AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0529660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0529660 MtrunA17_Chr4g0000521 98.438 128 2 0 1 128 164 291 2.56e-88 258
Msa0529660 MtrunA17_Chr8g0341911 42.308 78 45 0 45 122 183 260 4.20e-14 66.6
Msa0529660 MtrunA17_Chr5g0401861 40.449 89 48 2 34 121 244 328 5.91e-14 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0529660 AT3G21215.3 89.583 96 10 0 30 125 216 311 1.41e-60 189
Msa0529660 AT3G21215.2 91.304 92 8 0 30 121 216 307 5.55e-58 182
Msa0529660 AT3G21215.1 91.304 92 8 0 30 121 216 307 5.55e-58 182
Msa0529660 AT3G21215.4 91.304 92 8 0 30 121 216 307 5.55e-58 182
Msa0529660 AT2G42240.4 38.983 118 61 3 10 125 176 284 3.55e-15 70.5
Msa0529660 AT3G13700.2 43.421 76 43 0 46 121 204 279 4.27e-14 67.4
Msa0529660 AT2G42240.1 37.719 114 60 3 10 121 176 280 1.91e-13 65.5
Msa0529660 AT3G13700.1 42.667 75 43 0 46 120 222 296 4.37e-13 64.7
Msa0529660 AT2G42240.3 37.168 113 60 3 10 120 176 279 1.65e-12 62.8

Find 130 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTGTATACATTGGTATTT+AGG 0.097707 4_1:+3392759 Msa0529660:three_prime_UTR
CCTTGCAACACCCTGTTTAT+TGG 0.156343 4_1:+3391478 Msa0529660:CDS
ATCGGTGATTAGAAAATTAA+TGG 0.209037 4_1:+3393029 Msa0529660:three_prime_UTR
AGAAAATTAATGGTTGAAAT+TGG 0.211039 4_1:+3393039 Msa0529660:three_prime_UTR
CTCATACTTTGCATTCAATT+TGG 0.213545 4_1:+3392252 Msa0529660:three_prime_UTR
CTTGCAACACCCTGTTTATT+GGG 0.228971 4_1:+3391479 Msa0529660:CDS
TTGAAGTTAAAACTATTTAA+AGG 0.234294 4_1:+3393231 Msa0529660:three_prime_UTR
TGGTATCTGCTTCCAACTTT+AGG 0.243384 4_1:+3392803 Msa0529660:three_prime_UTR
AGGTAAACTACCATCAAATT+TGG 0.247222 4_1:+3393379 Msa0529660:three_prime_UTR
TGGTTTCTCCATTCTGTTGT+AGG 0.248963 4_1:+3392289 Msa0529660:three_prime_UTR
AACCAATATTACTACATTAT+AGG 0.249534 4_1:+3392480 Msa0529660:three_prime_UTR
TCTGTATACATTGGTATTTA+GGG 0.267237 4_1:+3392760 Msa0529660:three_prime_UTR
TGCTACTGCCATATTGTTGC+AGG 0.267857 4_1:+3393335 Msa0529660:three_prime_UTR
CAAATTTAGTTTATGTGAAC+TGG 0.274557 4_1:+3392972 Msa0529660:three_prime_UTR
AAAGCCATTCTCACCTGAAT+TGG 0.289777 4_1:-3390420 None:intergenic
AACTAAGTATTGGTAAATTC+AGG 0.292403 4_1:+3392149 Msa0529660:three_prime_UTR
TGAAGCAGCCATTGAATTAT+AGG 0.297235 4_1:+3393426 Msa0529660:three_prime_UTR
AAACACCTATGAAACTAATC+TGG 0.306027 4_1:-3392660 None:intergenic
CACCCTGTTTATTGGGAACT+TGG 0.308237 4_1:+3391486 Msa0529660:CDS
TAGCAGTTATGTTCCAATTC+AGG 0.309823 4_1:+3390407 Msa0529660:CDS
TTAAGCAAATGAAGATTATA+AGG 0.318083 4_1:+3391671 Msa0529660:CDS
GTACTTTAGGCGATAAAATA+TGG 0.318453 4_1:+3392999 Msa0529660:three_prime_UTR
TATCAGAAATTCGTAGTCAT+AGG 0.333923 4_1:-3393452 None:intergenic
ATCCTATAATGTAGTAATAT+TGG 0.336175 4_1:-3392482 None:intergenic
TTTGTTTGAAATGTGCTTTA+TGG 0.336993 4_1:+3392725 Msa0529660:three_prime_UTR
ATGAGAGTGGAAAGGGTAGA+TGG 0.339157 4_1:-3392909 None:intergenic
AATTTACCAATACTTAGTTT+TGG 0.340502 4_1:-3392196 None:intergenic
AATTTACCAATACTTAGTTT+TGG 0.341755 4_1:-3392145 None:intergenic
CTCCCAAGTTCCCAATAAAC+AGG 0.342043 4_1:-3391489 None:intergenic
TTCTCTATTAACCCAGCATC+TGG 0.347556 4_1:+3391977 Msa0529660:three_prime_UTR
GGTGCTGTTATCCCAAGTTC+TGG 0.347584 4_1:+3391864 Msa0529660:CDS
GACAAAGCTTCCTTTCATAA+TGG 0.348216 4_1:+3393300 Msa0529660:three_prime_UTR
AATATCAGACATGCATTTAT+CGG 0.357969 4_1:+3392621 Msa0529660:three_prime_UTR
GACGTTCCAACCAAATTTGA+TGG 0.366859 4_1:-3393389 None:intergenic
AGCAGATACCAATGCCTGTT+TGG 0.374337 4_1:-3392791 None:intergenic
CTAAGTATTGGTAAATTCAA+TGG 0.374747 4_1:+3392202 Msa0529660:three_prime_UTR
ATATATTGTCTGATTTATAC+AGG 0.374769 4_1:+3393267 Msa0529660:three_prime_UTR
GGTTCCCAGATTAGTTTCAT+AGG 0.376752 4_1:+3392655 Msa0529660:three_prime_UTR
ATCCCAAGTTCTGGTTCAAT+TGG 0.381310 4_1:+3391873 Msa0529660:CDS
TAGTTTCATAGGTGTTTAGA+TGG 0.388607 4_1:+3392666 Msa0529660:three_prime_UTR
ACCTACCCTATGGCTGTTCC+TGG 0.389519 4_1:+3392387 Msa0529660:three_prime_UTR
CAATATGGCAGTAGCAGAAA+TGG 0.389651 4_1:-3393328 None:intergenic
GGCATTGGTACTTGTGCAAC+AGG 0.393304 4_1:-3390363 None:intergenic
ATAAAATATGGTGTTATAAT+CGG 0.398468 4_1:+3393011 Msa0529660:three_prime_UTR
TGGCATCATATACATTCATT+TGG 0.402096 4_1:+3392586 Msa0529660:three_prime_UTR
ATGCCAATTGAACCAGAACT+TGG 0.417682 4_1:-3391876 None:intergenic
GACAAAATACAAGTTATATT+CGG 0.418851 4_1:-3393120 None:intergenic
ATTAATGCTGTGTGATCAAA+TGG 0.429021 4_1:-3393198 None:intergenic
CAGTCATAAACCCCAGATGC+TGG 0.438126 4_1:-3391989 None:intergenic
AAACTATTTAAAGGATTCTC+CGG 0.439864 4_1:+3393240 Msa0529660:three_prime_UTR
TCAATGCCAAAACTAAGTAT+TGG 0.446988 4_1:+3392139 Msa0529660:three_prime_UTR
TCAATGCCAAAACTAAGTAT+TGG 0.446988 4_1:+3392190 Msa0529660:three_prime_UTR
TAGATGGCGTTATCATTTGT+AGG 0.452371 4_1:+3392682 Msa0529660:three_prime_UTR
ACCCTGTTTATTGGGAACTT+GGG 0.453911 4_1:+3391487 Msa0529660:CDS
TTAGGGATGCTTATCCAAAC+AGG 0.455990 4_1:+3392777 Msa0529660:three_prime_UTR
GGATCATACGGAGGAGGAGG+TGG 0.460265 4_1:-3390327 None:intergenic
CATTGCTACTGGAGCTCCAT+TGG 0.465712 4_1:-3392412 None:intergenic
TCATACGGAGGAGGAGGTGG+TGG 0.465780 4_1:-3390324 None:intergenic
CAGCATCTGGGGTTTATGAC+TGG 0.465788 4_1:+3391990 Msa0529660:three_prime_UTR
AATTTGGTTGGAACGTCTGT+TGG 0.467457 4_1:+3393395 Msa0529660:three_prime_UTR
ATCTTCATTTGCTTAAAGCC+AGG 0.474830 4_1:-3391664 None:intergenic
AAACTACCATCAAATTTGGT+TGG 0.478622 4_1:+3393383 Msa0529660:three_prime_UTR
ATGACTTATCAGTAGCTTGA+AGG 0.479145 4_1:+3392432 Msa0529660:three_prime_UTR
GCAACAGGATAGCCTGCATA+TGG 0.482662 4_1:-3390348 None:intergenic
ATGGCTGTTCCTGGTGCCAA+TGG 0.485478 4_1:+3392396 Msa0529660:three_prime_UTR
CATTTATCGGCAGCATGTGT+TGG 0.485795 4_1:+3392634 Msa0529660:three_prime_UTR
TACTGATAAGTCATTGCTAC+TGG 0.489556 4_1:-3392423 None:intergenic
ACTAAGTATTGGTAAATTCA+GGG 0.498341 4_1:+3392150 Msa0529660:three_prime_UTR
ACAACACATGAACCTAAAGT+TGG 0.498571 4_1:-3392815 None:intergenic
TCTCTATTAACCCAGCATCT+GGG 0.500261 4_1:+3391978 Msa0529660:three_prime_UTR
GCAAATGAAGATTATAAGGC+AGG 0.500307 4_1:+3391675 Msa0529660:CDS
AATGATAATGAGAGTGGAAA+GGG 0.506100 4_1:-3392916 None:intergenic
ATGCTTATCCAAACAGGCAT+TGG 0.507705 4_1:+3392783 Msa0529660:three_prime_UTR
GGCGATGACTTGATAATCAA+TGG 0.515826 4_1:-3392529 None:intergenic
AAATTTAGTTTATGTGAACT+GGG 0.516314 4_1:+3392973 Msa0529660:three_prime_UTR
ATTTGGTATAAAACTGCTTG+TGG 0.517696 4_1:+3392269 Msa0529660:three_prime_UTR
AAATGTGCACCACAATCTGC+AGG 0.517804 4_1:+3391842 Msa0529660:CDS
GCTTTGTAATGCAGACAACC+TGG 0.519392 4_1:+3391646 Msa0529660:intron
CTCTATTAACCCAGCATCTG+GGG 0.521963 4_1:+3391979 Msa0529660:three_prime_UTR
ATCAGAAATTCGTAGTCATA+GGG 0.525268 4_1:-3393451 None:intergenic
GGTGAGATAGACACAGGAGC+AGG 0.526097 4_1:-3390384 None:intergenic
TCTCCATTCTGTTGTAGGCA+AGG 0.527422 4_1:+3392294 Msa0529660:three_prime_UTR
TTGGCACCAGGAACAGCCAT+AGG 0.533854 4_1:-3392393 None:intergenic
TGACTTATCAGTAGCTTGAA+GGG 0.534994 4_1:+3392433 Msa0529660:three_prime_UTR
CAAATGGAGTCATGAATGCC+AGG 0.537483 4_1:-3393182 None:intergenic
GTCTTCTAAAGTACACTCCC+AGG 0.538418 4_1:+3393140 Msa0529660:three_prime_UTR
ATAGGGCACCTATAATTCAA+TGG 0.545368 4_1:-3393434 None:intergenic
AAGCTTCCTTTCATAATGGA+AGG 0.546475 4_1:+3393304 Msa0529660:three_prime_UTR
TAGCCTGCATATGGATCATA+CGG 0.548541 4_1:-3390339 None:intergenic
TAAACAGGGTGTTGCAAGGA+GGG 0.548723 4_1:-3391474 None:intergenic
AACATGAGCAGTGATGATAA+TGG 0.551137 4_1:-3392550 None:intergenic
AGCATCTGGGGTTTATGACT+GGG 0.552350 4_1:+3391991 Msa0529660:three_prime_UTR
CCTCCGTATGATCCATATGC+AGG 0.559113 4_1:+3390336 Msa0529660:CDS
ATTCCTTGCCTACAACAGAA+TGG 0.560024 4_1:-3392297 None:intergenic
AAAAGATGGAACCTACCCTA+TGG 0.564455 4_1:+3392377 Msa0529660:three_prime_UTR
AACACCTATGAAACTAATCT+GGG 0.566767 4_1:-3392659 None:intergenic
TAACAGCACCCTGCAGATTG+TGG 0.570755 4_1:-3391851 None:intergenic
CATTAATGAGGAAGAAGTGA+GGG 0.575202 4_1:+3391516 Msa0529660:CDS
AATGGACCTTCCATTATGAA+AGG 0.578687 4_1:-3393310 None:intergenic
AGTCATAAACCCCAGATGCT+GGG 0.579970 4_1:-3391988 None:intergenic
TATGGATCATACGGAGGAGG+AGG 0.580137 4_1:-3390330 None:intergenic
ATAGACACAGGAGCAGGCAT+TGG 0.580929 4_1:-3390378 None:intergenic
CAATGATAATGAGAGTGGAA+AGG 0.592019 4_1:-3392917 None:intergenic
AATGACAATGATAATGAGAG+TGG 0.596480 4_1:-3392922 None:intergenic
AATCAGACAATATATAACTC+CGG 0.598244 4_1:-3393259 None:intergenic
TGAATTGGAACATAACTGCT+AGG 0.602093 4_1:-3390405 None:intergenic
ATTTAGTTTATGTGAACTGG+GGG 0.602858 4_1:+3392975 Msa0529660:three_prime_UTR
CCAATAAACAGGGTGTTGCA+AGG 0.603873 4_1:-3391478 None:intergenic
ACTGGAGCTCCATTGGCACC+AGG 0.605650 4_1:-3392405 None:intergenic
CTTGGGAGAGAACATTAATG+AGG 0.606113 4_1:+3391504 Msa0529660:CDS
GCATATGGATCATACGGAGG+AGG 0.606969 4_1:-3390333 None:intergenic
TGAAGATTATAAGGCAGGAG+AGG 0.609049 4_1:+3391680 Msa0529660:CDS
TGCCAATTGAACCAGAACTT+GGG 0.610282 4_1:-3391875 None:intergenic
AATTTAGTTTATGTGAACTG+GGG 0.610923 4_1:+3392974 Msa0529660:three_prime_UTR
GACTTATCAGTAGCTTGAAG+GGG 0.615446 4_1:+3392434 Msa0529660:three_prime_UTR
TGGCACCAGGAACAGCCATA+GGG 0.617677 4_1:-3392392 None:intergenic
TATATTGTCTGATTTATACA+GGG 0.645169 4_1:+3393268 Msa0529660:three_prime_UTR
ACATTAATGAGGAAGAAGTG+AGG 0.646735 4_1:+3391515 Msa0529660:CDS
AAACAAATGAAAGAAACTCA+CGG 0.653052 4_1:-3392103 None:intergenic
AAATGGAGTCATGAATGCCA+GGG 0.656673 4_1:-3393181 None:intergenic
TCCCAAGTTCCCAATAAACA+GGG 0.668529 4_1:-3391488 None:intergenic
GTTCTGGTTCAATTGGCATG+CGG 0.674324 4_1:+3391880 Msa0529660:CDS
AATGTGCACCACAATCTGCA+GGG 0.681242 4_1:+3391843 Msa0529660:CDS
TGGAGTCATGAATGCCAGGG+TGG 0.694151 4_1:-3393178 None:intergenic
CTGCTAGGTGAGATAGACAC+AGG 0.695547 4_1:-3390390 None:intergenic
ATAAACAGGGTGTTGCAAGG+AGG 0.695618 4_1:-3391475 None:intergenic
ACCAGGAACAGCCATAGGGT+AGG 0.709355 4_1:-3392388 None:intergenic
ATTAATGAGGAAGAAGTGAG+GGG 0.716298 4_1:+3391517 Msa0529660:CDS
CCTGCATATGGATCATACGG+AGG 0.734736 4_1:-3390336 None:intergenic
TCATGTTAATGAATGCACAG+TGG 0.853491 4_1:+3392566 Msa0529660:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAAAATATGATGTTTAAA+GGG + chr4_1:3391258-3391277 Msa0529660:intron 15.0%
!! TCATTGATAAACAATATTAA+CGG - chr4_1:3391397-3391416 None:intergenic 15.0%
!! ATAAACTAAATTTGAAAAAC+AGG - chr4_1:3392966-3392985 None:intergenic 15.0%
!!! ATAAAATATGGTGTTATAAT+CGG + chr4_1:3393011-3393030 Msa0529660:three_prime_UTR 15.0%
!! TTGAAGTTAAAACTATTTAA+AGG + chr4_1:3393231-3393250 Msa0529660:three_prime_UTR 15.0%
!! GGAAAAAATATGATGTTTAA+AGG + chr4_1:3391257-3391276 Msa0529660:intron 20.0%
!! TTAAGCAAATGAAGATTATA+AGG + chr4_1:3391671-3391690 Msa0529660:CDS 20.0%
!!! TACAATATCCTTTTTTATTG+TGG + chr4_1:3391904-3391923 Msa0529660:CDS 20.0%
!!! TCTTTCATTTGTTTTTTCAA+TGG + chr4_1:3392112-3392131 Msa0529660:three_prime_UTR 20.0%
!!! AATTTACCAATACTTAGTTT+TGG - chr4_1:3392148-3392167 None:intergenic 20.0%
!!! TTTTGGCATTGAAAAATATA+AGG - chr4_1:3392182-3392201 None:intergenic 20.0%
!!! AATTTACCAATACTTAGTTT+TGG - chr4_1:3392148-3392167 None:intergenic 20.0%
!! AACCAATATTACTACATTAT+AGG + chr4_1:3392480-3392499 Msa0529660:three_prime_UTR 20.0%
!! ATCCTATAATGTAGTAATAT+TGG - chr4_1:3392485-3392504 None:intergenic 20.0%
!!! AAATTTAGTTTATGTGAACT+GGG + chr4_1:3392973-3392992 Msa0529660:three_prime_UTR 20.0%
!! AGAAAATTAATGGTTGAAAT+TGG + chr4_1:3393039-3393058 Msa0529660:three_prime_UTR 20.0%
!! GACAAAATACAAGTTATATT+CGG - chr4_1:3393123-3393142 None:intergenic 20.0%
!! ATATATTGTCTGATTTATAC+AGG + chr4_1:3393267-3393286 Msa0529660:three_prime_UTR 20.0%
!! TATATTGTCTGATTTATACA+GGG + chr4_1:3393268-3393287 Msa0529660:three_prime_UTR 20.0%
!! TTAAACTGTTAATGCTACTT+TGG + chr4_1:3390441-3390460 Msa0529660:intron 25.0%
! AAAGTTGGAGTAATTATTGA+GGG - chr4_1:3390717-3390736 None:intergenic 25.0%
! AAAAGTTGGAGTAATTATTG+AGG - chr4_1:3390718-3390737 None:intergenic 25.0%
! TGTCTTTCTCTAATAAAGTA+AGG + chr4_1:3390768-3390787 Msa0529660:intron 25.0%
! GTCTTTCTCTAATAAAGTAA+GGG + chr4_1:3390769-3390788 Msa0529660:intron 25.0%
! ATCAATTTCTAACAGTTGAA+TGG - chr4_1:3390954-3390973 None:intergenic 25.0%
! ATTGTATCTCAAAGTGAATA+TGG + chr4_1:3391049-3391068 Msa0529660:intron 25.0%
! TTGTATCTCAAAGTGAATAT+GGG + chr4_1:3391050-3391069 Msa0529660:intron 25.0%
!!! TCTATTTTAACTACCAAAGA+AGG + chr4_1:3391166-3391185 Msa0529660:intron 25.0%
!!! AACATCATATTTTTTCCAGT+AGG - chr4_1:3391254-3391273 None:intergenic 25.0%
! GATTAATCCAATATATGACA+CGG - chr4_1:3391615-3391634 None:intergenic 25.0%
! ATGAAAAATACATGGTAAGT+TGG - chr4_1:3392069-3392088 None:intergenic 25.0%
! TATCAGTGATGAAAAATACA+TGG - chr4_1:3392077-3392096 None:intergenic 25.0%
! AAACAAATGAAAGAAACTCA+CGG - chr4_1:3392106-3392125 None:intergenic 25.0%
!!! AACTAAGTATTGGTAAATTC+AGG + chr4_1:3392149-3392168 Msa0529660:three_prime_UTR 25.0%
!!! ACTAAGTATTGGTAAATTCA+GGG + chr4_1:3392150-3392169 Msa0529660:three_prime_UTR 25.0%
!!! CTAAGTATTGGTAAATTCAA+TGG + chr4_1:3392202-3392221 Msa0529660:three_prime_UTR 25.0%
! AGATATTCCAAAAATCCATT+TGG + chr4_1:3392348-3392367 Msa0529660:three_prime_UTR 25.0%
!!! TCTTTTTCCAAATGGATTTT+TGG - chr4_1:3392358-3392377 None:intergenic 25.0%
! AATATCAGACATGCATTTAT+CGG + chr4_1:3392621-3392640 Msa0529660:three_prime_UTR 25.0%
!! TTTGTTTGAAATGTGCTTTA+TGG + chr4_1:3392725-3392744 Msa0529660:three_prime_UTR 25.0%
!! TTAGACTTTTCTGTATACAT+TGG + chr4_1:3392751-3392770 Msa0529660:three_prime_UTR 25.0%
! TTCTGTATACATTGGTATTT+AGG + chr4_1:3392759-3392778 Msa0529660:three_prime_UTR 25.0%
! TCTGTATACATTGGTATTTA+GGG + chr4_1:3392760-3392779 Msa0529660:three_prime_UTR 25.0%
!!! TGATGCTTTCTTTATTTTCA+TGG + chr4_1:3392861-3392880 Msa0529660:three_prime_UTR 25.0%
! AACTAAATTTGAAAAACAGG+AGG - chr4_1:3392963-3392982 None:intergenic 25.0%
!! CAAATTTAGTTTATGTGAAC+TGG + chr4_1:3392972-3392991 Msa0529660:three_prime_UTR 25.0%
!! AATTTAGTTTATGTGAACTG+GGG + chr4_1:3392974-3392993 Msa0529660:three_prime_UTR 25.0%
! ATCGGTGATTAGAAAATTAA+TGG + chr4_1:3393029-3393048 Msa0529660:three_prime_UTR 25.0%
! AAACTATTTAAAGGATTCTC+CGG + chr4_1:3393240-3393259 Msa0529660:three_prime_UTR 25.0%
! AATCAGACAATATATAACTC+CGG - chr4_1:3393262-3393281 None:intergenic 25.0%
GCTCGTGATTTATAAAGATA+AGG + chr4_1:3390520-3390539 Msa0529660:intron 30.0%
CGTGATTTATAAAGATAAGG+CGG + chr4_1:3390523-3390542 Msa0529660:intron 30.0%
ATTACAGAGCTTATAGCTAA+CGG - chr4_1:3390563-3390582 None:intergenic 30.0%
AAGTGACACTCATTATGAAA+CGG - chr4_1:3390617-3390636 None:intergenic 30.0%
!! TTCATAATGAGTGTCACTTT+AGG + chr4_1:3390618-3390637 Msa0529660:intron 30.0%
!! TCATAATGAGTGTCACTTTA+GGG + chr4_1:3390619-3390638 Msa0529660:intron 30.0%
! GAAAATTCTGAGAGAAAAGT+TGG - chr4_1:3390732-3390751 None:intergenic 30.0%
TTGGCACATAGATTAAGAAA+TGG - chr4_1:3390834-3390853 None:intergenic 30.0%
TCTTAATCTATGTGCCAAAA+TGG + chr4_1:3390836-3390855 Msa0529660:intron 30.0%
!!! AGCATTTGTCTTTGTTTTGT+CGG + chr4_1:3391079-3391098 Msa0529660:intron 30.0%
CTTAATTGCTATATTCTGCT+TGG + chr4_1:3391114-3391133 Msa0529660:intron 30.0%
ACATTCATTCAGCAATTTAG+AGG - chr4_1:3391212-3391231 None:intergenic 30.0%
GTTCGAAGTATATTCATAGT+TGG + chr4_1:3391300-3391319 Msa0529660:intron 30.0%
TCACTGCATGATAATAACTA+AGG - chr4_1:3391331-3391350 None:intergenic 30.0%
! GTTTTACATACACACTGAAA+AGG - chr4_1:3391543-3391562 None:intergenic 30.0%
TTGGATTAATCTGTTGAACT+TGG + chr4_1:3391624-3391643 Msa0529660:intron 30.0%
! TATTTGTGCCTTTTATTGTC+TGG + chr4_1:3391748-3391767 Msa0529660:intron 30.0%
GAAAAGATCCAGACAATAAA+AGG - chr4_1:3391759-3391778 None:intergenic 30.0%
CTTATGTTTACGCTAATACT+TGG + chr4_1:3391782-3391801 Msa0529660:intron 30.0%
! GTATTTTTCATCACTGATAC+TGG + chr4_1:3392078-3392097 Msa0529660:three_prime_UTR 30.0%
TCAATGCCAAAACTAAGTAT+TGG + chr4_1:3392139-3392158 Msa0529660:three_prime_UTR 30.0%
TCAATGCCAAAACTAAGTAT+TGG + chr4_1:3392139-3392158 Msa0529660:three_prime_UTR 30.0%
CTCATACTTTGCATTCAATT+TGG + chr4_1:3392252-3392271 Msa0529660:three_prime_UTR 30.0%
ATTTGGTATAAAACTGCTTG+TGG + chr4_1:3392269-3392288 Msa0529660:three_prime_UTR 30.0%
! CCATCTTTTCTTTTTCCAAA+TGG - chr4_1:3392366-3392385 None:intergenic 30.0%
CCATTTGGAAAAAGAAAAGA+TGG + chr4_1:3392363-3392382 Msa0529660:three_prime_UTR 30.0%
TGGCATCATATACATTCATT+TGG + chr4_1:3392586-3392605 Msa0529660:three_prime_UTR 30.0%
AACACCTATGAAACTAATCT+GGG - chr4_1:3392662-3392681 None:intergenic 30.0%
AAACACCTATGAAACTAATC+TGG - chr4_1:3392663-3392682 None:intergenic 30.0%
TAGTTTCATAGGTGTTTAGA+TGG + chr4_1:3392666-3392685 Msa0529660:three_prime_UTR 30.0%
!!! CTTTCTTTATTTTCATGGCA+TGG + chr4_1:3392866-3392885 Msa0529660:three_prime_UTR 30.0%
! AATGATAATGAGAGTGGAAA+GGG - chr4_1:3392919-3392938 None:intergenic 30.0%
! AATGACAATGATAATGAGAG+TGG - chr4_1:3392925-3392944 None:intergenic 30.0%
! ATTTAGTTTATGTGAACTGG+GGG + chr4_1:3392975-3392994 Msa0529660:three_prime_UTR 30.0%
GTACTTTAGGCGATAAAATA+TGG + chr4_1:3392999-3393018 Msa0529660:three_prime_UTR 30.0%
ATTAATGCTGTGTGATCAAA+TGG - chr4_1:3393201-3393220 None:intergenic 30.0%
AAAAATCACCTGCAACAATA+TGG - chr4_1:3393346-3393365 None:intergenic 30.0%
AGGTAAACTACCATCAAATT+TGG + chr4_1:3393379-3393398 Msa0529660:three_prime_UTR 30.0%
AAACTACCATCAAATTTGGT+TGG + chr4_1:3393383-3393402 Msa0529660:three_prime_UTR 30.0%
ATCAGAAATTCGTAGTCATA+GGG - chr4_1:3393454-3393473 None:intergenic 30.0%
TATCAGAAATTCGTAGTCAT+AGG - chr4_1:3393455-3393474 None:intergenic 30.0%
TGAATTGGAACATAACTGCT+AGG - chr4_1:3390408-3390427 None:intergenic 35.0%
TAGCAGTTATGTTCCAATTC+AGG + chr4_1:3390407-3390426 Msa0529660:CDS 35.0%
GTAATTATTGAGGGCAAAGT+TGG - chr4_1:3390708-3390727 None:intergenic 35.0%
TAATCTATGTGCCAAAATGG+AGG + chr4_1:3390839-3390858 Msa0529660:intron 35.0%
AATCTATGTGCCAAAATGGA+GGG + chr4_1:3390840-3390859 Msa0529660:intron 35.0%
!! TTCACAATGAGTGTCGTTTA+AGG - chr4_1:3390876-3390895 None:intergenic 35.0%
AAACGACACTCATTGTGAAA+TGG + chr4_1:3390877-3390896 Msa0529660:intron 35.0%
! GGCTTGATATTTTCTCTTGT+TGG + chr4_1:3390917-3390936 Msa0529660:intron 35.0%
TATCTCAAAGTGAATATGGG+AGG + chr4_1:3391053-3391072 Msa0529660:intron 35.0%
ATCTCAAAGTGAATATGGGA+GGG + chr4_1:3391054-3391073 Msa0529660:intron 35.0%
TGCTTTAATCTTGCCTTCTT+TGG - chr4_1:3391182-3391201 None:intergenic 35.0%
ATGTGTTATGTACATCCTAC+TGG + chr4_1:3391236-3391255 Msa0529660:intron 35.0%
ACATTAATGAGGAAGAAGTG+AGG + chr4_1:3391515-3391534 Msa0529660:CDS 35.0%
CATTAATGAGGAAGAAGTGA+GGG + chr4_1:3391516-3391535 Msa0529660:CDS 35.0%
ATTAATGAGGAAGAAGTGAG+GGG + chr4_1:3391517-3391536 Msa0529660:CDS 35.0%
TTCTATGCCGTGTCATATAT+TGG + chr4_1:3391605-3391624 Msa0529660:intron 35.0%
ATCTTCATTTGCTTAAAGCC+AGG - chr4_1:3391667-3391686 None:intergenic 35.0%
GCAAATGAAGATTATAAGGC+AGG + chr4_1:3391675-3391694 Msa0529660:CDS 35.0%
CAGATGCTCCACAATAAAAA+AGG - chr4_1:3391915-3391934 None:intergenic 35.0%
TACTGATAAGTCATTGCTAC+TGG - chr4_1:3392426-3392445 None:intergenic 35.0%
ATGACTTATCAGTAGCTTGA+AGG + chr4_1:3392432-3392451 Msa0529660:three_prime_UTR 35.0%
TGACTTATCAGTAGCTTGAA+GGG + chr4_1:3392433-3392452 Msa0529660:three_prime_UTR 35.0%
AACATGAGCAGTGATGATAA+TGG - chr4_1:3392553-3392572 None:intergenic 35.0%
TCATGTTAATGAATGCACAG+TGG + chr4_1:3392566-3392585 Msa0529660:three_prime_UTR 35.0%
TAGATGGCGTTATCATTTGT+AGG + chr4_1:3392682-3392701 Msa0529660:three_prime_UTR 35.0%
ACAACACATGAACCTAAAGT+TGG - chr4_1:3392818-3392837 None:intergenic 35.0%
! CAATGATAATGAGAGTGGAA+AGG - chr4_1:3392920-3392939 None:intergenic 35.0%
AAAACAGGAGGCTAAAAAAG+TGG - chr4_1:3392951-3392970 None:intergenic 35.0%
! GGATCTAGTTTAAAAAGCCT+GGG - chr4_1:3393160-3393179 None:intergenic 35.0%
! TGGATCTAGTTTAAAAAGCC+TGG - chr4_1:3393161-3393180 None:intergenic 35.0%
! TTTTTAAACTAGATCCACCC+TGG + chr4_1:3393164-3393183 Msa0529660:three_prime_UTR 35.0%
GACAAAGCTTCCTTTCATAA+TGG + chr4_1:3393300-3393319 Msa0529660:three_prime_UTR 35.0%
! AAGCTTCCTTTCATAATGGA+AGG + chr4_1:3393304-3393323 Msa0529660:three_prime_UTR 35.0%
AATGGACCTTCCATTATGAA+AGG - chr4_1:3393313-3393332 None:intergenic 35.0%
TGAAGCAGCCATTGAATTAT+AGG + chr4_1:3393426-3393445 Msa0529660:three_prime_UTR 35.0%
! ATAGGGCACCTATAATTCAA+TGG - chr4_1:3393437-3393456 None:intergenic 35.0%
TAGCCTGCATATGGATCATA+CGG - chr4_1:3390342-3390361 None:intergenic 40.0%
TGTTCCAATTCAGGTGAGAA+TGG + chr4_1:3390416-3390435 Msa0529660:intron 40.0%
AAAGCCATTCTCACCTGAAT+TGG - chr4_1:3390423-3390442 None:intergenic 40.0%
GACACTCATTGTGAAATGGA+GGG + chr4_1:3390881-3390900 Msa0529660:intron 40.0%
AATGGAGGGAGTAGTTGAAA+AGG + chr4_1:3390895-3390914 Msa0529660:intron 40.0%
ATGGAGGGAGTAGTTGAAAA+GGG + chr4_1:3390896-3390915 Msa0529660:intron 40.0%
TCTCAAAGTGAATATGGGAG+GGG + chr4_1:3391055-3391074 Msa0529660:intron 40.0%
CTTGCAACACCCTGTTTATT+GGG + chr4_1:3391479-3391498 Msa0529660:CDS 40.0%
ACCCTGTTTATTGGGAACTT+GGG + chr4_1:3391487-3391506 Msa0529660:CDS 40.0%
TCCCAAGTTCCCAATAAACA+GGG - chr4_1:3391491-3391510 None:intergenic 40.0%
CTTGGGAGAGAACATTAATG+AGG + chr4_1:3391504-3391523 Msa0529660:CDS 40.0%
TGAAGATTATAAGGCAGGAG+AGG + chr4_1:3391680-3391699 Msa0529660:CDS 40.0%
GGTATCCAATAACCTATTGC+AGG + chr4_1:3391803-3391822 Msa0529660:intron 40.0%
CACATCCTGCAATAGGTTAT+TGG - chr4_1:3391811-3391830 None:intergenic 40.0%
CACTATTCACATCCTGCAAT+AGG - chr4_1:3391818-3391837 None:intergenic 40.0%
!! ATCCCAAGTTCTGGTTCAAT+TGG + chr4_1:3391873-3391892 Msa0529660:CDS 40.0%
TGCCAATTGAACCAGAACTT+GGG - chr4_1:3391878-3391897 None:intergenic 40.0%
ATGCCAATTGAACCAGAACT+TGG - chr4_1:3391879-3391898 None:intergenic 40.0%
TTCTCTATTAACCCAGCATC+TGG + chr4_1:3391977-3391996 Msa0529660:three_prime_UTR 40.0%
TCTCTATTAACCCAGCATCT+GGG + chr4_1:3391978-3391997 Msa0529660:three_prime_UTR 40.0%
TGGTTTCTCCATTCTGTTGT+AGG + chr4_1:3392289-3392308 Msa0529660:three_prime_UTR 40.0%
ATTCCTTGCCTACAACAGAA+TGG - chr4_1:3392300-3392319 None:intergenic 40.0%
AAAAGATGGAACCTACCCTA+TGG + chr4_1:3392377-3392396 Msa0529660:three_prime_UTR 40.0%
GACTTATCAGTAGCTTGAAG+GGG + chr4_1:3392434-3392453 Msa0529660:three_prime_UTR 40.0%
! GGCGATGACTTGATAATCAA+TGG - chr4_1:3392532-3392551 None:intergenic 40.0%
!! GGTTCCCAGATTAGTTTCAT+AGG + chr4_1:3392655-3392674 Msa0529660:three_prime_UTR 40.0%
TTAGGGATGCTTATCCAAAC+AGG + chr4_1:3392777-3392796 Msa0529660:three_prime_UTR 40.0%
ATGCTTATCCAAACAGGCAT+TGG + chr4_1:3392783-3392802 Msa0529660:three_prime_UTR 40.0%
TGGTATCTGCTTCCAACTTT+AGG + chr4_1:3392803-3392822 Msa0529660:three_prime_UTR 40.0%
AAATGGAGTCATGAATGCCA+GGG - chr4_1:3393184-3393203 None:intergenic 40.0%
CAATATGGCAGTAGCAGAAA+TGG - chr4_1:3393331-3393350 None:intergenic 40.0%
!! GCAGGTGATTTTTGTTTGCA+CGG + chr4_1:3393353-3393372 Msa0529660:three_prime_UTR 40.0%
!! CAGGTGATTTTTGTTTGCAC+GGG + chr4_1:3393354-3393373 Msa0529660:three_prime_UTR 40.0%
!! GATTTTTGTTTGCACGGGTA+AGG + chr4_1:3393359-3393378 Msa0529660:three_prime_UTR 40.0%
GACGTTCCAACCAAATTTGA+TGG - chr4_1:3393392-3393411 None:intergenic 40.0%
AATTTGGTTGGAACGTCTGT+TGG + chr4_1:3393395-3393414 Msa0529660:three_prime_UTR 40.0%
GAGCTCAAAATTGCACACGA+AGG - chr4_1:3390504-3390523 None:intergenic 45.0%
! TTGTCTTTCGCCCTCCATTT+TGG - chr4_1:3390853-3390872 None:intergenic 45.0%
CGACACTCATTGTGAAATGG+AGG + chr4_1:3390880-3390899 Msa0529660:intron 45.0%
ACTGTCCTTGAAGTCCTGAA+CGG + chr4_1:3391008-3391027 Msa0529660:intron 45.0%
!! TAAACAGGGTGTTGCAAGGA+GGG - chr4_1:3391477-3391496 None:intergenic 45.0%
!! ATAAACAGGGTGTTGCAAGG+AGG - chr4_1:3391478-3391497 None:intergenic 45.0%
!! CCAATAAACAGGGTGTTGCA+AGG - chr4_1:3391481-3391500 None:intergenic 45.0%
CCTTGCAACACCCTGTTTAT+TGG + chr4_1:3391478-3391497 Msa0529660:CDS 45.0%
CACCCTGTTTATTGGGAACT+TGG + chr4_1:3391486-3391505 Msa0529660:CDS 45.0%
CTCCCAAGTTCCCAATAAAC+AGG - chr4_1:3391492-3391511 None:intergenic 45.0%
GAACAGAAGAGCTGTGTCAA+AGG - chr4_1:3391570-3391589 None:intergenic 45.0%
GCTTTGTAATGCAGACAACC+TGG + chr4_1:3391646-3391665 Msa0529660:intron 45.0%
AAATGTGCACCACAATCTGC+AGG + chr4_1:3391842-3391861 Msa0529660:CDS 45.0%
AATGTGCACCACAATCTGCA+GGG + chr4_1:3391843-3391862 Msa0529660:CDS 45.0%
!! GTTCTGGTTCAATTGGCATG+CGG + chr4_1:3391880-3391899 Msa0529660:CDS 45.0%
CTCTATTAACCCAGCATCTG+GGG + chr4_1:3391979-3391998 Msa0529660:three_prime_UTR 45.0%
AGTCATAAACCCCAGATGCT+GGG - chr4_1:3391991-3392010 None:intergenic 45.0%
! AGCATCTGGGGTTTATGACT+GGG + chr4_1:3391991-3392010 Msa0529660:three_prime_UTR 45.0%
TCTCCATTCTGTTGTAGGCA+AGG + chr4_1:3392294-3392313 Msa0529660:three_prime_UTR 45.0%
CATTTATCGGCAGCATGTGT+TGG + chr4_1:3392634-3392653 Msa0529660:three_prime_UTR 45.0%
AGCAGATACCAATGCCTGTT+TGG - chr4_1:3392794-3392813 None:intergenic 45.0%
ATGAGAGTGGAAAGGGTAGA+TGG - chr4_1:3392912-3392931 None:intergenic 45.0%
!! GTCTTCTAAAGTACACTCCC+AGG + chr4_1:3393140-3393159 Msa0529660:three_prime_UTR 45.0%
CAAATGGAGTCATGAATGCC+AGG - chr4_1:3393185-3393204 None:intergenic 45.0%
TGCTACTGCCATATTGTTGC+AGG + chr4_1:3393335-3393354 Msa0529660:three_prime_UTR 45.0%
TATGGATCATACGGAGGAGG+AGG - chr4_1:3390333-3390352 None:intergenic 50.0%
GCATATGGATCATACGGAGG+AGG - chr4_1:3390336-3390355 None:intergenic 50.0%
CCTGCATATGGATCATACGG+AGG - chr4_1:3390339-3390358 None:intergenic 50.0%
CCTCCGTATGATCCATATGC+AGG + chr4_1:3390336-3390355 Msa0529660:CDS 50.0%
GCAACAGGATAGCCTGCATA+TGG - chr4_1:3390351-3390370 None:intergenic 50.0%
!! GGCATTGGTACTTGTGCAAC+AGG - chr4_1:3390366-3390385 None:intergenic 50.0%
ATAGACACAGGAGCAGGCAT+TGG - chr4_1:3390381-3390400 None:intergenic 50.0%
CTGCTAGGTGAGATAGACAC+AGG - chr4_1:3390393-3390412 None:intergenic 50.0%
CTGTCCTTGAAGTCCTGAAC+GGG + chr4_1:3391009-3391028 Msa0529660:intron 50.0%
TAACAGCACCCTGCAGATTG+TGG - chr4_1:3391854-3391873 None:intergenic 50.0%
!! GGTGCTGTTATCCCAAGTTC+TGG + chr4_1:3391864-3391883 Msa0529660:CDS 50.0%
CAGTCATAAACCCCAGATGC+TGG - chr4_1:3391992-3392011 None:intergenic 50.0%
! CAGCATCTGGGGTTTATGAC+TGG + chr4_1:3391990-3392009 Msa0529660:three_prime_UTR 50.0%
CATTGCTACTGGAGCTCCAT+TGG - chr4_1:3392415-3392434 None:intergenic 50.0%
GGTGAGATAGACACAGGAGC+AGG - chr4_1:3390387-3390406 None:intergenic 55.0%
TGTGCCCGTTCAGGACTTCA+AGG - chr4_1:3391016-3391035 None:intergenic 55.0%
GAACTCTTCTGTGCCCGTTC+AGG - chr4_1:3391025-3391044 None:intergenic 55.0%
ACCTACCCTATGGCTGTTCC+TGG + chr4_1:3392387-3392406 Msa0529660:three_prime_UTR 55.0%
ACCAGGAACAGCCATAGGGT+AGG - chr4_1:3392391-3392410 None:intergenic 55.0%
TGGCACCAGGAACAGCCATA+GGG - chr4_1:3392395-3392414 None:intergenic 55.0%
TTGGCACCAGGAACAGCCAT+AGG - chr4_1:3392396-3392415 None:intergenic 55.0%
! ATGGCTGTTCCTGGTGCCAA+TGG + chr4_1:3392396-3392415 Msa0529660:three_prime_UTR 55.0%
! GTGAACTGGGGGCGTACTTT+AGG + chr4_1:3392986-3393005 Msa0529660:three_prime_UTR 55.0%
TGGAGTCATGAATGCCAGGG+TGG - chr4_1:3393181-3393200 None:intergenic 55.0%
TCATACGGAGGAGGAGGTGG+TGG - chr4_1:3390327-3390346 None:intergenic 60.0%
GGATCATACGGAGGAGGAGG+TGG - chr4_1:3390330-3390349 None:intergenic 60.0%
ACTGGAGCTCCATTGGCACC+AGG - chr4_1:3392408-3392427 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 3390315 3393485 3390315 ID=Msa0529660;Name=Msa0529660
chr4_1 mRNA 3390315 3393485 3390315 ID=Msa0529660-mRNA-1;Parent=Msa0529660;Name=Msa0529660-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|0.66|0.5|1|1|1|4|1550|128
chr4_1 exon 3390315 3390428 3390315 ID=Msa0529660-mRNA-1:exon:940;Parent=Msa0529660-mRNA-1
chr4_1 exon 3391460 3391551 3391460 ID=Msa0529660-mRNA-1:exon:941;Parent=Msa0529660-mRNA-1
chr4_1 exon 3391660 3391729 3391660 ID=Msa0529660-mRNA-1:exon:942;Parent=Msa0529660-mRNA-1
chr4_1 exon 3391825 3393485 3391825 ID=Msa0529660-mRNA-1:exon:943;Parent=Msa0529660-mRNA-1
chr4_1 CDS 3390315 3390428 3390315 ID=Msa0529660-mRNA-1:cds;Parent=Msa0529660-mRNA-1
chr4_1 CDS 3391460 3391551 3391460 ID=Msa0529660-mRNA-1:cds;Parent=Msa0529660-mRNA-1
chr4_1 CDS 3391660 3391729 3391660 ID=Msa0529660-mRNA-1:cds;Parent=Msa0529660-mRNA-1
chr4_1 CDS 3391825 3391935 3391825 ID=Msa0529660-mRNA-1:cds;Parent=Msa0529660-mRNA-1
chr4_1 three_prime_UTR 3391936 3393485 3391936 ID=Msa0529660-mRNA-1:three_prime_utr;Parent=Msa0529660-mRNA-1
Gene Sequence

>Msa0529660

TATATGGCTCCACCACCTCCTCCTCCGTATGATCCATATGCAGGCTATCCTGTTGCACAAGTACCAATGCCTGCTCCTGTGTCTATCTCACCTAGCAGTTATGTTCCAATTCAGAACACTAAAGATAACCCTCCTTGCAACACCCTGTTTATTGGGAACTTGGGAGAGAACATTAATGAGGAAGAAGTGAGGGGCCTTTTCAGTGTACAACCTGGCTTTAAGCAAATGAAGATTATAAGGCAGGAGAGGCATACAGTTTGCTTCATTGAGTTTGAAGATGTGAATAGTGCTACAAATGTGCACCACAATCTGCAGGGTGCTGTTATCCCAAGTTCTGGTTCAATTGGCATGCGGATACAATATCCTTTTTTATTGTGGAGCATCTGA
Protein sequence

>Msa0529660

YMAPPPPPPYDPYAGYPVAQVPMPAPVSISPSSYVPIQNTKDNPPCNTLFIGNLGENINEEEVRGLFSVQPGFKQMKIIRQERHTVCFIEFEDVNSATNVHHNLQGAVIPSSGSIGMRIQYPFLLWSI*