AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0548730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0548730 MtrunA17_Chr4g0032451 78.472 144 31 0 10 153 10 153 3.96e-77 226
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 56 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGACATGTCCTCTTCCAATT+TGG 0.278741 4_1:+43005102 None:intergenic
CATGAGAGAGGACGTGATTT+AGG 0.304402 4_1:+43004430 None:intergenic
CGCGAACCTCGTTCTCAAAT+AGG 0.305472 4_1:+43005035 None:intergenic
AAAATGAGCTGAATCAATTT+AGG 0.306772 4_1:+43004247 None:intergenic
ACAAGAACATATTCTTTAGA+AGG 0.321927 4_1:-43004485 Msa0548730:three_prime_UTR
CAGAGATAGAGGTAGCAAAA+TGG 0.323011 4_1:-43004342 Msa0548730:three_prime_UTR
TTACTGACCTCTTTACATAA+TGG 0.325529 4_1:+43004950 None:intergenic
GGAAGAGGACATGTCTCTTC+AGG 0.336599 4_1:-43005095 Msa0548730:CDS
ACCTCTATTGTCAATGCAAC+TGG 0.362982 4_1:+43004848 None:intergenic
TGAATTGATTGCAGCCAAAT+TGG 0.366887 4_1:-43005116 Msa0548730:CDS
CTCAGACATTGTTTAGAAAG+AGG 0.416468 4_1:-43004458 Msa0548730:three_prime_UTR
GCGAACCTCGTTCTCAAATA+GGG 0.423289 4_1:+43005036 None:intergenic
CTCGGCGCCTCTGAACAAAT+TGG 0.431988 4_1:-43006693 Msa0548730:five_prime_UTR
TCTTCAGGATTCATTGAGAA+AGG 0.435632 4_1:-43005080 Msa0548730:CDS
TGATATAATTGAGGAACAAT+CGG 0.438496 4_1:-43004879 Msa0548730:CDS
TGTATGTCGCACCTCATTCT+CGG 0.460727 4_1:+43005286 None:intergenic
AAACAACCGGAGCATATTGC+AGG 0.464852 4_1:-43004974 Msa0548730:CDS
GATATAATTGAGGAACAATC+GGG 0.473104 4_1:-43004878 Msa0548730:CDS
TGCAATAATTGAGAAACAAC+CGG 0.476453 4_1:-43004987 Msa0548730:CDS
AGAGATAGAGGTAGCAAAAT+GGG 0.478511 4_1:-43004341 Msa0548730:three_prime_UTR
CAATCCACTGAAGATATGTA+AGG 0.478649 4_1:-43004572 Msa0548730:three_prime_UTR
CAATATTGATGATATAATTG+AGG 0.489275 4_1:-43004888 Msa0548730:CDS
CATCTATTAGGATAGTAAAG+TGG 0.499249 4_1:+43004185 None:intergenic
ATTTATCGATGAATTGTACA+TGG 0.501762 4_1:-43004798 Msa0548730:CDS
AAATATGCATGCAGAGATAG+AGG 0.505997 4_1:-43004353 Msa0548730:three_prime_UTR
GTTCAACATTTCCGAGAATG+AGG 0.508451 4_1:-43005297 Msa0548730:intron
CATAGATTGCGGTCAAATTG+TGG 0.513642 4_1:-43004611 Msa0548730:exon
CACAACTCCAATTTGTTCAG+AGG 0.515988 4_1:+43006686 None:intergenic
ATCGATGAATTGTACATGGA+TGG 0.519577 4_1:-43004794 Msa0548730:CDS
TGCAGGTCCATTATGTAAAG+AGG 0.525512 4_1:-43004957 Msa0548730:CDS
GATTGCAGCCAAATTGGAAG+AGG 0.543019 4_1:-43005110 Msa0548730:CDS
ATCTATTAGGATAGTAAAGT+GGG 0.548839 4_1:+43004186 None:intergenic
TCCAGTTGCATTGACAATAG+AGG 0.563787 4_1:-43004849 Msa0548730:CDS
GCAAAATGGGTCTGATTCGT+CGG 0.564076 4_1:-43004328 Msa0548730:three_prime_UTR
AACAACAAAACTCGGTAGCT+CGG 0.568889 4_1:-43006711 Msa0548730:five_prime_UTR
TTGAGTCGAAGTGTTGGACT+CGG 0.572709 4_1:-43004273 Msa0548730:three_prime_UTR
AATGGACCTGCAATATGCTC+CGG 0.574719 4_1:+43004968 None:intergenic
TGCTATGTTGAAAGAGGAGA+CGG 0.574952 4_1:-43005011 Msa0548730:CDS
TACAGATTGAGTCGAAGTGT+TGG 0.577991 4_1:-43004279 Msa0548730:three_prime_UTR
TCGCGCTGCTATGTTGAAAG+AGG 0.578051 4_1:-43005017 Msa0548730:CDS
ACTTCATACATCTCATGACC+AGG 0.583348 4_1:+43004536 None:intergenic
AAGGTATGCTGCTTTGTATG+TGG 0.584948 4_1:-43004765 Msa0548730:CDS
TGTACATGGATGGACAAGAA+AGG 0.594997 4_1:-43004784 Msa0548730:CDS
GGGCTTAAACATGTGGCTGA+CGG 0.596336 4_1:-43004209 Msa0548730:three_prime_UTR
AATCCACTGAAGATATGTAA+GGG 0.600170 4_1:-43004571 Msa0548730:three_prime_UTR
AACTGCCCTATTTGAGAACG+AGG 0.600432 4_1:-43005041 Msa0548730:CDS
TGGGTGCAGGAACAAAATTG+AGG 0.605697 4_1:-43005340 Msa0548730:intron
ATCCACTGAAGATATGTAAG+GGG 0.622846 4_1:-43004570 Msa0548730:three_prime_UTR
TATCAAATTAAACATGAGAG+AGG 0.633734 4_1:+43004418 None:intergenic
AGACAGAAAGTCATAGATTG+CGG 0.660115 4_1:-43004622 Msa0548730:exon
CTTCATACATCTCATGACCA+GGG 0.674119 4_1:+43004537 None:intergenic
TCCACTGAAGATATGTAAGG+GGG 0.683717 4_1:-43004569 Msa0548730:three_prime_UTR
TGTATGTGGAGAAGTCTAGT+CGG 0.687076 4_1:-43004751 Msa0548730:intron
GTAAACCTAACAACAAAACT+CGG 0.690703 4_1:-43006719 Msa0548730:five_prime_UTR
ATACATGAAACTGAGAACAG+AGG 0.729964 4_1:-43004924 Msa0548730:CDS
ATATAATTGAGGAACAATCG+GGG 0.746906 4_1:-43004877 Msa0548730:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAATTTTTAAACAAAAAG+AGG + chr4_1:43004942-43004961 None:intergenic 10.0%
!! CAAATCTTCTATTTAAATTA+TGG + chr4_1:43004809-43004828 None:intergenic 15.0%
!! ATTAATAAAAGAGACATTTA+TGG + chr4_1:43005163-43005182 None:intergenic 15.0%
!! TATGCTATAAGCTTAAAATT+AGG - chr4_1:43004601-43004620 Msa0548730:three_prime_UTR 20.0%
!! AAATGAAATTCTTGAAAATG+TGG - chr4_1:43004675-43004694 Msa0548730:intron 20.0%
!!! ATCATCTAAATGATCATTTT+CGG + chr4_1:43004737-43004756 None:intergenic 20.0%
!!! TTTTGCGTTGTTATTTTATT+TGG - chr4_1:43004842-43004861 Msa0548730:CDS 20.0%
!!! TTTGCGTTGTTATTTTATTT+GGG - chr4_1:43004843-43004862 Msa0548730:CDS 20.0%
!!! GTCTCTTTTATTAATCATTT+TGG - chr4_1:43005168-43005187 Msa0548730:intron 20.0%
!! TCAAATCACTATTTAGATTT+AGG - chr4_1:43005194-43005213 Msa0548730:intron 20.0%
!!! ACTATTTAGATTTAGGATAA+TGG - chr4_1:43005201-43005220 Msa0548730:intron 20.0%
!!! CTATTTAGATTTAGGATAAT+GGG - chr4_1:43005202-43005221 Msa0548730:intron 20.0%
!! CATAAACTAATTCATATGTT+AGG - chr4_1:43005356-43005375 Msa0548730:intron 20.0%
!!! CAATATTGATGATATAATTG+AGG - chr4_1:43006033-43006052 Msa0548730:intron 20.0%
!! AAAACTGTAAGAAGTTAAAA+AGG + chr4_1:43006238-43006257 None:intergenic 20.0%
!!! GCTGTTTTAATAAGCTATAT+TGG + chr4_1:43004523-43004542 None:intergenic 25.0%
! TTATCATCAACTTATAGTCA+GGG + chr4_1:43004643-43004662 None:intergenic 25.0%
! ATTATCATCAACTTATAGTC+AGG + chr4_1:43004644-43004663 None:intergenic 25.0%
! GGTTAATACTGAAAATATAG+AGG - chr4_1:43004696-43004715 Msa0548730:intron 25.0%
!!! TTGCGTTGTTATTTTATTTG+GGG - chr4_1:43004844-43004863 Msa0548730:CDS 25.0%
!!! TTATGTTAAAAAAGTATCCC+AGG + chr4_1:43005341-43005360 None:intergenic 25.0%
!!! TTTTAAGTTTGATTAGTCTG+TGG - chr4_1:43005418-43005437 Msa0548730:intron 25.0%
! AAATATTTATTATTGGGTGC+AGG - chr4_1:43005568-43005587 Msa0548730:intron 25.0%
! TGATATAATTGAGGAACAAT+CGG - chr4_1:43006042-43006061 Msa0548730:intron 25.0%
! ATTTATCGATGAATTGTACA+TGG - chr4_1:43006123-43006142 Msa0548730:intron 25.0%
! ACAAGAACATATTCTTTAGA+AGG - chr4_1:43006436-43006455 Msa0548730:intron 25.0%
! TATCAAATTAAACATGAGAG+AGG + chr4_1:43006506-43006525 None:intergenic 25.0%
! TAAAAAATAGAGGACTAAAC+GGG + chr4_1:43006624-43006643 None:intergenic 25.0%
! GTAAAAAATAGAGGACTAAA+CGG + chr4_1:43006625-43006644 None:intergenic 25.0%
! ACTCAATCTGTAAAAAATAG+AGG + chr4_1:43006634-43006653 None:intergenic 25.0%
! AAAATGAGCTGAATCAATTT+AGG + chr4_1:43006677-43006696 None:intergenic 25.0%
GTAAACCTAACAACAAAACT+CGG - chr4_1:43004202-43004221 Msa0548730:three_prime_UTR 30.0%
CATAGATCAGGAAAGTTAAA+CGG - chr4_1:43004417-43004436 Msa0548730:three_prime_UTR 30.0%
!! TGTTGATGATATTGAAGATG+TGG - chr4_1:43004780-43004799 Msa0548730:CDS 30.0%
!! GGCATATTTTTACTGAGAAT+CGG - chr4_1:43004865-43004884 Msa0548730:CDS 30.0%
!! GCATATTTTTACTGAGAATC+GGG - chr4_1:43004866-43004885 Msa0548730:CDS 30.0%
GGCGTAAATTTAAAAGTTGA+GGG - chr4_1:43005223-43005242 Msa0548730:intron 30.0%
GCGTAAATTTAAAAGTTGAG+GGG - chr4_1:43005224-43005243 Msa0548730:intron 30.0%
CGTAAATTTAAAAGTTGAGG+GGG - chr4_1:43005225-43005244 Msa0548730:intron 30.0%
ATACAGAGGGAATTAATCAT+TGG + chr4_1:43005285-43005304 None:intergenic 30.0%
! CTTGTGGATGATTATTGATT+AGG - chr4_1:43005446-43005465 Msa0548730:intron 30.0%
! GGATGATTATTGATTAGGAT+CGG - chr4_1:43005451-43005470 Msa0548730:intron 30.0%
CTGATTTATGCACAATCTAT+TGG + chr4_1:43005540-43005559 None:intergenic 30.0%
GTGTATAAGAGAATAAGCAA+CGG - chr4_1:43005696-43005715 Msa0548730:intron 30.0%
AACTTGAATTTGGCTGTAAA+TGG - chr4_1:43005721-43005740 Msa0548730:intron 30.0%
TGCAATAATTGAGAAACAAC+CGG - chr4_1:43005934-43005953 Msa0548730:intron 30.0%
TTACTGACCTCTTTACATAA+TGG + chr4_1:43005974-43005993 None:intergenic 30.0%
GATATAATTGAGGAACAATC+GGG - chr4_1:43006043-43006062 Msa0548730:intron 30.0%
ATATAATTGAGGAACAATCG+GGG - chr4_1:43006044-43006063 Msa0548730:intron 30.0%
AATCCACTGAAGATATGTAA+GGG - chr4_1:43006350-43006369 Msa0548730:intron 30.0%
!!! GCTCATTTTGTCTTGTTTTT+TGG - chr4_1:43006688-43006707 Msa0548730:five_prime_UTR 30.0%
!!! CATTTTGTCTTGTTTTTTGG+CGG - chr4_1:43006691-43006710 Msa0548730:five_prime_UTR 30.0%
!!! ATTTTGTCTTGTTTTTTGGC+GGG - chr4_1:43006692-43006711 Msa0548730:five_prime_UTR 30.0%
GTCAATAGCATAGGTTCTAT+GGG + chr4_1:43004561-43004580 None:intergenic 35.0%
TGTCAATAGCATAGGTTCTA+TGG + chr4_1:43004562-43004581 None:intergenic 35.0%
ACATAAGCTGTCAATAGCAT+AGG + chr4_1:43004570-43004589 None:intergenic 35.0%
GCTATTGACAGCTTATGTTT+AGG - chr4_1:43004572-43004591 Msa0548730:three_prime_UTR 35.0%
TTAAACAAAAAGAGGCAGCA+AGG + chr4_1:43004934-43004953 None:intergenic 35.0%
TCCATAAATCAGTATGACAG+CGG + chr4_1:43005108-43005127 None:intergenic 35.0%
GGGCGTAAATTTAAAAGTTG+AGG - chr4_1:43005222-43005241 Msa0548730:intron 35.0%
AGTGACTACTTCAATACAGA+GGG + chr4_1:43005298-43005317 None:intergenic 35.0%
AAGTGACTACTTCAATACAG+AGG + chr4_1:43005299-43005318 None:intergenic 35.0%
TTTATACAAACCTATGCTCC+TGG - chr4_1:43005320-43005339 Msa0548730:exon 35.0%
TTATACAAACCTATGCTCCT+GGG - chr4_1:43005321-43005340 Msa0548730:exon 35.0%
! TGTTAGGTCACTTGTTGAAT+AGG - chr4_1:43005372-43005391 Msa0548730:intron 35.0%
! TGATATCCAATAGTGTTGCT+AGG - chr4_1:43005499-43005518 Msa0548730:intron 35.0%
AGCAACGGTAAACTTGAATT+TGG - chr4_1:43005711-43005730 Msa0548730:intron 35.0%
TGAATTTGGCTGTAAATGGA+TGG - chr4_1:43005725-43005744 Msa0548730:intron 35.0%
GAATTTGGCTGTAAATGGAT+GGG - chr4_1:43005726-43005745 Msa0548730:intron 35.0%
TGCTTACTAGAAACTGTTTG+TGG - chr4_1:43005768-43005787 Msa0548730:intron 35.0%
TGAATTGATTGCAGCCAAAT+TGG - chr4_1:43005805-43005824 Msa0548730:intron 35.0%
TCTTCAGGATTCATTGAGAA+AGG - chr4_1:43005841-43005860 Msa0548730:intron 35.0%
ATACATGAAACTGAGAACAG+AGG - chr4_1:43005997-43006016 Msa0548730:intron 35.0%
ATCGATGAATTGTACATGGA+TGG - chr4_1:43006127-43006146 Msa0548730:intron 35.0%
! ACAGTTTTTCTTGTCACTGT+AGG - chr4_1:43006250-43006269 Msa0548730:intron 35.0%
AGACAGAAAGTCATAGATTG+CGG - chr4_1:43006299-43006318 Msa0548730:intron 35.0%
CAATCCACTGAAGATATGTA+AGG - chr4_1:43006349-43006368 Msa0548730:intron 35.0%
ATCCACTGAAGATATGTAAG+GGG - chr4_1:43006351-43006370 Msa0548730:intron 35.0%
CTCAGACATTGTTTAGAAAG+AGG - chr4_1:43006463-43006482 Msa0548730:intron 35.0%
AAATATGCATGCAGAGATAG+AGG - chr4_1:43006568-43006587 Msa0548730:intron 35.0%
AGAGATAGAGGTAGCAAAAT+GGG - chr4_1:43006580-43006599 Msa0548730:intron 35.0%
!!! AGCTACCGAGTTTTGTTGTT+AGG + chr4_1:43004210-43004229 None:intergenic 40.0%
AACAACAAAACTCGGTAGCT+CGG - chr4_1:43004210-43004229 Msa0548730:three_prime_UTR 40.0%
! CACAACTCCAATTTGTTCAG+AGG + chr4_1:43004238-43004257 None:intergenic 40.0%
CATCATCATCATCATCATCC+AGG - chr4_1:43004265-43004284 Msa0548730:three_prime_UTR 40.0%
CGAACAGAAAAAGAGAGAAG+GGG + chr4_1:43004302-43004321 None:intergenic 40.0%
CCGAACAGAAAAAGAGAGAA+GGG + chr4_1:43004303-43004322 None:intergenic 40.0%
! CCCTTCTCTCTTTTTCTGTT+CGG - chr4_1:43004300-43004319 Msa0548730:three_prime_UTR 40.0%
TCCGAACAGAAAAAGAGAGA+AGG + chr4_1:43004304-43004323 None:intergenic 40.0%
TTCTTTCCCCTTTCTCGATA+CGG - chr4_1:43004326-43004345 Msa0548730:three_prime_UTR 40.0%
ACTTATAGTCAGGGTGTGTA+TGG + chr4_1:43004634-43004653 None:intergenic 40.0%
AATCGGGTTTCCAGTTTGAT+AGG - chr4_1:43004882-43004901 Msa0548730:CDS 40.0%
ATCGGGTTTCCAGTTTGATA+GGG - chr4_1:43004883-43004902 Msa0548730:CDS 40.0%
!! TCCGCTGTCATACTGATTTA+TGG - chr4_1:43005104-43005123 Msa0548730:CDS 40.0%
!! AAAAAGTATCCCAGGAGCAT+AGG + chr4_1:43005333-43005352 None:intergenic 40.0%
TTAGTCTGTGGATGTTCTTG+TGG - chr4_1:43005430-43005449 Msa0548730:intron 40.0%
CAAAAGCCTAGCAACACTAT+TGG + chr4_1:43005508-43005527 None:intergenic 40.0%
!! CAATAGTGTTGCTAGGCTTT+TGG - chr4_1:43005506-43005525 Msa0548730:intron 40.0%
GTTCAACATTTCCGAGAATG+AGG - chr4_1:43005624-43005643 Msa0548730:intron 40.0%
AGACATGTCCTCTTCCAATT+TGG + chr4_1:43005822-43005841 None:intergenic 40.0%
TGCTATGTTGAAAGAGGAGA+CGG - chr4_1:43005910-43005929 Msa0548730:intron 40.0%
TGCAGGTCCATTATGTAAAG+AGG - chr4_1:43005964-43005983 Msa0548730:intron 40.0%
! TCCAGTTGCATTGACAATAG+AGG - chr4_1:43006072-43006091 Msa0548730:intron 40.0%
ACCTCTATTGTCAATGCAAC+TGG + chr4_1:43006076-43006095 None:intergenic 40.0%
TGTACATGGATGGACAAGAA+AGG - chr4_1:43006137-43006156 Msa0548730:intron 40.0%
!! AAGGTATGCTGCTTTGTATG+TGG - chr4_1:43006156-43006175 Msa0548730:intron 40.0%
TGTATGTGGAGAAGTCTAGT+CGG - chr4_1:43006170-43006189 Msa0548730:intron 40.0%
CTAGTCGGTAAGTTATACAC+TGG - chr4_1:43006185-43006204 Msa0548730:intron 40.0%
CATAGATTGCGGTCAAATTG+TGG - chr4_1:43006310-43006329 Msa0548730:intron 40.0%
TCCACTGAAGATATGTAAGG+GGG - chr4_1:43006352-43006371 Msa0548730:intron 40.0%
TAAGGGGGTAAAAAAAACCC+TGG - chr4_1:43006367-43006386 Msa0548730:intron 40.0%
CTTCATACATCTCATGACCA+GGG + chr4_1:43006387-43006406 None:intergenic 40.0%
ACTTCATACATCTCATGACC+AGG + chr4_1:43006388-43006407 None:intergenic 40.0%
CAGAGATAGAGGTAGCAAAA+TGG - chr4_1:43006579-43006598 Msa0548730:intron 40.0%
TACAGATTGAGTCGAAGTGT+TGG - chr4_1:43006642-43006661 Msa0548730:intron 40.0%
GAAGGGGAAACAAACAAACC+TGG + chr4_1:43004286-43004305 None:intergenic 45.0%
CGCGATTCACAACATAGATC+AGG - chr4_1:43004405-43004424 Msa0548730:three_prime_UTR 45.0%
AAGAGTCGACCCTATCAAAC+TGG + chr4_1:43004895-43004914 None:intergenic 45.0%
!! GATAGGGTCGACTCTTTTGA+AGG - chr4_1:43004899-43004918 Msa0548730:CDS 45.0%
GACAGCGGAGTATACAATCA+GGG + chr4_1:43005093-43005112 None:intergenic 45.0%
TGACAGCGGAGTATACAATC+AGG + chr4_1:43005094-43005113 None:intergenic 45.0%
TGAATCCCCTGGTTAGTAGA+TGG - chr4_1:43005254-43005273 Msa0548730:intron 45.0%
TGGGTGCAGGAACAAAATTG+AGG - chr4_1:43005581-43005600 Msa0548730:intron 45.0%
TGTATGTCGCACCTCATTCT+CGG + chr4_1:43005638-43005657 None:intergenic 45.0%
GATTGCAGCCAAATTGGAAG+AGG - chr4_1:43005811-43005830 Msa0548730:intron 45.0%
AACTGCCCTATTTGAGAACG+AGG - chr4_1:43005880-43005899 Msa0548730:intron 45.0%
GCGAACCTCGTTCTCAAATA+GGG + chr4_1:43005888-43005907 None:intergenic 45.0%
AAACAACCGGAGCATATTGC+AGG - chr4_1:43005947-43005966 Msa0548730:intron 45.0%
AATGGACCTGCAATATGCTC+CGG + chr4_1:43005956-43005975 None:intergenic 45.0%
ACCCCCTTACATATCTTCAG+TGG + chr4_1:43006356-43006375 None:intergenic 45.0%
CATGAGAGAGGACGTGATTT+AGG + chr4_1:43006494-43006513 None:intergenic 45.0%
! GCAAAATGGGTCTGATTCGT+CGG - chr4_1:43006593-43006612 Msa0548730:intron 45.0%
!! TTGAGTCGAAGTGTTGGACT+CGG - chr4_1:43006648-43006667 Msa0548730:intron 45.0%
!!! TTTTGGCGGGCTTAAACATG+TGG - chr4_1:43006705-43006724 Msa0548730:five_prime_UTR 45.0%
!! TTATTCTAAATATTTATTAT+TGG - chr4_1:43005561-43005580 Msa0548730:intron 5.0%
!! TATTCTAAATATTTATTATT+GGG - chr4_1:43005562-43005581 Msa0548730:intron 5.0%
TTTCCCCTTTCTCGATACGG+CGG - chr4_1:43004329-43004348 Msa0548730:three_prime_UTR 50.0%
TGAATCGCGCCGAATTGAAG+GGG + chr4_1:43004394-43004413 None:intergenic 50.0%
GTGAATCGCGCCGAATTGAA+GGG + chr4_1:43004395-43004414 None:intergenic 50.0%
TGTGAATCGCGCCGAATTGA+AGG + chr4_1:43004396-43004415 None:intergenic 50.0%
CGGTTGAGTAGACGACTGTT+TGG - chr4_1:43004437-43004456 Msa0548730:three_prime_UTR 50.0%
!! AGGGTCGACTCTTTTGAAGG+AGG - chr4_1:43004902-43004921 Msa0548730:CDS 50.0%
CAGAGCCATCTACTAACCAG+GGG + chr4_1:43005262-43005281 None:intergenic 50.0%
GCAGAGCCATCTACTAACCA+GGG + chr4_1:43005263-43005282 None:intergenic 50.0%
GGAAGAGGACATGTCTCTTC+AGG - chr4_1:43005826-43005845 Msa0548730:intron 50.0%
CGCGAACCTCGTTCTCAAAT+AGG + chr4_1:43005889-43005908 None:intergenic 50.0%
TCGCGCTGCTATGTTGAAAG+AGG - chr4_1:43005904-43005923 Msa0548730:intron 50.0%
GGGCTTAAACATGTGGCTGA+CGG - chr4_1:43006712-43006731 Msa0548730:five_prime_UTR 50.0%
CTCGGCGCCTCTGAACAAAT+TGG - chr4_1:43004228-43004247 Msa0548730:three_prime_UTR 55.0%
CGAGTATGCGATTCCATCGC+TGG - chr4_1:43004356-43004375 Msa0548730:three_prime_UTR 55.0%
CCGAATTGAAGGGGCGGATA+CGG + chr4_1:43004385-43004404 None:intergenic 55.0%
CCGTATCCGCCCCTTCAATT+CGG - chr4_1:43004382-43004401 Msa0548730:three_prime_UTR 55.0%
GGCAGAGCCATCTACTAACC+AGG + chr4_1:43005264-43005283 None:intergenic 55.0%
TGCGCCGCCGTATCGAGAAA+GGG + chr4_1:43004336-43004355 None:intergenic 60.0%
ATCGCGCCGAATTGAAGGGG+CGG + chr4_1:43004391-43004410 None:intergenic 60.0%
GCGCCGCCGTATCGAGAAAG+GGG + chr4_1:43004335-43004354 None:intergenic 65.0%
GTGCGCCGCCGTATCGAGAA+AGG + chr4_1:43004337-43004356 None:intergenic 65.0%
GGGGGTTCGACTGAATCCCC+TGG - chr4_1:43005243-43005262 Msa0548730:intron 65.0%
GCGGATACGGCAGCCAGCGA+TGG + chr4_1:43004372-43004391 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 43004192 43006751 43004192 ID=Msa0548730;Name=Msa0548730
chr4_1 mRNA 43004192 43006751 43004192 ID=Msa0548730-mRNA-1;Parent=Msa0548730;Name=Msa0548730-mRNA-1;_AED=0.02;_eAED=0.02;_QI=124|1|1|1|0.66|0.75|4|437|154
chr4_1 exon 43004192 43004671 43004192 ID=Msa0548730-mRNA-1:exon:11157;Parent=Msa0548730-mRNA-1
chr4_1 exon 43004752 43005146 43004752 ID=Msa0548730-mRNA-1:exon:11156;Parent=Msa0548730-mRNA-1
chr4_1 exon 43005298 43005353 43005298 ID=Msa0548730-mRNA-1:exon:11155;Parent=Msa0548730-mRNA-1
chr4_1 exon 43006657 43006751 43006657 ID=Msa0548730-mRNA-1:exon:11154;Parent=Msa0548730-mRNA-1
chr4_1 five_prime_UTR 43006657 43006751 43006657 ID=Msa0548730-mRNA-1:five_prime_utr;Parent=Msa0548730-mRNA-1
chr4_1 five_prime_UTR 43005325 43005353 43005325 ID=Msa0548730-mRNA-1:five_prime_utr;Parent=Msa0548730-mRNA-1
chr4_1 CDS 43005298 43005324 43005298 ID=Msa0548730-mRNA-1:cds;Parent=Msa0548730-mRNA-1
chr4_1 CDS 43004752 43005146 43004752 ID=Msa0548730-mRNA-1:cds;Parent=Msa0548730-mRNA-1
chr4_1 CDS 43004629 43004671 43004629 ID=Msa0548730-mRNA-1:cds;Parent=Msa0548730-mRNA-1
chr4_1 three_prime_UTR 43004192 43004628 43004192 ID=Msa0548730-mRNA-1:three_prime_utr;Parent=Msa0548730-mRNA-1
Gene Sequence

>Msa0548730

ATGAAGTTCAACATTTCCGAGAATGAGCAAATTGATGAATTGATTGCAGCCAAATTGGAAGAGGACATGTCTCTTCAGGATTCATTGAGAAAGGTTCATGACTTGTTTATAACTGCCCTATTTGAGAACGAGGTTCGCGCTGCTATGTTGAAAGAGGAGACGGATGCAATAATTGAGAAACAACCGGAGCATATTGCAGGTCCATTATGTAAAGAGGTCAGTAAAATATACATGAAACTGAGAACAGAGGTCAGTGAATTCTTCAATATTGATGATATAATTGAGGAACAATCGGGGCGTGTTCCAGTTGCATTGACAATAGAGGTCATTAAAACGAACATGAAATTGCGAAAATTTATCGATGAATTGTACATGGATGGACAAGAAAGGTATGCTGCTTTGTATGTGGAGAAGTCTAGTCGGTTAATCAAAGACAAAACAGAAAAGCAAGACAGAAAGTCATAG
Protein sequence

>Msa0548730

MKFNISENEQIDELIAAKLEEDMSLQDSLRKVHDLFITALFENEVRAAMLKEETDAIIEKQPEHIAGPLCKEVSKIYMKLRTEVSEFFNIDDIIEEQSGRVPVALTIEVIKTNMKLRKFIDELYMDGQERYAALYVEKSSRLIKDKTEKQDRKS*