AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0553840

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0553840 MtrunA17_Chr4g0039661 97.482 556 13 1 14 569 1 555 0.0 1109
Msa0553840 MtrunA17_Chr2g0319091 38.462 351 191 12 233 568 238 578 2.77e-54 193
Msa0553840 MtrunA17_Chr3g0101141 36.158 354 209 11 227 569 195 542 1.32e-53 191
Msa0553840 MtrunA17_Chr5g0445331 34.545 385 231 10 200 569 154 532 5.69e-53 189
Msa0553840 MtrunA17_Chr3g0110971 35.401 387 214 13 204 569 216 587 5.14e-52 187
Msa0553840 MtrunA17_Chr7g0245601 33.600 375 211 11 210 569 441 792 7.10e-52 190
Msa0553840 MtrunA17_Chr7g0224271 31.529 425 235 11 170 569 280 673 8.26e-51 185
Msa0553840 MtrunA17_Chr5g0447691 33.947 380 236 9 200 569 170 544 1.74e-50 182
Msa0553840 MtrunA17_Chr4g0038761 33.967 368 198 12 221 569 101 442 2.03e-50 180
Msa0553840 MtrunA17_Chr3g0125811 32.095 377 231 10 208 569 204 570 2.15e-50 182
Msa0553840 MtrunA17_Chr1g0183641 34.225 374 220 11 204 568 120 476 5.13e-50 179
Msa0553840 MtrunA17_Chr1g0159461 31.989 372 238 8 208 569 226 592 2.09e-49 180
Msa0553840 MtrunA17_Chr2g0306641 32.120 467 263 19 120 569 58 487 2.75e-49 178
Msa0553840 MtrunA17_Chr3g0114841 31.536 371 232 7 217 568 132 499 5.83e-44 163
Msa0553840 MtrunA17_Chr4g0057291 30.287 383 239 12 204 568 289 661 1.56e-43 165
Msa0553840 MtrunA17_Chr4g0076091 32.857 350 218 10 230 568 199 542 3.20e-43 162
Msa0553840 MtrunA17_Chr4g0057891 27.792 385 249 7 204 569 146 520 8.22e-43 160
Msa0553840 MtrunA17_Chr8g0376381 30.952 378 228 14 204 569 166 522 1.58e-42 160
Msa0553840 MtrunA17_Chr1g0200401 31.392 395 232 11 203 569 76 459 6.79e-42 157
Msa0553840 MtrunA17_Chr2g0290241 31.143 350 226 9 230 569 81 425 9.01e-42 155
Msa0553840 MtrunA17_Chr5g0424131 31.519 349 222 6 235 568 152 498 4.89e-40 152
Msa0553840 MtrunA17_Chr3g0085681 27.714 433 239 13 198 569 17 436 1.06e-37 144
Msa0553840 MtrunA17_Chr2g0328341 28.684 380 244 10 205 568 269 637 4.85e-37 145
Msa0553840 MtrunA17_Chr4g0031741 27.676 383 255 9 199 568 276 649 3.51e-36 143
Msa0553840 MtrunA17_Chr2g0328391 26.772 381 254 10 205 568 296 668 4.21e-36 143
Msa0553840 MtrunA17_Chr4g0031721 29.024 379 247 9 204 568 313 683 5.94e-36 142
Msa0553840 MtrunA17_Chr2g0328381 28.684 380 244 10 205 568 272 640 1.88e-35 141
Msa0553840 MtrunA17_Chr4g0031711 27.473 364 238 9 223 568 377 732 2.10e-35 141
Msa0553840 MtrunA17_Chr5g0396491 25.858 437 242 10 204 569 53 478 2.61e-35 138
Msa0553840 MtrunA17_Chr5g0401131 29.771 393 240 13 200 569 111 490 5.68e-35 137
Msa0553840 MtrunA17_Chr8g0357601 28.571 385 245 11 204 569 179 552 1.07e-34 138
Msa0553840 MtrunA17_Chr2g0294461 27.062 388 252 11 204 569 207 585 1.77e-34 137
Msa0553840 MtrunA17_Chr2g0328411 25.729 377 259 7 204 568 286 653 2.98e-34 137
Msa0553840 MtrunA17_Chr4g0031701 25.693 397 277 8 176 558 223 615 7.87e-34 136
Msa0553840 MtrunA17_Chr4g0038851 26.897 435 239 12 204 569 45 469 8.85e-34 134
Msa0553840 MtrunA17_Chr2g0294471 26.579 380 261 8 204 569 201 576 1.04e-33 135
Msa0553840 MtrunA17_Chr8g0383981 30.479 397 246 15 184 568 117 495 1.23e-33 134
Msa0553840 MtrunA17_Chr4g0031731 27.714 350 235 8 223 558 270 615 2.39e-33 134
Msa0553840 MtrunA17_Chr2g0328431 25.729 377 259 7 204 568 286 653 9.64e-33 133
Msa0553840 MtrunA17_Chr4g0012071 29.639 415 240 16 177 569 167 551 1.02e-32 132
Msa0553840 MtrunA17_Chr2g0328401 25.905 359 247 6 188 534 271 622 3.60e-32 131
Msa0553840 MtrunA17_Chr7g0238891 26.836 354 241 8 230 569 391 740 3.61e-32 132
Msa0553840 MtrunA17_Chr7g0270031 29.076 368 244 10 212 569 194 554 9.08e-32 129
Msa0553840 MtrunA17_Chr2g0328361 28.342 374 228 11 205 568 271 614 1.59e-31 129
Msa0553840 MtrunA17_Chr7g0230771 30.667 375 236 12 204 569 372 731 9.72e-31 127
Msa0553840 MtrunA17_Chr3g0085361 27.714 350 184 10 277 569 13 350 5.71e-30 120
Msa0553840 MtrunA17_Chr7g0230731 29.867 375 239 11 204 569 369 728 8.17e-30 124
Msa0553840 MtrunA17_Chr2g0328371 29.845 258 158 7 275 519 1 248 3.33e-28 113
Msa0553840 MtrunA17_Chr2g0329591 27.481 393 242 11 206 569 73 451 4.64e-28 117
Msa0553840 MtrunA17_Chr7g0236851 29.017 417 256 16 172 568 260 656 3.32e-23 103
Msa0553840 MtrunA17_Chr5g0401421 24.201 438 264 12 194 568 13 445 6.40e-23 102
Msa0553840 MtrunA17_Chr4g0053901 23.894 452 275 15 171 568 31 467 1.36e-19 92.0
Msa0553840 MtrunA17_Chr8g0344101 25.260 384 258 10 208 569 224 600 8.05e-19 90.1
Msa0553840 MtrunA17_Chr1g0194621 21.271 362 251 10 229 569 170 518 1.59e-18 89.0
Msa0553840 MtrunA17_Chr8g0373561 25.490 357 218 13 235 569 211 541 3.37e-15 78.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0553840 AT5G66770.1 54.441 608 201 15 14 569 1 584 0.0 533
Msa0553840 AT3G50650.1 49.829 584 223 14 14 569 1 542 6.87e-162 473
Msa0553840 AT5G41920.1 33.007 409 231 13 180 569 16 400 1.56e-54 190
Msa0553840 AT5G48150.1 34.646 381 233 9 200 569 115 490 3.53e-54 191
Msa0553840 AT5G48150.4 34.646 381 233 9 200 569 115 490 3.53e-54 191
Msa0553840 AT5G48150.2 34.646 381 233 9 200 569 115 490 3.53e-54 191
Msa0553840 AT5G48150.3 34.646 381 233 9 200 569 115 490 3.53e-54 191
Msa0553840 AT1G50600.3 35.000 380 232 9 200 569 152 526 2.18e-53 190
Msa0553840 AT1G50600.1 34.474 380 234 8 200 569 223 597 1.20e-52 189
Msa0553840 AT1G50600.2 35.000 380 232 9 200 569 227 601 1.27e-52 189
Msa0553840 AT3G03450.1 34.218 377 226 13 204 569 180 545 1.57e-52 188
Msa0553840 AT3G54220.1 33.784 370 218 11 210 568 296 649 3.99e-52 189
Msa0553840 AT2G04890.1 33.803 355 211 12 226 569 72 413 5.17e-48 172
Msa0553840 AT5G17490.1 31.980 394 243 13 187 569 137 516 6.44e-46 169
Msa0553840 AT1G21450.1 28.750 400 270 9 180 569 199 593 4.61e-45 168
Msa0553840 AT1G66350.1 28.936 470 277 16 122 569 72 506 3.00e-44 164
Msa0553840 AT1G63100.1 31.776 321 184 8 270 568 321 628 6.16e-43 163
Msa0553840 AT1G63100.2 31.776 321 184 8 270 568 346 653 8.22e-43 163
Msa0553840 AT4G17230.1 32.181 376 240 9 204 569 155 525 1.74e-42 160
Msa0553840 AT4G17230.2 32.181 376 240 9 204 569 155 525 1.74e-42 160
Msa0553840 AT2G01570.1 33.333 348 204 15 233 569 251 581 1.79e-41 158
Msa0553840 AT1G55580.1 31.579 380 205 9 237 569 74 445 2.73e-41 155
Msa0553840 AT1G14920.1 31.519 349 209 13 233 569 199 529 4.65e-40 153
Msa0553840 AT3G46600.2 27.493 371 256 7 208 569 5 371 1.06e-38 146
Msa0553840 AT4G08250.1 29.429 350 237 5 227 568 129 476 2.73e-38 147
Msa0553840 AT3G46600.1 27.493 371 256 7 208 569 213 579 1.18e-37 147
Msa0553840 AT4G37650.1 29.101 378 238 11 216 569 158 529 1.20e-37 146
Msa0553840 AT3G46600.3 27.493 371 256 7 208 569 181 547 1.28e-37 147
Msa0553840 AT1G07520.2 28.382 377 251 7 208 568 155 528 2.08e-37 145
Msa0553840 AT1G07520.4 28.382 377 251 7 208 568 155 528 2.08e-37 145
Msa0553840 AT1G07520.3 28.382 377 251 7 208 568 320 693 2.35e-37 147
Msa0553840 AT1G07520.1 28.382 377 251 7 208 568 319 692 2.74e-37 147
Msa0553840 AT5G59450.1 26.303 403 279 8 181 569 200 598 9.41e-37 145
Msa0553840 AT1G07530.1 26.633 398 270 9 185 568 375 764 5.64e-36 144
Msa0553840 AT2G29065.1 27.763 389 239 10 208 568 253 627 9.16e-36 142
Msa0553840 AT2G29060.1 24.524 420 300 9 162 568 276 691 1.34e-34 139
Msa0553840 AT2G29060.2 24.524 420 300 9 162 568 239 654 1.39e-34 139
Msa0553840 AT2G37650.1 24.854 342 241 6 238 569 378 713 1.10e-27 118
Msa0553840 AT2G37650.2 24.854 342 241 6 238 569 366 701 1.26e-27 118
Msa0553840 AT5G52510.1 26.615 387 248 12 206 569 267 640 5.93e-26 112
Msa0553840 AT4G00150.1 28.338 367 224 13 219 569 211 554 7.13e-26 112
Msa0553840 AT1G50420.1 31.278 227 139 5 218 438 71 286 1.29e-23 104
Msa0553840 AT1G50420.1 32.414 145 90 3 430 569 338 479 7.65e-15 77.8
Msa0553840 AT2G45160.1 28.070 399 227 16 204 569 268 639 1.53e-23 105
Msa0553840 AT3G60630.1 29.412 306 190 9 278 569 329 622 2.26e-23 104
Msa0553840 AT3G49950.1 27.171 357 226 12 237 568 60 407 1.11e-22 101
Msa0553840 AT3G13840.1 25.459 381 250 9 206 569 147 510 1.18e-20 95.9
Msa0553840 AT4G36710.1 23.622 381 259 13 204 569 122 485 9.97e-17 83.6

Find 151 sgRNAs with CRISPR-Local

Find 284 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAATAGTGTAAGAATTTC+TGG 0.119456 4_1:-51122491 Msa0553840:CDS
GATATTCATATAGTTGATTT+TGG 0.147557 4_1:-51122578 Msa0553840:CDS
CTCTATTGACTGTTTCTTCT+TGG 0.170351 4_1:-51121821 Msa0553840:CDS
CCAGAAATTCTTACACTATT+AGG 0.205458 4_1:+51122491 None:intergenic
GCTTCAGTTGTATAATCTTT+TGG 0.233520 4_1:-51122282 Msa0553840:CDS
TAAAAGGAAACTCTTTGATT+AGG 0.234171 4_1:+51122785 None:intergenic
CTCTGTAAATTTGATTTGTA+TGG 0.250531 4_1:-51121359 Msa0553840:three_prime_UTR
GGTATACCTGCTATGGCTTT+AGG 0.252034 4_1:-51122470 Msa0553840:CDS
TCACCTAAAGTAACAATCTT+AGG 0.256338 4_1:+51122206 None:intergenic
AAATGTGCGAACTTTGAATA+TGG 0.257358 4_1:+51122644 None:intergenic
TGTGGAGTGGGTGGTTGAAT+TGG 0.280637 4_1:+51122977 None:intergenic
AAAAGGAAACTCTTTGATTA+GGG 0.285344 4_1:+51122786 None:intergenic
TTGATGATATTGGCTCAAAT+TGG 0.286520 4_1:-51121791 Msa0553840:three_prime_UTR
AATCCTAAGATTGTTACTTT+AGG 0.287104 4_1:-51122209 Msa0553840:CDS
GTTGATGTTGCACTGGTATT+AGG 0.301823 4_1:-51121467 Msa0553840:three_prime_UTR
AAGGTTATGGACTTGTAAAA+TGG 0.302281 4_1:-51121431 Msa0553840:three_prime_UTR
AGGGTTTCAATTGCTTGATT+TGG 0.313945 4_1:+51122845 None:intergenic
TATTTGTCTAAATATCAAAA+AGG 0.329898 4_1:-51121329 Msa0553840:three_prime_UTR
TAGGGCTTGTGAAAAGTAAA+AGG 0.330479 4_1:+51122769 None:intergenic
TAATTAAATTAATTATCGTT+TGG 0.333699 4_1:-51121582 Msa0553840:three_prime_UTR
TGGGTGGTTGAATTGGGATT+TGG 0.334521 4_1:+51122984 None:intergenic
TGGCGTTGATTTGATGATAT+TGG 0.335085 4_1:-51121801 Msa0553840:exon
AATTAAATTAATTATCGTTT+GGG 0.335442 4_1:-51121581 Msa0553840:three_prime_UTR
TTAAAGAAGCGATTTCTGTT+AGG 0.340571 4_1:+51122882 None:intergenic
GGATTCGCCGGAGAGGTTTC+AGG 0.345000 4_1:-51122078 Msa0553840:CDS
CTTGTTGAGCTATTGCCATT+AGG 0.345192 4_1:+51123434 None:intergenic
GTGGAGTGGGTGGTTGAATT+GGG 0.346709 4_1:+51122978 None:intergenic
TCGATTTACTGAGATGGGTT+AGG 0.351223 4_1:+51122825 None:intergenic
TTGTGATACCTAGGATGTTT+TGG 0.353661 4_1:+51123347 None:intergenic
GCATTTGCAACTCGTTCTTC+TGG 0.357528 4_1:-51122518 Msa0553840:CDS
TTAACAGCTAAAGCCTCATT+TGG 0.363944 4_1:+51122311 None:intergenic
TTGAAATGGGTCGGTGAAAT+CGG 0.367983 4_1:+51123231 None:intergenic
GGACCCGACCCGACTTGAAA+TGG 0.372343 4_1:+51123217 None:intergenic
CAAATTCTTGTTCTACTACA+TGG 0.381946 4_1:-51122709 Msa0553840:CDS
TAGTTCTTTGTATTCTCTTG+TGG 0.398410 4_1:-51121886 Msa0553840:CDS
TGAAAACAAAAGAAAGGAAA+AGG 0.401725 4_1:+51121522 None:intergenic
CGATTTACTGAGATGGGTTA+GGG 0.403048 4_1:+51122826 None:intergenic
CAAAGCAGTGATCAAGGTTA+TGG 0.404632 4_1:-51121444 Msa0553840:three_prime_UTR
TGAAAACGACGATGTCGTTT+CGG 0.406416 4_1:+51122931 None:intergenic
TTGCTTGGTAGGAGAATTGA+TGG 0.408569 4_1:-51122044 Msa0553840:CDS
GAATTGTTCAAGGGATTCAA+TGG 0.414040 4_1:-51122556 Msa0553840:CDS
GATTCATCAAGAAGCTCAAT+AGG 0.416164 4_1:+51122350 None:intergenic
TAAAGAAGCGATTTCTGTTA+GGG 0.419829 4_1:+51122883 None:intergenic
CTATGCAGATTGGATATGTT+TGG 0.419938 4_1:-51121646 Msa0553840:three_prime_UTR
TATTGGCTCAAATTGGGAAA+AGG 0.422482 4_1:-51121784 Msa0553840:three_prime_UTR
CCGATTTCGACTCCGACGAC+TGG 0.423487 4_1:-51123126 Msa0553840:CDS
GACCCGACTCTCACCACAAC+CGG 0.424081 4_1:-51123035 Msa0553840:CDS
ATTGGCTCAAATTGGGAAAA+GGG 0.425217 4_1:-51121783 Msa0553840:three_prime_UTR
GGCGAATCCAACGCCATGTT+CGG 0.426377 4_1:+51122092 None:intergenic
GAGTGAGGGTCGAGTGGTGA+AGG 0.427854 4_1:+51123166 None:intergenic
TGATGATATTGGCTCAAATT+GGG 0.430939 4_1:-51121790 Msa0553840:three_prime_UTR
AGTGATGAAGAAGAAGATAC+TGG 0.431284 4_1:+51123277 None:intergenic
GCTGTCGGCGGCGGACATTA+AGG 0.439203 4_1:+51123096 None:intergenic
ATCGGAGTGGCTGTCGGCGG+CGG 0.447860 4_1:+51123087 None:intergenic
TTTGGTGATGATGATGTTGT+TGG 0.448206 4_1:+51123365 None:intergenic
TGAAATCGATTTACTGAGAT+GGG 0.451988 4_1:+51122820 None:intergenic
CCAGTCGTCGGAGTCGAAAT+CGG 0.452148 4_1:+51123126 None:intergenic
AAACTCAGATAAACGATTAC+CGG 0.454956 4_1:+51122418 None:intergenic
ATCCGGTTGTGGTGAGAGTC+GGG 0.456439 4_1:+51123033 None:intergenic
TCACCGACCCATTTCAAGTC+GGG 0.456993 4_1:-51123225 Msa0553840:CDS
GATTTGGAGTGGTACGGTGG+CGG 0.464060 4_1:+51123000 None:intergenic
ATTTGGAGTGGTACGGTGGC+GGG 0.464653 4_1:+51123001 None:intergenic
GTAGCTGAAATCGATGAAAC+TGG 0.469167 4_1:+51122440 None:intergenic
GTGTGATTGGTGTGAGGGAG+AGG 0.470960 4_1:-51122022 Msa0553840:CDS
AATTTCTGGTATACCTGCTA+TGG 0.471549 4_1:-51122477 Msa0553840:CDS
GATTGGTGTGAGGGAGAGGA+TGG 0.472918 4_1:-51122018 Msa0553840:CDS
AATGTGCGAACTTTGAATAT+GGG 0.473696 4_1:+51122645 None:intergenic
GAATTTGCAGGTGGTGGGAA+TGG 0.473934 4_1:-51123482 Msa0553840:intron
GACCCATTTCAAGTCGGGTC+GGG 0.475045 4_1:-51123220 Msa0553840:CDS
AATTGGGATTTGGAGTGGTA+CGG 0.476385 4_1:+51122994 None:intergenic
TCTGTAAATTTGATTTGTAT+GGG 0.480060 4_1:-51121358 Msa0553840:three_prime_UTR
CGCTGACAGTGGAAACCTAA+TGG 0.480605 4_1:-51123449 Msa0553840:CDS
ATCAACTATATGAATATCGT+TGG 0.482770 4_1:+51122583 None:intergenic
GTGAAATCGATTTACTGAGA+TGG 0.489246 4_1:+51122819 None:intergenic
AGGTTATGGACTTGTAAAAT+GGG 0.491169 4_1:-51121430 Msa0553840:three_prime_UTR
ATCAATTGAAAACAAAAGAA+AGG 0.496213 4_1:+51121516 None:intergenic
CGGGTCGGGCATGATGAAAA+CGG 0.497777 4_1:+51123052 None:intergenic
GAAAACAAAAGAAAGGAAAA+GGG 0.498963 4_1:+51121523 None:intergenic
AAATTCTTGTTCTACTACAT+GGG 0.505141 4_1:-51122708 Msa0553840:CDS
AGGAGAATTGATGGTGTGAT+TGG 0.505945 4_1:-51122035 Msa0553840:CDS
GACCCGACCCGACTTGAAAT+GGG 0.507947 4_1:+51123218 None:intergenic
ATCGGAGAAGCGGAAGGTGG+TGG 0.509232 4_1:+51123144 None:intergenic
AAGCGGAAGGTGGTGGAGTG+AGG 0.511325 4_1:+51123151 None:intergenic
AAACGGGTTGAGATGGAAAT+CGG 0.511523 4_1:+51123069 None:intergenic
GGGTCGGGCATGATGAAAAC+GGG 0.514429 4_1:+51123053 None:intergenic
TGAGAGTTTGGAACCGAACA+TGG 0.520432 4_1:-51122105 Msa0553840:CDS
TTTGGAACCGAACATGGCGT+TGG 0.523106 4_1:-51122099 Msa0553840:CDS
GGTGAAGAAGCTATGCAGAT+TGG 0.523544 4_1:-51121656 Msa0553840:three_prime_UTR
CATCATCACCAAAACATCCT+AGG 0.524123 4_1:-51123355 Msa0553840:CDS
GGAAAAGGGTTTCTTCCACC+TGG 0.528044 4_1:-51121769 Msa0553840:three_prime_UTR
ATTAGGCAAAGCAGTGATCA+AGG 0.531199 4_1:-51121450 Msa0553840:three_prime_UTR
GGTTGAATTGGGATTTGGAG+TGG 0.535861 4_1:+51122989 None:intergenic
AATTGTTCAAGGGATTCAAT+GGG 0.536168 4_1:-51122555 Msa0553840:CDS
GTTGTGGTGAGAGTCGGGTC+GGG 0.536815 4_1:+51123038 None:intergenic
GTGGCGGGTAAGAGAAGATC+CGG 0.537694 4_1:+51123016 None:intergenic
TGAAGCTAGTTTGACGACTC+GGG 0.539102 4_1:-51122180 Msa0553840:CDS
AGCTCAATAGGTGTGAGAAT+CGG 0.541484 4_1:+51122362 None:intergenic
ATGGACTTGTAAAATGGGAA+TGG 0.542364 4_1:-51121425 Msa0553840:three_prime_UTR
TTTCGACTCCGACGACTGGA+TGG 0.543044 4_1:-51123122 Msa0553840:CDS
ATGAAGCTAGTTTGACGACT+CGG 0.543619 4_1:-51122181 Msa0553840:CDS
GATCCGGTTGTGGTGAGAGT+CGG 0.547170 4_1:+51123032 None:intergenic
GTTTCATCGATTTCAGCTAC+CGG 0.548117 4_1:-51122437 Msa0553840:CDS
CAAGAAGAAACAGTCAATAG+AGG 0.549819 4_1:+51121822 None:intergenic
AGTAAATCGATTTCACAAAA+CGG 0.553570 4_1:-51122812 Msa0553840:CDS
GAACATGGCGTTGGATTCGC+CGG 0.557782 4_1:-51122090 Msa0553840:CDS
GCTAGTTTGACGACTCGGGT+TGG 0.562442 4_1:-51122176 Msa0553840:CDS
CGACCCATTTCAAGTCGGGT+CGG 0.565104 4_1:-51123221 Msa0553840:CDS
TCTCTGTCTCACTACATCCA+TGG 0.569966 4_1:-51123318 Msa0553840:CDS
GTAGCATGTTGATGTTGCAC+TGG 0.570751 4_1:-51121474 Msa0553840:three_prime_UTR
AGGGAAGTTGAAAGCGGGAT+CGG 0.573814 4_1:+51123186 None:intergenic
GAAGTTCCTAAAGCCATAGC+AGG 0.575286 4_1:+51122464 None:intergenic
AGTGAGGGTCGAGTGGTGAA+GGG 0.576272 4_1:+51123167 None:intergenic
ATGGAAATCGGAGTGGCTGT+CGG 0.577416 4_1:+51123081 None:intergenic
GGATGGAGGATAAAGAACAG+TGG 0.578860 4_1:-51122001 Msa0553840:CDS
CTCTCCACCTGAAACCTCTC+CGG 0.581104 4_1:+51122071 None:intergenic
GGTTGTGGTGAGAGTCGGGT+CGG 0.584555 4_1:+51123037 None:intergenic
AGCGGAAGGTGGTGGAGTGA+GGG 0.584958 4_1:+51123152 None:intergenic
GTGGTGGAGTGAGGGTCGAG+TGG 0.585779 4_1:+51123160 None:intergenic
GGTGAAGGGAAGTTGAAAGC+GGG 0.589096 4_1:+51123181 None:intergenic
ACCTTCAAAATTCTAACATG+TGG 0.590768 4_1:+51124704 None:intergenic
AGAGAGGGTTTGTGATACCT+AGG 0.592419 4_1:+51123338 None:intergenic
ATGATGAAAACGGGTTGAGA+TGG 0.594967 4_1:+51123062 None:intergenic
GGAGGATAAAGAACAGTGGA+AGG 0.599058 4_1:-51121997 Msa0553840:CDS
TGGTGTGAGGGAGAGGATGG+AGG 0.600379 4_1:-51122015 Msa0553840:CDS
GGATGTAGTGAGACAGAGAG+AGG 0.601119 4_1:+51123322 None:intergenic
GCAATTCTTGAAGCAACAGA+AGG 0.601796 4_1:-51122608 Msa0553840:CDS
TTCACCGACCCATTTCAAGT+CGG 0.604924 4_1:-51123226 Msa0553840:CDS
TGATGGTGTGATTGGTGTGA+GGG 0.605734 4_1:-51122027 Msa0553840:CDS
TGGGATTTGGAGTGGTACGG+TGG 0.612286 4_1:+51122997 None:intergenic
GTCAAGCTAAGATATTGTTG+TGG 0.613839 4_1:-51121920 Msa0553840:CDS
TAAGGTATCCATCCAGTCGT+CGG 0.619410 4_1:+51123114 None:intergenic
AAAGAAAGGAAAAGGGTGTG+TGG 0.623975 4_1:+51121530 None:intergenic
AATGGGTCGGTGAAATCGGT+AGG 0.625917 4_1:+51123235 None:intergenic
TTCGCCGGAGAGGTTTCAGG+TGG 0.626303 4_1:-51122075 Msa0553840:CDS
GGTAAGAGAAGATCCGGTTG+TGG 0.627421 4_1:+51123022 None:intergenic
CGGAGTCGAAATCGGAGAAG+CGG 0.634008 4_1:+51123134 None:intergenic
GAAATCGGAGTGGCTGTCGG+CGG 0.636240 4_1:+51123084 None:intergenic
GTCGAAATCGGAGAAGCGGA+AGG 0.636626 4_1:+51123138 None:intergenic
GATGTAGTGAGACAGAGAGA+GGG 0.639502 4_1:+51123323 None:intergenic
GGGTCGGTGAAATCGGTAGG+TGG 0.649301 4_1:+51123238 None:intergenic
GCTTACATGTGCGCTGACAG+TGG 0.651171 4_1:-51123460 Msa0553840:CDS
TGGCGTTGGATTCGCCGGAG+AGG 0.656610 4_1:-51122085 Msa0553840:CDS
TTGATGGTGTGATTGGTGTG+AGG 0.659575 4_1:-51122028 Msa0553840:CDS
TGGTGAAGGGAAGTTGAAAG+CGG 0.662739 4_1:+51123180 None:intergenic
GAAATCGGAGAAGCGGAAGG+TGG 0.667417 4_1:+51123141 None:intergenic
CGACCCGACTTGAAATGGGT+CGG 0.673110 4_1:+51123222 None:intergenic
TCGGTAGGTGGAAAAGACAA+AGG 0.681946 4_1:+51123250 None:intergenic
AAACGACGATGTCGTTTCGG+TGG 0.687537 4_1:+51122934 None:intergenic
GGTTGAGATGGAAATCGGAG+TGG 0.716005 4_1:+51123074 None:intergenic
GTAGGTGGAAAAGACAAAGG+TGG 0.720227 4_1:+51123253 None:intergenic
AGAAGCTATTGAAGATTGTG+CGG 0.726727 4_1:+51122733 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGCATTTTTTATTTTTTTTT+TGG + chr4_1:51122089-51122108 None:intergenic 10.0%
!! TAATTAAATTAATTATCGTT+TGG - chr4_1:51124442-51124461 Msa0553840:intron 10.0%
!! AATTAAATTAATTATCGTTT+GGG - chr4_1:51124443-51124462 Msa0553840:intron 10.0%
!!! ATGAAAATGTCTTTTAAAAT+GGG + chr4_1:51122221-51122240 None:intergenic 15.0%
!!! AATGAAAATGTCTTTTAAAA+TGG + chr4_1:51122222-51122241 None:intergenic 15.0%
!!! TGATTTTCCTTTTTTTTTTT+TGG - chr4_1:51124527-51124546 Msa0553840:intron 15.0%
!!! GATTTTCCTTTTTTTTTTTT+GGG - chr4_1:51124528-51124547 Msa0553840:intron 15.0%
!! TATTTGTCTAAATATCAAAA+AGG - chr4_1:51124695-51124714 Msa0553840:intron 15.0%
!! TTTATATGATGTTGTATTGT+GGG - chr4_1:51121601-51121620 Msa0553840:three_prime_UTR 20.0%
!! TTAGTTAAAGACAAACAAAT+AGG - chr4_1:51121769-51121788 Msa0553840:three_prime_UTR 20.0%
!!! TATACCGTCTTATATTTTTT+TGG - chr4_1:51121850-51121869 Msa0553840:CDS 20.0%
!!! ATACCGTCTTATATTTTTTT+GGG - chr4_1:51121851-51121870 Msa0553840:CDS 20.0%
!! TAACCCAAAAAAATATAAGA+CGG + chr4_1:51121857-51121876 None:intergenic 20.0%
!! AAAATAAAAATGTAAGAGAG+TGG - chr4_1:51122168-51122187 Msa0553840:CDS 20.0%
!! AAATAAAAATGTAAGAGAGT+GGG - chr4_1:51122169-51122188 Msa0553840:CDS 20.0%
!!! GATATTCATATAGTTGATTT+TGG - chr4_1:51123446-51123465 Msa0553840:CDS 20.0%
!! ATCAATTGAAAACAAAAGAA+AGG + chr4_1:51124511-51124530 None:intergenic 20.0%
!!! TCTGTAAATTTGATTTGTAT+GGG - chr4_1:51124666-51124685 Msa0553840:intron 20.0%
! CAGATACAAATGTAATATAG+AGG - chr4_1:51121448-51121467 Msa0553840:three_prime_UTR 25.0%
!!! TCTCAATCTCAATTTTGTTT+CGG + chr4_1:51121490-51121509 None:intergenic 25.0%
! GTTTATATGATGTTGTATTG+TGG - chr4_1:51121600-51121619 Msa0553840:three_prime_UTR 25.0%
! CAAAATCCTTATTCTCAAAT+TGG + chr4_1:51121637-51121656 None:intergenic 25.0%
! TTATAATCGTTAAAGTGTGA+GGG + chr4_1:51121797-51121816 None:intergenic 25.0%
! ATTATAATCGTTAAAGTGTG+AGG + chr4_1:51121798-51121817 None:intergenic 25.0%
! CATTATAAGAAACTTTGTCT+CGG - chr4_1:51121953-51121972 Msa0553840:CDS 25.0%
!! AATGTTAACCATTGTCTTTT+GGG + chr4_1:51122064-51122083 None:intergenic 25.0%
!! AAATGTTAACCATTGTCTTT+TGG + chr4_1:51122065-51122084 None:intergenic 25.0%
!!! ACTGAATATTTTGAATTTGC+AGG - chr4_1:51122530-51122549 Msa0553840:CDS 25.0%
!!! AATTGCTTGATTTGGTTTTT+GGG + chr4_1:51123174-51123193 None:intergenic 25.0%
!! AGTAAATCGATTTCACAAAA+CGG - chr4_1:51123212-51123231 Msa0553840:CDS 25.0%
! AAAAGGAAACTCTTTGATTA+GGG + chr4_1:51123241-51123260 None:intergenic 25.0%
! TAAAAGGAAACTCTTTGATT+AGG + chr4_1:51123242-51123261 None:intergenic 25.0%
! AAATTCTTGTTCTACTACAT+GGG - chr4_1:51123316-51123335 Msa0553840:CDS 25.0%
! ATCAACTATATGAATATCGT+TGG + chr4_1:51123444-51123463 None:intergenic 25.0%
!!! TTGATTTTGGAATTGTTCAA+GGG - chr4_1:51123459-51123478 Msa0553840:CDS 25.0%
!!! AATCCTAAGATTGTTACTTT+AGG - chr4_1:51123815-51123834 Msa0553840:intron 25.0%
!! TCAATTCATAATGTTTTGCT+TGG - chr4_1:51124347-51124366 Msa0553840:intron 25.0%
! GAAAACAAAAGAAAGGAAAA+GGG + chr4_1:51124504-51124523 None:intergenic 25.0%
! TGAAAACAAAAGAAAGGAAA+AGG + chr4_1:51124505-51124524 None:intergenic 25.0%
! ATGCTACCCAAAAAAAAAAA+AGG + chr4_1:51124537-51124556 None:intergenic 25.0%
!!! CTCTGTAAATTTGATTTGTA+TGG - chr4_1:51124665-51124684 Msa0553840:intron 25.0%
ACCTTCAAAATTCTAACATG+TGG + chr4_1:51121323-51121342 None:intergenic 30.0%
CTATAAACAACATCTCTCTT+TGG + chr4_1:51121407-51121426 None:intergenic 30.0%
! AGAGAGATGTTGTTTATAGA+TGG - chr4_1:51121408-51121427 Msa0553840:three_prime_UTR 30.0%
TTACATTTGTATCTGCCTTT+GGG + chr4_1:51121443-51121462 None:intergenic 30.0%
ATTACATTTGTATCTGCCTT+TGG + chr4_1:51121444-51121463 None:intergenic 30.0%
!! TCAATCTCAATTTTGTTTCG+GGG + chr4_1:51121488-51121507 None:intergenic 30.0%
!! CTCAATCTCAATTTTGTTTC+GGG + chr4_1:51121489-51121508 None:intergenic 30.0%
ATATGTATTCGCAGTAACAA+TGG - chr4_1:51121509-51121528 Msa0553840:three_prime_UTR 30.0%
TGATCTCCAATTTGAGAATA+AGG - chr4_1:51121628-51121647 Msa0553840:three_prime_UTR 30.0%
TTGTCTATACTCAAAGAAAG+CGG - chr4_1:51121654-51121673 Msa0553840:three_prime_UTR 30.0%
! AAACAAAGTGCTTCCAAATT+CGG + chr4_1:51121712-51121731 None:intergenic 30.0%
CGAGACAAAGTTTCTTATAA+TGG + chr4_1:51121955-51121974 None:intergenic 30.0%
AGAAAGTTTGCATCAAAAAG+TGG + chr4_1:51122018-51122037 None:intergenic 30.0%
! ATGTTAACCATTGTCTTTTG+GGG + chr4_1:51122063-51122082 None:intergenic 30.0%
TTATAGTCGTTCAATGTTCT+AGG - chr4_1:51122242-51122261 Msa0553840:CDS 30.0%
!! ATGGTGTAATAGATTGGTTT+GGG + chr4_1:51122424-51122443 None:intergenic 30.0%
!! ATGATGATGGTGTAATAGAT+TGG + chr4_1:51122430-51122449 None:intergenic 30.0%
!! GAATATTTTGAATTTGCAGG+TGG - chr4_1:51122533-51122552 Msa0553840:CDS 30.0%
!!! GTTGTTCTTGTTGTTGTTTT+TGG + chr4_1:51122623-51122642 None:intergenic 30.0%
TAAAGAAGCGATTTCTGTTA+GGG + chr4_1:51123144-51123163 None:intergenic 30.0%
TTAAAGAAGCGATTTCTGTT+AGG + chr4_1:51123145-51123164 None:intergenic 30.0%
!!! CAATTGCTTGATTTGGTTTT+TGG + chr4_1:51123175-51123194 None:intergenic 30.0%
! TGAAATCGATTTACTGAGAT+GGG + chr4_1:51123207-51123226 None:intergenic 30.0%
CAAATTCTTGTTCTACTACA+TGG - chr4_1:51123315-51123334 Msa0553840:CDS 30.0%
AATGTGCGAACTTTGAATAT+GGG + chr4_1:51123382-51123401 None:intergenic 30.0%
AAATGTGCGAACTTTGAATA+TGG + chr4_1:51123383-51123402 None:intergenic 30.0%
!!! GTTGATTTTGGAATTGTTCA+AGG - chr4_1:51123458-51123477 Msa0553840:CDS 30.0%
AATTGTTCAAGGGATTCAAT+GGG - chr4_1:51123469-51123488 Msa0553840:CDS 30.0%
CCAGAAATTCTTACACTATT+AGG + chr4_1:51123536-51123555 None:intergenic 30.0%
CCTAATAGTGTAAGAATTTC+TGG - chr4_1:51123533-51123552 Msa0553840:intron 30.0%
AAACTCAGATAAACGATTAC+CGG + chr4_1:51123609-51123628 None:intergenic 30.0%
! CTTTTGTATTCAACCAAATG+AGG - chr4_1:51123700-51123719 Msa0553840:intron 30.0%
! GCTTCAGTTGTATAATCTTT+TGG - chr4_1:51123742-51123761 Msa0553840:intron 30.0%
TCACCTAAAGTAACAATCTT+AGG + chr4_1:51123821-51123840 None:intergenic 30.0%
TAGTTCTTTGTATTCTCTTG+TGG - chr4_1:51124138-51124157 Msa0553840:intron 30.0%
!! TTGATGATATTGGCTCAAAT+TGG - chr4_1:51124233-51124252 Msa0553840:intron 30.0%
!! TGATGATATTGGCTCAAATT+GGG - chr4_1:51124234-51124253 Msa0553840:intron 30.0%
! ACTCTGCAATACAAGATTTT+TGG - chr4_1:51124413-51124432 Msa0553840:intron 30.0%
! CTCTGCAATACAAGATTTTT+GGG - chr4_1:51124414-51124433 Msa0553840:intron 30.0%
AAGGTTATGGACTTGTAAAA+TGG - chr4_1:51124593-51124612 Msa0553840:intron 30.0%
AGGTTATGGACTTGTAAAAT+GGG - chr4_1:51124594-51124613 Msa0553840:intron 30.0%
! GCCACATGTTAGAATTTTGA+AGG - chr4_1:51121319-51121338 Msa0553840:three_prime_UTR 35.0%
CATCTCTCTTTGGTTATTGT+TGG + chr4_1:51121397-51121416 None:intergenic 35.0%
!! CAATCTCAATTTTGTTTCGG+GGG + chr4_1:51121487-51121506 None:intergenic 35.0%
TCAACTTACATCGAACACAA+TGG + chr4_1:51121569-51121588 None:intergenic 35.0%
ATTGTGTTCGATGTAAGTTG+AGG - chr4_1:51121568-51121587 Msa0553840:three_prime_UTR 35.0%
CAACAATGACATACCGAATT+TGG - chr4_1:51121696-51121715 Msa0553840:three_prime_UTR 35.0%
ATCTTTAACCATTGCCCTTA+GGG + chr4_1:51121931-51121950 None:intergenic 35.0%
AATCTTTAACCATTGCCCTT+AGG + chr4_1:51121932-51121951 None:intergenic 35.0%
CTAACTTGAGTTCGATTCTT+GGG + chr4_1:51122115-51122134 None:intergenic 35.0%
ACTAACTTGAGTTCGATTCT+TGG + chr4_1:51122116-51122135 None:intergenic 35.0%
AAATCACACACACACTTCAA+CGG - chr4_1:51122316-51122335 Msa0553840:CDS 35.0%
GAAGTAGAAAAGAGTGAGTA+AGG + chr4_1:51122370-51122389 None:intergenic 35.0%
! GATGGTGTAATAGATTGGTT+TGG + chr4_1:51122425-51122444 None:intergenic 35.0%
!! GTTATGGTGATTGATGATGA+TGG + chr4_1:51122443-51122462 None:intergenic 35.0%
TCATCATCAATCACCATAAC+AGG - chr4_1:51122443-51122462 Msa0553840:CDS 35.0%
!! TATTTTGAATTTGCAGGTGG+TGG - chr4_1:51122536-51122555 Msa0553840:CDS 35.0%
! ATTTTGAATTTGCAGGTGGT+GGG - chr4_1:51122537-51122556 Msa0553840:CDS 35.0%
!! TTTGGTGATGATGATGTTGT+TGG + chr4_1:51122662-51122681 None:intergenic 35.0%
! TTGTGATACCTAGGATGTTT+TGG + chr4_1:51122680-51122699 None:intergenic 35.0%
AGTGATGAAGAAGAAGATAC+TGG + chr4_1:51122750-51122769 None:intergenic 35.0%
!!! CTGTTAGGGTTTTAAGAAGT+GGG + chr4_1:51123130-51123149 None:intergenic 35.0%
!!! TCTGTTAGGGTTTTAAGAAG+TGG + chr4_1:51123131-51123150 None:intergenic 35.0%
AGGGTTTCAATTGCTTGATT+TGG + chr4_1:51123182-51123201 None:intergenic 35.0%
! GTGAAATCGATTTACTGAGA+TGG + chr4_1:51123208-51123227 None:intergenic 35.0%
TAGGGCTTGTGAAAAGTAAA+AGG + chr4_1:51123258-51123277 None:intergenic 35.0%
AGAAGCTATTGAAGATTGTG+CGG + chr4_1:51123294-51123313 None:intergenic 35.0%
GAATTGTTCAAGGGATTCAA+TGG - chr4_1:51123468-51123487 Msa0553840:CDS 35.0%
!! AATTTCTGGTATACCTGCTA+TGG - chr4_1:51123547-51123566 Msa0553840:intron 35.0%
GATTCATCAAGAAGCTCAAT+AGG + chr4_1:51123677-51123696 None:intergenic 35.0%
TTAACAGCTAAAGCCTCATT+TGG + chr4_1:51123716-51123735 None:intergenic 35.0%
!!! TGCTGCTTTTTTTGAGAGTT+TGG - chr4_1:51123907-51123926 Msa0553840:intron 35.0%
GTCAAGCTAAGATATTGTTG+TGG - chr4_1:51124104-51124123 Msa0553840:intron 35.0%
CAAGAAGAAACAGTCAATAG+AGG + chr4_1:51124205-51124224 None:intergenic 35.0%
! CTCTATTGACTGTTTCTTCT+TGG - chr4_1:51124203-51124222 Msa0553840:intron 35.0%
!! TGGCGTTGATTTGATGATAT+TGG - chr4_1:51124223-51124242 Msa0553840:intron 35.0%
!! TATTGGCTCAAATTGGGAAA+AGG - chr4_1:51124240-51124259 Msa0553840:intron 35.0%
! ATTGGCTCAAATTGGGAAAA+GGG - chr4_1:51124241-51124260 Msa0553840:intron 35.0%
ACACCAGAGAAAAAAAAACC+AGG + chr4_1:51124276-51124295 None:intergenic 35.0%
!!! TGGTTTTTTTTTCTCTGGTG+TGG - chr4_1:51124275-51124294 Msa0553840:intron 35.0%
CTATGCAGATTGGATATGTT+TGG - chr4_1:51124378-51124397 Msa0553840:intron 35.0%
!!! GGGTTGTATTTTTAGCAGTT+TGG - chr4_1:51124463-51124482 Msa0553840:intron 35.0%
ATGGACTTGTAAAATGGGAA+TGG - chr4_1:51124599-51124618 Msa0553840:intron 35.0%
! AAGGTTAGCCAACGTAAACA+GGG - chr4_1:51121338-51121357 Msa0553840:three_prime_UTR 40.0%
TCTATACTCAAAGAAAGCGG+TGG - chr4_1:51121657-51121676 Msa0553840:three_prime_UTR 40.0%
GAATGCGACTTAGCTAAAGA+TGG + chr4_1:51121821-51121840 None:intergenic 40.0%
AAAATGTAAGAGAGTGGGTG+TGG - chr4_1:51122174-51122193 Msa0553840:CDS 40.0%
AAATGTAAGAGAGTGGGTGT+GGG - chr4_1:51122175-51122194 Msa0553840:CDS 40.0%
GTTGAAGTGTGTGTGTGATT+TGG + chr4_1:51122317-51122336 None:intergenic 40.0%
!! GTAATAGATTGGTTTGGGTG+TGG + chr4_1:51122419-51122438 None:intergenic 40.0%
CTTGTTGAGCTATTGCCATT+AGG + chr4_1:51122593-51122612 None:intergenic 40.0%
CATCATCACCAAAACATCCT+AGG - chr4_1:51122669-51122688 Msa0553840:CDS 40.0%
! ATGTTGTTGTTGTTGTGCCA+TGG + chr4_1:51122726-51122745 None:intergenic 40.0%
TTGAAATGGGTCGGTGAAAT+CGG + chr4_1:51122796-51122815 None:intergenic 40.0%
!! AAACGGGTTGAGATGGAAAT+CGG + chr4_1:51122958-51122977 None:intergenic 40.0%
!! ATGATGAAAACGGGTTGAGA+TGG + chr4_1:51122965-51122984 None:intergenic 40.0%
! AATTGGGATTTGGAGTGGTA+CGG + chr4_1:51123033-51123052 None:intergenic 40.0%
!!! TTAGTGTCGTTTTTGCGTTG+TGG + chr4_1:51123068-51123087 None:intergenic 40.0%
! TGAAAACGACGATGTCGTTT+CGG + chr4_1:51123096-51123115 None:intergenic 40.0%
!!! GGTTTTAAGAAGTGGGTTTG+TGG + chr4_1:51123123-51123142 None:intergenic 40.0%
!! CGATTTACTGAGATGGGTTA+GGG + chr4_1:51123201-51123220 None:intergenic 40.0%
! TCGATTTACTGAGATGGGTT+AGG + chr4_1:51123202-51123221 None:intergenic 40.0%
!! TGCGGTGATTTTGTTGGTTA+GGG + chr4_1:51123276-51123295 None:intergenic 40.0%
! AGATTGTGCGGTGATTTTGT+TGG + chr4_1:51123282-51123301 None:intergenic 40.0%
GCAATTCTTGAAGCAACAGA+AGG - chr4_1:51123416-51123435 Msa0553840:CDS 40.0%
GTAGCTGAAATCGATGAAAC+TGG + chr4_1:51123587-51123606 None:intergenic 40.0%
!! GTTTCATCGATTTCAGCTAC+CGG - chr4_1:51123587-51123606 Msa0553840:intron 40.0%
AGCTCAATAGGTGTGAGAAT+CGG + chr4_1:51123665-51123684 None:intergenic 40.0%
!!! CAAACGAAGCGCTTTTTCAA+CGG + chr4_1:51123783-51123802 None:intergenic 40.0%
! ATGAAGCTAGTTTGACGACT+CGG - chr4_1:51123843-51123862 Msa0553840:intron 40.0%
TTGCTTGGTAGGAGAATTGA+TGG - chr4_1:51123980-51123999 Msa0553840:intron 40.0%
!! AGGAGAATTGATGGTGTGAT+TGG - chr4_1:51123989-51124008 Msa0553840:intron 40.0%
!! AGAACAGTGGAAGGTTTTGA+TGG - chr4_1:51124036-51124055 Msa0553840:intron 40.0%
!! AAGGTTTTGATGGAAAGCTG+TGG - chr4_1:51124046-51124065 Msa0553840:intron 40.0%
!!! AAGCTGTGGTTTTGAATCAG+TGG - chr4_1:51124060-51124079 Msa0553840:intron 40.0%
!!! AGCTGTGGTTTTGAATCAGT+GGG - chr4_1:51124061-51124080 Msa0553840:intron 40.0%
!!! TCAACCTGCTTTTTTGTCTC+TGG - chr4_1:51124165-51124184 Msa0553840:intron 40.0%
CCAGAGAAAAAAAAACCAGG+TGG + chr4_1:51124273-51124292 None:intergenic 40.0%
!!! CCACCTGGTTTTTTTTTCTC+TGG - chr4_1:51124270-51124289 Msa0553840:intron 40.0%
! AAAGAAAGGAAAAGGGTGTG+TGG + chr4_1:51124497-51124516 None:intergenic 40.0%
! GTTGATGTTGCACTGGTATT+AGG - chr4_1:51124557-51124576 Msa0553840:intron 40.0%
ATTAGGCAAAGCAGTGATCA+AGG - chr4_1:51124574-51124593 Msa0553840:intron 40.0%
CAAAGCAGTGATCAAGGTTA+TGG - chr4_1:51124580-51124599 Msa0553840:intron 40.0%
! GAAGGTTAGCCAACGTAAAC+AGG - chr4_1:51121337-51121356 Msa0553840:three_prime_UTR 45.0%
! AGGTTAGCCAACGTAAACAG+GGG - chr4_1:51121339-51121358 Msa0553840:three_prime_UTR 45.0%
GTTTCGGGGGAAAGAAAAAG+AGG + chr4_1:51121474-51121493 None:intergenic 45.0%
TCTTGCTAACCAATGCCCTA+AGG - chr4_1:51121913-51121932 Msa0553840:CDS 45.0%
CTTGCTAACCAATGCCCTAA+GGG - chr4_1:51121914-51121933 Msa0553840:CDS 45.0%
ACCAATGCCCCAAAAGACAA+TGG - chr4_1:51122053-51122072 Msa0553840:CDS 45.0%
!!! ACCATTGTCTTTTGGGGCAT+TGG + chr4_1:51122057-51122076 None:intergenic 45.0%
AAGGAAACACTCACTCACTC+GGG + chr4_1:51122276-51122295 None:intergenic 45.0%
CAAGGAAACACTCACTCACT+CGG + chr4_1:51122277-51122296 None:intergenic 45.0%
! ATTTGGAGTGGAGAGAAGAG+AGG + chr4_1:51122300-51122319 None:intergenic 45.0%
! AGTGTGTGTGTGATTTGGAG+TGG + chr4_1:51122312-51122331 None:intergenic 45.0%
AGTGAGTAAGGTGAGAGAGT+GGG + chr4_1:51122358-51122377 None:intergenic 45.0%
AGAGAGAGAGGAACCTGTTA+TGG + chr4_1:51122459-51122478 None:intergenic 45.0%
GATGTATGAGAGAGAGAGAG+AGG + chr4_1:51122471-51122490 None:intergenic 45.0%
AGAGAGGGTTTGTGATACCT+AGG + chr4_1:51122689-51122708 None:intergenic 45.0%
GATGTAGTGAGACAGAGAGA+GGG + chr4_1:51122704-51122723 None:intergenic 45.0%
TCTCTGTCTCACTACATCCA+TGG - chr4_1:51122706-51122725 Msa0553840:CDS 45.0%
GTAGGTGGAAAAGACAAAGG+TGG + chr4_1:51122774-51122793 None:intergenic 45.0%
TCGGTAGGTGGAAAAGACAA+AGG + chr4_1:51122777-51122796 None:intergenic 45.0%
! TTCACCGACCCATTTCAAGT+CGG - chr4_1:51122798-51122817 Msa0553840:CDS 45.0%
TGGTGAAGGGAAGTTGAAAG+CGG + chr4_1:51122847-51122866 None:intergenic 45.0%
! TAAGGTATCCATCCAGTCGT+CGG + chr4_1:51122913-51122932 None:intergenic 45.0%
GGTTGAATTGGGATTTGGAG+TGG + chr4_1:51123038-51123057 None:intergenic 45.0%
TGGGTGGTTGAATTGGGATT+TGG + chr4_1:51123043-51123062 None:intergenic 45.0%
!! TCGTTTTTGCGTTGTGGAGT+GGG + chr4_1:51123062-51123081 None:intergenic 45.0%
!! GTGCGGTGATTTTGTTGGTT+AGG + chr4_1:51123277-51123296 None:intergenic 45.0%
GCATTTGCAACTCGTTCTTC+TGG - chr4_1:51123506-51123525 Msa0553840:intron 45.0%
GGTATACCTGCTATGGCTTT+AGG - chr4_1:51123554-51123573 Msa0553840:intron 45.0%
GAAGTTCCTAAAGCCATAGC+AGG + chr4_1:51123563-51123582 None:intergenic 45.0%
!! CGTTGAAAAAGCGCTTCGTT+TGG - chr4_1:51123781-51123800 Msa0553840:intron 45.0%
! TGAAGCTAGTTTGACGACTC+GGG - chr4_1:51123844-51123863 Msa0553840:intron 45.0%
! TGAGAGTTTGGAACCGAACA+TGG - chr4_1:51123919-51123938 Msa0553840:intron 45.0%
! TGGAGAGTCTTTTGCTTGGT+AGG - chr4_1:51123969-51123988 Msa0553840:intron 45.0%
!! TTGATGGTGTGATTGGTGTG+AGG - chr4_1:51123996-51124015 Msa0553840:intron 45.0%
! TGATGGTGTGATTGGTGTGA+GGG - chr4_1:51123997-51124016 Msa0553840:intron 45.0%
GGATGGAGGATAAAGAACAG+TGG - chr4_1:51124023-51124042 Msa0553840:intron 45.0%
GGAGGATAAAGAACAGTGGA+AGG - chr4_1:51124027-51124046 Msa0553840:intron 45.0%
CAAGCCAGAGACAAAAAAGC+AGG + chr4_1:51124172-51124191 None:intergenic 45.0%
!!! CTGCTTTTTTGTCTCTGGCT+TGG - chr4_1:51124170-51124189 Msa0553840:intron 45.0%
GGTGAAGAAGCTATGCAGAT+TGG - chr4_1:51124368-51124387 Msa0553840:intron 45.0%
GTAGCATGTTGATGTTGCAC+TGG - chr4_1:51124550-51124569 Msa0553840:intron 45.0%
! GGTTAGCCAACGTAAACAGG+GGG - chr4_1:51121340-51121359 Msa0553840:three_prime_UTR 50.0%
TTGTCCCGTCATGGGTAGAA+CGG - chr4_1:51121370-51121389 Msa0553840:three_prime_UTR 50.0%
TTGGACCGTTCTACCCATGA+CGG + chr4_1:51121378-51121397 None:intergenic 50.0%
AACCAATGCCCTAAGGGCAA+TGG - chr4_1:51121920-51121939 Msa0553840:CDS 50.0%
AACCATTGCCCTTAGGGCAT+TGG + chr4_1:51121925-51121944 None:intergenic 50.0%
AAACACTCACTCACTCGGGA+GGG + chr4_1:51122272-51122291 None:intergenic 50.0%
GAGTGAGTAAGGTGAGAGAG+TGG + chr4_1:51122359-51122378 None:intergenic 50.0%
!! GAATTTGCAGGTGGTGGGAA+TGG - chr4_1:51122542-51122561 Msa0553840:CDS 50.0%
CGCTGACAGTGGAAACCTAA+TGG - chr4_1:51122575-51122594 Msa0553840:CDS 50.0%
GGATGTAGTGAGACAGAGAG+AGG + chr4_1:51122705-51122724 None:intergenic 50.0%
AATGGGTCGGTGAAATCGGT+AGG + chr4_1:51122792-51122811 None:intergenic 50.0%
! TCACCGACCCATTTCAAGTC+GGG - chr4_1:51122799-51122818 Msa0553840:CDS 50.0%
!! AAAGCGGGATCGGTGTTTTC+AGG + chr4_1:51122831-51122850 None:intergenic 50.0%
AGGGAAGTTGAAAGCGGGAT+CGG + chr4_1:51122841-51122860 None:intergenic 50.0%
GGTGAAGGGAAGTTGAAAGC+GGG + chr4_1:51122846-51122865 None:intergenic 50.0%
ATGGAAATCGGAGTGGCTGT+CGG + chr4_1:51122946-51122965 None:intergenic 50.0%
GGTTGAGATGGAAATCGGAG+TGG + chr4_1:51122953-51122972 None:intergenic 50.0%
GGTAAGAGAAGATCCGGTTG+TGG + chr4_1:51123005-51123024 None:intergenic 50.0%
GTGGAGTGGGTGGTTGAATT+GGG + chr4_1:51123049-51123068 None:intergenic 50.0%
TGTGGAGTGGGTGGTTGAAT+TGG + chr4_1:51123050-51123069 None:intergenic 50.0%
! TTTTTGCGTTGTGGAGTGGG+TGG + chr4_1:51123059-51123078 None:intergenic 50.0%
!!! GTCGTTTTTGCGTTGTGGAG+TGG + chr4_1:51123063-51123082 None:intergenic 50.0%
! AAACGACGATGTCGTTTCGG+TGG + chr4_1:51123093-51123112 None:intergenic 50.0%
! TTTGGAACCGAACATGGCGT+TGG - chr4_1:51123925-51123944 Msa0553840:intron 50.0%
! CAGGTGGAGAGTCTTTTGCT+TGG - chr4_1:51123965-51123984 Msa0553840:intron 50.0%
GGAAAAGGGTTTCTTCCACC+TGG - chr4_1:51124255-51124274 Msa0553840:intron 50.0%
ACTCGACCCCCTGTTTACGT+TGG + chr4_1:51121349-51121368 None:intergenic 55.0%
! GTCGAGTGTTGTCCCGTCAT+GGG - chr4_1:51121362-51121381 Msa0553840:three_prime_UTR 55.0%
TGGACCGTTCTACCCATGAC+GGG + chr4_1:51121377-51121396 None:intergenic 55.0%
AGATGGCGAAACGAGCCCAA+AGG - chr4_1:51121425-51121444 Msa0553840:three_prime_UTR 55.0%
GAAACACTCACTCACTCGGG+AGG + chr4_1:51122273-51122292 None:intergenic 55.0%
GAGTGGAGAGAAGAGAGGCA+AGG + chr4_1:51122295-51122314 None:intergenic 55.0%
! GCTTACATGTGCGCTGACAG+TGG - chr4_1:51122564-51122583 Msa0553840:CDS 55.0%
! CGACCCGACTTGAAATGGGT+CGG + chr4_1:51122805-51122824 None:intergenic 55.0%
! CGACCCATTTCAAGTCGGGT+CGG - chr4_1:51122803-51122822 Msa0553840:CDS 55.0%
! GACCCATTTCAAGTCGGGTC+GGG - chr4_1:51122804-51122823 Msa0553840:CDS 55.0%
! GACCCGACCCGACTTGAAAT+GGG + chr4_1:51122809-51122828 None:intergenic 55.0%
AGTGAGGGTCGAGTGGTGAA+GGG + chr4_1:51122860-51122879 None:intergenic 55.0%
GAAATCGGAGAAGCGGAAGG+TGG + chr4_1:51122886-51122905 None:intergenic 55.0%
GTCGAAATCGGAGAAGCGGA+AGG + chr4_1:51122889-51122908 None:intergenic 55.0%
CGGAGTCGAAATCGGAGAAG+CGG + chr4_1:51122893-51122912 None:intergenic 55.0%
CCAGTCGTCGGAGTCGAAAT+CGG + chr4_1:51122901-51122920 None:intergenic 55.0%
TTTCGACTCCGACGACTGGA+TGG - chr4_1:51122902-51122921 Msa0553840:CDS 55.0%
GGGTCGGGCATGATGAAAAC+GGG + chr4_1:51122974-51122993 None:intergenic 55.0%
CGGGTCGGGCATGATGAAAA+CGG + chr4_1:51122975-51122994 None:intergenic 55.0%
ATCCGGTTGTGGTGAGAGTC+GGG + chr4_1:51122994-51123013 None:intergenic 55.0%
GATCCGGTTGTGGTGAGAGT+CGG + chr4_1:51122995-51123014 None:intergenic 55.0%
!! GTGGCGGGTAAGAGAAGATC+CGG + chr4_1:51123011-51123030 None:intergenic 55.0%
! ATTTGGAGTGGTACGGTGGC+GGG + chr4_1:51123026-51123045 None:intergenic 55.0%
! GATTTGGAGTGGTACGGTGG+CGG + chr4_1:51123027-51123046 None:intergenic 55.0%
! TGGGATTTGGAGTGGTACGG+TGG + chr4_1:51123030-51123049 None:intergenic 55.0%
! GCTAGTTTGACGACTCGGGT+TGG - chr4_1:51123848-51123867 Msa0553840:intron 55.0%
GGCGAATCCAACGCCATGTT+CGG + chr4_1:51123935-51123954 None:intergenic 55.0%
! GAACATGGCGTTGGATTCGC+CGG - chr4_1:51123934-51123953 Msa0553840:intron 55.0%
CTCTCCACCTGAAACCTCTC+CGG + chr4_1:51123956-51123975 None:intergenic 55.0%
GTGTGATTGGTGTGAGGGAG+AGG - chr4_1:51124002-51124021 Msa0553840:intron 55.0%
GATTGGTGTGAGGGAGAGGA+TGG - chr4_1:51124006-51124025 Msa0553840:intron 55.0%
! GGTCGAGTGTTGTCCCGTCA+TGG - chr4_1:51121361-51121380 Msa0553840:three_prime_UTR 60.0%
! TGGGACCAAGAGCACGAGGA+TGG - chr4_1:51122194-51122213 Msa0553840:CDS 60.0%
TGGGACCATCCTCGTGCTCT+TGG + chr4_1:51122202-51122221 None:intergenic 60.0%
GGGTCGGTGAAATCGGTAGG+TGG + chr4_1:51122789-51122808 None:intergenic 60.0%
! GGACCCGACCCGACTTGAAA+TGG + chr4_1:51122810-51122829 None:intergenic 60.0%
GAGTGAGGGTCGAGTGGTGA+AGG + chr4_1:51122861-51122880 None:intergenic 60.0%
!! AGCGGAAGGTGGTGGAGTGA+GGG + chr4_1:51122875-51122894 None:intergenic 60.0%
!! AAGCGGAAGGTGGTGGAGTG+AGG + chr4_1:51122876-51122895 None:intergenic 60.0%
ATCGGAGAAGCGGAAGGTGG+TGG + chr4_1:51122883-51122902 None:intergenic 60.0%
CCGATTTCGACTCCGACGAC+TGG - chr4_1:51122898-51122917 Msa0553840:CDS 60.0%
GAAATCGGAGTGGCTGTCGG+CGG + chr4_1:51122943-51122962 None:intergenic 60.0%
GTTGTGGTGAGAGTCGGGTC+GGG + chr4_1:51122989-51123008 None:intergenic 60.0%
GGTTGTGGTGAGAGTCGGGT+CGG + chr4_1:51122990-51123009 None:intergenic 60.0%
GACCCGACTCTCACCACAAC+CGG - chr4_1:51122989-51123008 Msa0553840:CDS 60.0%
GGATTCGCCGGAGAGGTTTC+AGG - chr4_1:51123946-51123965 Msa0553840:intron 60.0%
TTCGCCGGAGAGGTTTCAGG+TGG - chr4_1:51123949-51123968 Msa0553840:intron 60.0%
TGGTGTGAGGGAGAGGATGG+AGG - chr4_1:51124009-51124028 Msa0553840:intron 60.0%
! GGTGTGGGACCAAGAGCACG+AGG - chr4_1:51122190-51122209 Msa0553840:CDS 65.0%
! GTGGTGGAGTGAGGGTCGAG+TGG + chr4_1:51122867-51122886 None:intergenic 65.0%
GCTGTCGGCGGCGGACATTA+AGG + chr4_1:51122931-51122950 None:intergenic 65.0%
! TGGCGTTGGATTCGCCGGAG+AGG - chr4_1:51123939-51123958 Msa0553840:intron 65.0%
ATCGGAGTGGCTGTCGGCGG+CGG + chr4_1:51122940-51122959 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 51121311 51124735 51121311 ID=Msa0553840;Name=Msa0553840
chr4_1 mRNA 51121311 51124735 51121311 ID=Msa0553840-mRNA-1;Parent=Msa0553840;Name=Msa0553840-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|0|0.5|0.5|1|1|2|504|569
chr4_1 exon 51121311 51123494 51121311 ID=Msa0553840-mRNA-1:exon:14578;Parent=Msa0553840-mRNA-1
chr4_1 exon 51124706 51124735 51124706 ID=Msa0553840-mRNA-1:exon:14577;Parent=Msa0553840-mRNA-1
chr4_1 CDS 51124706 51124735 51124706 ID=Msa0553840-mRNA-1:cds;Parent=Msa0553840-mRNA-1
chr4_1 CDS 51121815 51123494 51121815 ID=Msa0553840-mRNA-1:cds;Parent=Msa0553840-mRNA-1
chr4_1 three_prime_UTR 51121311 51121814 51121311 ID=Msa0553840-mRNA-1:three_prime_utr;Parent=Msa0553840-mRNA-1
Gene Sequence

>Msa0553840

ATGCGCAGGCCACATGTTAGAATTTTGAAGGTGGTGGGAATGGCTTACATGTGCGCTGACAGTGGAAACCTAATGGCAATAGCTCAACAAGTCATCAACCAAAAACAACAACAAGAACAACATGAACAACAACAACACCAACAACATCATCATCACCAAAACATCCTAGGTATCACAAACCCTCTCTCTGTCTCACTACATCCATGGCACAACAACAACAACATTCCAGTATCTTCTTCTTCATCACTACCACCTTTGTCTTTTCCACCTACCGATTTCACCGACCCATTTCAAGTCGGGTCGGGTCCTGAAAACACCGATCCCGCTTTCAACTTCCCTTCACCACTCGACCCTCACTCCACCACCTTCCGCTTCTCCGATTTCGACTCCGACGACTGGATGGATACCTTAATGTCCGCCGCCGACAGCCACTCCGATTTCCATCTCAACCCGTTTTCATCATGCCCGACCCGACTCTCACCACAACCGGATCTTCTCTTACCCGCCACCGTACCACTCCAAATCCCAATTCAACCACCCACTCCACAACGCAAAAACGACACTAACACCACCGAAACGACATCGTCGTTTTCATCTTCCACAAACCCACTTCTTAAAACCCTAACAGAAATCGCTTCTTTAATCGAAACCCAAAAACCAAATCAAGCAATTGAAACCCTAACCCATCTCAGTAAATCGATTTCACAAAACGGAAACCCTAATCAAAGAGTTTCCTTTTACTTTTCACAAGCCCTAACCAACAAAATCACCGCACAATCTTCAATAGCTTCTTCAAATTCTTGTTCTACTACATGGGAAGAGTTAACACTCTCTTACAAAGCTCTAAATGACGCATGCCCATATTCAAAGTTCGCACATTTAACAGCAAATCAAGCAATTCTTGAAGCAACAGAAGGATCCAACGATATTCATATAGTTGATTTTGGAATTGTTCAAGGGATTCAATGGGCTGCATTGTTACAAGCATTTGCAACTCGTTCTTCTGGTAAACCTAATAGTGTAAGAATTTCTGGTATACCTGCTATGGCTTTAGGAACTTCGCCAGTTTCATCGATTTCAGCTACCGGTAATCGTTTATCTGAGTTTGCGAAACTTCTAGAGTTAAATTTTGAGTTTACACCGATTCTCACACCTATTGAGCTTCTTGATGAATCAAGCTTTTGTATTCAACCAAATGAGGCTTTAGCTGTTAATTTCATGCTTCAGTTGTATAATCTTTTGGATGAGAATATGAATTCCGTTGAAAAAGCGCTTCGTTTGGCGAAATCATTGAATCCTAAGATTGTTACTTTAGGTGAATATGAAGCTAGTTTGACGACTCGGGTTGGATTTGTTGAAAGATTTGAAACTGCTTTTAATTATTTTGCTGCTTTTTTTGAGAGTTTGGAACCGAACATGGCGTTGGATTCGCCGGAGAGGTTTCAGGTGGAGAGTCTTTTGCTTGGTAGGAGAATTGATGGTGTGATTGGTGTGAGGGAGAGGATGGAGGATAAAGAACAGTGGAAGGTTTTGATGGAAAGCTGTGGTTTTGAATCAGTGGGTTTGAGTCATTATGCAATTAGTCAAGCTAAGATATTGTTGTGGAATTATAGTTATAGTTCTTTGTATTCTCTTGTGGAATCTCAACCTGCTTTTTTGTCTCTGGCTTGGAAAGATGTTCCTCTATTGACTGTTTCTTCTTGGCGTTGA
Protein sequence

>Msa0553840

MRRPHVRILKVVGMAYMCADSGNLMAIAQQVINQKQQQEQHEQQQHQQHHHHQNILGITNPLSVSLHPWHNNNNIPVSSSSSLPPLSFPPTDFTDPFQVGSGPENTDPAFNFPSPLDPHSTTFRFSDFDSDDWMDTLMSAADSHSDFHLNPFSSCPTRLSPQPDLLLPATVPLQIPIQPPTPQRKNDTNTTETTSSFSSSTNPLLKTLTEIASLIETQKPNQAIETLTHLSKSISQNGNPNQRVSFYFSQALTNKITAQSSIASSNSCSTTWEELTLSYKALNDACPYSKFAHLTANQAILEATEGSNDIHIVDFGIVQGIQWAALLQAFATRSSGKPNSVRISGIPAMALGTSPVSSISATGNRLSEFAKLLELNFEFTPILTPIELLDESSFCIQPNEALAVNFMLQLYNLLDENMNSVEKALRLAKSLNPKIVTLGEYEASLTTRVGFVERFETAFNYFAAFFESLEPNMALDSPERFQVESLLLGRRIDGVIGVRERMEDKEQWKVLMESCGFESVGLSHYAISQAKILLWNYSYSSLYSLVESQPAFLSLAWKDVPLLTVSSWR*