AlfalfaGEDB | Msa0512070

M. sativa cultivar zhongmu-4 / Msa0512070

KEGG: K21374
GO: GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0008194 GO:0015020 GO:0016740 GO:0016757 GO:0016758 GO:0035251 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046527 GO:0050403 GO:0050502 GO:0080043 GO:0080044
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0512070	RHN68880.1	87.879	66	8	0	1	66	385	450	1.41e-31	124

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0512070	sp\|Q6VAB3\|U85A8_STERE	55.738	61	27	0	1	61	416	476	1.68e-16	74.7

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0512070	A0A396IU06	87.879	66	8	0	1	66	385	450	6.74e-32	124

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0012440	Msa0512070	0.805327	1.408600e-49	-8.615850e-47
Msa0512070	Msa1429420	0.809493	1.841315e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0512070	MtrunA17_Chr3g0118751	87.879	66	8	1	66	385	450	1.30e-35	124
Msa0512070	MtrunA17_Chr3g0118771	87.879	66	8	1	66	429	494	4.82e-35	123
Msa0512070	MtrunA17_Chr3g0118721	83.333	66	11	1	66	230	295	7.30e-33	114
Msa0512070	MtrunA17_Chr3g0118691	78.788	66	14	1	66	423	488	2.16e-32	115
Msa0512070	MtrunA17_Chr3g0118711	70.769	65	19	1	65	421	485	2.52e-29	107
Msa0512070	MtrunA17_Chr3g0118701	66.667	66	22	1	66	423	488	3.81e-27	101
Msa0512070	MtrunA17_Chr3g0118671	69.231	65	20	1	65	414	478	3.77e-26	98.6
Msa0512070	MtrunA17_Chr6g0454351	52.459	61	29	1	61	405	465	3.80e-17	73.2
Msa0512070	MtrunA17_Chr7g0217881	55.738	61	27	1	61	402	462	5.44e-17	72.8
Msa0512070	MtrunA17_Chr4g0050341	58.333	60	25	1	60	431	490	6.44e-17	72.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0512070	AT1G22400.1	46.774	62	32	1	1	61	421	482	6.58e-12	58.9
Msa0512070	AT1G22380.1	45.161	62	33	1	1	61	420	481	1.45e-11	57.8
Msa0512070	AT1G78270.1	43.548	62	34	1	1	61	420	481	2.06e-11	57.4
Msa0512070	AT1G22340.1	44.615	65	35	1	1	64	421	485	3.27e-11	57.0

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Find 12 sgRNAs with CRISPR-Local

Find 12 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATTAACTATGATGTGAAA+AGG	0.437634	3_4:+61743011	Msa0512070:CDS
AATTAACTATGATGTGAAAA+GGG	0.466340	3_4:+61743012	Msa0512070:CDS
ACAGATGCTACTAATGTGGG+AGG	0.700783	3_4:+61743124	Msa0512070:CDS
AGAGATAACCAAACTTGTTG+TGG	0.542980	3_4:+61743036	Msa0512070:CDS
AGAGATGAGACAAAATTGCT+TGG	0.504567	3_4:+61743084	Msa0512070:CDS
AGCTACAGATGCTACTAATG+TGG	0.548442	3_4:+61743120	Msa0512070:CDS
CAAACTTGTTGTGGAAATGA+TGG	0.488894	3_4:+61743045	Msa0512070:CDS
CATCATTTCCACAACAAGTT+TGG	0.310409	3_4:-61743044	None:intergenic
CTTGTTGTGGAAATGATGGA+AGG	0.654423	3_4:+61743049	Msa0512070:CDS
GAAATGATGGAAGGAGAAAA+AGG	0.360157	3_4:+61743058	Msa0512070:CDS

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CRISPR-GE

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badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CATCATTTCCACAACAAGTT+TGG	-	chr3_4:61743047-61743066	None:intergenic	35.0%
	AGAGATGAGACAAAATTGCT+TGG	+	chr3_4:61743084-61743103	Msa0512070:CDS	35.0%
	TGAGACAAAATTGCTTGGAG+TGG	+	chr3_4:61743089-61743108	Msa0512070:CDS	40.0%
	AGCTACAGATGCTACTAATG+TGG	+	chr3_4:61743120-61743139	Msa0512070:CDS	40.0%
	GCTACAGATGCTACTAATGT+GGG	+	chr3_4:61743121-61743140	Msa0512070:CDS	40.0%
	ACAGATGCTACTAATGTGGG+AGG	+	chr3_4:61743124-61743143	Msa0512070:CDS	45.0%
!	GAAATGATGGAAGGAGAAAA+AGG	+	chr3_4:61743058-61743077	Msa0512070:CDS	35.0%
!	CTTGTTGTGGAAATGATGGA+AGG	+	chr3_4:61743049-61743068	Msa0512070:CDS	40.0%
!!	AAATTAACTATGATGTGAAA+AGG	+	chr3_4:61743011-61743030	Msa0512070:CDS	20.0%
!!	AATTAACTATGATGTGAAAA+GGG	+	chr3_4:61743012-61743031	Msa0512070:CDS	20.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_4	gene	61743007	61743207	61743007	ID=Msa0512070;Name=Msa0512070
chr3_4	mRNA	61743007	61743207	61743007	ID=Msa0512070-mRNA-1;Parent=Msa0512070;Name=Msa0512070-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|66
chr3_4	exon	61743007	61743207	61743007	ID=Msa0512070-mRNA-1:exon:15107;Parent=Msa0512070-mRNA-1
chr3_4	CDS	61743007	61743207	61743007	ID=Msa0512070-mRNA-1:cds;Parent=Msa0512070-mRNA-1

Gene Sequence	>Msa0512070 ATGGAAATTAACTATGATGTGAAAAGGGAAGAGATAACCAAACTTGTTGTGGAAATGATGGAAGGAGAAAAAGGAAAAGAGATGAGACAAAATTGCTTGGAGTGGAAGAAGAAAGCTACAGATGCTACTAATGTGGGAGGATCATCATACAATAATTTCTATAATTTAATTAAAGAGCTTCTTCATCACAATGCTATTTGA
Protein sequence	>Msa0512070 MEINYDVKREEITKLVVEMMEGEKGKEMRQNCLEWKKKATDATNVGGSSYNNFYNLIKELLHHNAI*