AlfalfaGEDB | Msa1082500

M. sativa cultivar zhongmu-4 / Msa1082500

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1082500	XP_012570105.1	75.610	41	10	0	1	41	622	662	4.09e-11	67.8

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1082500	A0A1S3E2Y4	75.610	41	10	0	1	41	622	662	1.95e-11	67.8

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000550	Msa1082500	-0.858302	9.046355e-63	-8.615850e-47
Msa0001590	Msa1082500	0.809771	1.604814e-50	-8.615850e-47
Msa0001780	Msa1082500	0.824050	9.959418e-54	-8.615850e-47
Msa0001790	Msa1082500	0.837010	6.688880e-57	-8.615850e-47
Msa0002230	Msa1082500	0.808233	3.424468e-50	-8.615850e-47
Msa0002240	Msa1082500	0.802085	6.630529e-49	-8.615850e-47
Msa0002460	Msa1082500	0.834139	3.561672e-56	-8.615850e-47
Msa0002490	Msa1082500	0.800694	1.278084e-48	-8.615850e-47
Msa0003300	Msa1082500	0.800048	1.730347e-48	-8.615850e-47
Msa0003700	Msa1082500	0.823551	1.303027e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1082500	MtrunA17_Chr8g0368761	65.854	41	14	0	1	41	608	648	1.26e-12	60.8
Msa1082500	MtrunA17_Chr1g0179081	63.415	41	15	0	1	41	596	636	4.14e-12	59.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 20 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAAATTCATAAGATTGG+AGG	0.491683	7_2:-82582494	Msa1082500:CDS
AATATTGCATGCTTTGTAGC+TGG	0.484095	7_2:-82582518	Msa1082500:CDS
AGTCGTTGAAGTAGCAGACG+TGG	0.666126	7_2:-82582444	Msa1082500:CDS
CCAGTTTGTTTGTCCCACCC+CGG	0.527349	7_2:-82581809	Msa1082500:CDS
CCCTAAGAGTTGTCCTCTAC+AGG	0.431074	7_2:+82581844	None:intergenic
CCGGGGTGGGACAAACAAAC+TGG	0.410782	7_2:+82581809	None:intergenic
CCTGTAGAGGACAACTCTTA+GGG	0.537100	7_2:-82581844	Msa1082500:CDS
CGGTTGCTTTAAACCGACTC+CGG	0.476303	7_2:+82581790	None:intergenic
CGTTGAAGTAGCAGACGTGG+AGG	0.685057	7_2:-82582441	Msa1082500:CDS
CTTTAAACCGACTCCGGGGT+GGG	0.547172	7_2:+82581796	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GGCAAACAAATTCATAAGAT+TGG	-	chr7_2:82581823-82581842	Msa1082500:CDS	30.0%
GACATCAAACTGTATCTTAT+CGG	-	chr7_2:82582025-82582044	Msa1082500:intron	30.0%
ACATCAAACTGTATCTTATC+GGG	-	chr7_2:82582026-82582045	Msa1082500:intron	30.0%
TACAAGCTTGATCCAAAAAT+TGG	-	chr7_2:82582153-82582172	Msa1082500:intron	30.0%
CCAAAAATTGGTACATAACT+TGG	-	chr7_2:82582165-82582184	Msa1082500:intron	30.0%
GATATCAAACGAAATCTTAC+AGG	+	chr7_2:82582208-82582227	None:intergenic	30.0%
TGCAAACAACTTCATAAGAT+TGG	+	chr7_2:82582400-82582419	None:intergenic	30.0%
GGCAAACAAATTCATAAGAT+TGG	-	chr7_2:82581823-82581842	Msa1082500:CDS	30.0%
GACATCAAACTGTATCTTAT+CGG	-	chr7_2:82582025-82582044	Msa1082500:intron	30.0%
ACATCAAACTGTATCTTATC+GGG	-	chr7_2:82582026-82582045	Msa1082500:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_2	gene	82581781	82582561	82581781	ID=Msa1082500;Name=Msa1082500
chr7_2	mRNA	82581781	82582561	82581781	ID=Msa1082500-mRNA-1;Parent=Msa1082500;Name=Msa1082500-mRNA-1;_AED=0.45;_eAED=1.00;_QI=0\|0\|0\|1\|0\|0\|2\|0\|70
chr7_2	exon	82582435	82582561	82582435	ID=Msa1082500-mRNA-1:exon:21446;Parent=Msa1082500-mRNA-1
chr7_2	exon	82581781	82581866	82581781	ID=Msa1082500-mRNA-1:exon:21445;Parent=Msa1082500-mRNA-1
chr7_2	CDS	82582435	82582561	82582435	ID=Msa1082500-mRNA-1:cds;Parent=Msa1082500-mRNA-1
chr7_2	CDS	82581781	82581866	82581781	ID=Msa1082500-mRNA-1:cds;Parent=Msa1082500-mRNA-1

Gene Sequence	>Msa1082500 GGCGACACAAAGTTCTTAAAGAATATTGCATGCTTTGTAGCTGGCAAACAAATTCATAAGATTGGAGGTTTGAGAGATAATCTCGTTCCTTCTGTAGTCGTTGAAGTAGCAGACGTGGAGGAATCTGCCTGTAGAGGACAACTCTTAGGGAGATACAATGTTCCAGTTTGTTTGTCCCACCCCGGAGTCGGTTTAAAGCAACCGAGAGCTTAG
Protein sequence	>Msa1082500 GDTKFLKNIACFVAGKQIHKIGGLRDNLVPSVVVEVADVEESACRGQLLGRYNVPVCLSHPGVGLKQPRA*