AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0525920

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0525920 MtrunA17_Chr1g0166291 60.506 514 174 12 5 505 758 1255 1.17e-164 500
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 90 sgRNAs with CRISPR-Local

Find 151 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCCGAAAGCCTTAAGATTT+TGG 0.090861 3_4:-79563331 None:intergenic
TTCTTTCTGATTTATATTTC+TGG 0.160236 3_4:+79564194 Msa0525920:CDS
AAGAGAACTGCTTCAATTTC+AGG 0.163473 3_4:-79562820 None:intergenic
CCGACAGCTGGAACGGTATT+TGG 0.177666 3_4:+79563419 Msa0525920:CDS
AACAATTCAATATTTGTAAA+TGG 0.218628 3_4:+79563657 Msa0525920:CDS
CAAGTGATGTTTGGATTATC+AGG 0.272548 3_4:-79563729 None:intergenic
ACCCAAAATCTTAAGGCTTT+CGG 0.289769 3_4:+79563329 Msa0525920:CDS
GCTCGTGGCCGTGAAACTTT+TGG 0.321564 3_4:-79563099 None:intergenic
TGAAGCAGTTCTCTTATTGC+AGG 0.330706 3_4:+79562828 Msa0525920:CDS
TGGAGAGAAAATAGTTACTA+TGG 0.340326 3_4:+79563917 Msa0525920:CDS
TTGTTGTTGTTGCCAATACT+TGG 0.362848 3_4:-79562137 None:intergenic
CTATCCAGCCATTCACTTCA+AGG 0.367802 3_4:+79561927 Msa0525920:CDS
ATTTCAAATTAGTTCTTCAT+AGG 0.372382 3_4:+79561278 Msa0525920:CDS
CATTTCAACCCAAAATCTTA+AGG 0.374331 3_4:+79563322 Msa0525920:CDS
ATATCTAAAGCTTCATTCAT+TGG 0.375973 3_4:+79563957 Msa0525920:CDS
CGATTCTTCTCGTCTTGATT+CGG 0.381521 3_4:-79563793 None:intergenic
AAGAAGCAATTGAGAAAGAA+AGG 0.383115 3_4:+79563559 Msa0525920:CDS
TGAAAATGAGAATCAGCATT+TGG 0.398342 3_4:-79561978 None:intergenic
TGCATCTGACAAGTGATGTT+TGG 0.400511 3_4:-79563738 None:intergenic
GATATCACAGTAGGGTCTAA+TGG 0.409358 3_4:+79562736 Msa0525920:CDS
CAAATACCGTTCCAGCTGTC+GGG 0.412049 3_4:-79563418 None:intergenic
GAAAATAAATTGAGCGAAAG+TGG 0.424067 3_4:+79562625 Msa0525920:CDS
TACTGTGATATCATATGTAT+TGG 0.436564 3_4:-79562725 None:intergenic
CATGATGAGTATGGTCTCAT+TGG 0.441317 3_4:+79563897 Msa0525920:CDS
TAAGCAAGATCAATGCATAA+AGG 0.450069 3_4:+79562675 Msa0525920:CDS
AGAAGCAACTTCACTGATAG+TGG 0.454090 3_4:+79563602 Msa0525920:CDS
CTTCTCGTCTTGATTCGGTT+CGG 0.459344 3_4:-79563788 None:intergenic
AAATGTGGTGTCCGGTCTCT+TGG 0.461743 3_4:-79563304 None:intergenic
CATAGGACAACAACCAAATC+AGG 0.467136 3_4:+79561295 Msa0525920:CDS
TCTGCTCTACATTTCCCTGC+TGG 0.469329 3_4:-79563076 None:intergenic
GCCACGAGCATGCTTAAATC+AGG 0.472448 3_4:+79563113 Msa0525920:CDS
CGAATCAAGACGAGAAGAAT+CGG 0.472470 3_4:+79563794 Msa0525920:CDS
TGTTGTTGTTGCCAATACTT+GGG 0.478593 3_4:-79562136 None:intergenic
TGATCTTGCTTAGAATCTTG+TGG 0.479810 3_4:-79562664 None:intergenic
TGTGCCACAATTGACAAGCT+TGG 0.484968 3_4:+79563167 Msa0525920:CDS
TTGGGTTGAAATGTGGTGTC+CGG 0.493063 3_4:-79563312 None:intergenic
AGAAGCAATTGAGAAAGAAA+GGG 0.495359 3_4:+79563560 Msa0525920:CDS
TCAGTGCCTCATGATGAGTA+TGG 0.502689 3_4:+79563888 Msa0525920:CDS
GTGTCATCTATTTCACCTAA+AGG 0.502811 3_4:+79564068 Msa0525920:CDS
GTCTGTGCCATGACTAGAAC+AGG 0.509770 3_4:-79564005 None:intergenic
TTTCTGAACCACCGAGAGAT+CGG 0.510422 3_4:-79563367 None:intergenic
TCTCGAGACTCCCCGACAGC+TGG 0.512920 3_4:+79563407 Msa0525920:CDS
ATCATATGTATTGGCCATGT+CGG 0.520487 3_4:-79562716 None:intergenic
TTCTGAACCACCGAGAGATC+GGG 0.520899 3_4:-79563366 None:intergenic
GCATATCTATAAATACCAGC+AGG 0.522867 3_4:+79563061 Msa0525920:CDS
GACATGGTGTCCCGATCTCT+CGG 0.524228 3_4:+79563356 Msa0525920:CDS
AATTCAATATTTGTAAATGG+TGG 0.526172 3_4:+79563660 Msa0525920:CDS
CTCGTCTTGATTCGGTTCGG+AGG 0.535593 3_4:-79563785 None:intergenic
GGAGTCTCGAGAGCAACAAC+GGG 0.536250 3_4:-79563396 None:intergenic
ATTGCCTTGAAGTGAATGGC+TGG 0.536724 3_4:-79561931 None:intergenic
ATCCAGGATCACCAAGAGAC+CGG 0.541341 3_4:+79563293 Msa0525920:intron
CAACAATGGAGAAAATTTCA+AGG 0.542664 3_4:+79562154 Msa0525920:CDS
CGGAGGTTGAGATTGAAGCT+TGG 0.544850 3_4:-79563768 None:intergenic
AATACATATGATATCACAGT+AGG 0.544931 3_4:+79562727 Msa0525920:CDS
ATTCATTGCCTTGAAGTGAA+TGG 0.546113 3_4:-79561935 None:intergenic
AGAGCAGACCAAAAGTTTCA+CGG 0.547609 3_4:+79563091 Msa0525920:CDS
CAAAAGTCAGATGGAAGAGA+TGG 0.549084 3_4:+79562062 Msa0525920:CDS
CCAAATACCGTTCCAGCTGT+CGG 0.551035 3_4:-79563419 None:intergenic
CAGGAACCACAGAAGTATCA+AGG 0.556349 3_4:-79562801 None:intergenic
TAAATGGTGGTTATGAAACA+AGG 0.558662 3_4:+79563673 Msa0525920:CDS
ATTGTTTGAACTTAATTCAG+TGG 0.559516 3_4:-79563640 None:intergenic
ATGGCCAAGCTTGTCAATTG+TGG 0.567580 3_4:-79563171 None:intergenic
TCTTGATTGCACACATGACA+AGG 0.567651 3_4:+79562866 Msa0525920:CDS
GGGAGTCTCGAGAGCAACAA+CGG 0.580719 3_4:-79563397 None:intergenic
TTATGTCCGCAAAAGTCAGA+TGG 0.580735 3_4:+79562053 Msa0525920:CDS
TAAGGCTTTCGGAGGAGACA+TGG 0.581347 3_4:+79563340 Msa0525920:CDS
TCTGAAGACTTATCAAGCAA+TGG 0.583115 3_4:-79563825 None:intergenic
AGAAGCTCCTGTTCTAGTCA+TGG 0.584849 3_4:+79563998 Msa0525920:CDS
CAAATCAGGCAAAAGATCAA+AGG 0.587061 3_4:+79561309 Msa0525920:CDS
AGATGATAGCTTGTCAAACA+AGG 0.589755 3_4:+79563845 Msa0525920:CDS
AGACTCCCCGACAGCTGGAA+CGG 0.592227 3_4:+79563412 Msa0525920:CDS
TAATGTCCTTGATACTTCTG+TGG 0.593110 3_4:+79562795 Msa0525920:CDS
GTTGTTGTTGCCAATACTTG+GGG 0.597201 3_4:-79562135 None:intergenic
GGGTCTAATGGAGACTGCAA+TGG 0.599019 3_4:+79562748 Msa0525920:CDS
AAATACCGTTCCAGCTGTCG+GGG 0.609455 3_4:-79563417 None:intergenic
CAGACACAAGATAACAATGC+TGG 0.612904 3_4:+79564023 Msa0525920:CDS
CAAAATCTTAAGGCTTTCGG+AGG 0.622089 3_4:+79563332 Msa0525920:CDS
ACCTGATTTAAGCATGCTCG+TGG 0.633778 3_4:-79563114 None:intergenic
AGTATTGGCAACAACAACAA+TGG 0.643813 3_4:+79562140 Msa0525920:CDS
AAACAAGGGACAGTGTAACA+AGG 0.644641 3_4:+79563688 Msa0525920:CDS
CATATCTATAAATACCAGCA+GGG 0.645811 3_4:+79563062 Msa0525920:CDS
ATACATATGATATCACAGTA+GGG 0.650083 3_4:+79562728 Msa0525920:CDS
ATGAGACCATACTCATCATG+AGG 0.664138 3_4:-79563894 None:intergenic
TCAAGAGTACAAGTCCGACA+TGG 0.668203 3_4:+79562702 Msa0525920:CDS
CTTGATTGCACACATGACAA+GGG 0.676782 3_4:+79562867 Msa0525920:CDS
GGAAAGAAAAGCGCAAATTG+CGG 0.677151 3_4:+79564089 Msa0525920:CDS
AAATGGTGGTTATGAAACAA+GGG 0.678393 3_4:+79563674 Msa0525920:CDS
TTGTTGTTGCCAATACTTGG+GGG 0.678840 3_4:-79562134 None:intergenic
CGAGCATGCTTAAATCAGGT+AGG 0.695668 3_4:+79563117 Msa0525920:CDS
ATGGTGTCCCGATCTCTCGG+TGG 0.717382 3_4:+79563359 Msa0525920:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAATTTTTTGTTTAAACT+AGG + chr3_4:79562336-79562355 Msa0525920:intron 10.0%
!!! ATTAATTTGTTTATCAAAAT+AGG + chr3_4:79562591-79562610 Msa0525920:intron 10.0%
!!! TTAATTTGTTTATCAAAATA+GGG + chr3_4:79562592-79562611 Msa0525920:intron 10.0%
!! AAATTTCTTTATCATTACAT+AGG - chr3_4:79562946-79562965 None:intergenic 15.0%
!!! TTTCATTTTTACTTTGTATT+TGG + chr3_4:79563214-79563233 Msa0525920:intron 15.0%
!!! TTCATTTTTACTTTGTATTT+GGG + chr3_4:79563215-79563234 Msa0525920:intron 15.0%
!! AACAATTCAATATTTGTAAA+TGG + chr3_4:79563657-79563676 Msa0525920:CDS 15.0%
!!! TAATTCTAAAGGTTTTAATT+TGG - chr3_4:79564154-79564173 None:intergenic 15.0%
!!! ATTTCAAATTAGTTCTTCAT+AGG + chr3_4:79561278-79561297 Msa0525920:CDS 20.0%
!! ATGTTTCAACAATTAAATCT+AGG - chr3_4:79561709-79561728 None:intergenic 20.0%
!! AACATCATTAGATTTCAATT+TGG + chr3_4:79561724-79561743 Msa0525920:intron 20.0%
!!! TTTAATATAAGTTTTCCATG+AGG - chr3_4:79562471-79562490 None:intergenic 20.0%
!! ATAAGAGGTTATGTATTATA+AGG - chr3_4:79562538-79562557 None:intergenic 20.0%
!! TGTAATGATAAAGAAATTTG+AGG + chr3_4:79562947-79562966 Msa0525920:intron 20.0%
!! TATTCTTCCTGATTTATTAT+AGG - chr3_4:79563011-79563030 None:intergenic 20.0%
!! TGTTAACAATTCAATCTATA+TGG + chr3_4:79563524-79563543 Msa0525920:intron 20.0%
!! AATTCAATATTTGTAAATGG+TGG + chr3_4:79563660-79563679 Msa0525920:CDS 20.0%
!! ACAAAAATGTGTAATTCTAA+AGG - chr3_4:79564165-79564184 None:intergenic 20.0%
!! TTCTTTCTGATTTATATTTC+TGG + chr3_4:79564194-79564213 Msa0525920:CDS 20.0%
! TTCTATAAACTCTCAAGAAT+AGG + chr3_4:79561558-79561577 Msa0525920:intron 25.0%
!! ACTTAATCTTTTCATTGTCT+TGG + chr3_4:79561785-79561804 Msa0525920:intron 25.0%
!! TGGTTTTCTAAACTTAAAGA+TGG - chr3_4:79562427-79562446 None:intergenic 25.0%
! TACTGTGATATCATATGTAT+TGG - chr3_4:79562728-79562747 None:intergenic 25.0%
! AATACATATGATATCACAGT+AGG + chr3_4:79562727-79562746 Msa0525920:CDS 25.0%
! ATACATATGATATCACAGTA+GGG + chr3_4:79562728-79562747 Msa0525920:CDS 25.0%
! ATGTTGTTAGAAAGTAAACA+TGG - chr3_4:79563193-79563212 None:intergenic 25.0%
!! ATTTTTACTTTGTATTTGGG+TGG + chr3_4:79563218-79563237 Msa0525920:intron 25.0%
! ATTGTTTGAACTTAATTCAG+TGG - chr3_4:79563643-79563662 None:intergenic 25.0%
!! ATATCTAAAGCTTCATTCAT+TGG + chr3_4:79563957-79563976 Msa0525920:CDS 25.0%
AAGCATTTGTGAAACTGTTT+AGG + chr3_4:79561485-79561504 Msa0525920:intron 30.0%
! CAATTAAATCTAGGCTTGTT+TGG - chr3_4:79561700-79561719 None:intergenic 30.0%
ATCAAATGATTCAGAACTTC+AGG + chr3_4:79561866-79561885 Msa0525920:intron 30.0%
TGAAAATGAGAATCAGCATT+TGG - chr3_4:79561981-79562000 None:intergenic 30.0%
! TTTTCAACAGTTGAAACATG+AGG + chr3_4:79561995-79562014 Msa0525920:CDS 30.0%
TGAGGTTTCAGAAAAAGATA+TGG + chr3_4:79562013-79562032 Msa0525920:CDS 30.0%
CAACAATGGAGAAAATTTCA+AGG + chr3_4:79562154-79562173 Msa0525920:CDS 30.0%
ACAAATAAGTCAATCCAAAC+AGG - chr3_4:79562189-79562208 None:intergenic 30.0%
AGACTATTTGAGAGAGTTTA+TGG + chr3_4:79562236-79562255 Msa0525920:intron 30.0%
! TTTTCCACCTATATCTCTTT+AGG + chr3_4:79562287-79562306 Msa0525920:intron 30.0%
ATAAGCACTTATCACATAAG+AGG - chr3_4:79562553-79562572 None:intergenic 30.0%
GAAAATAAATTGAGCGAAAG+TGG + chr3_4:79562625-79562644 Msa0525920:CDS 30.0%
TAAGCAAGATCAATGCATAA+AGG + chr3_4:79562675-79562694 Msa0525920:CDS 30.0%
ATCATTACATAGGAAGATTC+TGG - chr3_4:79562936-79562955 None:intergenic 30.0%
TATGGTGCCTATAATAAATC+AGG + chr3_4:79563001-79563020 Msa0525920:intron 30.0%
CATATCTATAAATACCAGCA+GGG + chr3_4:79563062-79563081 Msa0525920:CDS 30.0%
!!! TAAGATTTTGGGTTGAAATG+TGG - chr3_4:79563322-79563341 None:intergenic 30.0%
CATTTCAACCCAAAATCTTA+AGG + chr3_4:79563322-79563341 Msa0525920:CDS 30.0%
AAGAAGCAATTGAGAAAGAA+AGG + chr3_4:79563559-79563578 Msa0525920:CDS 30.0%
AGAAGCAATTGAGAAAGAAA+GGG + chr3_4:79563560-79563579 Msa0525920:CDS 30.0%
TAAATGGTGGTTATGAAACA+AGG + chr3_4:79563673-79563692 Msa0525920:CDS 30.0%
AAATGGTGGTTATGAAACAA+GGG + chr3_4:79563674-79563693 Msa0525920:CDS 30.0%
TGGAGAGAAAATAGTTACTA+TGG + chr3_4:79563917-79563936 Msa0525920:CDS 30.0%
!! TAACAATGCTGGTTCAAAAA+TGG + chr3_4:79564034-79564053 Msa0525920:CDS 30.0%
! CTTTGATCTTTTGCCTGATT+TGG - chr3_4:79561311-79561330 None:intergenic 35.0%
CAAATCAGGCAAAAGATCAA+AGG + chr3_4:79561309-79561328 Msa0525920:CDS 35.0%
! AAACTGTTTAGGATAGCTTG+TGG + chr3_4:79561496-79561515 Msa0525920:intron 35.0%
ATTCATTGCCTTGAAGTGAA+TGG - chr3_4:79561938-79561957 None:intergenic 35.0%
! TGTTGTTGTTGCCAATACTT+GGG - chr3_4:79562139-79562158 None:intergenic 35.0%
! TTGTTGTTGTTGCCAATACT+TGG - chr3_4:79562140-79562159 None:intergenic 35.0%
!! AGTATTGGCAACAACAACAA+TGG + chr3_4:79562140-79562159 Msa0525920:CDS 35.0%
CTATCCTAAAGAGATATAGG+TGG - chr3_4:79562294-79562313 None:intergenic 35.0%
CGACTATCCTAAAGAGATAT+AGG - chr3_4:79562297-79562316 None:intergenic 35.0%
TGATCTTGCTTAGAATCTTG+TGG - chr3_4:79562667-79562686 None:intergenic 35.0%
! ATCATATGTATTGGCCATGT+CGG - chr3_4:79562719-79562738 None:intergenic 35.0%
TAATGTCCTTGATACTTCTG+TGG + chr3_4:79562795-79562814 Msa0525920:CDS 35.0%
AAGAGAACTGCTTCAATTTC+AGG - chr3_4:79562823-79562842 None:intergenic 35.0%
TTATAGGCACCATATCAGAA+TGG - chr3_4:79562995-79563014 None:intergenic 35.0%
GCATATCTATAAATACCAGC+AGG + chr3_4:79563061-79563080 Msa0525920:CDS 35.0%
AAGAATGAGCTAAATGATCC+AGG + chr3_4:79563277-79563296 Msa0525920:intron 35.0%
ACCCAAAATCTTAAGGCTTT+CGG + chr3_4:79563329-79563348 Msa0525920:CDS 35.0%
! TCCGAAAGCCTTAAGATTTT+GGG - chr3_4:79563333-79563352 None:intergenic 35.0%
CAAGTGATGTTTGGATTATC+AGG - chr3_4:79563732-79563751 None:intergenic 35.0%
TCTGAAGACTTATCAAGCAA+TGG - chr3_4:79563828-79563847 None:intergenic 35.0%
! AGATGATAGCTTGTCAAACA+AGG + chr3_4:79563845-79563864 Msa0525920:CDS 35.0%
GTGTCATCTATTTCACCTAA+AGG + chr3_4:79564068-79564087 Msa0525920:CDS 35.0%
! TTTGCGCTTTTCTTTCCTTT+AGG - chr3_4:79564086-79564105 None:intergenic 35.0%
CATAGGACAACAACCAAATC+AGG + chr3_4:79561295-79561314 Msa0525920:CDS 40.0%
TTATGTCCGCAAAAGTCAGA+TGG + chr3_4:79562053-79562072 Msa0525920:CDS 40.0%
! TCTCTTCCATCTGACTTTTG+CGG - chr3_4:79562062-79562081 None:intergenic 40.0%
CAAAAGTCAGATGGAAGAGA+TGG + chr3_4:79562062-79562081 Msa0525920:CDS 40.0%
ACATTTGATCCCCCAAGTAT+TGG + chr3_4:79562125-79562144 Msa0525920:CDS 40.0%
! TTGTTGTTGCCAATACTTGG+GGG - chr3_4:79562137-79562156 None:intergenic 40.0%
! GTTGTTGTTGCCAATACTTG+GGG - chr3_4:79562138-79562157 None:intergenic 40.0%
TCTCGGATACGGTTGTAAAA+TGG - chr3_4:79562393-79562412 None:intergenic 40.0%
! ATTTTACAACCGTATCCGAG+AGG + chr3_4:79562392-79562411 Msa0525920:intron 40.0%
AGTTCAAATCCTCTCGGATA+CGG - chr3_4:79562404-79562423 None:intergenic 40.0%
AGATGGAGTTCAAATCCTCT+CGG - chr3_4:79562410-79562429 None:intergenic 40.0%
GATATCACAGTAGGGTCTAA+TGG + chr3_4:79562736-79562755 Msa0525920:CDS 40.0%
TGAAGCAGTTCTCTTATTGC+AGG + chr3_4:79562828-79562847 Msa0525920:CDS 40.0%
TCTTGATTGCACACATGACA+AGG + chr3_4:79562866-79562885 Msa0525920:CDS 40.0%
CTTGATTGCACACATGACAA+GGG + chr3_4:79562867-79562886 Msa0525920:CDS 40.0%
AGAGCAATGCCATTCTGATA+TGG + chr3_4:79562983-79563002 Msa0525920:intron 40.0%
AGAGCAGACCAAAAGTTTCA+CGG + chr3_4:79563091-79563110 Msa0525920:CDS 40.0%
TTACTTTGTATTTGGGTGGC+CGG + chr3_4:79563222-79563241 Msa0525920:intron 40.0%
CTATGATGATTCCCTAACAC+CGG - chr3_4:79563244-79563263 None:intergenic 40.0%
! ATTTAGCTCATTCTTGCTCC+CGG - chr3_4:79563272-79563291 None:intergenic 40.0%
! CTCCGAAAGCCTTAAGATTT+TGG - chr3_4:79563334-79563353 None:intergenic 40.0%
CAAAATCTTAAGGCTTTCGG+AGG + chr3_4:79563332-79563351 Msa0525920:CDS 40.0%
AGAAGCAACTTCACTGATAG+TGG + chr3_4:79563602-79563621 Msa0525920:CDS 40.0%
AAACAAGGGACAGTGTAACA+AGG + chr3_4:79563688-79563707 Msa0525920:CDS 40.0%
TGCATCTGACAAGTGATGTT+TGG - chr3_4:79563741-79563760 None:intergenic 40.0%
CGATTCTTCTCGTCTTGATT+CGG - chr3_4:79563796-79563815 None:intergenic 40.0%
CGAATCAAGACGAGAAGAAT+CGG + chr3_4:79563794-79563813 Msa0525920:CDS 40.0%
ATGAGACCATACTCATCATG+AGG - chr3_4:79563897-79563916 None:intergenic 40.0%
CATGATGAGTATGGTCTCAT+TGG + chr3_4:79563897-79563916 Msa0525920:CDS 40.0%
CAGACACAAGATAACAATGC+TGG + chr3_4:79564023-79564042 Msa0525920:CDS 40.0%
! GGAAAGAAAAGCGCAAATTG+CGG + chr3_4:79564089-79564108 Msa0525920:CDS 40.0%
CTATCCAGCCATTCACTTCA+AGG + chr3_4:79561927-79561946 Msa0525920:CDS 45.0%
ATTGCCTTGAAGTGAATGGC+TGG - chr3_4:79561934-79561953 None:intergenic 45.0%
CAAGGTATGTAGAGCCTGTT+TGG + chr3_4:79562172-79562191 Msa0525920:intron 45.0%
GTCAGCTTAAGGCAAGAGTT+TGG - chr3_4:79562447-79562466 None:intergenic 45.0%
! TTCCATGAGGTGTCAGCTTA+AGG - chr3_4:79562458-79562477 None:intergenic 45.0%
! TCAAGAGTACAAGTCCGACA+TGG + chr3_4:79562702-79562721 Msa0525920:CDS 45.0%
!! CAGGAACCACAGAAGTATCA+AGG - chr3_4:79562804-79562823 None:intergenic 45.0%
ACCTGATTTAAGCATGCTCG+TGG - chr3_4:79563117-79563136 None:intergenic 45.0%
CGAGCATGCTTAAATCAGGT+AGG + chr3_4:79563117-79563136 Msa0525920:CDS 45.0%
TGTGCCACAATTGACAAGCT+TGG + chr3_4:79563167-79563186 Msa0525920:CDS 45.0%
ATGGCCAAGCTTGTCAATTG+TGG - chr3_4:79563174-79563193 None:intergenic 45.0%
! CGGTGTTAGGGAATCATCAT+AGG + chr3_4:79563242-79563261 Msa0525920:intron 45.0%
!! TTGGGTTGAAATGTGGTGTC+CGG - chr3_4:79563315-79563334 None:intergenic 45.0%
TTTCTGAACCACCGAGAGAT+CGG - chr3_4:79563370-79563389 None:intergenic 45.0%
!! CTTCTCGTCTTGATTCGGTT+CGG - chr3_4:79563791-79563810 None:intergenic 45.0%
TCAGTGCCTCATGATGAGTA+TGG + chr3_4:79563888-79563907 Msa0525920:CDS 45.0%
AGAAGCTCCTGTTCTAGTCA+TGG + chr3_4:79563998-79564017 Msa0525920:CDS 45.0%
TGCCTTAAGCTGACACCTCA+TGG + chr3_4:79562453-79562472 Msa0525920:intron 50.0%
GGGTCTAATGGAGACTGCAA+TGG + chr3_4:79562748-79562767 Msa0525920:CDS 50.0%
TCTGCTCTACATTTCCCTGC+TGG - chr3_4:79563079-79563098 None:intergenic 50.0%
GCCACGAGCATGCTTAAATC+AGG + chr3_4:79563113-79563132 Msa0525920:CDS 50.0%
! TATTTGGGTGGCCGGTGTTA+GGG + chr3_4:79563230-79563249 Msa0525920:intron 50.0%
GGGAATCATCATAGGAGAGC+CGG + chr3_4:79563250-79563269 Msa0525920:intron 50.0%
GGAATCATCATAGGAGAGCC+GGG + chr3_4:79563251-79563270 Msa0525920:intron 50.0%
ATCCAGGATCACCAAGAGAC+CGG + chr3_4:79563293-79563312 Msa0525920:intron 50.0%
AAATGTGGTGTCCGGTCTCT+TGG - chr3_4:79563307-79563326 None:intergenic 50.0%
TAAGGCTTTCGGAGGAGACA+TGG + chr3_4:79563340-79563359 Msa0525920:CDS 50.0%
TTCTGAACCACCGAGAGATC+GGG - chr3_4:79563369-79563388 None:intergenic 50.0%
AAATACCGTTCCAGCTGTCG+GGG - chr3_4:79563420-79563439 None:intergenic 50.0%
CAAATACCGTTCCAGCTGTC+GGG - chr3_4:79563421-79563440 None:intergenic 50.0%
CCAAATACCGTTCCAGCTGT+CGG - chr3_4:79563422-79563441 None:intergenic 50.0%
CGGAGGTTGAGATTGAAGCT+TGG - chr3_4:79563771-79563790 None:intergenic 50.0%
GTCTGTGCCATGACTAGAAC+AGG - chr3_4:79564008-79564027 None:intergenic 50.0%
! GCTCGTGGCCGTGAAACTTT+TGG - chr3_4:79563102-79563121 None:intergenic 55.0%
GTATTTGGGTGGCCGGTGTT+AGG + chr3_4:79563229-79563248 Msa0525920:intron 55.0%
GACATGGTGTCCCGATCTCT+CGG + chr3_4:79563356-79563375 Msa0525920:CDS 55.0%
GGAGTCTCGAGAGCAACAAC+GGG - chr3_4:79563399-79563418 None:intergenic 55.0%
GGGAGTCTCGAGAGCAACAA+CGG - chr3_4:79563400-79563419 None:intergenic 55.0%
CCGACAGCTGGAACGGTATT+TGG + chr3_4:79563419-79563438 Msa0525920:CDS 55.0%
!! CTCGTCTTGATTCGGTTCGG+AGG - chr3_4:79563788-79563807 None:intergenic 55.0%
! GTCCGGTCTCTTGGTGATCC+TGG - chr3_4:79563298-79563317 None:intergenic 60.0%
ATGGTGTCCCGATCTCTCGG+TGG + chr3_4:79563359-79563378 Msa0525920:CDS 60.0%
AGACTCCCCGACAGCTGGAA+CGG + chr3_4:79563412-79563431 Msa0525920:CDS 60.0%
TCTCGAGACTCCCCGACAGC+TGG + chr3_4:79563407-79563426 Msa0525920:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 79561274 79564235 79561274 ID=Msa0525920;Name=Msa0525920
chr3_4 mRNA 79561274 79564235 79561274 ID=Msa0525920-mRNA-1;Parent=Msa0525920;Name=Msa0525920-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0|0.2|0|0.66|1|1|6|0|535
chr3_4 exon 79561274 79561330 79561274 ID=Msa0525920-mRNA-1:exon:23836;Parent=Msa0525920-mRNA-1
chr3_4 exon 79561888 79562175 79561888 ID=Msa0525920-mRNA-1:exon:23837;Parent=Msa0525920-mRNA-1
chr3_4 exon 79562613 79562903 79562613 ID=Msa0525920-mRNA-1:exon:23838;Parent=Msa0525920-mRNA-1
chr3_4 exon 79563044 79563193 79563044 ID=Msa0525920-mRNA-1:exon:23839;Parent=Msa0525920-mRNA-1
chr3_4 exon 79563299 79563440 79563299 ID=Msa0525920-mRNA-1:exon:23840;Parent=Msa0525920-mRNA-1
chr3_4 exon 79563556 79564235 79563556 ID=Msa0525920-mRNA-1:exon:23841;Parent=Msa0525920-mRNA-1
chr3_4 CDS 79561274 79561330 79561274 ID=Msa0525920-mRNA-1:cds;Parent=Msa0525920-mRNA-1
chr3_4 CDS 79561888 79562175 79561888 ID=Msa0525920-mRNA-1:cds;Parent=Msa0525920-mRNA-1
chr3_4 CDS 79562613 79562903 79562613 ID=Msa0525920-mRNA-1:cds;Parent=Msa0525920-mRNA-1
chr3_4 CDS 79563044 79563193 79563044 ID=Msa0525920-mRNA-1:cds;Parent=Msa0525920-mRNA-1
chr3_4 CDS 79563299 79563440 79563299 ID=Msa0525920-mRNA-1:cds;Parent=Msa0525920-mRNA-1
chr3_4 CDS 79563556 79564235 79563556 ID=Msa0525920-mRNA-1:cds;Parent=Msa0525920-mRNA-1
Gene Sequence

>Msa0525920

ATGGATTTCAAATTAGTTCTTCATAGGACAACAACCAAATCAGGCAAAAGATCAAAGGAATCACAAAGTGTACATCATGATGAAGTTGTCAAACTTCTATCCAGCCATTCACTTCAAGGCAATGAATTACTCTTGAAAGACACTTTGCCAAATGCTGATTCTCATTTTCAACAGTTGAAACATGAGGTTTCAGAAAAAGATATGGAATCTCAAGAAAAATTGTTATGTCCGCAAAAGTCAGATGGAAGAGATGGAAATGAGTTTGTGAATGATCTGATAGTTACTTCAGAAACTACATTTGATCCCCCAAGTATTGGCAACAACAACAATGGAGAAAATTTCAAGGGTCTTAATGAAGAAAATAAATTGAGCGAAAGTGGATCAACAAATGAGAATCCACAAGATTCTAAGCAAGATCAATGCATAAAGGAACTTCAAGAGTACAAGTCCGACATGGCCAATACATATGATATCACAGTAGGGTCTAATGGAGACTGCAATGGAGAAAAGCATGCAGTACTTGATAATAATGTCCTTGATACTTCTGTGGTTCCTGAAATTGAAGCAGTTCTCTTATTGCAGGATCAACTATTGTTGATCTTGATTGCACACATGACAAGGGAGAGAATCATCATAAATTCTACTTCTTTGTTGCATATCTATAAATACCAGCAGGGAAATGTAGAGCAGACCAAAAGTTTCACGGCCACGAGCATGCTTAAATCAGGTAGGAAGCAAACAAATGACAGCATATACTTTTGTGCCACAATTGACAAGCTTGGCCATGATCACCAAGAGACCGGACACCACATTTCAACCCAAAATCTTAAGGCTTTCGGAGGAGACATGGTGTCCCGATCTCTCGGTGGTTCAGAAACATTAGCCCGTTGTTGCTCTCGAGACTCCCCGACAGCTGGAACGGTATTTGATGAAGAAGCAATTGAGAAAGAAAGGGAAGATTATTCACAACATGAAGAAGCAACTTCACTGATAGTGGAAGAGAAAACAACTTCCACTGAATTAAGTTCAAACAATTCAATATTTGTAAATGGTGGTTATGAAACAAGGGACAGTGTAACAAGGCTAAGCACTGAATCAAATCCTGATAATCCAAACATCACTTGTCAGATGCAAAAATCACCAAGCTTCAATCTCAACCTCCGAACCGAATCAAGACGAGAAGAATCGGATCAAATTCCATTGCTTGATAAGTCTTCAGATGATAGCTTGTCAAACAAGGCAAGTCTAAATATCAGTAACTCAGTGCCTCATGATGAGTATGGTCTCATTGGAGAGAAAATAGTTACTATGGAAAGAAGTTACTCTGAGATATCTAAAGCTTCATTCATTGGTTTCTTAAAACAAGAAGAAGAAGCTCCTGTTCTAGTCATGGCACAGACACAAGATAACAATGCTGGTTCAAAAATGGAAGTGAAGAAAGTGTCATCTATTTCACCTAAAGGAAAGAAAAGCGCAAATTGCGGTCTTACTTCTTTACTAGCTGCATATGTTGTGCAACTGTGCCAAATTAAAACCTTTAGAATTACACATTTTTGTGTTGTGAGTTTCTTTCTGATTTATATTTCTGGAAGTGAAGTTTTGATGTGA
Protein sequence

>Msa0525920

MDFKLVLHRTTTKSGKRSKESQSVHHDEVVKLLSSHSLQGNELLLKDTLPNADSHFQQLKHEVSEKDMESQEKLLCPQKSDGRDGNEFVNDLIVTSETTFDPPSIGNNNNGENFKGLNEENKLSESGSTNENPQDSKQDQCIKELQEYKSDMANTYDITVGSNGDCNGEKHAVLDNNVLDTSVVPEIEAVLLLQDQLLLILIAHMTRERIIINSTSLLHIYKYQQGNVEQTKSFTATSMLKSGRKQTNDSIYFCATIDKLGHDHQETGHHISTQNLKAFGGDMVSRSLGGSETLARCCSRDSPTAGTVFDEEAIEKEREDYSQHEEATSLIVEEKTTSTELSSNNSIFVNGGYETRDSVTRLSTESNPDNPNITCQMQKSPSFNLNLRTESRREESDQIPLLDKSSDDSLSNKASLNISNSVPHDEYGLIGEKIVTMERSYSEISKASFIGFLKQEEEAPVLVMAQTQDNNAGSKMEVKKVSSISPKGKKSANCGLTSLLAAYVVQLCQIKTFRITHFCVVSFFLIYISGSEVLM*