AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0513420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0513420 MtrunA17_Chr7g0255111 70.312 576 160 5 1 572 1393 1961 0.0 764
Msa0513420 MtrunA17_Chr7g0255111 23.561 556 304 19 82 569 919 1421 3.50e-21 98.6
Msa0513420 MtrunA17_Chr5g0414911 63.402 582 200 7 2 572 1294 1873 0.0 696
Msa0513420 MtrunA17_Chr5g0414911 24.762 525 271 19 82 569 883 1320 2.62e-12 70.1
Msa0513420 MtrunA17_Chr1g0146241 63.793 580 197 6 2 572 1291 1866 0.0 676
Msa0513420 MtrunA17_Chr1g0146241 31.250 304 169 12 242 522 1235 1521 1.12e-21 100
Msa0513420 MtrunA17_Chr1g0146241 27.322 183 101 5 84 259 907 1064 2.03e-12 70.5
Msa0513420 MtrunA17_Chr7g0255391 73.554 363 87 4 212 572 2 357 1.50e-180 513
Msa0513420 MtrunA17_Chr7g0255391 33.511 188 108 6 33 215 118 293 7.38e-15 76.3
Msa0513420 MtrunA17_Chr7g0255401 50.980 204 93 4 2 199 1223 1425 3.38e-46 175
Msa0513420 MtrunA17_Chr7g0255401 28.653 349 182 10 137 471 1112 1407 6.10e-24 107
Msa0513420 MtrunA17_Chr7g0255401 25.628 199 101 5 76 259 834 1000 3.69e-11 66.2
Msa0513420 MtrunA17_Chr8g0359891 28.911 505 303 16 73 536 617 1106 3.55e-33 135
Msa0513420 MtrunA17_Chr8g0359891 28.398 493 270 22 78 541 348 786 5.02e-23 104
Msa0513420 MtrunA17_Chr8g0359891 25.247 507 268 17 78 541 1189 1627 1.06e-21 100
Msa0513420 MtrunA17_Chr8g0359891 25.856 526 274 16 80 565 2204 2653 2.16e-21 99.4
Msa0513420 MtrunA17_Chr8g0359891 24.758 517 320 17 68 568 2467 2930 8.21e-20 94.4
Msa0513420 MtrunA17_Chr8g0359891 27.990 418 209 17 135 541 1787 2123 1.17e-19 94.0
Msa0513420 MtrunA17_Chr8g0359891 41.216 148 79 3 73 218 1458 1599 5.46e-18 88.6
Msa0513420 MtrunA17_Chr8g0359891 23.321 536 302 22 51 552 79 539 2.75e-13 73.6
Msa0513420 MtrunA17_Chr8g0359891 38.739 111 64 1 111 221 1992 2098 1.54e-12 70.9
Msa0513420 MtrunA17_Chr1g0167981 26.444 450 238 16 112 539 1369 1747 1.03e-24 109
Msa0513420 MtrunA17_Chr1g0167981 28.251 446 280 17 112 536 800 1226 4.07e-21 98.6
Msa0513420 MtrunA17_Chr1g0167981 26.096 456 251 18 99 536 2402 2789 1.02e-20 97.4
Msa0513420 MtrunA17_Chr1g0167981 25.349 430 232 16 135 539 1907 2272 3.80e-20 95.5
Msa0513420 MtrunA17_Chr1g0167981 23.643 516 320 16 73 568 2105 2566 6.25e-18 88.2
Msa0513420 MtrunA17_Chr1g0167981 24.902 510 287 20 73 506 1580 2069 5.42e-15 79.0
Msa0513420 MtrunA17_Chr1g0167981 21.664 637 357 22 29 567 927 1519 1.89e-11 67.4
Msa0513420 MtrunA17_Chr2g0313461 27.061 473 285 18 116 557 815 1258 9.66e-24 106
Msa0513420 MtrunA17_Chr2g0313461 35.099 151 89 5 76 223 1077 1221 7.82e-13 71.6
Msa0513420 MtrunA17_Chr8g0348201 26.233 446 260 16 135 539 10 427 1.27e-23 106
Msa0513420 MtrunA17_Chr8g0348201 26.368 402 229 12 124 508 559 910 8.98e-22 100
Msa0513420 MtrunA17_Chr8g0348201 25.325 462 255 16 112 536 1061 1469 1.91e-21 99.4
Msa0513420 MtrunA17_Chr8g0348201 24.750 501 286 20 63 536 748 1184 4.64e-17 85.5
Msa0513420 MtrunA17_Chr1g0163761 26.499 517 264 17 89 565 1843 2283 1.93e-23 105
Msa0513420 MtrunA17_Chr1g0163761 25.941 478 243 17 109 541 1342 1753 7.32e-20 94.4
Msa0513420 MtrunA17_Chr1g0163761 25.000 520 322 18 68 568 2097 2567 1.28e-19 93.6
Msa0513420 MtrunA17_Chr1g0163761 41.216 148 79 3 73 218 1584 1725 3.16e-19 92.4
Msa0513420 MtrunA17_Chr1g0163761 23.762 505 279 18 81 552 1075 1506 2.93e-17 86.3
Msa0513420 MtrunA17_Chr1g0163761 27.273 440 286 13 115 536 809 1232 1.16e-16 84.3
Msa0513420 MtrunA17_Chr1g0164011 25.338 517 324 16 68 568 2019 2489 1.94e-23 105
Msa0513420 MtrunA17_Chr1g0164011 38.356 146 86 2 73 218 1583 1724 2.06e-17 86.7
Msa0513420 MtrunA17_Chr1g0164011 26.978 493 275 22 84 541 1310 1752 2.56e-17 86.3
Msa0513420 MtrunA17_Chr1g0164011 27.404 416 218 17 135 527 1835 2189 1.07e-16 84.3
Msa0513420 MtrunA17_Chr1g0164011 27.371 464 265 19 112 536 801 1231 7.91e-15 78.6
Msa0513420 MtrunA17_Chr1g0164011 23.014 491 269 16 81 536 1074 1490 1.17e-12 71.2
Msa0513420 MtrunA17_Chr2g0314301 25.849 530 286 20 73 568 1085 1541 2.55e-23 105
Msa0513420 MtrunA17_Chr2g0314301 27.002 437 262 14 112 508 805 1224 1.94e-20 96.3
Msa0513420 MtrunA17_Chr1g0163891 26.046 526 273 19 80 565 2367 2816 3.50e-23 105
Msa0513420 MtrunA17_Chr1g0163891 25.697 502 271 16 80 541 1845 2284 1.97e-22 102
Msa0513420 MtrunA17_Chr1g0163891 25.400 500 303 17 73 553 2115 2563 5.18e-22 101
Msa0513420 MtrunA17_Chr1g0163891 25.000 528 312 19 68 568 2630 3100 2.53e-20 95.9
Msa0513420 MtrunA17_Chr1g0163891 26.931 505 282 17 73 508 1595 2081 7.20e-20 94.7
Msa0513420 MtrunA17_Chr1g0163891 24.655 507 271 17 78 541 1326 1764 1.58e-18 90.1
Msa0513420 MtrunA17_Chr1g0163891 23.173 479 262 18 108 552 1111 1517 9.23e-12 68.6
Msa0513420 MtrunA17_Chr8g0348251 28.040 403 219 14 124 508 111 460 5.69e-23 103
Msa0513420 MtrunA17_Chr8g0348251 22.833 600 325 20 21 568 255 768 5.28e-16 81.6
Msa0513420 MtrunA17_Chr1g0172231 26.306 517 265 17 89 565 675 1115 7.74e-23 103
Msa0513420 MtrunA17_Chr1g0172231 24.248 532 311 19 68 568 929 1399 1.69e-19 93.2
Msa0513420 MtrunA17_Chr1g0172231 25.326 537 268 17 73 565 416 863 2.99e-18 89.4
Msa0513420 MtrunA17_Chr1g0172231 24.847 491 262 17 94 541 159 585 3.59e-18 89.0
Msa0513420 MtrunA17_Chr1g0163791 27.660 423 219 15 140 541 1392 1748 2.31e-22 102
Msa0513420 MtrunA17_Chr1g0163791 39.726 146 84 2 73 218 1579 1720 3.33e-18 89.0
Msa0513420 MtrunA17_Chr1g0163791 22.562 523 295 17 81 567 1070 1518 1.67e-13 73.9
Msa0513420 MtrunA17_Chr2g0313311 27.133 457 260 17 116 543 815 1227 2.56e-22 102
Msa0513420 MtrunA17_Chr2g0313311 23.979 563 316 22 43 568 1023 1510 2.18e-19 92.8
Msa0513420 MtrunA17_Chr2g0313591 26.786 448 264 15 116 534 815 1227 2.64e-22 102
Msa0513420 MtrunA17_Chr2g0313591 24.558 566 310 23 43 568 1043 1531 3.02e-21 98.6
Msa0513420 MtrunA17_Chr2g0313481 25.106 470 286 14 116 543 817 1262 3.34e-22 101
Msa0513420 MtrunA17_Chr2g0313481 24.468 564 313 23 43 568 1058 1546 3.81e-17 85.9
Msa0513420 MtrunA17_Chr1g0172051 25.956 497 255 17 80 536 2348 2771 3.55e-22 102
Msa0513420 MtrunA17_Chr1g0172051 24.852 507 258 18 78 536 1829 2260 9.76e-21 97.4
Msa0513420 MtrunA17_Chr1g0172051 24.085 519 284 14 73 556 1581 2024 2.95e-19 92.4
Msa0513420 MtrunA17_Chr1g0172051 27.181 493 279 21 78 543 1313 1752 1.72e-18 90.1
Msa0513420 MtrunA17_Chr1g0172051 25.572 481 281 14 68 511 2611 3051 2.37e-18 89.7
Msa0513420 MtrunA17_Chr1g0172051 30.729 384 223 16 115 472 806 1172 1.13e-17 87.4
Msa0513420 MtrunA17_Chr1g0172051 38.356 146 84 3 73 218 2098 2237 6.20e-17 85.1
Msa0513420 MtrunA17_Chr1g0172051 23.542 463 248 15 108 536 1098 1488 1.78e-11 67.8
Msa0513420 MtrunA17_Chr2g0313351 26.739 460 277 14 116 543 819 1250 4.72e-22 101
Msa0513420 MtrunA17_Chr2g0313351 24.286 560 318 20 43 568 1046 1533 2.56e-20 95.9
Msa0513420 MtrunA17_Chr2g0323691 25.947 528 262 18 73 553 2102 2547 7.40e-22 100
Msa0513420 MtrunA17_Chr2g0323691 26.061 495 264 19 89 543 1841 2273 1.41e-20 96.7
Msa0513420 MtrunA17_Chr2g0323691 25.701 428 231 17 135 541 1390 1751 2.14e-20 96.3
Msa0513420 MtrunA17_Chr2g0323691 24.419 516 273 17 90 565 2362 2800 7.84e-20 94.4
Msa0513420 MtrunA17_Chr2g0323691 39.189 148 86 3 73 220 1582 1725 1.51e-17 87.0
Msa0513420 MtrunA17_Chr2g0323691 23.755 522 326 18 68 568 2614 3084 5.71e-15 79.0
Msa0513420 MtrunA17_Chr2g0323691 25.221 452 281 17 115 536 806 1230 6.46e-14 75.5
Msa0513420 MtrunA17_Chr2g0323691 24.769 432 264 15 81 468 1073 1487 2.53e-11 67.0
Msa0513420 MtrunA17_Chr6g0474571 27.218 496 253 19 84 536 2356 2786 7.87e-22 100
Msa0513420 MtrunA17_Chr6g0474571 25.681 514 258 20 78 541 1312 1751 6.42e-20 94.7
Msa0513420 MtrunA17_Chr6g0474571 40.411 146 83 2 73 218 1582 1723 2.33e-18 89.7
Msa0513420 MtrunA17_Chr6g0474571 26.622 447 280 16 115 536 806 1229 1.60e-16 84.0
Msa0513420 MtrunA17_Chr6g0474571 36.612 183 100 5 50 222 2071 2247 2.93e-13 73.6
Msa0513420 MtrunA17_Chr6g0474571 31.613 155 100 2 68 220 2615 2765 1.29e-12 71.2
Msa0513420 MtrunA17_Chr6g0474571 26.061 495 258 19 90 541 1842 2271 2.13e-12 70.5
Msa0513420 MtrunA17_Chr2g0313331 24.436 532 291 20 73 568 767 1223 5.71e-21 97.8
Msa0513420 MtrunA17_Chr2g0313331 24.356 427 272 12 116 508 497 906 9.92e-18 87.4
Msa0513420 MtrunA17_Chr2g0313541 23.886 561 323 22 43 570 1051 1540 8.36e-21 97.4
Msa0513420 MtrunA17_Chr1g0174511 28.392 398 194 15 135 508 1387 1717 1.76e-20 96.3
Msa0513420 MtrunA17_Chr1g0174511 38.356 146 86 2 73 218 1579 1720 8.97e-18 87.8
Msa0513420 MtrunA17_Chr1g0174511 27.551 392 234 13 112 472 797 1169 4.20e-17 85.5
Msa0513420 MtrunA17_Chr1g0174511 22.682 507 282 16 81 552 1070 1501 5.31e-14 75.5
Msa0513420 MtrunA17_Chr2g0313421 23.488 562 320 18 43 568 153 640 3.20e-20 94.7
Msa0513420 MtrunA17_Chr2g0313421 25.945 397 225 16 176 543 1 357 6.36e-14 74.7
Msa0513420 MtrunA17_Chr1g0163931 26.336 524 307 20 68 568 1574 2041 6.22e-20 94.7
Msa0513420 MtrunA17_Chr1g0163931 25.000 512 258 19 99 565 1329 1759 1.74e-18 90.1
Msa0513420 MtrunA17_Chr1g0163931 24.046 524 284 19 82 567 1073 1520 3.95e-17 85.9
Msa0513420 MtrunA17_Chr3g0081941 24.528 477 281 21 116 550 807 1246 1.33e-18 90.1
Msa0513420 MtrunA17_Chr3g0081941 25.862 232 142 5 43 272 1040 1243 3.94e-15 79.3
Msa0513420 MtrunA17_Chr1g0170881 26.995 426 281 12 129 536 815 1228 3.15e-18 89.4
Msa0513420 MtrunA17_Chr1g0170881 23.866 507 274 18 82 552 1072 1502 2.87e-17 86.3
Msa0513420 MtrunA17_Chr1g0170881 26.411 496 272 22 99 565 1328 1759 1.61e-15 80.5
Msa0513420 MtrunA17_Chr1g0170881 25.380 461 271 14 86 511 1593 2015 4.26e-12 69.7
Msa0513420 MtrunA17_Chr8g0359871 39.855 138 79 2 81 218 5 138 3.64e-18 84.0
Msa0513420 MtrunA17_Chr8g0359871 30.337 178 100 8 375 541 2 166 5.02e-12 65.9
Msa0513420 MtrunA17_Chr1g0167331 25.705 319 195 8 73 390 35 312 2.05e-17 84.0
Msa0513420 MtrunA17_Chr1g0172081 36.301 146 89 1 73 218 57 198 6.31e-17 81.3
Msa0513420 MtrunA17_Chr1g0172081 30.978 184 109 6 368 543 55 228 8.70e-14 72.0
Msa0513420 MtrunA17_Chr2g0314141 36.486 148 85 2 73 218 18 158 9.16e-17 79.0
Msa0513420 MtrunA17_Chr1g0163801 25.806 496 276 18 73 552 1 420 6.10e-16 80.5
Msa0513420 MtrunA17_Chr1g0172141 28.982 383 233 14 115 472 805 1173 1.89e-15 80.5
Msa0513420 MtrunA17_Chr2g0313471 24.615 260 112 8 86 317 47 250 1.32e-13 71.2
Msa0513420 MtrunA17_Chr2g0313471 26.641 259 164 11 321 568 8 251 1.97e-11 64.7
Msa0513420 MtrunA17_Chr2g0313491 25.659 417 230 18 111 462 1079 1480 1.92e-13 73.9
Msa0513420 MtrunA17_Chr3g0108011 23.298 382 238 17 139 511 1084 1419 7.73e-13 72.0
Msa0513420 MtrunA17_Chr1g0163991 24.190 401 208 14 135 494 429 774 1.89e-12 70.5
Msa0513420 MtrunA17_Chr1g0163991 23.121 519 293 19 81 567 816 1260 2.00e-12 70.5
Msa0513420 MtrunA17_Chr1g0163991 22.458 472 288 18 51 468 79 526 1.94e-11 67.4
Msa0513420 MtrunA17_Chr1g0163991 29.688 192 122 5 351 536 85 269 6.14e-11 65.9
Msa0513420 MtrunA17_Chr1g0164101 29.016 193 124 5 350 536 15 200 2.16e-12 67.4
Msa0513420 MtrunA17_Chr1g0160951 23.394 436 243 19 137 533 841 1224 5.39e-12 69.3
Msa0513420 MtrunA17_Chr1g0170961 46.341 82 40 2 137 218 3 80 2.55e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 113 sgRNAs with CRISPR-Local

Find 282 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATACACTCCAGGTCTTTA+TGG 0.128058 3_4:+63653589 Msa0513420:CDS
CTTGAAAACACTTCCAATTT+AGG 0.136657 3_4:-63651692 None:intergenic
TGGGGAAAGGTTGTCATATT+TGG 0.166084 3_4:-63652704 None:intergenic
TTCCACAATTAAAGGAATTA+TGG 0.199351 3_4:+63652600 Msa0513420:CDS
TTGAAGTTGCACAAGGATTT+TGG 0.204790 3_4:-63652345 None:intergenic
TCCCAAAAGGTCTTCATCTT+TGG 0.207223 3_4:-63653556 None:intergenic
CCTCTTCAACAGTGCATAAA+TGG 0.226416 3_4:-63652538 None:intergenic
GAGAAGATAGAGGAGATAAT+TGG 0.229877 3_4:+63653358 Msa0513420:CDS
GATAATATATTGTCAAATTT+AGG 0.245414 3_4:-63652802 None:intergenic
ACATAAATCGAATTCAAATT+TGG 0.246350 3_4:-63653325 None:intergenic
GATTTCATTTCCACAATTAA+AGG 0.250376 3_4:+63652592 Msa0513420:CDS
TAGGATTTGAGTGTTTAAAA+AGG 0.284011 3_4:+63653236 Msa0513420:CDS
ATCTTCATCTTTGTCAAATT+CGG 0.284428 3_4:-63653629 None:intergenic
TATTGAAGATTGTCCTAAAT+TGG 0.291652 3_4:+63651679 Msa0513420:five_prime_UTR
GCTGAGTGATTTAATGTTTA+CGG 0.293016 3_4:-63651750 None:intergenic
AGAGCAAACTCTTCAAATTA+TGG 0.294790 3_4:-63652310 None:intergenic
ATTAGAGACGTTTAGAGATA+TGG 0.301244 3_4:+63652961 Msa0513420:CDS
GCCCAAAGATGAAGACCTTT+TGG 0.304326 3_4:+63653554 Msa0513420:CDS
AAGACATAAATTCCGTTATA+CGG 0.311113 3_4:+63651792 Msa0513420:CDS
AAACATCATGGTCAGATTAT+AGG 0.318295 3_4:+63653217 Msa0513420:CDS
TTCCAGATGTGCTTCAGTTT+TGG 0.323768 3_4:-63653196 None:intergenic
TTGAGATGCCACGATGTTAT+AGG 0.326081 3_4:+63653515 Msa0513420:CDS
GATTCACTAGTCGAGGTGTT+TGG 0.333299 3_4:+63652128 Msa0513420:CDS
CCCAAAAGGTCTTCATCTTT+GGG 0.337764 3_4:-63653555 None:intergenic
TTCCAAAACTGAAGCACATC+TGG 0.338477 3_4:+63653194 Msa0513420:CDS
GTATCCCTCGTTATGTTGTT+TGG 0.363796 3_4:-63651997 None:intergenic
TGTCAAAATTGTGTCACATA+TGG 0.367717 3_4:+63652225 Msa0513420:CDS
ATTCACTAGTCGAGGTGTTT+GGG 0.386845 3_4:+63652129 Msa0513420:CDS
CAAAGAATTTCATAAATTGT+TGG 0.403450 3_4:+63652046 Msa0513420:CDS
AGAAGATAGAGGAGATAATT+GGG 0.422919 3_4:+63653359 Msa0513420:CDS
CTAGTCGAGGTGTTTGGGTC+AGG 0.422966 3_4:+63652134 Msa0513420:CDS
GATATGGATGAAGAGCTTCT+TGG 0.434016 3_4:+63652977 Msa0513420:CDS
AGCTTTGACTGAAACATTTG+AGG 0.435968 3_4:-63653477 None:intergenic
TGATTTGAAATATGTGCTTC+CGG 0.437363 3_4:+63653279 Msa0513420:CDS
AAGGATTGGAGATGTTGAAT+TGG 0.441240 3_4:+63651973 Msa0513420:CDS
AGTTCTATATACAGCAATGA+TGG 0.443009 3_4:-63653439 None:intergenic
TGGAAGAAGGCCTTAAGGTT+GGG 0.454022 3_4:-63651614 None:intergenic
GACCTTGGAAGAAGGCCTTA+AGG 0.466377 3_4:-63651619 None:intergenic
AGCCATAATTCCTTTAATTG+TGG 0.467821 3_4:-63652602 None:intergenic
AGTGACAATTCCTCCAGCTT+AGG 0.470279 3_4:-63652461 None:intergenic
ACAGGTGGCATCACAAGGAT+TGG 0.471384 3_4:+63651959 Msa0513420:intron
CACCGTAGATGATGAAGGTT+GGG 0.472044 3_4:-63652749 None:intergenic
GCATGTTGCAGTGTGAACTA+TGG 0.477053 3_4:+63653152 Msa0513420:CDS
TGTTTACAATAACATTTCCA+TGG 0.479375 3_4:-63652724 None:intergenic
TTCATCATCTACGGTGGGAG+TGG 0.481204 3_4:+63652756 Msa0513420:CDS
GGAATTCAATAACAATGATA+AGG 0.490365 3_4:+63652565 Msa0513420:CDS
AATCAAGATTATAGTGACCT+TGG 0.490619 3_4:-63651634 None:intergenic
AGACATAAATTCCGTTATAC+GGG 0.492080 3_4:+63651793 Msa0513420:CDS
TGTTGAAGCATATGTATCTC+AGG 0.493850 3_4:-63652077 None:intergenic
GAAGATGAAAATATAAAAGA+AGG 0.496853 3_4:+63652416 Msa0513420:CDS
TTGGAAGAAGGCCTTAAGGT+TGG 0.499907 3_4:-63651615 None:intergenic
TAAGATTCCTTCGTCCCAAA+AGG 0.504491 3_4:-63653569 None:intergenic
CTCCTGCAGTTGATAATGAA+TGG 0.510077 3_4:-63652186 None:intergenic
TGACTGAAACATTTGAGGCT+AGG 0.512933 3_4:-63653472 None:intergenic
AACCTTAAGGCCTTCTTCCA+AGG 0.513224 3_4:+63651617 Msa0513420:five_prime_UTR
CCATTTATGCACTGTTGAAG+AGG 0.513344 3_4:+63652538 Msa0513420:CDS
GTGTTTGGGTCAGGAGGAGA+AGG 0.515595 3_4:+63652143 Msa0513420:CDS
AAATCGAATTCAAATTTGGA+AGG 0.520534 3_4:-63653321 None:intergenic
GTTTACAATAACATTTCCAT+GGG 0.521481 3_4:-63652723 None:intergenic
AATAACATTTCCATGGGGAA+AGG 0.523863 3_4:-63652717 None:intergenic
AATAATTGCATCATGTTGGA+TGG 0.525133 3_4:-63653061 None:intergenic
TCATCATCTACGGTGGGAGT+GGG 0.526916 3_4:+63652757 Msa0513420:CDS
TACCATTCATTATCAACTGC+AGG 0.530426 3_4:+63652184 Msa0513420:CDS
CACCCAACCTTCATCATCTA+CGG 0.534491 3_4:+63652747 Msa0513420:CDS
TCAACAGCTACCCAACCTTA+AGG 0.540693 3_4:+63651604 None:intergenic
ATTATAGTGACCTTGGAAGA+AGG 0.546507 3_4:-63651627 None:intergenic
CCACCGTAGATGATGAAGGT+TGG 0.548462 3_4:-63652750 None:intergenic
ACGGTGAGATCCTCAAGCAT+GGG 0.557001 3_4:-63651731 None:intergenic
ATTCAAATTTGGAAGGCTTG+TGG 0.561635 3_4:-63653314 None:intergenic
TACGGTGAGATCCTCAAGCA+TGG 0.563629 3_4:-63651732 None:intergenic
GTCTGAATGCGAGAAGATAG+AGG 0.568434 3_4:+63653348 Msa0513420:CDS
TGAGAATAATTGCATCATGT+TGG 0.570700 3_4:-63653065 None:intergenic
CGTGGCATCTCAACATAGGA+AGG 0.572635 3_4:-63653505 None:intergenic
GTTCTATATACAGCAATGAT+GGG 0.572835 3_4:-63653438 None:intergenic
ACATCGTGGCATCTCAACAT+AGG 0.577369 3_4:-63653509 None:intergenic
TTGTTAGCCTTGTGTTGTTG+CGG 0.577399 3_4:-63653406 None:intergenic
CTTGTGGCTATTGAAACATC+CGG 0.577470 3_4:-63653298 None:intergenic
AGTGTACGACTACGATATCA+TGG 0.582757 3_4:+63652661 Msa0513420:CDS
GTCGAGGTGTTTGGGTCAGG+AGG 0.583099 3_4:+63652137 Msa0513420:CDS
GAAGGAATCTTATACACTCC+AGG 0.584264 3_4:+63653580 Msa0513420:CDS
GAGTTTGCTCTCTATATCCA+TGG 0.584354 3_4:+63652322 Msa0513420:CDS
AGTTGAGTCTTGCAACATGA+TGG 0.586435 3_4:+63652376 Msa0513420:CDS
TGGGGATATAAACAAACACT+TGG 0.595165 3_4:+63652776 Msa0513420:CDS
ACTCCCACCGTAGATGATGA+AGG 0.600393 3_4:-63652754 None:intergenic
CATCATCTACGGTGGGAGTG+GGG 0.604922 3_4:+63652758 Msa0513420:CDS
GATGTTAACGAGGTGATACA+AGG 0.609419 3_4:+63653649 Msa0513420:CDS
AAGCACATCTGGAAACATCA+TGG 0.610576 3_4:+63653205 Msa0513420:CDS
TGTTGAACCGCAACAACACA+AGG 0.614781 3_4:+63653399 Msa0513420:CDS
ATGCACTGTTGAAGAGGACA+AGG 0.614975 3_4:+63652544 Msa0513420:CDS
ATAATTGCATCATGTTGGAT+GGG 0.615576 3_4:-63653060 None:intergenic
TGCAATTATTCTCACATGTG+AGG 0.616272 3_4:+63653074 Msa0513420:CDS
CGGTGAGATCCTCAAGCATG+GGG 0.619271 3_4:-63651730 None:intergenic
GACATAAATTCCGTTATACG+GGG 0.624135 3_4:+63651794 Msa0513420:CDS
TGATTGTGATTCACTAGTCG+AGG 0.631404 3_4:+63652121 Msa0513420:CDS
GATGGATGAGATAACTACAA+AGG 0.638189 3_4:+63652394 Msa0513420:CDS
CAACCTTCATCATCTACGGT+GGG 0.640470 3_4:+63652751 Msa0513420:CDS
CATGTGAGGTATCTCACAGT+GGG 0.642706 3_4:+63653088 Msa0513420:CDS
ACAGAAATTCCATAAAGACC+TGG 0.646836 3_4:-63653598 None:intergenic
AATATGACAACCTTTCCCCA+TGG 0.649695 3_4:+63652707 Msa0513420:CDS
CCAACCTTCATCATCTACGG+TGG 0.651590 3_4:+63652750 Msa0513420:CDS
CAAAGATGAAGATGTTAACG+AGG 0.652261 3_4:+63653639 Msa0513420:CDS
GTGGCATCTCAACATAGGAA+GGG 0.657119 3_4:-63653504 None:intergenic
TCATAATTCAGAGAAATACA+AGG 0.668872 3_4:+63652826 Msa0513420:CDS
GAAGGTAGAAAAGAATACGA+TGG 0.679715 3_4:+63652161 Msa0513420:CDS
ATGGAAACATAATATTACCA+AGG 0.685404 3_4:+63652244 Msa0513420:CDS
ATTCTTATCCTATAACATCG+TGG 0.687403 3_4:-63653523 None:intergenic
ACATAAATTCCGTTATACGG+GGG 0.688318 3_4:+63651795 Msa0513420:CDS
TTGGACCAAACAACATAACG+AGG 0.692430 3_4:+63651992 Msa0513420:CDS
TTTACAATAACATTTCCATG+GGG 0.698915 3_4:-63652722 None:intergenic
TGGACCAAACAACATAACGA+GGG 0.701795 3_4:+63651993 Msa0513420:CDS
TCAGCAGCAACTATATACAG+AGG 0.712255 3_4:+63651768 Msa0513420:CDS
ACATGTGAGGTATCTCACAG+TGG 0.739112 3_4:+63653087 Msa0513420:CDS
GGTGAGATCCTCAAGCATGG+GGG 0.752578 3_4:-63651729 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTATTGAACATATATG+TGG - chr3_4:63651893-63651912 None:intergenic 15.0%
!! GATAATATATTGTCAAATTT+AGG - chr3_4:63652805-63652824 None:intergenic 15.0%
!! TTAATTATTGAACATATATG+TGG - chr3_4:63651893-63651912 None:intergenic 15.0%
!! GATAATATATTGTCAAATTT+AGG - chr3_4:63652805-63652824 None:intergenic 15.0%
!! GAAAGTAATAATTTACTTGT+GGG - chr3_4:63651862-63651881 None:intergenic 20.0%
!! AGAAAGTAATAATTTACTTG+TGG - chr3_4:63651863-63651882 None:intergenic 20.0%
!! CAAAGAATTTCATAAATTGT+TGG + chr3_4:63652046-63652065 Msa0513420:CDS 20.0%
!!! CACTAATATCTTTTAGATTT+TGG - chr3_4:63652280-63652299 None:intergenic 20.0%
!! GAAGATGAAAATATAAAAGA+AGG + chr3_4:63652416-63652435 Msa0513420:CDS 20.0%
!! AAAATTAATTGAGTAGTTTG+AGG - chr3_4:63652882-63652901 None:intergenic 20.0%
!! ACATAAATCGAATTCAAATT+TGG - chr3_4:63653328-63653347 None:intergenic 20.0%
!! GAAAGTAATAATTTACTTGT+GGG - chr3_4:63651862-63651881 None:intergenic 20.0%
!! AGAAAGTAATAATTTACTTG+TGG - chr3_4:63651863-63651882 None:intergenic 20.0%
!! CAAAGAATTTCATAAATTGT+TGG + chr3_4:63652046-63652065 Msa0513420:CDS 20.0%
!!! CACTAATATCTTTTAGATTT+TGG - chr3_4:63652280-63652299 None:intergenic 20.0%
!! GAAGATGAAAATATAAAAGA+AGG + chr3_4:63652416-63652435 Msa0513420:CDS 20.0%
!! AAAATTAATTGAGTAGTTTG+AGG - chr3_4:63652882-63652901 None:intergenic 20.0%
!! ACATAAATCGAATTCAAATT+TGG - chr3_4:63653328-63653347 None:intergenic 20.0%
!!! ATACACACTTTTTTCAATGA+TGG - chr3_4:63651662-63651681 None:intergenic 25.0%
! TATTGAAGATTGTCCTAAAT+TGG + chr3_4:63651679-63651698 Msa0513420:five_prime_UTR 25.0%
! AAGACATAAATTCCGTTATA+CGG + chr3_4:63651792-63651811 Msa0513420:CDS 25.0%
!!! GATAACCATATTTGTTTTAC+AGG + chr3_4:63651941-63651960 Msa0513420:intron 25.0%
! ATGGAAACATAATATTACCA+AGG + chr3_4:63652244-63652263 Msa0513420:CDS 25.0%
! GGAATTCAATAACAATGATA+AGG + chr3_4:63652565-63652584 Msa0513420:CDS 25.0%
! GATTTCATTTCCACAATTAA+AGG + chr3_4:63652592-63652611 Msa0513420:CDS 25.0%
! TTCCACAATTAAAGGAATTA+TGG + chr3_4:63652600-63652619 Msa0513420:CDS 25.0%
! TTTACAATAACATTTCCATG+GGG - chr3_4:63652725-63652744 None:intergenic 25.0%
! GTTTACAATAACATTTCCAT+GGG - chr3_4:63652726-63652745 None:intergenic 25.0%
! TGTTTACAATAACATTTCCA+TGG - chr3_4:63652727-63652746 None:intergenic 25.0%
! TCATAATTCAGAGAAATACA+AGG + chr3_4:63652826-63652845 Msa0513420:CDS 25.0%
! TAGGATTTGAGTGTTTAAAA+AGG + chr3_4:63653236-63653255 Msa0513420:CDS 25.0%
! AAATCGAATTCAAATTTGGA+AGG - chr3_4:63653324-63653343 None:intergenic 25.0%
! ATCTTCATCTTTGTCAAATT+CGG - chr3_4:63653632-63653651 None:intergenic 25.0%
!!! ATACACACTTTTTTCAATGA+TGG - chr3_4:63651662-63651681 None:intergenic 25.0%
! TATTGAAGATTGTCCTAAAT+TGG + chr3_4:63651679-63651698 Msa0513420:five_prime_UTR 25.0%
! AAGACATAAATTCCGTTATA+CGG + chr3_4:63651792-63651811 Msa0513420:CDS 25.0%
!!! GATAACCATATTTGTTTTAC+AGG + chr3_4:63651941-63651960 Msa0513420:intron 25.0%
! ATGGAAACATAATATTACCA+AGG + chr3_4:63652244-63652263 Msa0513420:CDS 25.0%
! GGAATTCAATAACAATGATA+AGG + chr3_4:63652565-63652584 Msa0513420:CDS 25.0%
! GATTTCATTTCCACAATTAA+AGG + chr3_4:63652592-63652611 Msa0513420:CDS 25.0%
! TTCCACAATTAAAGGAATTA+TGG + chr3_4:63652600-63652619 Msa0513420:CDS 25.0%
! TTTACAATAACATTTCCATG+GGG - chr3_4:63652725-63652744 None:intergenic 25.0%
! GTTTACAATAACATTTCCAT+GGG - chr3_4:63652726-63652745 None:intergenic 25.0%
! TGTTTACAATAACATTTCCA+TGG - chr3_4:63652727-63652746 None:intergenic 25.0%
! TCATAATTCAGAGAAATACA+AGG + chr3_4:63652826-63652845 Msa0513420:CDS 25.0%
! TAGGATTTGAGTGTTTAAAA+AGG + chr3_4:63653236-63653255 Msa0513420:CDS 25.0%
! AAATCGAATTCAAATTTGGA+AGG - chr3_4:63653324-63653343 None:intergenic 25.0%
! ATCTTCATCTTTGTCAAATT+CGG - chr3_4:63653632-63653651 None:intergenic 25.0%
AATCAAGATTATAGTGACCT+TGG - chr3_4:63651637-63651656 None:intergenic 30.0%
CTTGAAAACACTTCCAATTT+AGG - chr3_4:63651695-63651714 None:intergenic 30.0%
!!! AAATTGGAAGTGTTTTCAAG+AGG + chr3_4:63651695-63651714 Msa0513420:five_prime_UTR 30.0%
GCTGAGTGATTTAATGTTTA+CGG - chr3_4:63651753-63651772 None:intergenic 30.0%
AGACATAAATTCCGTTATAC+GGG + chr3_4:63651793-63651812 Msa0513420:CDS 30.0%
!!! AACCATATTTGTTTTACAGG+TGG + chr3_4:63651944-63651963 Msa0513420:intron 30.0%
!! TGTGACACAATTTTGACAAA+CGG - chr3_4:63652223-63652242 None:intergenic 30.0%
TGTCAAAATTGTGTCACATA+TGG + chr3_4:63652225-63652244 Msa0513420:CDS 30.0%
AGAGCAAACTCTTCAAATTA+TGG - chr3_4:63652313-63652332 None:intergenic 30.0%
!! GAAGATTTTATTTCCTAAGC+TGG + chr3_4:63652448-63652467 Msa0513420:CDS 30.0%
GAACAAAAACATTTCAGCTT+TGG - chr3_4:63652497-63652516 None:intergenic 30.0%
!! AAGCTGAAATGTTTTTGTTC+AGG + chr3_4:63652497-63652516 Msa0513420:CDS 30.0%
AGCCATAATTCCTTTAATTG+TGG - chr3_4:63652605-63652624 None:intergenic 30.0%
ATTAATTGAGTAGTTTGAGG+TGG - chr3_4:63652879-63652898 None:intergenic 30.0%
TATACGTTGTATCTTTGTGT+AGG + chr3_4:63652925-63652944 Msa0513420:intron 30.0%
ATTAGAGACGTTTAGAGATA+TGG + chr3_4:63652961-63652980 Msa0513420:CDS 30.0%
ATAATTGCATCATGTTGGAT+GGG - chr3_4:63653063-63653082 None:intergenic 30.0%
AATAATTGCATCATGTTGGA+TGG - chr3_4:63653064-63653083 None:intergenic 30.0%
TGAGAATAATTGCATCATGT+TGG - chr3_4:63653068-63653087 None:intergenic 30.0%
AAACATCATGGTCAGATTAT+AGG + chr3_4:63653217-63653236 Msa0513420:CDS 30.0%
! TGATTTGAAATATGTGCTTC+CGG + chr3_4:63653279-63653298 Msa0513420:CDS 30.0%
AGAAGATAGAGGAGATAATT+GGG + chr3_4:63653359-63653378 Msa0513420:CDS 30.0%
GTTCTATATACAGCAATGAT+GGG - chr3_4:63653441-63653460 None:intergenic 30.0%
AGTTCTATATACAGCAATGA+TGG - chr3_4:63653442-63653461 None:intergenic 30.0%
ATTCTTATCCTATAACATCG+TGG - chr3_4:63653526-63653545 None:intergenic 30.0%
AATCAAGATTATAGTGACCT+TGG - chr3_4:63651637-63651656 None:intergenic 30.0%
CTTGAAAACACTTCCAATTT+AGG - chr3_4:63651695-63651714 None:intergenic 30.0%
!!! AAATTGGAAGTGTTTTCAAG+AGG + chr3_4:63651695-63651714 Msa0513420:five_prime_UTR 30.0%
GCTGAGTGATTTAATGTTTA+CGG - chr3_4:63651753-63651772 None:intergenic 30.0%
AGACATAAATTCCGTTATAC+GGG + chr3_4:63651793-63651812 Msa0513420:CDS 30.0%
!!! AACCATATTTGTTTTACAGG+TGG + chr3_4:63651944-63651963 Msa0513420:intron 30.0%
!! TGTGACACAATTTTGACAAA+CGG - chr3_4:63652223-63652242 None:intergenic 30.0%
TGTCAAAATTGTGTCACATA+TGG + chr3_4:63652225-63652244 Msa0513420:CDS 30.0%
AGAGCAAACTCTTCAAATTA+TGG - chr3_4:63652313-63652332 None:intergenic 30.0%
!! GAAGATTTTATTTCCTAAGC+TGG + chr3_4:63652448-63652467 Msa0513420:CDS 30.0%
GAACAAAAACATTTCAGCTT+TGG - chr3_4:63652497-63652516 None:intergenic 30.0%
!! AAGCTGAAATGTTTTTGTTC+AGG + chr3_4:63652497-63652516 Msa0513420:CDS 30.0%
AGCCATAATTCCTTTAATTG+TGG - chr3_4:63652605-63652624 None:intergenic 30.0%
ATTAATTGAGTAGTTTGAGG+TGG - chr3_4:63652879-63652898 None:intergenic 30.0%
TATACGTTGTATCTTTGTGT+AGG + chr3_4:63652925-63652944 Msa0513420:intron 30.0%
ATTAGAGACGTTTAGAGATA+TGG + chr3_4:63652961-63652980 Msa0513420:CDS 30.0%
ATAATTGCATCATGTTGGAT+GGG - chr3_4:63653063-63653082 None:intergenic 30.0%
AATAATTGCATCATGTTGGA+TGG - chr3_4:63653064-63653083 None:intergenic 30.0%
TGAGAATAATTGCATCATGT+TGG - chr3_4:63653068-63653087 None:intergenic 30.0%
AAACATCATGGTCAGATTAT+AGG + chr3_4:63653217-63653236 Msa0513420:CDS 30.0%
! TGATTTGAAATATGTGCTTC+CGG + chr3_4:63653279-63653298 Msa0513420:CDS 30.0%
AGAAGATAGAGGAGATAATT+GGG + chr3_4:63653359-63653378 Msa0513420:CDS 30.0%
GTTCTATATACAGCAATGAT+GGG - chr3_4:63653441-63653460 None:intergenic 30.0%
AGTTCTATATACAGCAATGA+TGG - chr3_4:63653442-63653461 None:intergenic 30.0%
ATTCTTATCCTATAACATCG+TGG - chr3_4:63653526-63653545 None:intergenic 30.0%
ATTATAGTGACCTTGGAAGA+AGG - chr3_4:63651630-63651649 None:intergenic 35.0%
GACATAAATTCCGTTATACG+GGG + chr3_4:63651794-63651813 Msa0513420:CDS 35.0%
ACATAAATTCCGTTATACGG+GGG + chr3_4:63651795-63651814 Msa0513420:CDS 35.0%
TGATTTAAACCCCCGTATAA+CGG - chr3_4:63651807-63651826 None:intergenic 35.0%
TGCCACCTGTAAAACAAATA+TGG - chr3_4:63651949-63651968 None:intergenic 35.0%
AAGGATTGGAGATGTTGAAT+TGG + chr3_4:63651973-63651992 Msa0513420:CDS 35.0%
TGTTGAAGCATATGTATCTC+AGG - chr3_4:63652080-63652099 None:intergenic 35.0%
GAAGGTAGAAAAGAATACGA+TGG + chr3_4:63652161-63652180 Msa0513420:CDS 35.0%
TACCATTCATTATCAACTGC+AGG + chr3_4:63652184-63652203 Msa0513420:CDS 35.0%
!! ATTTTGGAAGCTTACAACCT+TGG - chr3_4:63652264-63652283 None:intergenic 35.0%
! TTGAAGTTGCACAAGGATTT+TGG - chr3_4:63652348-63652367 None:intergenic 35.0%
!!! GTAGTTTTTGAAGTTGCACA+AGG - chr3_4:63652355-63652374 None:intergenic 35.0%
GATGGATGAGATAACTACAA+AGG + chr3_4:63652394-63652413 Msa0513420:CDS 35.0%
!! GATTTTATTTCCTAAGCTGG+AGG + chr3_4:63652451-63652470 Msa0513420:CDS 35.0%
AATAACATTTCCATGGGGAA+AGG - chr3_4:63652720-63652739 None:intergenic 35.0%
TGGGGATATAAACAAACACT+TGG + chr3_4:63652776-63652795 Msa0513420:CDS 35.0%
TACAAACACATACATGCATG+AGG + chr3_4:63652849-63652868 Msa0513420:intron 35.0%
TGCAATTATTCTCACATGTG+AGG + chr3_4:63653074-63653093 Msa0513420:CDS 35.0%
ATTCAAATTTGGAAGGCTTG+TGG - chr3_4:63653317-63653336 None:intergenic 35.0%
GAGAAGATAGAGGAGATAAT+TGG + chr3_4:63653358-63653377 Msa0513420:CDS 35.0%
! AGCTTTGACTGAAACATTTG+AGG - chr3_4:63653480-63653499 None:intergenic 35.0%
TTATACACTCCAGGTCTTTA+TGG + chr3_4:63653589-63653608 Msa0513420:CDS 35.0%
ACAGAAATTCCATAAAGACC+TGG - chr3_4:63653601-63653620 None:intergenic 35.0%
CAAAGATGAAGATGTTAACG+AGG + chr3_4:63653639-63653658 Msa0513420:CDS 35.0%
ATTATAGTGACCTTGGAAGA+AGG - chr3_4:63651630-63651649 None:intergenic 35.0%
GACATAAATTCCGTTATACG+GGG + chr3_4:63651794-63651813 Msa0513420:CDS 35.0%
ACATAAATTCCGTTATACGG+GGG + chr3_4:63651795-63651814 Msa0513420:CDS 35.0%
TGATTTAAACCCCCGTATAA+CGG - chr3_4:63651807-63651826 None:intergenic 35.0%
TGCCACCTGTAAAACAAATA+TGG - chr3_4:63651949-63651968 None:intergenic 35.0%
AAGGATTGGAGATGTTGAAT+TGG + chr3_4:63651973-63651992 Msa0513420:CDS 35.0%
TGTTGAAGCATATGTATCTC+AGG - chr3_4:63652080-63652099 None:intergenic 35.0%
GAAGGTAGAAAAGAATACGA+TGG + chr3_4:63652161-63652180 Msa0513420:CDS 35.0%
TACCATTCATTATCAACTGC+AGG + chr3_4:63652184-63652203 Msa0513420:CDS 35.0%
!! ATTTTGGAAGCTTACAACCT+TGG - chr3_4:63652264-63652283 None:intergenic 35.0%
! TTGAAGTTGCACAAGGATTT+TGG - chr3_4:63652348-63652367 None:intergenic 35.0%
!!! GTAGTTTTTGAAGTTGCACA+AGG - chr3_4:63652355-63652374 None:intergenic 35.0%
GATGGATGAGATAACTACAA+AGG + chr3_4:63652394-63652413 Msa0513420:CDS 35.0%
!! GATTTTATTTCCTAAGCTGG+AGG + chr3_4:63652451-63652470 Msa0513420:CDS 35.0%
AATAACATTTCCATGGGGAA+AGG - chr3_4:63652720-63652739 None:intergenic 35.0%
TGGGGATATAAACAAACACT+TGG + chr3_4:63652776-63652795 Msa0513420:CDS 35.0%
TACAAACACATACATGCATG+AGG + chr3_4:63652849-63652868 Msa0513420:intron 35.0%
TGCAATTATTCTCACATGTG+AGG + chr3_4:63653074-63653093 Msa0513420:CDS 35.0%
ATTCAAATTTGGAAGGCTTG+TGG - chr3_4:63653317-63653336 None:intergenic 35.0%
GAGAAGATAGAGGAGATAAT+TGG + chr3_4:63653358-63653377 Msa0513420:CDS 35.0%
! AGCTTTGACTGAAACATTTG+AGG - chr3_4:63653480-63653499 None:intergenic 35.0%
TTATACACTCCAGGTCTTTA+TGG + chr3_4:63653589-63653608 Msa0513420:CDS 35.0%
ACAGAAATTCCATAAAGACC+TGG - chr3_4:63653601-63653620 None:intergenic 35.0%
CAAAGATGAAGATGTTAACG+AGG + chr3_4:63653639-63653658 Msa0513420:CDS 35.0%
TCAGCAGCAACTATATACAG+AGG + chr3_4:63651768-63651787 Msa0513420:CDS 40.0%
TTGGACCAAACAACATAACG+AGG + chr3_4:63651992-63652011 Msa0513420:CDS 40.0%
TGGACCAAACAACATAACGA+GGG + chr3_4:63651993-63652012 Msa0513420:CDS 40.0%
GTATCCCTCGTTATGTTGTT+TGG - chr3_4:63652000-63652019 None:intergenic 40.0%
TGATTGTGATTCACTAGTCG+AGG + chr3_4:63652121-63652140 Msa0513420:CDS 40.0%
! ATTCACTAGTCGAGGTGTTT+GGG + chr3_4:63652129-63652148 Msa0513420:CDS 40.0%
!! CTCCTGCAGTTGATAATGAA+TGG - chr3_4:63652189-63652208 None:intergenic 40.0%
! GAGTTTGCTCTCTATATCCA+TGG + chr3_4:63652322-63652341 Msa0513420:CDS 40.0%
AGTTGAGTCTTGCAACATGA+TGG + chr3_4:63652376-63652395 Msa0513420:CDS 40.0%
CCTCTTCAACAGTGCATAAA+TGG - chr3_4:63652541-63652560 None:intergenic 40.0%
CCATTTATGCACTGTTGAAG+AGG + chr3_4:63652538-63652557 Msa0513420:CDS 40.0%
GAACAAAAACACTTGAGCTC+TGG - chr3_4:63652638-63652657 None:intergenic 40.0%
!! GAGCTCAAGTGTTTTTGTTC+AGG + chr3_4:63652638-63652657 Msa0513420:CDS 40.0%
AGTGTACGACTACGATATCA+TGG + chr3_4:63652661-63652680 Msa0513420:CDS 40.0%
TGGGGAAAGGTTGTCATATT+TGG - chr3_4:63652707-63652726 None:intergenic 40.0%
AATATGACAACCTTTCCCCA+TGG + chr3_4:63652707-63652726 Msa0513420:CDS 40.0%
ACGTTGTATCTTTGTGTAGG+TGG + chr3_4:63652928-63652947 Msa0513420:intron 40.0%
GATATGGATGAAGAGCTTCT+TGG + chr3_4:63652977-63652996 Msa0513420:CDS 40.0%
TTCCAAAACTGAAGCACATC+TGG + chr3_4:63653194-63653213 Msa0513420:CDS 40.0%
! TTCCAGATGTGCTTCAGTTT+TGG - chr3_4:63653199-63653218 None:intergenic 40.0%
AAGCACATCTGGAAACATCA+TGG + chr3_4:63653205-63653224 Msa0513420:CDS 40.0%
CTTGTGGCTATTGAAACATC+CGG - chr3_4:63653301-63653320 None:intergenic 40.0%
!! TTGTTAGCCTTGTGTTGTTG+CGG - chr3_4:63653409-63653428 None:intergenic 40.0%
! TGACTGAAACATTTGAGGCT+AGG - chr3_4:63653475-63653494 None:intergenic 40.0%
TTGAGATGCCACGATGTTAT+AGG + chr3_4:63653515-63653534 Msa0513420:CDS 40.0%
CCCAAAAGGTCTTCATCTTT+GGG - chr3_4:63653558-63653577 None:intergenic 40.0%
! CCCAAAGATGAAGACCTTTT+GGG + chr3_4:63653555-63653574 Msa0513420:CDS 40.0%
TCCCAAAAGGTCTTCATCTT+TGG - chr3_4:63653559-63653578 None:intergenic 40.0%
TAAGATTCCTTCGTCCCAAA+AGG - chr3_4:63653572-63653591 None:intergenic 40.0%
GAAGGAATCTTATACACTCC+AGG + chr3_4:63653580-63653599 Msa0513420:CDS 40.0%
GATGTTAACGAGGTGATACA+AGG + chr3_4:63653649-63653668 Msa0513420:CDS 40.0%
TCAGCAGCAACTATATACAG+AGG + chr3_4:63651768-63651787 Msa0513420:CDS 40.0%
TTGGACCAAACAACATAACG+AGG + chr3_4:63651992-63652011 Msa0513420:CDS 40.0%
TGGACCAAACAACATAACGA+GGG + chr3_4:63651993-63652012 Msa0513420:CDS 40.0%
GTATCCCTCGTTATGTTGTT+TGG - chr3_4:63652000-63652019 None:intergenic 40.0%
TGATTGTGATTCACTAGTCG+AGG + chr3_4:63652121-63652140 Msa0513420:CDS 40.0%
! ATTCACTAGTCGAGGTGTTT+GGG + chr3_4:63652129-63652148 Msa0513420:CDS 40.0%
!! CTCCTGCAGTTGATAATGAA+TGG - chr3_4:63652189-63652208 None:intergenic 40.0%
! GAGTTTGCTCTCTATATCCA+TGG + chr3_4:63652322-63652341 Msa0513420:CDS 40.0%
AGTTGAGTCTTGCAACATGA+TGG + chr3_4:63652376-63652395 Msa0513420:CDS 40.0%
CCTCTTCAACAGTGCATAAA+TGG - chr3_4:63652541-63652560 None:intergenic 40.0%
CCATTTATGCACTGTTGAAG+AGG + chr3_4:63652538-63652557 Msa0513420:CDS 40.0%
GAACAAAAACACTTGAGCTC+TGG - chr3_4:63652638-63652657 None:intergenic 40.0%
!! GAGCTCAAGTGTTTTTGTTC+AGG + chr3_4:63652638-63652657 Msa0513420:CDS 40.0%
AGTGTACGACTACGATATCA+TGG + chr3_4:63652661-63652680 Msa0513420:CDS 40.0%
TGGGGAAAGGTTGTCATATT+TGG - chr3_4:63652707-63652726 None:intergenic 40.0%
AATATGACAACCTTTCCCCA+TGG + chr3_4:63652707-63652726 Msa0513420:CDS 40.0%
ACGTTGTATCTTTGTGTAGG+TGG + chr3_4:63652928-63652947 Msa0513420:intron 40.0%
GATATGGATGAAGAGCTTCT+TGG + chr3_4:63652977-63652996 Msa0513420:CDS 40.0%
TTCCAAAACTGAAGCACATC+TGG + chr3_4:63653194-63653213 Msa0513420:CDS 40.0%
! TTCCAGATGTGCTTCAGTTT+TGG - chr3_4:63653199-63653218 None:intergenic 40.0%
AAGCACATCTGGAAACATCA+TGG + chr3_4:63653205-63653224 Msa0513420:CDS 40.0%
CTTGTGGCTATTGAAACATC+CGG - chr3_4:63653301-63653320 None:intergenic 40.0%
!! TTGTTAGCCTTGTGTTGTTG+CGG - chr3_4:63653409-63653428 None:intergenic 40.0%
! TGACTGAAACATTTGAGGCT+AGG - chr3_4:63653475-63653494 None:intergenic 40.0%
TTGAGATGCCACGATGTTAT+AGG + chr3_4:63653515-63653534 Msa0513420:CDS 40.0%
CCCAAAAGGTCTTCATCTTT+GGG - chr3_4:63653558-63653577 None:intergenic 40.0%
! CCCAAAGATGAAGACCTTTT+GGG + chr3_4:63653555-63653574 Msa0513420:CDS 40.0%
TCCCAAAAGGTCTTCATCTT+TGG - chr3_4:63653559-63653578 None:intergenic 40.0%
TAAGATTCCTTCGTCCCAAA+AGG - chr3_4:63653572-63653591 None:intergenic 40.0%
GAAGGAATCTTATACACTCC+AGG + chr3_4:63653580-63653599 Msa0513420:CDS 40.0%
GATGTTAACGAGGTGATACA+AGG + chr3_4:63653649-63653668 Msa0513420:CDS 40.0%
TGGAAGAAGGCCTTAAGGTT+GGG - chr3_4:63651617-63651636 None:intergenic 45.0%
TTGGAAGAAGGCCTTAAGGT+TGG - chr3_4:63651618-63651637 None:intergenic 45.0%
AACCTTAAGGCCTTCTTCCA+AGG + chr3_4:63651617-63651636 Msa0513420:five_prime_UTR 45.0%
! GTTTTACAGGTGGCATCACA+AGG + chr3_4:63651954-63651973 Msa0513420:intron 45.0%
! GATTCACTAGTCGAGGTGTT+TGG + chr3_4:63652128-63652147 Msa0513420:CDS 45.0%
!! TTGCACAAGGATTTTGGCCA+TGG - chr3_4:63652342-63652361 None:intergenic 45.0%
AGTGACAATTCCTCCAGCTT+AGG - chr3_4:63652464-63652483 None:intergenic 45.0%
ATGCACTGTTGAAGAGGACA+AGG + chr3_4:63652544-63652563 Msa0513420:CDS 45.0%
CACCCAACCTTCATCATCTA+CGG + chr3_4:63652747-63652766 Msa0513420:CDS 45.0%
CACCGTAGATGATGAAGGTT+GGG - chr3_4:63652752-63652771 None:intergenic 45.0%
CAACCTTCATCATCTACGGT+GGG + chr3_4:63652751-63652770 Msa0513420:CDS 45.0%
! ACATGTGAGGTATCTCACAG+TGG + chr3_4:63653087-63653106 Msa0513420:CDS 45.0%
! CATGTGAGGTATCTCACAGT+GGG + chr3_4:63653088-63653107 Msa0513420:CDS 45.0%
GCATGTTGCAGTGTGAACTA+TGG + chr3_4:63653152-63653171 Msa0513420:CDS 45.0%
GTCTGAATGCGAGAAGATAG+AGG + chr3_4:63653348-63653367 Msa0513420:CDS 45.0%
TGTTGAACCGCAACAACACA+AGG + chr3_4:63653399-63653418 Msa0513420:CDS 45.0%
GTGGCATCTCAACATAGGAA+GGG - chr3_4:63653507-63653526 None:intergenic 45.0%
ACATCGTGGCATCTCAACAT+AGG - chr3_4:63653512-63653531 None:intergenic 45.0%
! GCCCAAAGATGAAGACCTTT+TGG + chr3_4:63653554-63653573 Msa0513420:CDS 45.0%
!!! ATGAAGACCTTTTGGGACGA+AGG + chr3_4:63653562-63653581 Msa0513420:CDS 45.0%
TGGAAGAAGGCCTTAAGGTT+GGG - chr3_4:63651617-63651636 None:intergenic 45.0%
TTGGAAGAAGGCCTTAAGGT+TGG - chr3_4:63651618-63651637 None:intergenic 45.0%
AACCTTAAGGCCTTCTTCCA+AGG + chr3_4:63651617-63651636 Msa0513420:five_prime_UTR 45.0%
! GTTTTACAGGTGGCATCACA+AGG + chr3_4:63651954-63651973 Msa0513420:intron 45.0%
! GATTCACTAGTCGAGGTGTT+TGG + chr3_4:63652128-63652147 Msa0513420:CDS 45.0%
!! TTGCACAAGGATTTTGGCCA+TGG - chr3_4:63652342-63652361 None:intergenic 45.0%
AGTGACAATTCCTCCAGCTT+AGG - chr3_4:63652464-63652483 None:intergenic 45.0%
ATGCACTGTTGAAGAGGACA+AGG + chr3_4:63652544-63652563 Msa0513420:CDS 45.0%
CACCCAACCTTCATCATCTA+CGG + chr3_4:63652747-63652766 Msa0513420:CDS 45.0%
CACCGTAGATGATGAAGGTT+GGG - chr3_4:63652752-63652771 None:intergenic 45.0%
CAACCTTCATCATCTACGGT+GGG + chr3_4:63652751-63652770 Msa0513420:CDS 45.0%
! ACATGTGAGGTATCTCACAG+TGG + chr3_4:63653087-63653106 Msa0513420:CDS 45.0%
! CATGTGAGGTATCTCACAGT+GGG + chr3_4:63653088-63653107 Msa0513420:CDS 45.0%
GCATGTTGCAGTGTGAACTA+TGG + chr3_4:63653152-63653171 Msa0513420:CDS 45.0%
GTCTGAATGCGAGAAGATAG+AGG + chr3_4:63653348-63653367 Msa0513420:CDS 45.0%
TGTTGAACCGCAACAACACA+AGG + chr3_4:63653399-63653418 Msa0513420:CDS 45.0%
GTGGCATCTCAACATAGGAA+GGG - chr3_4:63653507-63653526 None:intergenic 45.0%
ACATCGTGGCATCTCAACAT+AGG - chr3_4:63653512-63653531 None:intergenic 45.0%
! GCCCAAAGATGAAGACCTTT+TGG + chr3_4:63653554-63653573 Msa0513420:CDS 45.0%
!!! ATGAAGACCTTTTGGGACGA+AGG + chr3_4:63653562-63653581 Msa0513420:CDS 45.0%
GACCTTGGAAGAAGGCCTTA+AGG - chr3_4:63651622-63651641 None:intergenic 50.0%
TTCTTACACCCCCATGCTTG+AGG + chr3_4:63651721-63651740 Msa0513420:exon 50.0%
ACGGTGAGATCCTCAAGCAT+GGG - chr3_4:63651734-63651753 None:intergenic 50.0%
TACGGTGAGATCCTCAAGCA+TGG - chr3_4:63651735-63651754 None:intergenic 50.0%
ACAGGTGGCATCACAAGGAT+TGG + chr3_4:63651959-63651978 Msa0513420:intron 50.0%
CCACCGTAGATGATGAAGGT+TGG - chr3_4:63652753-63652772 None:intergenic 50.0%
CCAACCTTCATCATCTACGG+TGG + chr3_4:63652750-63652769 Msa0513420:CDS 50.0%
ACTCCCACCGTAGATGATGA+AGG - chr3_4:63652757-63652776 None:intergenic 50.0%
TTCATCATCTACGGTGGGAG+TGG + chr3_4:63652756-63652775 Msa0513420:CDS 50.0%
TCATCATCTACGGTGGGAGT+GGG + chr3_4:63652757-63652776 Msa0513420:CDS 50.0%
CGTGGCATCTCAACATAGGA+AGG - chr3_4:63653508-63653527 None:intergenic 50.0%
GACCTTGGAAGAAGGCCTTA+AGG - chr3_4:63651622-63651641 None:intergenic 50.0%
TTCTTACACCCCCATGCTTG+AGG + chr3_4:63651721-63651740 Msa0513420:exon 50.0%
ACGGTGAGATCCTCAAGCAT+GGG - chr3_4:63651734-63651753 None:intergenic 50.0%
TACGGTGAGATCCTCAAGCA+TGG - chr3_4:63651735-63651754 None:intergenic 50.0%
ACAGGTGGCATCACAAGGAT+TGG + chr3_4:63651959-63651978 Msa0513420:intron 50.0%
CCACCGTAGATGATGAAGGT+TGG - chr3_4:63652753-63652772 None:intergenic 50.0%
CCAACCTTCATCATCTACGG+TGG + chr3_4:63652750-63652769 Msa0513420:CDS 50.0%
ACTCCCACCGTAGATGATGA+AGG - chr3_4:63652757-63652776 None:intergenic 50.0%
TTCATCATCTACGGTGGGAG+TGG + chr3_4:63652756-63652775 Msa0513420:CDS 50.0%
TCATCATCTACGGTGGGAGT+GGG + chr3_4:63652757-63652776 Msa0513420:CDS 50.0%
CGTGGCATCTCAACATAGGA+AGG - chr3_4:63653508-63653527 None:intergenic 50.0%
GGTGAGATCCTCAAGCATGG+GGG - chr3_4:63651732-63651751 None:intergenic 55.0%
CGGTGAGATCCTCAAGCATG+GGG - chr3_4:63651733-63651752 None:intergenic 55.0%
! CTAGTCGAGGTGTTTGGGTC+AGG + chr3_4:63652134-63652153 Msa0513420:CDS 55.0%
GTGTTTGGGTCAGGAGGAGA+AGG + chr3_4:63652143-63652162 Msa0513420:CDS 55.0%
CATCATCTACGGTGGGAGTG+GGG + chr3_4:63652758-63652777 Msa0513420:CDS 55.0%
GGTGAGATCCTCAAGCATGG+GGG - chr3_4:63651732-63651751 None:intergenic 55.0%
CGGTGAGATCCTCAAGCATG+GGG - chr3_4:63651733-63651752 None:intergenic 55.0%
! CTAGTCGAGGTGTTTGGGTC+AGG + chr3_4:63652134-63652153 Msa0513420:CDS 55.0%
GTGTTTGGGTCAGGAGGAGA+AGG + chr3_4:63652143-63652162 Msa0513420:CDS 55.0%
CATCATCTACGGTGGGAGTG+GGG + chr3_4:63652758-63652777 Msa0513420:CDS 55.0%
! GTCGAGGTGTTTGGGTCAGG+AGG + chr3_4:63652137-63652156 Msa0513420:CDS 60.0%
! GTCGAGGTGTTTGGGTCAGG+AGG + chr3_4:63652137-63652156 Msa0513420:CDS 60.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 63651613 63653684 63651613 ID=Msa0513420;Name=Msa0513420
chr3_4 mRNA 63651613 63653684 63651613 ID=Msa0513420-mRNA-1;Parent=Msa0513420;Name=Msa0513420-mRNA-1;_AED=0.35;_eAED=0.35;_QI=121|1|0.33|1|1|1|3|0|572
chr3_4 exon 63651613 63651829 63651613 ID=Msa0513420-mRNA-1:exon:15936;Parent=Msa0513420-mRNA-1
chr3_4 exon 63651963 63652847 63651963 ID=Msa0513420-mRNA-1:exon:15937;Parent=Msa0513420-mRNA-1
chr3_4 exon 63652947 63653684 63652947 ID=Msa0513420-mRNA-1:exon:15938;Parent=Msa0513420-mRNA-1
chr3_4 five_prime_UTR 63651613 63651733 63651613 ID=Msa0513420-mRNA-1:five_prime_utr;Parent=Msa0513420-mRNA-1
chr3_4 CDS 63651734 63651829 63651734 ID=Msa0513420-mRNA-1:cds;Parent=Msa0513420-mRNA-1
chr3_4 CDS 63651963 63652847 63651963 ID=Msa0513420-mRNA-1:cds;Parent=Msa0513420-mRNA-1
chr3_4 CDS 63652947 63653684 63652947 ID=Msa0513420-mRNA-1:cds;Parent=Msa0513420-mRNA-1
Gene Sequence

>Msa0513420

ATGCTTGAGGATCTCACCGTAAACATTAAATCACTCAGCAGCAACTATATACAGAGGAAAGACATAAATTCCGTTATACGGGGGTTTAAATCATTTGTGGCATCACAAGGATTGGAGATGTTGAATTGGACCAAACAACATAACGAGGGATACTTCATAGAAAACTCAAAAATAAGCATCAAAGAATTTCATAAATTGTTGGTACTTGTACCTGAGATACATATGCTTCAACATGTCATAGAACTCACTTTAAGTGATTGTGATTCACTAGTCGAGGTGTTTGGGTCAGGAGGAGAAGGTAGAAAAGAATACGATGGTACCATTCATTATCAACTGCAGGAGTTGAATTTGAACCGTTTGTCAAAATTGTGTCACATATGGAAACATAATATTACCAAGGTTGTAAGCTTCCAAAATCTAAAAGATATTAGTGTTTATTGTTGCCATAATTTGAAGAGTTTGCTCTCTATATCCATGGCCAAAATCCTTGTGCAACTTCAAAAACTACTAGTTGAGTCTTGCAACATGATGGATGAGATAACTACAAAGGAAGATGAAAATATAAAAGAAGGCAACAAAGTGAAGATTTTATTTCCTAAGCTGGAGGAATTGTCACTTGTTAATCTTCCAAAGCTGAAATGTTTTTGTTCAGGAGATTATGACTATGATATCCATTTATGCACTGTTGAAGAGGACAAGGAATTCAATAACAATGATAAGGTTCAGATTTCATTTCCACAATTAAAGGAATTATGGCTTCGTGAAGTACCAGAGCTCAAGTGTTTTTGTTCAGGAGTGTACGACTACGATATCATGGTGTCGTCAACTAATGAGTGTCCAAATATGACAACCTTTCCCCATGGAAATGTTATTGTAAACACACCCAACCTTCATCATCTACGGTGGGAGTGGGGATATAAACAAACACTTGGAGACCTAAATTTGACAATATATTATCATCATAATTCAGAGAAATACAAGGTGGAGTTGCAAAAATTAGAGACGTTTAGAGATATGGATGAAGAGCTTCTTGGCTACATGAAAATAGTAAAAGATCTTGAGATTGTAAACTGTCACAAGCTATTGAATTGCATCCCATCCAACATGATGCAATTATTCTCACATGTGAGGTATCTCACAGTGGGAGAATGTGAGTGCTTAGAAGAGATATTTGAATCAAATGATAGCATGTTGCAGTGTGAACTATGGCTATTGCATTTGTCTTATCTTCCAAAACTGAAGCACATCTGGAAACATCATGGTCAGATTATAGGATTTGAGTGTTTAAAAAGGATAAGCATAAAGCAGTGCAATGATTTGAAATATGTGCTTCCGGATGTTTCAATAGCCACAAGCCTTCCAAATTTGAATTCGATTTATGTGTCTGAATGCGAGAAGATAGAGGAGATAATTGGGAATAATTATTGTAATCATGTTGAACCGCAACAACACAAGGCTAACAAGATCATATTCCCATCATTGCTGTATATAGAACTAGAGAAGCTTCCTAGCCTCAAATGTTTCAGTCAAAGCTCTTTCCCTTCCTATGTTGAGATGCCACGATGTTATAGGATAAGAATTGAAGACTGCCCAAAGATGAAGACCTTTTGGGACGAAGGAATCTTATACACTCCAGGTCTTTATGGAATTTCTGTAGAAAATACCGAATTTGACAAAGATGAAGATGTTAACGAGGTGATACAAGGACACAATAAATGA
Protein sequence

>Msa0513420

MLEDLTVNIKSLSSNYIQRKDINSVIRGFKSFVASQGLEMLNWTKQHNEGYFIENSKISIKEFHKLLVLVPEIHMLQHVIELTLSDCDSLVEVFGSGGEGRKEYDGTIHYQLQELNLNRLSKLCHIWKHNITKVVSFQNLKDISVYCCHNLKSLLSISMAKILVQLQKLLVESCNMMDEITTKEDENIKEGNKVKILFPKLEELSLVNLPKLKCFCSGDYDYDIHLCTVEEDKEFNNNDKVQISFPQLKELWLREVPELKCFCSGVYDYDIMVSSTNECPNMTTFPHGNVIVNTPNLHHLRWEWGYKQTLGDLNLTIYYHHNSEKYKVELQKLETFRDMDEELLGYMKIVKDLEIVNCHKLLNCIPSNMMQLFSHVRYLTVGECECLEEIFESNDSMLQCELWLLHLSYLPKLKHIWKHHGQIIGFECLKRISIKQCNDLKYVLPDVSIATSLPNLNSIYVSECEKIEEIIGNNYCNHVEPQQHKANKIIFPSLLYIELEKLPSLKCFSQSSFPSYVEMPRCYRIRIEDCPKMKTFWDEGILYTPGLYGISVENTEFDKDEDVNEVIQGHNK*