AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0547800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0547800 MtrunA17_Chr1g0179621 84.146 82 13 0 13 94 247 328 5.18e-45 151
Msa0547800 MtrunA17_Chr7g0265481 57.778 90 37 1 6 94 236 325 1.77e-31 115
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0547800 AT2G36630.1 56.881 109 47 0 13 121 241 349 2.90e-25 99.0

Find 79 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTAAGCTTTCCAATCTAAT+TGG 0.249520 4_1:-41245192 None:intergenic
TCAACTGTGTGCACAATTAT+AGG 0.259571 4_1:-41245131 None:intergenic
TTTGGAGGCTTACTTGGTTC+TGG 0.271593 4_1:+41243381 Msa0547800:CDS
TTGGGTGTTCTTCGGCTTGC+AGG 0.281027 4_1:+41243011 Msa0547800:CDS
TCTACATATTGGTAATAATT+AGG 0.298164 4_1:-41243431 None:intergenic
CCTTGATGATTTGAATTAAG+AGG 0.306448 4_1:-41242871 None:intergenic
TTACGAGTTACTATTGTTCA+TGG 0.315591 4_1:+41244618 Msa0547800:three_prime_UTR
CTATGATTCAACAACACAAT+AGG 0.324543 4_1:+41244164 Msa0547800:three_prime_UTR
AACCTTCCTCCAACTCTTCC+CGG 0.325188 4_1:-41244844 None:intergenic
TAAAGCAACCATGAATTTGT+AGG 0.325331 4_1:-41244786 None:intergenic
AGTTAGAATGGCAGCTTTGA+TGG 0.327425 4_1:+41244644 Msa0547800:three_prime_UTR
GGCTTACTTGGTTCTGGAGG+TGG 0.346407 4_1:+41243387 Msa0547800:CDS
CGGATGTGGTATTCTAAACT+TGG 0.350842 4_1:-41244824 None:intergenic
TGATGCCTTTGATGTTGAAT+TGG 0.359998 4_1:+41244881 Msa0547800:three_prime_UTR
CACTCAGAGAAGAACCTTGC+AGG 0.366434 4_1:+41243702 Msa0547800:three_prime_UTR
TGTGGATTGAGTGGTATTGT+AGG 0.389106 4_1:+41243351 Msa0547800:CDS
CAGAATTACATCTGAGAGTC+TGG 0.391894 4_1:-41243893 None:intergenic
CTGTGAAACCTACAAATTCA+TGG 0.395582 4_1:+41244778 Msa0547800:three_prime_UTR
TGTTGGTCTTGGACCCTTTG+AGG 0.406303 4_1:+41244692 Msa0547800:three_prime_UTR
GCAGGTTTCTTATCAAGATA+AGG 0.411591 4_1:+41244062 Msa0547800:intron
GTATGGTATTGGGTGTTCTT+CGG 0.414053 4_1:+41243003 Msa0547800:CDS
CAGGTTTCTTATCAAGATAA+GGG 0.416655 4_1:+41244063 Msa0547800:intron
CAAAAGCATCAACTGAGAAA+AGG 0.424023 4_1:-41242831 None:intergenic
GGTTGCGAAACCAATTAGAT+TGG 0.428571 4_1:+41245182 Msa0547800:three_prime_UTR
AATTATTACCAATATGTAGA+TGG 0.431548 4_1:+41243434 Msa0547800:exon
CACAAAATGAGGATGGGCAC+AGG 0.447354 4_1:+41243270 Msa0547800:CDS
ATACATATGTGAGGCATCAA+TGG 0.451588 4_1:+41243302 Msa0547800:CDS
TATGATTCAACAACACAATA+GGG 0.452908 4_1:+41244165 Msa0547800:three_prime_UTR
GCATATCTAGGTGTTGGTCT+TGG 0.455048 4_1:+41244681 Msa0547800:three_prime_UTR
CAAGTTTAGAATACCACATC+CGG 0.455397 4_1:+41244825 Msa0547800:three_prime_UTR
AAAAGCATCAACTGAGAAAA+GGG 0.456835 4_1:-41242830 None:intergenic
CCTCTTAATTCAAATCATCA+AGG 0.458016 4_1:+41242871 Msa0547800:CDS
GGTGCATACCATCTACATAT+TGG 0.458527 4_1:-41243442 None:intergenic
AGGACTGCAGTGTATGGTAT+TGG 0.459813 4_1:+41242992 Msa0547800:CDS
GATGCCTTTGATGTTGAATT+GGG 0.465301 4_1:+41244882 Msa0547800:three_prime_UTR
TCCACCTACAAGGCATATCT+AGG 0.471646 4_1:+41244669 Msa0547800:three_prime_UTR
CACCAGATTCATATTTATGC+AGG 0.472022 4_1:-41245160 None:intergenic
TGCCTGCATAAATATGAATC+TGG 0.475358 4_1:+41245158 Msa0547800:three_prime_UTR
TAGTTCTTAGCTACCTCAAA+GGG 0.489901 4_1:-41244705 None:intergenic
TTTGATGGCTTCCACCTACA+AGG 0.496451 4_1:+41244659 Msa0547800:three_prime_UTR
AACACCTAGATATGCCTTGT+AGG 0.502656 4_1:-41244673 None:intergenic
CCACATCCGGGAAGAGTTGG+AGG 0.506092 4_1:+41244838 Msa0547800:three_prime_UTR
CCTCCAACTCTTCCCGGATG+TGG 0.506607 4_1:-41244838 None:intergenic
TGTTCATGGAATAGTTAGAA+TGG 0.508282 4_1:+41244632 Msa0547800:three_prime_UTR
GGACTGCAGTGTATGGTATT+GGG 0.513027 4_1:+41242993 Msa0547800:CDS
CAACTGAGTTATACTCTCAA+GGG 0.530950 4_1:-41243641 None:intergenic
TCAACTGAGTTATACTCTCA+AGG 0.534430 4_1:-41243642 None:intergenic
TCCTGCTTTCAGCTACGAGA+GGG 0.539243 4_1:+41244587 Msa0547800:intron
TTAGTTCTTAGCTACCTCAA+AGG 0.542585 4_1:-41244706 None:intergenic
GGAGGCTTACTTGGTTCTGG+AGG 0.543501 4_1:+41243384 Msa0547800:CDS
CAAGGATCACAAAATGAGGA+TGG 0.545211 4_1:+41243263 Msa0547800:CDS
ATACCACATCCGGGAAGAGT+TGG 0.551523 4_1:+41244835 Msa0547800:three_prime_UTR
TGCACAAGGATCACAAAATG+AGG 0.557129 4_1:+41243259 Msa0547800:CDS
CAACATTGAGAGTGCAACCT+TGG 0.559014 4_1:+41244093 Msa0547800:three_prime_UTR
TTAACCCAATTCAACATCAA+AGG 0.561095 4_1:-41244886 None:intergenic
GAAAGCATGAATTATATCAC+AGG 0.570939 4_1:-41245053 None:intergenic
TATGTGAGGCATCAATGGAA+TGG 0.573024 4_1:+41243307 Msa0547800:CDS
TTCCTGCTTTCAGCTACGAG+AGG 0.576710 4_1:+41244586 Msa0547800:intron
ATGTAGAGGACTGCAGTGTA+TGG 0.579413 4_1:+41242986 Msa0547800:CDS
TTGGTAATAATTAGGAGAAG+AGG 0.581697 4_1:-41243423 None:intergenic
TACAAGGCATATCTAGGTGT+TGG 0.583608 4_1:+41244675 Msa0547800:three_prime_UTR
GGATTGAGTGGTATTGTAGG+TGG 0.589364 4_1:+41243354 Msa0547800:CDS
ATCCGGGAAGAGTTGGAGGA+AGG 0.590009 4_1:+41244842 Msa0547800:three_prime_UTR
ACTCAGAGAAGAACCTTGCA+GGG 0.591861 4_1:+41243703 Msa0547800:three_prime_UTR
ACCTAGATATGCCTTGTAGG+TGG 0.599115 4_1:-41244670 None:intergenic
CTTGGTGATGTTTGACAGAG+AGG 0.613489 4_1:+41244111 Msa0547800:three_prime_UTR
GATTATGAACAATAAAGTGA+AGG 0.615575 4_1:+41244743 Msa0547800:three_prime_UTR
ACAAAATGAGGATGGGCACA+GGG 0.615703 4_1:+41243271 Msa0547800:CDS
TGAAGGATTCAAGTTGCACA+AGG 0.622882 4_1:+41243245 Msa0547800:CDS
AAGGATCACAAAATGAGGAT+GGG 0.625681 4_1:+41243264 Msa0547800:CDS
TGGTTTGCAGACTGATGTAG+AGG 0.630937 4_1:+41242972 Msa0547800:intron
GAATGCTCAGTTAGAGCCTG+TGG 0.635783 4_1:+41243663 Msa0547800:three_prime_UTR
AACTGAGTTATACTCTCAAG+GGG 0.642471 4_1:-41243640 None:intergenic
GTAGTCGTTAAGACCCTGCA+AGG 0.651824 4_1:-41243716 None:intergenic
AAGTTTAGAATACCACATCC+GGG 0.653715 4_1:+41244826 Msa0547800:three_prime_UTR
GAGCCTAAAATACATATGTG+AGG 0.661800 4_1:+41243293 Msa0547800:CDS
CTCTCTGTCAAACATCACCA+AGG 0.681370 4_1:-41244110 None:intergenic
CTGCATAAATATGAATCTGG+TGG 0.709679 4_1:+41245161 Msa0547800:three_prime_UTR
ACTGAGTTATACTCTCAAGG+GGG 0.767520 4_1:-41243639 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATATTTTAATTTCCAAAT+CGG + chr4_1:41244505-41244524 Msa0547800:intron 10.0%
!!! CATTTATAGAGTTTTAAAAA+AGG - chr4_1:41243143-41243162 None:intergenic 15.0%
!!! CAAGTCTTATATTTTTATAT+TGG + chr4_1:41245234-41245253 Msa0547800:three_prime_UTR 15.0%
!! TAAAATAAGCAGTTTATTGT+TGG + chr4_1:41242952-41242971 Msa0547800:intron 20.0%
!!! TCTACATATTGGTAATAATT+AGG - chr4_1:41243434-41243453 None:intergenic 20.0%
!! AATTATTACCAATATGTAGA+TGG + chr4_1:41243434-41243453 Msa0547800:exon 20.0%
!!! TATAGTGTTTTTTTTATGCT+AGG + chr4_1:41243591-41243610 Msa0547800:intron 20.0%
!!! AATCATGATTTTTCTTAGTT+TGG - chr4_1:41243966-41243985 None:intergenic 20.0%
!!! CTAAGAAAAATCATGATTTT+AGG + chr4_1:41243968-41243987 Msa0547800:intron 20.0%
!!! AATTTTTCAGAAGTAATAGA+TGG - chr4_1:41244260-41244279 None:intergenic 20.0%
!!! TCAACTTTTTCAAAATACAA+AGG - chr4_1:41244915-41244934 None:intergenic 20.0%
! AAAACTCTATAAATGCAATG+TGG + chr4_1:41243148-41243167 Msa0547800:intron 25.0%
! TTGGAAACTGAAACAAAAAA+TGG - chr4_1:41243203-41243222 None:intergenic 25.0%
! TTCAATAAAAATTTCTCCAC+AGG - chr4_1:41243682-41243701 None:intergenic 25.0%
!! ACAATCAACTAGCTTATATT+TGG + chr4_1:41243807-41243826 Msa0547800:intron 25.0%
!!! GAATTTTGAACTCTGAAAAT+TGG + chr4_1:41243834-41243853 Msa0547800:intron 25.0%
!! CTTTTTATAATCCATTTTGC+AGG + chr4_1:41244044-41244063 Msa0547800:intron 25.0%
! TATGATTCAACAACACAATA+GGG + chr4_1:41244165-41244184 Msa0547800:three_prime_UTR 25.0%
!! AAATTTTGTAACATCCTCAA+TGG - chr4_1:41244467-41244486 None:intergenic 25.0%
! AATTTCCAAATCGGTTAATT+TGG + chr4_1:41244514-41244533 Msa0547800:intron 25.0%
! TATAACCAAATTAACCGATT+TGG - chr4_1:41244522-41244541 None:intergenic 25.0%
! GATTATGAACAATAAAGTGA+AGG + chr4_1:41244743-41244762 Msa0547800:three_prime_UTR 25.0%
!! AAAAGCATCAACTGAGAAAA+GGG - chr4_1:41242833-41242852 None:intergenic 30.0%
! CCTTGATGATTTGAATTAAG+AGG - chr4_1:41242874-41242893 None:intergenic 30.0%
CCTCTTAATTCAAATCATCA+AGG + chr4_1:41242871-41242890 Msa0547800:CDS 30.0%
ACTGCGATTCATAATATGTT+TGG - chr4_1:41243058-41243077 None:intergenic 30.0%
CATATTATGAATCGCAGTAA+TGG + chr4_1:41243060-41243079 Msa0547800:intron 30.0%
ACAAAAAATGGACATGTGTA+TGG - chr4_1:41243191-41243210 None:intergenic 30.0%
! TCAGTTTCCAATTGCATTTT+TGG + chr4_1:41243212-41243231 Msa0547800:intron 30.0%
! ATGCCTCACATATGTATTTT+AGG - chr4_1:41243299-41243318 None:intergenic 30.0%
! TTGGTAATAATTAGGAGAAG+AGG - chr4_1:41243426-41243445 None:intergenic 30.0%
!! ATGTCACTAAAGTGTTTTCA+AGG + chr4_1:41243933-41243952 Msa0547800:three_prime_UTR 30.0%
CCTCAACAATATAATCATGT+TGG - chr4_1:41244017-41244036 None:intergenic 30.0%
CCAACATGATTATATTGTTG+AGG + chr4_1:41244014-41244033 Msa0547800:intron 30.0%
TTGATAAGAAACCTGCAAAA+TGG - chr4_1:41244058-41244077 None:intergenic 30.0%
CAGGTTTCTTATCAAGATAA+GGG + chr4_1:41244063-41244082 Msa0547800:intron 30.0%
CTATGATTCAACAACACAAT+AGG + chr4_1:41244164-41244183 Msa0547800:three_prime_UTR 30.0%
!! CTGGTTCTAATCAAATTTGA+AGG - chr4_1:41244204-41244223 None:intergenic 30.0%
! AATATGAAATGGGACTTTTC+TGG - chr4_1:41244223-41244242 None:intergenic 30.0%
!! TGTACTTTGCTGTCAAATTT+TGG + chr4_1:41244303-41244322 Msa0547800:intron 30.0%
!! AAATTTTGGTATCTTGGTCA+GGG + chr4_1:41244317-41244336 Msa0547800:intron 30.0%
TTGCACAAACTTTCAACTTA+GGG - chr4_1:41244362-41244381 None:intergenic 30.0%
TTTGCACAAACTTTCAACTT+AGG - chr4_1:41244363-41244382 None:intergenic 30.0%
TTACGAGTTACTATTGTTCA+TGG + chr4_1:41244618-41244637 Msa0547800:three_prime_UTR 30.0%
TGTTCATGGAATAGTTAGAA+TGG + chr4_1:41244632-41244651 Msa0547800:three_prime_UTR 30.0%
TAAAGCAACCATGAATTTGT+AGG - chr4_1:41244789-41244808 None:intergenic 30.0%
TTAACCCAATTCAACATCAA+AGG - chr4_1:41244889-41244908 None:intergenic 30.0%
! GAAAGCATGAATTATATCAC+AGG - chr4_1:41245056-41245075 None:intergenic 30.0%
AGTAAGCTTTCCAATCTAAT+TGG - chr4_1:41245195-41245214 None:intergenic 30.0%
!! CAAAAGCATCAACTGAGAAA+AGG - chr4_1:41242834-41242853 None:intergenic 35.0%
!! AGTTGATGCTTTTGTCTGTT+TGG + chr4_1:41242840-41242859 Msa0547800:CDS 35.0%
ATCAAGAATCATGATGAGAC+TGG - chr4_1:41243096-41243115 None:intergenic 35.0%
CTTGATATTGCAAAGAATCG+CGG + chr4_1:41243110-41243129 Msa0547800:intron 35.0%
CCAAACACCAAAAATGCAAT+TGG - chr4_1:41243222-41243241 None:intergenic 35.0%
!!! CCAATTGCATTTTTGGTGTT+TGG + chr4_1:41243219-41243238 Msa0547800:CDS 35.0%
!!! TTTTTGGTGTTTGGCTTTGA+AGG + chr4_1:41243228-41243247 Msa0547800:CDS 35.0%
AAGGATCACAAAATGAGGAT+GGG + chr4_1:41243264-41243283 Msa0547800:CDS 35.0%
GAGCCTAAAATACATATGTG+AGG + chr4_1:41243293-41243312 Msa0547800:CDS 35.0%
! ATACATATGTGAGGCATCAA+TGG + chr4_1:41243302-41243321 Msa0547800:CDS 35.0%
! GGTATTGTAGGTGGAATTTT+TGG + chr4_1:41243363-41243382 Msa0547800:CDS 35.0%
!! ATTGTAGGTGGAATTTTTGG+AGG + chr4_1:41243366-41243385 Msa0547800:CDS 35.0%
AACTGAGTTATACTCTCAAG+GGG - chr4_1:41243643-41243662 None:intergenic 35.0%
CAACTGAGTTATACTCTCAA+GGG - chr4_1:41243644-41243663 None:intergenic 35.0%
TCAACTGAGTTATACTCTCA+AGG - chr4_1:41243645-41243664 None:intergenic 35.0%
GCAGGTTTCTTATCAAGATA+AGG + chr4_1:41244062-41244081 Msa0547800:intron 35.0%
CCTGAAAGGGAATATGAAAT+GGG - chr4_1:41244233-41244252 None:intergenic 35.0%
TCCTGAAAGGGAATATGAAA+TGG - chr4_1:41244234-41244253 None:intergenic 35.0%
AATAGATGGAGTTCCTGAAA+GGG - chr4_1:41244246-41244265 None:intergenic 35.0%
TAATAGATGGAGTTCCTGAA+AGG - chr4_1:41244247-41244266 None:intergenic 35.0%
!! GCTGTCAAATTTTGGTATCT+TGG + chr4_1:41244311-41244330 Msa0547800:intron 35.0%
!! CAAATTTTGGTATCTTGGTC+AGG + chr4_1:41244316-41244335 Msa0547800:intron 35.0%
TCAATGGCATCAAACACAAA+AGG - chr4_1:41244451-41244470 None:intergenic 35.0%
! TTTTGTGTTTGATGCCATTG+AGG + chr4_1:41244450-41244469 Msa0547800:intron 35.0%
TAGTTCTTAGCTACCTCAAA+GGG - chr4_1:41244708-41244727 None:intergenic 35.0%
! TTAGTTCTTAGCTACCTCAA+AGG - chr4_1:41244709-41244728 None:intergenic 35.0%
CTGTGAAACCTACAAATTCA+TGG + chr4_1:41244778-41244797 Msa0547800:three_prime_UTR 35.0%
! CAAGTTTAGAATACCACATC+CGG + chr4_1:41244825-41244844 Msa0547800:three_prime_UTR 35.0%
! AAGTTTAGAATACCACATCC+GGG + chr4_1:41244826-41244845 Msa0547800:three_prime_UTR 35.0%
TGATGCCTTTGATGTTGAAT+TGG + chr4_1:41244881-41244900 Msa0547800:three_prime_UTR 35.0%
GATGCCTTTGATGTTGAATT+GGG + chr4_1:41244882-41244901 Msa0547800:three_prime_UTR 35.0%
TCAACTGTGTGCACAATTAT+AGG - chr4_1:41245134-41245153 None:intergenic 35.0%
TGCCTGCATAAATATGAATC+TGG + chr4_1:41245158-41245177 Msa0547800:three_prime_UTR 35.0%
CACCAGATTCATATTTATGC+AGG - chr4_1:41245163-41245182 None:intergenic 35.0%
CTGCATAAATATGAATCTGG+TGG + chr4_1:41245161-41245180 Msa0547800:three_prime_UTR 35.0%
AGAACTCTTAGTGAGTGAGA+GGG - chr4_1:41242904-41242923 None:intergenic 40.0%
TAGAACTCTTAGTGAGTGAG+AGG - chr4_1:41242905-41242924 None:intergenic 40.0%
! GTATGGTATTGGGTGTTCTT+CGG + chr4_1:41243003-41243022 Msa0547800:CDS 40.0%
!! TGAAGGATTCAAGTTGCACA+AGG + chr4_1:41243245-41243264 Msa0547800:CDS 40.0%
TGCACAAGGATCACAAAATG+AGG + chr4_1:41243259-41243278 Msa0547800:CDS 40.0%
CAAGGATCACAAAATGAGGA+TGG + chr4_1:41243263-41243282 Msa0547800:CDS 40.0%
! TATGTGAGGCATCAATGGAA+TGG + chr4_1:41243307-41243326 Msa0547800:CDS 40.0%
! GCTACATCACTTGCATTTTG+TGG + chr4_1:41243333-41243352 Msa0547800:CDS 40.0%
!! CTTGCATTTTGTGGATTGAG+TGG + chr4_1:41243342-41243361 Msa0547800:CDS 40.0%
! TGTGGATTGAGTGGTATTGT+AGG + chr4_1:41243351-41243370 Msa0547800:CDS 40.0%
!! GGAATTTTTGGAGGCTTACT+TGG + chr4_1:41243375-41243394 Msa0547800:CDS 40.0%
GGTGCATACCATCTACATAT+TGG - chr4_1:41243445-41243464 None:intergenic 40.0%
TGTGGGATTGAAAACCAAGA+GGG - chr4_1:41243466-41243485 None:intergenic 40.0%
ATGTGGGATTGAAAACCAAG+AGG - chr4_1:41243467-41243486 None:intergenic 40.0%
TTCAATCCCACATGTGTTGA+TGG + chr4_1:41243474-41243493 Msa0547800:intron 40.0%
ACTGAGTTATACTCTCAAGG+GGG - chr4_1:41243642-41243661 None:intergenic 40.0%
CAGAATTACATCTGAGAGTC+TGG - chr4_1:41243896-41243915 None:intergenic 40.0%
!! AGCGCATGTTTTCTGGAAAA+GGG - chr4_1:41244143-41244162 None:intergenic 40.0%
!! TAGCGCATGTTTTCTGGAAA+AGG - chr4_1:41244144-41244163 None:intergenic 40.0%
! TCATAGTAGCGCATGTTTTC+TGG - chr4_1:41244150-41244169 None:intergenic 40.0%
CCCATTTCATATTCCCTTTC+AGG + chr4_1:41244230-41244249 Msa0547800:intron 40.0%
! CGTTTAGCTTCATTCTTCAG+TGG + chr4_1:41244388-41244407 Msa0547800:intron 40.0%
CACAAAAGGCAATAGATGAG+TGG - chr4_1:41244437-41244456 None:intergenic 40.0%
! AGTTAGAATGGCAGCTTTGA+TGG + chr4_1:41244644-41244663 Msa0547800:three_prime_UTR 40.0%
AACACCTAGATATGCCTTGT+AGG - chr4_1:41244676-41244695 None:intergenic 40.0%
TACAAGGCATATCTAGGTGT+TGG + chr4_1:41244675-41244694 Msa0547800:three_prime_UTR 40.0%
CGGATGTGGTATTCTAAACT+TGG - chr4_1:41244827-41244846 None:intergenic 40.0%
GGTTGCGAAACCAATTAGAT+TGG + chr4_1:41245182-41245201 Msa0547800:three_prime_UTR 40.0%
! TGGTTTGCAGACTGATGTAG+AGG + chr4_1:41242972-41242991 Msa0547800:intron 45.0%
ATGTAGAGGACTGCAGTGTA+TGG + chr4_1:41242986-41243005 Msa0547800:CDS 45.0%
AGGACTGCAGTGTATGGTAT+TGG + chr4_1:41242992-41243011 Msa0547800:CDS 45.0%
GGACTGCAGTGTATGGTATT+GGG + chr4_1:41242993-41243012 Msa0547800:CDS 45.0%
ATGAATCGCAGTAATGGTCG+CGG + chr4_1:41243066-41243085 Msa0547800:intron 45.0%
ACAAAATGAGGATGGGCACA+GGG + chr4_1:41243271-41243290 Msa0547800:CDS 45.0%
GGATTGAGTGGTATTGTAGG+TGG + chr4_1:41243354-41243373 Msa0547800:CDS 45.0%
TTTGGAGGCTTACTTGGTTC+TGG + chr4_1:41243381-41243400 Msa0547800:CDS 45.0%
GCAACACCATCAACACATGT+GGG - chr4_1:41243483-41243502 None:intergenic 45.0%
AGCAACACCATCAACACATG+TGG - chr4_1:41243484-41243503 None:intergenic 45.0%
AGTTATACTCTCAAGGGGGA+AGG - chr4_1:41243638-41243657 None:intergenic 45.0%
ACTCAGAGAAGAACCTTGCA+GGG + chr4_1:41243703-41243722 Msa0547800:three_prime_UTR 45.0%
! CAACATTGAGAGTGCAACCT+TGG + chr4_1:41244093-41244112 Msa0547800:three_prime_UTR 45.0%
CTCTCTGTCAAACATCACCA+AGG - chr4_1:41244113-41244132 None:intergenic 45.0%
!! CTTGGTGATGTTTGACAGAG+AGG + chr4_1:41244111-41244130 Msa0547800:three_prime_UTR 45.0%
! TTTGATGGCTTCCACCTACA+AGG + chr4_1:41244659-41244678 Msa0547800:three_prime_UTR 45.0%
TCCACCTACAAGGCATATCT+AGG + chr4_1:41244669-41244688 Msa0547800:three_prime_UTR 45.0%
ACCTAGATATGCCTTGTAGG+TGG - chr4_1:41244673-41244692 None:intergenic 45.0%
!! GCATATCTAGGTGTTGGTCT+TGG + chr4_1:41244681-41244700 Msa0547800:three_prime_UTR 45.0%
!! TTTAATAGAAAAAAATTATT+TGG + chr4_1:41244278-41244297 Msa0547800:intron 5.0%
CACAAAATGAGGATGGGCAC+AGG + chr4_1:41243270-41243289 Msa0547800:CDS 50.0%
GTAGATGGTATGCACCCTCT+TGG + chr4_1:41243449-41243468 Msa0547800:intron 50.0%
!! GTGTTGATGGTGTTGCTACG+AGG + chr4_1:41243487-41243506 Msa0547800:intron 50.0%
GAATGCTCAGTTAGAGCCTG+TGG + chr4_1:41243663-41243682 Msa0547800:three_prime_UTR 50.0%
CACTCAGAGAAGAACCTTGC+AGG + chr4_1:41243702-41243721 Msa0547800:three_prime_UTR 50.0%
GTAGTCGTTAAGACCCTGCA+AGG - chr4_1:41243719-41243738 None:intergenic 50.0%
TTCCTGCTTTCAGCTACGAG+AGG + chr4_1:41244586-41244605 Msa0547800:intron 50.0%
TCCTGCTTTCAGCTACGAGA+GGG + chr4_1:41244587-41244606 Msa0547800:intron 50.0%
!! TGTTGGTCTTGGACCCTTTG+AGG + chr4_1:41244692-41244711 Msa0547800:three_prime_UTR 50.0%
ATACCACATCCGGGAAGAGT+TGG + chr4_1:41244835-41244854 Msa0547800:three_prime_UTR 50.0%
AACCTTCCTCCAACTCTTCC+CGG - chr4_1:41244847-41244866 None:intergenic 50.0%
! TTGGGTGTTCTTCGGCTTGC+AGG + chr4_1:41243011-41243030 Msa0547800:CDS 55.0%
!! GGAGGCTTACTTGGTTCTGG+AGG + chr4_1:41243384-41243403 Msa0547800:CDS 55.0%
!! GGCTTACTTGGTTCTGGAGG+TGG + chr4_1:41243387-41243406 Msa0547800:CDS 55.0%
TCCCTCTCGTAGCTGAAAGC+AGG - chr4_1:41244591-41244610 None:intergenic 55.0%
ATCCGGGAAGAGTTGGAGGA+AGG + chr4_1:41244842-41244861 Msa0547800:three_prime_UTR 55.0%
CCTCCAACTCTTCCCGGATG+TGG - chr4_1:41244841-41244860 None:intergenic 60.0%
CCACATCCGGGAAGAGTTGG+AGG + chr4_1:41244838-41244857 Msa0547800:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 41242806 41245283 41242806 ID=Msa0547800;Name=Msa0547800
chr4_1 mRNA 41242806 41245283 41242806 ID=Msa0547800-mRNA-1;Parent=Msa0547800;Name=Msa0547800-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|1|0.85|1|0.33|0.57|7|1016|124
chr4_1 exon 41242806 41242892 41242806 ID=Msa0547800-mRNA-1:exon:10581;Parent=Msa0547800-mRNA-1
chr4_1 exon 41242982 41243032 41242982 ID=Msa0547800-mRNA-1:exon:10582;Parent=Msa0547800-mRNA-1
chr4_1 exon 41243216 41243455 41243216 ID=Msa0547800-mRNA-1:exon:10583;Parent=Msa0547800-mRNA-1
chr4_1 exon 41243613 41243744 41243613 ID=Msa0547800-mRNA-1:exon:10584;Parent=Msa0547800-mRNA-1
chr4_1 exon 41243880 41243954 41243880 ID=Msa0547800-mRNA-1:exon:10585;Parent=Msa0547800-mRNA-1
chr4_1 exon 41244066 41244186 41244066 ID=Msa0547800-mRNA-1:exon:10586;Parent=Msa0547800-mRNA-1
chr4_1 exon 41244599 41245283 41244599 ID=Msa0547800-mRNA-1:exon:10587;Parent=Msa0547800-mRNA-1
chr4_1 CDS 41242806 41242892 41242806 ID=Msa0547800-mRNA-1:cds;Parent=Msa0547800-mRNA-1
chr4_1 CDS 41242982 41243032 41242982 ID=Msa0547800-mRNA-1:cds;Parent=Msa0547800-mRNA-1
chr4_1 CDS 41243216 41243452 41243216 ID=Msa0547800-mRNA-1:cds;Parent=Msa0547800-mRNA-1
chr4_1 three_prime_UTR 41243453 41243455 41243453 ID=Msa0547800-mRNA-1:three_prime_utr;Parent=Msa0547800-mRNA-1
chr4_1 three_prime_UTR 41243613 41243744 41243613 ID=Msa0547800-mRNA-1:three_prime_utr;Parent=Msa0547800-mRNA-1
chr4_1 three_prime_UTR 41243880 41243954 41243880 ID=Msa0547800-mRNA-1:three_prime_utr;Parent=Msa0547800-mRNA-1
chr4_1 three_prime_UTR 41244066 41244186 41244066 ID=Msa0547800-mRNA-1:three_prime_utr;Parent=Msa0547800-mRNA-1
chr4_1 three_prime_UTR 41244599 41245283 41244599 ID=Msa0547800-mRNA-1:three_prime_utr;Parent=Msa0547800-mRNA-1
Gene Sequence

>Msa0547800

ATGTTCAATTCAAACTCTAATATTCCCTTTTCTCAGTTGATGCTTTTGTCTGTTTGGCTTTCTTTCCTCTTAATTCAAATCATCAAGACTGATGTAGAGGACTGCAGTGTATGGTATTGGGTGTTCTTCGGCTTGCAGTTTCCAATTGCATTTTTGGTGTTTGGCTTTGAAGGATTCAAGTTGCACAAGGATCACAAAATGAGGATGGGCACAGGGAGCCTAAAATACATATGTGAGGCATCAATGGAATGGAGTGCTACATCACTTGCATTTTGTGGATTGAGTGGTATTGTAGGTGGAATTTTTGGAGGCTTACTTGGTTCTGGAGGTGGATTTATTTTCAGACCTCTTCTCCTAATTATTACCAATATGTAG
Protein sequence

>Msa0547800

MFNSNSNIPFSQLMLLSVWLSFLLIQIIKTDVEDCSVWYWVFFGLQFPIAFLVFGFEGFKLHKDHKMRMGTGSLKYICEASMEWSATSLAFCGLSGIVGGIFGGLLGSGGGFIFRPLLLIITNM*