AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0552960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0552960 MtrunA17_Chr4g0038511 99.291 282 2 0 1 282 1 282 0.0 575
Msa0552960 MtrunA17_Chr1g0161461 53.957 278 110 5 6 272 77 347 3.78e-92 276
Msa0552960 MtrunA17_Chr4g0042041 44.361 133 57 3 6 137 76 192 7.73e-25 99.4
Msa0552960 MtrunA17_Chr2g0319301 52.083 96 45 1 9 103 72 167 2.87e-23 95.1
Msa0552960 MtrunA17_Chr4g0060591 45.000 100 55 0 7 106 29 128 3.85e-21 89.4
Msa0552960 MtrunA17_Chr1g0167821 40.000 140 71 4 11 144 95 227 1.35e-18 82.8
Msa0552960 MtrunA17_Chr2g0323031 45.263 95 51 1 11 104 110 204 2.18e-18 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0552960 AT3G19860.1 62.753 247 78 5 6 246 5 243 7.45e-96 284
Msa0552960 AT3G19860.3 62.753 247 78 5 6 246 36 274 1.74e-95 284
Msa0552960 AT3G19860.2 62.348 247 79 5 6 246 58 296 4.78e-95 283
Msa0552960 AT4G36060.2 43.636 275 105 7 9 274 29 262 6.93e-54 176
Msa0552960 AT4G36060.1 43.636 275 105 7 9 274 47 280 2.32e-53 175
Msa0552960 AT4G36060.4 44.255 235 89 5 9 235 29 229 8.97e-52 171
Msa0552960 AT4G36060.3 44.255 235 89 5 9 235 47 247 4.05e-51 169
Msa0552960 AT5G54680.1 37.975 158 70 4 11 167 76 206 8.65e-22 91.7
Msa0552960 AT4G14410.2 40.152 132 64 4 11 132 129 255 1.96e-18 83.2
Msa0552960 AT4G14410.3 40.152 132 64 4 11 132 129 255 1.96e-18 83.2
Msa0552960 AT4G14410.1 40.152 132 64 4 11 132 135 261 2.90e-18 82.8
Msa0552960 AT3G23210.2 35.606 132 77 2 11 134 138 269 6.53e-18 82.0
Msa0552960 AT1G51070.1 44.681 94 51 1 11 103 71 164 7.06e-18 80.9
Msa0552960 AT1G51070.2 44.681 94 51 1 11 103 137 230 1.57e-17 81.3
Msa0552960 AT3G23210.1 35.606 132 77 2 11 134 167 298 1.73e-17 81.3
Msa0552960 AT3G47640.1 37.113 97 61 0 7 103 28 124 8.63e-16 75.5
Msa0552960 AT3G47640.3 37.113 97 61 0 7 103 28 124 8.63e-16 75.5
Msa0552960 AT3G47640.2 37.113 97 61 0 7 103 28 124 8.63e-16 75.5

Find 100 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAGGAGAGTTTGTGATTA+AGG 0.196287 4_1:-49756135 None:intergenic
AATGATAAAGCGACCATTCT+AGG 0.234923 4_1:+49754898 Msa0552960:CDS
AATTGTATTCGGAGCTAAAA+AGG 0.247255 4_1:-49755360 None:intergenic
TATTTCATCTAGTCTTTGTT+TGG 0.256862 4_1:+49756169 Msa0552960:three_prime_UTR
CGAACTACCTTCTGTGAAAC+TGG 0.280327 4_1:-49755709 None:intergenic
TAATCACAAACTCTCCTAAA+AGG 0.301913 4_1:+49756138 Msa0552960:three_prime_UTR
TCCTTAGTTGCAGCTGATAC+TGG 0.331417 4_1:-49755169 None:intergenic
TTCAATTTGTCACAAGGAAT+AGG 0.340147 4_1:-49756053 None:intergenic
TGTTGTTCAACAATTGTATT+CGG 0.342434 4_1:-49755371 None:intergenic
TTCTTCATCTCACAGTGTTC+AGG 0.343603 4_1:+49755743 Msa0552960:CDS
GGTAGCCGGTCCCACGTATC+AGG 0.344490 4_1:+49755428 Msa0552960:CDS
ACCAGTATCAGCTGCAACTA+AGG 0.363603 4_1:+49755168 Msa0552960:CDS
TAGTGGAAGAGTTGTCGTTT+TGG 0.365267 4_1:-49753788 None:intergenic
TAGCAGGTGTAATTTCCATT+GGG 0.369888 4_1:-49754519 None:intergenic
AACAAGAACAAGAATAAGAT+TGG 0.370287 4_1:+49753867 Msa0552960:five_prime_UTR
TTAGGAGAGTTTGTGATTAA+GGG 0.370502 4_1:-49756134 None:intergenic
GATACGTGGGACCGGCTACC+TGG 0.378928 4_1:-49755425 None:intergenic
ACATATGTTGAACAAGGGTT+AGG 0.393166 4_1:-49755335 None:intergenic
TCTGAAATGCAGACCCTGAT+AGG 0.394717 4_1:+49754869 Msa0552960:intron
AGGATGTTGATTAGCATAGT+AGG 0.395188 4_1:-49755303 None:intergenic
TCTTTGAGCCTTGCTCTCCC+TGG 0.396650 4_1:-49755477 None:intergenic
GCAGGTACCGGCATTGGAAC+TGG 0.401184 4_1:-49755254 None:intergenic
TCCTGGGTCTTCCGCAGATC+AGG 0.409087 4_1:+49755544 Msa0552960:CDS
CTAGCAGGTGTAATTTCCAT+TGG 0.415608 4_1:-49754520 None:intergenic
GACCTTGAGTTGAAGACTCC+TGG 0.425984 4_1:+49755527 Msa0552960:CDS
GTTTACTAACTTGAGAAGTA+AGG 0.433635 4_1:-49754945 None:intergenic
TTCTTTGAGTTGTGAGGGAA+AGG 0.439952 4_1:+49756004 Msa0552960:three_prime_UTR
TCTGTTCTCTCTTCTTAACT+AGG 0.442611 4_1:+49755941 Msa0552960:three_prime_UTR
GTTCAATTTCCAGTTGTCTC+AGG 0.452373 4_1:+49755085 Msa0552960:intron
CGCCTCTTCATCCAGGTAGC+CGG 0.455426 4_1:+49755414 Msa0552960:CDS
ATAACGGAATGATCCATTGC+AGG 0.461908 4_1:-49755206 None:intergenic
AAATGATCTCAGAGAAGAAA+AGG 0.463510 4_1:+49755112 Msa0552960:CDS
GGCTACCTGGATGAAGAGGC+GGG 0.476225 4_1:-49755412 None:intergenic
TTCACAGAAGGTAGTTCGTT+AGG 0.482876 4_1:+49755714 Msa0552960:CDS
GAACCAGCAGGTACCGGCAT+TGG 0.486966 4_1:-49755260 None:intergenic
AGGCTGACCGAGAAAAGTTA+AGG 0.489877 4_1:+49754554 Msa0552960:CDS
GTTCCAATGCCGGTACCTGC+TGG 0.492349 4_1:+49755257 Msa0552960:CDS
GGATGTTGATTAGCATAGTA+GGG 0.493252 4_1:-49755302 None:intergenic
GGTGAAAAGTGAAAGGATAG+TGG 0.493351 4_1:-49753805 None:intergenic
CATATGTTGAACAAGGGTTA+GGG 0.508234 4_1:-49755334 None:intergenic
ACCGGCATTGGAACTGGATA+CGG 0.508537 4_1:-49755248 None:intergenic
ACCTTGAGTTGAAGACTCCT+GGG 0.508601 4_1:+49755528 Msa0552960:CDS
ACAAGAACAAGAATAAGATT+GGG 0.509441 4_1:+49753868 Msa0552960:five_prime_UTR
AGCTCATCAAGTAGTGTAGT+GGG 0.509577 4_1:+49755768 Msa0552960:CDS
GAAAATGTTGAACGTACGTC+TGG 0.514766 4_1:+49756201 Msa0552960:three_prime_UTR
TCCGTATCCAGTTCCAATGC+CGG 0.524495 4_1:+49755247 Msa0552960:CDS
AGACCAGTGTGGCTAAATGT+AGG 0.525327 4_1:+49756102 Msa0552960:three_prime_UTR
GAATGATCCATTGCAGGCCA+TGG 0.527462 4_1:-49755200 None:intergenic
ACCCTCACAGTAGCTTCAAA+AGG 0.527695 4_1:-49755844 None:intergenic
TGACAAATTGAAATTGCAGA+TGG 0.531031 4_1:+49756064 Msa0552960:three_prime_UTR
CAAATCATCCAGGGAGAGCA+AGG 0.531527 4_1:+49755469 Msa0552960:CDS
GAAAGGCTAGTGAATGAGAA+TGG 0.532624 4_1:+49755796 Msa0552960:exon
TGCAATTTCAATTTGTCACA+AGG 0.534577 4_1:-49756059 None:intergenic
ATCTAGTAAGTCGTCGAGGA+AGG 0.538754 4_1:+49755683 Msa0552960:CDS
TTGAACTGTATCTCCTAGAA+TGG 0.541064 4_1:-49754911 None:intergenic
AATGGATCATTCCGTTATGA+TGG 0.543467 4_1:+49755211 Msa0552960:CDS
GGAATTGAACCAGCAGGTAC+CGG 0.545624 4_1:-49755266 None:intergenic
ACCTGCTAGAAAGACACAAA+AGG 0.554651 4_1:+49754534 Msa0552960:CDS
GTATCCCCGCCTCTTCATCC+AGG 0.556309 4_1:+49755407 Msa0552960:CDS
TATGTATCCATGGCCTGCAA+TGG 0.558315 4_1:+49755193 Msa0552960:CDS
TAGTAGGGGTACGGCTGCAT+GGG 0.560037 4_1:-49755287 None:intergenic
AGCAGGTGTAATTTCCATTG+GGG 0.561289 4_1:-49754518 None:intergenic
ATAGTAGGGGTACGGCTGCA+TGG 0.562866 4_1:-49755288 None:intergenic
AACTAAGGACTATGTATCCA+TGG 0.564461 4_1:+49755183 Msa0552960:CDS
TTCTGCTGCAACTATTGTAG+AGG 0.565759 4_1:+49755878 Msa0552960:three_prime_UTR
CGGCTACCTGGATGAAGAGG+CGG 0.573692 4_1:-49755413 None:intergenic
GCATCAACCATAGATAGATA+AGG 0.576735 4_1:+49753914 Msa0552960:five_prime_UTR
ACCCAGGAGTCTTCAACTCA+AGG 0.576772 4_1:-49755529 None:intergenic
TAGTGTAGTGGGGAGCAGAA+AGG 0.578575 4_1:+49755779 Msa0552960:CDS
TAGCTCATCAAGTAGTGTAG+TGG 0.582065 4_1:+49755767 Msa0552960:CDS
GGTCTGAAGTTGAACCCCAA+TGG 0.587215 4_1:+49754504 Msa0552960:intron
TGATTAGCATAGTAGGGGTA+CGG 0.592321 4_1:-49755296 None:intergenic
AAACCTACATTTAGCCACAC+TGG 0.600095 4_1:-49756105 None:intergenic
TTAAGAAGAGAGAACAGAAG+CGG 0.601746 4_1:-49755936 None:intergenic
AAGGGAACATATGTTGAACA+AGG 0.603401 4_1:-49755341 None:intergenic
GTTGAACGTACGTCTGGACT+CGG 0.603755 4_1:+49756207 Msa0552960:three_prime_UTR
GACCGGCTACCTGGATGAAG+AGG 0.604106 4_1:-49755416 None:intergenic
GGAACTGGATACGGATATGT+TGG 0.606135 4_1:-49755239 None:intergenic
AAGGAAACCAGTTTCACAGA+AGG 0.607572 4_1:+49755702 Msa0552960:CDS
ACCTGATCTGCGGAAGACCC+AGG 0.614780 4_1:-49755545 None:intergenic
AGATACAGTTCAATTGCTGA+AGG 0.616836 4_1:+49754921 Msa0552960:CDS
TGTTGGTGGAGCCATCATAA+CGG 0.621484 4_1:-49755222 None:intergenic
CAAGGGTTAGGGATGACTGC+AGG 0.622814 4_1:-49755323 None:intergenic
AGGCGGGGATACGTATTGAG+TGG 0.624785 4_1:-49755396 None:intergenic
AAGACTAGATGAAATAAGCA+TGG 0.625916 4_1:-49756162 None:intergenic
GAGCAAGGCTCAAAGAAATG+AGG 0.629493 4_1:+49755484 Msa0552960:CDS
CTGACCGAGAAAAGTTAAGG+AGG 0.634700 4_1:+49754557 Msa0552960:CDS
AGCAGCACTAACCTGATCTG+CGG 0.637188 4_1:-49755555 None:intergenic
TGCATGGGAATTGAACCAGC+AGG 0.642923 4_1:-49755272 None:intergenic
GGATCACAGCAAGCAAACGA+CGG 0.646485 4_1:-49753767 None:intergenic
TGACCGAGAAAAGTTAAGGA+GGG 0.651662 4_1:+49754558 Msa0552960:CDS
GCTAAATGAAGAATCTCGTG+AGG 0.654605 4_1:+49754987 Msa0552960:CDS
TGTGAGGGAAAGGCTGCGTG+AGG 0.655698 4_1:+49756014 Msa0552960:three_prime_UTR
ACTGGATACGGATATGTTGG+TGG 0.657323 4_1:-49755236 None:intergenic
AGTCTTCAACTCAAGGTCTG+TGG 0.666361 4_1:-49755522 None:intergenic
GATGTTGATTAGCATAGTAG+GGG 0.671724 4_1:-49755301 None:intergenic
GCTACCTGGATGAAGAGGCG+GGG 0.673729 4_1:-49755411 None:intergenic
AGGGAACATATGTTGAACAA+GGG 0.680887 4_1:-49755340 None:intergenic
GCTCATCAAGTAGTGTAGTG+GGG 0.712201 4_1:+49755769 Msa0552960:CDS
TCTTCAAGTTGAGACCAGTG+TGG 0.762333 4_1:+49756091 Msa0552960:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTAATTATTTTGTGTTTT+TGG + chr4_1:49754459-49754478 Msa0552960:intron 10.0%
!!! AAAAAGCTCAAATTTTTAAA+AGG - chr4_1:49754047-49754066 None:intergenic 15.0%
!!! TATAAATTGATTCAAATCAA+TGG + chr4_1:49754213-49754232 Msa0552960:intron 15.0%
!!! TGAATTGTAATTGTGTTTTT+CGG - chr4_1:49753836-49753855 None:intergenic 20.0%
!!! ATCTTCTTCAATTTCAATTT+TGG + chr4_1:49753941-49753960 Msa0552960:intron 20.0%
!! TGTCAATTAAACAATAAAGA+TGG - chr4_1:49754009-49754028 None:intergenic 20.0%
!! TCTTTATTGTTTAATTGACA+TGG + chr4_1:49754009-49754028 Msa0552960:intron 20.0%
!! CTTTATTGTTTAATTGACAT+GGG + chr4_1:49754010-49754029 Msa0552960:intron 20.0%
!! CATTGAATCAAAGTAATTTA+GGG - chr4_1:49754091-49754110 None:intergenic 20.0%
!! ACATTGAATCAAAGTAATTT+AGG - chr4_1:49754092-49754111 None:intergenic 20.0%
!! ATTAGATTTCTTAGAAGTTA+GGG + chr4_1:49754275-49754294 Msa0552960:intron 20.0%
!!! CTTTCTTTTTTTGATCATTT+TGG + chr4_1:49754397-49754416 Msa0552960:intron 20.0%
!!! ATTTTGGTTGTTATTATTGA+AGG + chr4_1:49754413-49754432 Msa0552960:intron 20.0%
!!! TAATTGTGTTTTTCGGAAAA+AGG - chr4_1:49753829-49753848 None:intergenic 25.0%
! AACAAGAACAAGAATAAGAT+TGG + chr4_1:49753867-49753886 Msa0552960:five_prime_UTR 25.0%
! ACAAGAACAAGAATAAGATT+GGG + chr4_1:49753868-49753887 Msa0552960:five_prime_UTR 25.0%
!! GATTTTACCTTATCTATCTA+TGG - chr4_1:49753924-49753943 None:intergenic 25.0%
!!! ATGTACGAGTTTTGTATAAT+TGG + chr4_1:49754125-49754144 Msa0552960:intron 25.0%
! ATAATTGGTTAATCCCAAAT+TGG + chr4_1:49754140-49754159 Msa0552960:intron 25.0%
! AAACTCATACAATTCTCAAT+AGG - chr4_1:49754246-49754265 None:intergenic 25.0%
!! CTAACTTCTAAGAAATCTAA+TGG - chr4_1:49754276-49754295 None:intergenic 25.0%
! CATTAGATTTCTTAGAAGTT+AGG + chr4_1:49754274-49754293 Msa0552960:intron 25.0%
! TGTTGTTCAACAATTGTATT+CGG - chr4_1:49755374-49755393 None:intergenic 25.0%
!!! ATTAAAGCTGTTTTTGTTGT+CGG + chr4_1:49755628-49755647 Msa0552960:intron 25.0%
!!! CTTACTAGATTTTTTTTGTG+CGG - chr4_1:49755673-49755692 None:intergenic 25.0%
!! TATTTCATCTAGTCTTTGTT+TGG + chr4_1:49756169-49756188 Msa0552960:three_prime_UTR 25.0%
! CTTAGAAGTTAGGGTATTAT+TGG + chr4_1:49754284-49754303 Msa0552960:intron 30.0%
!! TTATTGGCTTCATAATGTGT+AGG + chr4_1:49754300-49754319 Msa0552960:intron 30.0%
! TTTTATGTGTTTACTGCGAA+AGG + chr4_1:49754483-49754502 Msa0552960:intron 30.0%
!!! AATGAACAGTTTTTAGAGCT+GGG + chr4_1:49754589-49754608 Msa0552960:CDS 30.0%
! TTTTCAACTACATGTTGCAA+AGG + chr4_1:49754704-49754723 Msa0552960:intron 30.0%
CTAGAATTCATTCATGTGTT+AGG + chr4_1:49754776-49754795 Msa0552960:intron 30.0%
!! TATCATTTTTAGGCCTATCA+GGG - chr4_1:49754885-49754904 None:intergenic 30.0%
!! TTATCATTTTTAGGCCTATC+AGG - chr4_1:49754886-49754905 None:intergenic 30.0%
!! ATGGTCGCTTTATCATTTTT+AGG - chr4_1:49754895-49754914 None:intergenic 30.0%
GTTTACTAACTTGAGAAGTA+AGG - chr4_1:49754948-49754967 None:intergenic 30.0%
! TTTGGGACATAAATTTACAG+AGG + chr4_1:49755048-49755067 Msa0552960:intron 30.0%
AAATGATCTCAGAGAAGAAA+AGG + chr4_1:49755112-49755131 Msa0552960:CDS 30.0%
ATTGTATTCGGAGCTAAAAA+GGG - chr4_1:49755362-49755381 None:intergenic 30.0%
AATTGTATTCGGAGCTAAAA+AGG - chr4_1:49755363-49755382 None:intergenic 30.0%
!!! TTTTTTGGACGAGAATAGAA+GGG - chr4_1:49755911-49755930 None:intergenic 30.0%
!!! AAACTTTTCTTTGAGTTGTG+AGG + chr4_1:49755998-49756017 Msa0552960:three_prime_UTR 30.0%
!!! AACTTTTCTTTGAGTTGTGA+GGG + chr4_1:49755999-49756018 Msa0552960:three_prime_UTR 30.0%
TTCAATTTGTCACAAGGAAT+AGG - chr4_1:49756056-49756075 None:intergenic 30.0%
TGCAATTTCAATTTGTCACA+AGG - chr4_1:49756062-49756081 None:intergenic 30.0%
TGACAAATTGAAATTGCAGA+TGG + chr4_1:49756064-49756083 Msa0552960:three_prime_UTR 30.0%
TTAGGAGAGTTTGTGATTAA+GGG - chr4_1:49756137-49756156 None:intergenic 30.0%
TTTAGGAGAGTTTGTGATTA+AGG - chr4_1:49756138-49756157 None:intergenic 30.0%
TAATCACAAACTCTCCTAAA+AGG + chr4_1:49756138-49756157 Msa0552960:three_prime_UTR 30.0%
AAGACTAGATGAAATAAGCA+TGG - chr4_1:49756165-49756184 None:intergenic 30.0%
GGAAAAAGGTGAAAAGTGAA+AGG - chr4_1:49753815-49753834 None:intergenic 35.0%
GCATCAACCATAGATAGATA+AGG + chr4_1:49753914-49753933 Msa0552960:five_prime_UTR 35.0%
!! AGGAACAAATTTTCAAGCAG+TGG + chr4_1:49754320-49754339 Msa0552960:intron 35.0%
!! AATTTTCAAGCAGTGGCTTT+TGG + chr4_1:49754327-49754346 Msa0552960:intron 35.0%
TAGCAGGTGTAATTTCCATT+GGG - chr4_1:49754522-49754541 None:intergenic 35.0%
!!! CAATGAACAGTTTTTAGAGC+TGG + chr4_1:49754588-49754607 Msa0552960:CDS 35.0%
! TTAGAGCTGGGTAACATTTT+AGG + chr4_1:49754601-49754620 Msa0552960:CDS 35.0%
AATGATAAAGCGACCATTCT+AGG + chr4_1:49754898-49754917 Msa0552960:CDS 35.0%
TTGAACTGTATCTCCTAGAA+TGG - chr4_1:49754914-49754933 None:intergenic 35.0%
AGATACAGTTCAATTGCTGA+AGG + chr4_1:49754921-49754940 Msa0552960:CDS 35.0%
! CGTGAGGTAATATTTTCTCT+CGG + chr4_1:49755003-49755022 Msa0552960:intron 35.0%
! ATGAATCACCTTGATGCTTT+TGG + chr4_1:49755030-49755049 Msa0552960:intron 35.0%
! TGAATCACCTTGATGCTTTT+GGG + chr4_1:49755031-49755050 Msa0552960:intron 35.0%
!! TTTATGTCCCAAAAGCATCA+AGG - chr4_1:49755041-49755060 None:intergenic 35.0%
AACTAAGGACTATGTATCCA+TGG + chr4_1:49755183-49755202 Msa0552960:CDS 35.0%
AATGGATCATTCCGTTATGA+TGG + chr4_1:49755211-49755230 Msa0552960:CDS 35.0%
! GATGTTGATTAGCATAGTAG+GGG - chr4_1:49755304-49755323 None:intergenic 35.0%
! GGATGTTGATTAGCATAGTA+GGG - chr4_1:49755305-49755324 None:intergenic 35.0%
! AGGATGTTGATTAGCATAGT+AGG - chr4_1:49755306-49755325 None:intergenic 35.0%
CATATGTTGAACAAGGGTTA+GGG - chr4_1:49755337-49755356 None:intergenic 35.0%
ACATATGTTGAACAAGGGTT+AGG - chr4_1:49755338-49755357 None:intergenic 35.0%
AGGGAACATATGTTGAACAA+GGG - chr4_1:49755343-49755362 None:intergenic 35.0%
AAGGGAACATATGTTGAACA+AGG - chr4_1:49755344-49755363 None:intergenic 35.0%
ACTCCAAAAACAAATCATCC+AGG + chr4_1:49755459-49755478 Msa0552960:CDS 35.0%
CTCCAAAAACAAATCATCCA+GGG + chr4_1:49755460-49755479 Msa0552960:CDS 35.0%
!!! GTTAGTGCTGCTTTTTTCTT+GGG + chr4_1:49755566-49755585 Msa0552960:intron 35.0%
!! TTTTTGTTGTCGGATTTTGC+AGG + chr4_1:49755638-49755657 Msa0552960:intron 35.0%
AAAAATCTAGTAAGTCGTCG+AGG + chr4_1:49755679-49755698 Msa0552960:CDS 35.0%
!!! CTTTTTTGGACGAGAATAGA+AGG - chr4_1:49755912-49755931 None:intergenic 35.0%
TTAAGAAGAGAGAACAGAAG+CGG - chr4_1:49755939-49755958 None:intergenic 35.0%
TCTGTTCTCTCTTCTTAACT+AGG + chr4_1:49755941-49755960 Msa0552960:three_prime_UTR 35.0%
! GAAATAAGCATGGTCCTTTT+AGG - chr4_1:49756155-49756174 None:intergenic 35.0%
!! TAGTGGAAGAGTTGTCGTTT+TGG - chr4_1:49753791-49753810 None:intergenic 40.0%
GGTGAAAAGTGAAAGGATAG+TGG - chr4_1:49753808-49753827 None:intergenic 40.0%
GAACTACACAGCACCAATTT+GGG - chr4_1:49754156-49754175 None:intergenic 40.0%
TGAACTACACAGCACCAATT+TGG - chr4_1:49754157-49754176 None:intergenic 40.0%
AGCAGGTGTAATTTCCATTG+GGG - chr4_1:49754521-49754540 None:intergenic 40.0%
CTAGCAGGTGTAATTTCCAT+TGG - chr4_1:49754523-49754542 None:intergenic 40.0%
ACCTGCTAGAAAGACACAAA+AGG + chr4_1:49754534-49754553 Msa0552960:CDS 40.0%
TGACCGAGAAAAGTTAAGGA+GGG + chr4_1:49754558-49754577 Msa0552960:CDS 40.0%
! GATCCCTCCTTAACTTTTCT+CGG - chr4_1:49754564-49754583 None:intergenic 40.0%
AGAGCACAGAGTAATTCAAG+AGG - chr4_1:49754841-49754860 None:intergenic 40.0%
TTGAATTACTCTGTGCTCTG+TGG + chr4_1:49754842-49754861 Msa0552960:intron 40.0%
GCTAAATGAAGAATCTCGTG+AGG + chr4_1:49754987-49755006 Msa0552960:CDS 40.0%
GTTCAATTTCCAGTTGTCTC+AGG + chr4_1:49755085-49755104 Msa0552960:intron 40.0%
! CATTTTTCTCCTGAGACAAC+TGG - chr4_1:49755097-49755116 None:intergenic 40.0%
! ATAACGGAATGATCCATTGC+AGG - chr4_1:49755209-49755228 None:intergenic 40.0%
TGATTAGCATAGTAGGGGTA+CGG - chr4_1:49755299-49755318 None:intergenic 40.0%
!!! GTCTTGTTTTCCTGATACGT+GGG - chr4_1:49755441-49755460 None:intergenic 40.0%
!!! AGTCTTGTTTTCCTGATACG+TGG - chr4_1:49755442-49755461 None:intergenic 40.0%
!!! CTCCCTGGATGATTTGTTTT+TGG - chr4_1:49755465-49755484 None:intergenic 40.0%
!!! GGTTAGTGCTGCTTTTTTCT+TGG + chr4_1:49755565-49755584 Msa0552960:intron 40.0%
ACCTATAGATGCAGTAGAAG+AGG + chr4_1:49755599-49755618 Msa0552960:intron 40.0%
ACCTCTTCTACTGCATCTAT+AGG - chr4_1:49755603-49755622 None:intergenic 40.0%
AAGGAAACCAGTTTCACAGA+AGG + chr4_1:49755702-49755721 Msa0552960:CDS 40.0%
TTCACAGAAGGTAGTTCGTT+AGG + chr4_1:49755714-49755733 Msa0552960:CDS 40.0%
TTCTTCATCTCACAGTGTTC+AGG + chr4_1:49755743-49755762 Msa0552960:CDS 40.0%
TAGCTCATCAAGTAGTGTAG+TGG + chr4_1:49755767-49755786 Msa0552960:CDS 40.0%
AGCTCATCAAGTAGTGTAGT+GGG + chr4_1:49755768-49755787 Msa0552960:CDS 40.0%
GAAAGGCTAGTGAATGAGAA+TGG + chr4_1:49755796-49755815 Msa0552960:exon 40.0%
! CTACTGTGAGGGTTTTCATT+GGG + chr4_1:49755854-49755873 Msa0552960:three_prime_UTR 40.0%
TTCTGCTGCAACTATTGTAG+AGG + chr4_1:49755878-49755897 Msa0552960:three_prime_UTR 40.0%
!!! ACAGAAGCGGCTTTCTTTTT+TGG - chr4_1:49755926-49755945 None:intergenic 40.0%
!! TTCTTTGAGTTGTGAGGGAA+AGG + chr4_1:49756004-49756023 Msa0552960:three_prime_UTR 40.0%
AAACCTACATTTAGCCACAC+TGG - chr4_1:49756108-49756127 None:intergenic 40.0%
GAAAATGTTGAACGTACGTC+TGG + chr4_1:49756201-49756220 Msa0552960:three_prime_UTR 40.0%
! GTGGCTTTTGGACATGTCAA+AGG + chr4_1:49754339-49754358 Msa0552960:intron 45.0%
!! GCCTTTTGTGTCTTTCTAGC+AGG - chr4_1:49754538-49754557 None:intergenic 45.0%
AGGCTGACCGAGAAAAGTTA+AGG + chr4_1:49754554-49754573 Msa0552960:CDS 45.0%
CTGACCGAGAAAAGTTAAGG+AGG + chr4_1:49754557-49754576 Msa0552960:CDS 45.0%
! TCTGAAATGCAGACCCTGAT+AGG + chr4_1:49754869-49754888 Msa0552960:intron 45.0%
ACCAGTATCAGCTGCAACTA+AGG + chr4_1:49755168-49755187 Msa0552960:CDS 45.0%
TCCTTAGTTGCAGCTGATAC+TGG - chr4_1:49755172-49755191 None:intergenic 45.0%
TATGTATCCATGGCCTGCAA+TGG + chr4_1:49755193-49755212 Msa0552960:CDS 45.0%
! TGTTGGTGGAGCCATCATAA+CGG - chr4_1:49755225-49755244 None:intergenic 45.0%
ACTGGATACGGATATGTTGG+TGG - chr4_1:49755239-49755258 None:intergenic 45.0%
GGAACTGGATACGGATATGT+TGG - chr4_1:49755242-49755261 None:intergenic 45.0%
GAGCAAGGCTCAAAGAAATG+AGG + chr4_1:49755484-49755503 Msa0552960:CDS 45.0%
AGTCTTCAACTCAAGGTCTG+TGG - chr4_1:49755525-49755544 None:intergenic 45.0%
!! ACCTTGAGTTGAAGACTCCT+GGG + chr4_1:49755528-49755547 Msa0552960:CDS 45.0%
ATCTAGTAAGTCGTCGAGGA+AGG + chr4_1:49755683-49755702 Msa0552960:CDS 45.0%
CGAACTACCTTCTGTGAAAC+TGG - chr4_1:49755712-49755731 None:intergenic 45.0%
GCTCATCAAGTAGTGTAGTG+GGG + chr4_1:49755769-49755788 Msa0552960:CDS 45.0%
!!! TGCCTTTTGAAGCTACTGTG+AGG + chr4_1:49755842-49755861 Msa0552960:three_prime_UTR 45.0%
!!! GCCTTTTGAAGCTACTGTGA+GGG + chr4_1:49755843-49755862 Msa0552960:three_prime_UTR 45.0%
ACCCTCACAGTAGCTTCAAA+AGG - chr4_1:49755847-49755866 None:intergenic 45.0%
! GCTACTGTGAGGGTTTTCAT+TGG + chr4_1:49755853-49755872 Msa0552960:three_prime_UTR 45.0%
!! TCTTCAAGTTGAGACCAGTG+TGG + chr4_1:49756091-49756110 Msa0552960:three_prime_UTR 45.0%
AGACCAGTGTGGCTAAATGT+AGG + chr4_1:49756102-49756121 Msa0552960:three_prime_UTR 45.0%
GGATCACAGCAAGCAAACGA+CGG - chr4_1:49753770-49753789 None:intergenic 50.0%
! GGTCTGAAGTTGAACCCCAA+TGG + chr4_1:49754504-49754523 Msa0552960:intron 50.0%
CAAAACGTGAGCAGAGTCAC+TGG + chr4_1:49754659-49754678 Msa0552960:intron 50.0%
GAATGATCCATTGCAGGCCA+TGG - chr4_1:49755203-49755222 None:intergenic 50.0%
TCCGTATCCAGTTCCAATGC+CGG + chr4_1:49755247-49755266 Msa0552960:CDS 50.0%
! ACCGGCATTGGAACTGGATA+CGG - chr4_1:49755251-49755270 None:intergenic 50.0%
GGAATTGAACCAGCAGGTAC+CGG - chr4_1:49755269-49755288 None:intergenic 50.0%
TGCATGGGAATTGAACCAGC+AGG - chr4_1:49755275-49755294 None:intergenic 50.0%
! GTTTTCCTGATACGTGGGAC+CGG - chr4_1:49755436-49755455 None:intergenic 50.0%
CAAATCATCCAGGGAGAGCA+AGG + chr4_1:49755469-49755488 Msa0552960:CDS 50.0%
!! GACCTTGAGTTGAAGACTCC+TGG + chr4_1:49755527-49755546 Msa0552960:CDS 50.0%
ACCCAGGAGTCTTCAACTCA+AGG - chr4_1:49755532-49755551 None:intergenic 50.0%
AGCAGCACTAACCTGATCTG+CGG - chr4_1:49755558-49755577 None:intergenic 50.0%
! TAGTGTAGTGGGGAGCAGAA+AGG + chr4_1:49755779-49755798 Msa0552960:CDS 50.0%
GTTGAACGTACGTCTGGACT+CGG + chr4_1:49756207-49756226 Msa0552960:three_prime_UTR 50.0%
! TAGTAGGGGTACGGCTGCAT+GGG - chr4_1:49755290-49755309 None:intergenic 55.0%
! ATAGTAGGGGTACGGCTGCA+TGG - chr4_1:49755291-49755310 None:intergenic 55.0%
CAAGGGTTAGGGATGACTGC+AGG - chr4_1:49755326-49755345 None:intergenic 55.0%
AGGCGGGGATACGTATTGAG+TGG - chr4_1:49755399-49755418 None:intergenic 55.0%
TCTTTGAGCCTTGCTCTCCC+TGG - chr4_1:49755480-49755499 None:intergenic 55.0%
!! GCAGGTACCGGCATTGGAAC+TGG - chr4_1:49755257-49755276 None:intergenic 60.0%
GTTCCAATGCCGGTACCTGC+TGG + chr4_1:49755257-49755276 Msa0552960:CDS 60.0%
!! GAACCAGCAGGTACCGGCAT+TGG - chr4_1:49755263-49755282 None:intergenic 60.0%
GTATCCCCGCCTCTTCATCC+AGG + chr4_1:49755407-49755426 Msa0552960:CDS 60.0%
GCTACCTGGATGAAGAGGCG+GGG - chr4_1:49755414-49755433 None:intergenic 60.0%
GGCTACCTGGATGAAGAGGC+GGG - chr4_1:49755415-49755434 None:intergenic 60.0%
CGGCTACCTGGATGAAGAGG+CGG - chr4_1:49755416-49755435 None:intergenic 60.0%
CGCCTCTTCATCCAGGTAGC+CGG + chr4_1:49755414-49755433 Msa0552960:CDS 60.0%
GACCGGCTACCTGGATGAAG+AGG - chr4_1:49755419-49755438 None:intergenic 60.0%
TCCTGGGTCTTCCGCAGATC+AGG + chr4_1:49755544-49755563 Msa0552960:CDS 60.0%
ACCTGATCTGCGGAAGACCC+AGG - chr4_1:49755548-49755567 None:intergenic 60.0%
TGTGAGGGAAAGGCTGCGTG+AGG + chr4_1:49756014-49756033 Msa0552960:three_prime_UTR 60.0%
GATACGTGGGACCGGCTACC+TGG - chr4_1:49755428-49755447 None:intergenic 65.0%
GGTAGCCGGTCCCACGTATC+AGG + chr4_1:49755428-49755447 Msa0552960:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 49753724 49756235 49753724 ID=Msa0552960;Name=Msa0552960
chr4_1 mRNA 49753724 49756235 49753724 ID=Msa0552960-mRNA-1;Parent=Msa0552960;Name=Msa0552960-mRNA-1;_AED=0.11;_eAED=0.11;_QI=230|1|1|1|0.75|0.6|5|423|282
chr4_1 exon 49753724 49753935 49753724 ID=Msa0552960-mRNA-1:exon:13984;Parent=Msa0552960-mRNA-1
chr4_1 exon 49754505 49754622 49754505 ID=Msa0552960-mRNA-1:exon:13985;Parent=Msa0552960-mRNA-1
chr4_1 exon 49754881 49755008 49754881 ID=Msa0552960-mRNA-1:exon:13986;Parent=Msa0552960-mRNA-1
chr4_1 exon 49755098 49755565 49755098 ID=Msa0552960-mRNA-1:exon:13987;Parent=Msa0552960-mRNA-1
chr4_1 exon 49755660 49756235 49755660 ID=Msa0552960-mRNA-1:exon:13988;Parent=Msa0552960-mRNA-1
chr4_1 five_prime_UTR 49753724 49753935 49753724 ID=Msa0552960-mRNA-1:five_prime_utr;Parent=Msa0552960-mRNA-1
chr4_1 five_prime_UTR 49754505 49754522 49754505 ID=Msa0552960-mRNA-1:five_prime_utr;Parent=Msa0552960-mRNA-1
chr4_1 CDS 49754523 49754622 49754523 ID=Msa0552960-mRNA-1:cds;Parent=Msa0552960-mRNA-1
chr4_1 CDS 49754881 49755008 49754881 ID=Msa0552960-mRNA-1:cds;Parent=Msa0552960-mRNA-1
chr4_1 CDS 49755098 49755565 49755098 ID=Msa0552960-mRNA-1:cds;Parent=Msa0552960-mRNA-1
chr4_1 CDS 49755660 49755812 49755660 ID=Msa0552960-mRNA-1:cds;Parent=Msa0552960-mRNA-1
chr4_1 three_prime_UTR 49755813 49756235 49755813 ID=Msa0552960-mRNA-1:three_prime_utr;Parent=Msa0552960-mRNA-1
Gene Sequence

>Msa0552960

ATGGAAATTACACCTGCTAGAAAGACACAAAAGGCTGACCGAGAAAAGTTAAGGAGGGATCGACTCAATGAACAGTTTTTAGAGCTGGGTAACATTTTAGACCCTGATAGGCCTAAAAATGATAAAGCGACCATTCTAGGAGATACAGTTCAATTGCTGAAGGACCTTACTTCTCAAGTTAGTAAACTAAAAGATGAATATACTATGCTAAATGAAGAATCTCGTGAGTTGTCTCAGGAGAAAAATGATCTCAGAGAAGAAAAGGCTTCTCTTAAATCAGATATTGAGAATTTGAATAACCAGTATCAGCTGCAACTAAGGACTATGTATCCATGGCCTGCAATGGATCATTCCGTTATGATGGCTCCACCAACATATCCGTATCCAGTTCCAATGCCGGTACCTGCTGGTTCAATTCCCATGCAGCCGTACCCCTACTATGCTAATCAACATCCTGCAGTCATCCCTAACCCTTGTTCAACATATGTTCCCTTTTTAGCTCCGAATACAATTGTTGAACAACAATCCACTCAATACGTATCCCCGCCTCTTCATCCAGGTAGCCGGTCCCACGTATCAGGAAAACAAGACTCCAAAAACAAATCATCCAGGGAGAGCAAGGCTCAAAGAAATGAGGATTCTAATGATGTAGCCACAGACCTTGAGTTGAAGACTCCTGGGTCTTCCGCAGATCAGGATTTAACATCCGCACAAAAAAAATCTAGTAAGTCGTCGAGGAAGGAAACCAGTTTCACAGAAGGTAGTTCGTTAGGCAAGTGTTCTTCATCTCACAGTGTTCAGGATAGCTCATCAAGTAGTGTAGTGGGGAGCAGAAAGGCTAGTGAATGA
Protein sequence

>Msa0552960

MEITPARKTQKADREKLRRDRLNEQFLELGNILDPDRPKNDKATILGDTVQLLKDLTSQVSKLKDEYTMLNEESRELSQEKNDLREEKASLKSDIENLNNQYQLQLRTMYPWPAMDHSVMMAPPTYPYPVPMPVPAGSIPMQPYPYYANQHPAVIPNPCSTYVPFLAPNTIVEQQSTQYVSPPLHPGSRSHVSGKQDSKNKSSRESKAQRNEDSNDVATDLELKTPGSSADQDLTSAQKKSSKSSRKETSFTEGSSLGKCSSSHSVQDSSSSSVVGSRKASE*