AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0542860

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0542860 MtrunA17_Chr4g0022591 96.667 30 1 0 1 30 1 30 8.86e-15 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 35 sgRNAs with CRISPR-Local

Find 292 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATAATCATCAAAATATATT+TGG 0.160267 4_1:+28248694 Msa0542860:three_prime_UTR
GAAATGGAGAAATAAAATTT+TGG 0.187748 4_1:+28248473 Msa0542860:exon
CAAAATATATTTGGATTTAA+AGG 0.194605 4_1:+28248703 Msa0542860:three_prime_UTR
ACAATCAAACAACTCCTAAT+TGG 0.215802 4_1:-28246636 None:intergenic
AGTTGTTTGATTGTTAAATT+TGG 0.222497 4_1:+28246645 Msa0542860:five_prime_UTR
TTCTCTGGATTATGACTTCT+TGG 0.237305 4_1:+28248582 Msa0542860:three_prime_UTR
TTCTGCTTCTTTGTACTATC+AGG 0.282569 4_1:+28246741 Msa0542860:CDS
GTTTAGACTTAGATCCAATT+AGG 0.286477 4_1:+28246622 Msa0542860:five_prime_UTR
AATATGTGGAGTCGGTTAAA+TGG 0.305259 4_1:-28248751 None:intergenic
ATTTAACCGACTCCACATAT+TGG 0.326024 4_1:+28248753 Msa0542860:three_prime_UTR
GATCGATGCTGCAGAGAAAA+AGG 0.327450 4_1:+28248513 Msa0542860:three_prime_UTR
TTTAACCGACTCCACATATT+GGG 0.357293 4_1:+28248754 Msa0542860:three_prime_UTR
TTTCAATACCCCTGGTGTTA+AGG 0.376934 4_1:+28248633 Msa0542860:three_prime_UTR
GTTGTTTGATTGTTAAATTT+GGG 0.447186 4_1:+28246646 Msa0542860:five_prime_UTR
AGCAGAAAGTTGGAAGAGAA+AGG 0.447754 4_1:-28246725 None:intergenic
CCTTCTTCAACAAAGCCTCC+AGG 0.456795 4_1:+28246687 Msa0542860:CDS
GTCTAATATTGAAAACTTAG+TGG 0.466929 4_1:+28248546 Msa0542860:three_prime_UTR
CCTGGAGGCTTTGTTGAAGA+AGG 0.475102 4_1:-28246687 None:intergenic
AGGGTGAATGGTGAGGGCCC+TGG 0.480488 4_1:-28246705 None:intergenic
AGTACAAAGAAGCAGAAAGT+TGG 0.485887 4_1:-28246735 None:intergenic
GCAGAAAGTTGGAAGAGAAA+GGG 0.510279 4_1:-28246724 None:intergenic
ATTGAAAACTTAGTGGGAAG+TGG 0.521615 4_1:+28248553 Msa0542860:three_prime_UTR
TCTAATATTGAAAACTTAGT+GGG 0.522285 4_1:+28248547 Msa0542860:three_prime_UTR
GTTGGAAGAGAAAGGGTGAA+TGG 0.534449 4_1:-28246717 None:intergenic
ATTTGGGTGAATATGACGAT+TGG 0.536274 4_1:+28246662 Msa0542860:exon
CTTCTTCAACAAAGCCTCCA+GGG 0.547343 4_1:+28246688 Msa0542860:CDS
GACTTCTTGGTTGTAGCAGT+AGG 0.555062 4_1:+28248595 Msa0542860:three_prime_UTR
GTAAATACTTTCAATACCCC+TGG 0.564589 4_1:+28248625 Msa0542860:three_prime_UTR
AGAGAAAGGGTGAATGGTGA+GGG 0.590542 4_1:-28246711 None:intergenic
GTGAATGGTGAGGGCCCTGG+AGG 0.595344 4_1:-28246702 None:intergenic
TGACAGTTCTCCTTAACACC+AGG 0.601556 4_1:-28248643 None:intergenic
CATACACGCGTCAATGAATG+TGG 0.619036 4_1:+28246541 None:intergenic
AAGAGAAAGGGTGAATGGTG+AGG 0.623100 4_1:-28246712 None:intergenic
GACAGTTCTCCTTAACACCA+GGG 0.658869 4_1:-28248642 None:intergenic
ACAGTTCTCCTTAACACCAG+GGG 0.714844 4_1:-28248641 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTCTATTTATAGTATTTT+AGG - chr4_1:28246804-28246823 None:intergenic 10.0%
!! ATTTATATATAACACATTAA+TGG + chr4_1:28247112-28247131 Msa0542860:intron 10.0%
!!! ATTTCTATTTATAGTATTTT+AGG - chr4_1:28246804-28246823 None:intergenic 10.0%
!! ATTTATATATAACACATTAA+TGG + chr4_1:28247112-28247131 Msa0542860:intron 10.0%
!! ATTAATTTATATTTATGTCG+AGG - chr4_1:28247174-28247193 None:intergenic 15.0%
!! TGATATAACAAATTTATATG+TGG - chr4_1:28247487-28247506 None:intergenic 15.0%
!! CTAAAATAAAATAGCAATAT+GGG - chr4_1:28247532-28247551 None:intergenic 15.0%
!! ACTAAAATAAAATAGCAATA+TGG - chr4_1:28247533-28247552 None:intergenic 15.0%
!!! TGCTATTTTATTTTAGTAAT+TGG + chr4_1:28247536-28247555 Msa0542860:intron 15.0%
!!! ATCAATGCAATTTTTTTTTT+TGG - chr4_1:28248210-28248229 None:intergenic 15.0%
!!! TGTCTAATCATATTTTTTTT+TGG + chr4_1:28248392-28248411 Msa0542860:intron 15.0%
!! ATTGTCTAATCATTTAATAA+TGG + chr4_1:28248422-28248441 Msa0542860:intron 15.0%
!!! AAATGGAGAAATAAAATTTT+GGG + chr4_1:28248474-28248493 Msa0542860:exon 15.0%
!! CATAATCATCAAAATATATT+TGG + chr4_1:28248694-28248713 Msa0542860:three_prime_UTR 15.0%
!! CAAAATATATTTGGATTTAA+AGG + chr4_1:28248703-28248722 Msa0542860:three_prime_UTR 15.0%
!! ATTAATTTATATTTATGTCG+AGG - chr4_1:28247174-28247193 None:intergenic 15.0%
!! TGATATAACAAATTTATATG+TGG - chr4_1:28247487-28247506 None:intergenic 15.0%
!! CTAAAATAAAATAGCAATAT+GGG - chr4_1:28247532-28247551 None:intergenic 15.0%
!! ACTAAAATAAAATAGCAATA+TGG - chr4_1:28247533-28247552 None:intergenic 15.0%
!!! TGCTATTTTATTTTAGTAAT+TGG + chr4_1:28247536-28247555 Msa0542860:intron 15.0%
!!! ATCAATGCAATTTTTTTTTT+TGG - chr4_1:28248210-28248229 None:intergenic 15.0%
!!! TGTCTAATCATATTTTTTTT+TGG + chr4_1:28248392-28248411 Msa0542860:intron 15.0%
!! ATTGTCTAATCATTTAATAA+TGG + chr4_1:28248422-28248441 Msa0542860:intron 15.0%
!!! AAATGGAGAAATAAAATTTT+GGG + chr4_1:28248474-28248493 Msa0542860:exon 15.0%
!! CATAATCATCAAAATATATT+TGG + chr4_1:28248694-28248713 Msa0542860:three_prime_UTR 15.0%
!! CAAAATATATTTGGATTTAA+AGG + chr4_1:28248703-28248722 Msa0542860:three_prime_UTR 15.0%
!! AGTTGTTTGATTGTTAAATT+TGG + chr4_1:28246645-28246664 Msa0542860:five_prime_UTR 20.0%
!! GTTGTTTGATTGTTAAATTT+GGG + chr4_1:28246646-28246665 Msa0542860:five_prime_UTR 20.0%
!!! ATTTATAGTATTTTAGGACA+AGG - chr4_1:28246798-28246817 None:intergenic 20.0%
!! AGAAATTTCAAAACAAGTAA+TGG - chr4_1:28246861-28246880 None:intergenic 20.0%
!! TGCAATTAAATCAGTTTAAT+TGG + chr4_1:28247059-28247078 Msa0542860:intron 20.0%
!!! TTATATCTGAAAGTTTTATC+TGG + chr4_1:28248344-28248363 Msa0542860:intron 20.0%
!!! GAAATGGAGAAATAAAATTT+TGG + chr4_1:28248473-28248492 Msa0542860:exon 20.0%
!! TCTAATATTGAAAACTTAGT+GGG + chr4_1:28248547-28248566 Msa0542860:three_prime_UTR 20.0%
!! AGTTGTTTGATTGTTAAATT+TGG + chr4_1:28246645-28246664 Msa0542860:five_prime_UTR 20.0%
!! GTTGTTTGATTGTTAAATTT+GGG + chr4_1:28246646-28246665 Msa0542860:five_prime_UTR 20.0%
!!! ATTTATAGTATTTTAGGACA+AGG - chr4_1:28246798-28246817 None:intergenic 20.0%
!! AGAAATTTCAAAACAAGTAA+TGG - chr4_1:28246861-28246880 None:intergenic 20.0%
!! TGCAATTAAATCAGTTTAAT+TGG + chr4_1:28247059-28247078 Msa0542860:intron 20.0%
!!! TTATATCTGAAAGTTTTATC+TGG + chr4_1:28248344-28248363 Msa0542860:intron 20.0%
!!! GAAATGGAGAAATAAAATTT+TGG + chr4_1:28248473-28248492 Msa0542860:exon 20.0%
!! TCTAATATTGAAAACTTAGT+GGG + chr4_1:28248547-28248566 Msa0542860:three_prime_UTR 20.0%
!! CAGTTTAATTGGATATTTTG+TGG + chr4_1:28247070-28247089 Msa0542860:intron 25.0%
!! TAACACATTAATGGATCAAT+TGG + chr4_1:28247121-28247140 Msa0542860:intron 25.0%
! TTAACACTCATTTCCTTATT+GGG - chr4_1:28247311-28247330 None:intergenic 25.0%
! TTTAACACTCATTTCCTTAT+TGG - chr4_1:28247312-28247331 None:intergenic 25.0%
! AACAAATTTATATGTGGCAA+TGG - chr4_1:28247481-28247500 None:intergenic 25.0%
!!! TTTTTTCCACTTAGAAACAT+TGG + chr4_1:28247582-28247601 Msa0542860:intron 25.0%
!! TTTTTCCACTTAGAAACATT+GGG + chr4_1:28247583-28247602 Msa0542860:intron 25.0%
! CTAATAAATATGATCGTTAC+AGG + chr4_1:28248283-28248302 Msa0542860:intron 25.0%
! AACTTTCAGATATAATCACA+AGG - chr4_1:28248339-28248358 None:intergenic 25.0%
! GTCTAATATTGAAAACTTAG+TGG + chr4_1:28248546-28248565 Msa0542860:three_prime_UTR 25.0%
!! CAGTTTAATTGGATATTTTG+TGG + chr4_1:28247070-28247089 Msa0542860:intron 25.0%
!! TAACACATTAATGGATCAAT+TGG + chr4_1:28247121-28247140 Msa0542860:intron 25.0%
! TTAACACTCATTTCCTTATT+GGG - chr4_1:28247311-28247330 None:intergenic 25.0%
! TTTAACACTCATTTCCTTAT+TGG - chr4_1:28247312-28247331 None:intergenic 25.0%
! AACAAATTTATATGTGGCAA+TGG - chr4_1:28247481-28247500 None:intergenic 25.0%
!!! TTTTTTCCACTTAGAAACAT+TGG + chr4_1:28247582-28247601 Msa0542860:intron 25.0%
!! TTTTTCCACTTAGAAACATT+GGG + chr4_1:28247583-28247602 Msa0542860:intron 25.0%
! CTAATAAATATGATCGTTAC+AGG + chr4_1:28248283-28248302 Msa0542860:intron 25.0%
! AACTTTCAGATATAATCACA+AGG - chr4_1:28248339-28248358 None:intergenic 25.0%
! GTCTAATATTGAAAACTTAG+TGG + chr4_1:28248546-28248565 Msa0542860:three_prime_UTR 25.0%
GTTTAGACTTAGATCCAATT+AGG + chr4_1:28246622-28246641 Msa0542860:five_prime_UTR 30.0%
ACAATCAAACAACTCCTAAT+TGG - chr4_1:28246639-28246658 None:intergenic 30.0%
ATAGAAATAGAACATGCAAC+TGG + chr4_1:28246816-28246835 Msa0542860:intron 30.0%
! CAAAACAAGTAATGGAGATT+TGG - chr4_1:28246853-28246872 None:intergenic 30.0%
CAATAGATTGAAATGATCTG+TGG + chr4_1:28246962-28246981 Msa0542860:intron 30.0%
TATATTTATGTCGAGGTTGA+GGG - chr4_1:28247167-28247186 None:intergenic 30.0%
TTATATTTATGTCGAGGTTG+AGG - chr4_1:28247168-28247187 None:intergenic 30.0%
TTATTGAGTGAAATACATGC+AGG + chr4_1:28247229-28247248 Msa0542860:intron 30.0%
TTATTGGGTGAAATACATAC+TGG - chr4_1:28247296-28247315 None:intergenic 30.0%
AGTATGTATTTCACCCAATA+AGG + chr4_1:28247295-28247314 Msa0542860:intron 30.0%
ATTAAATACACCATAAGCAC+AGG - chr4_1:28247668-28247687 None:intergenic 30.0%
! TTTTTAATGATGTTTCGGAC+CGG - chr4_1:28247997-28248016 None:intergenic 30.0%
! CCACCTTTTTAATGATGTTT+CGG - chr4_1:28248002-28248021 None:intergenic 30.0%
TTCCCTAGTTATTCATCTTA+AGG + chr4_1:28248155-28248174 Msa0542860:intron 30.0%
TCCCTAGTTATTCATCTTAA+GGG + chr4_1:28248156-28248175 Msa0542860:intron 30.0%
! TGGAGAAATAAAATTTTGGG+AGG + chr4_1:28248477-28248496 Msa0542860:exon 30.0%
!! TAAAGCTTTTTCCCAATATG+TGG - chr4_1:28248768-28248787 None:intergenic 30.0%
GTTTAGACTTAGATCCAATT+AGG + chr4_1:28246622-28246641 Msa0542860:five_prime_UTR 30.0%
ACAATCAAACAACTCCTAAT+TGG - chr4_1:28246639-28246658 None:intergenic 30.0%
ATAGAAATAGAACATGCAAC+TGG + chr4_1:28246816-28246835 Msa0542860:intron 30.0%
! CAAAACAAGTAATGGAGATT+TGG - chr4_1:28246853-28246872 None:intergenic 30.0%
CAATAGATTGAAATGATCTG+TGG + chr4_1:28246962-28246981 Msa0542860:intron 30.0%
TATATTTATGTCGAGGTTGA+GGG - chr4_1:28247167-28247186 None:intergenic 30.0%
TTATATTTATGTCGAGGTTG+AGG - chr4_1:28247168-28247187 None:intergenic 30.0%
TTATTGAGTGAAATACATGC+AGG + chr4_1:28247229-28247248 Msa0542860:intron 30.0%
TTATTGGGTGAAATACATAC+TGG - chr4_1:28247296-28247315 None:intergenic 30.0%
AGTATGTATTTCACCCAATA+AGG + chr4_1:28247295-28247314 Msa0542860:intron 30.0%
ATTAAATACACCATAAGCAC+AGG - chr4_1:28247668-28247687 None:intergenic 30.0%
! TTTTTAATGATGTTTCGGAC+CGG - chr4_1:28247997-28248016 None:intergenic 30.0%
! CCACCTTTTTAATGATGTTT+CGG - chr4_1:28248002-28248021 None:intergenic 30.0%
TTCCCTAGTTATTCATCTTA+AGG + chr4_1:28248155-28248174 Msa0542860:intron 30.0%
TCCCTAGTTATTCATCTTAA+GGG + chr4_1:28248156-28248175 Msa0542860:intron 30.0%
! TGGAGAAATAAAATTTTGGG+AGG + chr4_1:28248477-28248496 Msa0542860:exon 30.0%
!! TAAAGCTTTTTCCCAATATG+TGG - chr4_1:28248768-28248787 None:intergenic 30.0%
ATTTGGGTGAATATGACGAT+TGG + chr4_1:28246662-28246681 Msa0542860:exon 35.0%
AGTACAAAGAAGCAGAAAGT+TGG - chr4_1:28246738-28246757 None:intergenic 35.0%
TTCTGCTTCTTTGTACTATC+AGG + chr4_1:28246741-28246760 Msa0542860:CDS 35.0%
! ATTGCAGCAAAAGTTGTTTC+AGG - chr4_1:28247045-28247064 None:intergenic 35.0%
TGAATGAGTTCCACATAAAG+TGG + chr4_1:28247265-28247284 Msa0542860:intron 35.0%
GTAATTGGATGACAGACAAA+AGG + chr4_1:28247551-28247570 Msa0542860:intron 35.0%
! AATCTCCCAATGTTTCTAAG+TGG - chr4_1:28247591-28247610 None:intergenic 35.0%
ATTAAGCTTTCCTGTGCTTA+TGG + chr4_1:28247655-28247674 Msa0542860:intron 35.0%
TCATTGAATCTACTTGCTCT+TGG + chr4_1:28247715-28247734 Msa0542860:intron 35.0%
CATTGAATCTACTTGCTCTT+GGG + chr4_1:28247716-28247735 Msa0542860:intron 35.0%
GGGTGATGATCTTCAATATT+AGG - chr4_1:28247799-28247818 None:intergenic 35.0%
GTAAATGCAAAGTAGTTACG+AGG - chr4_1:28247919-28247938 None:intergenic 35.0%
GGTCCGAAACATCATTAAAA+AGG + chr4_1:28247996-28248015 Msa0542860:intron 35.0%
CCGAAACATCATTAAAAAGG+TGG + chr4_1:28247999-28248018 Msa0542860:intron 35.0%
TAGGGACATTGCAATATACA+GGG + chr4_1:28248112-28248131 Msa0542860:intron 35.0%
CCCCTTAAGATGAATAACTA+GGG - chr4_1:28248160-28248179 None:intergenic 35.0%
CCCTAGTTATTCATCTTAAG+GGG + chr4_1:28248157-28248176 Msa0542860:intron 35.0%
ACCCCTTAAGATGAATAACT+AGG - chr4_1:28248161-28248180 None:intergenic 35.0%
!!! TTTTTGGTTAAGAAGCCTAG+TGG - chr4_1:28248194-28248213 None:intergenic 35.0%
! ACTTTGTTTGTGTCTTGAGA+TGG + chr4_1:28248310-28248329 Msa0542860:intron 35.0%
! TTTTTCCCATTGTAGCGAAA+TGG + chr4_1:28248457-28248476 Msa0542860:intron 35.0%
TTTCTCCATTTCGCTACAAT+GGG - chr4_1:28248465-28248484 None:intergenic 35.0%
ATTTCTCCATTTCGCTACAA+TGG - chr4_1:28248466-28248485 None:intergenic 35.0%
CAGAGAAAAAGGTTGTTTGT+CGG + chr4_1:28248524-28248543 Msa0542860:three_prime_UTR 35.0%
ATTGAAAACTTAGTGGGAAG+TGG + chr4_1:28248553-28248572 Msa0542860:three_prime_UTR 35.0%
TTCTCTGGATTATGACTTCT+TGG + chr4_1:28248582-28248601 Msa0542860:three_prime_UTR 35.0%
GTAAATACTTTCAATACCCC+TGG + chr4_1:28248625-28248644 Msa0542860:three_prime_UTR 35.0%
AATATGTGGAGTCGGTTAAA+TGG - chr4_1:28248754-28248773 None:intergenic 35.0%
! ATTTAACCGACTCCACATAT+TGG + chr4_1:28248753-28248772 Msa0542860:three_prime_UTR 35.0%
! TTTAACCGACTCCACATATT+GGG + chr4_1:28248754-28248773 Msa0542860:three_prime_UTR 35.0%
! TTTTTCCCAATATGTGGAGT+CGG - chr4_1:28248762-28248781 None:intergenic 35.0%
ATTTGGGTGAATATGACGAT+TGG + chr4_1:28246662-28246681 Msa0542860:exon 35.0%
AGTACAAAGAAGCAGAAAGT+TGG - chr4_1:28246738-28246757 None:intergenic 35.0%
TTCTGCTTCTTTGTACTATC+AGG + chr4_1:28246741-28246760 Msa0542860:CDS 35.0%
! ATTGCAGCAAAAGTTGTTTC+AGG - chr4_1:28247045-28247064 None:intergenic 35.0%
TGAATGAGTTCCACATAAAG+TGG + chr4_1:28247265-28247284 Msa0542860:intron 35.0%
GTAATTGGATGACAGACAAA+AGG + chr4_1:28247551-28247570 Msa0542860:intron 35.0%
! AATCTCCCAATGTTTCTAAG+TGG - chr4_1:28247591-28247610 None:intergenic 35.0%
ATTAAGCTTTCCTGTGCTTA+TGG + chr4_1:28247655-28247674 Msa0542860:intron 35.0%
TCATTGAATCTACTTGCTCT+TGG + chr4_1:28247715-28247734 Msa0542860:intron 35.0%
CATTGAATCTACTTGCTCTT+GGG + chr4_1:28247716-28247735 Msa0542860:intron 35.0%
GGGTGATGATCTTCAATATT+AGG - chr4_1:28247799-28247818 None:intergenic 35.0%
GTAAATGCAAAGTAGTTACG+AGG - chr4_1:28247919-28247938 None:intergenic 35.0%
GGTCCGAAACATCATTAAAA+AGG + chr4_1:28247996-28248015 Msa0542860:intron 35.0%
CCGAAACATCATTAAAAAGG+TGG + chr4_1:28247999-28248018 Msa0542860:intron 35.0%
TAGGGACATTGCAATATACA+GGG + chr4_1:28248112-28248131 Msa0542860:intron 35.0%
CCCCTTAAGATGAATAACTA+GGG - chr4_1:28248160-28248179 None:intergenic 35.0%
CCCTAGTTATTCATCTTAAG+GGG + chr4_1:28248157-28248176 Msa0542860:intron 35.0%
ACCCCTTAAGATGAATAACT+AGG - chr4_1:28248161-28248180 None:intergenic 35.0%
!!! TTTTTGGTTAAGAAGCCTAG+TGG - chr4_1:28248194-28248213 None:intergenic 35.0%
! ACTTTGTTTGTGTCTTGAGA+TGG + chr4_1:28248310-28248329 Msa0542860:intron 35.0%
! TTTTTCCCATTGTAGCGAAA+TGG + chr4_1:28248457-28248476 Msa0542860:intron 35.0%
TTTCTCCATTTCGCTACAAT+GGG - chr4_1:28248465-28248484 None:intergenic 35.0%
ATTTCTCCATTTCGCTACAA+TGG - chr4_1:28248466-28248485 None:intergenic 35.0%
CAGAGAAAAAGGTTGTTTGT+CGG + chr4_1:28248524-28248543 Msa0542860:three_prime_UTR 35.0%
ATTGAAAACTTAGTGGGAAG+TGG + chr4_1:28248553-28248572 Msa0542860:three_prime_UTR 35.0%
TTCTCTGGATTATGACTTCT+TGG + chr4_1:28248582-28248601 Msa0542860:three_prime_UTR 35.0%
GTAAATACTTTCAATACCCC+TGG + chr4_1:28248625-28248644 Msa0542860:three_prime_UTR 35.0%
AATATGTGGAGTCGGTTAAA+TGG - chr4_1:28248754-28248773 None:intergenic 35.0%
! ATTTAACCGACTCCACATAT+TGG + chr4_1:28248753-28248772 Msa0542860:three_prime_UTR 35.0%
! TTTAACCGACTCCACATATT+GGG + chr4_1:28248754-28248773 Msa0542860:three_prime_UTR 35.0%
! TTTTTCCCAATATGTGGAGT+CGG - chr4_1:28248762-28248781 None:intergenic 35.0%
GCAGAAAGTTGGAAGAGAAA+GGG - chr4_1:28246727-28246746 None:intergenic 40.0%
AGCAGAAAGTTGGAAGAGAA+AGG - chr4_1:28246728-28246747 None:intergenic 40.0%
GACAAGGAAGAGAAAAGTGA+AGG - chr4_1:28246782-28246801 None:intergenic 40.0%
TCAATCTGTTCTCTTCACAG+AGG - chr4_1:28246915-28246934 None:intergenic 40.0%
TGTCACACAAATGCTATCTG+TGG + chr4_1:28247018-28247037 Msa0542860:intron 40.0%
AGAGTAACTCTAGCAACACT+CGG + chr4_1:28247202-28247221 Msa0542860:intron 40.0%
TGTGGAACTCATTCACAATG+TGG - chr4_1:28247260-28247279 None:intergenic 40.0%
ATGAGTTCCACATAAAGTGG+TGG + chr4_1:28247268-28247287 Msa0542860:intron 40.0%
TGAGTTCCACATAAAGTGGT+GGG + chr4_1:28247269-28247288 Msa0542860:intron 40.0%
!!! ACCACGACTTTCTTTTTTCG+GGG - chr4_1:28247820-28247839 None:intergenic 40.0%
!!! GACCACGACTTTCTTTTTTC+GGG - chr4_1:28247821-28247840 None:intergenic 40.0%
!!! GGACCACGACTTTCTTTTTT+CGG - chr4_1:28247822-28247841 None:intergenic 40.0%
AAAAAGAAAGTCGTGGTCCT+TGG + chr4_1:28247823-28247842 Msa0542860:intron 40.0%
TACCAGTTTCCTTAAGGGTT+TGG + chr4_1:28247861-28247880 Msa0542860:intron 40.0%
ATCCAAACCCTTAAGGAAAC+TGG - chr4_1:28247866-28247885 None:intergenic 40.0%
AGTTTCCTTAAGGGTTTGGA+TGG + chr4_1:28247865-28247884 Msa0542860:intron 40.0%
ATGAACCATCCAAACCCTTA+AGG - chr4_1:28247873-28247892 None:intergenic 40.0%
CAAGTGACACTCGGTAATAA+GGG - chr4_1:28247943-28247962 None:intergenic 40.0%
ACAAGTGACACTCGGTAATA+AGG - chr4_1:28247944-28247963 None:intergenic 40.0%
!! TTATTACCGAGTGTCACTTG+TGG + chr4_1:28247943-28247962 Msa0542860:intron 40.0%
!! TATTACCGAGTGTCACTTGT+GGG + chr4_1:28247944-28247963 Msa0542860:intron 40.0%
! TGAGCATAACTCAGTTGGTA+GGG + chr4_1:28248094-28248113 Msa0542860:intron 40.0%
GTAGGGACATTGCAATATAC+AGG + chr4_1:28248111-28248130 Msa0542860:intron 40.0%
AGGGACATTGCAATATACAG+GGG + chr4_1:28248113-28248132 Msa0542860:intron 40.0%
ATGAATAACTAGGGAATCCC+AGG - chr4_1:28248151-28248170 None:intergenic 40.0%
! TTTCAATACCCCTGGTGTTA+AGG + chr4_1:28248633-28248652 Msa0542860:three_prime_UTR 40.0%
!!! GGAGAACTGTCATTTTCTGA+AGG + chr4_1:28248654-28248673 Msa0542860:three_prime_UTR 40.0%
GCAGAAAGTTGGAAGAGAAA+GGG - chr4_1:28246727-28246746 None:intergenic 40.0%
AGCAGAAAGTTGGAAGAGAA+AGG - chr4_1:28246728-28246747 None:intergenic 40.0%
GACAAGGAAGAGAAAAGTGA+AGG - chr4_1:28246782-28246801 None:intergenic 40.0%
TCAATCTGTTCTCTTCACAG+AGG - chr4_1:28246915-28246934 None:intergenic 40.0%
TGTCACACAAATGCTATCTG+TGG + chr4_1:28247018-28247037 Msa0542860:intron 40.0%
AGAGTAACTCTAGCAACACT+CGG + chr4_1:28247202-28247221 Msa0542860:intron 40.0%
TGTGGAACTCATTCACAATG+TGG - chr4_1:28247260-28247279 None:intergenic 40.0%
ATGAGTTCCACATAAAGTGG+TGG + chr4_1:28247268-28247287 Msa0542860:intron 40.0%
TGAGTTCCACATAAAGTGGT+GGG + chr4_1:28247269-28247288 Msa0542860:intron 40.0%
!!! ACCACGACTTTCTTTTTTCG+GGG - chr4_1:28247820-28247839 None:intergenic 40.0%
!!! GACCACGACTTTCTTTTTTC+GGG - chr4_1:28247821-28247840 None:intergenic 40.0%
!!! GGACCACGACTTTCTTTTTT+CGG - chr4_1:28247822-28247841 None:intergenic 40.0%
AAAAAGAAAGTCGTGGTCCT+TGG + chr4_1:28247823-28247842 Msa0542860:intron 40.0%
TACCAGTTTCCTTAAGGGTT+TGG + chr4_1:28247861-28247880 Msa0542860:intron 40.0%
ATCCAAACCCTTAAGGAAAC+TGG - chr4_1:28247866-28247885 None:intergenic 40.0%
AGTTTCCTTAAGGGTTTGGA+TGG + chr4_1:28247865-28247884 Msa0542860:intron 40.0%
ATGAACCATCCAAACCCTTA+AGG - chr4_1:28247873-28247892 None:intergenic 40.0%
CAAGTGACACTCGGTAATAA+GGG - chr4_1:28247943-28247962 None:intergenic 40.0%
ACAAGTGACACTCGGTAATA+AGG - chr4_1:28247944-28247963 None:intergenic 40.0%
!! TTATTACCGAGTGTCACTTG+TGG + chr4_1:28247943-28247962 Msa0542860:intron 40.0%
!! TATTACCGAGTGTCACTTGT+GGG + chr4_1:28247944-28247963 Msa0542860:intron 40.0%
! TGAGCATAACTCAGTTGGTA+GGG + chr4_1:28248094-28248113 Msa0542860:intron 40.0%
GTAGGGACATTGCAATATAC+AGG + chr4_1:28248111-28248130 Msa0542860:intron 40.0%
AGGGACATTGCAATATACAG+GGG + chr4_1:28248113-28248132 Msa0542860:intron 40.0%
ATGAATAACTAGGGAATCCC+AGG - chr4_1:28248151-28248170 None:intergenic 40.0%
! TTTCAATACCCCTGGTGTTA+AGG + chr4_1:28248633-28248652 Msa0542860:three_prime_UTR 40.0%
!!! GGAGAACTGTCATTTTCTGA+AGG + chr4_1:28248654-28248673 Msa0542860:three_prime_UTR 40.0%
CTTCTTCAACAAAGCCTCCA+GGG + chr4_1:28246688-28246707 Msa0542860:CDS 45.0%
AGAGAAAGGGTGAATGGTGA+GGG - chr4_1:28246714-28246733 None:intergenic 45.0%
AAGAGAAAGGGTGAATGGTG+AGG - chr4_1:28246715-28246734 None:intergenic 45.0%
GTTGGAAGAGAAAGGGTGAA+TGG - chr4_1:28246720-28246739 None:intergenic 45.0%
!!! CCACGACTTTCTTTTTTCGG+GGG - chr4_1:28247819-28247838 None:intergenic 45.0%
GCTGCTACCAGTTTCCTTAA+GGG + chr4_1:28247856-28247875 Msa0542860:intron 45.0%
CCAACTGAGTTATGCTCACT+AGG - chr4_1:28248092-28248111 None:intergenic 45.0%
CCTAGTGAGCATAACTCAGT+TGG + chr4_1:28248089-28248108 Msa0542860:intron 45.0%
GTGAGCATAACTCAGTTGGT+AGG + chr4_1:28248093-28248112 Msa0542860:intron 45.0%
ATTGCAATATACAGGGGCCA+GGG + chr4_1:28248119-28248138 Msa0542860:intron 45.0%
GGGGTGAAATTCTATCCACT+AGG + chr4_1:28248176-28248195 Msa0542860:intron 45.0%
GATCGATGCTGCAGAGAAAA+AGG + chr4_1:28248513-28248532 Msa0542860:three_prime_UTR 45.0%
! GGGAAGTGGTGAATTTTCTC+TGG + chr4_1:28248567-28248586 Msa0542860:three_prime_UTR 45.0%
GACTTCTTGGTTGTAGCAGT+AGG + chr4_1:28248595-28248614 Msa0542860:three_prime_UTR 45.0%
ACAGTTCTCCTTAACACCAG+GGG - chr4_1:28248644-28248663 None:intergenic 45.0%
GACAGTTCTCCTTAACACCA+GGG - chr4_1:28248645-28248664 None:intergenic 45.0%
TGACAGTTCTCCTTAACACC+AGG - chr4_1:28248646-28248665 None:intergenic 45.0%
CTTCTTCAACAAAGCCTCCA+GGG + chr4_1:28246688-28246707 Msa0542860:CDS 45.0%
AGAGAAAGGGTGAATGGTGA+GGG - chr4_1:28246714-28246733 None:intergenic 45.0%
AAGAGAAAGGGTGAATGGTG+AGG - chr4_1:28246715-28246734 None:intergenic 45.0%
GTTGGAAGAGAAAGGGTGAA+TGG - chr4_1:28246720-28246739 None:intergenic 45.0%
!!! CCACGACTTTCTTTTTTCGG+GGG - chr4_1:28247819-28247838 None:intergenic 45.0%
GCTGCTACCAGTTTCCTTAA+GGG + chr4_1:28247856-28247875 Msa0542860:intron 45.0%
CCAACTGAGTTATGCTCACT+AGG - chr4_1:28248092-28248111 None:intergenic 45.0%
CCTAGTGAGCATAACTCAGT+TGG + chr4_1:28248089-28248108 Msa0542860:intron 45.0%
GTGAGCATAACTCAGTTGGT+AGG + chr4_1:28248093-28248112 Msa0542860:intron 45.0%
ATTGCAATATACAGGGGCCA+GGG + chr4_1:28248119-28248138 Msa0542860:intron 45.0%
GGGGTGAAATTCTATCCACT+AGG + chr4_1:28248176-28248195 Msa0542860:intron 45.0%
GATCGATGCTGCAGAGAAAA+AGG + chr4_1:28248513-28248532 Msa0542860:three_prime_UTR 45.0%
! GGGAAGTGGTGAATTTTCTC+TGG + chr4_1:28248567-28248586 Msa0542860:three_prime_UTR 45.0%
GACTTCTTGGTTGTAGCAGT+AGG + chr4_1:28248595-28248614 Msa0542860:three_prime_UTR 45.0%
ACAGTTCTCCTTAACACCAG+GGG - chr4_1:28248644-28248663 None:intergenic 45.0%
GACAGTTCTCCTTAACACCA+GGG - chr4_1:28248645-28248664 None:intergenic 45.0%
TGACAGTTCTCCTTAACACC+AGG - chr4_1:28248646-28248665 None:intergenic 45.0%
!! CCTGGAGGCTTTGTTGAAGA+AGG - chr4_1:28246690-28246709 None:intergenic 50.0%
CCTTCTTCAACAAAGCCTCC+AGG + chr4_1:28246687-28246706 Msa0542860:CDS 50.0%
ACTGGTCCCACCACTTTATG+TGG - chr4_1:28247278-28247297 None:intergenic 50.0%
CTTGCTCTTGGGAATTGCAG+TGG + chr4_1:28247727-28247746 Msa0542860:intron 50.0%
CCCCCGAAAAAAGAAAGTCG+TGG + chr4_1:28247816-28247835 Msa0542860:intron 50.0%
AAAGTCGTGGTCCTTGGAAC+TGG + chr4_1:28247829-28247848 Msa0542860:intron 50.0%
GGCTGCTACCAGTTTCCTTA+AGG + chr4_1:28247855-28247874 Msa0542860:intron 50.0%
ACTGACCCACAAGTGACACT+CGG - chr4_1:28247952-28247971 None:intergenic 50.0%
CTTGTGGGTCAGTTGAAGCA+CGG + chr4_1:28247959-28247978 Msa0542860:intron 50.0%
CATTGCAATATACAGGGGCC+AGG + chr4_1:28248118-28248137 Msa0542860:intron 50.0%
!! CCTGGAGGCTTTGTTGAAGA+AGG - chr4_1:28246690-28246709 None:intergenic 50.0%
CCTTCTTCAACAAAGCCTCC+AGG + chr4_1:28246687-28246706 Msa0542860:CDS 50.0%
ACTGGTCCCACCACTTTATG+TGG - chr4_1:28247278-28247297 None:intergenic 50.0%
CTTGCTCTTGGGAATTGCAG+TGG + chr4_1:28247727-28247746 Msa0542860:intron 50.0%
CCCCCGAAAAAAGAAAGTCG+TGG + chr4_1:28247816-28247835 Msa0542860:intron 50.0%
AAAGTCGTGGTCCTTGGAAC+TGG + chr4_1:28247829-28247848 Msa0542860:intron 50.0%
GGCTGCTACCAGTTTCCTTA+AGG + chr4_1:28247855-28247874 Msa0542860:intron 50.0%
ACTGACCCACAAGTGACACT+CGG - chr4_1:28247952-28247971 None:intergenic 50.0%
CTTGTGGGTCAGTTGAAGCA+CGG + chr4_1:28247959-28247978 Msa0542860:intron 50.0%
CATTGCAATATACAGGGGCC+AGG + chr4_1:28248118-28248137 Msa0542860:intron 50.0%
GCTCTTGGGAATTGCAGTGG+TGG + chr4_1:28247730-28247749 Msa0542860:intron 55.0%
CTTGGGAATTGCAGTGGTGG+AGG + chr4_1:28247733-28247752 Msa0542860:intron 55.0%
! GAATTGCAGTGGTGGAGGTG+TGG + chr4_1:28247738-28247757 Msa0542860:intron 55.0%
TCGTGGTCCTTGGAACTGGA+TGG + chr4_1:28247833-28247852 Msa0542860:intron 55.0%
CGTGGTCCTTGGAACTGGAT+GGG + chr4_1:28247834-28247853 Msa0542860:intron 55.0%
TAGCAGCCCATCCAGTTCCA+AGG - chr4_1:28247843-28247862 None:intergenic 55.0%
!! AGCACGGAGCATCGTAGAAC+CGG + chr4_1:28247975-28247994 Msa0542860:intron 55.0%
GCTCTTGGGAATTGCAGTGG+TGG + chr4_1:28247730-28247749 Msa0542860:intron 55.0%
CTTGGGAATTGCAGTGGTGG+AGG + chr4_1:28247733-28247752 Msa0542860:intron 55.0%
! GAATTGCAGTGGTGGAGGTG+TGG + chr4_1:28247738-28247757 Msa0542860:intron 55.0%
TCGTGGTCCTTGGAACTGGA+TGG + chr4_1:28247833-28247852 Msa0542860:intron 55.0%
CGTGGTCCTTGGAACTGGAT+GGG + chr4_1:28247834-28247853 Msa0542860:intron 55.0%
TAGCAGCCCATCCAGTTCCA+AGG - chr4_1:28247843-28247862 None:intergenic 55.0%
!! AGCACGGAGCATCGTAGAAC+CGG + chr4_1:28247975-28247994 Msa0542860:intron 55.0%
! TTGCAGTGGTGGAGGTGTGG+TGG + chr4_1:28247741-28247760 Msa0542860:intron 60.0%
GGAATCCCAGGTTCGAACCC+TGG - chr4_1:28248139-28248158 None:intergenic 60.0%
! TTGCAGTGGTGGAGGTGTGG+TGG + chr4_1:28247741-28247760 Msa0542860:intron 60.0%
GGAATCCCAGGTTCGAACCC+TGG - chr4_1:28248139-28248158 None:intergenic 60.0%
GTGAATGGTGAGGGCCCTGG+AGG - chr4_1:28246705-28246724 None:intergenic 65.0%
AGGGTGAATGGTGAGGGCCC+TGG - chr4_1:28246708-28246727 None:intergenic 65.0%
AGGGGCCAGGGTTCGAACCT+GGG + chr4_1:28248131-28248150 Msa0542860:intron 65.0%
GTGAATGGTGAGGGCCCTGG+AGG - chr4_1:28246705-28246724 None:intergenic 65.0%
AGGGTGAATGGTGAGGGCCC+TGG - chr4_1:28246708-28246727 None:intergenic 65.0%
AGGGGCCAGGGTTCGAACCT+GGG + chr4_1:28248131-28248150 Msa0542860:intron 65.0%
CAGGGGCCAGGGTTCGAACC+TGG + chr4_1:28248130-28248149 Msa0542860:intron 70.0%
CAGGGGCCAGGGTTCGAACC+TGG + chr4_1:28248130-28248149 Msa0542860:intron 70.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 28246548 28248794 28246548 ID=Msa0542860;Name=Msa0542860
chr4_1 mRNA 28246548 28248794 28246548 ID=Msa0542860-mRNA-1;Parent=Msa0542860;Name=Msa0542860-mRNA-1;_AED=0.17;_eAED=0.17;_QI=126|0|1|1|0|1|2|307|34
chr4_1 exon 28246548 28246762 28246548 ID=Msa0542860-mRNA-1:exon:7648;Parent=Msa0542860-mRNA-1
chr4_1 exon 28248472 28248794 28248472 ID=Msa0542860-mRNA-1:exon:7649;Parent=Msa0542860-mRNA-1
chr4_1 five_prime_UTR 28246548 28246673 28246548 ID=Msa0542860-mRNA-1:five_prime_utr;Parent=Msa0542860-mRNA-1
chr4_1 CDS 28246674 28246762 28246674 ID=Msa0542860-mRNA-1:cds;Parent=Msa0542860-mRNA-1
chr4_1 CDS 28248472 28248487 28248472 ID=Msa0542860-mRNA-1:cds;Parent=Msa0542860-mRNA-1
chr4_1 three_prime_UTR 28248488 28248794 28248488 ID=Msa0542860-mRNA-1:three_prime_utr;Parent=Msa0542860-mRNA-1
Gene Sequence

>Msa0542860

ATGACGATTGGTTCCTTCTTCAACAAAGCCTCCAGGGCCCTCACCATTCACCCTTTCTCTTCCAACTTTCTGCTTCTTTGTACTATCAGCGAAATGGAGAAATAA
Protein sequence

>Msa0542860

MTIGSFFNKASRALTIHPFSSNFLLLCTISEMEK*