AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0548530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0548530 MtrunA17_Chr4g0031741 96.494 656 15 2 1 652 1 652 0.0 1303
Msa0548530 MtrunA17_Chr2g0328411 59.355 620 207 13 65 650 46 654 0.0 706
Msa0548530 MtrunA17_Chr2g0328431 54.440 687 247 13 1 652 1 656 0.0 702
Msa0548530 MtrunA17_Chr2g0328401 58.375 603 201 12 66 631 47 636 0.0 675
Msa0548530 MtrunA17_Chr7g0238891 43.909 747 308 17 8 652 5 742 0.0 572
Msa0548530 MtrunA17_Chr4g0031721 56.303 476 185 9 187 652 224 686 1.29e-180 528
Msa0548530 MtrunA17_Chr4g0031701 46.825 630 268 16 63 652 26 628 2.21e-177 518
Msa0548530 MtrunA17_Chr4g0031711 53.877 503 206 12 163 652 246 735 3.60e-177 521
Msa0548530 MtrunA17_Chr2g0328391 55.076 463 183 9 202 652 222 671 8.85e-174 510
Msa0548530 MtrunA17_Chr4g0031731 46.032 630 273 15 63 652 26 628 2.03e-173 508
Msa0548530 MtrunA17_Chr2g0328341 49.318 440 202 6 218 652 217 640 4.25e-148 443
Msa0548530 MtrunA17_Chr2g0328381 41.368 614 309 15 70 652 50 643 1.70e-147 442
Msa0548530 MtrunA17_Chr2g0328361 45.952 457 199 10 203 652 202 617 3.77e-131 399
Msa0548530 MtrunA17_Chr2g0328371 52.941 255 116 1 351 605 1 251 1.08e-96 297
Msa0548530 MtrunA17_Chr2g0290241 31.630 411 271 6 243 649 20 424 4.85e-65 220
Msa0548530 MtrunA17_Chr5g0447691 33.245 376 246 4 277 649 170 543 1.47e-64 222
Msa0548530 MtrunA17_Chr2g0319091 32.708 373 246 4 280 649 208 578 7.54e-61 213
Msa0548530 MtrunA17_Chr1g0159461 33.333 369 241 4 284 649 225 591 1.24e-60 213
Msa0548530 MtrunA17_Chr3g0125811 31.707 369 247 4 284 649 203 569 6.26e-58 205
Msa0548530 MtrunA17_Chr4g0076091 32.626 377 246 6 279 650 170 543 5.76e-57 201
Msa0548530 MtrunA17_Chr4g0057891 32.642 386 231 9 281 649 146 519 7.76e-57 201
Msa0548530 MtrunA17_Chr3g0101141 31.510 384 254 7 271 649 162 541 2.53e-56 200
Msa0548530 MtrunA17_Chr5g0445331 30.769 377 252 5 280 649 157 531 9.17e-56 198
Msa0548530 MtrunA17_Chr7g0224271 31.202 391 236 6 279 649 295 672 2.81e-55 199
Msa0548530 MtrunA17_Chr8g0376381 32.345 371 226 9 285 649 170 521 2.32e-50 183
Msa0548530 MtrunA17_Chr7g0245601 31.436 369 233 8 287 651 441 793 2.68e-48 181
Msa0548530 MtrunA17_Chr8g0357601 29.974 387 249 6 279 652 177 554 5.80e-48 177
Msa0548530 MtrunA17_Chr3g0110971 30.711 394 233 10 277 649 212 586 2.33e-47 176
Msa0548530 MtrunA17_Chr2g0306641 32.115 383 236 12 277 649 118 486 3.41e-46 171
Msa0548530 MtrunA17_Chr4g0038851 27.378 431 244 8 281 649 45 468 2.85e-45 167
Msa0548530 MtrunA17_Chr3g0085681 27.990 418 240 8 285 649 26 435 1.93e-41 156
Msa0548530 MtrunA17_Chr4g0057291 25.455 385 265 8 280 651 288 663 3.51e-40 156
Msa0548530 MtrunA17_Chr1g0200401 30.273 403 236 13 276 649 72 458 5.80e-40 152
Msa0548530 MtrunA17_Chr2g0294461 28.941 387 252 8 279 649 205 584 1.11e-39 154
Msa0548530 MtrunA17_Chr4g0038761 29.947 374 238 9 287 652 87 444 1.76e-39 150
Msa0548530 MtrunA17_Chr5g0401131 27.979 386 252 9 284 651 114 491 2.34e-36 142
Msa0548530 MtrunA17_Chr4g0039661 27.807 374 248 9 285 649 194 554 8.28e-36 142
Msa0548530 MtrunA17_Chr2g0294471 26.753 385 260 9 279 649 199 575 1.21e-34 139
Msa0548530 MtrunA17_Chr1g0183641 27.857 420 263 15 243 650 86 477 4.35e-34 136
Msa0548530 MtrunA17_Chr7g0270031 27.273 385 251 10 279 649 184 553 1.20e-33 135
Msa0548530 MtrunA17_Chr7g0236851 29.655 290 190 6 366 649 375 656 1.93e-31 130
Msa0548530 MtrunA17_Chr3g0114841 28.193 415 238 18 270 650 112 500 2.23e-30 125
Msa0548530 MtrunA17_Chr1g0194621 24.566 403 268 11 268 649 130 517 1.52e-29 123
Msa0548530 MtrunA17_Chr5g0424131 27.123 424 259 16 258 650 95 499 1.53e-28 120
Msa0548530 MtrunA17_Chr5g0396491 30.645 248 153 5 252 492 30 265 5.27e-28 118
Msa0548530 MtrunA17_Chr5g0396491 30.000 150 104 1 500 649 329 477 8.13e-17 83.6
Msa0548530 MtrunA17_Chr6g0468561 26.385 379 252 10 285 649 296 661 7.76e-28 119
Msa0548530 MtrunA17_Chr8g0383981 27.229 415 267 16 252 652 105 498 6.52e-27 115
Msa0548530 MtrunA17_Chr2g0329591 26.923 390 252 11 284 651 74 452 1.97e-26 112
Msa0548530 MtrunA17_Chr4g0053901 25.180 417 259 11 284 652 59 470 9.36e-25 108
Msa0548530 MtrunA17_Chr3g0085361 25.852 352 203 7 347 649 7 349 1.20e-24 105
Msa0548530 MtrunA17_Chr5g0401421 23.923 418 265 12 284 652 35 448 1.75e-24 107
Msa0548530 MtrunA17_Chr7g0230731 24.138 406 267 9 261 649 346 727 2.99e-19 92.4
Msa0548530 MtrunA17_Chr7g0230771 24.051 395 281 8 261 649 349 730 5.42e-19 91.7
Msa0548530 MtrunA17_Chr4g0012071 26.633 199 138 3 261 457 175 367 4.53e-16 81.6
Msa0548530 MtrunA17_Chr2g0323111 27.778 180 126 4 474 652 132 308 2.53e-13 71.6
Msa0548530 MtrunA17_Chr8g0344101 22.799 443 284 17 245 649 177 599 1.26e-12 70.9
Msa0548530 MtrunA17_Chr8g0373561 28.571 147 98 4 282 421 177 323 2.55e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0548530 AT2G29060.2 44.131 673 320 19 11 651 8 656 2.08e-179 525
Msa0548530 AT1G07530.1 55.973 452 190 6 208 652 318 767 4.21e-177 523
Msa0548530 AT5G59450.1 44.640 625 273 15 39 652 38 600 1.38e-172 506
Msa0548530 AT3G46600.1 46.755 601 248 10 59 652 46 581 1.64e-171 502
Msa0548530 AT2G29065.1 45.691 615 284 14 70 652 34 630 1.97e-166 491
Msa0548530 AT2G29060.1 52.298 457 207 8 203 651 240 693 1.22e-161 481
Msa0548530 AT2G29060.1 39.216 102 51 3 11 112 8 98 7.69e-12 68.9
Msa0548530 AT3G46600.3 52.980 453 189 5 205 652 116 549 2.03e-160 472
Msa0548530 AT2G37650.1 51.844 461 205 6 205 651 257 714 3.83e-159 475
Msa0548530 AT2G37650.2 51.844 461 205 6 205 651 245 702 5.80e-159 474
Msa0548530 AT3G46600.2 58.221 371 152 2 284 652 4 373 1.44e-155 453
Msa0548530 AT1G07520.2 51.648 455 195 10 208 652 92 531 6.44e-153 452
Msa0548530 AT1G07520.4 51.648 455 195 10 208 652 92 531 6.44e-153 452
Msa0548530 AT1G07520.3 51.648 455 195 10 208 652 257 696 3.42e-151 454
Msa0548530 AT1G07520.1 51.648 455 195 10 208 652 256 695 3.62e-151 454
Msa0548530 AT1G50600.3 34.234 444 269 9 213 649 98 525 2.41e-67 229
Msa0548530 AT1G50600.1 34.234 444 269 9 213 649 169 596 9.51e-67 230
Msa0548530 AT1G50600.2 34.234 444 269 9 213 649 173 600 1.13e-66 229
Msa0548530 AT5G48150.1 31.072 457 284 8 210 649 47 489 9.32e-63 216
Msa0548530 AT5G48150.4 31.072 457 284 8 210 649 47 489 9.32e-63 216
Msa0548530 AT5G48150.2 31.072 457 284 8 210 649 47 489 9.32e-63 216
Msa0548530 AT5G48150.3 31.072 457 284 8 210 649 47 489 9.32e-63 216
Msa0548530 AT4G17230.1 33.158 380 249 4 275 651 149 526 4.13e-57 202
Msa0548530 AT4G17230.2 33.158 380 249 4 275 651 149 526 4.13e-57 202
Msa0548530 AT2G04890.1 31.903 373 242 5 280 649 49 412 5.77e-57 199
Msa0548530 AT1G21450.1 31.806 371 245 6 284 649 225 592 1.05e-54 197
Msa0548530 AT3G54220.1 32.979 376 221 12 287 651 296 651 3.63e-49 182
Msa0548530 AT3G03450.1 31.620 389 230 12 277 649 176 544 1.53e-47 176
Msa0548530 AT1G66350.1 30.561 481 268 17 198 649 62 505 3.85e-47 174
Msa0548530 AT1G14920.1 31.217 378 237 9 279 649 167 528 2.21e-45 169
Msa0548530 AT5G17490.1 30.491 387 231 12 277 649 153 515 2.43e-45 169
Msa0548530 AT2G01570.1 31.771 384 227 12 279 649 219 580 5.40e-45 169
Msa0548530 AT5G66770.1 31.608 367 239 9 288 649 224 583 1.30e-43 166
Msa0548530 AT3G50650.1 30.618 356 232 7 302 649 193 541 5.16e-41 157
Msa0548530 AT4G37650.1 27.708 397 248 11 283 651 145 530 1.11e-39 153
Msa0548530 AT5G41920.1 27.083 384 251 9 276 649 35 399 3.54e-35 138
Msa0548530 AT5G52510.1 27.632 380 247 10 287 649 271 639 6.10e-35 141
Msa0548530 AT1G63100.1 27.336 428 278 12 245 650 213 629 6.55e-34 137
Msa0548530 AT1G63100.2 27.336 428 278 12 245 650 238 654 6.73e-34 138
Msa0548530 AT1G55580.1 26.923 390 214 11 313 649 73 444 5.94e-31 126
Msa0548530 AT4G08250.1 30.194 361 226 14 303 649 128 476 7.60e-31 127
Msa0548530 AT3G49950.1 27.990 393 245 10 284 651 30 409 1.14e-30 125
Msa0548530 AT1G50420.1 30.088 226 143 4 281 497 54 273 1.22e-28 120
Msa0548530 AT1G50420.1 30.667 150 103 1 500 649 330 478 8.88e-17 84.0
Msa0548530 AT3G13840.1 25.822 426 257 20 251 649 116 509 8.51e-20 93.6
Msa0548530 AT2G45160.1 22.025 395 250 13 285 649 272 638 2.66e-12 70.5
Msa0548530 AT3G60630.1 28.502 207 113 6 352 536 327 520 4.24e-11 66.6

Find 129 sgRNAs with CRISPR-Local

Find 314 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGATTCTGTTCCTTCTTC+TGG 0.154876 4_1:+42631885 Msa0548530:CDS
TTCTGCTAGCTGTTTCTTGC+TGG 0.218999 4_1:+42633665 Msa0548530:CDS
AATATTCAAACCCTATTCTT+AGG 0.247868 4_1:+42631943 Msa0548530:CDS
TTCACTCTATCCACCAGTTC+TGG 0.254183 4_1:-42633559 None:intergenic
TCGTCGCAATCACAGATTTC+TGG 0.282921 4_1:+42632479 Msa0548530:CDS
GTATATTAGTGATATTCTTA+TGG 0.285545 4_1:+42631965 Msa0548530:CDS
TCAAAGGATGTCGAATTTCT+TGG 0.296573 4_1:+42632832 Msa0548530:CDS
TCTTATGGATGAAGAAGATT+TGG 0.306482 4_1:+42631980 Msa0548530:CDS
TAAGTACTACGGTAGATTTA+TGG 0.318992 4_1:+42632564 Msa0548530:CDS
ATCTTCCTCAGCCAGGATTT+AGG 0.323032 4_1:+42633005 Msa0548530:CDS
CTCAGAAGATTCACCATTTG+AGG 0.340982 4_1:-42631913 None:intergenic
CCACCAAGGCTTCGTATTAC+AGG 0.346264 4_1:+42632977 Msa0548530:CDS
ACACCATGTATGAAAATGTT+TGG 0.351597 4_1:-42633271 None:intergenic
ACAATTGCCTTGCTCAGAAA+TGG 0.366886 4_1:+42633101 Msa0548530:CDS
TCACTAATATACCTAAGAAT+AGG 0.370302 4_1:-42631954 None:intergenic
TCTGTTAACGGATGCATGTT+TGG 0.374306 4_1:+42631780 Msa0548530:CDS
TCTGGTTCAGGAAGATCTAA+TGG 0.377826 4_1:+42632497 Msa0548530:CDS
CTTACAAGCAGTGGCAGGTT+AGG 0.393985 4_1:+42633500 Msa0548530:CDS
GGATGGGAAGTGGGTTCTGC+AGG 0.394105 4_1:+42633627 Msa0548530:CDS
CAATCACAGATTTCTGGTTC+AGG 0.395582 4_1:+42632485 Msa0548530:CDS
TATCAAACAATGATGAGAAA+TGG 0.404180 4_1:-42633351 None:intergenic
TCTGCTAGCTGTTTCTTGCT+GGG 0.404236 4_1:+42633666 Msa0548530:CDS
TGATGATGTTCTTCTTTGTA+AGG 0.409875 4_1:+42632418 Msa0548530:CDS
ATGATTGAGAAAGGGTTATT+CGG 0.411272 4_1:+42633418 Msa0548530:CDS
GTCTCTTCAACCCTTTCTGC+AGG 0.412650 4_1:-42633028 None:intergenic
ACTTCCATTGGCTCCAGAAC+TGG 0.415867 4_1:+42633546 Msa0548530:CDS
TTCATACATGGTGTAGTGAA+TGG 0.416386 4_1:+42633280 Msa0548530:CDS
AAATTCGACATCCTTTGAAA+TGG 0.417376 4_1:-42632827 None:intergenic
CACTAATATACCTAAGAATA+GGG 0.422212 4_1:-42631953 None:intergenic
ATGATATACTGAAGCAGATT+AGG 0.428145 4_1:+42632642 Msa0548530:CDS
AGTGAAAGAAATGGTGAAGA+AGG 0.428294 4_1:+42633576 Msa0548530:CDS
GTGAAGTACTGCAAGAGGTT+TGG 0.428838 4_1:+42633064 Msa0548530:CDS
TGCCACTGCTTGTAAGTCTC+TGG 0.433473 4_1:-42633493 None:intergenic
AGCCTCCACATTATTGACTT+TGG 0.433913 4_1:+42632893 Msa0548530:CDS
CGTTGTTGATTGCGATTCTC+CGG 0.436900 4_1:+42632142 Msa0548530:CDS
TCATCAACTACAAAATCTTT+CGG 0.438965 4_1:-42633604 None:intergenic
GGTTATCTGATGAATGATAA+TGG 0.445118 4_1:+42632311 Msa0548530:CDS
GATGTTCTGGGATCAGGAAC+TGG 0.454465 4_1:+42632065 Msa0548530:CDS
TCTTCTGGAATAACCTCAAA+TGG 0.456149 4_1:+42631900 Msa0548530:CDS
GCAAGGCAATTGTACTCAAA+TGG 0.459316 4_1:-42633091 None:intergenic
TGATAAAATTCCTCATGATC+CGG 0.461532 4_1:+42632187 Msa0548530:CDS
TCTATATAATTTGATTAAGC+AGG 0.461754 4_1:-42633691 None:intergenic
TTTGAGGTTATTCCAGAAGA+AGG 0.463666 4_1:-42631897 None:intergenic
AAACCTCGCTGATCTCTAAC+CGG 0.468294 4_1:-42632206 None:intergenic
TTAGCTCATTACTTTGCTGA+TGG 0.473385 4_1:+42632701 Msa0548530:CDS
AGGATTTAGGCCTGCAGAAA+GGG 0.473613 4_1:+42633018 Msa0548530:CDS
CTGATGAATGATAATGGTAT+TGG 0.482313 4_1:+42632317 Msa0548530:CDS
TGGGTTCTGCAGGGTTGGAA+AGG 0.485460 4_1:+42633637 Msa0548530:CDS
GGAAGTGGGTTCTGCAGGGT+TGG 0.485717 4_1:+42633632 Msa0548530:CDS
CGCTGAAGTCCATCGCCACT+TGG 0.487409 4_1:-42632677 None:intergenic
AAGACATCGCGATCGAAGAA+AGG 0.489585 4_1:+42632521 Msa0548530:CDS
CAATTGCCTTGCTCAGAAAT+GGG 0.493446 4_1:+42633102 Msa0548530:CDS
ATGCTGTGTTGAGGTTGATC+AGG 0.494919 4_1:+42633239 Msa0548530:CDS
GGGTTCTGCAGGGTTGGAAA+GGG 0.502336 4_1:+42633638 Msa0548530:CDS
CAGGATTTAGGCCTGCAGAA+AGG 0.503621 4_1:+42633017 Msa0548530:CDS
ATCGAAGAAAGGTTCCAGTA+AGG 0.504093 4_1:+42632532 Msa0548530:CDS
AAACAATCTTGCAACATACA+AGG 0.512556 4_1:-42632011 None:intergenic
CTTTCTGCAGGCCTAAATCC+TGG 0.515059 4_1:-42633016 None:intergenic
TATAGGCTGATGATTGAGAA+AGG 0.518213 4_1:+42633409 Msa0548530:CDS
CCTGTAATACGAAGCCTTGG+TGG 0.518595 4_1:-42632977 None:intergenic
GAAGAATGTTTCTGATGAAA+CGG 0.519403 4_1:+42633195 Msa0548530:CDS
GTTTGATATGCTTGAAGCTA+CGG 0.522691 4_1:+42633366 Msa0548530:CDS
CATTCTTCGCCAAGTGGCGA+TGG 0.522850 4_1:+42632668 Msa0548530:CDS
TGAGAAAGGGTTATTCGGAA+GGG 0.524037 4_1:+42633423 Msa0548530:CDS
TTACAGCTTCTCCATTTCAA+AGG 0.527701 4_1:+42632816 Msa0548530:CDS
GGAATTGATCTTCCTCAGCC+AGG 0.529102 4_1:+42632998 Msa0548530:CDS
CAACAGAACTATTCTGAAGC+TGG 0.530899 4_1:+42632856 Msa0548530:CDS
AAGGTTCAAACAACTTCCAT+TGG 0.531648 4_1:+42633534 Msa0548530:CDS
AAGGAGCAGAGAGGGTTGAA+AGG 0.532815 4_1:+42633470 Msa0548530:CDS
TCGAAGAAAGGTTCCAGTAA+GGG 0.534571 4_1:+42632533 Msa0548530:CDS
ATGCATCCGTTAACAGAACC+TGG 0.537307 4_1:-42631774 None:intergenic
GAAGGAACAGAATCACTTGA+AGG 0.539537 4_1:-42631879 None:intergenic
TCCACCAGTTCTGGAGCCAA+TGG 0.540258 4_1:-42633550 None:intergenic
AGCTTGTGAAGGAGCAGAGA+GGG 0.541628 4_1:+42633462 Msa0548530:CDS
CAGCGTCTTTCAGAGAGATC+CGG 0.542215 4_1:+42632950 Msa0548530:CDS
GCAGAAAGGGTTGAAGAGAC+AGG 0.545150 4_1:+42633031 Msa0548530:CDS
GATGGGAAGTGGGTTCTGCA+GGG 0.547301 4_1:+42633628 Msa0548530:CDS
TTGAGAAAGGGTTATTCGGA+AGG 0.547490 4_1:+42633422 Msa0548530:CDS
CAGCTTCAGAATAGTTCTGT+TGG 0.547739 4_1:-42632855 None:intergenic
GATGTTCTTCTTTGTAAGGA+TGG 0.548183 4_1:+42632422 Msa0548530:CDS
AGAGGATTAGGGGAGAAGAG+AGG 0.548379 4_1:+42632290 Msa0548530:CDS
CTTGAGGCGCGATTATCTGC+TGG 0.549108 4_1:+42632725 Msa0548530:CDS
TTACTTTGCTGATGGACTTG+AGG 0.550105 4_1:+42632709 Msa0548530:CDS
GGCCAGAGACTTACAAGCAG+TGG 0.550744 4_1:+42633491 Msa0548530:CDS
TTCAGCTTCCAAACATCACT+CGG 0.553651 4_1:-42632239 None:intergenic
ACGAAGCCTTGGTGGCCCAC+CGG 0.554118 4_1:-42632969 None:intergenic
AGTTGATGAGGATGGGAAGT+GGG 0.558093 4_1:+42633618 Msa0548530:CDS
CGAAGAAAGGTTCCAGTAAG+GGG 0.564272 4_1:+42632534 Msa0548530:CDS
TAGTTGATGAGGATGGGAAG+TGG 0.564567 4_1:+42633617 Msa0548530:CDS
CGGATCTCTCTGAAAGACGC+TGG 0.565013 4_1:-42632949 None:intergenic
TAGTGTCCCATTTCTGAGCA+AGG 0.568684 4_1:-42633108 None:intergenic
TGATAGAGAAGAAGTAACAG+TGG 0.569114 4_1:+42633153 Msa0548530:CDS
TTTGGGTTCTAGAGGATTAG+GGG 0.570677 4_1:+42632280 Msa0548530:CDS
AGAGACTTACAAGCAGTGGC+AGG 0.571929 4_1:+42633495 Msa0548530:CDS
CTGCAGCAGACGACGACTGA+AGG 0.573929 4_1:-42632766 None:intergenic
ATTCCTGTAATACGAAGCCT+TGG 0.575936 4_1:-42632980 None:intergenic
CGTCTTTCAGAGAGATCCGG+TGG 0.576548 4_1:+42632953 Msa0548530:CDS
AACCTCAACACAGCATCTCT+AGG 0.577064 4_1:-42633232 None:intergenic
ATAGGCTGATGATTGAGAAA+GGG 0.583749 4_1:+42633410 Msa0548530:CDS
TCTCAATGTGCACAAGCTGT+TGG 0.587807 4_1:+42632596 Msa0548530:CDS
AGGTTAGGAACAAGAGAGCA+AGG 0.590161 4_1:+42633515 Msa0548530:CDS
TCCATTGGCTCCAGAACTGG+TGG 0.592807 4_1:+42633549 Msa0548530:CDS
GACTTGTGAAGTACTGCAAG+AGG 0.596425 4_1:+42633059 Msa0548530:CDS
AGAGATCCGGTGGGCCACCA+AGG 0.598231 4_1:+42632963 Msa0548530:CDS
CTGTTGGTAGCTATGATCAA+AGG 0.603395 4_1:+42632612 Msa0548530:CDS
CGGAGAATCGCAATCAACAA+CGG 0.604253 4_1:-42632141 None:intergenic
CGCGTGAAGATGATCAATAT+AGG 0.605875 4_1:+42633392 Msa0548530:CDS
GCAGGCCTAAATCCTGGCTG+AGG 0.614834 4_1:-42633010 None:intergenic
CTGTGTTGAGGTTGATCAGG+AGG 0.616107 4_1:+42633242 Msa0548530:CDS
GGTGGATAGAGTGAAAGAAA+TGG 0.616135 4_1:+42633567 Msa0548530:CDS
AAATACCAAAGTCAATAATG+TGG 0.616394 4_1:-42632898 None:intergenic
GTCCTAGAGATGCTGTGTTG+AGG 0.620188 4_1:+42633230 Msa0548530:CDS
GTTTGGAAGCTGAATGAAGA+GGG 0.621160 4_1:+42632248 Msa0548530:CDS
ATGGGACACTATAAGACTTG+AGG 0.623248 4_1:+42633120 Msa0548530:CDS
TACCAAAGTCAATAATGTGG+AGG 0.623436 4_1:-42632895 None:intergenic
TGATCTCTAACCGGATCATG+AGG 0.623977 4_1:-42632197 None:intergenic
GAAAGACGCTGGATGAGACA+AGG 0.629384 4_1:-42632938 None:intergenic
AGGCAGCATTCTTCGCCAAG+TGG 0.643469 4_1:+42632662 Msa0548530:CDS
AGAAGATCTGAGAAGTAACA+AGG 0.644638 4_1:+42632352 Msa0548530:CDS
AAAGGGTTGAAGAGACAGGT+CGG 0.646693 4_1:+42633035 Msa0548530:CDS
TGTTTCTGATGAAACGGTGA+CGG 0.654600 4_1:+42633201 Msa0548530:CDS
GTCTTTCAGAGAGATCCGGT+GGG 0.679118 4_1:+42632954 Msa0548530:CDS
CTATATTGATCATCTTCACG+CGG 0.683616 4_1:-42633391 None:intergenic
TCCGGAAAATAATGCATACG+AGG 0.702672 4_1:+42632160 Msa0548530:CDS
GTAAATGTGATAGCTTGTGA+AGG 0.707067 4_1:+42633451 Msa0548530:CDS
TAGCTTGTGAAGGAGCAGAG+AGG 0.713278 4_1:+42633461 Msa0548530:CDS
TGTTTGGAAGCTGAATGAAG+AGG 0.744567 4_1:+42632247 Msa0548530:CDS
GACTGAAGGAGTTTATACAT+CGG 0.748275 4_1:-42632752 None:intergenic
GATCCGGTTAGAGATCAGCG+AGG 0.803140 4_1:+42632203 Msa0548530:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTATATTAGTGATATTCTTA+TGG + chr4_1:42631965-42631984 Msa0548530:CDS 20.0%
!! GTATATTAGTGATATTCTTA+TGG + chr4_1:42631965-42631984 Msa0548530:CDS 20.0%
! AATATTCAAACCCTATTCTT+AGG + chr4_1:42631943-42631962 Msa0548530:CDS 25.0%
! CACTAATATACCTAAGAATA+GGG - chr4_1:42631956-42631975 None:intergenic 25.0%
! TCACTAATATACCTAAGAAT+AGG - chr4_1:42631957-42631976 None:intergenic 25.0%
!! AAAATCGTTTTATGATGTTC+TGG + chr4_1:42632052-42632071 Msa0548530:CDS 25.0%
!! AAATCGTTTTATGATGTTCT+GGG + chr4_1:42632053-42632072 Msa0548530:CDS 25.0%
! GAATTATCACAAAAAATCGA+CGG - chr4_1:42632455-42632474 None:intergenic 25.0%
! AAATACCAAAGTCAATAATG+TGG - chr4_1:42632901-42632920 None:intergenic 25.0%
!!! ATTGACTTTGGTATTTTCTA+TGG + chr4_1:42632905-42632924 Msa0548530:CDS 25.0%
!! AATCCAAACATTTTCATACA+TGG + chr4_1:42633268-42633287 Msa0548530:CDS 25.0%
! TATCAAACAATGATGAGAAA+TGG - chr4_1:42633354-42633373 None:intergenic 25.0%
! TCATCAACTACAAAATCTTT+CGG - chr4_1:42633607-42633626 None:intergenic 25.0%
! AATATTCAAACCCTATTCTT+AGG + chr4_1:42631943-42631962 Msa0548530:CDS 25.0%
! CACTAATATACCTAAGAATA+GGG - chr4_1:42631956-42631975 None:intergenic 25.0%
! TCACTAATATACCTAAGAAT+AGG - chr4_1:42631957-42631976 None:intergenic 25.0%
!! AAAATCGTTTTATGATGTTC+TGG + chr4_1:42632052-42632071 Msa0548530:CDS 25.0%
!! AAATCGTTTTATGATGTTCT+GGG + chr4_1:42632053-42632072 Msa0548530:CDS 25.0%
! GAATTATCACAAAAAATCGA+CGG - chr4_1:42632455-42632474 None:intergenic 25.0%
! AAATACCAAAGTCAATAATG+TGG - chr4_1:42632901-42632920 None:intergenic 25.0%
!!! ATTGACTTTGGTATTTTCTA+TGG + chr4_1:42632905-42632924 Msa0548530:CDS 25.0%
!! AATCCAAACATTTTCATACA+TGG + chr4_1:42633268-42633287 Msa0548530:CDS 25.0%
! TATCAAACAATGATGAGAAA+TGG - chr4_1:42633354-42633373 None:intergenic 25.0%
! TCATCAACTACAAAATCTTT+CGG - chr4_1:42633607-42633626 None:intergenic 25.0%
! AATTTTCCAGGTTCTGTTAA+CGG + chr4_1:42631768-42631787 Msa0548530:CDS 30.0%
! TTGGTTTGAAAAAACAGACA+CGG - chr4_1:42631814-42631833 None:intergenic 30.0%
TCTTATGGATGAAGAAGATT+TGG + chr4_1:42631980-42631999 Msa0548530:CDS 30.0%
AAACAATCTTGCAACATACA+AGG - chr4_1:42632014-42632033 None:intergenic 30.0%
TGATAAAATTCCTCATGATC+CGG + chr4_1:42632187-42632206 Msa0548530:CDS 30.0%
GGTTATCTGATGAATGATAA+TGG + chr4_1:42632311-42632330 Msa0548530:CDS 30.0%
!! CTGATGAATGATAATGGTAT+TGG + chr4_1:42632317-42632336 Msa0548530:CDS 30.0%
! TGATGATGTTCTTCTTTGTA+AGG + chr4_1:42632418-42632437 Msa0548530:CDS 30.0%
TAAGTACTACGGTAGATTTA+TGG + chr4_1:42632564-42632583 Msa0548530:CDS 30.0%
ATGATATACTGAAGCAGATT+AGG + chr4_1:42632642-42632661 Msa0548530:CDS 30.0%
! AAATTCGACATCCTTTGAAA+TGG - chr4_1:42632830-42632849 None:intergenic 30.0%
! GAAGAATGTTTCTGATGAAA+CGG + chr4_1:42633195-42633214 Msa0548530:CDS 30.0%
ACACCATGTATGAAAATGTT+TGG - chr4_1:42633274-42633293 None:intergenic 30.0%
CTGAACCTAGTAAGAAAAAA+TGG - chr4_1:42633319-42633338 None:intergenic 30.0%
ATGATTGAGAAAGGGTTATT+CGG + chr4_1:42633418-42633437 Msa0548530:CDS 30.0%
!! GAAAGATTTTGTAGTTGATG+AGG + chr4_1:42633606-42633625 Msa0548530:CDS 30.0%
!!! ATTTTGTAGTTGATGAGGAT+GGG + chr4_1:42633611-42633630 Msa0548530:CDS 30.0%
! AATTTTCCAGGTTCTGTTAA+CGG + chr4_1:42631768-42631787 Msa0548530:CDS 30.0%
! TTGGTTTGAAAAAACAGACA+CGG - chr4_1:42631814-42631833 None:intergenic 30.0%
TCTTATGGATGAAGAAGATT+TGG + chr4_1:42631980-42631999 Msa0548530:CDS 30.0%
AAACAATCTTGCAACATACA+AGG - chr4_1:42632014-42632033 None:intergenic 30.0%
TGATAAAATTCCTCATGATC+CGG + chr4_1:42632187-42632206 Msa0548530:CDS 30.0%
GGTTATCTGATGAATGATAA+TGG + chr4_1:42632311-42632330 Msa0548530:CDS 30.0%
!! CTGATGAATGATAATGGTAT+TGG + chr4_1:42632317-42632336 Msa0548530:CDS 30.0%
! TGATGATGTTCTTCTTTGTA+AGG + chr4_1:42632418-42632437 Msa0548530:CDS 30.0%
TAAGTACTACGGTAGATTTA+TGG + chr4_1:42632564-42632583 Msa0548530:CDS 30.0%
ATGATATACTGAAGCAGATT+AGG + chr4_1:42632642-42632661 Msa0548530:CDS 30.0%
! AAATTCGACATCCTTTGAAA+TGG - chr4_1:42632830-42632849 None:intergenic 30.0%
! GAAGAATGTTTCTGATGAAA+CGG + chr4_1:42633195-42633214 Msa0548530:CDS 30.0%
ACACCATGTATGAAAATGTT+TGG - chr4_1:42633274-42633293 None:intergenic 30.0%
CTGAACCTAGTAAGAAAAAA+TGG - chr4_1:42633319-42633338 None:intergenic 30.0%
ATGATTGAGAAAGGGTTATT+CGG + chr4_1:42633418-42633437 Msa0548530:CDS 30.0%
!! GAAAGATTTTGTAGTTGATG+AGG + chr4_1:42633606-42633625 Msa0548530:CDS 30.0%
!!! ATTTTGTAGTTGATGAGGAT+GGG + chr4_1:42633611-42633630 Msa0548530:CDS 30.0%
! ACAGTCGTAGAAAATTTTCC+AGG + chr4_1:42631756-42631775 Msa0548530:CDS 35.0%
TGGTTTGAAAAAACAGACAC+GGG - chr4_1:42631813-42631832 None:intergenic 35.0%
! CGAATTTAAACGCGTTTTCT+GGG - chr4_1:42631839-42631858 None:intergenic 35.0%
! TCGAATTTAAACGCGTTTTC+TGG - chr4_1:42631840-42631859 None:intergenic 35.0%
! TTTGAGGTTATTCCAGAAGA+AGG - chr4_1:42631900-42631919 None:intergenic 35.0%
!! TCTTCTGGAATAACCTCAAA+TGG + chr4_1:42631900-42631919 Msa0548530:CDS 35.0%
!!! TTTTATGATGTTCTGGGATC+AGG + chr4_1:42632059-42632078 Msa0548530:CDS 35.0%
! ACCTCGTATGCATTATTTTC+CGG - chr4_1:42632164-42632183 None:intergenic 35.0%
!!! TGAAGAGGGTAGTTTTGTTT+TGG + chr4_1:42632262-42632281 Msa0548530:CDS 35.0%
!!! GAAGAGGGTAGTTTTGTTTT+GGG + chr4_1:42632263-42632282 Msa0548530:CDS 35.0%
!!! AGTTTTGTTTTGGGTTCTAG+AGG + chr4_1:42632272-42632291 Msa0548530:CDS 35.0%
!!! TTTTGGGTTCTAGAGGATTA+GGG + chr4_1:42632279-42632298 Msa0548530:CDS 35.0%
AGAAGATCTGAGAAGTAACA+AGG + chr4_1:42632352-42632371 Msa0548530:CDS 35.0%
GATGTTCTTCTTTGTAAGGA+TGG + chr4_1:42632422-42632441 Msa0548530:CDS 35.0%
!!! ACTTAGTTTTTTCCCCTTAC+TGG - chr4_1:42632549-42632568 None:intergenic 35.0%
GGGGAAAAAACTAAGTACTA+CGG + chr4_1:42632553-42632572 Msa0548530:CDS 35.0%
TTAGCTCATTACTTTGCTGA+TGG + chr4_1:42632701-42632720 Msa0548530:CDS 35.0%
GACTGAAGGAGTTTATACAT+CGG - chr4_1:42632755-42632774 None:intergenic 35.0%
TTACAGCTTCTCCATTTCAA+AGG + chr4_1:42632816-42632835 Msa0548530:CDS 35.0%
TCAAAGGATGTCGAATTTCT+TGG + chr4_1:42632832-42632851 Msa0548530:CDS 35.0%
TACCAAAGTCAATAATGTGG+AGG - chr4_1:42632898-42632917 None:intergenic 35.0%
! GTATTTTCTATGGCTTTCAG+TGG + chr4_1:42632915-42632934 Msa0548530:CDS 35.0%
TGATAGAGAAGAAGTAACAG+TGG + chr4_1:42633153-42633172 Msa0548530:CDS 35.0%
TTCATACATGGTGTAGTGAA+TGG + chr4_1:42633280-42633299 Msa0548530:CDS 35.0%
!!! ACGCACCATTTTTTCTTACT+AGG + chr4_1:42633311-42633330 Msa0548530:CDS 35.0%
GTTTGATATGCTTGAAGCTA+CGG + chr4_1:42633366-42633385 Msa0548530:CDS 35.0%
CTATATTGATCATCTTCACG+CGG - chr4_1:42633394-42633413 None:intergenic 35.0%
TATAGGCTGATGATTGAGAA+AGG + chr4_1:42633409-42633428 Msa0548530:CDS 35.0%
ATAGGCTGATGATTGAGAAA+GGG + chr4_1:42633410-42633429 Msa0548530:CDS 35.0%
! GTAAATGTGATAGCTTGTGA+AGG + chr4_1:42633451-42633470 Msa0548530:CDS 35.0%
AAGGTTCAAACAACTTCCAT+TGG + chr4_1:42633534-42633553 Msa0548530:CDS 35.0%
AGTGAAAGAAATGGTGAAGA+AGG + chr4_1:42633576-42633595 Msa0548530:CDS 35.0%
!!! GATTTTGTAGTTGATGAGGA+TGG + chr4_1:42633610-42633629 Msa0548530:CDS 35.0%
! ACAGTCGTAGAAAATTTTCC+AGG + chr4_1:42631756-42631775 Msa0548530:CDS 35.0%
TGGTTTGAAAAAACAGACAC+GGG - chr4_1:42631813-42631832 None:intergenic 35.0%
! CGAATTTAAACGCGTTTTCT+GGG - chr4_1:42631839-42631858 None:intergenic 35.0%
! TCGAATTTAAACGCGTTTTC+TGG - chr4_1:42631840-42631859 None:intergenic 35.0%
! TTTGAGGTTATTCCAGAAGA+AGG - chr4_1:42631900-42631919 None:intergenic 35.0%
!! TCTTCTGGAATAACCTCAAA+TGG + chr4_1:42631900-42631919 Msa0548530:CDS 35.0%
!!! TTTTATGATGTTCTGGGATC+AGG + chr4_1:42632059-42632078 Msa0548530:CDS 35.0%
! ACCTCGTATGCATTATTTTC+CGG - chr4_1:42632164-42632183 None:intergenic 35.0%
!!! TGAAGAGGGTAGTTTTGTTT+TGG + chr4_1:42632262-42632281 Msa0548530:CDS 35.0%
!!! GAAGAGGGTAGTTTTGTTTT+GGG + chr4_1:42632263-42632282 Msa0548530:CDS 35.0%
!!! AGTTTTGTTTTGGGTTCTAG+AGG + chr4_1:42632272-42632291 Msa0548530:CDS 35.0%
!!! TTTTGGGTTCTAGAGGATTA+GGG + chr4_1:42632279-42632298 Msa0548530:CDS 35.0%
AGAAGATCTGAGAAGTAACA+AGG + chr4_1:42632352-42632371 Msa0548530:CDS 35.0%
GATGTTCTTCTTTGTAAGGA+TGG + chr4_1:42632422-42632441 Msa0548530:CDS 35.0%
!!! ACTTAGTTTTTTCCCCTTAC+TGG - chr4_1:42632549-42632568 None:intergenic 35.0%
GGGGAAAAAACTAAGTACTA+CGG + chr4_1:42632553-42632572 Msa0548530:CDS 35.0%
TTAGCTCATTACTTTGCTGA+TGG + chr4_1:42632701-42632720 Msa0548530:CDS 35.0%
GACTGAAGGAGTTTATACAT+CGG - chr4_1:42632755-42632774 None:intergenic 35.0%
TTACAGCTTCTCCATTTCAA+AGG + chr4_1:42632816-42632835 Msa0548530:CDS 35.0%
TCAAAGGATGTCGAATTTCT+TGG + chr4_1:42632832-42632851 Msa0548530:CDS 35.0%
TACCAAAGTCAATAATGTGG+AGG - chr4_1:42632898-42632917 None:intergenic 35.0%
! GTATTTTCTATGGCTTTCAG+TGG + chr4_1:42632915-42632934 Msa0548530:CDS 35.0%
TGATAGAGAAGAAGTAACAG+TGG + chr4_1:42633153-42633172 Msa0548530:CDS 35.0%
TTCATACATGGTGTAGTGAA+TGG + chr4_1:42633280-42633299 Msa0548530:CDS 35.0%
!!! ACGCACCATTTTTTCTTACT+AGG + chr4_1:42633311-42633330 Msa0548530:CDS 35.0%
GTTTGATATGCTTGAAGCTA+CGG + chr4_1:42633366-42633385 Msa0548530:CDS 35.0%
CTATATTGATCATCTTCACG+CGG - chr4_1:42633394-42633413 None:intergenic 35.0%
TATAGGCTGATGATTGAGAA+AGG + chr4_1:42633409-42633428 Msa0548530:CDS 35.0%
ATAGGCTGATGATTGAGAAA+GGG + chr4_1:42633410-42633429 Msa0548530:CDS 35.0%
! GTAAATGTGATAGCTTGTGA+AGG + chr4_1:42633451-42633470 Msa0548530:CDS 35.0%
AAGGTTCAAACAACTTCCAT+TGG + chr4_1:42633534-42633553 Msa0548530:CDS 35.0%
AGTGAAAGAAATGGTGAAGA+AGG + chr4_1:42633576-42633595 Msa0548530:CDS 35.0%
!!! GATTTTGTAGTTGATGAGGA+TGG + chr4_1:42633610-42633629 Msa0548530:CDS 35.0%
! TCTGTTAACGGATGCATGTT+TGG + chr4_1:42631780-42631799 Msa0548530:CDS 40.0%
! TAAACGCGTTTTCTGGGTTT+TGG - chr4_1:42631833-42631852 None:intergenic 40.0%
GAAGGAACAGAATCACTTGA+AGG - chr4_1:42631882-42631901 None:intergenic 40.0%
AGTGATTCTGTTCCTTCTTC+TGG + chr4_1:42631885-42631904 Msa0548530:CDS 40.0%
CTCAGAAGATTCACCATTTG+AGG - chr4_1:42631916-42631935 None:intergenic 40.0%
TCCGGAAAATAATGCATACG+AGG + chr4_1:42632160-42632179 Msa0548530:CDS 40.0%
!!! AGAGATCAGCGAGGTTTTTT+CGG + chr4_1:42632212-42632231 Msa0548530:CDS 40.0%
TTCAGCTTCCAAACATCACT+CGG - chr4_1:42632242-42632261 None:intergenic 40.0%
TGTTTGGAAGCTGAATGAAG+AGG + chr4_1:42632247-42632266 Msa0548530:CDS 40.0%
GTTTGGAAGCTGAATGAAGA+GGG + chr4_1:42632248-42632267 Msa0548530:CDS 40.0%
!!! GTTTTGGGTTCTAGAGGATT+AGG + chr4_1:42632278-42632297 Msa0548530:CDS 40.0%
!! TTTGGGTTCTAGAGGATTAG+GGG + chr4_1:42632280-42632299 Msa0548530:CDS 40.0%
!! CAATCACAGATTTCTGGTTC+AGG + chr4_1:42632485-42632504 Msa0548530:CDS 40.0%
! TCTGGTTCAGGAAGATCTAA+TGG + chr4_1:42632497-42632516 Msa0548530:CDS 40.0%
ATCGAAGAAAGGTTCCAGTA+AGG + chr4_1:42632532-42632551 Msa0548530:CDS 40.0%
TCGAAGAAAGGTTCCAGTAA+GGG + chr4_1:42632533-42632552 Msa0548530:CDS 40.0%
!! CTGTTGGTAGCTATGATCAA+AGG + chr4_1:42632612-42632631 Msa0548530:CDS 40.0%
!! TTACTTTGCTGATGGACTTG+AGG + chr4_1:42632709-42632728 Msa0548530:CDS 40.0%
! CAGCTTCAGAATAGTTCTGT+TGG - chr4_1:42632858-42632877 None:intergenic 40.0%
!! CAACAGAACTATTCTGAAGC+TGG + chr4_1:42632856-42632875 Msa0548530:CDS 40.0%
!! AGCCTCCACATTATTGACTT+TGG + chr4_1:42632893-42632912 Msa0548530:CDS 40.0%
ATTCCTGTAATACGAAGCCT+TGG - chr4_1:42632983-42633002 None:intergenic 40.0%
GCAAGGCAATTGTACTCAAA+TGG - chr4_1:42633094-42633113 None:intergenic 40.0%
ACAATTGCCTTGCTCAGAAA+TGG + chr4_1:42633101-42633120 Msa0548530:CDS 40.0%
CAATTGCCTTGCTCAGAAAT+GGG + chr4_1:42633102-42633121 Msa0548530:CDS 40.0%
ATGGGACACTATAAGACTTG+AGG + chr4_1:42633120-42633139 Msa0548530:CDS 40.0%
! TGTTTCTGATGAAACGGTGA+CGG + chr4_1:42633201-42633220 Msa0548530:CDS 40.0%
CGCGTGAAGATGATCAATAT+AGG + chr4_1:42633392-42633411 Msa0548530:CDS 40.0%
TTGAGAAAGGGTTATTCGGA+AGG + chr4_1:42633422-42633441 Msa0548530:CDS 40.0%
TGAGAAAGGGTTATTCGGAA+GGG + chr4_1:42633423-42633442 Msa0548530:CDS 40.0%
GGTGGATAGAGTGAAAGAAA+TGG + chr4_1:42633567-42633586 Msa0548530:CDS 40.0%
! TCTGTTAACGGATGCATGTT+TGG + chr4_1:42631780-42631799 Msa0548530:CDS 40.0%
! TAAACGCGTTTTCTGGGTTT+TGG - chr4_1:42631833-42631852 None:intergenic 40.0%
GAAGGAACAGAATCACTTGA+AGG - chr4_1:42631882-42631901 None:intergenic 40.0%
AGTGATTCTGTTCCTTCTTC+TGG + chr4_1:42631885-42631904 Msa0548530:CDS 40.0%
CTCAGAAGATTCACCATTTG+AGG - chr4_1:42631916-42631935 None:intergenic 40.0%
TCCGGAAAATAATGCATACG+AGG + chr4_1:42632160-42632179 Msa0548530:CDS 40.0%
!!! AGAGATCAGCGAGGTTTTTT+CGG + chr4_1:42632212-42632231 Msa0548530:CDS 40.0%
TTCAGCTTCCAAACATCACT+CGG - chr4_1:42632242-42632261 None:intergenic 40.0%
TGTTTGGAAGCTGAATGAAG+AGG + chr4_1:42632247-42632266 Msa0548530:CDS 40.0%
GTTTGGAAGCTGAATGAAGA+GGG + chr4_1:42632248-42632267 Msa0548530:CDS 40.0%
!!! GTTTTGGGTTCTAGAGGATT+AGG + chr4_1:42632278-42632297 Msa0548530:CDS 40.0%
!! TTTGGGTTCTAGAGGATTAG+GGG + chr4_1:42632280-42632299 Msa0548530:CDS 40.0%
!! CAATCACAGATTTCTGGTTC+AGG + chr4_1:42632485-42632504 Msa0548530:CDS 40.0%
! TCTGGTTCAGGAAGATCTAA+TGG + chr4_1:42632497-42632516 Msa0548530:CDS 40.0%
ATCGAAGAAAGGTTCCAGTA+AGG + chr4_1:42632532-42632551 Msa0548530:CDS 40.0%
TCGAAGAAAGGTTCCAGTAA+GGG + chr4_1:42632533-42632552 Msa0548530:CDS 40.0%
!! CTGTTGGTAGCTATGATCAA+AGG + chr4_1:42632612-42632631 Msa0548530:CDS 40.0%
!! TTACTTTGCTGATGGACTTG+AGG + chr4_1:42632709-42632728 Msa0548530:CDS 40.0%
! CAGCTTCAGAATAGTTCTGT+TGG - chr4_1:42632858-42632877 None:intergenic 40.0%
!! CAACAGAACTATTCTGAAGC+TGG + chr4_1:42632856-42632875 Msa0548530:CDS 40.0%
!! AGCCTCCACATTATTGACTT+TGG + chr4_1:42632893-42632912 Msa0548530:CDS 40.0%
ATTCCTGTAATACGAAGCCT+TGG - chr4_1:42632983-42633002 None:intergenic 40.0%
GCAAGGCAATTGTACTCAAA+TGG - chr4_1:42633094-42633113 None:intergenic 40.0%
ACAATTGCCTTGCTCAGAAA+TGG + chr4_1:42633101-42633120 Msa0548530:CDS 40.0%
CAATTGCCTTGCTCAGAAAT+GGG + chr4_1:42633102-42633121 Msa0548530:CDS 40.0%
ATGGGACACTATAAGACTTG+AGG + chr4_1:42633120-42633139 Msa0548530:CDS 40.0%
! TGTTTCTGATGAAACGGTGA+CGG + chr4_1:42633201-42633220 Msa0548530:CDS 40.0%
CGCGTGAAGATGATCAATAT+AGG + chr4_1:42633392-42633411 Msa0548530:CDS 40.0%
TTGAGAAAGGGTTATTCGGA+AGG + chr4_1:42633422-42633441 Msa0548530:CDS 40.0%
TGAGAAAGGGTTATTCGGAA+GGG + chr4_1:42633423-42633442 Msa0548530:CDS 40.0%
GGTGGATAGAGTGAAAGAAA+TGG + chr4_1:42633567-42633586 Msa0548530:CDS 40.0%
ATGCATCCGTTAACAGAACC+TGG - chr4_1:42631777-42631796 None:intergenic 45.0%
CGGAGAATCGCAATCAACAA+CGG - chr4_1:42632144-42632163 None:intergenic 45.0%
! CGTTGTTGATTGCGATTCTC+CGG + chr4_1:42632142-42632161 Msa0548530:CDS 45.0%
TGATCTCTAACCGGATCATG+AGG - chr4_1:42632200-42632219 None:intergenic 45.0%
AAACCTCGCTGATCTCTAAC+CGG - chr4_1:42632209-42632228 None:intergenic 45.0%
! TCGGTTTTCCGAGTGATGTT+TGG + chr4_1:42632231-42632250 Msa0548530:CDS 45.0%
TCGTCGCAATCACAGATTTC+TGG + chr4_1:42632479-42632498 Msa0548530:CDS 45.0%
AAGACATCGCGATCGAAGAA+AGG + chr4_1:42632521-42632540 Msa0548530:CDS 45.0%
CGAAGAAAGGTTCCAGTAAG+GGG + chr4_1:42632534-42632553 Msa0548530:CDS 45.0%
TCTCAATGTGCACAAGCTGT+TGG + chr4_1:42632596-42632615 Msa0548530:CDS 45.0%
ATCTTCCTCAGCCAGGATTT+AGG + chr4_1:42633005-42633024 Msa0548530:CDS 45.0%
AGGATTTAGGCCTGCAGAAA+GGG + chr4_1:42633018-42633037 Msa0548530:CDS 45.0%
AAAGGGTTGAAGAGACAGGT+CGG + chr4_1:42633035-42633054 Msa0548530:CDS 45.0%
!! GACTTGTGAAGTACTGCAAG+AGG + chr4_1:42633059-42633078 Msa0548530:CDS 45.0%
!! GTGAAGTACTGCAAGAGGTT+TGG + chr4_1:42633064-42633083 Msa0548530:CDS 45.0%
TAGTGTCCCATTTCTGAGCA+AGG - chr4_1:42633111-42633130 None:intergenic 45.0%
AACCTCAACACAGCATCTCT+AGG - chr4_1:42633235-42633254 None:intergenic 45.0%
! ATGCTGTGTTGAGGTTGATC+AGG + chr4_1:42633239-42633258 Msa0548530:CDS 45.0%
AGGTTAGGAACAAGAGAGCA+AGG + chr4_1:42633515-42633534 Msa0548530:CDS 45.0%
TTCACTCTATCCACCAGTTC+TGG - chr4_1:42633562-42633581 None:intergenic 45.0%
!! TAGTTGATGAGGATGGGAAG+TGG + chr4_1:42633617-42633636 Msa0548530:CDS 45.0%
!! AGTTGATGAGGATGGGAAGT+GGG + chr4_1:42633618-42633637 Msa0548530:CDS 45.0%
!! TTCTGCTAGCTGTTTCTTGC+TGG + chr4_1:42633665-42633684 Msa0548530:CDS 45.0%
!! TCTGCTAGCTGTTTCTTGCT+GGG + chr4_1:42633666-42633685 Msa0548530:CDS 45.0%
ATGCATCCGTTAACAGAACC+TGG - chr4_1:42631777-42631796 None:intergenic 45.0%
CGGAGAATCGCAATCAACAA+CGG - chr4_1:42632144-42632163 None:intergenic 45.0%
! CGTTGTTGATTGCGATTCTC+CGG + chr4_1:42632142-42632161 Msa0548530:CDS 45.0%
TGATCTCTAACCGGATCATG+AGG - chr4_1:42632200-42632219 None:intergenic 45.0%
AAACCTCGCTGATCTCTAAC+CGG - chr4_1:42632209-42632228 None:intergenic 45.0%
! TCGGTTTTCCGAGTGATGTT+TGG + chr4_1:42632231-42632250 Msa0548530:CDS 45.0%
TCGTCGCAATCACAGATTTC+TGG + chr4_1:42632479-42632498 Msa0548530:CDS 45.0%
AAGACATCGCGATCGAAGAA+AGG + chr4_1:42632521-42632540 Msa0548530:CDS 45.0%
CGAAGAAAGGTTCCAGTAAG+GGG + chr4_1:42632534-42632553 Msa0548530:CDS 45.0%
TCTCAATGTGCACAAGCTGT+TGG + chr4_1:42632596-42632615 Msa0548530:CDS 45.0%
ATCTTCCTCAGCCAGGATTT+AGG + chr4_1:42633005-42633024 Msa0548530:CDS 45.0%
AGGATTTAGGCCTGCAGAAA+GGG + chr4_1:42633018-42633037 Msa0548530:CDS 45.0%
AAAGGGTTGAAGAGACAGGT+CGG + chr4_1:42633035-42633054 Msa0548530:CDS 45.0%
!! GACTTGTGAAGTACTGCAAG+AGG + chr4_1:42633059-42633078 Msa0548530:CDS 45.0%
!! GTGAAGTACTGCAAGAGGTT+TGG + chr4_1:42633064-42633083 Msa0548530:CDS 45.0%
TAGTGTCCCATTTCTGAGCA+AGG - chr4_1:42633111-42633130 None:intergenic 45.0%
AACCTCAACACAGCATCTCT+AGG - chr4_1:42633235-42633254 None:intergenic 45.0%
! ATGCTGTGTTGAGGTTGATC+AGG + chr4_1:42633239-42633258 Msa0548530:CDS 45.0%
AGGTTAGGAACAAGAGAGCA+AGG + chr4_1:42633515-42633534 Msa0548530:CDS 45.0%
TTCACTCTATCCACCAGTTC+TGG - chr4_1:42633562-42633581 None:intergenic 45.0%
!! TAGTTGATGAGGATGGGAAG+TGG + chr4_1:42633617-42633636 Msa0548530:CDS 45.0%
!! AGTTGATGAGGATGGGAAGT+GGG + chr4_1:42633618-42633637 Msa0548530:CDS 45.0%
!! TTCTGCTAGCTGTTTCTTGC+TGG + chr4_1:42633665-42633684 Msa0548530:CDS 45.0%
!! TCTGCTAGCTGTTTCTTGCT+GGG + chr4_1:42633666-42633685 Msa0548530:CDS 45.0%
!! GATGTTCTGGGATCAGGAAC+TGG + chr4_1:42632065-42632084 Msa0548530:CDS 50.0%
AGAGGATTAGGGGAGAAGAG+AGG + chr4_1:42632290-42632309 Msa0548530:CDS 50.0%
GAAAGACGCTGGATGAGACA+AGG - chr4_1:42632941-42632960 None:intergenic 50.0%
CAGCGTCTTTCAGAGAGATC+CGG + chr4_1:42632950-42632969 Msa0548530:CDS 50.0%
GTCTTTCAGAGAGATCCGGT+GGG + chr4_1:42632954-42632973 Msa0548530:CDS 50.0%
CCTGTAATACGAAGCCTTGG+TGG - chr4_1:42632980-42632999 None:intergenic 50.0%
CCACCAAGGCTTCGTATTAC+AGG + chr4_1:42632977-42632996 Msa0548530:CDS 50.0%
GGAATTGATCTTCCTCAGCC+AGG + chr4_1:42632998-42633017 Msa0548530:CDS 50.0%
CTTTCTGCAGGCCTAAATCC+TGG - chr4_1:42633019-42633038 None:intergenic 50.0%
CAGGATTTAGGCCTGCAGAA+AGG + chr4_1:42633017-42633036 Msa0548530:CDS 50.0%
GTCTCTTCAACCCTTTCTGC+AGG - chr4_1:42633031-42633050 None:intergenic 50.0%
GCAGAAAGGGTTGAAGAGAC+AGG + chr4_1:42633031-42633050 Msa0548530:CDS 50.0%
GTCCTAGAGATGCTGTGTTG+AGG + chr4_1:42633230-42633249 Msa0548530:CDS 50.0%
! CTGTGTTGAGGTTGATCAGG+AGG + chr4_1:42633242-42633261 Msa0548530:CDS 50.0%
! TAGCTTGTGAAGGAGCAGAG+AGG + chr4_1:42633461-42633480 Msa0548530:CDS 50.0%
AGCTTGTGAAGGAGCAGAGA+GGG + chr4_1:42633462-42633481 Msa0548530:CDS 50.0%
AAGGAGCAGAGAGGGTTGAA+AGG + chr4_1:42633470-42633489 Msa0548530:CDS 50.0%
TGCCACTGCTTGTAAGTCTC+TGG - chr4_1:42633496-42633515 None:intergenic 50.0%
AGAGACTTACAAGCAGTGGC+AGG + chr4_1:42633495-42633514 Msa0548530:CDS 50.0%
CTTACAAGCAGTGGCAGGTT+AGG + chr4_1:42633500-42633519 Msa0548530:CDS 50.0%
!! ACTTCCATTGGCTCCAGAAC+TGG + chr4_1:42633546-42633565 Msa0548530:CDS 50.0%
!! GATGTTCTGGGATCAGGAAC+TGG + chr4_1:42632065-42632084 Msa0548530:CDS 50.0%
AGAGGATTAGGGGAGAAGAG+AGG + chr4_1:42632290-42632309 Msa0548530:CDS 50.0%
GAAAGACGCTGGATGAGACA+AGG - chr4_1:42632941-42632960 None:intergenic 50.0%
CAGCGTCTTTCAGAGAGATC+CGG + chr4_1:42632950-42632969 Msa0548530:CDS 50.0%
GTCTTTCAGAGAGATCCGGT+GGG + chr4_1:42632954-42632973 Msa0548530:CDS 50.0%
CCTGTAATACGAAGCCTTGG+TGG - chr4_1:42632980-42632999 None:intergenic 50.0%
CCACCAAGGCTTCGTATTAC+AGG + chr4_1:42632977-42632996 Msa0548530:CDS 50.0%
GGAATTGATCTTCCTCAGCC+AGG + chr4_1:42632998-42633017 Msa0548530:CDS 50.0%
CTTTCTGCAGGCCTAAATCC+TGG - chr4_1:42633019-42633038 None:intergenic 50.0%
CAGGATTTAGGCCTGCAGAA+AGG + chr4_1:42633017-42633036 Msa0548530:CDS 50.0%
GTCTCTTCAACCCTTTCTGC+AGG - chr4_1:42633031-42633050 None:intergenic 50.0%
GCAGAAAGGGTTGAAGAGAC+AGG + chr4_1:42633031-42633050 Msa0548530:CDS 50.0%
GTCCTAGAGATGCTGTGTTG+AGG + chr4_1:42633230-42633249 Msa0548530:CDS 50.0%
! CTGTGTTGAGGTTGATCAGG+AGG + chr4_1:42633242-42633261 Msa0548530:CDS 50.0%
! TAGCTTGTGAAGGAGCAGAG+AGG + chr4_1:42633461-42633480 Msa0548530:CDS 50.0%
AGCTTGTGAAGGAGCAGAGA+GGG + chr4_1:42633462-42633481 Msa0548530:CDS 50.0%
AAGGAGCAGAGAGGGTTGAA+AGG + chr4_1:42633470-42633489 Msa0548530:CDS 50.0%
TGCCACTGCTTGTAAGTCTC+TGG - chr4_1:42633496-42633515 None:intergenic 50.0%
AGAGACTTACAAGCAGTGGC+AGG + chr4_1:42633495-42633514 Msa0548530:CDS 50.0%
CTTACAAGCAGTGGCAGGTT+AGG + chr4_1:42633500-42633519 Msa0548530:CDS 50.0%
!! ACTTCCATTGGCTCCAGAAC+TGG + chr4_1:42633546-42633565 Msa0548530:CDS 50.0%
GATCCGGTTAGAGATCAGCG+AGG + chr4_1:42632203-42632222 Msa0548530:CDS 55.0%
AGGCAGCATTCTTCGCCAAG+TGG + chr4_1:42632662-42632681 Msa0548530:CDS 55.0%
CATTCTTCGCCAAGTGGCGA+TGG + chr4_1:42632668-42632687 Msa0548530:CDS 55.0%
CTTGAGGCGCGATTATCTGC+TGG + chr4_1:42632725-42632744 Msa0548530:CDS 55.0%
! CGGATCTCTCTGAAAGACGC+TGG - chr4_1:42632952-42632971 None:intergenic 55.0%
CGTCTTTCAGAGAGATCCGG+TGG + chr4_1:42632953-42632972 Msa0548530:CDS 55.0%
GGCCAGAGACTTACAAGCAG+TGG + chr4_1:42633491-42633510 Msa0548530:CDS 55.0%
!! TCCATTGGCTCCAGAACTGG+TGG + chr4_1:42633549-42633568 Msa0548530:CDS 55.0%
!! TCCACCAGTTCTGGAGCCAA+TGG - chr4_1:42633553-42633572 None:intergenic 55.0%
! GATGGGAAGTGGGTTCTGCA+GGG + chr4_1:42633628-42633647 Msa0548530:CDS 55.0%
TGGGTTCTGCAGGGTTGGAA+AGG + chr4_1:42633637-42633656 Msa0548530:CDS 55.0%
GGGTTCTGCAGGGTTGGAAA+GGG + chr4_1:42633638-42633657 Msa0548530:CDS 55.0%
GATCCGGTTAGAGATCAGCG+AGG + chr4_1:42632203-42632222 Msa0548530:CDS 55.0%
AGGCAGCATTCTTCGCCAAG+TGG + chr4_1:42632662-42632681 Msa0548530:CDS 55.0%
CATTCTTCGCCAAGTGGCGA+TGG + chr4_1:42632668-42632687 Msa0548530:CDS 55.0%
CTTGAGGCGCGATTATCTGC+TGG + chr4_1:42632725-42632744 Msa0548530:CDS 55.0%
! CGGATCTCTCTGAAAGACGC+TGG - chr4_1:42632952-42632971 None:intergenic 55.0%
CGTCTTTCAGAGAGATCCGG+TGG + chr4_1:42632953-42632972 Msa0548530:CDS 55.0%
GGCCAGAGACTTACAAGCAG+TGG + chr4_1:42633491-42633510 Msa0548530:CDS 55.0%
!! TCCATTGGCTCCAGAACTGG+TGG + chr4_1:42633549-42633568 Msa0548530:CDS 55.0%
!! TCCACCAGTTCTGGAGCCAA+TGG - chr4_1:42633553-42633572 None:intergenic 55.0%
! GATGGGAAGTGGGTTCTGCA+GGG + chr4_1:42633628-42633647 Msa0548530:CDS 55.0%
TGGGTTCTGCAGGGTTGGAA+AGG + chr4_1:42633637-42633656 Msa0548530:CDS 55.0%
GGGTTCTGCAGGGTTGGAAA+GGG + chr4_1:42633638-42633657 Msa0548530:CDS 55.0%
CGCTGAAGTCCATCGCCACT+TGG - chr4_1:42632680-42632699 None:intergenic 60.0%
CTGCAGCAGACGACGACTGA+AGG - chr4_1:42632769-42632788 None:intergenic 60.0%
GCAGGCCTAAATCCTGGCTG+AGG - chr4_1:42633013-42633032 None:intergenic 60.0%
! GGATGGGAAGTGGGTTCTGC+AGG + chr4_1:42633627-42633646 Msa0548530:CDS 60.0%
! GGAAGTGGGTTCTGCAGGGT+TGG + chr4_1:42633632-42633651 Msa0548530:CDS 60.0%
CGCTGAAGTCCATCGCCACT+TGG - chr4_1:42632680-42632699 None:intergenic 60.0%
CTGCAGCAGACGACGACTGA+AGG - chr4_1:42632769-42632788 None:intergenic 60.0%
GCAGGCCTAAATCCTGGCTG+AGG - chr4_1:42633013-42633032 None:intergenic 60.0%
! GGATGGGAAGTGGGTTCTGC+AGG + chr4_1:42633627-42633646 Msa0548530:CDS 60.0%
! GGAAGTGGGTTCTGCAGGGT+TGG + chr4_1:42633632-42633651 Msa0548530:CDS 60.0%
AGAGATCCGGTGGGCCACCA+AGG + chr4_1:42632963-42632982 Msa0548530:CDS 65.0%
! ACGAAGCCTTGGTGGCCCAC+CGG - chr4_1:42632972-42632991 None:intergenic 65.0%
AGAGATCCGGTGGGCCACCA+AGG + chr4_1:42632963-42632982 Msa0548530:CDS 65.0%
! ACGAAGCCTTGGTGGCCCAC+CGG - chr4_1:42632972-42632991 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 42631741 42633699 42631741 ID=Msa0548530;Name=Msa0548530
chr4_1 mRNA 42631741 42633699 42631741 ID=Msa0548530-mRNA-1;Parent=Msa0548530;Name=Msa0548530-mRNA-1;_AED=0.35;_eAED=0.35;_QI=0|-1|0|1|-1|1|1|0|652
chr4_1 exon 42631741 42633699 42631741 ID=Msa0548530-mRNA-1:exon:11020;Parent=Msa0548530-mRNA-1
chr4_1 CDS 42631741 42633699 42631741 ID=Msa0548530-mRNA-1:cds;Parent=Msa0548530-mRNA-1
Gene Sequence

>Msa0548530

ATGTTTTCTACAGAAACAGTCGTAGAAAATTTTCCAGGTTCTGTTAACGGATGCATGTTTGGAAACATACCCGTGTCTGTTTTTTCAAACCAAAACCCAGAAAACGCGTTTAAATTCGAAGATTCAAATTCAATATCACCTTCAAGTGATTCTGTTCCTTCTTCTGGAATAACCTCAAATGGTGAATCTTCTGAGAGTAGTAAATATTCAAACCCTATTCTTAGGTATATTAGTGATATTCTTATGGATGAAGAAGATTTGGAGACAAAACCTTGTATGTTGCAAGATTGTTTGAAGCTTCAAGCTGCTGAAAAATCGTTTTATGATGTTCTGGGATCAGGAACTGGAACTAGTTCTAGTTCTGAGAGTTATAGTAATTGTAGTTATAATACTGATAATTCCGTTGTTGATTGCGATTCTCCGGAAAATAATGCATACGAGGTGTTTGATAAAATTCCTCATGATCCGGTTAGAGATCAGCGAGGTTTTTTCGGTTTTCCGAGTGATGTTTGGAAGCTGAATGAAGAGGGTAGTTTTGTTTTGGGTTCTAGAGGATTAGGGGAGAAGAGAGGTTATCTGATGAATGATAATGGTATTGGTATTTCACATGAAGAAGATCTGAGAAGTAACAAGGTTTCAGCTGTTTATTATGATGATACAGAGTTATCAGAAATGTTTGATGATGTTCTTCTTTGTAAGGATGGTAAAAGTCCGTCGATTTTTTGTGATAATTCTGAGTCGTCGCAATCACAGATTTCTGGTTCAGGAAGATCTAATGGAAAGACATCGCGATCGAAGAAAGGTTCCAGTAAGGGGAAAAAACTAAGTACTACGGTAGATTTATGGACTTTGTTATCTCAATGTGCACAAGCTGTTGGTAGCTATGATCAAAGGAATGCAAATGATATACTGAAGCAGATTAGGCAGCATTCTTCGCCAAGTGGCGATGGACTTCAGCGTTTAGCTCATTACTTTGCTGATGGACTTGAGGCGCGATTATCTGCTGGTACACCGATGTATAAACTCCTTCAGTCGTCGTCTGCTGCAGATATGTTGAGAGCTCATAAAGTTTATATTACAGCTTCTCCATTTCAAAGGATGTCGAATTTCTTGGCCAACAGAACTATTCTGAAGCTGGTAGAAAATAAAAGCAGCCTCCACATTATTGACTTTGGTATTTTCTATGGCTTTCAGTGGCCTTGTCTCATCCAGCGTCTTTCAGAGAGATCCGGTGGGCCACCAAGGCTTCGTATTACAGGAATTGATCTTCCTCAGCCAGGATTTAGGCCTGCAGAAAGGGTTGAAGAGACAGGTCGGCGACTTGTGAAGTACTGCAAGAGGTTTGGAGTTCCATTTGAGTACAATTGCCTTGCTCAGAAATGGGACACTATAAGACTTGAGGATCTCAAGATTGATAGAGAAGAAGTAACAGTGGTTAACTGCTTACACAGATTGAAGAATGTTTCTGATGAAACGGTGACGGCGAATTGTCCTAGAGATGCTGTGTTGAGGTTGATCAGGAGGATTAATCCAAACATTTTCATACATGGTGTAGTGAATGGAACTTATAACGCACCATTTTTTCTTACTAGGTTCAGAGAAGCACTATTCCATTTCTCATCATTGTTTGATATGCTTGAAGCTACGGTGCCGCGTGAAGATGATCAATATAGGCTGATGATTGAGAAAGGGTTATTCGGAAGGGATGCAGTAAATGTGATAGCTTGTGAAGGAGCAGAGAGGGTTGAAAGGCCAGAGACTTACAAGCAGTGGCAGGTTAGGAACAAGAGAGCAAGGTTCAAACAACTTCCATTGGCTCCAGAACTGGTGGATAGAGTGAAAGAAATGGTGAAGAAGGAGTATCCGAAAGATTTTGTAGTTGATGAGGATGGGAAGTGGGTTCTGCAGGGTTGGAAAGGGCGTATTCTGCTAGCTGTTTCTTGCTGGGTTCCTGCTTAA
Protein sequence

>Msa0548530

MFSTETVVENFPGSVNGCMFGNIPVSVFSNQNPENAFKFEDSNSISPSSDSVPSSGITSNGESSESSKYSNPILRYISDILMDEEDLETKPCMLQDCLKLQAAEKSFYDVLGSGTGTSSSSESYSNCSYNTDNSVVDCDSPENNAYEVFDKIPHDPVRDQRGFFGFPSDVWKLNEEGSFVLGSRGLGEKRGYLMNDNGIGISHEEDLRSNKVSAVYYDDTELSEMFDDVLLCKDGKSPSIFCDNSESSQSQISGSGRSNGKTSRSKKGSSKGKKLSTTVDLWTLLSQCAQAVGSYDQRNANDILKQIRQHSSPSGDGLQRLAHYFADGLEARLSAGTPMYKLLQSSSAADMLRAHKVYITASPFQRMSNFLANRTILKLVENKSSLHIIDFGIFYGFQWPCLIQRLSERSGGPPRLRITGIDLPQPGFRPAERVEETGRRLVKYCKRFGVPFEYNCLAQKWDTIRLEDLKIDREEVTVVNCLHRLKNVSDETVTANCPRDAVLRLIRRINPNIFIHGVVNGTYNAPFFLTRFREALFHFSSLFDMLEATVPREDDQYRLMIEKGLFGRDAVNVIACEGAERVERPETYKQWQVRNKRARFKQLPLAPELVDRVKEMVKKEYPKDFVVDEDGKWVLQGWKGRILLAVSCWVPA*