AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0530320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0530320 MtrunA17_Chr4g0003201 97.143 280 7 1 1 279 1 280 0.0 556
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0530320 AT3G15820.1 78.302 212 46 0 46 257 69 280 7.15e-123 353
Msa0530320 AT3G15830.1 70.386 233 68 1 46 278 64 295 7.56e-118 340

Find 92 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTTGTCTGCATTTCATTT+TGG 0.166724 4_1:+4363894 Msa0530320:three_prime_UTR
TGCAAACGAGTTATATATTA+TGG 0.202134 4_1:+4362391 Msa0530320:CDS
TTCTTCTCCACCCTTTGATT+TGG 0.205170 4_1:+4361231 Msa0530320:CDS
ACTACTACTGTTCATTGTTT+TGG 0.215489 4_1:-4360816 None:intergenic
TGTGGTTTGAATATATTTCT+AGG 0.233839 4_1:-4363931 None:intergenic
AAAGGGAAACACACAGAAAA+AGG 0.257363 4_1:+4363577 Msa0530320:three_prime_UTR
TCTTCTCCACCCTTTGATTT+GGG 0.276299 4_1:+4361232 Msa0530320:CDS
GTTAGAGTGATTGGTTTGAT+TGG 0.280580 4_1:-4361026 None:intergenic
TGTGGAATACACGCTCTTTA+TGG 0.285794 4_1:+4361201 Msa0530320:CDS
TTCAAACTCCTCCTCATGTT+TGG 0.314633 4_1:-4363742 None:intergenic
TGCAACCAAATCATGTTTAG+AGG 0.340593 4_1:-4363531 None:intergenic
TTAGAGTGATTGGTTTGATT+GGG 0.342876 4_1:-4361025 None:intergenic
CTTCAGCTTCGTTCATGAAA+TGG 0.346389 4_1:+4361098 Msa0530320:CDS
AGGCCATTACACTATTGATT+TGG 0.346538 4_1:+4363409 Msa0530320:CDS
GAGGGGTTATTTATAGAATT+CGG 0.352448 4_1:-4360902 None:intergenic
TCACCTGGGGCAATGGTAGC+TGG 0.358861 4_1:-4362497 None:intergenic
TACACTATTGATTTGGCTGT+TGG 0.360274 4_1:+4363416 Msa0530320:CDS
TTCGGGTCATGTGGCGGGTT+CGG 0.364904 4_1:+4363286 Msa0530320:CDS
CCATGCCTCTAAACATGATT+TGG 0.379481 4_1:+4363526 Msa0530320:CDS
TTGGCTGTTGGGCTAGGTGC+TGG 0.395448 4_1:+4363428 Msa0530320:CDS
GAAGAAGAATTCTTCGGCAT+TGG 0.403432 4_1:-4361058 None:intergenic
ATTGATTTGGCTGTTGGGCT+AGG 0.406845 4_1:+4363422 Msa0530320:CDS
GGGTGGTGTGTCTGCGTTTG+AGG 0.411025 4_1:-4361003 None:intergenic
TATATATTTGACTCTTTAGC+TGG 0.413885 4_1:+4363452 Msa0530320:CDS
TGGAATGGAATGAAGGCATT+AGG 0.429999 4_1:-4360924 None:intergenic
ACACTATTGATTTGGCTGTT+GGG 0.430277 4_1:+4363417 Msa0530320:CDS
AGAGGCGGCGCCGCCGTTCA+TGG 0.431943 4_1:-4360960 None:intergenic
CTGGTGTGGACTTTCCAGTT+GGG 0.441942 4_1:+4363240 Msa0530320:CDS
TAGAGTGATTGGTTTGATTG+GGG 0.454594 4_1:-4361024 None:intergenic
GAGGATGAAGAGGTGGGAGT+TGG 0.456112 4_1:+4363331 Msa0530320:CDS
TACACGTGGCAACACATCAT+TGG 0.460436 4_1:+4361137 Msa0530320:CDS
TTTACATGTCGTGGGATTTG+TGG 0.464311 4_1:+4362465 Msa0530320:CDS
CAAAGAGAAGAAGAATTCTT+CGG 0.480975 4_1:-4361064 None:intergenic
TCTGGTGTGGACTTTCCAGT+TGG 0.481025 4_1:+4363239 Msa0530320:CDS
TTGCAAGCTGTGAGGTTGCT+TGG 0.481402 4_1:+4363380 Msa0530320:CDS
AGAGTGATTGGTTTGATTGG+GGG 0.483457 4_1:-4361023 None:intergenic
TATAGAATTCGGAGAAAATG+AGG 0.485490 4_1:-4360891 None:intergenic
TTTGAGGACGGTGGCGTCAC+CGG 0.494293 4_1:-4360987 None:intergenic
GGGTTCGGTGATAGCTTCAT+TGG 0.496149 4_1:+4363301 Msa0530320:CDS
AATTTGAGACTGTACCCAAA+AGG 0.497856 4_1:+4363559 Msa0530320:three_prime_UTR
TGTTCTAGCTGCTCTTAATA+CGG 0.502013 4_1:+4361312 Msa0530320:CDS
TTATGGACATGGTTAATTGA+AGG 0.502135 4_1:+4362408 Msa0530320:CDS
GCGCCGCCGTTCATGGTGGT+TGG 0.505272 4_1:-4360953 None:intergenic
ATGGAATGAAGGCATTAGGA+GGG 0.507335 4_1:-4360920 None:intergenic
CACCTGGGGCAATGGTAGCT+GGG 0.507957 4_1:-4362496 None:intergenic
TTACATGTCGTGGGATTTGT+GGG 0.512699 4_1:+4362466 Msa0530320:CDS
CGACAAAATCATCTCCCATC+CGG 0.513619 4_1:-4363637 None:intergenic
GAATGAATCACCTGGGGCAA+TGG 0.515139 4_1:-4362504 None:intergenic
ATTTGAGACTGTACCCAAAA+GGG 0.515747 4_1:+4363560 Msa0530320:three_prime_UTR
ACATGAGGAGGATGAAGAGG+TGG 0.515861 4_1:+4363324 Msa0530320:CDS
AAAATGAGGTTGTAAATTGA+CGG 0.527236 4_1:-4360877 None:intergenic
CATGAGGAGGATGAAGAGGT+GGG 0.528033 4_1:+4363325 Msa0530320:CDS
TGAAGAGGTGGGAGTTGGCA+TGG 0.528699 4_1:+4363336 Msa0530320:CDS
GAAAACAAAACCCAAATCAA+AGG 0.528958 4_1:-4361242 None:intergenic
CAGCCAAATCAATAGTGTAA+TGG 0.530095 4_1:-4363412 None:intergenic
TTCATGGTGGTTGGAGAATG+TGG 0.541925 4_1:-4360944 None:intergenic
CCAAATCATGTTTAGAGGCA+TGG 0.542552 4_1:-4363526 None:intergenic
AAAACAAAACCCAAATCAAA+GGG 0.542870 4_1:-4361241 None:intergenic
TTGTTGTAGCTCTAAAGTGT+AGG 0.545152 4_1:+4363671 Msa0530320:three_prime_UTR
GGTGTGTCTGCGTTTGAGGA+CGG 0.546169 4_1:-4360999 None:intergenic
CGAGTTATATATTATGGACA+TGG 0.552668 4_1:+4362397 Msa0530320:CDS
AGACCACAAGCAAACAAACA+CGG 0.559564 4_1:-4361163 None:intergenic
AATGGAATGAAGGCATTAGG+AGG 0.560787 4_1:-4360921 None:intergenic
CACCCAGCTACCATTGCCCC+AGG 0.564037 4_1:+4362494 Msa0530320:CDS
AGAGCTGAGATTGTTGCTCT+AGG 0.568049 4_1:-4362435 None:intergenic
TCATGTTTGGATTCTAACAT+CGG 0.577627 4_1:-4363729 None:intergenic
CATCAACATCATAATAACTT+CGG 0.578990 4_1:+4360849 Msa0530320:five_prime_UTR
CATTCTCCAACCACCATGAA+CGG 0.579463 4_1:+4360947 Msa0530320:exon
GAGAATGTGGAATGGAATGA+AGG 0.579583 4_1:-4360931 None:intergenic
ATGCCGTGTTTGTTTGCTTG+TGG 0.580559 4_1:+4361160 Msa0530320:CDS
GTGTATGTGTGGATGCCGGA+TGG 0.581183 4_1:+4363622 Msa0530320:three_prime_UTR
TGGACATGAGGAGGATGAAG+AGG 0.583975 4_1:+4363321 Msa0530320:CDS
GTGAGGTTGCTTGGTACAAG+AGG 0.585032 4_1:+4363389 Msa0530320:CDS
TCAAAGGGTGGAGAAGAAGA+AGG 0.587930 4_1:-4361226 None:intergenic
ATGTGTGTATGTGTGGATGC+CGG 0.594876 4_1:+4363618 Msa0530320:three_prime_UTR
GGTGGTTGGAGAATGTGGAA+TGG 0.605709 4_1:-4360939 None:intergenic
TGTATGTGTGGATGCCGGAT+GGG 0.607384 4_1:+4363623 Msa0530320:three_prime_UTR
TTAGAGCTACAACAAAATCA+CGG 0.613938 4_1:-4363663 None:intergenic
GTAGGCAATGTGTGTATGTG+TGG 0.619686 4_1:+4363611 Msa0530320:three_prime_UTR
TGATAGCTTCATTGGACATG+AGG 0.619875 4_1:+4363309 Msa0530320:CDS
CTGGGGCAATGGTAGCTGGG+TGG 0.623185 4_1:-4362493 None:intergenic
GCTAGAACATTATTGAGCTG+AGG 0.646448 4_1:-4361298 None:intergenic
ACAAAACCCAAATCAAAGGG+TGG 0.648382 4_1:-4361238 None:intergenic
TAGCTTCATTGGACATGAGG+AGG 0.656474 4_1:+4363312 Msa0530320:CDS
GATGTTAGAATCCAAACATG+AGG 0.660469 4_1:+4363731 Msa0530320:three_prime_UTR
GTTAGAATCCAAACATGAGG+AGG 0.660606 4_1:+4363734 Msa0530320:three_prime_UTR
TGGAATGAAGGCATTAGGAG+GGG 0.675852 4_1:-4360919 None:intergenic
GGCGGCGCCGCCGTTCATGG+TGG 0.676201 4_1:-4360957 None:intergenic
TCTCCAACCACCATGAACGG+CGG 0.680353 4_1:+4360950 Msa0530320:exon
AATCTCAGATGTTGTACACG+TGG 0.689946 4_1:+4361123 Msa0530320:CDS
TGAATGTGTTGCAAGCTGTG+AGG 0.703330 4_1:+4363372 Msa0530320:CDS
GTGTCTGCGTTTGAGGACGG+TGG 0.706981 4_1:-4360996 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTGTTGATTTTTTTA+TGG + chr4_1:4361389-4361408 Msa0530320:intron 10.0%
!!! TTTTTTGTTGATTTTTTTAT+GGG + chr4_1:4361390-4361409 Msa0530320:intron 10.0%
!!! AGAATTGATTTTAATATTAT+TGG + chr4_1:4361754-4361773 Msa0530320:intron 10.0%
!!! AATTACTACTAATAATTTTA+GGG - chr4_1:4362679-4362698 None:intergenic 10.0%
!!! TTTTTCTTTGAGTTAAAAAT+TGG + chr4_1:4361683-4361702 Msa0530320:intron 15.0%
!!! TTGATTTTAATATTATTGGA+TGG + chr4_1:4361758-4361777 Msa0530320:intron 15.0%
!!! TATGATGAATTTATTTTTGT+TGG + chr4_1:4362083-4362102 Msa0530320:intron 15.0%
!! TTTAAAAAAATGAAACACTT+TGG - chr4_1:4362613-4362632 None:intergenic 15.0%
!!! ATTACTACTAATAATTTTAG+GGG - chr4_1:4362678-4362697 None:intergenic 15.0%
!!! GAATTACTACTAATAATTTT+AGG - chr4_1:4362680-4362699 None:intergenic 15.0%
!!! CTTAATAAATTTGAAATTTG+AGG - chr4_1:4363031-4363050 None:intergenic 15.0%
!! TGGATAAAAAACAGAAAAAT+TGG - chr4_1:4360743-4360762 None:intergenic 20.0%
!!! GAATAATTCTTTTCTTTTTG+TGG - chr4_1:4360763-4360782 None:intergenic 20.0%
!!! GTTTTTTTGCATTGTTTATT+TGG + chr4_1:4361502-4361521 Msa0530320:intron 20.0%
!!! TTTTGGTGTTTTTTTTTGTT+TGG + chr4_1:4361556-4361575 Msa0530320:intron 20.0%
!!! TTTTTTTTTGTTTGGCAATT+GGG + chr4_1:4361564-4361583 Msa0530320:intron 20.0%
!!! GATTTTTTGTTTGTTTCTAA+TGG + chr4_1:4361635-4361654 Msa0530320:intron 20.0%
!!! TATCTCTGAATTGATTTTTT+TGG + chr4_1:4361659-4361678 Msa0530320:intron 20.0%
!!! AAACTTTAAATTCTAGCTTT+AGG - chr4_1:4361825-4361844 None:intergenic 20.0%
!! AGATACATTCATATAAAAGT+GGG - chr4_1:4361936-4361955 None:intergenic 20.0%
!! TAGATACATTCATATAAAAG+TGG - chr4_1:4361937-4361956 None:intergenic 20.0%
!!! ATGAATTTATTTTTGTTGGT+AGG + chr4_1:4362087-4362106 Msa0530320:intron 20.0%
!!! TTTCTCTGAATTGATTTTTT+TGG + chr4_1:4362287-4362306 Msa0530320:intron 20.0%
!!! TGTTCCTTTATTTTATTGTA+TGG + chr4_1:4362637-4362656 Msa0530320:intron 20.0%
!!! GTTCCTTTATTTTATTGTAT+GGG + chr4_1:4362638-4362657 Msa0530320:intron 20.0%
!!! TCTTCATTTTTGAAGTATTT+GGG - chr4_1:4362856-4362875 None:intergenic 20.0%
!!! TTCTTCATTTTTGAAGTATT+TGG - chr4_1:4362857-4362876 None:intergenic 20.0%
!!! AAAAATGAAGAAGTATCAAT+TGG + chr4_1:4362865-4362884 Msa0530320:intron 20.0%
!! ATTTAAATTCCATTAACAAC+AGG + chr4_1:4363102-4363121 Msa0530320:intron 20.0%
!! TTACAATTACAAGACTAAAT+TGG + chr4_1:4363141-4363160 Msa0530320:intron 20.0%
!!! ATCATTTTTCCTGTTTTTTT+CGG + chr4_1:4363268-4363287 Msa0530320:CDS 20.0%
! CATCAACATCATAATAACTT+CGG + chr4_1:4360849-4360868 Msa0530320:five_prime_UTR 25.0%
! AAAATGAGGTTGTAAATTGA+CGG - chr4_1:4360880-4360899 None:intergenic 25.0%
!!! ATTTTTTTGGTTAGAGTGAT+TGG - chr4_1:4361038-4361057 None:intergenic 25.0%
! AAAACAAAACCCAAATCAAA+GGG - chr4_1:4361244-4361263 None:intergenic 25.0%
! ATCAATCAAACACATTACAT+CGG - chr4_1:4361423-4361442 None:intergenic 25.0%
! TGTTTGATTGATATGCAAAT+GGG + chr4_1:4361431-4361450 Msa0530320:intron 25.0%
!! GTCAAAATTTGTGTTTTCTT+AGG + chr4_1:4361526-4361545 Msa0530320:intron 25.0%
!! AATTTGTGTTTTCTTAGGTT+TGG + chr4_1:4361531-4361550 Msa0530320:intron 25.0%
!! TTTTCTTAGGTTTGGATTTT+TGG + chr4_1:4361539-4361558 Msa0530320:intron 25.0%
!!! GTTTTTTTTTGTTTGGCAAT+TGG + chr4_1:4361563-4361582 Msa0530320:intron 25.0%
! TGTTTGAATGACAAGAAAAT+GGG + chr4_1:4361597-4361616 Msa0530320:intron 25.0%
! GTTAAAAATTGGTGTATGTT+TGG + chr4_1:4361694-4361713 Msa0530320:intron 25.0%
!! GAGAATTTGAATTTGTTTCT+AGG + chr4_1:4361788-4361807 Msa0530320:intron 25.0%
!!! AATTTAAAGTTTTTGCCTCT+GGG + chr4_1:4361833-4361852 Msa0530320:intron 25.0%
!!! GAATTTTGCTGTTTTTTTTG+CGG + chr4_1:4362181-4362200 Msa0530320:intron 25.0%
!!! AATTTTGCTGTTTTTTTTGC+GGG + chr4_1:4362182-4362201 Msa0530320:intron 25.0%
!!! AATTTTGACACAAACCTATT+TGG - chr4_1:4362239-4362258 None:intergenic 25.0%
! TGCAAACGAGTTATATATTA+TGG + chr4_1:4362391-4362410 Msa0530320:CDS 25.0%
! TGAAAATGAAACACTTTGTT+TGG - chr4_1:4362559-4362578 None:intergenic 25.0%
! GTTCCCATACAATAAAATAA+AGG - chr4_1:4362644-4362663 None:intergenic 25.0%
!! ATTTGTCCTAATAACACTTT+TGG + chr4_1:4362760-4362779 Msa0530320:intron 25.0%
! GAAAGAACTAAATGTATGTT+TGG + chr4_1:4362802-4362821 Msa0530320:intron 25.0%
! CAATTAAATCCTGTTGTTAA+TGG - chr4_1:4363114-4363133 None:intergenic 25.0%
!!! TTTTGTGTTGATGTTAAACA+GGG + chr4_1:4363206-4363225 Msa0530320:intron 25.0%
!!! TCATTTTTCCTGTTTTTTTC+GGG + chr4_1:4363269-4363288 Msa0530320:CDS 25.0%
! TATATATTTGACTCTTTAGC+TGG + chr4_1:4363452-4363471 Msa0530320:CDS 25.0%
! ATAGCTAAAAATAGTGTCAA+TGG + chr4_1:4363503-4363522 Msa0530320:CDS 25.0%
! AAAAAGGATAATAGAGAAGT+AGG + chr4_1:4363593-4363612 Msa0530320:three_prime_UTR 25.0%
! TGTGGTTTGAATATATTTCT+AGG - chr4_1:4363934-4363953 None:intergenic 25.0%
!!! CTGTTCATTGTTTTGGAAAA+GGG - chr4_1:4360812-4360831 None:intergenic 30.0%
!!! ACTGTTCATTGTTTTGGAAA+AGG - chr4_1:4360813-4360832 None:intergenic 30.0%
! ACTACTACTGTTCATTGTTT+TGG - chr4_1:4360819-4360838 None:intergenic 30.0%
TATAGAATTCGGAGAAAATG+AGG - chr4_1:4360894-4360913 None:intergenic 30.0%
GAGGGGTTATTTATAGAATT+CGG - chr4_1:4360905-4360924 None:intergenic 30.0%
!! TTAGAGTGATTGGTTTGATT+GGG - chr4_1:4361028-4361047 None:intergenic 30.0%
CAAAGAGAAGAAGAATTCTT+CGG - chr4_1:4361067-4361086 None:intergenic 30.0%
GAAAACAAAACCCAAATCAA+AGG - chr4_1:4361245-4361264 None:intergenic 30.0%
AAGTGACAACAAAACACAAT+TGG - chr4_1:4361360-4361379 None:intergenic 30.0%
GTGTTTGATTGATATGCAAA+TGG + chr4_1:4361430-4361449 Msa0530320:intron 30.0%
GTGTTTGAATGACAAGAAAA+TGG + chr4_1:4361596-4361615 Msa0530320:intron 30.0%
!! GAATTTAAAGTTTTTGCCTC+TGG + chr4_1:4361832-4361851 Msa0530320:intron 30.0%
! TGGGTTGAACAATAAACTAA+AGG - chr4_1:4361917-4361936 None:intergenic 30.0%
!! TATGTGTTTTGTTGTGTTCA+AGG + chr4_1:4361955-4361974 Msa0530320:intron 30.0%
ACTTTAGTAACCTTACTCTT+AGG + chr4_1:4362122-4362141 Msa0530320:intron 30.0%
!! TGATGATTTGTTTGCATTGT+TGG + chr4_1:4362325-4362344 Msa0530320:intron 30.0%
! AATTTGTGTGTTTTGTGTGT+AGG + chr4_1:4362356-4362375 Msa0530320:intron 30.0%
CGAGTTATATATTATGGACA+TGG + chr4_1:4362397-4362416 Msa0530320:CDS 30.0%
TTATGGACATGGTTAATTGA+AGG + chr4_1:4362408-4362427 Msa0530320:CDS 30.0%
! TTTTCATGTTTACATGTCGT+GGG + chr4_1:4362457-4362476 Msa0530320:CDS 30.0%
ATGAAAAGAATGAATCACCT+GGG - chr4_1:4362514-4362533 None:intergenic 30.0%
AATGAAAAGAATGAATCACC+TGG - chr4_1:4362515-4362534 None:intergenic 30.0%
!! TAACCTCCAAAAGTGTTATT+AGG - chr4_1:4362769-4362788 None:intergenic 30.0%
!! TACATTTAGTTCTTTCAGCT+TGG - chr4_1:4362798-4362817 None:intergenic 30.0%
!! AAACATGCACTTAGTTCTTT+TGG - chr4_1:4362896-4362915 None:intergenic 30.0%
!!! GTTTTGGAAGCTTACAAATA+AGG + chr4_1:4362944-4362963 Msa0530320:intron 30.0%
TTGAGGCTAAAAGTCAAATT+TGG - chr4_1:4363014-4363033 None:intergenic 30.0%
!! GTTTTGTGTTGATGTTAAAC+AGG + chr4_1:4363205-4363224 Msa0530320:intron 30.0%
! TGATGTTAAACAGGGATTTT+TGG + chr4_1:4363214-4363233 Msa0530320:intron 30.0%
! GATGTTAAACAGGGATTTTT+GGG + chr4_1:4363215-4363234 Msa0530320:intron 30.0%
AAAAATGATACATTCCCAAC+TGG - chr4_1:4363257-4363276 None:intergenic 30.0%
TTAGAGCTACAACAAAATCA+CGG - chr4_1:4363666-4363685 None:intergenic 30.0%
!! TGTAGGTTTTGTCAAAATTG+CGG + chr4_1:4363688-4363707 Msa0530320:three_prime_UTR 30.0%
TCATGTTTGGATTCTAACAT+CGG - chr4_1:4363732-4363751 None:intergenic 30.0%
!!! CATTGTTTTGGAAAAGGGTA+AGG - chr4_1:4360807-4360826 None:intergenic 35.0%
!! TAGAGTGATTGGTTTGATTG+GGG - chr4_1:4361027-4361046 None:intergenic 35.0%
!! GTTAGAGTGATTGGTTTGAT+TGG - chr4_1:4361029-4361048 None:intergenic 35.0%
!!! TCTTCGGCATTGGATTTTTT+TGG - chr4_1:4361051-4361070 None:intergenic 35.0%
! TTGTGGTCTTTTGTTCTTCA+TGG + chr4_1:4361177-4361196 Msa0530320:CDS 35.0%
! TGTTCTAGCTGCTCTTAATA+CGG + chr4_1:4361312-4361331 Msa0530320:CDS 35.0%
CTTAATACGGTATCTCTCTT+TGG + chr4_1:4361325-4361344 Msa0530320:intron 35.0%
TTTGTTTGGCAATTGGGAAA+TGG + chr4_1:4361570-4361589 Msa0530320:intron 35.0%
! TTGGTGTATGTTTGGTTTCA+CGG + chr4_1:4361702-4361721 Msa0530320:intron 35.0%
!!! TTTGTGGGTGTTTTTGGTTT+TGG + chr4_1:4362159-4362178 Msa0530320:intron 35.0%
!!! CTGTTTTTTTTGCGGGAATT+GGG + chr4_1:4362189-4362208 Msa0530320:intron 35.0%
!!! TGTTTTTTTTGCGGGAATTG+GGG + chr4_1:4362190-4362209 Msa0530320:intron 35.0%
! GGTTTGGTTGAAATCCAAAT+AGG + chr4_1:4362222-4362241 Msa0530320:intron 35.0%
! GATTTGTTTGCATTGTTGGT+TGG + chr4_1:4362329-4362348 Msa0530320:intron 35.0%
! CTTTTCATGTTTACATGTCG+TGG + chr4_1:4362456-4362475 Msa0530320:CDS 35.0%
TGAAAAGAATGAATCACCTG+GGG - chr4_1:4362513-4362532 None:intergenic 35.0%
TATTAGGACAAATGCGATAG+CGG - chr4_1:4362753-4362772 None:intergenic 35.0%
! TGTCCTAATAACACTTTTGG+AGG + chr4_1:4362763-4362782 Msa0530320:intron 35.0%
!! TGCACTTAGTTCTTTTGGAT+TGG - chr4_1:4362891-4362910 None:intergenic 35.0%
! CTTTTTCTAACTTGAAGACG+AGG + chr4_1:4362973-4362992 Msa0530320:intron 35.0%
! AGGCCATTACACTATTGATT+TGG + chr4_1:4363409-4363428 Msa0530320:CDS 35.0%
CAGCCAAATCAATAGTGTAA+TGG - chr4_1:4363415-4363434 None:intergenic 35.0%
!! TACACTATTGATTTGGCTGT+TGG + chr4_1:4363416-4363435 Msa0530320:CDS 35.0%
!! ACACTATTGATTTGGCTGTT+GGG + chr4_1:4363417-4363436 Msa0530320:CDS 35.0%
TGCAACCAAATCATGTTTAG+AGG - chr4_1:4363534-4363553 None:intergenic 35.0%
! AATTTGAGACTGTACCCAAA+AGG + chr4_1:4363559-4363578 Msa0530320:three_prime_UTR 35.0%
! ATTTGAGACTGTACCCAAAA+GGG + chr4_1:4363560-4363579 Msa0530320:three_prime_UTR 35.0%
! TTTCTGTGTGTTTCCCTTTT+GGG - chr4_1:4363576-4363595 None:intergenic 35.0%
! TTTTCTGTGTGTTTCCCTTT+TGG - chr4_1:4363577-4363596 None:intergenic 35.0%
AAAGGGAAACACACAGAAAA+AGG + chr4_1:4363577-4363596 Msa0530320:three_prime_UTR 35.0%
!! TTGTTGTAGCTCTAAAGTGT+AGG + chr4_1:4363671-4363690 Msa0530320:three_prime_UTR 35.0%
GATGTTAGAATCCAAACATG+AGG + chr4_1:4363731-4363750 Msa0530320:three_prime_UTR 35.0%
! GCTTTGTCTGCATTTCATTT+TGG + chr4_1:4363894-4363913 Msa0530320:three_prime_UTR 35.0%
!! ATGGAATGAAGGCATTAGGA+GGG - chr4_1:4360923-4360942 None:intergenic 40.0%
!! AATGGAATGAAGGCATTAGG+AGG - chr4_1:4360924-4360943 None:intergenic 40.0%
!! TGGAATGGAATGAAGGCATT+AGG - chr4_1:4360927-4360946 None:intergenic 40.0%
GAGAATGTGGAATGGAATGA+AGG - chr4_1:4360934-4360953 None:intergenic 40.0%
!! AGAGTGATTGGTTTGATTGG+GGG - chr4_1:4361026-4361045 None:intergenic 40.0%
GAAGAAGAATTCTTCGGCAT+TGG - chr4_1:4361061-4361080 None:intergenic 40.0%
CTTCAGCTTCGTTCATGAAA+TGG + chr4_1:4361098-4361117 Msa0530320:CDS 40.0%
AATCTCAGATGTTGTACACG+TGG + chr4_1:4361123-4361142 Msa0530320:CDS 40.0%
AGACCACAAGCAAACAAACA+CGG - chr4_1:4361166-4361185 None:intergenic 40.0%
! TCTTTTGTTCTTCATGGCTG+TGG + chr4_1:4361183-4361202 Msa0530320:CDS 40.0%
TGTGGAATACACGCTCTTTA+TGG + chr4_1:4361201-4361220 Msa0530320:CDS 40.0%
TTCTTCTCCACCCTTTGATT+TGG + chr4_1:4361231-4361250 Msa0530320:CDS 40.0%
! TCTTCTCCACCCTTTGATTT+GGG + chr4_1:4361232-4361251 Msa0530320:CDS 40.0%
ACAAAACCCAAATCAAAGGG+TGG - chr4_1:4361241-4361260 None:intergenic 40.0%
GCTAGAACATTATTGAGCTG+AGG - chr4_1:4361301-4361320 None:intergenic 40.0%
CATGTTATCATCAATCCCAG+AGG - chr4_1:4361851-4361870 None:intergenic 40.0%
! AAACACCCACAAAGCATCAA+GGG - chr4_1:4362152-4362171 None:intergenic 40.0%
! AAAACACCCACAAAGCATCA+AGG - chr4_1:4362153-4362172 None:intergenic 40.0%
! TGATGCTTTGTGGGTGTTTT+TGG + chr4_1:4362153-4362172 Msa0530320:intron 40.0%
!!! GCTGTTTTTTTTGCGGGAAT+TGG + chr4_1:4362188-4362207 Msa0530320:intron 40.0%
TTTACATGTCGTGGGATTTG+TGG + chr4_1:4362465-4362484 Msa0530320:CDS 40.0%
TTACATGTCGTGGGATTTGT+GGG + chr4_1:4362466-4362485 Msa0530320:CDS 40.0%
! CTCAGAAGTGATGCTTGTTT+TGG + chr4_1:4362928-4362947 Msa0530320:intron 40.0%
TCTAACTTGAAGACGAGGTT+TGG + chr4_1:4362978-4362997 Msa0530320:intron 40.0%
GCTTCGCAAATTAGTGTCTT+AGG - chr4_1:4363065-4363084 None:intergenic 40.0%
!!! AAACAGGGATTTTTGGGTTC+TGG + chr4_1:4363221-4363240 Msa0530320:intron 40.0%
TGATAGCTTCATTGGACATG+AGG + chr4_1:4363309-4363328 Msa0530320:CDS 40.0%
CCAAATCATGTTTAGAGGCA+TGG - chr4_1:4363529-4363548 None:intergenic 40.0%
CCATGCCTCTAAACATGATT+TGG + chr4_1:4363526-4363545 Msa0530320:CDS 40.0%
GTTAGAATCCAAACATGAGG+AGG + chr4_1:4363734-4363753 Msa0530320:three_prime_UTR 40.0%
TTCAAACTCCTCCTCATGTT+TGG - chr4_1:4363745-4363764 None:intergenic 40.0%
!! TGGAATGAAGGCATTAGGAG+GGG - chr4_1:4360922-4360941 None:intergenic 45.0%
TTCATGGTGGTTGGAGAATG+TGG - chr4_1:4360947-4360966 None:intergenic 45.0%
CATTCTCCAACCACCATGAA+CGG + chr4_1:4360947-4360966 Msa0530320:exon 45.0%
TACACGTGGCAACACATCAT+TGG + chr4_1:4361137-4361156 Msa0530320:CDS 45.0%
ATGCCGTGTTTGTTTGCTTG+TGG + chr4_1:4361160-4361179 Msa0530320:CDS 45.0%
TCAAAGGGTGGAGAAGAAGA+AGG - chr4_1:4361229-4361248 None:intergenic 45.0%
! TTTTTGCGGGAATTGGGGAA+TGG + chr4_1:4362195-4362214 Msa0530320:intron 45.0%
! ATTGGGGAATGGAATGGGTT+TGG + chr4_1:4362206-4362225 Msa0530320:intron 45.0%
AGAGCTGAGATTGTTGCTCT+AGG - chr4_1:4362438-4362457 None:intergenic 45.0%
CCACATGACCCGAAAAAAAC+AGG - chr4_1:4363280-4363299 None:intergenic 45.0%
!!! CCTGTTTTTTTCGGGTCATG+TGG + chr4_1:4363277-4363296 Msa0530320:CDS 45.0%
!!! GTTTTTTTCGGGTCATGTGG+CGG + chr4_1:4363280-4363299 Msa0530320:CDS 45.0%
!!! TTTTTTTCGGGTCATGTGGC+GGG + chr4_1:4363281-4363300 Msa0530320:CDS 45.0%
TAGCTTCATTGGACATGAGG+AGG + chr4_1:4363312-4363331 Msa0530320:CDS 45.0%
TGAATGTGTTGCAAGCTGTG+AGG + chr4_1:4363372-4363391 Msa0530320:CDS 45.0%
!! ATTGATTTGGCTGTTGGGCT+AGG + chr4_1:4363422-4363441 Msa0530320:CDS 45.0%
GTAGGCAATGTGTGTATGTG+TGG + chr4_1:4363611-4363630 Msa0530320:three_prime_UTR 45.0%
ATGTGTGTATGTGTGGATGC+CGG + chr4_1:4363618-4363637 Msa0530320:three_prime_UTR 45.0%
CGACAAAATCATCTCCCATC+CGG - chr4_1:4363640-4363659 None:intergenic 45.0%
GGTGGTTGGAGAATGTGGAA+TGG - chr4_1:4360942-4360961 None:intergenic 50.0%
!! GTGATTGGTTTGATTGGGGG+TGG - chr4_1:4361023-4361042 None:intergenic 50.0%
CAAGGGCTGACCTAAGAGTA+AGG - chr4_1:4362135-4362154 None:intergenic 50.0%
GTCAGCCCTTGATGCTTTGT+GGG + chr4_1:4362144-4362163 Msa0530320:intron 50.0%
CGGGAATTGGGGAATGGAAT+GGG + chr4_1:4362201-4362220 Msa0530320:intron 50.0%
GAATGAATCACCTGGGGCAA+TGG - chr4_1:4362507-4362526 None:intergenic 50.0%
!!! GGGATTTTTGGGTTCTGGTG+TGG + chr4_1:4363226-4363245 Msa0530320:intron 50.0%
TCTGGTGTGGACTTTCCAGT+TGG + chr4_1:4363239-4363258 Msa0530320:CDS 50.0%
CTGGTGTGGACTTTCCAGTT+GGG + chr4_1:4363240-4363259 Msa0530320:CDS 50.0%
!! GGGTTCGGTGATAGCTTCAT+TGG + chr4_1:4363301-4363320 Msa0530320:CDS 50.0%
TGGACATGAGGAGGATGAAG+AGG + chr4_1:4363321-4363340 Msa0530320:CDS 50.0%
ACATGAGGAGGATGAAGAGG+TGG + chr4_1:4363324-4363343 Msa0530320:CDS 50.0%
CATGAGGAGGATGAAGAGGT+GGG + chr4_1:4363325-4363344 Msa0530320:CDS 50.0%
TTGCAAGCTGTGAGGTTGCT+TGG + chr4_1:4363380-4363399 Msa0530320:CDS 50.0%
GTGAGGTTGCTTGGTACAAG+AGG + chr4_1:4363389-4363408 Msa0530320:CDS 50.0%
TGTATGTGTGGATGCCGGAT+GGG + chr4_1:4363623-4363642 Msa0530320:three_prime_UTR 50.0%
TCTCCAACCACCATGAACGG+CGG + chr4_1:4360950-4360969 Msa0530320:exon 55.0%
GGTGTGTCTGCGTTTGAGGA+CGG - chr4_1:4361002-4361021 None:intergenic 55.0%
GGTCAGCCCTTGATGCTTTG+TGG + chr4_1:4362143-4362162 Msa0530320:intron 55.0%
GCGGGAATTGGGGAATGGAA+TGG + chr4_1:4362200-4362219 Msa0530320:intron 55.0%
GAGGATGAAGAGGTGGGAGT+TGG + chr4_1:4363331-4363350 Msa0530320:CDS 55.0%
TGAAGAGGTGGGAGTTGGCA+TGG + chr4_1:4363336-4363355 Msa0530320:CDS 55.0%
GTGTATGTGTGGATGCCGGA+TGG + chr4_1:4363622-4363641 Msa0530320:three_prime_UTR 55.0%
GGCGTCACCGGAAAAAGAGG+CGG - chr4_1:4360978-4360997 None:intergenic 60.0%
GGTGGCGTCACCGGAAAAAG+AGG - chr4_1:4360981-4361000 None:intergenic 60.0%
TTTGAGGACGGTGGCGTCAC+CGG - chr4_1:4360990-4361009 None:intergenic 60.0%
GTGTCTGCGTTTGAGGACGG+TGG - chr4_1:4360999-4361018 None:intergenic 60.0%
GGGTGGTGTGTCTGCGTTTG+AGG - chr4_1:4361006-4361025 None:intergenic 60.0%
CACCTGGGGCAATGGTAGCT+GGG - chr4_1:4362499-4362518 None:intergenic 60.0%
TCACCTGGGGCAATGGTAGC+TGG - chr4_1:4362500-4362519 None:intergenic 60.0%
!! TTCGGGTCATGTGGCGGGTT+CGG + chr4_1:4363286-4363305 Msa0530320:CDS 60.0%
! TTGGCTGTTGGGCTAGGTGC+TGG + chr4_1:4363428-4363447 Msa0530320:CDS 60.0%
CTGGGGCAATGGTAGCTGGG+TGG - chr4_1:4362496-4362515 None:intergenic 65.0%
CACCCAGCTACCATTGCCCC+AGG + chr4_1:4362494-4362513 Msa0530320:CDS 65.0%
GCGCCGCCGTTCATGGTGGT+TGG - chr4_1:4360956-4360975 None:intergenic 70.0%
! GGCGGCGCCGCCTCTTTTTC+CGG + chr4_1:4360968-4360987 Msa0530320:CDS 70.0%
AGAGGCGGCGCCGCCGTTCA+TGG - chr4_1:4360963-4360982 None:intergenic 75.0%
! GGCGGCGCCGCCGTTCATGG+TGG - chr4_1:4360960-4360979 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 4360740 4363966 4360740 ID=Msa0530320;Name=Msa0530320
chr4_1 mRNA 4360740 4363966 4360740 ID=Msa0530320-mRNA-1;Parent=Msa0530320;Name=Msa0530320-mRNA-1;_AED=0.30;_eAED=0.30;_QI=222|1|1|1|1|1|3|410|279
chr4_1 exon 4360740 4361333 4360740 ID=Msa0530320-mRNA-1:exon:1295;Parent=Msa0530320-mRNA-1
chr4_1 exon 4362378 4362515 4362378 ID=Msa0530320-mRNA-1:exon:1296;Parent=Msa0530320-mRNA-1
chr4_1 exon 4363227 4363966 4363227 ID=Msa0530320-mRNA-1:exon:1297;Parent=Msa0530320-mRNA-1
chr4_1 five_prime_UTR 4360740 4360961 4360740 ID=Msa0530320-mRNA-1:five_prime_utr;Parent=Msa0530320-mRNA-1
chr4_1 CDS 4360962 4361333 4360962 ID=Msa0530320-mRNA-1:cds;Parent=Msa0530320-mRNA-1
chr4_1 CDS 4362378 4362515 4362378 ID=Msa0530320-mRNA-1:cds;Parent=Msa0530320-mRNA-1
chr4_1 CDS 4363227 4363556 4363227 ID=Msa0530320-mRNA-1:cds;Parent=Msa0530320-mRNA-1
chr4_1 three_prime_UTR 4363557 4363966 4363557 ID=Msa0530320-mRNA-1:three_prime_utr;Parent=Msa0530320-mRNA-1
Gene Sequence

>Msa0530320

ATGAACGGCGGCGCCGCCTCTTTTTCCGGTGACGCCACCGTCCTCAAACGCAGACACACCACCCCCAATCAAACCAATCACTCTAACCAAAAAAATCCAATGCCGAAGAATTCTTCTTCTCTTTGTTCTCTGTATTCTTCAGCTTCGTTCATGAAATGGACAATCTCAGATGTTGTACACGTGGCAACACATCATTGGATGCCGTGTTTGTTTGCTTGTGGTCTTTTGTTCTTCATGGCTGTGGAATACACGCTCTTTATGGTTCCTTCTTCTTCTCCACCCTTTGATTTGGGTTTTGTTTTCACGCGTGATCTTCATCGTGTTCTTGAATCTTCTCCTCAGCTCAATAATGTTCTAGCTGCTCTTAATACGGTGTTTGTGTTAATGCAAACGAGTTATATATTATGGACATGGTTAATTGAAGGAAGACCTAGAGCAACAATCTCAGCTCTTTTCATGTTTACATGTCGTGGGATTTGTGGGTATTCCACCCAGCTACCATTGCCCCAGGGATTTTTGGGTTCTGGTGTGGACTTTCCAGTTGGGAATGTATCATTTTTCCTGTTTTTTTCGGGTCATGTGGCGGGTTCGGTGATAGCTTCATTGGACATGAGGAGGATGAAGAGGTGGGAGTTGGCATGGACTTTTGATTTGTTGAATGTGTTGCAAGCTGTGAGGTTGCTTGGTACAAGAGGCCATTACACTATTGATTTGGCTGTTGGGCTAGGTGCTGGCTATATATTTGACTCTTTAGCTGGTAAGTATCTTGAAGATAGCAAGTTGAAAATAGCTAAAAATAGTGTCAATGGCCATGCCTCTAAACATGATTTGGTTGCATGA
Protein sequence

>Msa0530320

MNGGAASFSGDATVLKRRHTTPNQTNHSNQKNPMPKNSSSLCSLYSSASFMKWTISDVVHVATHHWMPCLFACGLLFFMAVEYTLFMVPSSSPPFDLGFVFTRDLHRVLESSPQLNNVLAALNTVFVLMQTSYILWTWLIEGRPRATISALFMFTCRGICGYSTQLPLPQGFLGSGVDFPVGNVSFFLFFSGHVAGSVIASLDMRRMKRWELAWTFDLLNVLQAVRLLGTRGHYTIDLAVGLGAGYIFDSLAGKYLEDSKLKIAKNSVNGHASKHDLVA*