AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0535370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0535370 MtrunA17_Chr4g0009431 98.933 375 4 0 1 375 102 476 0.0 778
Msa0535370 MtrunA17_Chr5g0435631 88.770 374 42 0 2 375 102 475 0.0 718
Msa0535370 MtrunA17_Chr3g0112121 89.254 335 36 0 2 336 102 436 0.0 639
Msa0535370 MtrunA17_Chr2g0334101 53.767 292 128 6 33 318 123 413 1.02e-109 327
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0535370 AT2G39730.1 84.267 375 59 0 1 375 100 474 0.0 682
Msa0535370 AT2G39730.2 83.478 345 57 0 1 345 100 444 0.0 620
Msa0535370 AT2G39730.3 84.661 339 52 0 1 339 100 438 0.0 618
Msa0535370 AT1G73110.2 51.724 319 145 7 6 318 15 330 2.34e-111 329
Msa0535370 AT1G73110.1 52.564 312 139 7 13 318 119 427 7.46e-110 328

Find 128 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTATATTGCACCTGCATTTA+TGG 0.088800 4_1:-11618495 Msa0535370:CDS
TATTAAGATTCCTCTCATTT+TGG 0.116167 4_1:-11618426 Msa0535370:CDS
CATGCGTCCATCACGAATTA+AGG 0.178930 4_1:+11617843 None:intergenic
GAAGGGTCAGCGCAACCTTC+TGG 0.200974 4_1:+11616932 None:intergenic
ATGAGAGGAATCTTAATATT+TGG 0.205578 4_1:+11618431 None:intergenic
TCTTAACATTCTCTTGTTCT+TGG 0.240524 4_1:+11617208 None:intergenic
TCTCCGGCACTCATCATTAT+AGG 0.240969 4_1:+11618165 None:intergenic
TGCATTAATCTCACCACTTT+AGG 0.260377 4_1:-11616749 Msa0535370:three_prime_UTR
TGAGGTATTTGTCAGCTAAT+TGG 0.262434 4_1:+11617181 None:intergenic
TGATATGTTCAGGTACAATA+TGG 0.328301 4_1:-11618537 Msa0535370:intron
CTTTGCCTAATCAACTTTGC+AGG 0.332302 4_1:+11618117 None:intergenic
AATGCACCTTACATAAAATA+TGG 0.337542 4_1:+11616766 None:intergenic
TCGCGGAACTCGCTTTCTTA+AGG 0.339271 4_1:+11619310 None:intergenic
TCATTGCCAGTGACGATGAT+AGG 0.347209 4_1:+11617886 None:intergenic
TCTTTGCCAAGATGGGAATA+AGG 0.350452 4_1:-11618355 Msa0535370:intron
CTTAAGTGAGTACATAAATA+TGG 0.350516 4_1:-11616842 Msa0535370:three_prime_UTR
GCAACTTCCATCGTCACTTC+TGG 0.351528 4_1:+11616886 None:intergenic
AATGATGAGTGCCGGAGAAT+TGG 0.354739 4_1:-11618160 Msa0535370:CDS
ATCAAGACTGGAAACTTCTA+TGG 0.360535 4_1:-11617123 Msa0535370:intron
TCCAGCCCATCTGTCCTTTG+AGG 0.369839 4_1:+11619215 None:intergenic
CTTGACAAGCTACTAGAGTA+TGG 0.372362 4_1:-11617243 Msa0535370:CDS
GGGCGCCTACTCGTGATGAT+AGG 0.379460 4_1:-11617807 Msa0535370:CDS
GTCCACCTTTCCCTTTCCTC+TGG 0.384827 4_1:+11619155 None:intergenic
CTCTTCTTTGTTGTACATAC+CGG 0.388448 4_1:+11617921 None:intergenic
AATTACCAGACAAACTCTTC+TGG 0.388595 4_1:+11619280 None:intergenic
AGCTTGTCAAGAGTCATCTT+TGG 0.390025 4_1:+11617255 None:intergenic
CTCCGGCACTCATCATTATA+GGG 0.391535 4_1:+11618166 None:intergenic
AAGGGTCAGCGCAACCTTCT+GGG 0.410738 4_1:+11616933 None:intergenic
CCTACCAATGTTCAACTTCC+CGG 0.413313 4_1:-11617940 Msa0535370:CDS
AAGATTGTCGACACATTCCC+TGG 0.416886 4_1:-11617727 Msa0535370:CDS
TGAGGGTTGCATTTACCATC+TGG 0.421559 4_1:+11617980 None:intergenic
ATTTCCACTTTCCAATTCTC+CGG 0.426624 4_1:+11618149 None:intergenic
GAAGTCAGGAATTGGATATC+TGG 0.433568 4_1:-11617345 Msa0535370:CDS
GAGAGCCTGCAAAGTTGATT+AGG 0.436687 4_1:-11618122 Msa0535370:CDS
TACCTCAGTGAGGCAGCTCT+TGG 0.438518 4_1:-11617165 Msa0535370:CDS
ATGACGATGAAGTCAGGAAT+TGG 0.439434 4_1:-11617353 Msa0535370:CDS
TCCAGCCAGAAGAGTTTGTC+TGG 0.441643 4_1:-11619285 Msa0535370:five_prime_UTR
AATATGGACAACACAATAGA+TGG 0.441782 4_1:-11618521 Msa0535370:CDS
ATCTTTGGTTGCTCGAAAGT+TGG 0.445047 4_1:+11617270 None:intergenic
AAGAACAAGAGAATGTTAAG+AGG 0.447124 4_1:-11617206 Msa0535370:CDS
ATCTTGACAATGTCATCCTT+AGG 0.455344 4_1:+11617745 None:intergenic
ACAAAATATACAACCTAAAG+TGG 0.462806 4_1:+11616736 None:intergenic
AATAATGATGCTATCAAGAC+TGG 0.465950 4_1:-11617135 Msa0535370:CDS
CAGGATATCACCAGAGGAAA+GGG 0.467193 4_1:-11619165 Msa0535370:five_prime_UTR
CAACCTTCTGGGACAGGTAA+AGG 0.470524 4_1:+11616944 None:intergenic
ACATTCCCTGGACAGTCAAT+TGG 0.470564 4_1:-11617715 Msa0535370:intron
TATGTGCAGTTGAAATTGAA+TGG 0.472948 4_1:-11619381 None:intergenic
GGCTTACCAATTGACTGTCC+AGG 0.479453 4_1:+11617709 None:intergenic
TTTGGTTGCTCGAAAGTTGG+AGG 0.479859 4_1:+11617273 None:intergenic
ACCTCAAAGGACAGATGGGC+TGG 0.482103 4_1:-11619216 Msa0535370:five_prime_UTR
TTGGGCATTTGGGGAGGCAA+AGG 0.485012 4_1:-11618407 Msa0535370:CDS
TTCCTCTGGTGATATCCTGT+TGG 0.486672 4_1:+11619169 None:intergenic
GCGTCCGACATTATCAGAAA+AGG 0.491800 4_1:-11618084 Msa0535370:CDS
TTCATCAATGATCTTGATGC+AGG 0.493471 4_1:-11618045 Msa0535370:CDS
GAGTGTATGACGATGAAGTC+AGG 0.497686 4_1:-11617359 Msa0535370:CDS
CCGGGAAGTTGAACATTGGT+AGG 0.497705 4_1:+11617940 None:intergenic
CAGTGTCTTTCAAGCTCCAA+TGG 0.501662 4_1:-11619133 Msa0535370:exon
TGACACCTCAGATGACCAAC+AGG 0.501897 4_1:-11619184 Msa0535370:five_prime_UTR
TTTCAAGCTCCAATGGATGC+TGG 0.504462 4_1:-11619126 Msa0535370:exon
ACCAACTTACTGGCGAAGAC+CGG 0.505874 4_1:+11619062 None:intergenic
TTGGTCATCTGAGGTGTCAT+AGG 0.510724 4_1:+11619188 None:intergenic
GGCGCCTACTCGTGATGATA+GGG 0.511511 4_1:-11617806 Msa0535370:CDS
TCCTATGATTATATCAGTAC+CGG 0.513038 4_1:-11619081 Msa0535370:CDS
ATGCGTCCATCACGAATTAA+GGG 0.513590 4_1:+11617844 None:intergenic
GGTATTGGCGTGGAGAGTAT+TGG 0.514744 4_1:-11617324 Msa0535370:CDS
CAGGACAGATGGGGTCCCTA+AGG 0.516866 4_1:-11617761 Msa0535370:CDS
TCAGCGCAACCTTCTGGGAC+AGG 0.517907 4_1:+11616938 None:intergenic
TCTTGACAATGTCATCCTTA+GGG 0.521614 4_1:+11617746 None:intergenic
AGGAATTGGATATCTGGTAT+TGG 0.523533 4_1:-11617339 Msa0535370:CDS
AGCAGACCTCAAAGGACAGA+TGG 0.523593 4_1:-11619221 Msa0535370:five_prime_UTR
ACGAATTAAGGGAGCATACA+AGG 0.524450 4_1:+11617855 None:intergenic
ACCAGACAAACTCTTCTGGC+TGG 0.530162 4_1:+11619284 None:intergenic
CGTGTCCCTATCATCGTCAC+TGG 0.534166 4_1:-11617892 Msa0535370:CDS
AAGACTTGTGAATTCAAAAG+AGG 0.535398 4_1:-11617298 Msa0535370:CDS
TGATAATGTCGGACGCCTCA+CGG 0.537380 4_1:+11618091 None:intergenic
AATGATCTTGATGCAGGAGC+TGG 0.538362 4_1:-11618039 Msa0535370:CDS
CATTGCCAGTGACGATGATA+GGG 0.540834 4_1:+11617887 None:intergenic
AATCAACTTTGCAGGCTCTC+CGG 0.542852 4_1:+11618125 None:intergenic
TGAGCTTGTCTTTGCCAAGA+TGG 0.545502 4_1:-11618363 Msa0535370:CDS
GATGCAGGAGCTGGTAGAAT+GGG 0.546512 4_1:-11618030 Msa0535370:CDS
TATGCTCCCTTAATTCGTGA+TGG 0.548955 4_1:-11617850 Msa0535370:CDS
ACCGGTACTGATATAATCAT+AGG 0.550230 4_1:+11619080 None:intergenic
GCTTACCAATTGACTGTCCA+GGG 0.556345 4_1:+11617710 None:intergenic
GAATTGGAAAGTGGAAATGC+CGG 0.558234 4_1:-11618144 Msa0535370:CDS
TAGTGAGTTCCAGCATCCAT+TGG 0.558625 4_1:+11619117 None:intergenic
ACAGGATATCACCAGAGGAA+AGG 0.560580 4_1:-11619166 Msa0535370:five_prime_UTR
CCATCGTCACTTCTGGCTGT+CGG 0.561082 4_1:+11616893 None:intergenic
ATTTGGGGAGGCAAAGGTCA+AGG 0.561672 4_1:-11618401 Msa0535370:CDS
ACAAGCTTGTCCATAAATGC+AGG 0.573184 4_1:+11618485 None:intergenic
TGATGCAGGAGCTGGTAGAA+TGG 0.573216 4_1:-11618031 Msa0535370:CDS
ACTCTTCTGGCTGGAAATCG+CGG 0.577441 4_1:+11619293 None:intergenic
GGTTGTCAGCAATGTTCATG+AGG 0.579374 4_1:+11617962 None:intergenic
TCTTCTTTGTTGTACATACC+GGG 0.579969 4_1:+11617922 None:intergenic
GTTCCTTTACCTGTCCCAGA+AGG 0.583970 4_1:-11616947 Msa0535370:CDS
GTCATACACTCTAGCCCTCA+AGG 0.585052 4_1:+11617371 None:intergenic
AGCCCTATAATGATGAGTGC+CGG 0.586108 4_1:-11618168 Msa0535370:CDS
GACACTTATAGAAAGCAAGT+TGG 0.586229 4_1:-11616679 Msa0535370:three_prime_UTR
CGGGAAGTTGAACATTGGTA+GGG 0.586332 4_1:+11617941 None:intergenic
CTTAATTCGTGATGGACGCA+TGG 0.586707 4_1:-11617842 Msa0535370:CDS
CCCCAAATGCCCAAAATGAG+AGG 0.592582 4_1:+11618416 None:intergenic
AGATGATTGTGCAAAATGAA+AGG 0.592889 4_1:+11616642 None:intergenic
GAGCTTGTCTTTGCCAAGAT+GGG 0.595810 4_1:-11618362 Msa0535370:CDS
GCAGACCTCAAAGGACAGAT+GGG 0.599753 4_1:-11619220 Msa0535370:five_prime_UTR
GATATCCTGTTGGTCATCTG+AGG 0.602459 4_1:+11619179 None:intergenic
AGAACAAGAGAATGTTAAGA+GGG 0.603801 4_1:-11617205 Msa0535370:CDS
AAATTGAATGGTTCTAGCAA+TGG 0.604388 4_1:-11619369 Msa0535370:five_prime_UTR
GTTGTCAGCAATGTTCATGA+GGG 0.605287 4_1:+11617963 None:intergenic
AGACTTGTGAATTCAAAAGA+GGG 0.610232 4_1:-11617297 Msa0535370:CDS
GATAGGGTTGGTGTTTGTCA+AGG 0.612009 4_1:-11617790 Msa0535370:CDS
CACCAGAGGAAAGGGAAAGG+TGG 0.620977 4_1:-11619157 Msa0535370:five_prime_UTR
TTGGATATCTGGTATTGGCG+TGG 0.621027 4_1:-11617334 Msa0535370:CDS
CAGTGACGATGATAGGGACA+CGG 0.622622 4_1:+11617893 None:intergenic
AGTGCCGGAGAATTGGAAAG+TGG 0.623122 4_1:-11618153 Msa0535370:CDS
CTGCAAAGTTGATTAGGCAA+AGG 0.623429 4_1:-11618116 Msa0535370:CDS
CCTACTCGTGATGATAGGGT+TGG 0.628308 4_1:-11617802 Msa0535370:CDS
TGATTGTGCAAAATGAAAGG+AGG 0.628397 4_1:+11616645 None:intergenic
CCGACAGCCAGAAGTGACGA+TGG 0.630990 4_1:-11616893 Msa0535370:CDS
TGAGGAGAAGCAGACCTCAA+AGG 0.633145 4_1:-11619229 Msa0535370:five_prime_UTR
CATACCGGGAAGTTGAACAT+TGG 0.657559 4_1:+11617936 None:intergenic
AGTGACGATGATAGGGACAC+GGG 0.662449 4_1:+11617894 None:intergenic
TATCACCAGAGGAAAGGGAA+AGG 0.664919 4_1:-11619160 Msa0535370:five_prime_UTR
ATACACAGTCAATAACCAGA+TGG 0.667649 4_1:-11617995 Msa0535370:CDS
CCAACCCTATCATCACGAGT+AGG 0.671567 4_1:+11617802 None:intergenic
GCAGGAGCTGGTAGAATGGG+TGG 0.672116 4_1:-11618027 Msa0535370:CDS
GACCAACAGGATATCACCAG+AGG 0.697074 4_1:-11619171 Msa0535370:five_prime_UTR
CTCCAAGAGCTGCCTCACTG+AGG 0.729949 4_1:+11617163 None:intergenic
GATTAGGCAAAGGTACCGTG+AGG 0.742836 4_1:-11618106 Msa0535370:CDS
AGCTGACAAATACCTCAGTG+AGG 0.750394 4_1:-11617175 Msa0535370:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAAATTTTGAATTATTTTG+AGG - chr4_1:11617340-11617359 Msa0535370:CDS 10.0%
!!! ATGCTAATTTACTTATTTTT+AGG - chr4_1:11617066-11617085 Msa0535370:intron 15.0%
!! TAATAGATATTTGTATCAAT+TGG + chr4_1:11617156-11617175 None:intergenic 15.0%
!! CATGGTTAATAATAAATATA+AGG + chr4_1:11618564-11618583 None:intergenic 15.0%
!!! TGTATATTTTGTTTTATTGA+TGG - chr4_1:11619302-11619321 Msa0535370:five_prime_UTR 15.0%
!! AATCTTTGTTAACTGAATTA+AGG + chr4_1:11616995-11617014 None:intergenic 20.0%
!!! TACATAAATTGATAACACTT+CGG + chr4_1:11617038-11617057 None:intergenic 20.0%
!!! TTTACTTATTTTTAGGTACA+TGG - chr4_1:11617073-11617092 Msa0535370:intron 20.0%
!!! TTCCTGTATTATTTGATTTA+TGG - chr4_1:11617316-11617335 Msa0535370:CDS 20.0%
!!! ATTTTGAATTATTTTGAGGT+TGG - chr4_1:11617344-11617363 Msa0535370:CDS 20.0%
!! TGATATTCTAGGTTATAATA+TGG - chr4_1:11617371-11617390 Msa0535370:CDS 20.0%
!! ATAAATATAAGGATCTTGTT+TGG + chr4_1:11618553-11618572 None:intergenic 20.0%
!!! CATTTCTTTGTAGATTTTTT+TGG - chr4_1:11618631-11618650 Msa0535370:intron 20.0%
!! GATAATAACAGAAAAATCAA+AGG + chr4_1:11618935-11618954 None:intergenic 20.0%
!! AAACAATAAGATAACATTCT+TGG + chr4_1:11619035-11619054 None:intergenic 20.0%
!! AAGAATGTTATCTTATTGTT+TGG - chr4_1:11619034-11619053 Msa0535370:intron 20.0%
!!! TTGACAAATTCAGCATTTTT+TGG - chr4_1:11616688-11616707 Msa0535370:three_prime_UTR 25.0%
! GTGTTATCAATTTATGTACT+TGG - chr4_1:11617040-11617059 Msa0535370:intron 25.0%
! TATCAGTATCTTACGATATA+TGG - chr4_1:11617097-11617116 Msa0535370:intron 25.0%
! AGCCATAAATCAAATAATAC+AGG + chr4_1:11617321-11617340 None:intergenic 25.0%
! ATGAGAGGAATCTTAATATT+TGG + chr4_1:11617599-11617618 None:intergenic 25.0%
!! TATTAAGATTCCTCTCATTT+TGG - chr4_1:11617601-11617620 Msa0535370:intron 25.0%
!! ATTAAGATTCCTCTCATTTT+GGG - chr4_1:11617602-11617621 Msa0535370:intron 25.0%
! AAGGTAAAAAGAAATTTGAC+AGG - chr4_1:11617691-11617710 Msa0535370:intron 25.0%
! TTGAAACTTAGATTGATGTA+TGG + chr4_1:11618342-11618361 None:intergenic 25.0%
!!! AGTCAAAATTTCTAGTTACT+AGG - chr4_1:11618361-11618380 Msa0535370:CDS 25.0%
!!! GTCAAAATTTCTAGTTACTA+GGG - chr4_1:11618362-11618381 Msa0535370:CDS 25.0%
! CTTAAGTGAGTACATAAATA+TGG - chr4_1:11619185-11619204 Msa0535370:five_prime_UTR 25.0%
!! CATTCACCATATTTTATGTA+AGG - chr4_1:11619255-11619274 Msa0535370:five_prime_UTR 25.0%
! AATGCACCTTACATAAAATA+TGG + chr4_1:11619264-11619283 None:intergenic 25.0%
! ACAAAATATACAACCTAAAG+TGG + chr4_1:11619294-11619313 None:intergenic 25.0%
!! AAATTGAATGGTTCTAGCAA+TGG - chr4_1:11616658-11616677 Msa0535370:three_prime_UTR 30.0%
! GAGTTTGTCTGGTAATTTTA+AGG - chr4_1:11616753-11616772 Msa0535370:three_prime_UTR 30.0%
!!! GTCTGGTAATTTTAAGGTTT+CGG - chr4_1:11616759-11616778 Msa0535370:three_prime_UTR 30.0%
GGCAAAAATTGACTATAATG+AGG - chr4_1:11616780-11616799 Msa0535370:three_prime_UTR 30.0%
TCCTATGATTATATCAGTAC+CGG - chr4_1:11616946-11616965 Msa0535370:CDS 30.0%
! TTAACAAAGATTTGAAGCTC+AGG - chr4_1:11617003-11617022 Msa0535370:intron 30.0%
!! TGACATTTTTAAGCAAGAAC+AGG + chr4_1:11617405-11617424 None:intergenic 30.0%
TTTGATTCACGATTTGAATC+TGG - chr4_1:11617464-11617483 Msa0535370:intron 30.0%
AATCTGGATTTGATATGTTC+AGG - chr4_1:11617480-11617499 Msa0535370:intron 30.0%
TGATATGTTCAGGTACAATA+TGG - chr4_1:11617490-11617509 Msa0535370:intron 30.0%
AATATGGACAACACAATAGA+TGG - chr4_1:11617506-11617525 Msa0535370:intron 30.0%
! TTTTTACCTTATTCCCATCT+TGG + chr4_1:11617681-11617700 None:intergenic 30.0%
AACTCTTTGATCTACTTCTT+TGG - chr4_1:11617831-11617850 Msa0535370:CDS 30.0%
!! CTTTCCTTTTCTGATAATGT+CGG + chr4_1:11617950-11617969 None:intergenic 30.0%
ACGCATGGAAAAATTCTATT+GGG - chr4_1:11618200-11618219 Msa0535370:intron 30.0%
AATGATCTGAAAACAGCTTA+TGG + chr4_1:11618460-11618479 None:intergenic 30.0%
AAGACTTGTGAATTCAAAAG+AGG - chr4_1:11618729-11618748 Msa0535370:intron 30.0%
AGACTTGTGAATTCAAAAGA+GGG - chr4_1:11618730-11618749 Msa0535370:intron 30.0%
TCTTAACATTCTCTTGTTCT+TGG + chr4_1:11618822-11618841 None:intergenic 30.0%
AAGAACAAGAGAATGTTAAG+AGG - chr4_1:11618821-11618840 Msa0535370:intron 30.0%
AGAACAAGAGAATGTTAAGA+GGG - chr4_1:11618822-11618841 Msa0535370:intron 30.0%
AATAATGATGCTATCAAGAC+TGG - chr4_1:11618892-11618911 Msa0535370:intron 30.0%
! TATCACCTAAAAGTTTGCTT+TGG - chr4_1:11618964-11618983 Msa0535370:intron 30.0%
! TTGATCCAAAGCAAACTTTT+AGG + chr4_1:11618972-11618991 None:intergenic 30.0%
TTATCTTATTGTTTGGATGC+AGG - chr4_1:11619041-11619060 Msa0535370:intron 30.0%
!!! GTTTTATTGATGGTGTCTTA+TGG - chr4_1:11619312-11619331 Msa0535370:five_prime_UTR 30.0%
AATTACCAGACAAACTCTTC+TGG + chr4_1:11616750-11616769 None:intergenic 35.0%
!! ACCGGTACTGATATAATCAT+AGG + chr4_1:11616950-11616969 None:intergenic 35.0%
!! AGGTTGGTAGATGATATTCT+AGG - chr4_1:11617360-11617379 Msa0535370:CDS 35.0%
GTATATTGCACCTGCATTTA+TGG - chr4_1:11617532-11617551 Msa0535370:intron 35.0%
ATTTCCACTTTCCAATTCTC+CGG + chr4_1:11617881-11617900 None:intergenic 35.0%
TTCATCAATGATCTTGATGC+AGG - chr4_1:11617982-11618001 Msa0535370:CDS 35.0%
ATACACAGTCAATAACCAGA+TGG - chr4_1:11618032-11618051 Msa0535370:CDS 35.0%
! TCTTCTTTGTTGTACATACC+GGG + chr4_1:11618108-11618127 None:intergenic 35.0%
! CTCTTCTTTGTTGTACATAC+CGG + chr4_1:11618109-11618128 None:intergenic 35.0%
GACGCATGGAAAAATTCTAT+TGG - chr4_1:11618199-11618218 Msa0535370:intron 35.0%
!! GTGTTTGTCAAGGTATTTTC+AGG - chr4_1:11618247-11618266 Msa0535370:intron 35.0%
!! AAGGTATTTTCAGGACAGAT+GGG - chr4_1:11618256-11618275 Msa0535370:intron 35.0%
TCTTGACAATGTCATCCTTA+GGG + chr4_1:11618284-11618303 None:intergenic 35.0%
ATCTTGACAATGTCATCCTT+AGG + chr4_1:11618285-11618304 None:intergenic 35.0%
!! GCTGTTTTCATAAGCTATCA+TGG + chr4_1:11618494-11618513 None:intergenic 35.0%
CAATTCTCAAACTCATAGCA+TGG + chr4_1:11618582-11618601 None:intergenic 35.0%
CATGCTATGAGTTTGAGAAT+TGG - chr4_1:11618580-11618599 Msa0535370:intron 35.0%
!!! TAGATTTTTTTGGTGCCTTG+AGG - chr4_1:11618641-11618660 Msa0535370:intron 35.0%
!!! AGATTTTTTTGGTGCCTTGA+GGG - chr4_1:11618642-11618661 Msa0535370:intron 35.0%
AGGAATTGGATATCTGGTAT+TGG - chr4_1:11618688-11618707 Msa0535370:intron 35.0%
! TGAGGTATTTGTCAGCTAAT+TGG + chr4_1:11618849-11618868 None:intergenic 35.0%
ATCAAGACTGGAAACTTCTA+TGG - chr4_1:11618904-11618923 Msa0535370:intron 35.0%
! GATCATAGTTTTCTGCAGAA+GGG + chr4_1:11619115-11619134 None:intergenic 35.0%
TGCATTAATCTCACCACTTT+AGG - chr4_1:11619278-11619297 Msa0535370:five_prime_UTR 35.0%
GACACTTATAGAAAGCAAGT+TGG - chr4_1:11619348-11619367 Msa0535370:five_prime_UTR 35.0%
TTAAGGCGAAACCAACTTAC+TGG + chr4_1:11616978-11616997 None:intergenic 40.0%
AGAATCATCACTGTGAATCG+TGG + chr4_1:11617221-11617240 None:intergenic 40.0%
GAGCAGACTACAACAAGTTT+AGG + chr4_1:11617266-11617285 None:intergenic 40.0%
ACAAGCTTGTCCATAAATGC+AGG + chr4_1:11617545-11617564 None:intergenic 40.0%
! TTCCTCTCATTTTGGGCATT+TGG - chr4_1:11617609-11617628 Msa0535370:intron 40.0%
! TCCTCTCATTTTGGGCATTT+GGG - chr4_1:11617610-11617629 Msa0535370:intron 40.0%
TCTTTGCCAAGATGGGAATA+AGG - chr4_1:11617672-11617691 Msa0535370:intron 40.0%
CACACACCAACTATGATACT+TGG - chr4_1:11617764-11617783 Msa0535370:CDS 40.0%
CCTAAGCCAAGTATCATAGT+TGG + chr4_1:11617773-11617792 None:intergenic 40.0%
CCAACTATGATACTTGGCTT+AGG - chr4_1:11617770-11617789 Msa0535370:CDS 40.0%
ATGATACTTGGCTTAGGAGT+AGG - chr4_1:11617776-11617795 Msa0535370:CDS 40.0%
GAATTGGAAAGTGGAAATGC+CGG - chr4_1:11617883-11617902 Msa0535370:CDS 40.0%
CTTTGCCTAATCAACTTTGC+AGG + chr4_1:11617913-11617932 None:intergenic 40.0%
! CTGCAAAGTTGATTAGGCAA+AGG - chr4_1:11617911-11617930 Msa0535370:CDS 40.0%
GTTGTCAGCAATGTTCATGA+GGG + chr4_1:11618067-11618086 None:intergenic 40.0%
! ACGAATTAAGGGAGCATACA+AGG + chr4_1:11618175-11618194 None:intergenic 40.0%
TATGCTCCCTTAATTCGTGA+TGG - chr4_1:11618177-11618196 Msa0535370:intron 40.0%
ATGCGTCCATCACGAATTAA+GGG + chr4_1:11618186-11618205 None:intergenic 40.0%
!! CAAGGTATTTTCAGGACAGA+TGG - chr4_1:11618255-11618274 Msa0535370:intron 40.0%
!! AGGTATTTTCAGGACAGATG+GGG - chr4_1:11618257-11618276 Msa0535370:intron 40.0%
ATGACGATGAAGTCAGGAAT+TGG - chr4_1:11618674-11618693 Msa0535370:intron 40.0%
GAAGTCAGGAATTGGATATC+TGG - chr4_1:11618682-11618701 Msa0535370:intron 40.0%
ATCTTTGGTTGCTCGAAAGT+TGG + chr4_1:11618760-11618779 None:intergenic 40.0%
AGCTTGTCAAGAGTCATCTT+TGG + chr4_1:11618775-11618794 None:intergenic 40.0%
CTTGACAAGCTACTAGAGTA+TGG - chr4_1:11618784-11618803 Msa0535370:intron 40.0%
GATCAACAAATATGTCGCGT+CGG - chr4_1:11618986-11619005 Msa0535370:intron 40.0%
TATTGTTTGGATGCAGGACA+AGG - chr4_1:11619047-11619066 Msa0535370:intron 40.0%
! GGATCATAGTTTTCTGCAGA+AGG + chr4_1:11619116-11619135 None:intergenic 40.0%
! TTGGTCATCTGAGGTGTCAT+AGG + chr4_1:11616842-11616861 None:intergenic 45.0%
GATATCCTGTTGGTCATCTG+AGG + chr4_1:11616851-11616870 None:intergenic 45.0%
TTCCTCTGGTGATATCCTGT+TGG + chr4_1:11616861-11616880 None:intergenic 45.0%
ACAGGATATCACCAGAGGAA+AGG - chr4_1:11616861-11616880 Msa0535370:exon 45.0%
CAGGATATCACCAGAGGAAA+GGG - chr4_1:11616862-11616881 Msa0535370:exon 45.0%
TATCACCAGAGGAAAGGGAA+AGG - chr4_1:11616867-11616886 Msa0535370:exon 45.0%
CAGTGTCTTTCAAGCTCCAA+TGG - chr4_1:11616894-11616913 Msa0535370:CDS 45.0%
TTTCAAGCTCCAATGGATGC+TGG - chr4_1:11616901-11616920 Msa0535370:CDS 45.0%
TAGTGAGTTCCAGCATCCAT+TGG + chr4_1:11616913-11616932 None:intergenic 45.0%
! CCTCTCATTTTGGGCATTTG+GGG - chr4_1:11617611-11617630 Msa0535370:intron 45.0%
TGAGCTTGTCTTTGCCAAGA+TGG - chr4_1:11617664-11617683 Msa0535370:intron 45.0%
GAGCTTGTCTTTGCCAAGAT+GGG - chr4_1:11617665-11617684 Msa0535370:intron 45.0%
AGCCCTATAATGATGAGTGC+CGG - chr4_1:11617859-11617878 Msa0535370:CDS 45.0%
CTCCGGCACTCATCATTATA+GGG + chr4_1:11617864-11617883 None:intergenic 45.0%
TCTCCGGCACTCATCATTAT+AGG + chr4_1:11617865-11617884 None:intergenic 45.0%
!! AATGATGAGTGCCGGAGAAT+TGG - chr4_1:11617867-11617886 Msa0535370:CDS 45.0%
AATCAACTTTGCAGGCTCTC+CGG + chr4_1:11617905-11617924 None:intergenic 45.0%
! GAGAGCCTGCAAAGTTGATT+AGG - chr4_1:11617905-11617924 Msa0535370:CDS 45.0%
GCGTCCGACATTATCAGAAA+AGG - chr4_1:11617943-11617962 Msa0535370:CDS 45.0%
AATGATCTTGATGCAGGAGC+TGG - chr4_1:11617988-11618007 Msa0535370:CDS 45.0%
TGAGGGTTGCATTTACCATC+TGG + chr4_1:11618050-11618069 None:intergenic 45.0%
GGTTGTCAGCAATGTTCATG+AGG + chr4_1:11618068-11618087 None:intergenic 45.0%
CGGGAAGTTGAACATTGGTA+GGG + chr4_1:11618089-11618108 None:intergenic 45.0%
CCTACCAATGTTCAACTTCC+CGG - chr4_1:11618087-11618106 Msa0535370:CDS 45.0%
CATACCGGGAAGTTGAACAT+TGG + chr4_1:11618094-11618113 None:intergenic 45.0%
CATTGCCAGTGACGATGATA+GGG + chr4_1:11618143-11618162 None:intergenic 45.0%
TCATTGCCAGTGACGATGAT+AGG + chr4_1:11618144-11618163 None:intergenic 45.0%
CATGCGTCCATCACGAATTA+AGG + chr4_1:11618187-11618206 None:intergenic 45.0%
!! CTTAATTCGTGATGGACGCA+TGG - chr4_1:11618185-11618204 Msa0535370:intron 45.0%
!! GATAGGGTTGGTGTTTGTCA+AGG - chr4_1:11618237-11618256 Msa0535370:intron 45.0%
AAGATTGTCGACACATTCCC+TGG - chr4_1:11618300-11618319 Msa0535370:intron 45.0%
ACATTCCCTGGACAGTCAAT+TGG - chr4_1:11618312-11618331 Msa0535370:intron 45.0%
GCTTACCAATTGACTGTCCA+GGG + chr4_1:11618320-11618339 None:intergenic 45.0%
GAGTGTATGACGATGAAGTC+AGG - chr4_1:11618668-11618687 Msa0535370:intron 45.0%
!! TTGGATATCTGGTATTGGCG+TGG - chr4_1:11618693-11618712 Msa0535370:intron 45.0%
TTTGGTTGCTCGAAAGTTGG+AGG + chr4_1:11618757-11618776 None:intergenic 45.0%
AGCTGACAAATACCTCAGTG+AGG - chr4_1:11618852-11618871 Msa0535370:intron 45.0%
CGTCGGTCTTGACAATAACT+TGG - chr4_1:11619003-11619022 Msa0535370:intron 45.0%
TCGCGGAACTCGCTTTCTTA+AGG + chr4_1:11616720-11616739 None:intergenic 50.0%
! ACTCTTCTGGCTGGAAATCG+CGG + chr4_1:11616737-11616756 None:intergenic 50.0%
TCCAGCCAGAAGAGTTTGTC+TGG - chr4_1:11616742-11616761 Msa0535370:three_prime_UTR 50.0%
ACCAGACAAACTCTTCTGGC+TGG + chr4_1:11616746-11616765 None:intergenic 50.0%
TGAGGAGAAGCAGACCTCAA+AGG - chr4_1:11616798-11616817 Msa0535370:three_prime_UTR 50.0%
AGCAGACCTCAAAGGACAGA+TGG - chr4_1:11616806-11616825 Msa0535370:three_prime_UTR 50.0%
GCAGACCTCAAAGGACAGAT+GGG - chr4_1:11616807-11616826 Msa0535370:three_prime_UTR 50.0%
TGACACCTCAGATGACCAAC+AGG - chr4_1:11616843-11616862 Msa0535370:three_prime_UTR 50.0%
GACCAACAGGATATCACCAG+AGG - chr4_1:11616856-11616875 Msa0535370:exon 50.0%
ACCAACTTACTGGCGAAGAC+CGG + chr4_1:11616968-11616987 None:intergenic 50.0%
CCCCAAATGCCCAAAATGAG+AGG + chr4_1:11617614-11617633 None:intergenic 50.0%
! CTCATTTTGGGCATTTGGGG+AGG - chr4_1:11617614-11617633 Msa0535370:intron 50.0%
ATTTGGGGAGGCAAAGGTCA+AGG - chr4_1:11617626-11617645 Msa0535370:intron 50.0%
! AGTGCCGGAGAATTGGAAAG+TGG - chr4_1:11617874-11617893 Msa0535370:CDS 50.0%
!! GATTAGGCAAAGGTACCGTG+AGG - chr4_1:11617921-11617940 Msa0535370:CDS 50.0%
! TGATAATGTCGGACGCCTCA+CGG + chr4_1:11617939-11617958 None:intergenic 50.0%
TGATGCAGGAGCTGGTAGAA+TGG - chr4_1:11617996-11618015 Msa0535370:CDS 50.0%
GATGCAGGAGCTGGTAGAAT+GGG - chr4_1:11617997-11618016 Msa0535370:CDS 50.0%
CCGGGAAGTTGAACATTGGT+AGG + chr4_1:11618090-11618109 None:intergenic 50.0%
AGTGACGATGATAGGGACAC+GGG + chr4_1:11618136-11618155 None:intergenic 50.0%
CAGTGACGATGATAGGGACA+CGG + chr4_1:11618137-11618156 None:intergenic 50.0%
CCAACCCTATCATCACGAGT+AGG + chr4_1:11618228-11618247 None:intergenic 50.0%
CCTACTCGTGATGATAGGGT+TGG - chr4_1:11618225-11618244 Msa0535370:intron 50.0%
GGCTTACCAATTGACTGTCC+AGG + chr4_1:11618321-11618340 None:intergenic 50.0%
GTCATACACTCTAGCCCTCA+AGG + chr4_1:11618659-11618678 None:intergenic 50.0%
!! GGTATTGGCGTGGAGAGTAT+TGG - chr4_1:11618703-11618722 Msa0535370:intron 50.0%
GTTCCTTTACCTGTCCCAGA+AGG - chr4_1:11619080-11619099 Msa0535370:CDS 50.0%
!! CAACCTTCTGGGACAGGTAA+AGG + chr4_1:11619086-11619105 None:intergenic 50.0%
GCAACTTCCATCGTCACTTC+TGG + chr4_1:11619144-11619163 None:intergenic 50.0%
ACCTCAAAGGACAGATGGGC+TGG - chr4_1:11616811-11616830 Msa0535370:three_prime_UTR 55.0%
TCCAGCCCATCTGTCCTTTG+AGG + chr4_1:11616815-11616834 None:intergenic 55.0%
CACCAGAGGAAAGGGAAAGG+TGG - chr4_1:11616870-11616889 Msa0535370:exon 55.0%
GTCCACCTTTCCCTTTCCTC+TGG + chr4_1:11616875-11616894 None:intergenic 55.0%
ACCGGTCTTCGCCAGTAAGT+TGG - chr4_1:11616964-11616983 Msa0535370:CDS 55.0%
TTGGGCATTTGGGGAGGCAA+AGG - chr4_1:11617620-11617639 Msa0535370:intron 55.0%
CGTGTCCCTATCATCGTCAC+TGG - chr4_1:11618135-11618154 Msa0535370:CDS 55.0%
GGCGCCTACTCGTGATGATA+GGG - chr4_1:11618221-11618240 Msa0535370:intron 55.0%
TACCTCAGTGAGGCAGCTCT+TGG - chr4_1:11618862-11618881 Msa0535370:intron 55.0%
AAGGGTCAGCGCAACCTTCT+GGG + chr4_1:11619097-11619116 None:intergenic 55.0%
! CCATCGTCACTTCTGGCTGT+CGG + chr4_1:11619137-11619156 None:intergenic 55.0%
GCAGGAGCTGGTAGAATGGG+TGG - chr4_1:11618000-11618019 Msa0535370:CDS 60.0%
GGGCGCCTACTCGTGATGAT+AGG - chr4_1:11618220-11618239 Msa0535370:intron 60.0%
CAGGACAGATGGGGTCCCTA+AGG - chr4_1:11618266-11618285 Msa0535370:intron 60.0%
CTCCAAGAGCTGCCTCACTG+AGG + chr4_1:11618867-11618886 None:intergenic 60.0%
!! TCAGCGCAACCTTCTGGGAC+AGG + chr4_1:11619092-11619111 None:intergenic 60.0%
GAAGGGTCAGCGCAACCTTC+TGG + chr4_1:11619098-11619117 None:intergenic 60.0%
CCGACAGCCAGAAGTGACGA+TGG - chr4_1:11619134-11619153 Msa0535370:exon 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 11616652 11619397 11616652 ID=Msa0535370;Name=Msa0535370
chr4_1 mRNA 11616652 11619397 11616652 ID=Msa0535370-mRNA-1;Parent=Msa0535370;Name=Msa0535370-mRNA-1;_AED=0.04;_eAED=0.04;_QI=261|1|1|1|1|1|5|219|375
chr4_1 exon 11616652 11616986 11616652 ID=Msa0535370-mRNA-1:exon:3734;Parent=Msa0535370-mRNA-1
chr4_1 exon 11617124 11617405 11617124 ID=Msa0535370-mRNA-1:exon:3733;Parent=Msa0535370-mRNA-1
chr4_1 exon 11617716 11618188 11617716 ID=Msa0535370-mRNA-1:exon:3732;Parent=Msa0535370-mRNA-1
chr4_1 exon 11618356 11618547 11618356 ID=Msa0535370-mRNA-1:exon:3731;Parent=Msa0535370-mRNA-1
chr4_1 exon 11619072 11619397 11619072 ID=Msa0535370-mRNA-1:exon:3730;Parent=Msa0535370-mRNA-1
chr4_1 five_prime_UTR 11619137 11619397 11619137 ID=Msa0535370-mRNA-1:five_prime_utr;Parent=Msa0535370-mRNA-1
chr4_1 CDS 11619072 11619136 11619072 ID=Msa0535370-mRNA-1:cds;Parent=Msa0535370-mRNA-1
chr4_1 CDS 11618356 11618547 11618356 ID=Msa0535370-mRNA-1:cds;Parent=Msa0535370-mRNA-1
chr4_1 CDS 11617716 11618188 11617716 ID=Msa0535370-mRNA-1:cds;Parent=Msa0535370-mRNA-1
chr4_1 CDS 11617124 11617405 11617124 ID=Msa0535370-mRNA-1:cds;Parent=Msa0535370-mRNA-1
chr4_1 CDS 11616871 11616986 11616871 ID=Msa0535370-mRNA-1:cds;Parent=Msa0535370-mRNA-1
chr4_1 three_prime_UTR 11616652 11616870 11616652 ID=Msa0535370-mRNA-1:three_prime_utr;Parent=Msa0535370-mRNA-1
Gene Sequence

>Msa0535370

ATGGATGCTGGAACTCACTATGCTGTCATGAGCTCCTATGATTATATCAGTACCGGTCTTCGCCAGTACAATATGGACAACACAATAGATGGCTTGTATATTGCACCTGCATTTATGGACAAGCTTGTTGTTCACATCACTAAGAACTTTCTCAACTTGCCAAATATTAAGATTCCTCTCATTTTGGGCATTTGGGGAGGCAAAGGTCAAGGAAAGTCATTTCAATGTGAGCTTGTCTTTGCCAAGATGGGAATAAGCCCTATAATGATGAGTGCCGGAGAATTGGAAAGTGGAAATGCCGGAGAGCCTGCAAAGTTGATTAGGCAAAGGTACCGTGAGGCGTCCGACATTATCAGAAAAGGAAAGATGTGCTGTCTCTTCATCAATGATCTTGATGCAGGAGCTGGTAGAATGGGTGGCACTACTCAATACACAGTCAATAACCAGATGGTAAATGCAACCCTCATGAACATTGCTGACAACCCTACCAATGTTCAACTTCCCGGTATGTACAACAAAGAAGAGAATGCCCGTGTCCCTATCATCGTCACTGGCAATGACTTCTCTACCTTGTATGCTCCCTTAATTCGTGATGGACGCATGGAAAAATTCTATTGGGCGCCTACTCGTGATGATAGGGTTGGTGTTTGTCAAGGTATTTTCAGGACAGATGGGGTCCCTAAGGATGACATTGTCAAGATTGTCGACACATTCCCTGGACAGTCAATTGATTTTTTTGGTGCCTTGAGGGCTAGAGTGTATGACGATGAAGTCAGGAATTGGATATCTGGTATTGGCGTGGAGAGTATTGGAAAAAGACTTGTGAATTCAAAAGAGGGACCTCCAACTTTCGAGCAACCAAAGATGACTCTTGACAAGCTACTAGAGTATGGAAATATGCTTGTCCAAGAACAAGAGAATGTTAAGAGGGTCCAATTAGCTGACAAATACCTCAGTGAGGCAGCTCTTGGAGATGCAAATAATGATGCTATCAAGACTGGAAACTTCTATGGACAAGGAGCTCAGCAAGTTCCTTTACCTGTCCCAGAAGGTTGCGCTGACCCTTCTGCAGAAAACTATGATCCGACAGCCAGAAGTGACGATGGAAGTTGCACATACACTTTTTAG
Protein sequence

>Msa0535370

MDAGTHYAVMSSYDYISTGLRQYNMDNTIDGLYIAPAFMDKLVVHITKNFLNLPNIKIPLILGIWGGKGQGKSFQCELVFAKMGISPIMMSAGELESGNAGEPAKLIRQRYREASDIIRKGKMCCLFINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENARVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTRDDRVGVCQGIFRTDGVPKDDIVKIVDTFPGQSIDFFGALRARVYDDEVRNWISGIGVESIGKRLVNSKEGPPTFEQPKMTLDKLLEYGNMLVQEQENVKRVQLADKYLSEAALGDANNDAIKTGNFYGQGAQQVPLPVPEGCADPSAENYDPTARSDDGSCTYTF*