AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0516130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0516130 MtrunA17_Chr6g0453781 55.764 373 77 11 1 345 1 313 7.92e-97 296
Msa0516130 MtrunA17_Chr7g0222301 53.482 359 135 13 1 350 1 336 5.93e-89 275
Msa0516130 MtrunA17_Chr7g0222241 52.646 359 141 12 1 353 1 336 1.61e-86 272
Msa0516130 MtrunA17_Chr7g0222281 52.646 359 141 12 1 353 1 336 1.61e-86 272
Msa0516130 MtrunA17_Chr5g0399271 50.519 289 114 7 112 378 1 282 3.06e-66 215
Msa0516130 MtrunA17_Chr5g0410641 79.762 84 17 0 390 473 381 464 1.80e-39 152
Msa0516130 MtrunA17_Chr4g0039801 73.810 84 22 0 390 473 402 485 1.30e-35 141
Msa0516130 MtrunA17_Chr1g0165421 73.810 84 22 0 390 473 388 471 5.10e-35 139
Msa0516130 MtrunA17_Chr1g0165411 71.429 84 24 0 390 473 374 457 1.83e-34 137
Msa0516130 MtrunA17_Chr2g0297351 63.218 87 32 0 387 473 285 371 1.18e-32 132
Msa0516130 MtrunA17_Chr7g0242471 52.597 154 64 6 45 197 1 146 2.25e-32 121
Msa0516130 MtrunA17_Chr7g0274751 67.857 84 27 0 390 473 225 308 8.24e-32 129
Msa0516130 MtrunA17_Chr2g0332991 61.458 96 37 0 4 99 7 102 6.46e-30 114
Msa0516130 MtrunA17_Chr3g0088821 57.143 84 36 0 390 473 379 462 1.26e-26 114
Msa0516130 MtrunA17_Chr7g0269741 65.476 84 29 0 390 473 426 509 2.73e-26 113
Msa0516130 MtrunA17_Chr3g0137951 55.952 84 37 0 390 473 389 472 5.65e-26 112
Msa0516130 MtrunA17_Chr7g0235851 30.909 275 164 7 45 312 1 256 1.12e-25 108
Msa0516130 MtrunA17_Chr7g0235821 30.545 275 165 7 45 312 1 256 1.72e-25 107
Msa0516130 MtrunA17_Chr8g0352231 55.952 84 37 0 390 473 379 462 1.86e-25 110
Msa0516130 MtrunA17_Chr8g0379241 54.762 84 38 0 390 473 304 387 2.27e-25 110
Msa0516130 MtrunA17_Chr4g0073711 57.143 84 36 0 390 473 359 442 4.04e-25 109
Msa0516130 MtrunA17_Chr1g0183381 53.488 86 40 0 390 475 231 316 4.80e-25 109
Msa0516130 MtrunA17_Chr8g0383701 53.571 84 39 0 390 473 397 480 1.07e-24 108
Msa0516130 MtrunA17_Chr2g0284211 53.571 84 39 0 390 473 289 372 3.58e-24 106
Msa0516130 MtrunA17_Chr7g0267731 52.381 84 40 0 390 473 291 374 1.29e-23 104
Msa0516130 MtrunA17_Chr3g0078671 53.571 84 39 0 390 473 346 429 1.93e-23 104
Msa0516130 MtrunA17_Chr8g0364641 52.381 84 40 0 390 473 313 396 1.93e-23 104
Msa0516130 MtrunA17_Chr1g0201731 50.000 84 42 0 390 473 399 482 5.10e-23 103
Msa0516130 MtrunA17_Chr2g0296991 51.190 84 41 0 390 473 330 413 1.25e-22 102
Msa0516130 MtrunA17_Chr6g0458601 32.852 277 153 9 49 312 64 320 1.84e-22 99.8
Msa0516130 MtrunA17_Chr3g0086041 30.769 273 164 9 48 315 25 277 1.61e-20 93.2
Msa0516130 MtrunA17_Chr5g0431711 30.657 274 162 9 49 315 11 263 5.38e-20 91.7
Msa0516130 MtrunA17_Chr7g0246351 29.595 321 189 11 1 318 1 287 6.24e-20 91.7
Msa0516130 MtrunA17_Chr3g0093361 29.032 279 177 9 30 299 24 290 3.65e-19 89.4
Msa0516130 MtrunA17_Chr5g0419431 29.245 318 178 11 51 350 16 304 3.67e-19 89.4
Msa0516130 MtrunA17_Chr1g0193401 31.071 280 156 10 49 311 179 438 5.58e-19 89.7
Msa0516130 MtrunA17_Chr5g0423851 32.593 270 154 10 49 311 8 256 6.94e-19 88.2
Msa0516130 MtrunA17_Chr3g0137721 29.044 272 170 7 49 311 11 268 1.56e-18 87.4
Msa0516130 MtrunA17_Chr7g0227851 28.065 310 192 9 44 344 6 293 1.73e-18 87.0
Msa0516130 MtrunA17_Chr1g0208801 30.588 255 146 7 46 289 5 239 7.31e-18 85.5
Msa0516130 MtrunA17_Chr3g0091171 29.123 285 159 8 45 318 1 253 1.36e-17 84.3
Msa0516130 MtrunA17_Chr3g0086141 33.654 208 117 7 48 246 2 197 1.89e-17 81.3
Msa0516130 MtrunA17_Chr5g0419701 31.452 248 142 9 51 289 6 234 2.21e-17 84.0
Msa0516130 MtrunA17_Chr7g0226481 27.746 346 151 13 3 345 8 257 4.89e-17 82.4
Msa0516130 MtrunA17_Chr2g0277281 25.994 327 204 11 48 358 10 314 1.35e-16 81.6
Msa0516130 MtrunA17_Chr3g0137681 27.972 286 174 10 47 318 8 275 2.39e-16 80.9
Msa0516130 MtrunA17_Chr6g0471031 31.600 250 147 8 47 289 59 291 4.48e-16 80.5
Msa0516130 MtrunA17_Chr1g0166251 27.619 315 194 12 44 348 6 296 5.67e-16 79.7
Msa0516130 MtrunA17_Chr7g0238361 26.974 304 173 12 49 328 3 281 9.79e-16 79.0
Msa0516130 MtrunA17_Chr7g0236211 28.197 305 189 8 49 344 12 295 1.26e-15 78.6
Msa0516130 MtrunA17_Chr3g0138371 29.499 339 191 14 47 371 18 322 1.49e-15 78.6
Msa0516130 MtrunA17_Chr7g0274231 27.386 241 153 6 44 270 7 239 1.80e-15 76.6
Msa0516130 MtrunA17_Chr3g0114151 28.214 280 164 8 47 320 22 270 2.68e-15 77.8
Msa0516130 MtrunA17_Chr7g0227771 29.798 198 116 4 49 238 4 186 3.05e-15 74.3
Msa0516130 MtrunA17_Chr1g0186381 41.489 94 51 2 390 479 132 225 3.72e-15 78.2
Msa0516130 MtrunA17_Chr2g0278121 26.452 310 165 10 47 311 14 305 5.04e-15 77.0
Msa0516130 MtrunA17_Chr2g0322341 41.176 85 49 1 390 474 126 209 5.71e-15 76.3
Msa0516130 MtrunA17_Chr3g0138571 29.643 280 173 8 38 311 2 263 8.38e-15 76.3
Msa0516130 MtrunA17_Chr2g0278101 26.136 264 164 8 49 294 12 262 1.28e-14 74.7
Msa0516130 MtrunA17_Chr5g0395311 27.714 350 200 13 51 373 28 351 1.82e-14 75.5
Msa0516130 MtrunA17_Chr7g0227651 26.220 328 195 10 44 355 6 302 1.83e-14 75.1
Msa0516130 MtrunA17_Chr2g0322351 38.824 85 52 0 390 474 122 206 2.46e-14 75.9
Msa0516130 MtrunA17_Chr7g0255101 28.758 306 189 8 49 348 110 392 2.74e-14 75.1
Msa0516130 MtrunA17_Chr5g0425131 29.921 254 148 9 49 293 11 243 3.48e-14 72.4
Msa0516130 MtrunA17_Chr3g0132631 28.521 284 136 11 48 309 11 249 3.74e-14 73.9
Msa0516130 MtrunA17_Chr1g0181601 28.866 291 151 9 48 311 7 268 4.68e-14 73.9
Msa0516130 MtrunA17_Chr4g0023251 27.823 248 137 11 49 273 2 230 5.31e-14 72.8
Msa0516130 MtrunA17_Chr6g0466481 29.562 274 163 9 47 311 6 258 5.44e-14 73.6
Msa0516130 MtrunA17_Chr6g0465671 29.562 274 163 9 47 311 6 258 6.89e-14 73.2
Msa0516130 MtrunA17_Chr5g0423901 26.879 346 212 13 48 380 8 325 7.55e-14 73.2
Msa0516130 MtrunA17_Chr5g0419561 28.261 276 167 8 48 311 11 267 1.32e-13 72.4
Msa0516130 MtrunA17_Chr7g0224001 32.161 199 122 6 46 238 11 202 1.53e-13 72.4
Msa0516130 MtrunA17_Chr7g0235361 28.261 276 169 8 48 312 39 296 2.16e-13 71.6
Msa0516130 MtrunA17_Chr6g0458501 29.321 324 174 13 48 354 42 327 2.35e-13 71.6
Msa0516130 MtrunA17_Chr7g0235431 29.450 309 182 11 40 344 4 280 4.44e-13 70.9
Msa0516130 MtrunA17_Chr2g0278791 27.744 328 175 12 49 353 2 290 9.47e-13 69.7
Msa0516130 MtrunA17_Chr3g0102531 40.000 85 45 3 390 474 126 204 1.24e-12 70.5
Msa0516130 MtrunA17_Chr3g0137701 26.370 292 178 9 49 319 9 284 1.25e-12 69.3
Msa0516130 MtrunA17_Chr7g0262081 29.242 277 162 9 47 313 28 280 1.50e-12 69.3
Msa0516130 MtrunA17_Chr6g0465731 29.756 205 129 5 47 248 6 198 1.63e-12 68.9
Msa0516130 MtrunA17_Chr2g0322361 53.846 52 24 0 390 441 127 178 1.66e-12 70.1
Msa0516130 MtrunA17_Chr5g0438051 28.472 288 171 11 46 309 3 279 2.04e-12 68.9
Msa0516130 MtrunA17_Chr3g0107721 25.843 356 225 13 48 383 2 338 2.38e-12 68.6
Msa0516130 MtrunA17_Chr2g0322371 51.923 52 25 0 390 441 120 171 2.77e-12 69.3
Msa0516130 MtrunA17_Chr3g0102551 38.824 85 45 1 390 474 136 213 2.83e-12 69.3
Msa0516130 MtrunA17_Chr7g0262091 28.986 276 166 8 47 313 16 270 2.85e-12 68.2
Msa0516130 MtrunA17_Chr5g0396411 24.919 309 181 11 51 345 1 272 3.69e-12 68.2
Msa0516130 MtrunA17_Chr3g0136291 28.713 202 132 4 42 242 7 197 4.05e-12 67.4
Msa0516130 MtrunA17_Chr2g0278801 28.405 257 156 7 49 293 2 242 7.00e-12 65.5
Msa0516130 MtrunA17_Chr8g0378391 61.224 49 19 0 1 49 6 54 8.24e-12 60.8
Msa0516130 MtrunA17_Chr7g0236221 59.574 47 19 0 3 49 6 52 2.35e-11 61.2
Msa0516130 MtrunA17_Chr3g0102541 37.647 85 48 1 390 474 126 205 2.69e-11 66.2
Msa0516130 MtrunA17_Chr2g0307191 35.714 84 54 0 390 473 118 201 3.08e-11 65.9
Msa0516130 MtrunA17_Chr5g0420741 30.000 250 147 7 47 289 16 244 3.36e-11 63.9
Msa0516130 MtrunA17_Chr4g0046401 36.047 86 54 1 390 474 131 216 3.47e-11 65.9
Msa0516130 MtrunA17_Chr2g0298581 36.047 86 53 2 390 474 118 202 4.52e-11 65.5
Msa0516130 MtrunA17_Chr5g0416541 29.435 248 155 7 48 289 19 252 5.10e-11 64.3
Msa0516130 MtrunA17_Chr3g0095791 37.234 94 55 3 390 481 118 209 6.62e-11 64.7
Msa0516130 MtrunA17_Chr7g0262121 25.166 302 200 8 48 344 19 299 7.10e-11 63.9
Msa0516130 MtrunA17_Chr0c09g0493551 28.571 210 135 5 47 253 6 203 8.08e-11 63.2
Msa0516130 MtrunA17_Chr3g0091151 51.351 74 34 1 6 77 7 80 9.87e-11 58.2
Msa0516130 MtrunA17_Chr7g0227621 70.000 40 12 0 3 42 8 47 9.91e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0516130 AT3G03050.1 73.810 84 22 0 390 473 407 490 9.75e-37 145
Msa0516130 AT5G16910.1 73.810 84 22 0 390 473 410 493 1.11e-36 144
Msa0516130 AT4G38190.1 72.619 84 23 0 390 473 385 468 6.97e-35 139
Msa0516130 AT1G32180.1 67.857 84 27 0 390 473 235 318 1.03e-32 132
Msa0516130 AT1G32180.2 67.857 84 27 0 390 473 235 318 1.03e-32 132
Msa0516130 AT2G33100.2 65.476 84 29 0 390 473 296 379 3.35e-32 131
Msa0516130 AT2G33100.1 65.476 84 29 0 390 473 296 379 3.35e-32 131
Msa0516130 AT5G05170.1 54.762 84 38 0 390 473 367 450 1.09e-25 111
Msa0516130 AT2G25540.1 54.651 86 39 0 390 475 363 448 1.24e-25 111
Msa0516130 AT1G02730.1 64.286 84 30 0 390 473 431 514 1.97e-25 110
Msa0516130 AT5G17420.1 54.023 87 40 0 390 476 346 432 2.46e-25 110
Msa0516130 AT4G32410.1 53.571 84 39 0 390 473 383 466 1.81e-24 107
Msa0516130 AT5G09870.1 53.571 84 39 0 390 473 372 455 2.32e-24 107
Msa0516130 AT4G18780.1 51.190 84 41 0 390 473 292 375 2.88e-24 107
Msa0516130 AT4G39350.1 53.571 84 39 0 390 473 385 468 4.67e-24 106
Msa0516130 AT5G44030.3 53.571 84 39 0 390 473 44 127 6.57e-24 105
Msa0516130 AT5G64740.1 53.571 84 39 0 390 473 384 467 6.76e-24 106
Msa0516130 AT5G44030.1 53.571 84 39 0 390 473 323 406 1.29e-23 105
Msa0516130 AT5G44030.2 53.571 84 39 0 390 473 352 435 1.37e-23 105
Msa0516130 AT2G21770.1 51.190 84 41 0 390 473 390 473 6.32e-22 100
Msa0516130 AT4G14103.3 30.198 202 124 5 49 244 8 198 3.39e-15 77.8
Msa0516130 AT4G14103.1 30.198 202 124 5 49 244 8 198 3.39e-15 77.8
Msa0516130 AT4G14103.2 30.198 202 124 5 49 244 8 198 4.94e-15 77.4
Msa0516130 AT5G22670.1 31.188 202 121 5 49 249 11 195 1.46e-14 76.3
Msa0516130 AT2G32530.1 40.000 85 48 1 390 474 124 205 6.95e-14 74.7
Msa0516130 AT4G24010.1 43.529 85 40 2 390 474 130 206 1.75e-13 73.6
Msa0516130 AT2G32540.1 37.647 85 50 1 390 474 124 205 3.22e-13 72.8
Msa0516130 AT4G23990.1 43.529 85 40 2 390 474 149 225 4.36e-13 72.4
Msa0516130 AT3G59200.2 27.317 205 126 7 49 243 7 198 5.45e-13 70.9
Msa0516130 AT3G59200.1 27.317 205 126 7 49 243 7 198 6.35e-13 71.2
Msa0516130 AT2G32620.4 36.471 85 51 1 390 474 28 109 1.10e-12 70.9
Msa0516130 AT2G32620.3 36.471 85 51 1 390 474 124 205 1.18e-12 70.5
Msa0516130 AT2G32620.1 36.471 85 51 1 390 474 124 205 1.22e-12 70.9
Msa0516130 AT4G00160.1 28.571 231 151 7 40 269 8 225 1.49e-12 70.1
Msa0516130 AT2G32620.2 36.471 85 51 1 390 474 124 205 1.51e-12 70.1
Msa0516130 AT1G78750.2 29.167 216 121 6 47 247 16 214 1.54e-12 70.1
Msa0516130 AT1G78750.1 29.167 216 121 6 47 247 16 214 1.54e-12 70.1
Msa0516130 AT3G59190.2 29.064 203 122 7 49 240 12 203 1.57e-12 70.1
Msa0516130 AT3G59190.1 28.708 209 127 7 49 246 12 209 1.58e-12 69.3
Msa0516130 AT3G58860.1 29.907 214 127 10 47 250 5 205 3.44e-12 68.9
Msa0516130 AT4G14096.2 27.723 202 125 8 49 243 8 195 4.58e-12 68.6
Msa0516130 AT4G14096.1 27.723 202 125 8 49 243 8 195 4.58e-12 68.6
Msa0516130 AT5G60610.2 28.037 214 137 4 49 262 2 198 5.60e-12 67.8
Msa0516130 AT5G60610.1 28.037 214 137 4 49 262 2 198 5.60e-12 67.8
Msa0516130 AT2G32610.1 36.047 86 51 2 390 474 124 206 6.14e-12 68.6
Msa0516130 AT2G42730.1 25.941 239 141 9 45 267 1 219 1.04e-11 67.4
Msa0516130 AT4G15290.1 40.260 77 43 1 390 466 124 197 1.91e-11 67.0
Msa0516130 AT4G15320.5 40.260 77 43 1 390 466 124 197 1.96e-11 67.0
Msa0516130 AT4G15320.3 40.260 77 43 1 390 466 124 197 2.01e-11 67.0
Msa0516130 AT5G56690.1 29.665 209 135 5 51 255 4 204 2.13e-11 66.2
Msa0516130 AT4G15320.2 40.260 77 43 1 390 466 124 197 2.27e-11 66.6
Msa0516130 AT3G03040.1 28.780 205 126 7 49 243 2 196 3.44e-11 65.9
Msa0516130 AT4G10400.2 25.551 227 150 6 49 272 2 212 4.35e-11 65.1
Msa0516130 AT4G10400.3 25.551 227 150 6 49 272 2 212 4.35e-11 65.1
Msa0516130 AT4G10400.1 25.551 227 150 6 49 272 2 212 4.35e-11 65.1
Msa0516130 AT3G49150.1 27.488 211 131 9 47 248 14 211 4.55e-11 65.5
Msa0516130 AT5G41840.1 27.397 219 140 6 48 253 13 225 5.36e-11 65.5
Msa0516130 AT5G56370.3 27.619 210 141 5 49 258 2 200 5.92e-11 64.3
Msa0516130 AT4G09920.1 25.743 202 135 4 49 247 2 191 6.15e-11 63.9
Msa0516130 AT4G09920.5 25.743 202 135 4 49 247 2 191 6.15e-11 63.9
Msa0516130 AT4G15320.4 35.870 92 52 2 390 477 124 212 6.26e-11 65.5
Msa0516130 AT1G55850.1 38.824 85 44 2 390 474 140 216 6.46e-11 65.5
Msa0516130 AT5G56420.1 28.019 207 135 5 49 253 6 200 6.54e-11 64.7
Msa0516130 AT5G56420.2 28.019 207 135 5 49 253 6 200 6.54e-11 64.7
Msa0516130 AT5G56420.3 28.019 207 135 5 49 253 6 200 6.54e-11 64.7
Msa0516130 AT5G56370.1 27.619 210 141 5 49 258 2 200 7.06e-11 64.7
Msa0516130 AT5G56370.2 27.619 210 141 5 49 258 2 200 7.06e-11 64.7
Msa0516130 AT4G15320.1 35.870 92 52 2 390 477 124 212 7.09e-11 65.1
Msa0516130 AT3G59170.1 27.477 222 128 4 49 243 7 222 8.70e-11 64.3

Find 91 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTCTTGTGATTCATTATA+AGG 0.182897 3_4:-67569163 Msa0516130:intron
GGAATTCAAGGTTCGGATTA+AGG 0.237321 3_4:-67567740 Msa0516130:CDS
TGCATACTAAAATATGATTC+AGG 0.249233 3_4:+67567835 None:intergenic
ATTTCTCAATTTAACTCATA+TGG 0.278128 3_4:-67569277 Msa0516130:CDS
GCTTCAATTCACATAAATTA+TGG 0.282452 3_4:-67568952 Msa0516130:CDS
TTTGGTCACTAGAACATATA+AGG 0.297458 3_4:+67569838 None:intergenic
TGAACGTACTTTGTTCTTGC+AGG 0.304293 3_4:+67567804 None:intergenic
CAGTATGATCATTATAATAA+TGG 0.318351 3_4:-67567715 Msa0516130:CDS
GCGCTGATTACGTCTGCTTT+AGG 0.335866 3_4:+67570047 None:intergenic
ATGTGGGCTTTGAGCAAATT+GGG 0.352615 3_4:+67569405 None:intergenic
GTGATGAAAGACATTGCATT+TGG 0.355377 3_4:+67568988 None:intergenic
TTGCTGATTCTGTCTCTTGT+TGG 0.357008 3_4:+67570176 None:intergenic
AATGTGGGCTTTGAGCAAAT+TGG 0.359240 3_4:+67569404 None:intergenic
GAAAATGAACCCTTGTGAAA+TGG 0.363991 3_4:+67569547 None:intergenic
GAAGGTGCAGTATGAGTATC+AGG 0.370887 3_4:-67567761 Msa0516130:CDS
TGATGAAAGACATTGCATTT+GGG 0.375791 3_4:+67568989 None:intergenic
TATTTGTGAGGAGGGCAAAA+AGG 0.400440 3_4:+67570121 None:intergenic
ATCTTCTCATACAAGAGAAT+GGG 0.409440 3_4:+67569681 None:intergenic
AGACGTAATCAGCGCTTTAC+CGG 0.409516 3_4:-67570039 Msa0516130:CDS
ACATTGTAAAACAAGTTCAA+TGG 0.430783 3_4:+67569348 None:intergenic
CATTGTAAAACAAGTTCAAT+GGG 0.433991 3_4:+67569349 None:intergenic
TGATATCACGAATTCTTTCA+AGG 0.438997 3_4:+67569931 None:intergenic
GATGTCGGTATAATCAAAAT+AGG 0.441853 3_4:+67569869 None:intergenic
ACTCTTCTTCGTTAAGAGCT+TGG 0.442132 3_4:-67569773 Msa0516130:CDS
ATTGAAGCTCACACTTTCCT+TGG 0.446188 3_4:+67568927 None:intergenic
ATGTGATGAATTAATTCATC+TGG 0.448816 3_4:+67570149 None:intergenic
CAATGGGAAACAAGAAGAAA+TGG 0.451384 3_4:+67569365 None:intergenic
ATGGTGTGCTTCCGACAAAA+TGG 0.452233 3_4:+67567871 None:intergenic
TCTCCAGCGCCTTGAGAGAC+AGG 0.455545 3_4:+67569959 None:intergenic
AATCTTCTCATACAAGAGAA+TGG 0.464249 3_4:+67569680 None:intergenic
TAACAAGATAGTTTATCAAC+AGG 0.466967 3_4:+67567970 None:intergenic
GGCTTTGAGCAAATTGGGGA+AGG 0.472421 3_4:+67569410 None:intergenic
AGTACGTTCAGACTTTGTTA+AGG 0.472762 3_4:-67567791 Msa0516130:CDS
GCTAATAACTTTCGAGGCCA+TGG 0.479032 3_4:-67567926 Msa0516130:CDS
AATATGATTCAGGGCTCCTT+AGG 0.485055 3_4:+67567845 None:intergenic
CTCCGTATGTCCGCTTCTTG+AGG 0.485766 3_4:-67569496 Msa0516130:CDS
GCATACTAAAATATGATTCA+GGG 0.494986 3_4:+67567836 None:intergenic
AAGAGTGTGAAGAGAGAAGC+AGG 0.495864 3_4:+67569179 None:intergenic
CGACACGGAGGATGATCATC+GGG 0.502249 3_4:-67569901 Msa0516130:CDS
ATCCTCAAGAAGCGGACATA+CGG 0.512549 3_4:+67569494 None:intergenic
AGTATCAGGAATTCAAGGTT+CGG 0.515592 3_4:-67567747 Msa0516130:CDS
AACTTTCGAGGCCATGGCAG+AGG 0.517339 3_4:-67567920 Msa0516130:CDS
GTTATGTCTTTGACGACGGC+GGG 0.520325 3_4:-67567951 Msa0516130:CDS
TATCCTGTCTCTCAAGGCGC+TGG 0.525849 3_4:-67569962 Msa0516130:CDS
CAAAGCGATGGAATCGACTC+TGG 0.529050 3_4:-67570079 Msa0516130:CDS
GAACGTACTTTGTTCTTGCA+GGG 0.533706 3_4:+67567805 None:intergenic
TTCAGGGCTCCTTAGGTCAA+TGG 0.536107 3_4:+67567852 None:intergenic
TCTTCGTTAAGAGCTTGGCT+TGG 0.542922 3_4:-67569768 Msa0516130:CDS
TTGCAGTATCCTGTCTCTCA+AGG 0.547564 3_4:-67569968 Msa0516130:CDS
AACGAAGAAGAGTTGGCAAA+GGG 0.548836 3_4:+67569783 None:intergenic
TCTTGTTATGTCTTTGACGA+CGG 0.549146 3_4:-67567955 Msa0516130:CDS
CGGGTCGCTAATAACTTTCG+AGG 0.553897 3_4:-67567932 Msa0516130:CDS
CAAGTCTTCTCTCAAAGCGA+TGG 0.554198 3_4:-67570091 Msa0516130:CDS
AGCTCTTAACGAAGAAGAGT+TGG 0.554895 3_4:+67569776 None:intergenic
TATAAGGGCGCAGGCGATGT+CGG 0.559906 3_4:+67569854 None:intergenic
GGAAGAAGAGTCAGAAATGT+GGG 0.560085 3_4:+67569389 None:intergenic
CAGACTTTGTTAAGGATCGC+AGG 0.571470 3_4:-67567783 Msa0516130:CDS
TCACATAAATTATGGAGCCA+AGG 0.574469 3_4:-67568944 Msa0516130:CDS
GTGAGGGCTATTTGTGAGGA+GGG 0.574537 3_4:+67570113 None:intergenic
TTGGTCACTAGAACATATAA+GGG 0.580583 3_4:+67569839 None:intergenic
AGGCTGCTGTGGCGAAGAAG+TGG 0.580903 3_4:-67569035 Msa0516130:CDS
TTTAATTGTTGTCAGTTCGG+AGG 0.581299 3_4:-67569055 Msa0516130:intron
TCATATGGAGCTTACAATTG+TGG 0.582407 3_4:-67569262 Msa0516130:CDS
AAAACTCGCTGCCTCTGCCA+TGG 0.590802 3_4:+67567909 None:intergenic
ACTAGAACATATAAGGGCGC+AGG 0.591034 3_4:+67569845 None:intergenic
TGTGAGGGCTATTTGTGAGG+AGG 0.593007 3_4:+67570112 None:intergenic
CTTTGAGAGAAGACTTGTGA+GGG 0.596047 3_4:+67570097 None:intergenic
CTTTGTTAAGGATCGCAGGA+AGG 0.596094 3_4:-67567779 Msa0516130:CDS
GTATGAGTATCAGGAATTCA+AGG 0.596509 3_4:-67567752 Msa0516130:CDS
ATGAGTAAATCCTCAAGAAG+CGG 0.596627 3_4:+67569486 None:intergenic
TGTCAGTTCGGAGGCTGCTG+TGG 0.605598 3_4:-67569046 Msa0516130:intron
GCTTTGAGCAAATTGGGGAA+GGG 0.606125 3_4:+67569411 None:intergenic
ACGACACGGAGGATGATCAT+CGG 0.606432 3_4:-67569902 Msa0516130:CDS
ATGCGACATAGTAGTTCGAC+CGG 0.608059 3_4:+67570020 None:intergenic
TTTGAACTTGAATGGGAATG+GGG 0.608159 3_4:-67569634 Msa0516130:CDS
TAACGAAGAAGAGTTGGCAA+AGG 0.609423 3_4:+67569782 None:intergenic
TGTTATGTCTTTGACGACGG+CGG 0.610637 3_4:-67567952 Msa0516130:CDS
ATGCAATGTCTTTCATCACA+AGG 0.621682 3_4:-67568984 Msa0516130:CDS
GGAAGCACACCATTGACCTA+AGG 0.622518 3_4:-67567861 Msa0516130:CDS
TCTTCTCATACAAGAGAATG+GGG 0.625256 3_4:+67569682 None:intergenic
GATGAAAGACATTGCATTTG+GGG 0.625948 3_4:+67568990 None:intergenic
TGGGAAACAAGAAGAAATGG+AGG 0.628867 3_4:+67569368 None:intergenic
ACTTGTGAGGGCTATTTGTG+AGG 0.630738 3_4:+67570109 None:intergenic
TGTGGGCTTTGAGCAAATTG+GGG 0.639278 3_4:+67569406 None:intergenic
AGGAAGAAGAGTCAGAAATG+TGG 0.643929 3_4:+67569388 None:intergenic
GCTTTGAGAGAAGACTTGTG+AGG 0.657687 3_4:+67570096 None:intergenic
ATGAAAGACATTGCATTTGG+GGG 0.717377 3_4:+67568991 None:intergenic
TGATATCAACTTGAACGACA+CGG 0.722529 3_4:-67569916 Msa0516130:CDS
CTTCTCATACAAGAGAATGG+GGG 0.736891 3_4:+67569683 None:intergenic
AGTGTGAAGAGAGAAGCAGG+CGG 0.753172 3_4:+67569182 None:intergenic
TATCAACTTGAACGACACGG+AGG 0.778510 3_4:-67569913 Msa0516130:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTCTCAATTTAACTCATA+TGG - chr3_4:67568613-67568632 Msa0516130:intron 20.0%
!! TTATTTAATTGTTGTCAGTT+CGG - chr3_4:67568832-67568851 Msa0516130:intron 20.0%
!! CAGTATGATCATTATAATAA+TGG - chr3_4:67570175-67570194 Msa0516130:CDS 20.0%
! ATGTGATGAATTAATTCATC+TGG + chr3_4:67567744-67567763 None:intergenic 25.0%
!!! CAAAAGAGTTAAAGTTTTGA+GGG + chr3_4:67568078-67568097 None:intergenic 25.0%
!! ACAAAAGAGTTAAAGTTTTG+AGG + chr3_4:67568079-67568098 None:intergenic 25.0%
!!! TTGTTGTTTTGAACTTGAAT+GGG - chr3_4:67568249-67568268 Msa0516130:intron 25.0%
! CATTGTAAAACAAGTTCAAT+GGG + chr3_4:67568544-67568563 None:intergenic 25.0%
! ACATTGTAAAACAAGTTCAA+TGG + chr3_4:67568545-67568564 None:intergenic 25.0%
! AACAAAGATAAAACAACGAA+TGG + chr3_4:67568780-67568799 None:intergenic 25.0%
! GCTTCAATTCACATAAATTA+TGG - chr3_4:67568938-67568957 Msa0516130:intron 25.0%
!! CTAATTGTTTGATAATCTTC+GGG + chr3_4:67569019-67569038 None:intergenic 25.0%
!! CCTAATTGTTTGATAATCTT+CGG + chr3_4:67569020-67569039 None:intergenic 25.0%
! CGAAGATTATCAAACAATTA+GGG - chr3_4:67569018-67569037 Msa0516130:CDS 25.0%
! GAAGATTATCAAACAATTAG+GGG - chr3_4:67569019-67569038 Msa0516130:CDS 25.0%
!!! TTTTCGCTGCTTTTATTTAT+TGG - chr3_4:67569095-67569114 Msa0516130:intron 25.0%
!!! TCTCTTCAAAGTTTTGTTTT+TGG - chr3_4:67569512-67569531 Msa0516130:CDS 25.0%
!!! CTCTTCAAAGTTTTGTTTTT+GGG - chr3_4:67569513-67569532 Msa0516130:CDS 25.0%
!! CAAGAATCTTTTATAAGCAT+TGG + chr3_4:67569739-67569758 None:intergenic 25.0%
! CAATGCTTATAAAAGATTCT+TGG - chr3_4:67569737-67569756 Msa0516130:CDS 25.0%
! TAACAAGATAGTTTATCAAC+AGG + chr3_4:67569923-67569942 None:intergenic 25.0%
! GCATACTAAAATATGATTCA+GGG + chr3_4:67570057-67570076 None:intergenic 25.0%
! TGCATACTAAAATATGATTC+AGG + chr3_4:67570058-67570077 None:intergenic 25.0%
!!! TACTGCAAAGATTTGTTTTG+CGG + chr3_4:67567910-67567929 None:intergenic 30.0%
TGATATCACGAATTCTTTCA+AGG + chr3_4:67567962-67567981 None:intergenic 30.0%
GATGTCGGTATAATCAAAAT+AGG + chr3_4:67568024-67568043 None:intergenic 30.0%
TTGGTCACTAGAACATATAA+GGG + chr3_4:67568054-67568073 None:intergenic 30.0%
TTTGGTCACTAGAACATATA+AGG + chr3_4:67568055-67568074 None:intergenic 30.0%
ATCTTCTCATACAAGAGAAT+GGG + chr3_4:67568212-67568231 None:intergenic 30.0%
AATCTTCTCATACAAGAGAA+TGG + chr3_4:67568213-67568232 None:intergenic 30.0%
!!! CTTGTTGTTTTGAACTTGAA+TGG - chr3_4:67568248-67568267 Msa0516130:intron 30.0%
! TTTTGAACTTGAATGGGAAT+GGG - chr3_4:67568255-67568274 Msa0516130:intron 30.0%
!!! AAATGGAGTTTTTTGAGGTT+TGG + chr3_4:67568329-67568348 None:intergenic 30.0%
!!! TTGTGAAATGGAGTTTTTTG+AGG + chr3_4:67568334-67568353 None:intergenic 30.0%
TCAAAAAACTCCATTTCACA+AGG - chr3_4:67568333-67568352 Msa0516130:intron 30.0%
CAAAAAACTCCATTTCACAA+GGG - chr3_4:67568334-67568353 Msa0516130:intron 30.0%
CACTCTTGTGATTCATTATA+AGG - chr3_4:67568727-67568746 Msa0516130:intron 30.0%
AAAGAAAAATCACACATCTG+AGG + chr3_4:67568808-67568827 None:intergenic 30.0%
TGATGAAAGACATTGCATTT+GGG + chr3_4:67568904-67568923 None:intergenic 30.0%
CCGAAGATTATCAAACAATT+AGG - chr3_4:67569017-67569036 Msa0516130:CDS 30.0%
CATCATTGAGATAATGAAGT+TGG + chr3_4:67569057-67569076 None:intergenic 30.0%
! AATCAGATTACACCTTTTAG+TGG - chr3_4:67569377-67569396 Msa0516130:CDS 30.0%
! GTTTTTCACCATTTCCTATT+AGG + chr3_4:67569436-67569455 None:intergenic 30.0%
!!! CAACAACTTGTTTTTACTGT+TGG + chr3_4:67569473-67569492 None:intergenic 30.0%
! AACTTTGAAGAGAAGAACAT+GGG + chr3_4:67569505-67569524 None:intergenic 30.0%
! AAACTTTGAAGAGAAGAACA+TGG + chr3_4:67569506-67569525 None:intergenic 30.0%
AGTGAAGAGATTATAGAACA+GGG + chr3_4:67569589-67569608 None:intergenic 30.0%
TAGTGAAGAGATTATAGAAC+AGG + chr3_4:67569590-67569609 None:intergenic 30.0%
!! AACTCTTTTGGGTAACAATA+AGG - chr3_4:67569703-67569722 Msa0516130:CDS 30.0%
TTTATCAACAGGATAATCTG+CGG + chr3_4:67569912-67569931 None:intergenic 30.0%
!!! ACTGCAAAGATTTGTTTTGC+GGG + chr3_4:67567909-67567928 None:intergenic 35.0%
TGATATCAACTTGAACGACA+CGG - chr3_4:67567974-67567993 Msa0516130:CDS 35.0%
!!! GAGTTAAAGTTTTGAGGGTT+TGG + chr3_4:67568073-67568092 None:intergenic 35.0%
TCTTCTCATACAAGAGAATG+GGG + chr3_4:67568211-67568230 None:intergenic 35.0%
!! GTTTTGAACTTGAATGGGAA+TGG - chr3_4:67568254-67568273 Msa0516130:intron 35.0%
TTTGAACTTGAATGGGAATG+GGG - chr3_4:67568256-67568275 Msa0516130:intron 35.0%
GAAAATGAACCCTTGTGAAA+TGG + chr3_4:67568346-67568365 None:intergenic 35.0%
ATGAGTAAATCCTCAAGAAG+CGG + chr3_4:67568407-67568426 None:intergenic 35.0%
CAATGGGAAACAAGAAGAAA+TGG + chr3_4:67568528-67568547 None:intergenic 35.0%
TCATATGGAGCTTACAATTG+TGG - chr3_4:67568628-67568647 Msa0516130:intron 35.0%
CTTCTTCTTCTTCTTGTGAT+TGG - chr3_4:67568669-67568688 Msa0516130:intron 35.0%
TTTAATTGTTGTCAGTTCGG+AGG - chr3_4:67568835-67568854 Msa0516130:intron 35.0%
ATGAAAGACATTGCATTTGG+GGG + chr3_4:67568902-67568921 None:intergenic 35.0%
GATGAAAGACATTGCATTTG+GGG + chr3_4:67568903-67568922 None:intergenic 35.0%
GTGATGAAAGACATTGCATT+TGG + chr3_4:67568905-67568924 None:intergenic 35.0%
ATGCAATGTCTTTCATCACA+AGG - chr3_4:67568906-67568925 Msa0516130:intron 35.0%
TCACATAAATTATGGAGCCA+AGG - chr3_4:67568946-67568965 Msa0516130:CDS 35.0%
! TTGTTTGATAATCTTCGGGT+GGG + chr3_4:67569015-67569034 None:intergenic 35.0%
! ATTGTTTGATAATCTTCGGG+TGG + chr3_4:67569016-67569035 None:intergenic 35.0%
AGAAAGAAAGGACCACTAAA+AGG + chr3_4:67569392-67569411 None:intergenic 35.0%
CCATTTCCTATTAGGTAGAT+AGG + chr3_4:67569428-67569447 None:intergenic 35.0%
CCTATCTACCTAATAGGAAA+TGG - chr3_4:67569425-67569444 Msa0516130:CDS 35.0%
TATCTCCAAATGTGTATGCA+TGG - chr3_4:67569644-67569663 Msa0516130:CDS 35.0%
CATGTCAAAGAATGTTGTAG+TGG - chr3_4:67569677-67569696 Msa0516130:CDS 35.0%
! TTGTAGTGGTAGAACTCTTT+TGG - chr3_4:67569691-67569710 Msa0516130:CDS 35.0%
! TGTAGTGGTAGAACTCTTTT+GGG - chr3_4:67569692-67569711 Msa0516130:CDS 35.0%
CACAAGAACCAACATTACAA+AGG - chr3_4:67569796-67569815 Msa0516130:CDS 35.0%
TCACTATGCCTTTGTAATGT+TGG + chr3_4:67569807-67569826 None:intergenic 35.0%
! ATTACAAAGGCATAGTGAGT+AGG - chr3_4:67569809-67569828 Msa0516130:CDS 35.0%
ATAGTGAGTAGGATTTCACT+TGG - chr3_4:67569820-67569839 Msa0516130:CDS 35.0%
TAGTGAGTAGGATTTCACTT+GGG - chr3_4:67569821-67569840 Msa0516130:CDS 35.0%
! TCTTGTTATGTCTTTGACGA+CGG - chr3_4:67569935-67569954 Msa0516130:CDS 35.0%
!!! AGCGAGTTTTACTGATTTGT+GGG - chr3_4:67569994-67570013 Msa0516130:CDS 35.0%
! ATTTGTGGGTTCCATTTTGT+CGG - chr3_4:67570008-67570027 Msa0516130:CDS 35.0%
AGTACGTTCAGACTTTGTTA+AGG - chr3_4:67570099-67570118 Msa0516130:CDS 35.0%
GTATGAGTATCAGGAATTCA+AGG - chr3_4:67570138-67570157 Msa0516130:CDS 35.0%
AGTATCAGGAATTCAAGGTT+CGG - chr3_4:67570143-67570162 Msa0516130:CDS 35.0%
!! TTGCTGATTCTGTCTCTTGT+TGG + chr3_4:67567717-67567736 None:intergenic 40.0%
TATTTGTGAGGAGGGCAAAA+AGG + chr3_4:67567772-67567791 None:intergenic 40.0%
! CTTTGAGAGAAGACTTGTGA+GGG + chr3_4:67567796-67567815 None:intergenic 40.0%
AACGAAGAAGAGTTGGCAAA+GGG + chr3_4:67568110-67568129 None:intergenic 40.0%
TAACGAAGAAGAGTTGGCAA+AGG + chr3_4:67568111-67568130 None:intergenic 40.0%
AGCTCTTAACGAAGAAGAGT+TGG + chr3_4:67568117-67568136 None:intergenic 40.0%
ACTCTTCTTCGTTAAGAGCT+TGG - chr3_4:67568117-67568136 Msa0516130:intron 40.0%
CTTCTCATACAAGAGAATGG+GGG + chr3_4:67568210-67568229 None:intergenic 40.0%
!! ATGTGGGCTTTGAGCAAATT+GGG + chr3_4:67568488-67568507 None:intergenic 40.0%
!! AATGTGGGCTTTGAGCAAAT+TGG + chr3_4:67568489-67568508 None:intergenic 40.0%
GGAAGAAGAGTCAGAAATGT+GGG + chr3_4:67568504-67568523 None:intergenic 40.0%
AGGAAGAAGAGTCAGAAATG+TGG + chr3_4:67568505-67568524 None:intergenic 40.0%
TGGGAAACAAGAAGAAATGG+AGG + chr3_4:67568525-67568544 None:intergenic 40.0%
ATTGAAGCTCACACTTTCCT+TGG + chr3_4:67568926-67568945 None:intergenic 40.0%
!! TTATTGGCTTTAGCTGTTGC+TGG - chr3_4:67569111-67569130 Msa0516130:intron 40.0%
ATGTATAGCTACAGCATGAG+TGG + chr3_4:67569327-67569346 None:intergenic 40.0%
GTCCTTTCTTTCTCTGACTT+TGG - chr3_4:67569399-67569418 Msa0516130:CDS 40.0%
CACCAAAGTCAGAGAAAGAA+AGG + chr3_4:67569404-67569423 None:intergenic 40.0%
CTTTCTTTCTCTGACTTTGG+TGG - chr3_4:67569402-67569421 Msa0516130:CDS 40.0%
AGAGATTATAGAACAGGGAG+TGG + chr3_4:67569584-67569603 None:intergenic 40.0%
AGACACCATGCATACACATT+TGG + chr3_4:67569652-67569671 None:intergenic 40.0%
!!! CAGCGAGTTTTACTGATTTG+TGG - chr3_4:67569993-67570012 Msa0516130:CDS 40.0%
AATATGATTCAGGGCTCCTT+AGG + chr3_4:67570048-67570067 None:intergenic 40.0%
GAACGTACTTTGTTCTTGCA+GGG + chr3_4:67570088-67570107 None:intergenic 40.0%
TGAACGTACTTTGTTCTTGC+AGG + chr3_4:67570089-67570108 None:intergenic 40.0%
GGAATTCAAGGTTCGGATTA+AGG - chr3_4:67570150-67570169 Msa0516130:CDS 40.0%
ACTTGTGAGGGCTATTTGTG+AGG + chr3_4:67567784-67567803 None:intergenic 45.0%
!! GCTTTGAGAGAAGACTTGTG+AGG + chr3_4:67567797-67567816 None:intergenic 45.0%
CAAGTCTTCTCTCAAAGCGA+TGG - chr3_4:67567799-67567818 Msa0516130:CDS 45.0%
AGACGTAATCAGCGCTTTAC+CGG - chr3_4:67567851-67567870 Msa0516130:CDS 45.0%
ATGCGACATAGTAGTTCGAC+CGG + chr3_4:67567873-67567892 None:intergenic 45.0%
TTGCAGTATCCTGTCTCTCA+AGG - chr3_4:67567922-67567941 Msa0516130:CDS 45.0%
TATCAACTTGAACGACACGG+AGG - chr3_4:67567977-67567996 Msa0516130:CDS 45.0%
! ACTAGAACATATAAGGGCGC+AGG + chr3_4:67568048-67568067 None:intergenic 45.0%
TCTTCGTTAAGAGCTTGGCT+TGG - chr3_4:67568122-67568141 Msa0516130:intron 45.0%
ATCCTCAAGAAGCGGACATA+CGG + chr3_4:67568399-67568418 None:intergenic 45.0%
! GCTTTGAGCAAATTGGGGAA+GGG + chr3_4:67568482-67568501 None:intergenic 45.0%
!! TGTGGGCTTTGAGCAAATTG+GGG + chr3_4:67568487-67568506 None:intergenic 45.0%
! AAGAGTGTGAAGAGAGAAGC+AGG + chr3_4:67568714-67568733 None:intergenic 45.0%
TAATCTTCGGGTGGGATTTG+AGG + chr3_4:67569007-67569026 None:intergenic 45.0%
TGGTGGCCTATCTACCTAAT+AGG - chr3_4:67569419-67569438 Msa0516130:CDS 45.0%
TGGACAATCGTACCGTAAAG+AGG + chr3_4:67569564-67569583 None:intergenic 45.0%
GGATTTCACTTGGGTGACTT+TGG - chr3_4:67569830-67569849 Msa0516130:CDS 45.0%
TGTTATGTCTTTGACGACGG+CGG - chr3_4:67569938-67569957 Msa0516130:CDS 45.0%
GCTAATAACTTTCGAGGCCA+TGG - chr3_4:67569964-67569983 Msa0516130:CDS 45.0%
ATGGTGTGCTTCCGACAAAA+TGG + chr3_4:67570022-67570041 None:intergenic 45.0%
CAGACTTTGTTAAGGATCGC+AGG - chr3_4:67570107-67570126 Msa0516130:CDS 45.0%
CTTTGTTAAGGATCGCAGGA+AGG - chr3_4:67570111-67570130 Msa0516130:CDS 45.0%
! GAAGGTGCAGTATGAGTATC+AGG - chr3_4:67570129-67570148 Msa0516130:CDS 45.0%
!! AAATAATAAAAAAATAAAAG+AGG + chr3_4:67569182-67569201 None:intergenic 5.0%
GTGAGGGCTATTTGTGAGGA+GGG + chr3_4:67567780-67567799 None:intergenic 50.0%
TGTGAGGGCTATTTGTGAGG+AGG + chr3_4:67567781-67567800 None:intergenic 50.0%
! CAAAGCGATGGAATCGACTC+TGG - chr3_4:67567811-67567830 Msa0516130:CDS 50.0%
!! GCGCTGATTACGTCTGCTTT+AGG + chr3_4:67567846-67567865 None:intergenic 50.0%
ACGACACGGAGGATGATCAT+CGG - chr3_4:67567988-67568007 Msa0516130:intron 50.0%
TACAAGAGAATGGGGGCAGT+GGG + chr3_4:67568203-67568222 None:intergenic 50.0%
ATACAAGAGAATGGGGGCAG+TGG + chr3_4:67568204-67568223 None:intergenic 50.0%
!! GGCTTTGAGCAAATTGGGGA+AGG + chr3_4:67568483-67568502 None:intergenic 50.0%
AGTGTGAAGAGAGAAGCAGG+CGG + chr3_4:67568711-67568730 None:intergenic 50.0%
TGCATTTGGGGGACAAACTG+CGG + chr3_4:67568891-67568910 None:intergenic 50.0%
! GCAGGATGATGGCCTCTTTA+CGG - chr3_4:67569549-67569568 Msa0516130:CDS 50.0%
ACTTTGGCGTCTGGTGACTT+TGG - chr3_4:67569846-67569865 Msa0516130:CDS 50.0%
GTTATGTCTTTGACGACGGC+GGG - chr3_4:67569939-67569958 Msa0516130:CDS 50.0%
CGGGTCGCTAATAACTTTCG+AGG - chr3_4:67569958-67569977 Msa0516130:CDS 50.0%
! GGAAGCACACCATTGACCTA+AGG - chr3_4:67570029-67570048 Msa0516130:CDS 50.0%
TTCAGGGCTCCTTAGGTCAA+TGG + chr3_4:67570041-67570060 None:intergenic 50.0%
GTGAGGAGGGCAAAAAGGAG+AGG + chr3_4:67567767-67567786 None:intergenic 55.0%
TATCCTGTCTCTCAAGGCGC+TGG - chr3_4:67567928-67567947 Msa0516130:CDS 55.0%
CGACACGGAGGATGATCATC+GGG - chr3_4:67567989-67568008 Msa0516130:intron 55.0%
! TATAAGGGCGCAGGCGATGT+CGG + chr3_4:67568039-67568058 None:intergenic 55.0%
CTCCGTATGTCCGCTTCTTG+AGG - chr3_4:67568394-67568413 Msa0516130:intron 55.0%
TTGGGTTTCCCTCGCATTGC+AGG - chr3_4:67569531-67569550 Msa0516130:CDS 55.0%
TCCCTCGCATTGCAGGATGA+TGG - chr3_4:67569538-67569557 Msa0516130:CDS 55.0%
GCCATCATCCTGCAATGCGA+GGG + chr3_4:67569542-67569561 None:intergenic 55.0%
ACTTGGGTGACTTTGGCGTC+TGG - chr3_4:67569837-67569856 Msa0516130:CDS 55.0%
CAACAGGATAATCTGCGGCG+AGG + chr3_4:67569907-67569926 None:intergenic 55.0%
AACTTTCGAGGCCATGGCAG+AGG - chr3_4:67569970-67569989 Msa0516130:CDS 55.0%
AAAACTCGCTGCCTCTGCCA+TGG + chr3_4:67569984-67570003 None:intergenic 55.0%
TCTCCAGCGCCTTGAGAGAC+AGG + chr3_4:67567934-67567953 None:intergenic 60.0%
TGTCAGTTCGGAGGCTGCTG+TGG - chr3_4:67568844-67568863 Msa0516130:intron 60.0%
AGGCTGCTGTGGCGAAGAAG+TGG - chr3_4:67568855-67568874 Msa0516130:intron 60.0%
GGCCATCATCCTGCAATGCG+AGG + chr3_4:67569543-67569562 None:intergenic 60.0%
TGGCGTCTGGTGACTTTGGC+TGG - chr3_4:67569850-67569869 Msa0516130:CDS 60.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 67567708 67570204 67567708 ID=Msa0516130;Name=Msa0516130
chr3_4 mRNA 67567708 67570204 67567708 ID=Msa0516130-mRNA-1;Parent=Msa0516130;Name=Msa0516130-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|0.5|0|0.66|1|1|3|0|484
chr3_4 exon 67569164 67570204 67569164 ID=Msa0516130-mRNA-1:exon:17631;Parent=Msa0516130-mRNA-1
chr3_4 exon 67568939 67569062 67568939 ID=Msa0516130-mRNA-1:exon:17630;Parent=Msa0516130-mRNA-1
chr3_4 exon 67567708 67567997 67567708 ID=Msa0516130-mRNA-1:exon:17629;Parent=Msa0516130-mRNA-1
chr3_4 CDS 67569164 67570204 67569164 ID=Msa0516130-mRNA-1:cds;Parent=Msa0516130-mRNA-1
chr3_4 CDS 67568939 67569062 67568939 ID=Msa0516130-mRNA-1:cds;Parent=Msa0516130-mRNA-1
chr3_4 CDS 67567708 67567997 67567708 ID=Msa0516130-mRNA-1:cds;Parent=Msa0516130-mRNA-1
Gene Sequence

>Msa0516130

ATGGCACCAACAAGAGACAGAATCAGCAATTTACCAGATGAATTAATTCATCACATCCTCTCCTTTTTGCCCTCCTCACAAATAGCCCTCACAAGTCTTCTCTCAAAGCGATGGAATCGACTCTGGTTAACAATGCCTAAAGCAGACGTAATCAGCGCTTTACCGGTCGAACTACTATGTCGCATTCTGTCTCTTTTACCCGCAAAACAAATCTTTGCAGTATCCTGTCTCTCAAGGCGCTGGAGACGACTCCTTGAAAGAATTCGTGATATCAACTTGAACGACACGGAGGATGATCATCGGGACAAAGACGCCTATTTTGATTATACCGACATCGCCTGCGCCCTTATATGTTCTAGTGACCAAACCCTCAAAACTTTAACTCTTTTGTCTGCGTCTCCCTTTGCCAACTCTTCTTCGTTAAGAGCTTGGCTTGGCGAAGCAATCTATAGAAAAGTTGAACATGTCAATCTCACTTTTTTGCAGCATTCTCCCACTGCCCCCATTCTCTTGTATGAGAAGATTCTAATGTGTTCAACTCTTGTTGTTTTGAACTTGAATGGGAATGGGGTCTTAACTATTGACTATGTTGATAACTATAGTACTAGTACTAATTTGCCAAACCTCAAAAAACTCCATTTCACAAGGGTTCATTTTCTAAAGCTTCAATTTCTTATTAAGATTCTCTCCGTATGTCCGCTTCTTGAGGATTTACTCATCAAAAATGTTACAGTTGATGATCATAATGCTGCTACAGCAGATATGTTAAAACCCTTCCCCAATTTGCTCAAAGCCCACATTTCTGACTCTTCTTCCTCCATTTCTTCTTGTTTCCCATTGAACTTGTTTTACAATGTGAACTTTCTTCGCACACAAGTCACACTACAACATCAAGACACTACTCAATTTCTCAATTTAACTCATATGGAGCTTACAATTGTGGACGACGAATATTCTTCTTCTTCTTCTTCTTCTTGTGATTGGTTAAACAAATTCGTCTCCGCCTGCTTCTCTCTTCACACTCTTGTGATTCATTATAAGTTCGGAGGCTGCTGTGGCGAAGAAGTGGTGATATGCATCCGCAGTTTGTCCCCCAAATGCAATGTCTTTCATCACAAGGAAAGTGTGAGCTTCAATTCACATAAATTATGGAGCCAAGGAAAGTATTATCCTGTTGATAAACTATCTTGTTATGTCTTTGACGACGGCGGGTCGCTAATAACTTTCGAGGCCATGGCAGAGGCAGCGAGTTTTACTGATTTGTGGGTTCCATTTTGTCGGAAGCACACCATTGACCTAAGGAGCCCTGAATCATATTTTAGTATGCAGAGAGATCCCTGCAAGAACAAAGTACGTTCAGACTTTGTTAAGGATCGCAGGAAGGTGCAGTATGAGTATCAGGAATTCAAGGTTCGGATTAAGGTGCAGTATGATCATTATAATAATGGAAATTAA
Protein sequence

>Msa0516130

MAPTRDRISNLPDELIHHILSFLPSSQIALTSLLSKRWNRLWLTMPKADVISALPVELLCRILSLLPAKQIFAVSCLSRRWRRLLERIRDINLNDTEDDHRDKDAYFDYTDIACALICSSDQTLKTLTLLSASPFANSSSLRAWLGEAIYRKVEHVNLTFLQHSPTAPILLYEKILMCSTLVVLNLNGNGVLTIDYVDNYSTSTNLPNLKKLHFTRVHFLKLQFLIKILSVCPLLEDLLIKNVTVDDHNAATADMLKPFPNLLKAHISDSSSSISSCFPLNLFYNVNFLRTQVTLQHQDTTQFLNLTHMELTIVDDEYSSSSSSSCDWLNKFVSACFSLHTLVIHYKFGGCCGEEVVICIRSLSPKCNVFHHKESVSFNSHKLWSQGKYYPVDKLSCYVFDDGGSLITFEAMAEAASFTDLWVPFCRKHTIDLRSPESYFSMQRDPCKNKVRSDFVKDRRKVQYEYQEFKVRIKVQYDHYNNGN*