCGAGAGCTCAATAGAGTTAGAGCTGTTTGTTGGCTCAACCAAAAATGTTCATACAATGTTTATGTGAGTATAGCGAAAAAGACTAATACATTTAGGGTGAGGAAACTACATCCGATGCATACTTGTGGAATGGTGTTAAATTTCAAGAGTTCCAGGTCTAAGTGGATTAAGGAAGTCTTTGTTAAACACAATATCAATCAAGAACCTAAAAAGAGGGGGAGGCCTAAGGGAAATACTCTAAGCAATGTTAAACCAAATGAAGCGGCAAAGAGAAAAACAAAACAACGCGATGCATGTTATAAGAAGACAGAAGAATTAGAGCAACAACAACTAGAAGTGCTTGTTGATGGGGAGCCTCTCAACCAGAATCAAGAAGTATCTGCCGATGACGATATGTTGGCGAAAAACACATTTGTTGAGCCGATTTCTGCAGTGAACCTTGTACCAACAACTGCTGACCCTTACCATGTACCATCTTATGTGAGTCTTCATATCTTCAATTTAATACAATTCTTAGTTACT

Protein sequence

>Msa0504370

MLLRSTVCGMDSEKLSNELDNASKEDSEMKGKPKYDKFDAEAEITKDFKFSIGMEFTSRHQFKDAVRELNRVRAVCWLNQKCSYNVYVSIAKKTNTFRVRKLHPMHTCGMVLNFKSSRSKWIKEVFVKHNINQEPKKRGRPKGNTLSNVKPNEAAKRKTKQRDACYKKTEELEQQQLEVLVDGEPLNQNQEVSADDDMLAKNTFVEPISAVNLVPTTADPYHVPSYVSLHIFNLIQFLVT