AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0551390

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0551390 MtrunA17_Chr4g0037021 94.805 77 4 0 3 79 121 197 4.81e-48 150
Msa0551390 MtrunA17_Chr8g0371501 80.519 77 15 0 3 79 121 197 1.31e-30 106
Msa0551390 MtrunA17_Chr5g0405791 79.221 77 16 0 3 79 121 197 4.88e-29 102
Msa0551390 MtrunA17_Chr6g0486041 60.759 79 28 1 3 78 121 199 2.16e-26 95.9
Msa0551390 MtrunA17_Chr3g0117931 55.844 77 33 1 3 78 123 199 4.50e-26 95.1
Msa0551390 MtrunA17_Chr2g0325101 72.414 58 16 0 3 60 121 178 1.09e-24 91.3
Msa0551390 MtrunA17_Chr4g0046221 75.676 37 9 0 24 60 10 46 1.19e-14 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0551390 AT4G35020.1 78.205 78 16 1 3 79 121 198 5.96e-32 110
Msa0551390 AT4G35020.2 78.205 78 16 1 3 79 121 198 5.96e-32 110
Msa0551390 AT4G35020.3 78.205 78 16 1 3 79 121 198 5.96e-32 110
Msa0551390 AT3G51300.1 81.818 77 14 0 3 79 121 197 1.26e-30 106
Msa0551390 AT2G17800.2 80.519 77 15 0 3 79 121 197 1.97e-30 106
Msa0551390 AT2G17800.1 80.519 77 15 0 3 79 121 197 1.97e-30 106
Msa0551390 AT4G35950.1 77.922 77 17 0 3 79 121 197 2.54e-29 103
Msa0551390 AT1G75840.1 81.034 58 11 0 3 60 121 178 5.24e-29 102
Msa0551390 AT1G20090.1 81.034 58 11 0 3 60 120 177 4.27e-28 100
Msa0551390 AT3G51290.2 81.818 77 14 0 3 79 722 798 4.55e-28 105
Msa0551390 AT5G45970.1 74.576 59 15 0 3 61 121 179 2.60e-26 95.9
Msa0551390 AT5G62880.1 71.667 60 17 0 3 62 123 182 8.06e-26 94.7
Msa0551390 AT3G48040.1 66.129 62 21 0 3 64 123 184 6.01e-25 92.4
Msa0551390 AT4G28950.1 60.000 80 26 3 3 78 121 198 1.39e-23 89.0
Msa0551390 AT2G44690.1 57.143 77 33 0 3 79 133 209 2.11e-23 88.6
Msa0551390 AT5G62880.2 64.583 48 17 0 3 50 123 170 8.15e-17 71.2
Msa0551390 AT3G48040.2 63.636 44 16 0 3 46 123 166 3.22e-15 66.6

Find 47 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAAACACTTGAAAGAAAT+TGG 0.242653 4_1:+47359301 None:intergenic
TCACATTGTAATCTAATCTT+AGG 0.297634 4_1:-47359433 Msa0551390:three_prime_UTR
GTTCTTCGTATTGTAGTTCA+TGG 0.334728 4_1:-47359731 Msa0551390:three_prime_UTR
TTAACAACCTGAATATAGTT+TGG 0.340163 4_1:-47359345 Msa0551390:three_prime_UTR
ATATAGTTTGGTTTGTTCAT+TGG 0.348235 4_1:-47359333 Msa0551390:three_prime_UTR
AGTCATAATTTCAACATGTT+TGG 0.352448 4_1:-47359543 Msa0551390:three_prime_UTR
GCTTGCAACTTATGCGGCTT+TGG 0.360502 4_1:-47359406 Msa0551390:three_prime_UTR
GAACTGCATTCAATGTAAAC+TGG 0.374474 4_1:+47359963 None:intergenic
AAGAACTGAAGATCACAAAA+TGG 0.386954 4_1:+47359748 None:intergenic
GTACAACTATTGTGTTTCTC+TGG 0.415292 4_1:-47359517 Msa0551390:three_prime_UTR
TAACTAAGCCAACTGAACTT+TGG 0.417976 4_1:+47359251 None:intergenic
ACATGACAGTAGCCATTTCC+TGG 0.419708 4_1:-47359702 Msa0551390:three_prime_UTR
GATGATGGTCAGTTCTTTCA+AGG 0.430658 4_1:-47360209 Msa0551390:CDS
GAACAAACCAAACTATATTC+AGG 0.437094 4_1:+47359338 None:intergenic
AACAAAGAGAAAGGGGCAAA+AGG 0.438650 4_1:-47359777 Msa0551390:CDS
CAAAAGAAAACAAAGAGAAA+GGG 0.440459 4_1:-47359785 Msa0551390:CDS
GCAAAAGAAAACAAAGAGAA+AGG 0.444377 4_1:-47359786 Msa0551390:CDS
TTTATAATCCAAAGTTCAGT+TGG 0.447690 4_1:-47359259 Msa0551390:three_prime_UTR
GTCATCTCCATTTGTGGACA+TGG 0.452703 4_1:-47359463 Msa0551390:three_prime_UTR
CAAATGGTCATCTCCATTTG+TGG 0.458996 4_1:-47359469 Msa0551390:three_prime_UTR
TTTGTAGATCTTCGGGATGA+TGG 0.462847 4_1:-47360224 Msa0551390:intron
AACATCAAGCTATTTGTCTC+TGG 0.464278 4_1:-47359584 Msa0551390:three_prime_UTR
CAGTTCTTTCAAGGCCATCC+TGG 0.473256 4_1:-47360200 Msa0551390:CDS
AGTACCTGTGCTGTGGTGAT+TGG 0.474297 4_1:+47360170 None:intergenic
TGAAGATCACAAAATGGAAC+AGG 0.487806 4_1:+47359754 None:intergenic
GTGGTGATTGGTGCCGCACC+AGG 0.493084 4_1:+47360182 None:intergenic
GTAAAACCCATGTCCACAAA+TGG 0.499593 4_1:+47359456 None:intergenic
GAGGAACTGAGAAAACTTAT+CGG 0.501231 4_1:-47359990 Msa0551390:CDS
TGGCTGTAGAACTACCTTGA+TGG 0.522363 4_1:+47359814 None:intergenic
CAAGCTTTAACGCGGTCAGT+TGG 0.532328 4_1:+47359614 None:intergenic
TCATCTCCATTTGTGGACAT+GGG 0.536199 4_1:-47359462 Msa0551390:three_prime_UTR
ATGCAGTTCCAAAACACAAA+AGG 0.539727 4_1:-47359949 Msa0551390:intron
ACATGGCGGTTCCTCCATCT+AGG 0.541209 4_1:+47359637 None:intergenic
CATTTGATAATCAATACAAA+AGG 0.558185 4_1:+47359486 None:intergenic
AGCTATTTGTCTCTGGCTGA+AGG 0.559006 4_1:-47359577 Msa0551390:three_prime_UTR
TATAAGATATATAATCAGAA+TGG 0.561705 4_1:+47359670 None:intergenic
TTAACGCGGTCAGTTGGACA+TGG 0.567380 4_1:+47359620 None:intergenic
AAAAGAAAACAAAGAGAAAG+GGG 0.580909 4_1:-47359784 Msa0551390:CDS
CTTTCAAGGCCATCCTGGTG+CGG 0.584915 4_1:-47360195 Msa0551390:CDS
GGAAGTGCTTGCAACTTATG+CGG 0.594099 4_1:-47359412 Msa0551390:three_prime_UTR
GGCACCAATCACCACAGCAC+AGG 0.596380 4_1:-47360174 Msa0551390:intron
TGATTGGTGCCGCACCAGGA+TGG 0.605450 4_1:+47360186 None:intergenic
CTTCGTATTGTAGTTCATGG+AGG 0.635481 4_1:-47359728 Msa0551390:three_prime_UTR
CCTTTGGATACAGAATGTGA+AGG 0.689990 4_1:-47359852 Msa0551390:intron
ACGCGGTCAGTTGGACATGG+CGG 0.694583 4_1:+47359623 None:intergenic
TTTATAAGCAAGCTTTAACG+CGG 0.695111 4_1:+47359606 None:intergenic
AATAGCAAGTACCTGTGCTG+TGG 0.726635 4_1:+47360163 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAAATATTTTTTATTCTC+TGG - chr4_1:47359546-47359565 Msa0551390:three_prime_UTR 10.0%
!! TTAAACATATATATATTTGA+TGG - chr4_1:47359895-47359914 Msa0551390:intron 10.0%
!!! ATTAAATATTTTTTATTCTC+TGG - chr4_1:47359546-47359565 Msa0551390:three_prime_UTR 10.0%
!! TTAAACATATATATATTTGA+TGG - chr4_1:47359895-47359914 Msa0551390:intron 10.0%
!! AAATACCTTTAATGAAATTT+CGG - chr4_1:47359350-47359369 Msa0551390:three_prime_UTR 15.0%
!!! CACTTTTTAAATTTATTTTC+AGG + chr4_1:47359620-47359639 None:intergenic 15.0%
!!! ATATTTTTACTCCTATATAT+AGG - chr4_1:47359865-47359884 Msa0551390:intron 15.0%
!!! TATTTTTACTCCTATATATA+GGG - chr4_1:47359866-47359885 Msa0551390:intron 15.0%
!! TAAAAGACAAACAATTAAAT+AGG + chr4_1:47360113-47360132 None:intergenic 15.0%
!! TATAAGATATATAATCAGAA+TGG + chr4_1:47360536-47360555 None:intergenic 15.0%
!! AAATACCTTTAATGAAATTT+CGG - chr4_1:47359350-47359369 Msa0551390:three_prime_UTR 15.0%
!!! CACTTTTTAAATTTATTTTC+AGG + chr4_1:47359620-47359639 None:intergenic 15.0%
!!! ATATTTTTACTCCTATATAT+AGG - chr4_1:47359865-47359884 Msa0551390:intron 15.0%
!!! TATTTTTACTCCTATATATA+GGG - chr4_1:47359866-47359885 Msa0551390:intron 15.0%
!! TAAAAGACAAACAATTAAAT+AGG + chr4_1:47360113-47360132 None:intergenic 15.0%
!! TATAAGATATATAATCAGAA+TGG + chr4_1:47360536-47360555 None:intergenic 15.0%
!!! TTTTTGACTAGTTATTTGTA+TGG - chr4_1:47359671-47359690 Msa0551390:three_prime_UTR 20.0%
!! TTAAGTTAAACCCTATATAT+AGG + chr4_1:47359879-47359898 None:intergenic 20.0%
!!! TGATTTACTATATTTTCTGA+AGG - chr4_1:47360188-47360207 Msa0551390:CDS 20.0%
!!! GATTTACTATATTTTCTGAA+GGG - chr4_1:47360189-47360208 Msa0551390:CDS 20.0%
!!! TATATATACCTTTTGTGTTT+TGG + chr4_1:47360265-47360284 None:intergenic 20.0%
!!! CATTTGATAATCAATACAAA+AGG + chr4_1:47360720-47360739 None:intergenic 20.0%
!!! CTTTTGTATTGATTATCAAA+TGG - chr4_1:47360718-47360737 Msa0551390:intron 20.0%
!! AATAAACACTTGAAAGAAAT+TGG + chr4_1:47360905-47360924 None:intergenic 20.0%
!!! TTTTTGACTAGTTATTTGTA+TGG - chr4_1:47359671-47359690 Msa0551390:three_prime_UTR 20.0%
!! TTAAGTTAAACCCTATATAT+AGG + chr4_1:47359879-47359898 None:intergenic 20.0%
!!! TGATTTACTATATTTTCTGA+AGG - chr4_1:47360188-47360207 Msa0551390:CDS 20.0%
!!! GATTTACTATATTTTCTGAA+GGG - chr4_1:47360189-47360208 Msa0551390:CDS 20.0%
!!! TATATATACCTTTTGTGTTT+TGG + chr4_1:47360265-47360284 None:intergenic 20.0%
!!! CATTTGATAATCAATACAAA+AGG + chr4_1:47360720-47360739 None:intergenic 20.0%
!!! CTTTTGTATTGATTATCAAA+TGG - chr4_1:47360718-47360737 Msa0551390:intron 20.0%
!! AATAAACACTTGAAAGAAAT+TGG + chr4_1:47360905-47360924 None:intergenic 20.0%
! AAATATTTAATTGAGCGCAA+TGG + chr4_1:47359537-47359556 None:intergenic 25.0%
!! ATCTATGTTTTGTAGATCTT+CGG - chr4_1:47359971-47359990 Msa0551390:CDS 25.0%
! GCATAGTTATTAGCAAATAA+AGG + chr4_1:47360137-47360156 None:intergenic 25.0%
!! ATTTGAATCTTTGAAAGTTC+TGG - chr4_1:47360294-47360313 Msa0551390:intron 25.0%
! CAAAAGAAAACAAAGAGAAA+GGG - chr4_1:47360418-47360437 Msa0551390:intron 25.0%
! AAAAGAAAACAAAGAGAAAG+GGG - chr4_1:47360419-47360438 Msa0551390:intron 25.0%
! AGTCATAATTTCAACATGTT+TGG - chr4_1:47360660-47360679 Msa0551390:intron 25.0%
! TCACATTGTAATCTAATCTT+AGG - chr4_1:47360770-47360789 Msa0551390:intron 25.0%
! TTAACAACCTGAATATAGTT+TGG - chr4_1:47360858-47360877 Msa0551390:intron 25.0%
!!! ATATAGTTTGGTTTGTTCAT+TGG - chr4_1:47360870-47360889 Msa0551390:intron 25.0%
! TTTATAATCCAAAGTTCAGT+TGG - chr4_1:47360944-47360963 Msa0551390:intron 25.0%
! AAATATTTAATTGAGCGCAA+TGG + chr4_1:47359537-47359556 None:intergenic 25.0%
!! ATCTATGTTTTGTAGATCTT+CGG - chr4_1:47359971-47359990 Msa0551390:CDS 25.0%
! GCATAGTTATTAGCAAATAA+AGG + chr4_1:47360137-47360156 None:intergenic 25.0%
!! ATTTGAATCTTTGAAAGTTC+TGG - chr4_1:47360294-47360313 Msa0551390:intron 25.0%
! CAAAAGAAAACAAAGAGAAA+GGG - chr4_1:47360418-47360437 Msa0551390:intron 25.0%
! AAAAGAAAACAAAGAGAAAG+GGG - chr4_1:47360419-47360438 Msa0551390:intron 25.0%
! AGTCATAATTTCAACATGTT+TGG - chr4_1:47360660-47360679 Msa0551390:intron 25.0%
! TCACATTGTAATCTAATCTT+AGG - chr4_1:47360770-47360789 Msa0551390:intron 25.0%
! TTAACAACCTGAATATAGTT+TGG - chr4_1:47360858-47360877 Msa0551390:intron 25.0%
!!! ATATAGTTTGGTTTGTTCAT+TGG - chr4_1:47360870-47360889 Msa0551390:intron 25.0%
! TTTATAATCCAAAGTTCAGT+TGG - chr4_1:47360944-47360963 Msa0551390:intron 25.0%
AACTGCCGAAATTTCATTAA+AGG + chr4_1:47359358-47359377 None:intergenic 30.0%
GGAACAAATTATAAAGCTTC+CGG + chr4_1:47359433-47359452 None:intergenic 30.0%
! TTGACTAGTTATTTGTATGG+TGG - chr4_1:47359674-47359693 Msa0551390:three_prime_UTR 30.0%
!!! AGTTTTCACAGTTGCTTTTT+TGG - chr4_1:47359841-47359860 Msa0551390:CDS 30.0%
!! TTGTTTGCTTGGCTATTTTA+TGG - chr4_1:47359941-47359960 Msa0551390:intron 30.0%
! TCTATGTTTTGTAGATCTTC+GGG - chr4_1:47359972-47359991 Msa0551390:CDS 30.0%
!! TTGAAAGTTCTGGAGTTTAA+AGG - chr4_1:47360304-47360323 Msa0551390:intron 30.0%
!! TGAAAGTTCTGGAGTTTAAA+GGG - chr4_1:47360305-47360324 Msa0551390:intron 30.0%
GGTTAACATAGATAAGGTTA+CGG + chr4_1:47360333-47360352 None:intergenic 30.0%
ACCTTATCTATGTTAACCTT+TGG - chr4_1:47360335-47360354 Msa0551390:intron 30.0%
TCCAAAGGTTAACATAGATA+AGG + chr4_1:47360339-47360358 None:intergenic 30.0%
!! TTTGTTTTCTTTTGCATTGG+TGG + chr4_1:47360412-47360431 None:intergenic 30.0%
!!! CTCTTTGTTTTCTTTTGCAT+TGG + chr4_1:47360415-47360434 None:intergenic 30.0%
GCAAAAGAAAACAAAGAGAA+AGG - chr4_1:47360417-47360436 Msa0551390:intron 30.0%
AAGAACTGAAGATCACAAAA+TGG + chr4_1:47360458-47360477 None:intergenic 30.0%
! TATACTATTTTCGACCTAGA+TGG - chr4_1:47360552-47360571 Msa0551390:intron 30.0%
! TTTATAAGCAAGCTTTAACG+CGG + chr4_1:47360600-47360619 None:intergenic 30.0%
GAACAAACCAAACTATATTC+AGG + chr4_1:47360868-47360887 None:intergenic 30.0%
AACTGCCGAAATTTCATTAA+AGG + chr4_1:47359358-47359377 None:intergenic 30.0%
GGAACAAATTATAAAGCTTC+CGG + chr4_1:47359433-47359452 None:intergenic 30.0%
! TTGACTAGTTATTTGTATGG+TGG - chr4_1:47359674-47359693 Msa0551390:three_prime_UTR 30.0%
!!! AGTTTTCACAGTTGCTTTTT+TGG - chr4_1:47359841-47359860 Msa0551390:CDS 30.0%
!! TTGTTTGCTTGGCTATTTTA+TGG - chr4_1:47359941-47359960 Msa0551390:intron 30.0%
! TCTATGTTTTGTAGATCTTC+GGG - chr4_1:47359972-47359991 Msa0551390:CDS 30.0%
!! TTGAAAGTTCTGGAGTTTAA+AGG - chr4_1:47360304-47360323 Msa0551390:intron 30.0%
!! TGAAAGTTCTGGAGTTTAAA+GGG - chr4_1:47360305-47360324 Msa0551390:intron 30.0%
GGTTAACATAGATAAGGTTA+CGG + chr4_1:47360333-47360352 None:intergenic 30.0%
ACCTTATCTATGTTAACCTT+TGG - chr4_1:47360335-47360354 Msa0551390:intron 30.0%
TCCAAAGGTTAACATAGATA+AGG + chr4_1:47360339-47360358 None:intergenic 30.0%
!! TTTGTTTTCTTTTGCATTGG+TGG + chr4_1:47360412-47360431 None:intergenic 30.0%
!!! CTCTTTGTTTTCTTTTGCAT+TGG + chr4_1:47360415-47360434 None:intergenic 30.0%
GCAAAAGAAAACAAAGAGAA+AGG - chr4_1:47360417-47360436 Msa0551390:intron 30.0%
AAGAACTGAAGATCACAAAA+TGG + chr4_1:47360458-47360477 None:intergenic 30.0%
! TATACTATTTTCGACCTAGA+TGG - chr4_1:47360552-47360571 Msa0551390:intron 30.0%
! TTTATAAGCAAGCTTTAACG+CGG + chr4_1:47360600-47360619 None:intergenic 30.0%
GAACAAACCAAACTATATTC+AGG + chr4_1:47360868-47360887 None:intergenic 30.0%
TATAAAGCTTCCGGTAATCT+CGG + chr4_1:47359424-47359443 None:intergenic 35.0%
GTTCCTGAACATTGTAAACA+TGG - chr4_1:47359448-47359467 Msa0551390:three_prime_UTR 35.0%
TAGCCATGTTTACAATGTTC+AGG + chr4_1:47359454-47359473 None:intergenic 35.0%
TGGCTAATTCTATCCAGAAA+CGG - chr4_1:47359468-47359487 Msa0551390:three_prime_UTR 35.0%
TTAAAGACTCCCGTGAATTT+CGG + chr4_1:47359585-47359604 None:intergenic 35.0%
ACCGCTTAATTACAAGTTCA+CGG - chr4_1:47359700-47359719 Msa0551390:three_prime_UTR 35.0%
ATGAAGTCAAGGACTTATTC+AGG + chr4_1:47359748-47359767 None:intergenic 35.0%
!! CTTGTCATGATTTGTTTGCT+TGG - chr4_1:47359930-47359949 Msa0551390:intron 35.0%
GCAACTAACAGTATATATGC+AGG + chr4_1:47360077-47360096 None:intergenic 35.0%
!! ACTATATTTTCTGAAGGGTG+AGG - chr4_1:47360194-47360213 Msa0551390:CDS 35.0%
GAGGAACTGAGAAAACTTAT+CGG - chr4_1:47360213-47360232 Msa0551390:CDS 35.0%
GAACTGCATTCAATGTAAAC+TGG + chr4_1:47360243-47360262 None:intergenic 35.0%
ATGCAGTTCCAAAACACAAA+AGG - chr4_1:47360254-47360273 Msa0551390:intron 35.0%
TGAAGATCACAAAATGGAAC+AGG + chr4_1:47360452-47360471 None:intergenic 35.0%
GTTCTTCGTATTGTAGTTCA+TGG - chr4_1:47360472-47360491 Msa0551390:intron 35.0%
AACATCAAGCTATTTGTCTC+TGG - chr4_1:47360619-47360638 Msa0551390:intron 35.0%
GTACAACTATTGTGTTTCTC+TGG - chr4_1:47360686-47360705 Msa0551390:intron 35.0%
TATAAAGCTTCCGGTAATCT+CGG + chr4_1:47359424-47359443 None:intergenic 35.0%
GTTCCTGAACATTGTAAACA+TGG - chr4_1:47359448-47359467 Msa0551390:three_prime_UTR 35.0%
TAGCCATGTTTACAATGTTC+AGG + chr4_1:47359454-47359473 None:intergenic 35.0%
TGGCTAATTCTATCCAGAAA+CGG - chr4_1:47359468-47359487 Msa0551390:three_prime_UTR 35.0%
TTAAAGACTCCCGTGAATTT+CGG + chr4_1:47359585-47359604 None:intergenic 35.0%
ACCGCTTAATTACAAGTTCA+CGG - chr4_1:47359700-47359719 Msa0551390:three_prime_UTR 35.0%
ATGAAGTCAAGGACTTATTC+AGG + chr4_1:47359748-47359767 None:intergenic 35.0%
!! CTTGTCATGATTTGTTTGCT+TGG - chr4_1:47359930-47359949 Msa0551390:intron 35.0%
GCAACTAACAGTATATATGC+AGG + chr4_1:47360077-47360096 None:intergenic 35.0%
!! ACTATATTTTCTGAAGGGTG+AGG - chr4_1:47360194-47360213 Msa0551390:CDS 35.0%
GAGGAACTGAGAAAACTTAT+CGG - chr4_1:47360213-47360232 Msa0551390:CDS 35.0%
GAACTGCATTCAATGTAAAC+TGG + chr4_1:47360243-47360262 None:intergenic 35.0%
ATGCAGTTCCAAAACACAAA+AGG - chr4_1:47360254-47360273 Msa0551390:intron 35.0%
TGAAGATCACAAAATGGAAC+AGG + chr4_1:47360452-47360471 None:intergenic 35.0%
GTTCTTCGTATTGTAGTTCA+TGG - chr4_1:47360472-47360491 Msa0551390:intron 35.0%
AACATCAAGCTATTTGTCTC+TGG - chr4_1:47360619-47360638 Msa0551390:intron 35.0%
GTACAACTATTGTGTTTCTC+TGG - chr4_1:47360686-47360705 Msa0551390:intron 35.0%
TTCATTCTGTGATACGCACT+TGG + chr4_1:47359264-47359283 None:intergenic 40.0%
! ATGTAGCCTCGAAGCTTTTT+AGG - chr4_1:47359393-47359412 Msa0551390:three_prime_UTR 40.0%
GGCTAATTCTATCCAGAAAC+GGG - chr4_1:47359469-47359488 Msa0551390:three_prime_UTR 40.0%
CACAACAAACCCGAAATTCA+CGG - chr4_1:47359572-47359591 Msa0551390:three_prime_UTR 40.0%
ACAACAAACCCGAAATTCAC+GGG - chr4_1:47359573-47359592 Msa0551390:three_prime_UTR 40.0%
TAAAGACTCCCGTGAATTTC+GGG + chr4_1:47359584-47359603 None:intergenic 40.0%
ACGGGAGTCTTTAAGTCATA+AGG - chr4_1:47359591-47359610 Msa0551390:three_prime_UTR 40.0%
CCCGTGAACTTGTAATTAAG+CGG + chr4_1:47359704-47359723 None:intergenic 40.0%
CCGCTTAATTACAAGTTCAC+GGG - chr4_1:47359701-47359720 Msa0551390:three_prime_UTR 40.0%
ACGGGAGTCTTTAAGTCATA+AGG - chr4_1:47359591-47359610 Msa0551390:three_prime_UTR 40.0%
! TCTGACTAGTCATGAAGTCA+AGG + chr4_1:47359759-47359778 None:intergenic 40.0%
TTTGTAGATCTTCGGGATGA+TGG - chr4_1:47359979-47359998 Msa0551390:CDS 40.0%
! GATGATGGTCAGTTCTTTCA+AGG - chr4_1:47359994-47360013 Msa0551390:CDS 40.0%
CCTTCACATTCTGTATCCAA+AGG + chr4_1:47360354-47360373 None:intergenic 40.0%
CCTTTGGATACAGAATGTGA+AGG - chr4_1:47360351-47360370 Msa0551390:intron 40.0%
!!! TGTGAAGGCCGTTTTTGATT+CGG - chr4_1:47360366-47360385 Msa0551390:intron 40.0%
! CTTGATGGCCGAATCAAAAA+CGG + chr4_1:47360377-47360396 None:intergenic 40.0%
AACAAAGAGAAAGGGGCAAA+AGG - chr4_1:47360426-47360445 Msa0551390:intron 40.0%
CTTCGTATTGTAGTTCATGG+AGG - chr4_1:47360475-47360494 Msa0551390:intron 40.0%
TGGCAAAAACTACCAGGAAA+TGG + chr4_1:47360516-47360535 None:intergenic 40.0%
TCAGAATGGCAAAAACTACC+AGG + chr4_1:47360522-47360541 None:intergenic 40.0%
! ACTATTTTCGACCTAGATGG+AGG - chr4_1:47360555-47360574 Msa0551390:intron 40.0%
CAAATGGTCATCTCCATTTG+TGG - chr4_1:47360734-47360753 Msa0551390:intron 40.0%
TCATCTCCATTTGTGGACAT+GGG - chr4_1:47360741-47360760 Msa0551390:intron 40.0%
GTAAAACCCATGTCCACAAA+TGG + chr4_1:47360750-47360769 None:intergenic 40.0%
TTCATTCTGTGATACGCACT+TGG + chr4_1:47359264-47359283 None:intergenic 40.0%
! ATGTAGCCTCGAAGCTTTTT+AGG - chr4_1:47359393-47359412 Msa0551390:three_prime_UTR 40.0%
GGCTAATTCTATCCAGAAAC+GGG - chr4_1:47359469-47359488 Msa0551390:three_prime_UTR 40.0%
CACAACAAACCCGAAATTCA+CGG - chr4_1:47359572-47359591 Msa0551390:three_prime_UTR 40.0%
ACAACAAACCCGAAATTCAC+GGG - chr4_1:47359573-47359592 Msa0551390:three_prime_UTR 40.0%
TAAAGACTCCCGTGAATTTC+GGG + chr4_1:47359584-47359603 None:intergenic 40.0%
ACGGGAGTCTTTAAGTCATA+AGG - chr4_1:47359591-47359610 Msa0551390:three_prime_UTR 40.0%
CCCGTGAACTTGTAATTAAG+CGG + chr4_1:47359704-47359723 None:intergenic 40.0%
CCGCTTAATTACAAGTTCAC+GGG - chr4_1:47359701-47359720 Msa0551390:three_prime_UTR 40.0%
ACGGGAGTCTTTAAGTCATA+AGG - chr4_1:47359591-47359610 Msa0551390:three_prime_UTR 40.0%
! TCTGACTAGTCATGAAGTCA+AGG + chr4_1:47359759-47359778 None:intergenic 40.0%
TTTGTAGATCTTCGGGATGA+TGG - chr4_1:47359979-47359998 Msa0551390:CDS 40.0%
! GATGATGGTCAGTTCTTTCA+AGG - chr4_1:47359994-47360013 Msa0551390:CDS 40.0%
CCTTCACATTCTGTATCCAA+AGG + chr4_1:47360354-47360373 None:intergenic 40.0%
CCTTTGGATACAGAATGTGA+AGG - chr4_1:47360351-47360370 Msa0551390:intron 40.0%
!!! TGTGAAGGCCGTTTTTGATT+CGG - chr4_1:47360366-47360385 Msa0551390:intron 40.0%
! CTTGATGGCCGAATCAAAAA+CGG + chr4_1:47360377-47360396 None:intergenic 40.0%
AACAAAGAGAAAGGGGCAAA+AGG - chr4_1:47360426-47360445 Msa0551390:intron 40.0%
CTTCGTATTGTAGTTCATGG+AGG - chr4_1:47360475-47360494 Msa0551390:intron 40.0%
TGGCAAAAACTACCAGGAAA+TGG + chr4_1:47360516-47360535 None:intergenic 40.0%
TCAGAATGGCAAAAACTACC+AGG + chr4_1:47360522-47360541 None:intergenic 40.0%
! ACTATTTTCGACCTAGATGG+AGG - chr4_1:47360555-47360574 Msa0551390:intron 40.0%
CAAATGGTCATCTCCATTTG+TGG - chr4_1:47360734-47360753 Msa0551390:intron 40.0%
TCATCTCCATTTGTGGACAT+GGG - chr4_1:47360741-47360760 Msa0551390:intron 40.0%
GTAAAACCCATGTCCACAAA+TGG + chr4_1:47360750-47360769 None:intergenic 40.0%
AATTTCGGCAGTTACACCTG+AGG - chr4_1:47359365-47359384 Msa0551390:three_prime_UTR 45.0%
!! TAGCCTCGAAGCTTTTTAGG+AGG - chr4_1:47359396-47359415 Msa0551390:three_prime_UTR 45.0%
GTTCCTCCTAAAAAGCTTCG+AGG + chr4_1:47359402-47359421 None:intergenic 45.0%
TTAGGAGGAACCGAGATTAC+CGG - chr4_1:47359411-47359430 Msa0551390:three_prime_UTR 45.0%
TTTCACAAGAGCCTCGACTT+CGG + chr4_1:47359510-47359529 None:intergenic 45.0%
!! CGTGAATTTCGGGTTTGTTG+TGG + chr4_1:47359574-47359593 None:intergenic 45.0%
AATAGCAAGTACCTGTGCTG+TGG + chr4_1:47360043-47360062 None:intergenic 45.0%
!!! CGTTTTTGATTCGGCCATCA+AGG - chr4_1:47360375-47360394 Msa0551390:intron 45.0%
TGGCTGTAGAACTACCTTGA+TGG + chr4_1:47360392-47360411 None:intergenic 45.0%
ACATGACAGTAGCCATTTCC+TGG - chr4_1:47360501-47360520 Msa0551390:intron 45.0%
AGCTATTTGTCTCTGGCTGA+AGG - chr4_1:47360626-47360645 Msa0551390:intron 45.0%
GTCATCTCCATTTGTGGACA+TGG - chr4_1:47360740-47360759 Msa0551390:intron 45.0%
!! GGAAGTGCTTGCAACTTATG+CGG - chr4_1:47360791-47360810 Msa0551390:intron 45.0%
AATTTCGGCAGTTACACCTG+AGG - chr4_1:47359365-47359384 Msa0551390:three_prime_UTR 45.0%
!! TAGCCTCGAAGCTTTTTAGG+AGG - chr4_1:47359396-47359415 Msa0551390:three_prime_UTR 45.0%
GTTCCTCCTAAAAAGCTTCG+AGG + chr4_1:47359402-47359421 None:intergenic 45.0%
TTAGGAGGAACCGAGATTAC+CGG - chr4_1:47359411-47359430 Msa0551390:three_prime_UTR 45.0%
TTTCACAAGAGCCTCGACTT+CGG + chr4_1:47359510-47359529 None:intergenic 45.0%
!! CGTGAATTTCGGGTTTGTTG+TGG + chr4_1:47359574-47359593 None:intergenic 45.0%
AATAGCAAGTACCTGTGCTG+TGG + chr4_1:47360043-47360062 None:intergenic 45.0%
!!! CGTTTTTGATTCGGCCATCA+AGG - chr4_1:47360375-47360394 Msa0551390:intron 45.0%
TGGCTGTAGAACTACCTTGA+TGG + chr4_1:47360392-47360411 None:intergenic 45.0%
ACATGACAGTAGCCATTTCC+TGG - chr4_1:47360501-47360520 Msa0551390:intron 45.0%
AGCTATTTGTCTCTGGCTGA+AGG - chr4_1:47360626-47360645 Msa0551390:intron 45.0%
GTCATCTCCATTTGTGGACA+TGG - chr4_1:47360740-47360759 Msa0551390:intron 45.0%
!! GGAAGTGCTTGCAACTTATG+CGG - chr4_1:47360791-47360810 Msa0551390:intron 45.0%
CGAGGCTACATACAATCCTC+AGG + chr4_1:47359384-47359403 None:intergenic 50.0%
CTATGCACTAGTCCCGTTTC+TGG + chr4_1:47359484-47359503 None:intergenic 50.0%
CAGTTCTTTCAAGGCCATCC+TGG - chr4_1:47360003-47360022 Msa0551390:intron 50.0%
AGTACCTGTGCTGTGGTGAT+TGG + chr4_1:47360036-47360055 None:intergenic 50.0%
TTAACGCGGTCAGTTGGACA+TGG + chr4_1:47360586-47360605 None:intergenic 50.0%
! CAAGCTTTAACGCGGTCAGT+TGG + chr4_1:47360592-47360611 None:intergenic 50.0%
GCTTGCAACTTATGCGGCTT+TGG - chr4_1:47360797-47360816 Msa0551390:intron 50.0%
CGAGGCTACATACAATCCTC+AGG + chr4_1:47359384-47359403 None:intergenic 50.0%
CTATGCACTAGTCCCGTTTC+TGG + chr4_1:47359484-47359503 None:intergenic 50.0%
CAGTTCTTTCAAGGCCATCC+TGG - chr4_1:47360003-47360022 Msa0551390:intron 50.0%
AGTACCTGTGCTGTGGTGAT+TGG + chr4_1:47360036-47360055 None:intergenic 50.0%
TTAACGCGGTCAGTTGGACA+TGG + chr4_1:47360586-47360605 None:intergenic 50.0%
! CAAGCTTTAACGCGGTCAGT+TGG + chr4_1:47360592-47360611 None:intergenic 50.0%
GCTTGCAACTTATGCGGCTT+TGG - chr4_1:47360797-47360816 Msa0551390:intron 50.0%
GTGCATAGAAGCCGAAGTCG+AGG - chr4_1:47359496-47359515 Msa0551390:three_prime_UTR 55.0%
CTTTCAAGGCCATCCTGGTG+CGG - chr4_1:47360008-47360027 Msa0551390:intron 55.0%
ACATGGCGGTTCCTCCATCT+AGG + chr4_1:47360569-47360588 None:intergenic 55.0%
GTGCATAGAAGCCGAAGTCG+AGG - chr4_1:47359496-47359515 Msa0551390:three_prime_UTR 55.0%
CTTTCAAGGCCATCCTGGTG+CGG - chr4_1:47360008-47360027 Msa0551390:intron 55.0%
ACATGGCGGTTCCTCCATCT+AGG + chr4_1:47360569-47360588 None:intergenic 55.0%
!! TGATTGGTGCCGCACCAGGA+TGG + chr4_1:47360020-47360039 None:intergenic 60.0%
GGCACCAATCACCACAGCAC+AGG - chr4_1:47360029-47360048 Msa0551390:intron 60.0%
ACGCGGTCAGTTGGACATGG+CGG + chr4_1:47360583-47360602 None:intergenic 60.0%
!! TGATTGGTGCCGCACCAGGA+TGG + chr4_1:47360020-47360039 None:intergenic 60.0%
GGCACCAATCACCACAGCAC+AGG - chr4_1:47360029-47360048 Msa0551390:intron 60.0%
ACGCGGTCAGTTGGACATGG+CGG + chr4_1:47360583-47360602 None:intergenic 60.0%
!! GTGGTGATTGGTGCCGCACC+AGG + chr4_1:47360024-47360043 None:intergenic 65.0%
!! GTGGTGATTGGTGCCGCACC+AGG + chr4_1:47360024-47360043 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 47359258 47360967 47359258 ID=Msa0551390;Name=Msa0551390
chr4_1 mRNA 47359258 47360967 47359258 ID=Msa0551390-mRNA-1;Parent=Msa0551390;Name=Msa0551390-mRNA-1;_AED=0.45;_eAED=0.45;_QI=0|0.66|0.75|0.75|1|1|4|502|79
chr4_1 exon 47359258 47359861 47359258 ID=Msa0551390-mRNA-1:exon:12855;Parent=Msa0551390-mRNA-1
chr4_1 exon 47359950 47360015 47359950 ID=Msa0551390-mRNA-1:exon:12854;Parent=Msa0551390-mRNA-1
chr4_1 exon 47360175 47360239 47360175 ID=Msa0551390-mRNA-1:exon:12853;Parent=Msa0551390-mRNA-1
chr4_1 exon 47360961 47360967 47360961 ID=Msa0551390-mRNA-1:exon:12852;Parent=Msa0551390-mRNA-1
chr4_1 CDS 47360961 47360967 47360961 ID=Msa0551390-mRNA-1:cds;Parent=Msa0551390-mRNA-1
chr4_1 CDS 47360175 47360239 47360175 ID=Msa0551390-mRNA-1:cds;Parent=Msa0551390-mRNA-1
chr4_1 CDS 47359950 47360015 47359950 ID=Msa0551390-mRNA-1:cds;Parent=Msa0551390-mRNA-1
chr4_1 CDS 47359760 47359861 47359760 ID=Msa0551390-mRNA-1:cds;Parent=Msa0551390-mRNA-1
chr4_1 three_prime_UTR 47359258 47359759 47359258 ID=Msa0551390-mRNA-1:three_prime_utr;Parent=Msa0551390-mRNA-1
Gene Sequence

>Msa0551390

ATGCCAAATCTTCGGGATGATGGTCAGTTCTTTCAAGGCCATCCTGGTGCGGCACCAATCACCACAGCACAGGGTGAGGAACTGAGAAAACTTATCGGTGCTCCAGTTTACATTGAATGCAGTTCCAAAACACAAAAGAATGTGAAGGCCGTTTTTGATTCGGCCATCAAGGTAGTTCTACAGCCACCAATGCAAAAGAAAACAAAGAGAAAGGGGCAAAAGGCCTGTTCCATTTTGTGA
Protein sequence

>Msa0551390

MPNLRDDGQFFQGHPGAAPITTAQGEELRKLIGAPVYIECSSKTQKNVKAVFDSAIKVVLQPPMQKKTKRKGQKACSIL*