AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0535820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0535820 MtrunA17_Chr4g0009801 97.895 95 2 0 1 95 1 95 2.07e-66 194
Msa0535820 MtrunA17_Chr4g0031531 59.140 93 38 0 1 93 1 93 5.98e-41 130
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0535820 AT3G46560.1 58.065 93 39 0 1 93 1 93 1.34e-38 124

Find 14 sgRNAs with CRISPR-Local

Find 332 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATTGTTTATGTAGTTTCA+TGG 0.323073 4_1:-12443699 Msa0535820:three_prime_UTR
CTACAACAGACAAACAATAA+TGG 0.424423 4_1:-12443883 Msa0535820:exon
TAAGAAAAGAATGACTACTA+TGG 0.430118 4_1:-12446552 Msa0535820:CDS
AGCTGGAATTTCTAAAGGTA+AGG 0.435045 4_1:-12443749 Msa0535820:three_prime_UTR
TGTCTGTTGTAGATGCTCCT+TGG 0.470723 4_1:+12443894 None:intergenic
ATACAAATTATGTAATATCA+AGG 0.474461 4_1:-12443617 None:intergenic
CTGAGGACACATGTTTCCTC+TGG 0.481217 4_1:+12443977 None:intergenic
ACATGTGTCCTCAGATGTGC+TGG 0.504009 4_1:-12443968 Msa0535820:CDS
ATAGATAGAATAAAATGAGC+TGG 0.506855 4_1:-12443766 Msa0535820:three_prime_UTR
ATTGCGTCAATTCATTCTAT+CGG 0.526040 4_1:-12444015 Msa0535820:CDS
AAATGAGCTGGAATTTCTAA+AGG 0.527189 4_1:-12443754 Msa0535820:three_prime_UTR
AACAGCTTCAAACTCGTGAT+AGG 0.528764 4_1:-12446526 Msa0535820:intron
GATGTGCTGGAAAATACTTG+AGG 0.670940 4_1:-12443955 Msa0535820:CDS
GAGTTCGTTGAACAAGCCAG+AGG 0.696062 4_1:-12443993 Msa0535820:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAAATCATATATAGAT+TGG - chr4_1:12443782-12443801 Msa0535820:three_prime_UTR 10.0%
!! ATAAAAATCATATATAGATT+GGG - chr4_1:12443783-12443802 Msa0535820:three_prime_UTR 10.0%
!! AATAAAAATCATATATAGAT+TGG - chr4_1:12443782-12443801 Msa0535820:three_prime_UTR 10.0%
!! ATAAAAATCATATATAGATT+GGG - chr4_1:12443783-12443802 Msa0535820:three_prime_UTR 10.0%
!! TAAAAATCATATATAGATTG+GGG - chr4_1:12443784-12443803 Msa0535820:three_prime_UTR 15.0%
!! TTTATTTATTACTAGAGAAA+TGG + chr4_1:12444191-12444210 None:intergenic 15.0%
!! GGTAAAAAATAATGAAAAAA+TGG - chr4_1:12444383-12444402 Msa0535820:intron 15.0%
!! AAAAAAAAAAACATGTTAAC+TGG - chr4_1:12445586-12445605 Msa0535820:intron 15.0%
!!! TCATTTTATTCTATCTATTA+AGG + chr4_1:12446493-12446512 None:intergenic 15.0%
!! TAAAAATCATATATAGATTG+GGG - chr4_1:12443784-12443803 Msa0535820:three_prime_UTR 15.0%
!! TTTATTTATTACTAGAGAAA+TGG + chr4_1:12444191-12444210 None:intergenic 15.0%
!! GGTAAAAAATAATGAAAAAA+TGG - chr4_1:12444383-12444402 Msa0535820:intron 15.0%
!! AAAAAAAAAAACATGTTAAC+TGG - chr4_1:12445586-12445605 Msa0535820:intron 15.0%
!!! TCATTTTATTCTATCTATTA+AGG + chr4_1:12446493-12446512 None:intergenic 15.0%
!! GTAAACATATTAAGCATTAT+GGG - chr4_1:12443941-12443960 Msa0535820:CDS 20.0%
!!! ACTGTTTTTATTATCTATTC+TGG - chr4_1:12444242-12444261 Msa0535820:intron 20.0%
!! TTTAACGTAATTATATGTGT+TGG - chr4_1:12444411-12444430 Msa0535820:intron 20.0%
!! TAATTCAATGTATTTAATCG+TGG - chr4_1:12444576-12444595 Msa0535820:intron 20.0%
!! ATTGTCTATAATATCAAAGT+TGG + chr4_1:12444610-12444629 None:intergenic 20.0%
!! TCTATTGTGTTTATAGTAAA+TGG - chr4_1:12444985-12445004 Msa0535820:intron 20.0%
!! ATAACTAAAAACACAATACA+GGG + chr4_1:12445044-12445063 None:intergenic 20.0%
!! AATAACTAAAAACACAATAC+AGG + chr4_1:12445045-12445064 None:intergenic 20.0%
!! TTTAGTTATTCCATATCAAA+TGG - chr4_1:12445055-12445074 Msa0535820:intron 20.0%
!!! TTTAACTTTAACTGTACATT+TGG - chr4_1:12445523-12445542 Msa0535820:intron 20.0%
!!! CTTTTATATGTGTACAATTT+CGG + chr4_1:12446014-12446033 None:intergenic 20.0%
!! GTAAACATATTAAGCATTAT+GGG - chr4_1:12443941-12443960 Msa0535820:CDS 20.0%
!!! ACTGTTTTTATTATCTATTC+TGG - chr4_1:12444242-12444261 Msa0535820:intron 20.0%
!! TTTAACGTAATTATATGTGT+TGG - chr4_1:12444411-12444430 Msa0535820:intron 20.0%
!! TAATTCAATGTATTTAATCG+TGG - chr4_1:12444576-12444595 Msa0535820:intron 20.0%
!! ATTGTCTATAATATCAAAGT+TGG + chr4_1:12444610-12444629 None:intergenic 20.0%
!! TCTATTGTGTTTATAGTAAA+TGG - chr4_1:12444985-12445004 Msa0535820:intron 20.0%
!! ATAACTAAAAACACAATACA+GGG + chr4_1:12445044-12445063 None:intergenic 20.0%
!! AATAACTAAAAACACAATAC+AGG + chr4_1:12445045-12445064 None:intergenic 20.0%
!! TTTAGTTATTCCATATCAAA+TGG - chr4_1:12445055-12445074 Msa0535820:intron 20.0%
!!! TTTAACTTTAACTGTACATT+TGG - chr4_1:12445523-12445542 Msa0535820:intron 20.0%
!!! CTTTTATATGTGTACAATTT+CGG + chr4_1:12446014-12446033 None:intergenic 20.0%
!! AAACAGTTACAGAGATTTTT+GGG + chr4_1:12443628-12443647 None:intergenic 25.0%
!! AAAACAGTTACAGAGATTTT+TGG + chr4_1:12443629-12443648 None:intergenic 25.0%
!!! TGTAACTGTTTTTTTGTTGT+TGG - chr4_1:12443637-12443656 Msa0535820:three_prime_UTR 25.0%
!!! TGTTTTTTTGTTGTTGGAAA+TGG - chr4_1:12443643-12443662 Msa0535820:three_prime_UTR 25.0%
!! GAAATGGATAAGAACATTTT+TGG - chr4_1:12443659-12443678 Msa0535820:three_prime_UTR 25.0%
!! ATTCTTTTCTTATCTTCTTC+AGG + chr4_1:12443701-12443720 None:intergenic 25.0%
! TAAGAAAAGAATGACTACTA+TGG - chr4_1:12443709-12443728 Msa0535820:three_prime_UTR 25.0%
! TATGTTTACCCAAACAAATA+GGG + chr4_1:12443930-12443949 None:intergenic 25.0%
! ATATGTTTACCCAAACAAAT+AGG + chr4_1:12443931-12443950 None:intergenic 25.0%
! GGTAAACATATTAAGCATTA+TGG - chr4_1:12443940-12443959 Msa0535820:CDS 25.0%
! TATTATTCATAAGCTTGTCA+AGG + chr4_1:12444083-12444102 None:intergenic 25.0%
! TCATGGTCAAATATTTATGA+TGG - chr4_1:12444793-12444812 Msa0535820:intron 25.0%
!!! TTAAAGCATTCATTTTGATG+AGG - chr4_1:12444916-12444935 Msa0535820:intron 25.0%
! TTACTATAAACACAATAGAG+AGG + chr4_1:12444984-12445003 None:intergenic 25.0%
! TTCTACTATGTTATAGTGTT+GGG + chr4_1:12445287-12445306 None:intergenic 25.0%
! ATTCTACTATGTTATAGTGT+TGG + chr4_1:12445288-12445307 None:intergenic 25.0%
! ATCAATCAACTCAATTACTA+TGG + chr4_1:12445625-12445644 None:intergenic 25.0%
!!! TAGTAATTGAGTTGATTGAT+TGG - chr4_1:12445625-12445644 Msa0535820:intron 25.0%
! ACTATTTAGTACAGAATGTT+TGG - chr4_1:12445781-12445800 Msa0535820:intron 25.0%
!! AAATTTTCAGATATCAACCA+AGG - chr4_1:12446350-12446369 Msa0535820:intron 25.0%
!!! TCTTTTTTACTGTCAATAAC+TGG - chr4_1:12446439-12446458 Msa0535820:intron 25.0%
!!! CTTTTTTACTGTCAATAACT+GGG - chr4_1:12446440-12446459 Msa0535820:intron 25.0%
! ATAGATAGAATAAAATGAGC+TGG - chr4_1:12446495-12446514 Msa0535820:intron 25.0%
! CTATTGTTTATGTAGTTTCA+TGG - chr4_1:12446562-12446581 Msa0535820:CDS 25.0%
! TAACAAATGAAAAAAGCGAA+TGG + chr4_1:12446610-12446629 None:intergenic 25.0%
!! AAACAGTTACAGAGATTTTT+GGG + chr4_1:12443628-12443647 None:intergenic 25.0%
!! AAAACAGTTACAGAGATTTT+TGG + chr4_1:12443629-12443648 None:intergenic 25.0%
!!! TGTAACTGTTTTTTTGTTGT+TGG - chr4_1:12443637-12443656 Msa0535820:three_prime_UTR 25.0%
!!! TGTTTTTTTGTTGTTGGAAA+TGG - chr4_1:12443643-12443662 Msa0535820:three_prime_UTR 25.0%
!! GAAATGGATAAGAACATTTT+TGG - chr4_1:12443659-12443678 Msa0535820:three_prime_UTR 25.0%
!! ATTCTTTTCTTATCTTCTTC+AGG + chr4_1:12443701-12443720 None:intergenic 25.0%
! TAAGAAAAGAATGACTACTA+TGG - chr4_1:12443709-12443728 Msa0535820:three_prime_UTR 25.0%
! TATGTTTACCCAAACAAATA+GGG + chr4_1:12443930-12443949 None:intergenic 25.0%
! ATATGTTTACCCAAACAAAT+AGG + chr4_1:12443931-12443950 None:intergenic 25.0%
! GGTAAACATATTAAGCATTA+TGG - chr4_1:12443940-12443959 Msa0535820:CDS 25.0%
! TATTATTCATAAGCTTGTCA+AGG + chr4_1:12444083-12444102 None:intergenic 25.0%
! TCATGGTCAAATATTTATGA+TGG - chr4_1:12444793-12444812 Msa0535820:intron 25.0%
!!! TTAAAGCATTCATTTTGATG+AGG - chr4_1:12444916-12444935 Msa0535820:intron 25.0%
! TTACTATAAACACAATAGAG+AGG + chr4_1:12444984-12445003 None:intergenic 25.0%
! TTCTACTATGTTATAGTGTT+GGG + chr4_1:12445287-12445306 None:intergenic 25.0%
! ATTCTACTATGTTATAGTGT+TGG + chr4_1:12445288-12445307 None:intergenic 25.0%
! ATCAATCAACTCAATTACTA+TGG + chr4_1:12445625-12445644 None:intergenic 25.0%
!!! TAGTAATTGAGTTGATTGAT+TGG - chr4_1:12445625-12445644 Msa0535820:intron 25.0%
! ACTATTTAGTACAGAATGTT+TGG - chr4_1:12445781-12445800 Msa0535820:intron 25.0%
!! AAATTTTCAGATATCAACCA+AGG - chr4_1:12446350-12446369 Msa0535820:intron 25.0%
!!! TCTTTTTTACTGTCAATAAC+TGG - chr4_1:12446439-12446458 Msa0535820:intron 25.0%
!!! CTTTTTTACTGTCAATAACT+GGG - chr4_1:12446440-12446459 Msa0535820:intron 25.0%
! ATAGATAGAATAAAATGAGC+TGG - chr4_1:12446495-12446514 Msa0535820:intron 25.0%
! CTATTGTTTATGTAGTTTCA+TGG - chr4_1:12446562-12446581 Msa0535820:CDS 25.0%
! TAACAAATGAAAAAAGCGAA+TGG + chr4_1:12446610-12446629 None:intergenic 25.0%
! AACAGTTACAGAGATTTTTG+GGG + chr4_1:12443627-12443646 None:intergenic 30.0%
ATGTTATGATGTGCTGAAAA+TGG - chr4_1:12443827-12443846 Msa0535820:three_prime_UTR 30.0%
!! TAGAATGTGTTGATTCTAGT+TGG - chr4_1:12443896-12443915 Msa0535820:CDS 30.0%
CAAATATGCCCTATTTGTTT+GGG - chr4_1:12443919-12443938 Msa0535820:CDS 30.0%
! TCGTTTGGATTGACTTATTT+CGG - chr4_1:12443965-12443984 Msa0535820:CDS 30.0%
CAAAAATAAGCCATTCCAAA+CGG - chr4_1:12444126-12444145 Msa0535820:intron 30.0%
AAAAATAAGCCATTCCAAAC+GGG - chr4_1:12444127-12444146 Msa0535820:intron 30.0%
CTATATGAGAAGTCCTTAAA+GGG + chr4_1:12444153-12444172 None:intergenic 30.0%
TCTATATGAGAAGTCCTTAA+AGG + chr4_1:12444154-12444173 None:intergenic 30.0%
TATCTATTCTGGACAGTTTA+TGG - chr4_1:12444253-12444272 Msa0535820:intron 30.0%
ATCTATTCTGGACAGTTTAT+GGG - chr4_1:12444254-12444273 Msa0535820:intron 30.0%
AGTTAGTAGATATGCTTGAA+TGG - chr4_1:12444536-12444555 Msa0535820:intron 30.0%
GTGTATTCATGATCTAAACA+GGG + chr4_1:12444896-12444915 None:intergenic 30.0%
AGTGTATTCATGATCTAAAC+AGG + chr4_1:12444897-12444916 None:intergenic 30.0%
TACACATTCTTATAGTCTAG+AGG + chr4_1:12444959-12444978 None:intergenic 30.0%
ACTCAAAACCTTAAGACATT+GGG + chr4_1:12445189-12445208 None:intergenic 30.0%
AACTCAAAACCTTAAGACAT+TGG + chr4_1:12445190-12445209 None:intergenic 30.0%
! ATTTTCAGCATTGTCAATTG+TGG + chr4_1:12445433-12445452 None:intergenic 30.0%
ACAATTGACAATGCTGAAAA+TGG - chr4_1:12445432-12445451 Msa0535820:intron 30.0%
ATCAGCATTAACATGTTAAC+TGG - chr4_1:12445552-12445571 Msa0535820:intron 30.0%
! AACATGTTAACTGGTGATTT+TGG - chr4_1:12445595-12445614 Msa0535820:intron 30.0%
CATGCAATGTTGTTAAATAG+CGG - chr4_1:12445693-12445712 Msa0535820:intron 30.0%
TGAACAAATCACTATTGTTC+CGG - chr4_1:12445753-12445772 Msa0535820:intron 30.0%
CTGTACTAAATAGTGTATTC+CGG + chr4_1:12445775-12445794 None:intergenic 30.0%
TACAGAATGTTTGGAAATAG+CGG - chr4_1:12445790-12445809 Msa0535820:intron 30.0%
ATAGTGCTATAGTAAATGAC+AGG + chr4_1:12445908-12445927 None:intergenic 30.0%
!! TATTGACAGTATTGATCACA+TGG - chr4_1:12445971-12445990 Msa0535820:intron 30.0%
! TGAGTGCAAAAGTTTTCTTT+TGG - chr4_1:12446082-12446101 Msa0535820:intron 30.0%
ATTGCGTCAATTCATTCTAT+CGG - chr4_1:12446246-12446265 Msa0535820:intron 30.0%
CTACAACAGACAAACAATAA+TGG - chr4_1:12446378-12446397 Msa0535820:intron 30.0%
AAATGAGCTGGAATTTCTAA+AGG - chr4_1:12446507-12446526 Msa0535820:intron 30.0%
! AACAGTTACAGAGATTTTTG+GGG + chr4_1:12443627-12443646 None:intergenic 30.0%
ATGTTATGATGTGCTGAAAA+TGG - chr4_1:12443827-12443846 Msa0535820:three_prime_UTR 30.0%
!! TAGAATGTGTTGATTCTAGT+TGG - chr4_1:12443896-12443915 Msa0535820:CDS 30.0%
CAAATATGCCCTATTTGTTT+GGG - chr4_1:12443919-12443938 Msa0535820:CDS 30.0%
! TCGTTTGGATTGACTTATTT+CGG - chr4_1:12443965-12443984 Msa0535820:CDS 30.0%
CAAAAATAAGCCATTCCAAA+CGG - chr4_1:12444126-12444145 Msa0535820:intron 30.0%
AAAAATAAGCCATTCCAAAC+GGG - chr4_1:12444127-12444146 Msa0535820:intron 30.0%
CTATATGAGAAGTCCTTAAA+GGG + chr4_1:12444153-12444172 None:intergenic 30.0%
TCTATATGAGAAGTCCTTAA+AGG + chr4_1:12444154-12444173 None:intergenic 30.0%
TATCTATTCTGGACAGTTTA+TGG - chr4_1:12444253-12444272 Msa0535820:intron 30.0%
ATCTATTCTGGACAGTTTAT+GGG - chr4_1:12444254-12444273 Msa0535820:intron 30.0%
AGTTAGTAGATATGCTTGAA+TGG - chr4_1:12444536-12444555 Msa0535820:intron 30.0%
GTGTATTCATGATCTAAACA+GGG + chr4_1:12444896-12444915 None:intergenic 30.0%
AGTGTATTCATGATCTAAAC+AGG + chr4_1:12444897-12444916 None:intergenic 30.0%
TACACATTCTTATAGTCTAG+AGG + chr4_1:12444959-12444978 None:intergenic 30.0%
ACTCAAAACCTTAAGACATT+GGG + chr4_1:12445189-12445208 None:intergenic 30.0%
AACTCAAAACCTTAAGACAT+TGG + chr4_1:12445190-12445209 None:intergenic 30.0%
! ATTTTCAGCATTGTCAATTG+TGG + chr4_1:12445433-12445452 None:intergenic 30.0%
ACAATTGACAATGCTGAAAA+TGG - chr4_1:12445432-12445451 Msa0535820:intron 30.0%
ATCAGCATTAACATGTTAAC+TGG - chr4_1:12445552-12445571 Msa0535820:intron 30.0%
! AACATGTTAACTGGTGATTT+TGG - chr4_1:12445595-12445614 Msa0535820:intron 30.0%
CATGCAATGTTGTTAAATAG+CGG - chr4_1:12445693-12445712 Msa0535820:intron 30.0%
TGAACAAATCACTATTGTTC+CGG - chr4_1:12445753-12445772 Msa0535820:intron 30.0%
CTGTACTAAATAGTGTATTC+CGG + chr4_1:12445775-12445794 None:intergenic 30.0%
TACAGAATGTTTGGAAATAG+CGG - chr4_1:12445790-12445809 Msa0535820:intron 30.0%
ATAGTGCTATAGTAAATGAC+AGG + chr4_1:12445908-12445927 None:intergenic 30.0%
!! TATTGACAGTATTGATCACA+TGG - chr4_1:12445971-12445990 Msa0535820:intron 30.0%
! TGAGTGCAAAAGTTTTCTTT+TGG - chr4_1:12446082-12446101 Msa0535820:intron 30.0%
ATTGCGTCAATTCATTCTAT+CGG - chr4_1:12446246-12446265 Msa0535820:intron 30.0%
CTACAACAGACAAACAATAA+TGG - chr4_1:12446378-12446397 Msa0535820:intron 30.0%
AAATGAGCTGGAATTTCTAA+AGG - chr4_1:12446507-12446526 Msa0535820:intron 30.0%
GCAAATATGCCCTATTTGTT+TGG - chr4_1:12443918-12443937 Msa0535820:CDS 35.0%
! GACATGTCATAAGTTGTTTC+AGG + chr4_1:12444286-12444305 None:intergenic 35.0%
!!! TATAGCACTGACACTTTTGA+TGG + chr4_1:12444493-12444512 None:intergenic 35.0%
TAATATCAAAGTTGGCATCG+TGG + chr4_1:12444602-12444621 None:intergenic 35.0%
ATGTTGTCAAATATCCACCA+TGG - chr4_1:12444628-12444647 Msa0535820:intron 35.0%
! TTATCGATTGAGTACCATGA+TGG + chr4_1:12444757-12444776 None:intergenic 35.0%
CATGAAAAAACATGACGTTG+CGG - chr4_1:12444839-12444858 Msa0535820:intron 35.0%
TGAATTAACCCATACTCGAA+TGG + chr4_1:12445011-12445030 None:intergenic 35.0%
TATAGTCCGCTATATGACAA+CGG + chr4_1:12445082-12445101 None:intergenic 35.0%
!! TCCACTTTTCTAAGCAATGT+GGG + chr4_1:12445133-12445152 None:intergenic 35.0%
CCTTAAGACATTGGGTTTAT+TGG + chr4_1:12445181-12445200 None:intergenic 35.0%
CCAATAAACCCAATGTCTTA+AGG - chr4_1:12445178-12445197 Msa0535820:intron 35.0%
!!! TAAGGTTTTGAGTTGAACTG+TGG - chr4_1:12445196-12445215 Msa0535820:intron 35.0%
ATGATTCACTATTAGTGCTG+CGG - chr4_1:12445330-12445349 Msa0535820:intron 35.0%
! ATGTCAAACAGTCGTCATTT+TGG - chr4_1:12445356-12445375 Msa0535820:intron 35.0%
GCATTGTTCACATTCATCTA+AGG + chr4_1:12445397-12445416 None:intergenic 35.0%
GCTACCATGTTATAGTATTG+CGG - chr4_1:12445726-12445745 Msa0535820:intron 35.0%
AACTCCGCAATACTATAACA+TGG + chr4_1:12445733-12445752 None:intergenic 35.0%
ACACTATGCAATAGTGCTAT+AGG + chr4_1:12445826-12445845 None:intergenic 35.0%
ACTATTGCATAGTGTTGTAG+CGG - chr4_1:12445831-12445850 Msa0535820:intron 35.0%
TGATCACATGGCATTTACTA+TGG - chr4_1:12445983-12446002 Msa0535820:intron 35.0%
TGGTTACTTCACTCTTTCTT+TGG - chr4_1:12446102-12446121 Msa0535820:intron 35.0%
TCCAACAAAATTGCAGTCTT+AGG - chr4_1:12446189-12446208 Msa0535820:intron 35.0%
! ACCTAAGACTGCAATTTTGT+TGG + chr4_1:12446193-12446212 None:intergenic 35.0%
! AGCTGGAATTTCTAAAGGTA+AGG - chr4_1:12446512-12446531 Msa0535820:intron 35.0%
GCAAATATGCCCTATTTGTT+TGG - chr4_1:12443918-12443937 Msa0535820:CDS 35.0%
! GACATGTCATAAGTTGTTTC+AGG + chr4_1:12444286-12444305 None:intergenic 35.0%
!!! TATAGCACTGACACTTTTGA+TGG + chr4_1:12444493-12444512 None:intergenic 35.0%
TAATATCAAAGTTGGCATCG+TGG + chr4_1:12444602-12444621 None:intergenic 35.0%
ATGTTGTCAAATATCCACCA+TGG - chr4_1:12444628-12444647 Msa0535820:intron 35.0%
! TTATCGATTGAGTACCATGA+TGG + chr4_1:12444757-12444776 None:intergenic 35.0%
CATGAAAAAACATGACGTTG+CGG - chr4_1:12444839-12444858 Msa0535820:intron 35.0%
TGAATTAACCCATACTCGAA+TGG + chr4_1:12445011-12445030 None:intergenic 35.0%
TATAGTCCGCTATATGACAA+CGG + chr4_1:12445082-12445101 None:intergenic 35.0%
!! TCCACTTTTCTAAGCAATGT+GGG + chr4_1:12445133-12445152 None:intergenic 35.0%
CCTTAAGACATTGGGTTTAT+TGG + chr4_1:12445181-12445200 None:intergenic 35.0%
CCAATAAACCCAATGTCTTA+AGG - chr4_1:12445178-12445197 Msa0535820:intron 35.0%
!!! TAAGGTTTTGAGTTGAACTG+TGG - chr4_1:12445196-12445215 Msa0535820:intron 35.0%
ATGATTCACTATTAGTGCTG+CGG - chr4_1:12445330-12445349 Msa0535820:intron 35.0%
! ATGTCAAACAGTCGTCATTT+TGG - chr4_1:12445356-12445375 Msa0535820:intron 35.0%
GCATTGTTCACATTCATCTA+AGG + chr4_1:12445397-12445416 None:intergenic 35.0%
GCTACCATGTTATAGTATTG+CGG - chr4_1:12445726-12445745 Msa0535820:intron 35.0%
AACTCCGCAATACTATAACA+TGG + chr4_1:12445733-12445752 None:intergenic 35.0%
ACACTATGCAATAGTGCTAT+AGG + chr4_1:12445826-12445845 None:intergenic 35.0%
ACTATTGCATAGTGTTGTAG+CGG - chr4_1:12445831-12445850 Msa0535820:intron 35.0%
TGATCACATGGCATTTACTA+TGG - chr4_1:12445983-12446002 Msa0535820:intron 35.0%
TGGTTACTTCACTCTTTCTT+TGG - chr4_1:12446102-12446121 Msa0535820:intron 35.0%
TCCAACAAAATTGCAGTCTT+AGG - chr4_1:12446189-12446208 Msa0535820:intron 35.0%
! ACCTAAGACTGCAATTTTGT+TGG + chr4_1:12446193-12446212 None:intergenic 35.0%
! AGCTGGAATTTCTAAAGGTA+AGG - chr4_1:12446512-12446531 Msa0535820:intron 35.0%
AACAGCTTCAAACTCGTGAT+AGG - chr4_1:12443735-12443754 Msa0535820:three_prime_UTR 40.0%
TGATGTGCTGAAAATGGATG+AGG - chr4_1:12443833-12443852 Msa0535820:three_prime_UTR 40.0%
TTAAGCATTATGGGCTCGTT+TGG - chr4_1:12443950-12443969 Msa0535820:CDS 40.0%
TAGACACCAACACATGTTGT+TGG - chr4_1:12444448-12444467 Msa0535820:intron 40.0%
TATCCTTCACAAATTGGCCA+CGG + chr4_1:12444707-12444726 None:intergenic 40.0%
! GTATTTCTGCCTTTCCATCA+TGG - chr4_1:12444740-12444759 Msa0535820:intron 40.0%
! GATTGAGTACCATGATGGAA+AGG + chr4_1:12444752-12444771 None:intergenic 40.0%
! ACACTGATTACAGAGAGTCA+TGG - chr4_1:12444776-12444795 Msa0535820:intron 40.0%
! TTATGATGGAACCCGAATCA+CGG - chr4_1:12444807-12444826 Msa0535820:intron 40.0%
ATGTCTCTATGACCGTGATT+CGG + chr4_1:12444822-12444841 None:intergenic 40.0%
ATGACGTTGCGGCTAAAATT+GGG - chr4_1:12444850-12444869 Msa0535820:intron 40.0%
AGTAAATGGCCATTCGAGTA+TGG - chr4_1:12444999-12445018 Msa0535820:intron 40.0%
GTAAATGGCCATTCGAGTAT+GGG - chr4_1:12445000-12445019 Msa0535820:intron 40.0%
TGACAACGGTCCATTTGATA+TGG + chr4_1:12445068-12445087 None:intergenic 40.0%
AATGGACCGTTGTCATATAG+CGG - chr4_1:12445073-12445092 Msa0535820:intron 40.0%
TCCCACATTGCTTAGAAAAG+TGG - chr4_1:12445129-12445148 Msa0535820:intron 40.0%
!! CTCCACTTTTCTAAGCAATG+TGG + chr4_1:12445134-12445153 None:intergenic 40.0%
CACATTGCTTAGAAAAGTGG+AGG - chr4_1:12445132-12445151 Msa0535820:intron 40.0%
! AGTATTTTCCAGCACATCTG+AGG + chr4_1:12446304-12446323 None:intergenic 40.0%
GATGTGCTGGAAAATACTTG+AGG - chr4_1:12446306-12446325 Msa0535820:intron 40.0%
! TTGAGGTTTTCATCGCAAGT+GGG - chr4_1:12446323-12446342 Msa0535820:intron 40.0%
AACAGCTTCAAACTCGTGAT+AGG - chr4_1:12443735-12443754 Msa0535820:three_prime_UTR 40.0%
TGATGTGCTGAAAATGGATG+AGG - chr4_1:12443833-12443852 Msa0535820:three_prime_UTR 40.0%
TTAAGCATTATGGGCTCGTT+TGG - chr4_1:12443950-12443969 Msa0535820:CDS 40.0%
TAGACACCAACACATGTTGT+TGG - chr4_1:12444448-12444467 Msa0535820:intron 40.0%
TATCCTTCACAAATTGGCCA+CGG + chr4_1:12444707-12444726 None:intergenic 40.0%
! GTATTTCTGCCTTTCCATCA+TGG - chr4_1:12444740-12444759 Msa0535820:intron 40.0%
! GATTGAGTACCATGATGGAA+AGG + chr4_1:12444752-12444771 None:intergenic 40.0%
! ACACTGATTACAGAGAGTCA+TGG - chr4_1:12444776-12444795 Msa0535820:intron 40.0%
! TTATGATGGAACCCGAATCA+CGG - chr4_1:12444807-12444826 Msa0535820:intron 40.0%
ATGTCTCTATGACCGTGATT+CGG + chr4_1:12444822-12444841 None:intergenic 40.0%
ATGACGTTGCGGCTAAAATT+GGG - chr4_1:12444850-12444869 Msa0535820:intron 40.0%
AGTAAATGGCCATTCGAGTA+TGG - chr4_1:12444999-12445018 Msa0535820:intron 40.0%
GTAAATGGCCATTCGAGTAT+GGG - chr4_1:12445000-12445019 Msa0535820:intron 40.0%
TGACAACGGTCCATTTGATA+TGG + chr4_1:12445068-12445087 None:intergenic 40.0%
AATGGACCGTTGTCATATAG+CGG - chr4_1:12445073-12445092 Msa0535820:intron 40.0%
TCCCACATTGCTTAGAAAAG+TGG - chr4_1:12445129-12445148 Msa0535820:intron 40.0%
!! CTCCACTTTTCTAAGCAATG+TGG + chr4_1:12445134-12445153 None:intergenic 40.0%
CACATTGCTTAGAAAAGTGG+AGG - chr4_1:12445132-12445151 Msa0535820:intron 40.0%
! AGTATTTTCCAGCACATCTG+AGG + chr4_1:12446304-12446323 None:intergenic 40.0%
GATGTGCTGGAAAATACTTG+AGG - chr4_1:12446306-12446325 Msa0535820:intron 40.0%
! TTGAGGTTTTCATCGCAAGT+GGG - chr4_1:12446323-12446342 Msa0535820:intron 40.0%
CGACATAGACACATCGACAT+TGG - chr4_1:12444362-12444381 Msa0535820:intron 45.0%
! CATGTGTTGGTGTCTAACAC+TGG + chr4_1:12444444-12444463 None:intergenic 45.0%
CCAACACATGTTGTTGGACA+CGG - chr4_1:12444454-12444473 Msa0535820:intron 45.0%
CAACACATGTTGTTGGACAC+GGG - chr4_1:12444455-12444474 Msa0535820:intron 45.0%
! CTGACACTTTTGATGGAAGG+CGG + chr4_1:12444486-12444505 None:intergenic 45.0%
!! GCACTGACACTTTTGATGGA+AGG + chr4_1:12444489-12444508 None:intergenic 45.0%
!! CAGAATTTTGACAACCACCG+TGG - chr4_1:12444687-12444706 Msa0535820:intron 45.0%
GGCCAATTTGTGAAGGATAG+CGG - chr4_1:12444708-12444727 Msa0535820:intron 45.0%
GGAAAGGCAGAAATACACCA+TGG + chr4_1:12444736-12444755 None:intergenic 45.0%
TGTCTCTATGACCGTGATTC+GGG + chr4_1:12444821-12444840 None:intergenic 45.0%
CATGACGTTGCGGCTAAAAT+TGG - chr4_1:12444849-12444868 Msa0535820:intron 45.0%
TGACGTTGCGGCTAAAATTG+GGG - chr4_1:12444851-12444870 Msa0535820:intron 45.0%
TGGTGTCCAACTCACTTGTA+TGG - chr4_1:12445216-12445235 Msa0535820:intron 45.0%
! GTTATAGTGTTGGGTCACGA+GGG + chr4_1:12445278-12445297 None:intergenic 45.0%
! TGTTATAGTGTTGGGTCACG+AGG + chr4_1:12445279-12445298 None:intergenic 45.0%
! CTTGAGGTTTTCATCGCAAG+TGG - chr4_1:12446322-12446341 Msa0535820:intron 45.0%
TGTCTGTTGTAGATGCTCCT+TGG + chr4_1:12446370-12446389 None:intergenic 45.0%
CGACATAGACACATCGACAT+TGG - chr4_1:12444362-12444381 Msa0535820:intron 45.0%
! CATGTGTTGGTGTCTAACAC+TGG + chr4_1:12444444-12444463 None:intergenic 45.0%
CCAACACATGTTGTTGGACA+CGG - chr4_1:12444454-12444473 Msa0535820:intron 45.0%
CAACACATGTTGTTGGACAC+GGG - chr4_1:12444455-12444474 Msa0535820:intron 45.0%
! CTGACACTTTTGATGGAAGG+CGG + chr4_1:12444486-12444505 None:intergenic 45.0%
!! GCACTGACACTTTTGATGGA+AGG + chr4_1:12444489-12444508 None:intergenic 45.0%
!! CAGAATTTTGACAACCACCG+TGG - chr4_1:12444687-12444706 Msa0535820:intron 45.0%
GGCCAATTTGTGAAGGATAG+CGG - chr4_1:12444708-12444727 Msa0535820:intron 45.0%
GGAAAGGCAGAAATACACCA+TGG + chr4_1:12444736-12444755 None:intergenic 45.0%
TGTCTCTATGACCGTGATTC+GGG + chr4_1:12444821-12444840 None:intergenic 45.0%
CATGACGTTGCGGCTAAAAT+TGG - chr4_1:12444849-12444868 Msa0535820:intron 45.0%
TGACGTTGCGGCTAAAATTG+GGG - chr4_1:12444851-12444870 Msa0535820:intron 45.0%
TGGTGTCCAACTCACTTGTA+TGG - chr4_1:12445216-12445235 Msa0535820:intron 45.0%
! GTTATAGTGTTGGGTCACGA+GGG + chr4_1:12445278-12445297 None:intergenic 45.0%
! TGTTATAGTGTTGGGTCACG+AGG + chr4_1:12445279-12445298 None:intergenic 45.0%
! CTTGAGGTTTTCATCGCAAG+TGG - chr4_1:12446322-12446341 Msa0535820:intron 45.0%
TGTCTGTTGTAGATGCTCCT+TGG + chr4_1:12446370-12446389 None:intergenic 45.0%
CTTAAAGGGCCCGTTTGGAA+TGG + chr4_1:12444139-12444158 None:intergenic 50.0%
CATTCCAAACGGGCCCTTTA+AGG - chr4_1:12444137-12444156 Msa0535820:intron 50.0%
AAGTCCTTAAAGGGCCCGTT+TGG + chr4_1:12444144-12444163 None:intergenic 50.0%
CCGTGTCCAACAACATGTGT+TGG + chr4_1:12444457-12444476 None:intergenic 50.0%
TCTGCCACCAAAATCCACCA+TGG + chr4_1:12444671-12444690 None:intergenic 50.0%
CGCCGCTATCCTTCACAAAT+TGG + chr4_1:12444713-12444732 None:intergenic 50.0%
GAGCAGCCATACAAGTGAGT+TGG + chr4_1:12445225-12445244 None:intergenic 50.0%
CTCACTTGTATGGCTGCTCT+TGG - chr4_1:12445226-12445245 Msa0535820:intron 50.0%
CCTAGATGATCGCCCACATT+GGG + chr4_1:12445252-12445271 None:intergenic 50.0%
GAGTTCGTTGAACAAGCCAG+AGG - chr4_1:12446268-12446287 Msa0535820:intron 50.0%
CTGAGGACACATGTTTCCTC+TGG + chr4_1:12446287-12446306 None:intergenic 50.0%
ACATGTGTCCTCAGATGTGC+TGG - chr4_1:12446293-12446312 Msa0535820:intron 50.0%
CTTAAAGGGCCCGTTTGGAA+TGG + chr4_1:12444139-12444158 None:intergenic 50.0%
CATTCCAAACGGGCCCTTTA+AGG - chr4_1:12444137-12444156 Msa0535820:intron 50.0%
AAGTCCTTAAAGGGCCCGTT+TGG + chr4_1:12444144-12444163 None:intergenic 50.0%
CCGTGTCCAACAACATGTGT+TGG + chr4_1:12444457-12444476 None:intergenic 50.0%
TCTGCCACCAAAATCCACCA+TGG + chr4_1:12444671-12444690 None:intergenic 50.0%
CGCCGCTATCCTTCACAAAT+TGG + chr4_1:12444713-12444732 None:intergenic 50.0%
GAGCAGCCATACAAGTGAGT+TGG + chr4_1:12445225-12445244 None:intergenic 50.0%
CTCACTTGTATGGCTGCTCT+TGG - chr4_1:12445226-12445245 Msa0535820:intron 50.0%
CCTAGATGATCGCCCACATT+GGG + chr4_1:12445252-12445271 None:intergenic 50.0%
GAGTTCGTTGAACAAGCCAG+AGG - chr4_1:12446268-12446287 Msa0535820:intron 50.0%
CTGAGGACACATGTTTCCTC+TGG + chr4_1:12446287-12446306 None:intergenic 50.0%
ACATGTGTCCTCAGATGTGC+TGG - chr4_1:12446293-12446312 Msa0535820:intron 50.0%
TATCCACCATGGCCGAACCA+TGG - chr4_1:12444639-12444658 Msa0535820:intron 55.0%
!!! GTGTCGGCCATGGTGGATTT+TGG - chr4_1:12444661-12444680 Msa0535820:intron 55.0%
!!! TCGGCCATGGTGGATTTTGG+TGG - chr4_1:12444664-12444683 Msa0535820:intron 55.0%
CCTTCACAAATTGGCCACGG+TGG + chr4_1:12444704-12444723 None:intergenic 55.0%
CCACCGTGGCCAATTTGTGA+AGG - chr4_1:12444701-12444720 Msa0535820:intron 55.0%
CCCAATGTGGGCGATCATCT+AGG - chr4_1:12445249-12445268 Msa0535820:intron 55.0%
GCCTAGATGATCGCCCACAT+TGG + chr4_1:12445253-12445272 None:intergenic 55.0%
TATCCACCATGGCCGAACCA+TGG - chr4_1:12444639-12444658 Msa0535820:intron 55.0%
!!! GTGTCGGCCATGGTGGATTT+TGG - chr4_1:12444661-12444680 Msa0535820:intron 55.0%
!!! TCGGCCATGGTGGATTTTGG+TGG - chr4_1:12444664-12444683 Msa0535820:intron 55.0%
CCTTCACAAATTGGCCACGG+TGG + chr4_1:12444704-12444723 None:intergenic 55.0%
CCACCGTGGCCAATTTGTGA+AGG - chr4_1:12444701-12444720 Msa0535820:intron 55.0%
CCCAATGTGGGCGATCATCT+AGG - chr4_1:12445249-12445268 Msa0535820:intron 55.0%
GCCTAGATGATCGCCCACAT+TGG + chr4_1:12445253-12445272 None:intergenic 55.0%
! ACACCATGGTTCGGCCATGG+TGG + chr4_1:12444645-12444664 None:intergenic 60.0%
CCATGGCCGAACCATGGTGT+CGG - chr4_1:12444645-12444664 Msa0535820:intron 60.0%
CCATGGCCGACACCATGGTT+CGG + chr4_1:12444654-12444673 None:intergenic 60.0%
!! AACCATGGTGTCGGCCATGG+TGG - chr4_1:12444654-12444673 Msa0535820:intron 60.0%
ATCCACCATGGCCGACACCA+TGG + chr4_1:12444659-12444678 None:intergenic 60.0%
! TGTGAAGGATAGCGGCGCCA+TGG - chr4_1:12444716-12444735 Msa0535820:intron 60.0%
TGGCTGCTCTTGGCCCAATG+TGG - chr4_1:12445236-12445255 Msa0535820:intron 60.0%
GGCTGCTCTTGGCCCAATGT+GGG - chr4_1:12445237-12445256 Msa0535820:intron 60.0%
! ACACCATGGTTCGGCCATGG+TGG + chr4_1:12444645-12444664 None:intergenic 60.0%
CCATGGCCGAACCATGGTGT+CGG - chr4_1:12444645-12444664 Msa0535820:intron 60.0%
CCATGGCCGACACCATGGTT+CGG + chr4_1:12444654-12444673 None:intergenic 60.0%
!! AACCATGGTGTCGGCCATGG+TGG - chr4_1:12444654-12444673 Msa0535820:intron 60.0%
ATCCACCATGGCCGACACCA+TGG + chr4_1:12444659-12444678 None:intergenic 60.0%
! TGTGAAGGATAGCGGCGCCA+TGG - chr4_1:12444716-12444735 Msa0535820:intron 60.0%
TGGCTGCTCTTGGCCCAATG+TGG - chr4_1:12445236-12445255 Msa0535820:intron 60.0%
GGCTGCTCTTGGCCCAATGT+GGG - chr4_1:12445237-12445256 Msa0535820:intron 60.0%
! CCGACACCATGGTTCGGCCA+TGG + chr4_1:12444648-12444667 None:intergenic 65.0%
!! CCGAACCATGGTGTCGGCCA+TGG - chr4_1:12444651-12444670 Msa0535820:intron 65.0%
! CCGACACCATGGTTCGGCCA+TGG + chr4_1:12444648-12444667 None:intergenic 65.0%
!! CCGAACCATGGTGTCGGCCA+TGG - chr4_1:12444651-12444670 Msa0535820:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 12443618 12446665 12443618 ID=Msa0535820;Name=Msa0535820
chr4_1 mRNA 12443618 12446665 12443618 ID=Msa0535820-mRNA-1;Parent=Msa0535820;Name=Msa0535820-mRNA-1;_AED=0.33;_eAED=0.33;_QI=44|1|1|1|1|1|2|268|95
chr4_1 exon 12443618 12444078 12443618 ID=Msa0535820-mRNA-1:exon:4004;Parent=Msa0535820-mRNA-1
chr4_1 exon 12446527 12446665 12446527 ID=Msa0535820-mRNA-1:exon:4003;Parent=Msa0535820-mRNA-1
chr4_1 five_prime_UTR 12446622 12446665 12446622 ID=Msa0535820-mRNA-1:five_prime_utr;Parent=Msa0535820-mRNA-1
chr4_1 CDS 12446527 12446621 12446527 ID=Msa0535820-mRNA-1:cds;Parent=Msa0535820-mRNA-1
chr4_1 CDS 12443886 12444078 12443886 ID=Msa0535820-mRNA-1:cds;Parent=Msa0535820-mRNA-1
chr4_1 three_prime_UTR 12443618 12443885 12443618 ID=Msa0535820-mRNA-1:three_prime_utr;Parent=Msa0535820-mRNA-1
Gene Sequence

>Msa0535820

ATGGATAAGAACATTTTTGGAGAAGTAGATAATCTTCCTGAAGAAGATAAGAAAAGAATGACTACTATGGTTGAACAGCTTCAAACTCGTGATAGTCTTAGGTTGTATAATTCATTAGTTGAGAGATGTTTTAACAATTGCGTCAATTCATTCTATCGGAGTTCGTTGAACAAGCCAGAGGAAACATGTGTCCTCAGATGTGCTGGAAAATACTTGAGGTTTTCATCGCAAGTGGGCGTTAAATTTTCAGATATCAACCAAGGAGCATCTACAACAGACAAACAATAA
Protein sequence

>Msa0535820

MDKNIFGEVDNLPEEDKKRMTTMVEQLQTRDSLRLYNSLVERCFNNCVNSFYRSSLNKPEETCVLRCAGKYLRFSSQVGVKFSDINQGASTTDKQ*