AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0684580

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0684580 MtrunA17_Chr4g0032451 78.472 144 31 0 10 153 10 153 3.96e-77 226
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 60 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATTCTAAATATTTATTAT+TGG 0.235823 4_4:-47949334 Msa0684580:five_prime_UTR
CAATAGTGTTGCTAGGCTTT+TGG 0.236556 4_4:-47949389 Msa0684580:five_prime_UTR
TATTCTAAATATTTATTATT+GGG 0.266469 4_4:-47949333 Msa0684580:five_prime_UTR
CTGATTTATGCACAATCTAT+TGG 0.274318 4_4:+47949358 None:intergenic
AGACATATCCTCTTCCAATT+TGG 0.292212 4_4:+47949076 None:intergenic
CATGAGAGAGGACGTGATTT+AGG 0.304402 4_4:+47948404 None:intergenic
CGCGAACCTCGTTCTCAAAT+AGG 0.305472 4_4:+47949009 None:intergenic
AAAATGAGCTGAATCAATTT+AGG 0.306772 4_4:+47948221 None:intergenic
ACAAGAACATATTCTTTAGA+AGG 0.321927 4_4:-47948459 Msa0684580:three_prime_UTR
CAGAGATAGAGGTAGCAAAA+TGG 0.323011 4_4:-47948316 Msa0684580:three_prime_UTR
TTACTGACCTCTTTACATAA+TGG 0.325529 4_4:+47948924 None:intergenic
GGAAGAGGATATGTCTCTTC+AGG 0.339040 4_4:-47949069 Msa0684580:CDS
ACCTCTATTGTCAATGCAAC+TGG 0.362982 4_4:+47948822 None:intergenic
TGAATTGATTGCAGCCAAAT+TGG 0.366887 4_4:-47949090 Msa0684580:CDS
CTCAGACATTGTTTAGAAAG+AGG 0.416468 4_4:-47948432 Msa0684580:three_prime_UTR
GCGAACCTCGTTCTCAAATA+GGG 0.423289 4_4:+47949010 None:intergenic
TCTTCAGGATTCATTGAGAA+AGG 0.435632 4_4:-47949054 Msa0684580:CDS
TGATATAATTGAGGAACAAT+CGG 0.438496 4_4:-47948853 Msa0684580:CDS
CAAAAGCCTAGCAACACTAT+TGG 0.456467 4_4:+47949390 None:intergenic
TGTATGTCGCACCTCATTCT+CGG 0.460727 4_4:+47949260 None:intergenic
AAACAACCGGAGCATATTGC+AGG 0.464852 4_4:-47948948 Msa0684580:CDS
AAATATTTATTATTGGGTGC+AGG 0.466260 4_4:-47949327 Msa0684580:five_prime_UTR
GATATAATTGAGGAACAATC+GGG 0.473104 4_4:-47948852 Msa0684580:CDS
TGCAATAATTGAGAAACAAC+CGG 0.476453 4_4:-47948961 Msa0684580:CDS
AGAGATAGAGGTAGCAAAAT+GGG 0.478511 4_4:-47948315 Msa0684580:three_prime_UTR
CAATCCACTGAAGATATGTA+AGG 0.478649 4_4:-47948546 Msa0684580:three_prime_UTR
CAATATTGATGATATAATTG+AGG 0.489275 4_4:-47948862 Msa0684580:CDS
CATCTATTAGGATAGTAAAG+TGG 0.499249 4_4:+47948159 None:intergenic
ATTTATCGATGAATTGTACA+TGG 0.501762 4_4:-47948772 Msa0684580:CDS
AAATATGCATGCAGAGATAG+AGG 0.505997 4_4:-47948327 Msa0684580:three_prime_UTR
GTTCAACATTTCCGAGAATG+AGG 0.508451 4_4:-47949271 Msa0684580:intron
CATAGATTGCGGTCAAATTG+TGG 0.513642 4_4:-47948585 Msa0684580:exon
ATCGATGAATTGTACATGGA+TGG 0.519577 4_4:-47948768 Msa0684580:CDS
GATTGCAGCCAAATTGGAAG+AGG 0.525218 4_4:-47949084 Msa0684580:CDS
TGCAGGTCCATTATGTAAAG+AGG 0.525512 4_4:-47948931 Msa0684580:CDS
GGATGATTATTGATTAGGAT+CGG 0.533324 4_4:-47949444 None:intergenic
ATCTATTAGGATAGTAAAGT+GGG 0.548839 4_4:+47948160 None:intergenic
TGATATCCAATAGTGTTGCT+AGG 0.557729 4_4:-47949396 Msa0684580:five_prime_UTR
TCCAGTTGCATTGACAATAG+AGG 0.563787 4_4:-47948823 Msa0684580:CDS
GCAAAATGGGTCTGATTCGT+CGG 0.564076 4_4:-47948302 Msa0684580:three_prime_UTR
TTGAGTCGAAGTGTTGGACT+CGG 0.572709 4_4:-47948247 Msa0684580:three_prime_UTR
AATGGACCTGCAATATGCTC+CGG 0.574719 4_4:+47948942 None:intergenic
TGCTATGTTGAAAGAGGAGA+CGG 0.574952 4_4:-47948985 Msa0684580:CDS
TACAGATTGAGTCGAAGTGT+TGG 0.577991 4_4:-47948253 Msa0684580:three_prime_UTR
TCGCGCTGCTATGTTGAAAG+AGG 0.578051 4_4:-47948991 Msa0684580:CDS
ACTTCATACATCTCATGACC+AGG 0.583348 4_4:+47948510 None:intergenic
AAGGTATGCTGCTTTGTATG+TGG 0.584948 4_4:-47948739 Msa0684580:CDS
TGTACATGGATGGACAAGAA+AGG 0.594997 4_4:-47948758 Msa0684580:CDS
GGGCTTAAACATGTGGCTGA+CGG 0.596336 4_4:-47948183 Msa0684580:three_prime_UTR
AATCCACTGAAGATATGTAA+GGG 0.600170 4_4:-47948545 Msa0684580:three_prime_UTR
AACTGCCCTATTTGAGAACG+AGG 0.600432 4_4:-47949015 Msa0684580:CDS
TGGGTGCAGGAACAAAATTG+AGG 0.605697 4_4:-47949314 Msa0684580:five_prime_UTR
ATCCACTGAAGATATGTAAG+GGG 0.622846 4_4:-47948544 Msa0684580:three_prime_UTR
TATCAAATTAAACATGAGAG+AGG 0.633734 4_4:+47948392 None:intergenic
AGACAGAAAGTCATAGATTG+CGG 0.660115 4_4:-47948596 Msa0684580:exon
CTTCATACATCTCATGACCA+GGG 0.674119 4_4:+47948511 None:intergenic
TCCACTGAAGATATGTAAGG+GGG 0.683717 4_4:-47948543 Msa0684580:three_prime_UTR
TGTATGTGGAGAAGTCTAGT+CGG 0.687076 4_4:-47948725 Msa0684580:intron
ATACATGAAACTGAGAACAG+AGG 0.729964 4_4:-47948898 Msa0684580:CDS
ATATAATTGAGGAACAATCG+GGG 0.746906 4_4:-47948851 Msa0684580:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAATATTGATGATATAATTG+AGG - chr4_4:47948737-47948756 Msa0684580:CDS 20.0%
!! AAAACTGTAAGAAGTTAAAA+AGG + chr4_4:47948942-47948961 None:intergenic 20.0%
!!! CAATATTGATGATATAATTG+AGG - chr4_4:47948737-47948756 Msa0684580:CDS 20.0%
!! AAAACTGTAAGAAGTTAAAA+AGG + chr4_4:47948942-47948961 None:intergenic 20.0%
! AAATATTTATTATTGGGTGC+AGG - chr4_4:47948272-47948291 Msa0684580:three_prime_UTR 25.0%
! TGATATAATTGAGGAACAAT+CGG - chr4_4:47948746-47948765 Msa0684580:CDS 25.0%
! ATTTATCGATGAATTGTACA+TGG - chr4_4:47948827-47948846 Msa0684580:CDS 25.0%
! ACAAGAACATATTCTTTAGA+AGG - chr4_4:47949140-47949159 Msa0684580:intron 25.0%
! TATCAAATTAAACATGAGAG+AGG + chr4_4:47949210-47949229 None:intergenic 25.0%
! TAAAAAATAGAGGACTAAAC+GGG + chr4_4:47949328-47949347 None:intergenic 25.0%
! GTAAAAAATAGAGGACTAAA+CGG + chr4_4:47949329-47949348 None:intergenic 25.0%
! ACTCAATCTGTAAAAAATAG+AGG + chr4_4:47949338-47949357 None:intergenic 25.0%
! AAAATGAGCTGAATCAATTT+AGG + chr4_4:47949381-47949400 None:intergenic 25.0%
! AAATATTTATTATTGGGTGC+AGG - chr4_4:47948272-47948291 Msa0684580:three_prime_UTR 25.0%
! TGATATAATTGAGGAACAAT+CGG - chr4_4:47948746-47948765 Msa0684580:CDS 25.0%
! ATTTATCGATGAATTGTACA+TGG - chr4_4:47948827-47948846 Msa0684580:CDS 25.0%
! ACAAGAACATATTCTTTAGA+AGG - chr4_4:47949140-47949159 Msa0684580:intron 25.0%
! TATCAAATTAAACATGAGAG+AGG + chr4_4:47949210-47949229 None:intergenic 25.0%
! TAAAAAATAGAGGACTAAAC+GGG + chr4_4:47949328-47949347 None:intergenic 25.0%
! GTAAAAAATAGAGGACTAAA+CGG + chr4_4:47949329-47949348 None:intergenic 25.0%
! ACTCAATCTGTAAAAAATAG+AGG + chr4_4:47949338-47949357 None:intergenic 25.0%
! AAAATGAGCTGAATCAATTT+AGG + chr4_4:47949381-47949400 None:intergenic 25.0%
CTGATTTATGCACAATCTAT+TGG + chr4_4:47948244-47948263 None:intergenic 30.0%
GTGTATAAGAGAATAAGCAA+CGG - chr4_4:47948400-47948419 Msa0684580:three_prime_UTR 30.0%
AACTTGAATTTGGCTGTAAA+TGG - chr4_4:47948425-47948444 Msa0684580:three_prime_UTR 30.0%
TGCAATAATTGAGAAACAAC+CGG - chr4_4:47948638-47948657 Msa0684580:intron 30.0%
TTACTGACCTCTTTACATAA+TGG + chr4_4:47948678-47948697 None:intergenic 30.0%
GATATAATTGAGGAACAATC+GGG - chr4_4:47948747-47948766 Msa0684580:CDS 30.0%
ATATAATTGAGGAACAATCG+GGG - chr4_4:47948748-47948767 Msa0684580:CDS 30.0%
AATCCACTGAAGATATGTAA+GGG - chr4_4:47949054-47949073 Msa0684580:CDS 30.0%
!!! GCTCATTTTGTCTTGTTTTT+TGG - chr4_4:47949392-47949411 Msa0684580:five_prime_UTR 30.0%
!!! CATTTTGTCTTGTTTTTTGG+CGG - chr4_4:47949395-47949414 Msa0684580:five_prime_UTR 30.0%
!!! ATTTTGTCTTGTTTTTTGGC+GGG - chr4_4:47949396-47949415 Msa0684580:five_prime_UTR 30.0%
CTGATTTATGCACAATCTAT+TGG + chr4_4:47948244-47948263 None:intergenic 30.0%
GTGTATAAGAGAATAAGCAA+CGG - chr4_4:47948400-47948419 Msa0684580:three_prime_UTR 30.0%
AACTTGAATTTGGCTGTAAA+TGG - chr4_4:47948425-47948444 Msa0684580:three_prime_UTR 30.0%
TGCAATAATTGAGAAACAAC+CGG - chr4_4:47948638-47948657 Msa0684580:intron 30.0%
TTACTGACCTCTTTACATAA+TGG + chr4_4:47948678-47948697 None:intergenic 30.0%
GATATAATTGAGGAACAATC+GGG - chr4_4:47948747-47948766 Msa0684580:CDS 30.0%
ATATAATTGAGGAACAATCG+GGG - chr4_4:47948748-47948767 Msa0684580:CDS 30.0%
AATCCACTGAAGATATGTAA+GGG - chr4_4:47949054-47949073 Msa0684580:CDS 30.0%
!!! GCTCATTTTGTCTTGTTTTT+TGG - chr4_4:47949392-47949411 Msa0684580:five_prime_UTR 30.0%
!!! CATTTTGTCTTGTTTTTTGG+CGG - chr4_4:47949395-47949414 Msa0684580:five_prime_UTR 30.0%
!!! ATTTTGTCTTGTTTTTTGGC+GGG - chr4_4:47949396-47949415 Msa0684580:five_prime_UTR 30.0%
! TGATATCCAATAGTGTTGCT+AGG - chr4_4:47948203-47948222 Msa0684580:three_prime_UTR 35.0%
AGCAACGGTAAACTTGAATT+TGG - chr4_4:47948415-47948434 Msa0684580:three_prime_UTR 35.0%
TGAATTTGGCTGTAAATGGA+TGG - chr4_4:47948429-47948448 Msa0684580:three_prime_UTR 35.0%
GAATTTGGCTGTAAATGGAT+GGG - chr4_4:47948430-47948449 Msa0684580:three_prime_UTR 35.0%
TGCTTACTAGAAACTGTTTG+TGG - chr4_4:47948472-47948491 Msa0684580:three_prime_UTR 35.0%
TGAATTGATTGCAGCCAAAT+TGG - chr4_4:47948509-47948528 Msa0684580:three_prime_UTR 35.0%
AGACATATCCTCTTCCAATT+TGG + chr4_4:47948526-47948545 None:intergenic 35.0%
TCTTCAGGATTCATTGAGAA+AGG - chr4_4:47948545-47948564 Msa0684580:three_prime_UTR 35.0%
ATACATGAAACTGAGAACAG+AGG - chr4_4:47948701-47948720 Msa0684580:intron 35.0%
ATCGATGAATTGTACATGGA+TGG - chr4_4:47948831-47948850 Msa0684580:CDS 35.0%
! ACAGTTTTTCTTGTCACTGT+AGG - chr4_4:47948954-47948973 Msa0684580:CDS 35.0%
AGACAGAAAGTCATAGATTG+CGG - chr4_4:47949003-47949022 Msa0684580:CDS 35.0%
CAATCCACTGAAGATATGTA+AGG - chr4_4:47949053-47949072 Msa0684580:CDS 35.0%
ATCCACTGAAGATATGTAAG+GGG - chr4_4:47949055-47949074 Msa0684580:CDS 35.0%
CTCAGACATTGTTTAGAAAG+AGG - chr4_4:47949167-47949186 Msa0684580:intron 35.0%
AAATATGCATGCAGAGATAG+AGG - chr4_4:47949272-47949291 Msa0684580:CDS 35.0%
AGAGATAGAGGTAGCAAAAT+GGG - chr4_4:47949284-47949303 Msa0684580:exon 35.0%
! TGATATCCAATAGTGTTGCT+AGG - chr4_4:47948203-47948222 Msa0684580:three_prime_UTR 35.0%
AGCAACGGTAAACTTGAATT+TGG - chr4_4:47948415-47948434 Msa0684580:three_prime_UTR 35.0%
TGAATTTGGCTGTAAATGGA+TGG - chr4_4:47948429-47948448 Msa0684580:three_prime_UTR 35.0%
GAATTTGGCTGTAAATGGAT+GGG - chr4_4:47948430-47948449 Msa0684580:three_prime_UTR 35.0%
TGCTTACTAGAAACTGTTTG+TGG - chr4_4:47948472-47948491 Msa0684580:three_prime_UTR 35.0%
TGAATTGATTGCAGCCAAAT+TGG - chr4_4:47948509-47948528 Msa0684580:three_prime_UTR 35.0%
AGACATATCCTCTTCCAATT+TGG + chr4_4:47948526-47948545 None:intergenic 35.0%
TCTTCAGGATTCATTGAGAA+AGG - chr4_4:47948545-47948564 Msa0684580:three_prime_UTR 35.0%
ATACATGAAACTGAGAACAG+AGG - chr4_4:47948701-47948720 Msa0684580:intron 35.0%
ATCGATGAATTGTACATGGA+TGG - chr4_4:47948831-47948850 Msa0684580:CDS 35.0%
! ACAGTTTTTCTTGTCACTGT+AGG - chr4_4:47948954-47948973 Msa0684580:CDS 35.0%
AGACAGAAAGTCATAGATTG+CGG - chr4_4:47949003-47949022 Msa0684580:CDS 35.0%
CAATCCACTGAAGATATGTA+AGG - chr4_4:47949053-47949072 Msa0684580:CDS 35.0%
ATCCACTGAAGATATGTAAG+GGG - chr4_4:47949055-47949074 Msa0684580:CDS 35.0%
CTCAGACATTGTTTAGAAAG+AGG - chr4_4:47949167-47949186 Msa0684580:intron 35.0%
AAATATGCATGCAGAGATAG+AGG - chr4_4:47949272-47949291 Msa0684580:CDS 35.0%
AGAGATAGAGGTAGCAAAAT+GGG - chr4_4:47949284-47949303 Msa0684580:exon 35.0%
CAAAAGCCTAGCAACACTAT+TGG + chr4_4:47948212-47948231 None:intergenic 40.0%
!! CAATAGTGTTGCTAGGCTTT+TGG - chr4_4:47948210-47948229 Msa0684580:three_prime_UTR 40.0%
GTTCAACATTTCCGAGAATG+AGG - chr4_4:47948328-47948347 Msa0684580:three_prime_UTR 40.0%
TGCTATGTTGAAAGAGGAGA+CGG - chr4_4:47948614-47948633 Msa0684580:CDS 40.0%
TGCAGGTCCATTATGTAAAG+AGG - chr4_4:47948668-47948687 Msa0684580:intron 40.0%
! TCCAGTTGCATTGACAATAG+AGG - chr4_4:47948776-47948795 Msa0684580:CDS 40.0%
ACCTCTATTGTCAATGCAAC+TGG + chr4_4:47948780-47948799 None:intergenic 40.0%
TGTACATGGATGGACAAGAA+AGG - chr4_4:47948841-47948860 Msa0684580:CDS 40.0%
!! AAGGTATGCTGCTTTGTATG+TGG - chr4_4:47948860-47948879 Msa0684580:CDS 40.0%
TGTATGTGGAGAAGTCTAGT+CGG - chr4_4:47948874-47948893 Msa0684580:CDS 40.0%
CTAGTCGGTAAGTTATACAC+TGG - chr4_4:47948889-47948908 Msa0684580:CDS 40.0%
CATAGATTGCGGTCAAATTG+TGG - chr4_4:47949014-47949033 Msa0684580:CDS 40.0%
TCCACTGAAGATATGTAAGG+GGG - chr4_4:47949056-47949075 Msa0684580:CDS 40.0%
TAAGGGGGTAAAAAAAACCC+TGG - chr4_4:47949071-47949090 Msa0684580:CDS 40.0%
CTTCATACATCTCATGACCA+GGG + chr4_4:47949091-47949110 None:intergenic 40.0%
ACTTCATACATCTCATGACC+AGG + chr4_4:47949092-47949111 None:intergenic 40.0%
CAGAGATAGAGGTAGCAAAA+TGG - chr4_4:47949283-47949302 Msa0684580:exon 40.0%
TACAGATTGAGTCGAAGTGT+TGG - chr4_4:47949346-47949365 Msa0684580:five_prime_UTR 40.0%
CAAAAGCCTAGCAACACTAT+TGG + chr4_4:47948212-47948231 None:intergenic 40.0%
!! CAATAGTGTTGCTAGGCTTT+TGG - chr4_4:47948210-47948229 Msa0684580:three_prime_UTR 40.0%
GTTCAACATTTCCGAGAATG+AGG - chr4_4:47948328-47948347 Msa0684580:three_prime_UTR 40.0%
TGCTATGTTGAAAGAGGAGA+CGG - chr4_4:47948614-47948633 Msa0684580:CDS 40.0%
TGCAGGTCCATTATGTAAAG+AGG - chr4_4:47948668-47948687 Msa0684580:intron 40.0%
! TCCAGTTGCATTGACAATAG+AGG - chr4_4:47948776-47948795 Msa0684580:CDS 40.0%
ACCTCTATTGTCAATGCAAC+TGG + chr4_4:47948780-47948799 None:intergenic 40.0%
TGTACATGGATGGACAAGAA+AGG - chr4_4:47948841-47948860 Msa0684580:CDS 40.0%
!! AAGGTATGCTGCTTTGTATG+TGG - chr4_4:47948860-47948879 Msa0684580:CDS 40.0%
TGTATGTGGAGAAGTCTAGT+CGG - chr4_4:47948874-47948893 Msa0684580:CDS 40.0%
CTAGTCGGTAAGTTATACAC+TGG - chr4_4:47948889-47948908 Msa0684580:CDS 40.0%
CATAGATTGCGGTCAAATTG+TGG - chr4_4:47949014-47949033 Msa0684580:CDS 40.0%
TCCACTGAAGATATGTAAGG+GGG - chr4_4:47949056-47949075 Msa0684580:CDS 40.0%
TAAGGGGGTAAAAAAAACCC+TGG - chr4_4:47949071-47949090 Msa0684580:CDS 40.0%
CTTCATACATCTCATGACCA+GGG + chr4_4:47949091-47949110 None:intergenic 40.0%
ACTTCATACATCTCATGACC+AGG + chr4_4:47949092-47949111 None:intergenic 40.0%
CAGAGATAGAGGTAGCAAAA+TGG - chr4_4:47949283-47949302 Msa0684580:exon 40.0%
TACAGATTGAGTCGAAGTGT+TGG - chr4_4:47949346-47949365 Msa0684580:five_prime_UTR 40.0%
TGGGTGCAGGAACAAAATTG+AGG - chr4_4:47948285-47948304 Msa0684580:three_prime_UTR 45.0%
TGTATGTCGCACCTCATTCT+CGG + chr4_4:47948342-47948361 None:intergenic 45.0%
GATTGCAGCCAAATTGGAAG+AGG - chr4_4:47948515-47948534 Msa0684580:three_prime_UTR 45.0%
GGAAGAGGATATGTCTCTTC+AGG - chr4_4:47948530-47948549 Msa0684580:three_prime_UTR 45.0%
AACTGCCCTATTTGAGAACG+AGG - chr4_4:47948584-47948603 Msa0684580:exon 45.0%
GCGAACCTCGTTCTCAAATA+GGG + chr4_4:47948592-47948611 None:intergenic 45.0%
AAACAACCGGAGCATATTGC+AGG - chr4_4:47948651-47948670 Msa0684580:intron 45.0%
AATGGACCTGCAATATGCTC+CGG + chr4_4:47948660-47948679 None:intergenic 45.0%
ACCCCCTTACATATCTTCAG+TGG + chr4_4:47949060-47949079 None:intergenic 45.0%
CATGAGAGAGGACGTGATTT+AGG + chr4_4:47949198-47949217 None:intergenic 45.0%
! GCAAAATGGGTCTGATTCGT+CGG - chr4_4:47949297-47949316 Msa0684580:exon 45.0%
!! TTGAGTCGAAGTGTTGGACT+CGG - chr4_4:47949352-47949371 Msa0684580:five_prime_UTR 45.0%
!!! TTTTGGCGGGCTTAAACATG+TGG - chr4_4:47949409-47949428 Msa0684580:five_prime_UTR 45.0%
TGGGTGCAGGAACAAAATTG+AGG - chr4_4:47948285-47948304 Msa0684580:three_prime_UTR 45.0%
TGTATGTCGCACCTCATTCT+CGG + chr4_4:47948342-47948361 None:intergenic 45.0%
GATTGCAGCCAAATTGGAAG+AGG - chr4_4:47948515-47948534 Msa0684580:three_prime_UTR 45.0%
GGAAGAGGATATGTCTCTTC+AGG - chr4_4:47948530-47948549 Msa0684580:three_prime_UTR 45.0%
AACTGCCCTATTTGAGAACG+AGG - chr4_4:47948584-47948603 Msa0684580:exon 45.0%
GCGAACCTCGTTCTCAAATA+GGG + chr4_4:47948592-47948611 None:intergenic 45.0%
AAACAACCGGAGCATATTGC+AGG - chr4_4:47948651-47948670 Msa0684580:intron 45.0%
AATGGACCTGCAATATGCTC+CGG + chr4_4:47948660-47948679 None:intergenic 45.0%
ACCCCCTTACATATCTTCAG+TGG + chr4_4:47949060-47949079 None:intergenic 45.0%
CATGAGAGAGGACGTGATTT+AGG + chr4_4:47949198-47949217 None:intergenic 45.0%
! GCAAAATGGGTCTGATTCGT+CGG - chr4_4:47949297-47949316 Msa0684580:exon 45.0%
!! TTGAGTCGAAGTGTTGGACT+CGG - chr4_4:47949352-47949371 Msa0684580:five_prime_UTR 45.0%
!!! TTTTGGCGGGCTTAAACATG+TGG - chr4_4:47949409-47949428 Msa0684580:five_prime_UTR 45.0%
!! TTATTCTAAATATTTATTAT+TGG - chr4_4:47948265-47948284 Msa0684580:three_prime_UTR 5.0%
!! TATTCTAAATATTTATTATT+GGG - chr4_4:47948266-47948285 Msa0684580:three_prime_UTR 5.0%
!! TTATTCTAAATATTTATTAT+TGG - chr4_4:47948265-47948284 Msa0684580:three_prime_UTR 5.0%
!! TATTCTAAATATTTATTATT+GGG - chr4_4:47948266-47948285 Msa0684580:three_prime_UTR 5.0%
CGCGAACCTCGTTCTCAAAT+AGG + chr4_4:47948593-47948612 None:intergenic 50.0%
TCGCGCTGCTATGTTGAAAG+AGG - chr4_4:47948608-47948627 Msa0684580:CDS 50.0%
GGGCTTAAACATGTGGCTGA+CGG - chr4_4:47949416-47949435 Msa0684580:five_prime_UTR 50.0%
CGCGAACCTCGTTCTCAAAT+AGG + chr4_4:47948593-47948612 None:intergenic 50.0%
TCGCGCTGCTATGTTGAAAG+AGG - chr4_4:47948608-47948627 Msa0684580:CDS 50.0%
GGGCTTAAACATGTGGCTGA+CGG - chr4_4:47949416-47949435 Msa0684580:five_prime_UTR 50.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 47948166 47949455 47948166 ID=Msa0684580;Name=Msa0684580
chr4_4 mRNA 47948166 47949455 47948166 ID=Msa0684580-mRNA-1;Parent=Msa0684580;Name=Msa0684580-mRNA-1;_AED=0.34;_eAED=0.34;_QI=157|1|1|1|1|1|3|437|154
chr4_4 exon 47948166 47948645 47948166 ID=Msa0684580-mRNA-1:exon:11871;Parent=Msa0684580-mRNA-1
chr4_4 exon 47948726 47949120 47948726 ID=Msa0684580-mRNA-1:exon:11870;Parent=Msa0684580-mRNA-1
chr4_4 exon 47949272 47949455 47949272 ID=Msa0684580-mRNA-1:exon:11869;Parent=Msa0684580-mRNA-1
chr4_4 five_prime_UTR 47949299 47949455 47949299 ID=Msa0684580-mRNA-1:five_prime_utr;Parent=Msa0684580-mRNA-1
chr4_4 CDS 47949272 47949298 47949272 ID=Msa0684580-mRNA-1:cds;Parent=Msa0684580-mRNA-1
chr4_4 CDS 47948726 47949120 47948726 ID=Msa0684580-mRNA-1:cds;Parent=Msa0684580-mRNA-1
chr4_4 CDS 47948603 47948645 47948603 ID=Msa0684580-mRNA-1:cds;Parent=Msa0684580-mRNA-1
chr4_4 three_prime_UTR 47948166 47948602 47948166 ID=Msa0684580-mRNA-1:three_prime_utr;Parent=Msa0684580-mRNA-1
Gene Sequence

>Msa0684580

ATGAAGTTCAACATTTCCGAGAATGAGCAAATTGATGAATTGATTGCAGCCAAATTGGAAGAGGATATGTCTCTTCAGGATTCATTGAGAAAGGTTCATGACTTGTTTATAACTGCCCTATTTGAGAACGAGGTTCGCGCTGCTATGTTGAAAGAGGAGACGGATGCAATAATTGAGAAACAACCGGAGCATATTGCAGGTCCATTATGTAAAGAGGTCAGTAAAATATACATGAAACTGAGAACAGAGGTCAGTGAATTCTTCAATATTGATGATATAATTGAGGAACAATCGGGGCGTGTTCCAGTTGCATTGACAATAGAGGTCATTAAAACGAACATGAAATTGCGAAAATTTATCGATGAATTGTACATGGATGGACAAGAAAGGTATGCTGCTTTGTATGTGGAGAAGTCTAGTCGGTTAATCAAAGACAAAACAGAAAAGCAAGACAGAAAGTCATAG
Protein sequence

>Msa0684580

MKFNISENEQIDELIAAKLEEDMSLQDSLRKVHDLFITALFENEVRAAMLKEETDAIIEKQPEHIAGPLCKEVSKIYMKLRTEVSEFFNIDDIIEEQSGRVPVALTIEVIKTNMKLRKFIDELYMDGQERYAALYVEKSSRLIKDKTEKQDRKS*