AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0504800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0504800 MtrunA17_Chr3g0105411 96.429 112 4 0 1 112 1 112 1.20e-74 216
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0504800 AT3G26360.2 55.752 113 37 3 1 112 1 101 6.34e-34 113
Msa0504800 AT3G26360.1 55.752 113 37 3 1 112 1 101 6.34e-34 113

Find 55 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCTACGCTCCAAGCTCTTT+CGG 0.238989 3_4:-49865137 None:intergenic
TACTCTTTCAAATTGTTTAC+AGG 0.254678 3_4:+49866606 Msa0504800:three_prime_UTR
ATCAAGTGTTATTGATAATA+AGG 0.266857 3_4:-49866917 None:intergenic
CCTACGCTCCAAGCTCTTTC+GGG 0.273352 3_4:-49865136 None:intergenic
CCTGACTTTCTTGATCATAA+TGG 0.283713 3_4:-49865193 None:intergenic
TGGAATTGAGTTCAGTGTTC+AGG 0.318324 3_4:-49863457 None:intergenic
AAACCATAGCATAGTTATAA+AGG 0.356410 3_4:-49866750 None:intergenic
TGATGATGCTGAGCATTAAA+AGG 0.365982 3_4:-49864946 None:intergenic
TCAATGTCAATGCCCCTTCC+AGG 0.380539 3_4:-49865023 None:intergenic
AAAGGGTTGGTGTAAGTTGC+AGG 0.395496 3_4:+49866543 Msa0504800:three_prime_UTR
TGAGTTCATATCTTGTGAAA+TGG 0.424620 3_4:-49864872 None:intergenic
ATACGAGTGCAAGTCTATAA+TGG 0.437158 3_4:+49864997 Msa0504800:CDS
GGAATTGAGTTCAGTGTTCA+GGG 0.453089 3_4:-49863456 None:intergenic
ATCTAGCTTATTCAGACAGT+CGG 0.453668 3_4:+49864909 Msa0504800:CDS
GAGTCATATACGTGATATCA+TGG 0.480569 3_4:-49866866 None:intergenic
ATCAACGATATGCTATATCA+TGG 0.485249 3_4:-49866802 None:intergenic
CAGCAGCTTCAGCCATGCAG+AGG 0.487027 3_4:+49864973 Msa0504800:CDS
CCATTATGATCAAGAAAGTC+AGG 0.495345 3_4:+49865193 Msa0504800:CDS
TCTAGCTTATTCAGACAGTC+GGG 0.503323 3_4:+49864910 Msa0504800:CDS
AACTCCGGCGGCGTTCTCAA+TGG 0.504097 3_4:-49863477 None:intergenic
TCACCTTTATAACTATGCTA+TGG 0.506706 3_4:+49866747 Msa0504800:three_prime_UTR
AGGTGAGTAACATCATAACT+TGG 0.514797 3_4:-49866730 None:intergenic
GGTGAGTAACATCATAACTT+GGG 0.518640 3_4:-49866729 None:intergenic
TTACAGGGTAATATGTACTT+TGG 0.524598 3_4:+49866622 Msa0504800:three_prime_UTR
GTCTATAATGGAAACCTGGA+AGG 0.531937 3_4:+49865009 Msa0504800:CDS
GTGTTATTGATAATAAGGAC+AGG 0.539770 3_4:-49866912 None:intergenic
CCCGAAAGAGCTTGGAGCGT+AGG 0.558383 3_4:+49865136 Msa0504800:CDS
GAAAGAGTATGCTTCAACTG+CGG 0.559803 3_4:-49866592 None:intergenic
TGCACTCGTATGCCTCTGCA+TGG 0.562028 3_4:-49864985 None:intergenic
GGAGCGTAGGCTTCGATCAG+AGG 0.563295 3_4:+49865149 Msa0504800:CDS
ACTAGTAAGACAAATAAAGT+AGG 0.566151 3_4:+49866689 Msa0504800:three_prime_UTR
AATTCCATTGAGAACGCCGC+CGG 0.576328 3_4:+49863473 Msa0504800:five_prime_UTR
GATCGAGAGGATGATAAAGA+AGG 0.576761 3_4:+49865071 Msa0504800:CDS
GCAAGTCTATAATGGAAACC+TGG 0.578047 3_4:+49865005 Msa0504800:CDS
AACAGCGTAAAACTTCGTCC+AGG 0.582702 3_4:+49863517 Msa0504800:five_prime_UTR
TATGCTATATCATGGATATG+TGG 0.583388 3_4:-49866794 None:intergenic
ATGATATCACGTATATGACT+CGG 0.589197 3_4:+49866868 Msa0504800:three_prime_UTR
ACTCTTTCAAATTGTTTACA+GGG 0.589498 3_4:+49866607 Msa0504800:three_prime_UTR
CAGCGTAAGATGACATCAAG+TGG 0.589882 3_4:+49865048 Msa0504800:CDS
TTCGATCAGAGGATCTTGCT+AGG 0.598839 3_4:+49865160 Msa0504800:CDS
TGGTGGCCGACGAGAAACTC+CGG 0.599773 3_4:-49863492 None:intergenic
TACGTGATATCATGGATACA+TGG 0.605332 3_4:-49866858 None:intergenic
TCTATAATGGAAACCTGGAA+GGG 0.608317 3_4:+49865010 Msa0504800:CDS
CTATAATGGAAACCTGGAAG+GGG 0.613741 3_4:+49865011 Msa0504800:CDS
TGATATCACGTATATGACTC+GGG 0.614927 3_4:+49866869 Msa0504800:three_prime_UTR
TGACATCAAGTGGGATCGAG+AGG 0.620298 3_4:+49865058 Msa0504800:CDS
GTGAGTAACATCATAACTTG+GGG 0.629595 3_4:-49866728 None:intergenic
CATGCAATATCGAGGATGCA+CGG 0.631477 3_4:-49866826 None:intergenic
AGCGTAAGATGACATCAAGT+GGG 0.633900 3_4:+49865049 Msa0504800:CDS
ATCAAAGACATGCAATATCG+AGG 0.642935 3_4:-49866834 None:intergenic
ACGCCGCCGGAGTTTCTCGT+CGG 0.651685 3_4:+49863486 Msa0504800:five_prime_UTR
AGCTTATTCAGACAGTCGGG+TGG 0.680571 3_4:+49864913 Msa0504800:CDS
AGTGTTCAGGGAGAACGATG+CGG 0.682556 3_4:-49863444 None:intergenic
TGTTATTGATAATAAGGACA+GGG 0.695345 3_4:-49866911 None:intergenic
TGGCCGACGAGAAACTCCGG+CGG 0.727297 3_4:-49863489 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTAAAAATCACATTTTTTTT+AGG - chr3_4:49864005-49864024 None:intergenic 15.0%
!!! ATTTTATTATGCTAATTACA+TGG - chr3_4:49864567-49864586 None:intergenic 15.0%
!! AACATTATTTAGATACTATA+TGG - chr3_4:49864629-49864648 None:intergenic 15.0%
!!! TAAAAATTGGTATTTTGTAT+AGG - chr3_4:49865329-49865348 None:intergenic 15.0%
!!! AATATATTTTAAAGAATCAC+TGG - chr3_4:49865377-49865396 None:intergenic 15.0%
!!! CAATATTACTTTATTTCATA+CGG + chr3_4:49865450-49865469 Msa0504800:intron 15.0%
!! TTATATTGTTTACTTACAAT+TGG + chr3_4:49865797-49865816 Msa0504800:intron 15.0%
!! TTACAAATTATTAACTGTGT+CGG - chr3_4:49863823-49863842 None:intergenic 20.0%
!! TTGTAAAAATAGCATAATTC+AGG + chr3_4:49863837-49863856 Msa0504800:intron 20.0%
!! TGTAAAAATAGCATAATTCA+GGG + chr3_4:49863838-49863857 Msa0504800:intron 20.0%
!! AAGTTATCATAATCTATGTT+TGG - chr3_4:49864069-49864088 None:intergenic 20.0%
!! TTGTTCTATTGATAAAGAAA+TGG + chr3_4:49864404-49864423 Msa0504800:intron 20.0%
!! TGTTCTATTGATAAAGAAAT+GGG + chr3_4:49864405-49864424 Msa0504800:intron 20.0%
!! AAGTTGTTTAGAAAATACTA+GGG - chr3_4:49864661-49864680 None:intergenic 20.0%
!! TAAGTTGTTTAGAAAATACT+AGG - chr3_4:49864662-49864681 None:intergenic 20.0%
!!! TATAATTCAGTCTTTGAAAT+AGG - chr3_4:49865228-49865247 None:intergenic 20.0%
!! TTATTTGGCATATAATATCT+TGG + chr3_4:49865554-49865573 Msa0504800:intron 20.0%
!!! ATATAATATCTTGGTGTTTT+AGG + chr3_4:49865563-49865582 Msa0504800:intron 20.0%
!!! TTTTAATTGAAATGAGTACA+TGG + chr3_4:49866207-49866226 Msa0504800:intron 20.0%
!!! AGTTATTTCAAAAGAATTGA+AGG + chr3_4:49866235-49866254 Msa0504800:intron 20.0%
!!! GTTATTTCAAAAGAATTGAA+GGG + chr3_4:49866236-49866255 Msa0504800:intron 20.0%
!! ATGAAGTTTATCGTAATTAA+TGG - chr3_4:49866350-49866369 None:intergenic 20.0%
!! AATTACGATAAACTTCATAT+TGG + chr3_4:49866352-49866371 Msa0504800:intron 20.0%
!! TGAAGGTAAAAATATTACAA+AGG - chr3_4:49866404-49866423 None:intergenic 20.0%
!! GAGAAACAAAAAAATAGTTA+GGG - chr3_4:49866437-49866456 None:intergenic 20.0%
!! AGAGAAACAAAAAAATAGTT+AGG - chr3_4:49866438-49866457 None:intergenic 20.0%
!! TTATTCTATTGAAATATGAC+AGG + chr3_4:49866486-49866505 Msa0504800:intron 20.0%
!! TATTCTATTGAAATATGACA+GGG + chr3_4:49866487-49866506 Msa0504800:intron 20.0%
!!! ATCAAGTGTTATTGATAATA+AGG - chr3_4:49866920-49866939 None:intergenic 20.0%
! TGATAACTTAAACTCACTTT+AGG + chr3_4:49864080-49864099 Msa0504800:intron 25.0%
! GATAACTTAAACTCACTTTA+GGG + chr3_4:49864081-49864100 Msa0504800:intron 25.0%
! AAATGAAATCAACATCGTAA+TGG + chr3_4:49864252-49864271 Msa0504800:intron 25.0%
! AAACTAGAATGTAATCCATT+GGG - chr3_4:49864279-49864298 None:intergenic 25.0%
! TAAACTAGAATGTAATCCAT+TGG - chr3_4:49864280-49864299 None:intergenic 25.0%
! TTAAGCAGTCATACAAAAAA+CGG - chr3_4:49864327-49864346 None:intergenic 25.0%
! ATTGATAAAGAAATGGGTAA+AGG + chr3_4:49864411-49864430 Msa0504800:intron 25.0%
! TTGATAAAGAAATGGGTAAA+GGG + chr3_4:49864412-49864431 Msa0504800:intron 25.0%
! ATACTTTAAATGACTTTCGA+CGG + chr3_4:49864496-49864515 Msa0504800:intron 25.0%
! AGCATAATAAAATTTGACTG+AGG + chr3_4:49864574-49864593 Msa0504800:intron 25.0%
!! CACTAAATTAGAATTTTCCA+AGG - chr3_4:49864697-49864716 None:intergenic 25.0%
!!! GTTTTAATTGTTCTCAGAAA+AGG + chr3_4:49864829-49864848 Msa0504800:intron 25.0%
!!! TTTTTAGAGCATAGAATAGT+AGG + chr3_4:49865249-49865268 Msa0504800:intron 25.0%
! AGCTTAAATGTTTCTTATCA+AGG - chr3_4:49865277-49865296 None:intergenic 25.0%
!! GAATTTCTGAGAAACAATAT+AGG + chr3_4:49865585-49865604 Msa0504800:intron 25.0%
! GTTCAATAGTTAGATGTAAA+GGG - chr3_4:49865699-49865718 None:intergenic 25.0%
! AGTTCAATAGTTAGATGTAA+AGG - chr3_4:49865700-49865719 None:intergenic 25.0%
! CATATTAGATGGTATATGTT+CGG - chr3_4:49865774-49865793 None:intergenic 25.0%
! AACATATACCATCTAATATG+AGG + chr3_4:49865774-49865793 Msa0504800:intron 25.0%
! CAATATAACCTCATATTAGA+TGG - chr3_4:49865785-49865804 None:intergenic 25.0%
!! AATAATATTTCCAGTCTTGT+TGG + chr3_4:49865844-49865863 Msa0504800:intron 25.0%
! CATTCATCTTAACAAAAGTT+TGG + chr3_4:49865869-49865888 Msa0504800:intron 25.0%
! AATGGAAACAAGAAAAAATG+TGG - chr3_4:49866305-49866324 None:intergenic 25.0%
!!! TTTTCTTGTTTCCATTTTTG+TGG + chr3_4:49866309-49866328 Msa0504800:intron 25.0%
! AAACTTCATATTGGATTGAA+TGG + chr3_4:49866361-49866380 Msa0504800:intron 25.0%
! AACTTCATATTGGATTGAAT+GGG + chr3_4:49866362-49866381 Msa0504800:intron 25.0%
! AAAAAAATAGTTAGGGCAAA+AGG - chr3_4:49866430-49866449 None:intergenic 25.0%
! ATTCTATTGAAATATGACAG+GGG + chr3_4:49866488-49866507 Msa0504800:intron 25.0%
! TACTCTTTCAAATTGTTTAC+AGG + chr3_4:49866606-49866625 Msa0504800:three_prime_UTR 25.0%
! ACTCTTTCAAATTGTTTACA+GGG + chr3_4:49866607-49866626 Msa0504800:three_prime_UTR 25.0%
! ACTAGTAAGACAAATAAAGT+AGG + chr3_4:49866689-49866708 Msa0504800:three_prime_UTR 25.0%
! AAACCATAGCATAGTTATAA+AGG - chr3_4:49866753-49866772 None:intergenic 25.0%
!! TGTTATTGATAATAAGGACA+GGG - chr3_4:49866914-49866933 None:intergenic 25.0%
!! TGCATTTCGTGTTTGTTATT+CGG + chr3_4:49863719-49863738 Msa0504800:intron 30.0%
! TTTAGGGTGTGTTTAATTAG+TGG + chr3_4:49864097-49864116 Msa0504800:intron 30.0%
TTAGGGTGTGTTTAATTAGT+GGG + chr3_4:49864098-49864117 Msa0504800:intron 30.0%
! TAGACATAATGGAATGCTAA+TGG + chr3_4:49864185-49864204 Msa0504800:intron 30.0%
TGATAAAGAAATGGGTAAAG+GGG + chr3_4:49864413-49864432 Msa0504800:intron 30.0%
! GAAATCGTTGGTTAAGAATA+AGG + chr3_4:49864471-49864490 Msa0504800:intron 30.0%
AGAAAATACTAGGGTAGAAT+TGG - chr3_4:49864652-49864671 None:intergenic 30.0%
ACTTACTACAAACAATTCCT+TGG + chr3_4:49864677-49864696 Msa0504800:intron 30.0%
! ATATTTGACCTTCCTCTTTT+TGG + chr3_4:49864735-49864754 Msa0504800:intron 30.0%
AAAAACATGCAACCAAAAAG+AGG - chr3_4:49864750-49864769 None:intergenic 30.0%
TGAGTTCATATCTTGTGAAA+TGG - chr3_4:49864875-49864894 None:intergenic 30.0%
CTACAATGAGGGATAAAAAT+TGG - chr3_4:49865342-49865361 None:intergenic 30.0%
TTCCCAATACATCAGTTATT+TGG + chr3_4:49865539-49865558 Msa0504800:intron 30.0%
TGCCAAATAACTGATGTATT+GGG - chr3_4:49865544-49865563 None:intergenic 30.0%
ATGCCAAATAACTGATGTAT+TGG - chr3_4:49865545-49865564 None:intergenic 30.0%
! GGTTGACCTATAGTTTTTAT+AGG + chr3_4:49865670-49865689 Msa0504800:intron 30.0%
GGGATACCTATAAAAACTAT+AGG - chr3_4:49865679-49865698 None:intergenic 30.0%
TTTACTTACAATTGGAGCTA+TGG + chr3_4:49865805-49865824 Msa0504800:intron 30.0%
!! TCATTCAATTTCTAGCTATC+TGG + chr3_4:49866263-49866282 Msa0504800:intron 30.0%
GAAACAAGAAAAAATGTGGT+TGG - chr3_4:49866301-49866320 None:intergenic 30.0%
AAATAGAGACTATGTCAGTA+CGG - chr3_4:49866467-49866486 None:intergenic 30.0%
CACTGCTATTGAGAAAAAAA+GGG + chr3_4:49866526-49866545 Msa0504800:three_prime_UTR 30.0%
TTACAGGGTAATATGTACTT+TGG + chr3_4:49866622-49866641 Msa0504800:three_prime_UTR 30.0%
TCACCTTTATAACTATGCTA+TGG + chr3_4:49866747-49866766 Msa0504800:three_prime_UTR 30.0%
TATGCTATATCATGGATATG+TGG - chr3_4:49866797-49866816 None:intergenic 30.0%
ATCAACGATATGCTATATCA+TGG - chr3_4:49866805-49866824 None:intergenic 30.0%
ATGATATCACGTATATGACT+CGG + chr3_4:49866868-49866887 Msa0504800:three_prime_UTR 30.0%
! GTGTTATTGATAATAAGGAC+AGG - chr3_4:49866915-49866934 None:intergenic 30.0%
! CAATTTTAAGCGCGCAATAA+CGG - chr3_4:49863628-49863647 None:intergenic 35.0%
!! TGCGCGCTTAAAATTGATTT+TGG + chr3_4:49863632-49863651 Msa0504800:intron 35.0%
!! GCATTTCGTGTTTGTTATTC+GGG + chr3_4:49863720-49863739 Msa0504800:intron 35.0%
TTCAGGGTAATTACAAGTGT+CGG + chr3_4:49863854-49863873 Msa0504800:intron 35.0%
CTTCATAGGTCATTGTGATA+CGG + chr3_4:49863940-49863959 Msa0504800:intron 35.0%
TAGGGTGTGTTTAATTAGTG+GGG + chr3_4:49864099-49864118 Msa0504800:intron 35.0%
TTAGTGGGGATGTAAATATG+AGG + chr3_4:49864113-49864132 Msa0504800:intron 35.0%
AAAACATTTGTGTGTTGCCT+CGG + chr3_4:49864153-49864172 Msa0504800:intron 35.0%
GGTCGAAAGAATAGACATAA+TGG + chr3_4:49864174-49864193 Msa0504800:intron 35.0%
GATAAAGAAATGGGTAAAGG+GGG + chr3_4:49864414-49864433 Msa0504800:intron 35.0%
ATAAAGAAATGGGTAAAGGG+GGG + chr3_4:49864415-49864434 Msa0504800:intron 35.0%
! AAAAGCCTTATGAGATCACT+AGG + chr3_4:49864446-49864465 Msa0504800:intron 35.0%
TTCTACCTAGTGATCTCATA+AGG - chr3_4:49864454-49864473 None:intergenic 35.0%
AGGTCAAATATCTTTCTCTG+TGG - chr3_4:49864726-49864745 None:intergenic 35.0%
TCTACAACAGAAGCAAAGAT+CGG - chr3_4:49864790-49864809 None:intergenic 35.0%
! ATCTAGCTTATTCAGACAGT+CGG + chr3_4:49864909-49864928 Msa0504800:CDS 35.0%
! TGATGATGCTGAGCATTAAA+AGG - chr3_4:49864949-49864968 None:intergenic 35.0%
ATACGAGTGCAAGTCTATAA+TGG + chr3_4:49864997-49865016 Msa0504800:CDS 35.0%
! TCTATAATGGAAACCTGGAA+GGG + chr3_4:49865010-49865029 Msa0504800:CDS 35.0%
! GCTTCTCAGAGTTTTTGATA+TGG - chr3_4:49865107-49865126 None:intergenic 35.0%
CCTGACTTTCTTGATCATAA+TGG - chr3_4:49865196-49865215 None:intergenic 35.0%
CCATTATGATCAAGAAAGTC+AGG + chr3_4:49865193-49865212 Msa0504800:CDS 35.0%
GCTATTATCATCAACGAATC+AGG + chr3_4:49865294-49865313 Msa0504800:intron 35.0%
!! TGCATAGTTTGCTACAATGA+GGG - chr3_4:49865353-49865372 None:intergenic 35.0%
!! ATGCATAGTTTGCTACAATG+AGG - chr3_4:49865354-49865373 None:intergenic 35.0%
AAAAAATTCCCACAACTGCA+GGG - chr3_4:49865424-49865443 None:intergenic 35.0%
AATTCCCTCACAATTAACTC+AGG + chr3_4:49865480-49865499 Msa0504800:intron 35.0%
TTGTCCTGAGTTAATTGTGA+GGG - chr3_4:49865487-49865506 None:intergenic 35.0%
ATTGTCCTGAGTTAATTGTG+AGG - chr3_4:49865488-49865507 None:intergenic 35.0%
AAAAAGACACTGGATTCACA+TGG - chr3_4:49865521-49865540 None:intergenic 35.0%
ATGTATTGGGAAAAAGACAC+TGG - chr3_4:49865531-49865550 None:intergenic 35.0%
AACTATAGGTCAACCCAATA+TGG - chr3_4:49865665-49865684 None:intergenic 35.0%
!!! AATTTTTAGCAGTACCCCTA+TGG + chr3_4:49865735-49865754 Msa0504800:intron 35.0%
TGTTAACTTAGCTGCACTAT+CGG + chr3_4:49865970-49865989 Msa0504800:intron 35.0%
ACAAGGTTTCACATAGTGTA+TGG - chr3_4:49866137-49866156 None:intergenic 35.0%
GAAACCTTGTTAGTTATGCA+TGG + chr3_4:49866147-49866166 Msa0504800:intron 35.0%
!! TCTTGTTTCCATTTTTGTGG+AGG + chr3_4:49866312-49866331 Msa0504800:intron 35.0%
GCACTGCTATTGAGAAAAAA+AGG + chr3_4:49866525-49866544 Msa0504800:three_prime_UTR 35.0%
GCTATTGAGAAAAAAAGGGT+TGG + chr3_4:49866530-49866549 Msa0504800:three_prime_UTR 35.0%
! AGGCTATGCAGTAATTTTGT+AGG + chr3_4:49866563-49866582 Msa0504800:three_prime_UTR 35.0%
GTGAGTAACATCATAACTTG+GGG - chr3_4:49866731-49866750 None:intergenic 35.0%
GGTGAGTAACATCATAACTT+GGG - chr3_4:49866732-49866751 None:intergenic 35.0%
AGGTGAGTAACATCATAACT+TGG - chr3_4:49866733-49866752 None:intergenic 35.0%
ATCAAAGACATGCAATATCG+AGG - chr3_4:49866837-49866856 None:intergenic 35.0%
TACGTGATATCATGGATACA+TGG - chr3_4:49866861-49866880 None:intergenic 35.0%
GAGTCATATACGTGATATCA+TGG - chr3_4:49866869-49866888 None:intergenic 35.0%
TGATATCACGTATATGACTC+GGG + chr3_4:49866869-49866888 Msa0504800:three_prime_UTR 35.0%
GGAATTGAGTTCAGTGTTCA+GGG - chr3_4:49863459-49863478 None:intergenic 40.0%
TGGAATTGAGTTCAGTGTTC+AGG - chr3_4:49863460-49863479 None:intergenic 40.0%
! AAGTTTTACGCTGTTCTTGG+TGG - chr3_4:49863512-49863531 None:intergenic 40.0%
! ACGAAGTTTTACGCTGTTCT+TGG - chr3_4:49863515-49863534 None:intergenic 40.0%
GGAAGGAAAAGAGAAAAACC+TGG - chr3_4:49863538-49863557 None:intergenic 40.0%
GAGTCATTGTCTATGAAGCA+CGG + chr3_4:49863763-49863782 Msa0504800:intron 40.0%
TTGTCTATGAAGCACGGATA+CGG + chr3_4:49863769-49863788 Msa0504800:intron 40.0%
!! GTAATTACAAGTGTCGGTGT+CGG + chr3_4:49863860-49863879 Msa0504800:intron 40.0%
TATGTCTATTCTTTCGACCG+AGG - chr3_4:49864173-49864192 None:intergenic 40.0%
GATCACTAGGTAGAAATCGT+TGG + chr3_4:49864459-49864478 Msa0504800:intron 40.0%
ACATGCAACCAAAAAGAGGA+AGG - chr3_4:49864746-49864765 None:intergenic 40.0%
! TCTAGCTTATTCAGACAGTC+GGG + chr3_4:49864910-49864929 Msa0504800:CDS 40.0%
! GCAAGTCTATAATGGAAACC+TGG + chr3_4:49865005-49865024 Msa0504800:CDS 40.0%
! GTCTATAATGGAAACCTGGA+AGG + chr3_4:49865009-49865028 Msa0504800:CDS 40.0%
! CTATAATGGAAACCTGGAAG+GGG + chr3_4:49865011-49865030 Msa0504800:CDS 40.0%
AGCGTAAGATGACATCAAGT+GGG + chr3_4:49865049-49865068 Msa0504800:CDS 40.0%
GATCGAGAGGATGATAAAGA+AGG + chr3_4:49865071-49865090 Msa0504800:CDS 40.0%
GAAAAAATTCCCACAACTGC+AGG - chr3_4:49865425-49865444 None:intergenic 40.0%
CTTGTCACCTTGACCATATT+GGG + chr3_4:49865649-49865668 Msa0504800:intron 40.0%
GATGAATGATCCAACAAGAC+TGG - chr3_4:49865857-49865876 None:intergenic 40.0%
GACTCCATGCATAACTAACA+AGG - chr3_4:49866154-49866173 None:intergenic 40.0%
!! TTTCCATTTTTGTGGAGGCT+TGG + chr3_4:49866317-49866336 Msa0504800:intron 40.0%
GTTAGGGCAAAAGGAAATGA+AGG - chr3_4:49866421-49866440 None:intergenic 40.0%
! GAAAGAGTATGCTTCAACTG+CGG - chr3_4:49866595-49866614 None:intergenic 40.0%
AACAGCGTAAAACTTCGTCC+AGG + chr3_4:49863517-49863536 Msa0504800:five_prime_UTR 45.0%
TCAGTATCATCCGACTATCG+CGG - chr3_4:49863584-49863603 None:intergenic 45.0%
! GCAATAACGGAATCAAGGTG+CGG - chr3_4:49863615-49863634 None:intergenic 45.0%
! CGGGTAGTTTGTCAGAATCA+CGG + chr3_4:49863739-49863758 Msa0504800:intron 45.0%
TATGAAGCACAAACCCTCCT+CGG - chr3_4:49863927-49863946 None:intergenic 45.0%
!! CAACATCGTAATGGACCCAA+TGG + chr3_4:49864261-49864280 Msa0504800:intron 45.0%
CAGCGTAAGATGACATCAAG+TGG + chr3_4:49865048-49865067 Msa0504800:CDS 45.0%
!!! TGTTTTAGCCCGAAAGAGCT+TGG + chr3_4:49865128-49865147 Msa0504800:CDS 45.0%
TTCGATCAGAGGATCTTGCT+AGG + chr3_4:49865160-49865179 Msa0504800:CDS 45.0%
! CACAACTGCAGGGTATTCTT+GGG - chr3_4:49865414-49865433 None:intergenic 45.0%
CAAGAATACCCTGCAGTTGT+GGG + chr3_4:49865413-49865432 Msa0504800:intron 45.0%
CCAATATGGTCAAGGTGACA+AGG - chr3_4:49865651-49865670 None:intergenic 45.0%
CCTTGTCACCTTGACCATAT+TGG + chr3_4:49865648-49865667 Msa0504800:intron 45.0%
AGGTCAACCCAATATGGTCA+AGG - chr3_4:49865659-49865678 None:intergenic 45.0%
TCTTCTGAGTCAGAGTCTTC+AGG - chr3_4:49866057-49866076 None:intergenic 45.0%
!! AAAGGGTTGGTGTAAGTTGC+AGG + chr3_4:49866543-49866562 Msa0504800:three_prime_UTR 45.0%
CATGCAATATCGAGGATGCA+CGG - chr3_4:49866829-49866848 None:intergenic 45.0%
AGTGTTCAGGGAGAACGATG+CGG - chr3_4:49863447-49863466 None:intergenic 50.0%
AATTCCATTGAGAACGCCGC+CGG + chr3_4:49863473-49863492 Msa0504800:five_prime_UTR 50.0%
CAATTGGCGCTACAGGAGAA+GGG - chr3_4:49863559-49863578 None:intergenic 50.0%
! AGCGCGCAATAACGGAATCA+AGG - chr3_4:49863620-49863639 None:intergenic 50.0%
ATACGGACACAGACACGACA+CGG + chr3_4:49863786-49863805 Msa0504800:intron 50.0%
!! ACAAGTGTCGGTGTCGGTAT+TGG + chr3_4:49863866-49863885 Msa0504800:intron 50.0%
GAGGAGGGTTTGTGCTTCAT+AGG + chr3_4:49863926-49863945 Msa0504800:intron 50.0%
AGCTTATTCAGACAGTCGGG+TGG + chr3_4:49864913-49864932 Msa0504800:CDS 50.0%
TCAATGTCAATGCCCCTTCC+AGG - chr3_4:49865026-49865045 None:intergenic 50.0%
TGACATCAAGTGGGATCGAG+AGG + chr3_4:49865058-49865077 Msa0504800:CDS 50.0%
! CCACAACTGCAGGGTATTCT+TGG - chr3_4:49865415-49865434 None:intergenic 50.0%
CCAAGAATACCCTGCAGTTG+TGG + chr3_4:49865412-49865431 Msa0504800:intron 50.0%
TAGCAGTACCCCTATGGCAA+TGG + chr3_4:49865741-49865760 Msa0504800:intron 50.0%
AGCAGTACCCCTATGGCAAT+GGG + chr3_4:49865742-49865761 Msa0504800:intron 50.0%
GACAACACCCATTGCCATAG+GGG - chr3_4:49865752-49865771 None:intergenic 50.0%
GGACAACACCCATTGCCATA+GGG - chr3_4:49865753-49865772 None:intergenic 50.0%
CTGCCAAGCCTCCACAAAAA+TGG - chr3_4:49866323-49866342 None:intergenic 50.0%
! CATTTTTGTGGAGGCTTGGC+AGG + chr3_4:49866321-49866340 Msa0504800:intron 50.0%
GCAATTGGCGCTACAGGAGA+AGG - chr3_4:49863560-49863579 None:intergenic 55.0%
AACGGAATCAAGGTGCGGAG+TGG - chr3_4:49863610-49863629 None:intergenic 55.0%
ACACGACATGTGTCCGACAC+CGG + chr3_4:49863889-49863908 Msa0504800:intron 55.0%
GCACAAACCCTCCTCGGATT+AGG - chr3_4:49863921-49863940 None:intergenic 55.0%
TGCACTCGTATGCCTCTGCA+TGG - chr3_4:49864988-49865007 None:intergenic 55.0%
CCTACGCTCCAAGCTCTTTC+GGG - chr3_4:49865139-49865158 None:intergenic 55.0%
GCCTACGCTCCAAGCTCTTT+CGG - chr3_4:49865140-49865159 None:intergenic 55.0%
CGGACAACACCCATTGCCAT+AGG - chr3_4:49865754-49865773 None:intergenic 55.0%
AACTCCGGCGGCGTTCTCAA+TGG - chr3_4:49863480-49863499 None:intergenic 60.0%
TGGTGGCCGACGAGAAACTC+CGG - chr3_4:49863495-49863514 None:intergenic 60.0%
TGGCGCTACAGGAGAAGGGA+AGG - chr3_4:49863555-49863574 None:intergenic 60.0%
GCCAATTGCGCCGCGATAGT+CGG + chr3_4:49863571-49863590 Msa0504800:intron 60.0%
TCCGACTATCGCGGCGCAAT+TGG - chr3_4:49863575-49863594 None:intergenic 60.0%
CACGACATGTGTCCGACACC+GGG + chr3_4:49863890-49863909 Msa0504800:intron 60.0%
GATTAGGCGTGTCCCGGTGT+CGG - chr3_4:49863905-49863924 None:intergenic 60.0%
! TCCTCGGATTAGGCGTGTCC+CGG - chr3_4:49863911-49863930 None:intergenic 60.0%
GGACACGCCTAATCCGAGGA+GGG + chr3_4:49863911-49863930 Msa0504800:intron 60.0%
CAGCAGCTTCAGCCATGCAG+AGG + chr3_4:49864973-49864992 Msa0504800:CDS 60.0%
CCCGAAAGAGCTTGGAGCGT+AGG + chr3_4:49865136-49865155 Msa0504800:CDS 60.0%
GGAGCGTAGGCTTCGATCAG+AGG + chr3_4:49865149-49865168 Msa0504800:CDS 60.0%
ACGCCGCCGGAGTTTCTCGT+CGG + chr3_4:49863486-49863505 Msa0504800:five_prime_UTR 65.0%
TGGCCGACGAGAAACTCCGG+CGG - chr3_4:49863492-49863511 None:intergenic 65.0%
ACCGGGACACGCCTAATCCG+AGG + chr3_4:49863907-49863926 Msa0504800:intron 65.0%
GGGACACGCCTAATCCGAGG+AGG + chr3_4:49863910-49863929 Msa0504800:intron 65.0%
CGCGGCGCAATTGGCGCTAC+AGG - chr3_4:49863566-49863585 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 49863437 49867017 49863437 ID=Msa0504800;Name=Msa0504800
chr3_4 mRNA 49863437 49867017 49863437 ID=Msa0504800-mRNA-1;Parent=Msa0504800;Name=Msa0504800-mRNA-1;_AED=0.05;_eAED=0.05;_QI=142|1|1|1|0.5|0.66|3|500|112
chr3_4 exon 49863437 49863538 49863437 ID=Msa0504800-mRNA-1:exon:10716;Parent=Msa0504800-mRNA-1
chr3_4 exon 49864846 49865214 49864846 ID=Msa0504800-mRNA-1:exon:10717;Parent=Msa0504800-mRNA-1
chr3_4 exon 49866508 49867017 49866508 ID=Msa0504800-mRNA-1:exon:10718;Parent=Msa0504800-mRNA-1
chr3_4 five_prime_UTR 49863437 49863538 49863437 ID=Msa0504800-mRNA-1:five_prime_utr;Parent=Msa0504800-mRNA-1
chr3_4 five_prime_UTR 49864846 49864885 49864846 ID=Msa0504800-mRNA-1:five_prime_utr;Parent=Msa0504800-mRNA-1
chr3_4 CDS 49864886 49865214 49864886 ID=Msa0504800-mRNA-1:cds;Parent=Msa0504800-mRNA-1
chr3_4 CDS 49866508 49866517 49866508 ID=Msa0504800-mRNA-1:cds;Parent=Msa0504800-mRNA-1
chr3_4 three_prime_UTR 49866518 49867017 49866518 ID=Msa0504800-mRNA-1:three_prime_utr;Parent=Msa0504800-mRNA-1
Gene Sequence

>Msa0504800

ATGAACTCAGTTGTAAGACGTTTATCTAGCTTATTCAGACAGTCGGGTGGTGCAACTCAACCTTTTAATGCTCAGCATCATCAACTGCAGCAGCTTCAGCCATGCAGAGGCATACGAGTGCAAGTCTATAATGGAAACCTGGAAGGGGCATTGACATTGATGCAGCGTAAGATGACATCAAGTGGGATCGAGAGGATGATAAAGAAGGAACAGAGATTCCATATCAAAAACTCTGAGAAGCGTGTTTTAGCCCGAAAGAGCTTGGAGCGTAGGCTTCGATCAGAGGATCTTGCTAGGAAACTCAAATCCATTATGATCAAGAAAGTCAGGGGTCTTTGA
Protein sequence

>Msa0504800

MNSVVRRLSSLFRQSGGATQPFNAQHHQLQQLQPCRGIRVQVYNGNLEGALTLMQRKMTSSGIERMIKKEQRFHIKNSEKRVLARKSLERRLRSEDLARKLKSIMIKKVRGL*