AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0543650

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0543650 MtrunA17_Chr4g0023991 96.473 482 11 1 1 482 1 476 0.0 967
Msa0543650 MtrunA17_Chr2g0322991 63.505 485 149 7 9 482 6 473 0.0 582
Msa0543650 MtrunA17_Chr4g0024001 51.471 476 205 8 4 479 26 475 9.19e-162 466
Msa0543650 MtrunA17_Chr8g0348511 43.789 475 245 10 16 479 24 487 1.49e-127 379
Msa0543650 MtrunA17_Chr8g0387371 44.706 425 211 8 63 478 105 514 1.19e-124 373
Msa0543650 MtrunA17_Chr2g0327551 44.651 430 211 8 59 478 41 453 2.74e-123 367
Msa0543650 MtrunA17_Chr7g0239421 42.989 435 211 9 63 479 92 507 1.01e-120 362
Msa0543650 MtrunA17_Chr3g0112211 41.014 434 241 5 48 479 67 487 2.02e-119 358
Msa0543650 MtrunA17_Chr4g0030331 45.113 399 211 6 52 446 97 491 8.94e-118 355
Msa0543650 MtrunA17_Chr5g0395861 37.237 427 248 6 59 477 121 535 1.67e-94 296
Msa0543650 MtrunA17_Chr5g0397301 50.455 220 106 3 45 263 63 280 4.38e-69 223
Msa0543650 MtrunA17_Chr7g0248241 54.229 201 90 2 63 262 91 290 3.20e-68 221
Msa0543650 MtrunA17_Chr5g0397311 40.000 220 117 7 264 478 22 231 6.16e-42 149
Msa0543650 MtrunA17_Chr7g0248231 39.053 169 99 3 264 429 4 171 6.26e-35 129
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0543650 AT1G05280.1 54.375 480 196 9 13 482 24 490 0.0 523
Msa0543650 AT1G05280.2 54.920 437 182 7 13 441 24 453 3.57e-168 483
Msa0543650 AT4G15240.1 45.788 463 239 6 18 479 33 484 3.57e-144 422
Msa0543650 AT1G01570.1 44.714 454 232 8 37 478 26 472 3.36e-128 381
Msa0543650 AT1G01570.2 44.714 454 232 8 37 478 57 503 1.30e-127 380
Msa0543650 AT4G23490.1 46.977 430 198 10 63 478 109 522 2.36e-126 378
Msa0543650 AT5G41460.1 45.519 424 214 8 62 479 109 521 1.48e-123 371
Msa0543650 AT4G11350.1 42.480 492 236 13 13 478 33 503 3.28e-122 367
Msa0543650 AT2G37730.1 39.829 467 264 5 12 471 45 501 2.33e-116 352
Msa0543650 AT1G07850.1 44.917 423 215 7 62 478 122 532 5.01e-113 344
Msa0543650 AT4G00295.1 49.267 341 163 5 60 392 75 413 4.88e-108 329
Msa0543650 AT3G11420.1 39.394 429 234 9 63 479 91 505 8.70e-108 330
Msa0543650 AT1G33250.1 39.286 420 244 6 58 473 124 536 6.60e-105 323
Msa0543650 AT5G12460.1 34.722 432 248 8 59 479 32 440 1.54e-86 273
Msa0543650 AT4G11350.2 50.673 223 102 3 56 271 80 301 1.97e-68 222

Find 99 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGATAGTGTAAAAGTATT+AGG 0.139151 4_1:+30221137 Msa0543650:three_prime_UTR
GAAAATGAATATTGTGAATA+TGG 0.201776 4_1:-30218403 None:intergenic
AGTTGATGAGATTGTAAATT+AGG 0.208385 4_1:+30221064 Msa0543650:three_prime_UTR
CAGAATCGGTTGTTTGGTTT+TGG 0.208966 4_1:+30219044 Msa0543650:CDS
ACACTATCAGAGAAACAAAA+AGG 0.228221 4_1:-30221125 None:intergenic
AACATTCAAGCAGTGGAAAA+AGG 0.233460 4_1:+30220087 Msa0543650:CDS
TTCATCTCTTGCTAAGGTTT+TGG 0.236987 4_1:+30219100 Msa0543650:CDS
ACCCTTGATACATTATATAT+TGG 0.239113 4_1:-30221032 None:intergenic
TCAAAATTGCTCAAAGGATT+TGG 0.247836 4_1:+30220249 Msa0543650:CDS
TTGATGACTTCTAGTTTCTT+TGG 0.255302 4_1:-30220277 None:intergenic
ATGAAACAGGTTGATTTAAC+TGG 0.275394 4_1:+30219794 Msa0543650:intron
GAAAGGTATCCTCATCTATA+TGG 0.276631 4_1:+30219146 Msa0543650:CDS
ATAATTCCATGTGAAAATTA+AGG 0.278129 4_1:-30218266 None:intergenic
ACATCACAACAATGCCTTCT+TGG 0.300949 4_1:-30220895 None:intergenic
GTATTATAAACAGTTATTAT+AGG 0.313324 4_1:+30220218 Msa0543650:CDS
CATGAGCTTTGGTATTATAT+TGG 0.323131 4_1:+30218999 Msa0543650:CDS
GCCAACCGTGAAAATCTAAT+TGG 0.336073 4_1:+30218180 Msa0543650:five_prime_UTR
TTACTGGATGCGCAGCTAAT+AGG 0.342798 4_1:-30219829 None:intergenic
GTTTCTCACGCCGTGGATTA+CGG 0.343663 4_1:+30218325 Msa0543650:five_prime_UTR
ACTCTAGATGATATTGTGTT+TGG 0.352904 4_1:+30218645 Msa0543650:CDS
CTTGTATCAGTACTAATATT+AGG 0.360904 4_1:+30218540 Msa0543650:CDS
GTAGTCATGTTTGGAAAGAT+TGG 0.362157 4_1:-30219890 None:intergenic
GATTTAACTGGAAATACCTT+TGG 0.367215 4_1:+30219806 Msa0543650:CDS
CTGATAGTGTAAAAGTATTA+GGG 0.371530 4_1:+30221138 Msa0543650:three_prime_UTR
ACAACCCTTCATTGCATTGT+TGG 0.373372 4_1:-30218730 None:intergenic
AATGAAGGGTTGTGTGTTCT+TGG 0.376173 4_1:+30218740 Msa0543650:CDS
AGAGACAGTAAGGGAGTTAC+TGG 0.382398 4_1:-30219845 None:intergenic
CAAAATATGATCATGAGCTT+TGG 0.385050 4_1:+30218988 Msa0543650:CDS
TTGCTTCAAGCAAGAGTTCA+TGG 0.388430 4_1:+30218670 Msa0543650:CDS
TATTTCGATAGCGTCTCTTC+TGG 0.390501 4_1:+30220187 Msa0543650:CDS
TATGAGCAGAATCGGTTGTT+TGG 0.395496 4_1:+30219038 Msa0543650:CDS
TAGTTACCTGTTTGATATCA+AGG 0.395977 4_1:-30220321 None:intergenic
TACTCTTGCTTAGCTGAACT+TGG 0.401160 4_1:+30219185 Msa0543650:CDS
AAAACACTTGGTCACATCAT+AGG 0.404875 4_1:-30221004 None:intergenic
GAAAGGAGTATGTGAAACTA+TGG 0.414791 4_1:+30218700 Msa0543650:CDS
CTTCACATTTGAATGATTCA+AGG 0.414862 4_1:-30218904 None:intergenic
CTCAAGTGCTAGTGACCAAC+TGG 0.419998 4_1:+30220924 Msa0543650:CDS
ATCCAATATATAATGTATCA+AGG 0.420906 4_1:+30221030 Msa0543650:three_prime_UTR
CATGGACAATTTCAGTGTCA+TGG 0.422607 4_1:+30220002 Msa0543650:CDS
GCTTGTGTAGTAGTCATGTT+TGG 0.424081 4_1:-30219899 None:intergenic
AAGATTGGATCTGTGTAATC+TGG 0.442009 4_1:-30219875 None:intergenic
CTGAGATTTATGAGCAGAAT+CGG 0.442403 4_1:+30219030 Msa0543650:CDS
GAGGACTTAGATCAGCTATC+CGG 0.449769 4_1:+30218853 Msa0543650:CDS
GAAAACCAACAATGCAATGA+AGG 0.452615 4_1:+30218725 Msa0543650:CDS
ATGGACAATTTCAGTGTCAT+GGG 0.456001 4_1:+30220003 Msa0543650:CDS
AATGTGAAGTGGTATGTGTT+TGG 0.460214 4_1:+30218918 Msa0543650:CDS
CATAGAGGAGGAAGAGAAGA+AGG 0.462515 4_1:-30218789 None:intergenic
AGAGTCTTCACCAAATTCTC+CGG 0.470157 4_1:-30218963 None:intergenic
TGGATGATGCAGAGACAGTA+AGG 0.476779 4_1:-30219855 None:intergenic
TCGAAACCGTGAAGTGTCTT+CGG 0.481952 4_1:-30218817 None:intergenic
GCTAGACCTTGATATCAAAC+AGG 0.484491 4_1:+30220315 Msa0543650:CDS
TTAGTTACATCTTCAGTTTG+TGG 0.488441 4_1:+30218507 Msa0543650:CDS
TATTAGTTCATCTCTTGCTA+AGG 0.494347 4_1:+30219094 Msa0543650:CDS
CAGTTGGTCACTAGCACTTG+AGG 0.504072 4_1:-30220923 None:intergenic
GTGTCTCCGAAGACACTTCA+CGG 0.511436 4_1:+30218811 Msa0543650:CDS
CGGTTTCGATATACTTGCAA+AGG 0.513737 4_1:+30218831 Msa0543650:CDS
AGCGTCTCTTCTGGTAAAGA+TGG 0.520299 4_1:+30220196 Msa0543650:CDS
CCGCGACAACACGCGCCACC+CGG 0.523774 4_1:-30218872 None:intergenic
CTCTAACAGCAATTTCCAGT+TGG 0.524734 4_1:-30220939 None:intergenic
CCGGGTGGCGCGTGTTGTCG+CGG 0.525441 4_1:+30218872 Msa0543650:CDS
AGGACTTAGATCAGCTATCC+GGG 0.525783 4_1:+30218854 Msa0543650:CDS
TTTGTTACCTGATGAAAACC+TGG 0.528524 4_1:-30219227 None:intergenic
AAACAATTTGCTATGACAGA+TGG 0.528616 4_1:+30219975 Msa0543650:CDS
TCTCGCGCATTCACAACATC+CGG 0.534501 4_1:-30220064 None:intergenic
AAAGGTATCCTCATCTATAT+GGG 0.534969 4_1:+30219147 Msa0543650:CDS
TGCTTAGCTGAACTTGGTGT+AGG 0.536354 4_1:+30219191 Msa0543650:CDS
GAGTTCATGGACAAAGAGAA+AGG 0.541475 4_1:+30218683 Msa0543650:CDS
TCCATGTGAAAATTAAGGTA+AGG 0.547198 4_1:-30218261 None:intergenic
TTACTCAAAACGTCAAAGCT+AGG 0.548214 4_1:+30218292 Msa0543650:five_prime_UTR
AGTTGGTCACTAGCACTTGA+GGG 0.550589 4_1:-30220922 None:intergenic
GAGTCTTCACCAAATTCTCC+GGG 0.555356 4_1:-30218962 None:intergenic
TGTGAAAATTAAGGTAAGGT+AGG 0.557527 4_1:-30218257 None:intergenic
CGCGAGAAACATTCAAGCAG+TGG 0.559585 4_1:+30220080 Msa0543650:CDS
TTCAGTTGCAGGCTCCAAGA+AGG 0.560994 4_1:+30220881 Msa0543650:intron
CCTCATCTATATGGGAGTGA+TGG 0.561800 4_1:+30219155 Msa0543650:CDS
AGGAGTATGTGAAACTATGG+TGG 0.566032 4_1:+30218703 Msa0543650:CDS
TCCAATATATAATGTATCAA+GGG 0.567438 4_1:+30221031 Msa0543650:three_prime_UTR
TTACAAGGATCAGGGTGAAG+TGG 0.568617 4_1:-30220148 None:intergenic
ATCTATATGGGAGTGATGGT+AGG 0.569346 4_1:+30219159 Msa0543650:CDS
TCTATATGGGAGTGATGGTA+GGG 0.573257 4_1:+30219160 Msa0543650:CDS
TGAATCATTCAAATGTGAAG+TGG 0.573727 4_1:+30218907 Msa0543650:CDS
GTTTGTTGTTTCTCACGCCG+TGG 0.598986 4_1:+30218318 Msa0543650:five_prime_UTR
TTTCGATATACTTGCAAAGG+AGG 0.604442 4_1:+30218834 Msa0543650:CDS
GAAACTAGAAGTCATCAAAG+TGG 0.613370 4_1:+30220282 Msa0543650:CDS
GTGTCTTCGGAGACACATAG+AGG 0.621246 4_1:-30218804 None:intergenic
GTAGGATTGACACATGAACC+AGG 0.628075 4_1:+30219209 Msa0543650:CDS
TGGACAATTTCAGTGTCATG+GGG 0.639158 4_1:+30220004 Msa0543650:CDS
CCATCACTCCCATATAGATG+AGG 0.641487 4_1:-30219155 None:intergenic
AAAACCAACAATGCAATGAA+GGG 0.642497 4_1:+30218726 Msa0543650:CDS
AAGTGAACCACCGTAATCCA+CGG 0.643832 4_1:-30218335 None:intergenic
ATAGAGGAGGAAGAGAAGAA+GGG 0.654986 4_1:-30218788 None:intergenic
GGATGATGCAGAGACAGTAA+GGG 0.659335 4_1:-30219854 None:intergenic
CAGTAAATGTTGATTCTCAG+AGG 0.666403 4_1:+30219942 Msa0543650:CDS
TGTGTTTGGAGACGATGACA+CGG 0.671539 4_1:+30218932 Msa0543650:CDS
GCGTGTTGTCGCGGAGACTG+TGG 0.676360 4_1:+30218881 Msa0543650:CDS
ATGCGCAGCTAATAGGCCAA+AGG 0.676747 4_1:-30219822 None:intergenic
TCTCACGCCGTGGATTACGG+TGG 0.681097 4_1:+30218328 Msa0543650:five_prime_UTR
TCTTCGGAGACACATAGAGG+AGG 0.694314 4_1:-30218801 None:intergenic
ACTTAGATCAGCTATCCGGG+TGG 0.754943 4_1:+30218857 Msa0543650:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTGAGTAATTTATTTT+TGG + chr4_1:30219287-30219306 Msa0543650:intron 10.0%
!!! ATTTTCAATCTTTTTTTTGA+AGG + chr4_1:30218210-30218229 Msa0543650:five_prime_UTR 15.0%
!! GTATTATAAACAGTTATTAT+AGG + chr4_1:30220218-30220237 Msa0543650:CDS 15.0%
!!! TTTTTGAAATGCATTTTTTT+TGG + chr4_1:30220418-30220437 Msa0543650:intron 15.0%
!! ATAATTCCATGTGAAAATTA+AGG - chr4_1:30218269-30218288 None:intergenic 20.0%
!! GAAAATGAATATTGTGAATA+TGG - chr4_1:30218406-30218425 None:intergenic 20.0%
!!! AGTTGTGTTTGTTTTTTTTA+TGG - chr4_1:30218628-30218647 None:intergenic 20.0%
!!! TTTGAGTAATTTATTTTTGG+TGG + chr4_1:30219290-30219309 Msa0543650:intron 20.0%
!!! ACAGATCATTTTAGATTTTA+TGG + chr4_1:30219685-30219704 Msa0543650:intron 20.0%
!! ACAAAAACTATGCATTAATT+AGG - chr4_1:30219765-30219784 None:intergenic 20.0%
!!! TAATTAATGCATAGTTTTTG+TGG + chr4_1:30219764-30219783 Msa0543650:intron 20.0%
!! CAAGATATACCATAATTAAA+CGG - chr4_1:30220356-30220375 None:intergenic 20.0%
!! TTTGCTGATAAAATAAGATA+AGG + chr4_1:30220384-30220403 Msa0543650:intron 20.0%
!! AAATGCATTTCAAAAACTTT+CGG - chr4_1:30220414-30220433 None:intergenic 20.0%
!!! AACATTGTTTTTCTTGTTTA+TGG + chr4_1:30220659-30220678 Msa0543650:intron 20.0%
!! ATATTGGATTAAAAAACACT+TGG - chr4_1:30221019-30221038 None:intergenic 20.0%
!! ATCCAATATATAATGTATCA+AGG + chr4_1:30221030-30221049 Msa0543650:three_prime_UTR 20.0%
!! TCCAATATATAATGTATCAA+GGG + chr4_1:30221031-30221050 Msa0543650:three_prime_UTR 20.0%
! CTTGTATCAGTACTAATATT+AGG + chr4_1:30218540-30218559 Msa0543650:CDS 25.0%
!!! TAAGGTTTTTGATTCTTGTT+TGG + chr4_1:30219124-30219143 Msa0543650:CDS 25.0%
!!! TTTTTGATTCTTGTTTGGAA+AGG + chr4_1:30219129-30219148 Msa0543650:CDS 25.0%
!!! GTAATTTATTTTTGGTGGAA+TGG + chr4_1:30219295-30219314 Msa0543650:intron 25.0%
! TTTCTCTTTCGTTAATTTCA+GGG - chr4_1:30219366-30219385 None:intergenic 25.0%
! ATTTCTCTTTCGTTAATTTC+AGG - chr4_1:30219367-30219386 None:intergenic 25.0%
!! TTGATTGGTTAAAATTCATG+TGG + chr4_1:30219419-30219438 Msa0543650:intron 25.0%
!! TGATTGGTTAAAATTCATGT+GGG + chr4_1:30219420-30219439 Msa0543650:intron 25.0%
!! TTGATTGGTTAAAATTCACA+TGG - chr4_1:30219487-30219506 None:intergenic 25.0%
!! ATTTTATGGACCAAATAACT+CGG + chr4_1:30219699-30219718 Msa0543650:intron 25.0%
!! AAGATAGTAGATCTTTTACA+AGG - chr4_1:30220166-30220185 None:intergenic 25.0%
!! ATCTTTTCAAAATTGCTCAA+AGG + chr4_1:30220243-30220262 Msa0543650:CDS 25.0%
! TTTGTGAATCCGTTTAATTA+TGG + chr4_1:30220344-30220363 Msa0543650:intron 25.0%
!!! TTTTTTGGTAAACCTGTTAT+TGG + chr4_1:30220433-30220452 Msa0543650:intron 25.0%
! AAAAACCCTGAAAAAATTTG+TGG - chr4_1:30220811-30220830 None:intergenic 25.0%
!!! CAAATTTTTTCAGGGTTTTT+AGG + chr4_1:30220811-30220830 Msa0543650:intron 25.0%
!!! TTTTATTTGATTTCAGTTGC+AGG + chr4_1:30220870-30220889 Msa0543650:intron 25.0%
! ACCCTTGATACATTATATAT+TGG - chr4_1:30221035-30221054 None:intergenic 25.0%
!!! AGTTGATGAGATTGTAAATT+AGG + chr4_1:30221064-30221083 Msa0543650:three_prime_UTR 25.0%
!!! TCTGATAGTGTAAAAGTATT+AGG + chr4_1:30221137-30221156 Msa0543650:three_prime_UTR 25.0%
!!! CTGATAGTGTAAAAGTATTA+GGG + chr4_1:30221138-30221157 Msa0543650:three_prime_UTR 25.0%
! CTCTACCAATTAGATTTTCA+CGG - chr4_1:30218188-30218207 None:intergenic 30.0%
TGTGAAAATTAAGGTAAGGT+AGG - chr4_1:30218260-30218279 None:intergenic 30.0%
! ACCTTACCTTAATTTTCACA+TGG + chr4_1:30218260-30218279 Msa0543650:five_prime_UTR 30.0%
TCCATGTGAAAATTAAGGTA+AGG - chr4_1:30218264-30218283 None:intergenic 30.0%
TTAGTTACATCTTCAGTTTG+TGG + chr4_1:30218507-30218526 Msa0543650:CDS 30.0%
ACTCTAGATGATATTGTGTT+TGG + chr4_1:30218645-30218664 Msa0543650:CDS 30.0%
AAAACCAACAATGCAATGAA+GGG + chr4_1:30218726-30218745 Msa0543650:CDS 30.0%
CTTCACATTTGAATGATTCA+AGG - chr4_1:30218907-30218926 None:intergenic 30.0%
TGAATCATTCAAATGTGAAG+TGG + chr4_1:30218907-30218926 Msa0543650:CDS 30.0%
CAAAATATGATCATGAGCTT+TGG + chr4_1:30218988-30219007 Msa0543650:CDS 30.0%
! CATGAGCTTTGGTATTATAT+TGG + chr4_1:30218999-30219018 Msa0543650:CDS 30.0%
TATTAGTTCATCTCTTGCTA+AGG + chr4_1:30219094-30219113 Msa0543650:CDS 30.0%
!! AAAGGTATCCTCATCTATAT+GGG + chr4_1:30219147-30219166 Msa0543650:CDS 30.0%
! TCTAACACACTCTTTTTGAT+TGG - chr4_1:30219502-30219521 None:intergenic 30.0%
ATATGAGAATTTCGTTAACG+AGG - chr4_1:30219557-30219576 None:intergenic 30.0%
AACAAAAATAACCATCCAGA+AGG - chr4_1:30219614-30219633 None:intergenic 30.0%
!! ATTCTAGAATCCGAGTTATT+TGG - chr4_1:30219712-30219731 None:intergenic 30.0%
CATTAATTAGGCTCGTTAAA+GGG - chr4_1:30219753-30219772 None:intergenic 30.0%
TTGTGGTTATGAAATGAAAC+AGG + chr4_1:30219781-30219800 Msa0543650:intron 30.0%
!! ATGAAACAGGTTGATTTAAC+TGG + chr4_1:30219794-30219813 Msa0543650:intron 30.0%
GATTTAACTGGAAATACCTT+TGG + chr4_1:30219806-30219825 Msa0543650:CDS 30.0%
!! AAACAATTTGCTATGACAGA+TGG + chr4_1:30219975-30219994 Msa0543650:CDS 30.0%
! TAGATCTTTTACAAGGATCA+GGG - chr4_1:30220159-30220178 None:intergenic 30.0%
TCAAAATTGCTCAAAGGATT+TGG + chr4_1:30220249-30220268 Msa0543650:CDS 30.0%
!! TTGATGACTTCTAGTTTCTT+TGG - chr4_1:30220280-30220299 None:intergenic 30.0%
TAGTTACCTGTTTGATATCA+AGG - chr4_1:30220324-30220343 None:intergenic 30.0%
!!! TCAGTTTTAACTTCGTGTTT+GGG - chr4_1:30220763-30220782 None:intergenic 30.0%
!!! TTCAGTTTTAACTTCGTGTT+TGG - chr4_1:30220764-30220783 None:intergenic 30.0%
!!! ACGAATCCACAAATTTTTTC+AGG + chr4_1:30220802-30220821 Msa0543650:intron 30.0%
!!! CGAATCCACAAATTTTTTCA+GGG + chr4_1:30220803-30220822 Msa0543650:intron 30.0%
!!! ATTTTTTCAGGGTTTTTAGG+AGG + chr4_1:30220814-30220833 Msa0543650:intron 30.0%
ACTATCAGAGAAACAAAAAG+GGG - chr4_1:30221126-30221145 None:intergenic 30.0%
CACTATCAGAGAAACAAAAA+GGG - chr4_1:30221127-30221146 None:intergenic 30.0%
ACACTATCAGAGAAACAAAA+AGG - chr4_1:30221128-30221147 None:intergenic 30.0%
! ACCAATTAGATTTTCACGGT+TGG - chr4_1:30218184-30218203 None:intergenic 35.0%
TTACTCAAAACGTCAAAGCT+AGG + chr4_1:30218292-30218311 Msa0543650:five_prime_UTR 35.0%
! GAAAGGAGTATGTGAAACTA+TGG + chr4_1:30218700-30218719 Msa0543650:CDS 35.0%
GAAAACCAACAATGCAATGA+AGG + chr4_1:30218725-30218744 Msa0543650:CDS 35.0%
! GAAGGGTCATATTCATTTTG+TGG - chr4_1:30218774-30218793 None:intergenic 35.0%
TTTCGATATACTTGCAAAGG+AGG + chr4_1:30218834-30218853 Msa0543650:CDS 35.0%
AATGTGAAGTGGTATGTGTT+TGG + chr4_1:30218918-30218937 Msa0543650:CDS 35.0%
CTGAGATTTATGAGCAGAAT+CGG + chr4_1:30219030-30219049 Msa0543650:CDS 35.0%
!!! TTTGGTTTTGGAATGGCTTT+TGG + chr4_1:30219056-30219075 Msa0543650:CDS 35.0%
! TTCATCTCTTGCTAAGGTTT+TGG + chr4_1:30219100-30219119 Msa0543650:CDS 35.0%
!! GAAAGGTATCCTCATCTATA+TGG + chr4_1:30219146-30219165 Msa0543650:CDS 35.0%
TTTGTTACCTGATGAAAACC+TGG - chr4_1:30219230-30219249 None:intergenic 35.0%
TTCAACACACTCTCTTTGAT+TGG + chr4_1:30219404-30219423 Msa0543650:intron 35.0%
ATGTGGGTCCCATATAAATT+TGG - chr4_1:30219451-30219470 None:intergenic 35.0%
TCTGTTTACATTAGTCCTTC+TGG + chr4_1:30219596-30219615 Msa0543650:intron 35.0%
TTTACATTAGTCCTTCTGGA+TGG + chr4_1:30219600-30219619 Msa0543650:intron 35.0%
GCATTAATTAGGCTCGTTAA+AGG - chr4_1:30219754-30219773 None:intergenic 35.0%
AAGATTGGATCTGTGTAATC+TGG - chr4_1:30219878-30219897 None:intergenic 35.0%
GTAGTCATGTTTGGAAAGAT+TGG - chr4_1:30219893-30219912 None:intergenic 35.0%
!! CAGTAAATGTTGATTCTCAG+AGG + chr4_1:30219942-30219961 Msa0543650:CDS 35.0%
ATGGACAATTTCAGTGTCAT+GGG + chr4_1:30220003-30220022 Msa0543650:CDS 35.0%
TCCGGTAAAAACATATGGTT+GGG - chr4_1:30220049-30220068 None:intergenic 35.0%
ATCCGGTAAAAACATATGGT+TGG - chr4_1:30220050-30220069 None:intergenic 35.0%
CAACATCCGGTAAAAACATA+TGG - chr4_1:30220054-30220073 None:intergenic 35.0%
AACATTCAAGCAGTGGAAAA+AGG + chr4_1:30220087-30220106 Msa0543650:CDS 35.0%
ACATTCAAGCAGTGGAAAAA+GGG + chr4_1:30220088-30220107 Msa0543650:CDS 35.0%
! GTAGATCTTTTACAAGGATC+AGG - chr4_1:30220160-30220179 None:intergenic 35.0%
GAAACTAGAAGTCATCAAAG+TGG + chr4_1:30220282-30220301 Msa0543650:CDS 35.0%
GAATACTTAGCACCAATAAC+AGG - chr4_1:30220448-30220467 None:intergenic 35.0%
CAAGATCTCAGGTTCAAATT+TGG - chr4_1:30220538-30220557 None:intergenic 35.0%
GTACATTACTGAGAGTGAAA+TGG - chr4_1:30220577-30220596 None:intergenic 35.0%
TCACTCTCAGTAATGTACAT+CGG + chr4_1:30220579-30220598 Msa0543650:intron 35.0%
AAAACACTTGGTCACATCAT+AGG - chr4_1:30221007-30221026 None:intergenic 35.0%
GCCAACCGTGAAAATCTAAT+TGG + chr4_1:30218180-30218199 Msa0543650:five_prime_UTR 40.0%
TTGCTTCAAGCAAGAGTTCA+TGG + chr4_1:30218670-30218689 Msa0543650:CDS 40.0%
GAGTTCATGGACAAAGAGAA+AGG + chr4_1:30218683-30218702 Msa0543650:CDS 40.0%
! AGGAGTATGTGAAACTATGG+TGG + chr4_1:30218703-30218722 Msa0543650:CDS 40.0%
ACAACCCTTCATTGCATTGT+TGG - chr4_1:30218733-30218752 None:intergenic 40.0%
AATGAAGGGTTGTGTGTTCT+TGG + chr4_1:30218740-30218759 Msa0543650:CDS 40.0%
ATAGAGGAGGAAGAGAAGAA+GGG - chr4_1:30218791-30218810 None:intergenic 40.0%
CGGTTTCGATATACTTGCAA+AGG + chr4_1:30218831-30218850 Msa0543650:CDS 40.0%
!!! GGTTTTTTTCCCGGAGAATT+TGG + chr4_1:30218953-30218972 Msa0543650:CDS 40.0%
AGAGTCTTCACCAAATTCTC+CGG - chr4_1:30218966-30218985 None:intergenic 40.0%
TATGAGCAGAATCGGTTGTT+TGG + chr4_1:30219038-30219057 Msa0543650:CDS 40.0%
!! CAGAATCGGTTGTTTGGTTT+TGG + chr4_1:30219044-30219063 Msa0543650:CDS 40.0%
!!! TCGGTTGTTTGGTTTTGGAA+TGG + chr4_1:30219049-30219068 Msa0543650:CDS 40.0%
!!! TCTTGCTAAGGTTTTGGCTA+AGG + chr4_1:30219106-30219125 Msa0543650:CDS 40.0%
ATCTATATGGGAGTGATGGT+AGG + chr4_1:30219159-30219178 Msa0543650:CDS 40.0%
TCTATATGGGAGTGATGGTA+GGG + chr4_1:30219160-30219179 Msa0543650:CDS 40.0%
TACTCTTGCTTAGCTGAACT+TGG + chr4_1:30219185-30219204 Msa0543650:CDS 40.0%
! ACATGAACCAGGTTTTCATC+AGG + chr4_1:30219220-30219239 Msa0543650:CDS 40.0%
TGGGACCCACCAAATTTATA+TGG + chr4_1:30219439-30219458 Msa0543650:intron 40.0%
GGGACCCACCAAATTTATAT+GGG + chr4_1:30219440-30219459 Msa0543650:intron 40.0%
GGGTCCCATATAAATTTGGT+GGG - chr4_1:30219447-30219466 None:intergenic 40.0%
TGGGTCCCATATAAATTTGG+TGG - chr4_1:30219448-30219467 None:intergenic 40.0%
TATATGGGACCCACATGATT+TGG + chr4_1:30219455-30219474 Msa0543650:intron 40.0%
TAATTAGGCTCGTTAAAGGG+AGG - chr4_1:30219750-30219769 None:intergenic 40.0%
GCTTGTGTAGTAGTCATGTT+TGG - chr4_1:30219902-30219921 None:intergenic 40.0%
! GCTATGACAGATGGTTTTCA+TGG + chr4_1:30219984-30220003 Msa0543650:CDS 40.0%
CATGGACAATTTCAGTGTCA+TGG + chr4_1:30220002-30220021 Msa0543650:CDS 40.0%
TGGACAATTTCAGTGTCATG+GGG + chr4_1:30220004-30220023 Msa0543650:CDS 40.0%
CCGGTAAAAACATATGGTTG+GGG - chr4_1:30220048-30220067 None:intergenic 40.0%
!! CCCCAACCATATGTTTTTAC+CGG + chr4_1:30220045-30220064 Msa0543650:CDS 40.0%
TATTTCGATAGCGTCTCTTC+TGG + chr4_1:30220187-30220206 Msa0543650:CDS 40.0%
GCTAGACCTTGATATCAAAC+AGG + chr4_1:30220315-30220334 Msa0543650:CDS 40.0%
TCTAGCTAACCATCTTCAGT+CGG + chr4_1:30220503-30220522 Msa0543650:intron 40.0%
TCTCAGGTTCAAATTTGGAG+AGG - chr4_1:30220533-30220552 None:intergenic 40.0%
! TACTGAGAGTGAAATGGACT+CGG - chr4_1:30220571-30220590 None:intergenic 40.0%
!! GGGCTTGTTGGAATGATATT+TGG - chr4_1:30220743-30220762 None:intergenic 40.0%
!! TTTCAGGGTTTTTAGGAGGA+TGG + chr4_1:30220818-30220837 Msa0543650:intron 40.0%
ACATCACAACAATGCCTTCT+TGG - chr4_1:30220898-30220917 None:intergenic 40.0%
CTCTAACAGCAATTTCCAGT+TGG - chr4_1:30220942-30220961 None:intergenic 40.0%
AAGTGAACCACCGTAATCCA+CGG - chr4_1:30218338-30218357 None:intergenic 45.0%
CATAGAGGAGGAAGAGAAGA+AGG - chr4_1:30218792-30218811 None:intergenic 45.0%
! TCGAAACCGTGAAGTGTCTT+CGG - chr4_1:30218820-30218839 None:intergenic 45.0%
GAGGACTTAGATCAGCTATC+CGG + chr4_1:30218853-30218872 Msa0543650:CDS 45.0%
AGGACTTAGATCAGCTATCC+GGG + chr4_1:30218854-30218873 Msa0543650:CDS 45.0%
TGTGTTTGGAGACGATGACA+CGG + chr4_1:30218932-30218951 Msa0543650:CDS 45.0%
!!! CGATGACACGGTTTTTTTCC+CGG + chr4_1:30218944-30218963 Msa0543650:CDS 45.0%
GAGTCTTCACCAAATTCTCC+GGG - chr4_1:30218965-30218984 None:intergenic 45.0%
!!! GGTTTTGGAATGGCTTTTGG+TGG + chr4_1:30219059-30219078 Msa0543650:CDS 45.0%
CCATCACTCCCATATAGATG+AGG - chr4_1:30219158-30219177 None:intergenic 45.0%
CCTCATCTATATGGGAGTGA+TGG + chr4_1:30219155-30219174 Msa0543650:CDS 45.0%
!! TGCTTAGCTGAACTTGGTGT+AGG + chr4_1:30219191-30219210 Msa0543650:CDS 45.0%
GTAGGATTGACACATGAACC+AGG + chr4_1:30219209-30219228 Msa0543650:CDS 45.0%
TGGATCCCACCAAATCATGT+GGG - chr4_1:30219467-30219486 None:intergenic 45.0%
ATGGATCCCACCAAATCATG+TGG - chr4_1:30219468-30219487 None:intergenic 45.0%
TTACTGGATGCGCAGCTAAT+AGG - chr4_1:30219832-30219851 None:intergenic 45.0%
AGAGACAGTAAGGGAGTTAC+TGG - chr4_1:30219848-30219867 None:intergenic 45.0%
GGATGATGCAGAGACAGTAA+GGG - chr4_1:30219857-30219876 None:intergenic 45.0%
TGGATGATGCAGAGACAGTA+AGG - chr4_1:30219858-30219877 None:intergenic 45.0%
TTACAAGGATCAGGGTGAAG+TGG - chr4_1:30220151-30220170 None:intergenic 45.0%
AGCGTCTCTTCTGGTAAAGA+TGG + chr4_1:30220196-30220215 Msa0543650:CDS 45.0%
AGTAGTCATCGACAAAGCTG+AGG - chr4_1:30220470-30220489 None:intergenic 45.0%
CTAGCTAACCATCTTCAGTC+GGG + chr4_1:30220504-30220523 Msa0543650:intron 45.0%
TGAACCTGAGATCTTGCTGA+AGG + chr4_1:30220542-30220561 Msa0543650:intron 45.0%
AACTTCGTGTTTGGGCTTGT+TGG - chr4_1:30220755-30220774 None:intergenic 45.0%
AGTTGGTCACTAGCACTTGA+GGG - chr4_1:30220925-30220944 None:intergenic 45.0%
!! GTTTGTTGTTTCTCACGCCG+TGG + chr4_1:30218318-30218337 Msa0543650:five_prime_UTR 50.0%
! GTTTCTCACGCCGTGGATTA+CGG + chr4_1:30218325-30218344 Msa0543650:five_prime_UTR 50.0%
TCTTCGGAGACACATAGAGG+AGG - chr4_1:30218804-30218823 None:intergenic 50.0%
GTGTCTTCGGAGACACATAG+AGG - chr4_1:30218807-30218826 None:intergenic 50.0%
GTGTCTCCGAAGACACTTCA+CGG + chr4_1:30218811-30218830 Msa0543650:CDS 50.0%
ACTTAGATCAGCTATCCGGG+TGG + chr4_1:30218857-30218876 Msa0543650:CDS 50.0%
! ATGGGACCCACATGATTTGG+TGG + chr4_1:30219458-30219477 Msa0543650:intron 50.0%
!! TGGGACCCACATGATTTGGT+GGG + chr4_1:30219459-30219478 Msa0543650:intron 50.0%
ATGCGCAGCTAATAGGCCAA+AGG - chr4_1:30219825-30219844 None:intergenic 50.0%
TCTCGCGCATTCACAACATC+CGG - chr4_1:30220067-30220086 None:intergenic 50.0%
CGCGAGAAACATTCAAGCAG+TGG + chr4_1:30220080-30220099 Msa0543650:CDS 50.0%
GATGCCTTCAGCAAGATCTC+AGG - chr4_1:30220549-30220568 None:intergenic 50.0%
TTCAGTTGCAGGCTCCAAGA+AGG + chr4_1:30220881-30220900 Msa0543650:intron 50.0%
CAGTTGGTCACTAGCACTTG+AGG - chr4_1:30220926-30220945 None:intergenic 50.0%
CTCAAGTGCTAGTGACCAAC+TGG + chr4_1:30220924-30220943 Msa0543650:CDS 50.0%
!!! GGAATGGCTTTTGGTGGTGC+TGG + chr4_1:30219065-30219084 Msa0543650:CDS 55.0%
AGAGGAGACCCGACTGAAGA+TGG - chr4_1:30220515-30220534 None:intergenic 55.0%
! TCTCACGCCGTGGATTACGG+TGG + chr4_1:30218328-30218347 Msa0543650:five_prime_UTR 60.0%
GCGTGTTGTCGCGGAGACTG+TGG + chr4_1:30218881-30218900 Msa0543650:CDS 65.0%
CCGCGACAACACGCGCCACC+CGG - chr4_1:30218875-30218894 None:intergenic 75.0%
CCGGGTGGCGCGTGTTGTCG+CGG + chr4_1:30218872-30218891 Msa0543650:CDS 75.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 30218161 30221169 30218161 ID=Msa0543650;Name=Msa0543650
chr4_1 mRNA 30218161 30221169 30218161 ID=Msa0543650-mRNA-1;Parent=Msa0543650;Name=Msa0543650-mRNA-1;_AED=0.02;_eAED=0.02;_QI=295|0.5|0.66|1|0.5|0.33|3|173|482
chr4_1 exon 30218161 30219241 30218161 ID=Msa0543650-mRNA-1:exon:8000;Parent=Msa0543650-mRNA-1
chr4_1 exon 30219803 30220354 30219803 ID=Msa0543650-mRNA-1:exon:8001;Parent=Msa0543650-mRNA-1
chr4_1 exon 30220886 30221169 30220886 ID=Msa0543650-mRNA-1:exon:8002;Parent=Msa0543650-mRNA-1
chr4_1 five_prime_UTR 30218161 30218455 30218161 ID=Msa0543650-mRNA-1:five_prime_utr;Parent=Msa0543650-mRNA-1
chr4_1 CDS 30218456 30219241 30218456 ID=Msa0543650-mRNA-1:cds;Parent=Msa0543650-mRNA-1
chr4_1 CDS 30219803 30220354 30219803 ID=Msa0543650-mRNA-1:cds;Parent=Msa0543650-mRNA-1
chr4_1 CDS 30220886 30220996 30220886 ID=Msa0543650-mRNA-1:cds;Parent=Msa0543650-mRNA-1
chr4_1 three_prime_UTR 30220997 30221169 30220997 ID=Msa0543650-mRNA-1:three_prime_utr;Parent=Msa0543650-mRNA-1
Gene Sequence

>Msa0543650

ATGCAGTTATTTCAGAAACACACAAAAGCACAATCTTTGATTAATTTCATATTAGTTACATCTTCAGTTTGTGGTTTTTATGTTCTTGTATCAGTACTAATATTAGGTACATCAAATTCAAAGCTATTAGTACATTTGCAACATTCTTCAGAATCATCAAATGATGTTTCCATAAAAAAAACAAACACAACTCTAGATGATATTGTGTTTGGAATTGCTTCAAGCAAGAGTTCATGGACAAAGAGAAAGGAGTATGTGAAACTATGGTGGAAAACCAACAATGCAATGAAGGGTTGTGTGTTCTTGGATAGTCTACCACAAAATGAATATGACCCTTCTTCTCTTCCTCCTCTATGTGTCTCCGAAGACACTTCACGGTTTCGATATACTTGCAAAGGAGGACTTAGATCAGCTATCCGGGTGGCGCGTGTTGTCGCGGAGACTGTGGCCTTGAATCATTCAAATGTGAAGTGGTATGTGTTTGGAGACGATGACACGGTTTTTTTCCCGGAGAATTTGGTGAAGACTCTTTCAAAATATGATCATGAGCTTTGGTATTATATTGGTGCACACTCTGAGATTTATGAGCAGAATCGGTTGTTTGGTTTTGGAATGGCTTTTGGTGGTGCTGGTTTTGCTATTAGTTCATCTCTTGCTAAGGTTTTGGCTAAGGTTTTTGATTCTTGTTTGGAAAGGTATCCTCATCTATATGGGAGTGATGGTAGGGTTTACTCTTGCTTAGCTGAACTTGGTGTAGGATTGACACATGAACCAGGTTTTCATCAGGTTGATTTAACTGGAAATACCTTTGGCCTATTAGCTGCGCATCCAGTAACTCCCTTACTGTCTCTGCATCATCCAGATTACACAGATCCAATCTTTCCAAACATGACTACTACACAAGCTCTAAAACATTTATTTGAAGCAGTAAATGTTGATTCTCAGAGGATGTTGCAACAAACAATTTGCTATGACAGATGGTTTTCATGGACAATTTCAGTGTCATGGGGTTATGCTGTTCAAGTATTCCCCAACCATATGTTTTTACCGGATGTTGTGAATGCGCGAGAAACATTCAAGCAGTGGAAAAAGGGAAATATGTTAGCAAAAGCTTATACTTTCAACACAAAACCACTTCACCCTGATCCTTGTAAAAGATCTACTATCTTCTATTTCGATAGCGTCTCTTCTGGTAAAGATGGTATTATAAACAGTTATTATAGGAGATCTTTTCAAAATTGCTCAAAGGATTTGGTGTCTCCAAAGAAACTAGAAGTCATCAAAGTGGTTACTCATAAGCTAGACCTTGATATCAAACAGGTAACTATTTGTGAATCCTTGCAGGCTCCAAGAAGGCATTGTTGTGATGTGTTGCCCTCAAGTGCTAGTGACCAACTGGAAATTGCTGTTAGAGAATGCAAAGATGAAGAATTGATTTACAAGCACTGA
Protein sequence

>Msa0543650

MQLFQKHTKAQSLINFILVTSSVCGFYVLVSVLILGTSNSKLLVHLQHSSESSNDVSIKKTNTTLDDIVFGIASSKSSWTKRKEYVKLWWKTNNAMKGCVFLDSLPQNEYDPSSLPPLCVSEDTSRFRYTCKGGLRSAIRVARVVAETVALNHSNVKWYVFGDDDTVFFPENLVKTLSKYDHELWYYIGAHSEIYEQNRLFGFGMAFGGAGFAISSSLAKVLAKVFDSCLERYPHLYGSDGRVYSCLAELGVGLTHEPGFHQVDLTGNTFGLLAAHPVTPLLSLHHPDYTDPIFPNMTTTQALKHLFEAVNVDSQRMLQQTICYDRWFSWTISVSWGYAVQVFPNHMFLPDVVNARETFKQWKKGNMLAKAYTFNTKPLHPDPCKRSTIFYFDSVSSGKDGIINSYYRRSFQNCSKDLVSPKKLEVIKVVTHKLDLDIKQVTICESLQAPRRHCCDVLPSSASDQLEIAVRECKDEELIYKH*