AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0687930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0687930 MtrunA17_Chr4g0039661 97.307 557 13 1 14 570 1 555 0.0 1108
Msa0687930 MtrunA17_Chr2g0319091 37.892 351 193 11 234 569 238 578 3.04e-53 190
Msa0687930 MtrunA17_Chr3g0101141 35.593 354 211 10 228 570 195 542 6.49e-53 189
Msa0687930 MtrunA17_Chr5g0445331 34.026 385 233 9 201 570 154 532 1.03e-52 188
Msa0687930 MtrunA17_Chr3g0110971 34.884 387 216 12 205 570 216 587 4.68e-52 187
Msa0687930 MtrunA17_Chr7g0245601 34.060 367 220 10 211 570 441 792 5.51e-52 190
Msa0687930 MtrunA17_Chr7g0224271 31.529 425 235 11 171 570 280 673 2.30e-51 187
Msa0687930 MtrunA17_Chr4g0038761 34.986 363 201 13 222 570 101 442 1.92e-50 180
Msa0687930 MtrunA17_Chr3g0125811 31.383 376 235 8 209 570 204 570 5.47e-50 181
Msa0687930 MtrunA17_Chr2g0306641 32.120 467 263 19 121 570 58 487 1.19e-49 179
Msa0687930 MtrunA17_Chr1g0183641 33.871 372 224 10 205 569 120 476 2.12e-49 178
Msa0687930 MtrunA17_Chr5g0447691 33.684 380 237 9 201 570 170 544 3.78e-49 178
Msa0687930 MtrunA17_Chr1g0159461 31.183 372 241 7 209 570 226 592 5.45e-49 179
Msa0687930 MtrunA17_Chr3g0114841 31.536 371 232 7 218 569 132 499 3.53e-44 164
Msa0687930 MtrunA17_Chr4g0057291 30.287 383 239 12 205 569 289 661 7.31e-44 166
Msa0687930 MtrunA17_Chr4g0057891 27.792 385 249 7 205 570 146 520 2.15e-43 162
Msa0687930 MtrunA17_Chr4g0076091 32.286 350 220 9 231 569 199 542 4.28e-43 162
Msa0687930 MtrunA17_Chr8g0376381 30.952 378 228 14 205 570 166 522 1.29e-42 160
Msa0687930 MtrunA17_Chr1g0200401 31.392 395 232 11 204 570 76 459 2.70e-42 158
Msa0687930 MtrunA17_Chr2g0290241 30.571 350 228 8 231 570 81 425 4.56e-41 154
Msa0687930 MtrunA17_Chr5g0424131 31.232 349 223 6 236 569 152 498 1.41e-38 148
Msa0687930 MtrunA17_Chr3g0085681 28.009 432 239 13 199 570 17 436 4.24e-38 145
Msa0687930 MtrunA17_Chr2g0328341 28.684 380 244 10 206 569 269 637 2.14e-37 147
Msa0687930 MtrunA17_Chr4g0031741 27.937 383 254 9 200 569 276 649 6.71e-37 145
Msa0687930 MtrunA17_Chr2g0328391 26.772 381 254 10 206 569 296 668 1.33e-36 144
Msa0687930 MtrunA17_Chr4g0031721 29.024 379 247 9 205 569 313 683 2.50e-36 144
Msa0687930 MtrunA17_Chr2g0328381 28.684 380 244 10 206 569 272 640 6.57e-36 142
Msa0687930 MtrunA17_Chr4g0031711 27.473 364 238 9 224 569 377 732 7.16e-36 143
Msa0687930 MtrunA17_Chr8g0357601 28.571 385 245 10 205 570 179 552 1.66e-35 140
Msa0687930 MtrunA17_Chr5g0401131 29.771 393 240 13 201 570 111 490 1.81e-35 139
Msa0687930 MtrunA17_Chr5g0396491 25.688 436 244 9 205 570 53 478 2.07e-35 139
Msa0687930 MtrunA17_Chr2g0294461 27.062 388 252 11 205 570 207 585 5.89e-35 139
Msa0687930 MtrunA17_Chr8g0383981 30.730 397 245 15 185 569 117 495 1.63e-34 137
Msa0687930 MtrunA17_Chr4g0038851 26.545 437 238 12 205 570 45 469 2.66e-34 135
Msa0687930 MtrunA17_Chr4g0031701 25.693 397 277 8 177 559 223 615 3.50e-34 137
Msa0687930 MtrunA17_Chr2g0328411 25.729 377 259 7 205 569 286 653 3.90e-34 137
Msa0687930 MtrunA17_Chr2g0294471 26.579 380 261 8 205 570 201 576 4.52e-34 136
Msa0687930 MtrunA17_Chr4g0031731 27.714 350 235 8 224 559 270 615 1.16e-33 135
Msa0687930 MtrunA17_Chr4g0012071 29.639 415 240 16 178 570 167 551 2.63e-33 134
Msa0687930 MtrunA17_Chr2g0328431 25.729 377 259 7 205 569 286 653 1.04e-32 133
Msa0687930 MtrunA17_Chr7g0238891 26.836 354 241 8 231 570 391 740 1.67e-32 132
Msa0687930 MtrunA17_Chr7g0270031 29.076 368 244 10 213 570 194 554 2.31e-32 131
Msa0687930 MtrunA17_Chr2g0328401 27.326 344 229 7 205 535 287 622 2.76e-32 131
Msa0687930 MtrunA17_Chr2g0328361 28.342 374 228 11 206 569 271 614 5.87e-32 130
Msa0687930 MtrunA17_Chr7g0230771 30.667 375 236 12 205 570 372 731 6.18e-31 128
Msa0687930 MtrunA17_Chr7g0230731 29.867 375 239 11 205 570 369 728 3.43e-30 125
Msa0687930 MtrunA17_Chr3g0085361 27.714 350 184 10 278 570 13 350 4.34e-30 121
Msa0687930 MtrunA17_Chr2g0329591 27.735 393 241 11 207 570 73 451 9.18e-29 119
Msa0687930 MtrunA17_Chr2g0328371 29.845 258 158 7 276 520 1 248 3.31e-28 113
Msa0687930 MtrunA17_Chr5g0401421 24.429 438 263 13 195 569 13 445 3.12e-23 102
Msa0687930 MtrunA17_Chr7g0236851 29.017 417 256 16 173 569 260 656 4.73e-23 103
Msa0687930 MtrunA17_Chr4g0053901 23.673 452 276 14 172 569 31 467 7.97e-20 92.8
Msa0687930 MtrunA17_Chr1g0194621 21.271 362 251 10 230 570 170 518 4.77e-19 90.5
Msa0687930 MtrunA17_Chr8g0344101 25.260 384 258 10 209 570 224 600 1.12e-18 89.7
Msa0687930 MtrunA17_Chr8g0373561 25.770 357 217 13 236 570 211 541 7.72e-16 80.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0687930 AT5G66770.1 54.023 609 203 13 14 570 1 584 0.0 537
Msa0687930 AT3G50650.1 50.256 587 217 15 14 570 1 542 8.64e-162 473
Msa0687930 AT5G41920.1 32.843 408 233 12 181 570 16 400 1.04e-54 191
Msa0687930 AT5G48150.1 33.596 381 237 7 201 570 115 490 7.58e-53 188
Msa0687930 AT5G48150.4 33.596 381 237 7 201 570 115 490 7.58e-53 188
Msa0687930 AT5G48150.2 33.596 381 237 7 201 570 115 490 7.58e-53 188
Msa0687930 AT5G48150.3 33.596 381 237 7 201 570 115 490 7.58e-53 188
Msa0687930 AT3G03450.1 34.392 378 224 13 205 570 180 545 1.52e-52 188
Msa0687930 AT1G50600.3 34.211 380 235 8 201 570 152 526 1.82e-52 187
Msa0687930 AT3G54220.1 33.784 370 218 11 211 569 296 649 6.25e-52 189
Msa0687930 AT1G50600.1 34.211 380 235 8 201 570 223 597 8.63e-52 187
Msa0687930 AT1G50600.2 34.211 380 235 8 201 570 227 601 9.85e-52 187
Msa0687930 AT2G04890.1 34.085 355 210 12 227 570 72 413 6.76e-49 175
Msa0687930 AT5G17490.1 32.061 393 244 12 188 570 137 516 1.01e-45 169
Msa0687930 AT1G66350.1 29.149 470 276 16 123 570 72 506 7.48e-45 166
Msa0687930 AT1G21450.1 28.250 400 272 8 181 570 199 593 1.41e-44 167
Msa0687930 AT1G63100.1 31.776 321 184 8 271 569 321 628 6.69e-43 163
Msa0687930 AT1G63100.2 31.776 321 184 8 271 569 346 653 8.42e-43 163
Msa0687930 AT4G17230.1 31.649 376 242 8 205 570 155 525 4.59e-42 159
Msa0687930 AT4G17230.2 31.649 376 242 8 205 570 155 525 4.59e-42 159
Msa0687930 AT2G01570.1 33.141 347 206 14 234 570 251 581 1.51e-41 158
Msa0687930 AT1G55580.1 31.579 380 205 9 238 570 74 445 4.16e-41 154
Msa0687930 AT1G14920.1 31.519 349 209 13 234 570 199 529 2.80e-40 154
Msa0687930 AT3G46600.2 27.763 371 255 7 209 570 5 371 1.20e-39 149
Msa0687930 AT4G08250.1 26.707 498 328 12 87 569 1 476 1.25e-38 148
Msa0687930 AT3G46600.1 27.763 371 255 7 209 570 213 579 1.88e-38 149
Msa0687930 AT3G46600.3 27.763 371 255 7 209 570 181 547 2.12e-38 149
Msa0687930 AT1G07520.2 28.382 377 251 7 209 569 155 528 8.37e-38 147
Msa0687930 AT1G07520.4 28.382 377 251 7 209 569 155 528 8.37e-38 147
Msa0687930 AT1G07520.3 28.382 377 251 7 209 569 320 693 9.30e-38 149
Msa0687930 AT1G07520.1 28.382 377 251 7 209 569 319 692 9.87e-38 148
Msa0687930 AT4G37650.1 29.101 378 238 11 217 570 158 529 1.27e-37 146
Msa0687930 AT5G59450.1 26.551 403 278 8 182 570 200 598 1.82e-37 147
Msa0687930 AT1G07530.1 26.633 398 270 9 186 569 375 764 3.47e-36 144
Msa0687930 AT2G29065.1 27.763 389 239 10 209 569 253 627 1.33e-35 142
Msa0687930 AT2G29060.2 24.524 420 300 9 163 569 239 654 4.87e-35 140
Msa0687930 AT2G29060.1 24.524 420 300 9 163 569 276 691 4.92e-35 140
Msa0687930 AT2G37650.2 25.217 345 236 7 239 570 366 701 6.77e-28 119
Msa0687930 AT2G37650.1 25.217 345 236 7 239 570 378 713 6.96e-28 119
Msa0687930 AT5G52510.1 26.615 387 248 12 207 570 267 640 2.91e-26 114
Msa0687930 AT4G00150.1 28.302 371 219 14 220 570 211 554 6.20e-26 112
Msa0687930 AT1G50420.1 31.278 227 139 5 219 439 71 286 6.88e-24 105
Msa0687930 AT1G50420.1 32.414 145 90 3 431 570 338 479 7.28e-15 77.8
Msa0687930 AT2G45160.1 28.070 399 227 16 205 570 268 639 1.07e-23 105
Msa0687930 AT3G60630.1 29.412 306 190 9 279 570 329 622 2.27e-23 104
Msa0687930 AT3G49950.1 27.171 357 226 12 238 569 60 407 1.24e-22 100
Msa0687930 AT3G13840.1 25.722 381 249 9 207 570 147 510 8.03e-21 96.3
Msa0687930 AT4G36710.1 23.947 380 259 12 205 570 122 485 6.95e-17 84.0

Find 151 sgRNAs with CRISPR-Local

Find 268 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAATAGTGTAAGAATTTC+TGG 0.119456 4_4:+52969623 Msa0687930:CDS
GATATTCATATAGTTGATTT+TGG 0.147557 4_4:+52969536 Msa0687930:CDS
CTCTATTGACTGTTTCTTCT+TGG 0.170351 4_4:+52970293 Msa0687930:CDS
CCAGAAATTCTTACACTATT+AGG 0.205458 4_4:-52969623 None:intergenic
ATATGGGCATGCATCGTTTA+AGG 0.214693 4_4:-52969453 None:intergenic
GCTTCAGTTGTATAATCTTT+TGG 0.233520 4_4:+52969832 Msa0687930:CDS
TAAAAGGAAACTCTTTGATT+AGG 0.234171 4_4:-52969329 None:intergenic
CTCTGTAAATTTGATTTGTC+TGG 0.248126 4_4:+52970756 Msa0687930:three_prime_UTR
GGTATACCTGCTATGGCTTT+AGG 0.252034 4_4:+52969644 Msa0687930:CDS
TCACCTAAAGTAACAATCTT+AGG 0.256338 4_4:-52969908 None:intergenic
AAATGTGCGAACTTTGAATA+TGG 0.257358 4_4:-52969470 None:intergenic
AAAAGGAAACTCTTTGATTA+GGG 0.285344 4_4:-52969328 None:intergenic
TTGATGATATTGGCTCAAAT+TGG 0.286520 4_4:+52970323 Msa0687930:three_prime_UTR
AATCCTAAGATTGTTACTTT+AGG 0.287104 4_4:+52969905 Msa0687930:CDS
GTTGATGTTGCACTGGTATT+AGG 0.301823 4_4:+52970648 Msa0687930:three_prime_UTR
AAGGTTATGGACTTGTAAAA+TGG 0.302281 4_4:+52970684 Msa0687930:three_prime_UTR
AGGGTTTCAATTGCTTGATT+TGG 0.313945 4_4:-52969269 None:intergenic
TAAGGCTTGTGAAAAGTAAA+AGG 0.314801 4_4:-52969345 None:intergenic
TTCGATGAGTGAAGCAATTT+CGG 0.323142 4_4:-52969237 None:intergenic
TATTTGTCTAAATATCAAAA+AGG 0.329898 4_4:+52970786 Msa0687930:three_prime_UTR
TAATTAAATTAATTATCGTT+TGG 0.333699 4_4:+52970532 Msa0687930:three_prime_UTR
TGGCGTTGATTTGATGATAT+TGG 0.335085 4_4:+52970313 Msa0687930:exon
AATTAAATTAATTATCGTTT+GGG 0.335442 4_4:+52970533 Msa0687930:three_prime_UTR
CTTGTTGAGCTATTGCCATT+AGG 0.345192 4_4:-52968677 None:intergenic
TGCGGAGTGGGTGGTTGGAT+TGG 0.345602 4_4:-52969137 None:intergenic
GCGGAGTGGGTGGTTGGATT+GGG 0.350584 4_4:-52969136 None:intergenic
TTGATTTGCTGAGATGGGTT+AGG 0.352847 4_4:-52969289 None:intergenic
TTGTGATACCTAGGATGTTT+TGG 0.353661 4_4:-52968764 None:intergenic
GCATTTGCAACTCGTTCTTC+TGG 0.357528 4_4:+52969596 Msa0687930:CDS
TTAACAGCTAAAGCCTCATT+TGG 0.363944 4_4:-52969803 None:intergenic
TTGAAATGGGTCGGTGAAAT+CGG 0.367983 4_4:-52968883 None:intergenic
GAGTGAGGGTCGAGAGGTGA+AGG 0.387720 4_4:-52968948 None:intergenic
TAGTTCTTTGTATTCTCTTG+TGG 0.398410 4_4:+52970228 Msa0687930:CDS
TGATTTGCTGAGATGGGTTA+GGG 0.400674 4_4:-52969288 None:intergenic
TGAAAACAAAAGAAAGGAAA+AGG 0.401725 4_4:-52970592 None:intergenic
CAAATTCTTCTTCTACTACA+TGG 0.403280 4_4:+52969405 Msa0687930:CDS
CAAAGCAGTGATCAAGGTTA+TGG 0.404632 4_4:+52970671 Msa0687930:three_prime_UTR
TGAAAACGACGATGTCGTTT+CGG 0.406416 4_4:-52969183 None:intergenic
TTGCTTGGTAGGAGAATTGA+TGG 0.408569 4_4:+52970070 Msa0687930:CDS
TGAGTGAAGCAATTTCGGTT+AGG 0.410733 4_4:-52969232 None:intergenic
GAGTGAAGCAATTTCGGTTA+GGG 0.413751 4_4:-52969231 None:intergenic
GAATTGTTCAAGGGATTCAA+TGG 0.414040 4_4:+52969558 Msa0687930:CDS
CTATGCAGATTGGATATGTT+TGG 0.419938 4_4:+52970468 Msa0687930:three_prime_UTR
TATTGGCTCAAATTGGGAAA+AGG 0.422482 4_4:+52970330 Msa0687930:three_prime_UTR
CCGATTTCGACTCCGACGAC+TGG 0.423487 4_4:+52968988 Msa0687930:CDS
GACCCGACTCTCACCACAAC+CGG 0.424081 4_4:+52969079 Msa0687930:CDS
ATTGGCTCAAATTGGGAAAA+GGG 0.425217 4_4:+52970331 Msa0687930:three_prime_UTR
GTACCCGACCCGACTTGAAA+TGG 0.429752 4_4:-52968897 None:intergenic
TGATGATATTGGCTCAAATT+GGG 0.430939 4_4:+52970324 Msa0687930:three_prime_UTR
ATTTGAAGCGGTACGGTGGC+GGG 0.431027 4_4:-52969113 None:intergenic
AGTGATGAAGAAGAAGATAC+TGG 0.431284 4_4:-52968837 None:intergenic
GATTCATCAAGAAGCTCAAT+AGG 0.436690 4_4:-52969764 None:intergenic
GCTGTCGGCGGCGGACATTA+AGG 0.439203 4_4:-52969018 None:intergenic
GTGAAATTGATTTGCTGAGA+TGG 0.442057 4_4:-52969295 None:intergenic
GATTTGAAGCGGTACGGTGG+CGG 0.443082 4_4:-52969114 None:intergenic
GGCGAATCCAACGCCATGTT+CGG 0.446437 4_4:-52970022 None:intergenic
ATCGGAGTGGCTGTCGGCGG+CGG 0.447860 4_4:-52969027 None:intergenic
TTTGGTGATGATGATGTTGT+TGG 0.448206 4_4:-52968746 None:intergenic
CCAGTCGTCGGAGTCGAAAT+CGG 0.452148 4_4:-52968988 None:intergenic
AAACTCAGATAAACGATTAC+CGG 0.454956 4_4:-52969696 None:intergenic
ATCCGGTTGTGGTGAGAGTC+GGG 0.456439 4_4:-52969081 None:intergenic
TCACCGACCCATTTCAAGTC+GGG 0.456993 4_4:+52968889 Msa0687930:CDS
TTCGTTGCGGAGTGGGTGGT+TGG 0.460150 4_4:-52969142 None:intergenic
TCTGTAAATTTGATTTGTCT+GGG 0.465499 4_4:+52970757 Msa0687930:three_prime_UTR
GTGTGATTGGTGTGAGGGAG+AGG 0.470960 4_4:+52970092 Msa0687930:CDS
GTAGCTGAAATCGATGAAAC+TGG 0.471542 4_4:-52969674 None:intergenic
AATTTCTGGTATACCTGCTA+TGG 0.471549 4_4:+52969637 Msa0687930:CDS
GATTGGTGTGAGGGAGAGGA+TGG 0.472918 4_4:+52970096 Msa0687930:CDS
AATGTGCGAACTTTGAATAT+GGG 0.473696 4_4:-52969469 None:intergenic
GAATTTGCAGGTGGTGGGAA+TGG 0.473934 4_4:+52968629 Msa0687930:intron
AGCAAATCAATTTCACAAAA+CGG 0.480108 4_4:+52969302 Msa0687930:CDS
CGCTGACAGTGGAAACCTAA+TGG 0.480605 4_4:+52968662 Msa0687930:CDS
ATCAACTATATGAATATCGT+TGG 0.482770 4_4:-52969531 None:intergenic
CTCTCCACTTGAAACCTCTC+TGG 0.491073 4_4:-52970043 None:intergenic
AGGTTATGGACTTGTAAAAT+GGG 0.491169 4_4:+52970685 Msa0687930:three_prime_UTR
ATCAATTGAAAACAAAAGAA+AGG 0.496213 4_4:-52970598 None:intergenic
AAATTCTTCTTCTACTACAT+GGG 0.496572 4_4:+52969406 Msa0687930:CDS
CGGGTCGGGCATGATGAAAA+CGG 0.497777 4_4:-52969062 None:intergenic
GAAAACAAAAGAAAGGAAAA+GGG 0.498963 4_4:-52970591 None:intergenic
TGAAATTGATTTGCTGAGAT+GGG 0.503445 4_4:-52969294 None:intergenic
AGGAGAATTGATGGTGTGAT+TGG 0.505945 4_4:+52970079 Msa0687930:CDS
ATCGGAGAAACGGAAGGTGG+TGG 0.507100 4_4:-52968970 None:intergenic
AAACGGAAGGTGGTGGAGTG+AGG 0.511325 4_4:-52968963 None:intergenic
AAACGGGTTGAGATGGAAAT+CGG 0.511523 4_4:-52969045 None:intergenic
TACCCGACCCGACTTGAAAT+GGG 0.512685 4_4:-52968896 None:intergenic
GGGTCGGGCATGATGAAAAC+GGG 0.514429 4_4:-52969061 None:intergenic
GACCCATTTCAAGTCGGGTC+GGG 0.515128 4_4:+52968894 Msa0687930:CDS
TTTGGAGCCGAACATGGCGT+TGG 0.516288 4_4:+52970015 Msa0687930:CDS
GCTAGTTTAACGACTCGGGT+TGG 0.521541 4_4:+52969938 Msa0687930:CDS
GGTGAAGAAGCTATGCAGAT+TGG 0.523544 4_4:+52970458 Msa0687930:three_prime_UTR
CATCATCACCAAAACATCCT+AGG 0.524123 4_4:+52968756 Msa0687930:CDS
GGAAAAGGGTTTCTTCCACC+TGG 0.528044 4_4:+52970345 Msa0687930:three_prime_UTR
GATTGGGATTTGAAGCGGTA+CGG 0.528703 4_4:-52969120 None:intergenic
ATTAGGCAAAGCAGTGATCA+AGG 0.531199 4_4:+52970665 Msa0687930:three_prime_UTR
AATTGTTCAAGGGATTCAAT+GGG 0.536168 4_4:+52969559 Msa0687930:CDS
GTTGTGGTGAGAGTCGGGTC+GGG 0.536815 4_4:-52969076 None:intergenic
GTGGCGGGTAAGAGAAGATC+CGG 0.537694 4_4:-52969098 None:intergenic
AGCTCAATAGGCGTAAGAAT+CGG 0.539095 4_4:-52969752 None:intergenic
ATGGACTTGTAAAATGGGAA+TGG 0.542364 4_4:+52970690 Msa0687930:three_prime_UTR
TTTCGACTCCGACGACTGGA+TGG 0.543044 4_4:+52968992 Msa0687930:CDS
CGATGTCGTTTCGGTGGTGT+TGG 0.543827 4_4:-52969174 None:intergenic
GATCCGGTTGTGGTGAGAGT+CGG 0.547170 4_4:-52969082 None:intergenic
CAAGAAGAAACAGTCAATAG+AGG 0.549819 4_4:-52970292 None:intergenic
TGAGAGTTTGGAGCCGAACA+TGG 0.552448 4_4:+52970009 Msa0687930:CDS
TGAAGCTAGTTTAACGACTC+GGG 0.553206 4_4:+52969934 Msa0687930:CDS
ATGAAGCTAGTTTAACGACT+CGG 0.557164 4_4:+52969933 Msa0687930:CDS
ACCTTCAAAATTCTAACATG+TGG 0.560205 4_4:-52967604 None:intergenic
GTGGTGGAGTGAGGGTCGAG+AGG 0.565451 4_4:-52968954 None:intergenic
GTTTCATCGATTTCAGCTAC+CGG 0.566724 4_4:+52969677 Msa0687930:CDS
GAAGTTCCTAAAGCCATAGC+AGG 0.567270 4_4:-52969650 None:intergenic
TCTCTGTCTCACTACATCCA+TGG 0.569966 4_4:+52968793 Msa0687930:CDS
CGGAGTCGAAATCGGAGAAA+CGG 0.570651 4_4:-52968980 None:intergenic
GTAGCATGTTGATGTTGCAC+TGG 0.570751 4_4:+52970641 Msa0687930:three_prime_UTR
AGGGAAGTTGAAAGCGGGAT+CGG 0.573814 4_4:-52968928 None:intergenic
ATGGAAATCGGAGTGGCTGT+CGG 0.577416 4_4:-52969033 None:intergenic
GGATGGAGGATAAAGAACAG+TGG 0.578860 4_4:+52970113 Msa0687930:CDS
AGTGAGGGTCGAGAGGTGAA+GGG 0.581479 4_4:-52968947 None:intergenic
GGTGAAGGGAAGTTGAAAGC+GGG 0.581905 4_4:-52968933 None:intergenic
GGTTGTGGTGAGAGTCGGGT+CGG 0.584555 4_4:-52969077 None:intergenic
AACGGAAGGTGGTGGAGTGA+GGG 0.584958 4_4:-52968962 None:intergenic
TTCGCCAGAGAGGTTTCAAG+TGG 0.588796 4_4:+52970039 Msa0687930:CDS
AGAGAGGGTTTGTGATACCT+AGG 0.592419 4_4:-52968773 None:intergenic
ATGATGAAAACGGGTTGAGA+TGG 0.594967 4_4:-52969052 None:intergenic
CGACCCATTTCAAGTCGGGT+CGG 0.595171 4_4:+52968893 Msa0687930:CDS
GGAGGATAAAGAACAGTGGA+AGG 0.599058 4_4:+52970117 Msa0687930:CDS
GGTTGGATTGGGATTTGAAG+CGG 0.600133 4_4:-52969125 None:intergenic
TGGTGTGAGGGAGAGGATGG+AGG 0.600379 4_4:+52970099 Msa0687930:CDS
GGATGTAGTGAGACAGAGAG+AGG 0.601119 4_4:-52968789 None:intergenic
TTCACCGACCCATTTCAAGT+CGG 0.604924 4_4:+52968888 Msa0687930:CDS
TGATGGTGTGATTGGTGTGA+GGG 0.605734 4_4:+52970087 Msa0687930:CDS
GTCAAGCTAAGATATTGTTG+TGG 0.613839 4_4:+52970194 Msa0687930:CDS
TGGGATTTGAAGCGGTACGG+TGG 0.615546 4_4:-52969117 None:intergenic
TAAGGTATCCATCCAGTCGT+CGG 0.619410 4_4:-52969000 None:intergenic
AAAGAAAGGAAAAGGGTGTG+TGG 0.623975 4_4:-52970584 None:intergenic
AATGGGTCGGTGAAATCGGT+AGG 0.625917 4_4:-52968879 None:intergenic
GCGATTCTTGAAGCAACAGA+AGG 0.626009 4_4:+52969506 Msa0687930:CDS
GGTAAGAGAAGATCCGGTTG+TGG 0.627421 4_4:-52969092 None:intergenic
GAAATCGGAGTGGCTGTCGG+CGG 0.636240 4_4:-52969030 None:intergenic
GATGTAGTGAGACAGAGAGA+GGG 0.639502 4_4:-52968788 None:intergenic
GGGTCGGTGAAATCGGTAGG+TGG 0.649301 4_4:-52968876 None:intergenic
GTCGAAATCGGAGAAACGGA+AGG 0.650056 4_4:-52968976 None:intergenic
GCTTACATGTGCGCTGACAG+TGG 0.651171 4_4:+52968651 Msa0687930:CDS
AGGTGAAGGGAAGTTGAAAG+CGG 0.655548 4_4:-52968934 None:intergenic
GAAATCGGAGAAACGGAAGG+TGG 0.658200 4_4:-52968973 None:intergenic
TTGATGGTGTGATTGGTGTG+AGG 0.659575 4_4:+52970086 Msa0687930:CDS
CGACCCGACTTGAAATGGGT+CGG 0.677848 4_4:-52968892 None:intergenic
TCGGTAGGTGGAAAAGACAA+AGG 0.681946 4_4:-52968864 None:intergenic
TGGCGTTGGATTCGCCAGAG+AGG 0.687403 4_4:+52970029 Msa0687930:CDS
AAACGACGATGTCGTTTCGG+TGG 0.687537 4_4:-52969180 None:intergenic
GGTTGAGATGGAAATCGGAG+TGG 0.716005 4_4:-52969040 None:intergenic
GTAGGTGGAAAAGACAAAGG+TGG 0.720227 4_4:-52968861 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTAAATTAATTATCGTT+TGG + chr4_4:52970532-52970551 Msa0687930:three_prime_UTR 10.0%
!! AATTAAATTAATTATCGTTT+GGG + chr4_4:52970533-52970552 Msa0687930:three_prime_UTR 10.0%
!!! ATTTTCCTTTTTTTTTTTTT+GGG + chr4_4:52970619-52970638 Msa0687930:three_prime_UTR 10.0%
!!! ATGAAAATGTCTTTTAAAAT+GGG - chr4_4:52968314-52968333 None:intergenic 15.0%
!!! AATGAAAATGTCTTTTAAAA+TGG - chr4_4:52968315-52968334 None:intergenic 15.0%
!!! GATTTTCCTTTTTTTTTTTT+TGG + chr4_4:52970618-52970637 Msa0687930:three_prime_UTR 15.0%
!! TATTTGTCTAAATATCAAAA+AGG + chr4_4:52970786-52970805 Msa0687930:three_prime_UTR 15.0%
!! TTTATATGATGTTGTATTGA+GGG + chr4_4:52967882-52967901 Msa0687930:intron 20.0%
!! AAATAAAAAATGTAAGAGAG+TGG + chr4_4:52968261-52968280 Msa0687930:intron 20.0%
!! AATAAAAAATGTAAGAGAGT+GGG + chr4_4:52968262-52968281 Msa0687930:intron 20.0%
!!! GATATTCATATAGTTGATTT+TGG + chr4_4:52969536-52969555 Msa0687930:CDS 20.0%
!! ATCAATTGAAAACAAAAGAA+AGG - chr4_4:52970601-52970620 None:intergenic 20.0%
! ATCTATAAACAACATCTCTT+TGG - chr4_4:52967691-52967710 None:intergenic 25.0%
!! AAAGAGATGTTGTTTATAGA+TGG + chr4_4:52967690-52967709 Msa0687930:intron 25.0%
! GTTTATATGATGTTGTATTG+AGG + chr4_4:52967881-52967900 Msa0687930:intron 25.0%
! CAAAATCCTTATTCTCAAAT+TGG - chr4_4:52967918-52967937 None:intergenic 25.0%
! AACACCAAAGAAATATAAGA+CGG - chr4_4:52968138-52968157 None:intergenic 25.0%
!!! ACTGAATATTTTGAATTTGC+AGG + chr4_4:52968617-52968636 Msa0687930:intron 25.0%
!!! AATTGCTTGATTTGGTTTTT+GGG - chr4_4:52969264-52969283 None:intergenic 25.0%
! AGCAAATCAATTTCACAAAA+CGG + chr4_4:52969302-52969321 Msa0687930:CDS 25.0%
! AAAAGGAAACTCTTTGATTA+GGG - chr4_4:52969331-52969350 None:intergenic 25.0%
! TAAAAGGAAACTCTTTGATT+AGG - chr4_4:52969332-52969351 None:intergenic 25.0%
! AAATTCTTCTTCTACTACAT+GGG + chr4_4:52969406-52969425 Msa0687930:CDS 25.0%
! ATCAACTATATGAATATCGT+TGG - chr4_4:52969534-52969553 None:intergenic 25.0%
!!! TTGATTTTGGAATTGTTCAA+GGG + chr4_4:52969549-52969568 Msa0687930:CDS 25.0%
!!! AATCCTAAGATTGTTACTTT+AGG + chr4_4:52969905-52969924 Msa0687930:CDS 25.0%
!! TCAATTCATAATGTTTTGCT+TGG + chr4_4:52970437-52970456 Msa0687930:three_prime_UTR 25.0%
! GAAAACAAAAGAAAGGAAAA+GGG - chr4_4:52970594-52970613 None:intergenic 25.0%
! TGAAAACAAAAGAAAGGAAA+AGG - chr4_4:52970595-52970614 None:intergenic 25.0%
! TGCTACCCAAAAAAAAAAAA+AGG - chr4_4:52970627-52970646 None:intergenic 25.0%
!!! TCTGTAAATTTGATTTGTCT+GGG + chr4_4:52970757-52970776 Msa0687930:three_prime_UTR 25.0%
ACCTTCAAAATTCTAACATG+TGG - chr4_4:52967607-52967626 None:intergenic 30.0%
AACATCTCTTTGGTTATTGT+TGG - chr4_4:52967681-52967700 None:intergenic 30.0%
CAGGTACAAATGTAATATAG+AGG + chr4_4:52967729-52967748 Msa0687930:intron 30.0%
!! TCTCAATCCCAATTTTGTTT+CGG - chr4_4:52967771-52967790 None:intergenic 30.0%
ATATGTATTCGCAGTAACAA+TGG + chr4_4:52967790-52967809 Msa0687930:intron 30.0%
TGATCTCCAATTTGAGAATA+AGG + chr4_4:52967909-52967928 Msa0687930:intron 30.0%
TTGTCTATACTCAAAGAAAG+CGG + chr4_4:52967935-52967954 Msa0687930:intron 30.0%
! AAACAAAGTGCTTCCAAATT+CGG - chr4_4:52967993-52968012 None:intergenic 30.0%
TTATCATCGTTAAAGTGTGA+GGG - chr4_4:52968078-52968097 None:intergenic 30.0%
ATTATCATCGTTAAAGTGTG+AGG - chr4_4:52968079-52968098 None:intergenic 30.0%
AAATGTGAGTTAGCTAAAGA+TGG - chr4_4:52968102-52968121 None:intergenic 30.0%
TACACCGTCTTATATTTCTT+TGG + chr4_4:52968131-52968150 Msa0687930:intron 30.0%
TTATAGTCGTTCAATGTTCT+AGG + chr4_4:52968335-52968354 Msa0687930:intron 30.0%
!! ATGGTGTAATAGATTGGTTT+GGG - chr4_4:52968517-52968536 None:intergenic 30.0%
!! ATGATGATGGTGTAATAGAT+TGG - chr4_4:52968523-52968542 None:intergenic 30.0%
TAATTAGATGTATGAGAGAG+AGG - chr4_4:52968564-52968583 None:intergenic 30.0%
!! GAATATTTTGAATTTGCAGG+TGG + chr4_4:52968620-52968639 Msa0687930:intron 30.0%
!!! GTTGTTCTTGTTGTTGTTTT+TGG - chr4_4:52968710-52968729 None:intergenic 30.0%
!!! CAATTGCTTGATTTGGTTTT+TGG - chr4_4:52969265-52969284 None:intergenic 30.0%
!! TGAAATTGATTTGCTGAGAT+GGG - chr4_4:52969297-52969316 None:intergenic 30.0%
TAAGGCTTGTGAAAAGTAAA+AGG - chr4_4:52969348-52969367 None:intergenic 30.0%
CAAATTCTTCTTCTACTACA+TGG + chr4_4:52969405-52969424 Msa0687930:CDS 30.0%
AATGTGCGAACTTTGAATAT+GGG - chr4_4:52969472-52969491 None:intergenic 30.0%
AAATGTGCGAACTTTGAATA+TGG - chr4_4:52969473-52969492 None:intergenic 30.0%
!!! GTTGATTTTGGAATTGTTCA+AGG + chr4_4:52969548-52969567 Msa0687930:CDS 30.0%
AATTGTTCAAGGGATTCAAT+GGG + chr4_4:52969559-52969578 Msa0687930:CDS 30.0%
CCAGAAATTCTTACACTATT+AGG - chr4_4:52969626-52969645 None:intergenic 30.0%
CCTAATAGTGTAAGAATTTC+TGG + chr4_4:52969623-52969642 Msa0687930:CDS 30.0%
AAACTCAGATAAACGATTAC+CGG - chr4_4:52969699-52969718 None:intergenic 30.0%
! CTTTTGTATTCAACCAAATG+AGG + chr4_4:52969790-52969809 Msa0687930:CDS 30.0%
! GCTTCAGTTGTATAATCTTT+TGG + chr4_4:52969832-52969851 Msa0687930:CDS 30.0%
TCACCTAAAGTAACAATCTT+AGG - chr4_4:52969911-52969930 None:intergenic 30.0%
TAGTTCTTTGTATTCTCTTG+TGG + chr4_4:52970228-52970247 Msa0687930:CDS 30.0%
!! TTGATGATATTGGCTCAAAT+TGG + chr4_4:52970323-52970342 Msa0687930:three_prime_UTR 30.0%
!! TGATGATATTGGCTCAAATT+GGG + chr4_4:52970324-52970343 Msa0687930:three_prime_UTR 30.0%
! ACTCTGCAATACAAGATTTT+TGG + chr4_4:52970503-52970522 Msa0687930:three_prime_UTR 30.0%
! CTCTGCAATACAAGATTTTT+GGG + chr4_4:52970504-52970523 Msa0687930:three_prime_UTR 30.0%
AAGGTTATGGACTTGTAAAA+TGG + chr4_4:52970684-52970703 Msa0687930:three_prime_UTR 30.0%
AGGTTATGGACTTGTAAAAT+GGG + chr4_4:52970685-52970704 Msa0687930:three_prime_UTR 30.0%
!! CTCTGTAAATTTGATTTGTC+TGG + chr4_4:52970756-52970775 Msa0687930:three_prime_UTR 30.0%
! GCCACATGTTAGAATTTTGA+AGG + chr4_4:52967603-52967622 Msa0687930:CDS 35.0%
! AAGGTTAGCCAATGTAAACA+GGG + chr4_4:52967622-52967641 Msa0687930:intron 35.0%
ATTACATTTGTACCTGCCTT+GGG - chr4_4:52967725-52967744 None:intergenic 35.0%
TATTACATTTGTACCTGCCT+TGG - chr4_4:52967726-52967745 None:intergenic 35.0%
!! TCAATCCCAATTTTGTTTCG+GGG - chr4_4:52967769-52967788 None:intergenic 35.0%
!! CTCAATCCCAATTTTGTTTC+GGG - chr4_4:52967770-52967789 None:intergenic 35.0%
TCAACTTACATCGAACACAA+TGG - chr4_4:52967850-52967869 None:intergenic 35.0%
ATTGTGTTCGATGTAAGTTG+AGG + chr4_4:52967849-52967868 Msa0687930:intron 35.0%
CAACAATGACATACCGAATT+TGG + chr4_4:52967977-52967996 Msa0687930:intron 35.0%
CTAACTTGAGTTCGATTCTT+CGG - chr4_4:52968199-52968218 None:intergenic 35.0%
AATCGAACTCAAGTTAGTTG+CGG + chr4_4:52968202-52968221 Msa0687930:intron 35.0%
AAATCACACACACACTTCAA+CGG + chr4_4:52968409-52968428 Msa0687930:intron 35.0%
GAAGTAGAAAAGAGTGAGTA+AGG - chr4_4:52968463-52968482 None:intergenic 35.0%
! GATGGTGTAATAGATTGGTT+TGG - chr4_4:52968518-52968537 None:intergenic 35.0%
!! GTTATGGTGATTGATGATGA+TGG - chr4_4:52968536-52968555 None:intergenic 35.0%
TCATCATCAATCACCATAAC+AGG + chr4_4:52968536-52968555 Msa0687930:intron 35.0%
!! TATTTTGAATTTGCAGGTGG+TGG + chr4_4:52968623-52968642 Msa0687930:intron 35.0%
! ATTTTGAATTTGCAGGTGGT+GGG + chr4_4:52968624-52968643 Msa0687930:intron 35.0%
!! TTTGGTGATGATGATGTTGT+TGG - chr4_4:52968749-52968768 None:intergenic 35.0%
! TTGTGATACCTAGGATGTTT+TGG - chr4_4:52968767-52968786 None:intergenic 35.0%
AGTGATGAAGAAGAAGATAC+TGG - chr4_4:52968840-52968859 None:intergenic 35.0%
TTCGATGAGTGAAGCAATTT+CGG - chr4_4:52969240-52969259 None:intergenic 35.0%
AGGGTTTCAATTGCTTGATT+TGG - chr4_4:52969272-52969291 None:intergenic 35.0%
!! GTGAAATTGATTTGCTGAGA+TGG - chr4_4:52969298-52969317 None:intergenic 35.0%
!! TGCAGTGATTTTGTTGGTTA+AGG - chr4_4:52969366-52969385 None:intergenic 35.0%
! AGATTGTGCAGTGATTTTGT+TGG - chr4_4:52969372-52969391 None:intergenic 35.0%
GAATTGTTCAAGGGATTCAA+TGG + chr4_4:52969558-52969577 Msa0687930:CDS 35.0%
!! AATTTCTGGTATACCTGCTA+TGG + chr4_4:52969637-52969656 Msa0687930:CDS 35.0%
GATTCATCAAGAAGCTCAAT+AGG - chr4_4:52969767-52969786 None:intergenic 35.0%
TTAACAGCTAAAGCCTCATT+TGG - chr4_4:52969806-52969825 None:intergenic 35.0%
! ATGAAGCTAGTTTAACGACT+CGG + chr4_4:52969933-52969952 Msa0687930:CDS 35.0%
!!! TGCTGCTTTTTTTGAGAGTT+TGG + chr4_4:52969997-52970016 Msa0687930:CDS 35.0%
GTCAAGCTAAGATATTGTTG+TGG + chr4_4:52970194-52970213 Msa0687930:CDS 35.0%
CAAGAAGAAACAGTCAATAG+AGG - chr4_4:52970295-52970314 None:intergenic 35.0%
! CTCTATTGACTGTTTCTTCT+TGG + chr4_4:52970293-52970312 Msa0687930:CDS 35.0%
!! TGGCGTTGATTTGATGATAT+TGG + chr4_4:52970313-52970332 Msa0687930:exon 35.0%
!! TATTGGCTCAAATTGGGAAA+AGG + chr4_4:52970330-52970349 Msa0687930:three_prime_UTR 35.0%
! ATTGGCTCAAATTGGGAAAA+GGG + chr4_4:52970331-52970350 Msa0687930:three_prime_UTR 35.0%
ACACCAGAGAAAAAAAAACC+AGG - chr4_4:52970366-52970385 None:intergenic 35.0%
!!! TGGTTTTTTTTTCTCTGGTG+TGG + chr4_4:52970365-52970384 Msa0687930:three_prime_UTR 35.0%
CTATGCAGATTGGATATGTT+TGG + chr4_4:52970468-52970487 Msa0687930:three_prime_UTR 35.0%
!!! GGGTTGTATTTTTAGCAGTT+TGG + chr4_4:52970553-52970572 Msa0687930:three_prime_UTR 35.0%
ATGGACTTGTAAAATGGGAA+TGG + chr4_4:52970690-52970709 Msa0687930:three_prime_UTR 35.0%
! GAAGGTTAGCCAATGTAAAC+AGG + chr4_4:52967621-52967640 Msa0687930:intron 40.0%
! AGGTTAGCCAATGTAAACAG+GGG + chr4_4:52967623-52967642 Msa0687930:intron 40.0%
TCTTTCCCCCGAAACAAAAT+TGG + chr4_4:52967760-52967779 Msa0687930:intron 40.0%
CTTTCCCCCGAAACAAAATT+GGG + chr4_4:52967761-52967780 Msa0687930:intron 40.0%
!! CAATCCCAATTTTGTTTCGG+GGG - chr4_4:52967768-52967787 None:intergenic 40.0%
TCTATACTCAAAGAAAGCGG+TGG + chr4_4:52967938-52967957 Msa0687930:intron 40.0%
AAAATGTAAGAGAGTGGGTG+TGG + chr4_4:52968267-52968286 Msa0687930:intron 40.0%
AAATGTAAGAGAGTGGGTGT+GGG + chr4_4:52968268-52968287 Msa0687930:intron 40.0%
GTTGAAGTGTGTGTGTGATT+TGG - chr4_4:52968410-52968429 None:intergenic 40.0%
!! GTAATAGATTGGTTTGGGTG+TGG - chr4_4:52968512-52968531 None:intergenic 40.0%
CTTGTTGAGCTATTGCCATT+AGG - chr4_4:52968680-52968699 None:intergenic 40.0%
CATCATCACCAAAACATCCT+AGG + chr4_4:52968756-52968775 Msa0687930:CDS 40.0%
! TTGTTGTTGTTGTTGTGCCA+TGG - chr4_4:52968813-52968832 None:intergenic 40.0%
TTGAAATGGGTCGGTGAAAT+CGG - chr4_4:52968886-52968905 None:intergenic 40.0%
!! AAACGGGTTGAGATGGAAAT+CGG - chr4_4:52969048-52969067 None:intergenic 40.0%
!! ATGATGAAAACGGGTTGAGA+TGG - chr4_4:52969055-52969074 None:intergenic 40.0%
!!! TTGGTGTCGTTTTTTCGTTG+CGG - chr4_4:52969158-52969177 None:intergenic 40.0%
! TGAAAACGACGATGTCGTTT+CGG - chr4_4:52969186-52969205 None:intergenic 40.0%
!!! GGTTTTAAGAAGTGGGTTTG+TGG - chr4_4:52969213-52969232 None:intergenic 40.0%
!!! CGGTTAGGGTTTTAAGAAGT+GGG - chr4_4:52969220-52969239 None:intergenic 40.0%
!!! TCGGTTAGGGTTTTAAGAAG+TGG - chr4_4:52969221-52969240 None:intergenic 40.0%
GAGTGAAGCAATTTCGGTTA+GGG - chr4_4:52969234-52969253 None:intergenic 40.0%
TGAGTGAAGCAATTTCGGTT+AGG - chr4_4:52969235-52969254 None:intergenic 40.0%
!! TGATTTGCTGAGATGGGTTA+GGG - chr4_4:52969291-52969310 None:intergenic 40.0%
!! TTGATTTGCTGAGATGGGTT+AGG - chr4_4:52969292-52969311 None:intergenic 40.0%
ATATGGGCATGCATCGTTTA+AGG - chr4_4:52969456-52969475 None:intergenic 40.0%
GTAGCTGAAATCGATGAAAC+TGG - chr4_4:52969677-52969696 None:intergenic 40.0%
!! GTTTCATCGATTTCAGCTAC+CGG + chr4_4:52969677-52969696 Msa0687930:CDS 40.0%
AGCTCAATAGGCGTAAGAAT+CGG - chr4_4:52969755-52969774 None:intergenic 40.0%
!! TGTTGAAAAAGCGCTTCGTT+TGG + chr4_4:52969871-52969890 Msa0687930:CDS 40.0%
! TGAAGCTAGTTTAACGACTC+GGG + chr4_4:52969934-52969953 Msa0687930:CDS 40.0%
TTGCTTGGTAGGAGAATTGA+TGG + chr4_4:52970070-52970089 Msa0687930:CDS 40.0%
!! AGGAGAATTGATGGTGTGAT+TGG + chr4_4:52970079-52970098 Msa0687930:CDS 40.0%
!! AGAACAGTGGAAGGTTTTGA+TGG + chr4_4:52970126-52970145 Msa0687930:CDS 40.0%
!! AAGGTTTTGATGGAAAGCTG+TGG + chr4_4:52970136-52970155 Msa0687930:CDS 40.0%
!!! AAGCTGTGGTTTTGAATCAG+TGG + chr4_4:52970150-52970169 Msa0687930:CDS 40.0%
!!! AGCTGTGGTTTTGAATCAGT+GGG + chr4_4:52970151-52970170 Msa0687930:CDS 40.0%
!!! TCAACCTGCTTTTTTGTCTC+TGG + chr4_4:52970255-52970274 Msa0687930:CDS 40.0%
CCAGAGAAAAAAAAACCAGG+TGG - chr4_4:52970363-52970382 None:intergenic 40.0%
!!! CCACCTGGTTTTTTTTTCTC+TGG + chr4_4:52970360-52970379 Msa0687930:three_prime_UTR 40.0%
! AAAGAAAGGAAAAGGGTGTG+TGG - chr4_4:52970587-52970606 None:intergenic 40.0%
! GTTGATGTTGCACTGGTATT+AGG + chr4_4:52970648-52970667 Msa0687930:three_prime_UTR 40.0%
ATTAGGCAAAGCAGTGATCA+AGG + chr4_4:52970665-52970684 Msa0687930:three_prime_UTR 40.0%
CAAAGCAGTGATCAAGGTTA+TGG + chr4_4:52970671-52970690 Msa0687930:three_prime_UTR 40.0%
! GGTTAGCCAATGTAAACAGG+GGG + chr4_4:52967624-52967643 Msa0687930:intron 45.0%
GTTTCGGGGGAAAGAAAAAG+AGG - chr4_4:52967755-52967774 None:intergenic 45.0%
TCAAGTTAGTTGCGGACATG+CGG + chr4_4:52968210-52968229 Msa0687930:intron 45.0%
AAGGAAACACTCACTCACTC+GGG - chr4_4:52968369-52968388 None:intergenic 45.0%
CAAGGAAACACTCACTCACT+CGG - chr4_4:52968370-52968389 None:intergenic 45.0%
! ATTTGGAGTGGAGAGAAGAG+AGG - chr4_4:52968393-52968412 None:intergenic 45.0%
! AGTGTGTGTGTGATTTGGAG+TGG - chr4_4:52968405-52968424 None:intergenic 45.0%
AGTGAGTAAGGTGAGAGAGT+GGG - chr4_4:52968451-52968470 None:intergenic 45.0%
TGAGAGAGAGGAACCTGTTA+TGG - chr4_4:52968552-52968571 None:intergenic 45.0%
AGAGAGGGTTTGTGATACCT+AGG - chr4_4:52968776-52968795 None:intergenic 45.0%
GATGTAGTGAGACAGAGAGA+GGG - chr4_4:52968791-52968810 None:intergenic 45.0%
TCTCTGTCTCACTACATCCA+TGG + chr4_4:52968793-52968812 Msa0687930:CDS 45.0%
GTAGGTGGAAAAGACAAAGG+TGG - chr4_4:52968864-52968883 None:intergenic 45.0%
TCGGTAGGTGGAAAAGACAA+AGG - chr4_4:52968867-52968886 None:intergenic 45.0%
! TTCACCGACCCATTTCAAGT+CGG + chr4_4:52968888-52968907 Msa0687930:CDS 45.0%
AGGTGAAGGGAAGTTGAAAG+CGG - chr4_4:52968937-52968956 None:intergenic 45.0%
! TAAGGTATCCATCCAGTCGT+CGG - chr4_4:52969003-52969022 None:intergenic 45.0%
! GATTGGGATTTGAAGCGGTA+CGG - chr4_4:52969123-52969142 None:intergenic 45.0%
! GGTTGGATTGGGATTTGAAG+CGG - chr4_4:52969128-52969147 None:intergenic 45.0%
!!! TCGTTTTTTCGTTGCGGAGT+GGG - chr4_4:52969152-52969171 None:intergenic 45.0%
GCGATTCTTGAAGCAACAGA+AGG + chr4_4:52969506-52969525 Msa0687930:CDS 45.0%
GCATTTGCAACTCGTTCTTC+TGG + chr4_4:52969596-52969615 Msa0687930:CDS 45.0%
GGTATACCTGCTATGGCTTT+AGG + chr4_4:52969644-52969663 Msa0687930:CDS 45.0%
GAAGTTCCTAAAGCCATAGC+AGG - chr4_4:52969653-52969672 None:intergenic 45.0%
! CAAGTGGAGAGTCTTTTGCT+TGG + chr4_4:52970055-52970074 Msa0687930:CDS 45.0%
! TGGAGAGTCTTTTGCTTGGT+AGG + chr4_4:52970059-52970078 Msa0687930:CDS 45.0%
!! TTGATGGTGTGATTGGTGTG+AGG + chr4_4:52970086-52970105 Msa0687930:CDS 45.0%
! TGATGGTGTGATTGGTGTGA+GGG + chr4_4:52970087-52970106 Msa0687930:CDS 45.0%
GGATGGAGGATAAAGAACAG+TGG + chr4_4:52970113-52970132 Msa0687930:CDS 45.0%
GGAGGATAAAGAACAGTGGA+AGG + chr4_4:52970117-52970136 Msa0687930:CDS 45.0%
CAAGCCAGAGACAAAAAAGC+AGG - chr4_4:52970262-52970281 None:intergenic 45.0%
!!! CTGCTTTTTTGTCTCTGGCT+TGG + chr4_4:52970260-52970279 Msa0687930:CDS 45.0%
GGTGAAGAAGCTATGCAGAT+TGG + chr4_4:52970458-52970477 Msa0687930:three_prime_UTR 45.0%
GTAGCATGTTGATGTTGCAC+TGG + chr4_4:52970641-52970660 Msa0687930:three_prime_UTR 45.0%
ACTCGACCCCCTGTTTACAT+TGG - chr4_4:52967633-52967652 None:intergenic 50.0%
TTGTCCCGTCATGGGTAGAA+CGG + chr4_4:52967654-52967673 Msa0687930:intron 50.0%
TTGGACCGTTCTACCCATGA+CGG - chr4_4:52967662-52967681 None:intergenic 50.0%
AAACACTCACTCACTCGGGA+GGG - chr4_4:52968365-52968384 None:intergenic 50.0%
GAGTGAGTAAGGTGAGAGAG+TGG - chr4_4:52968452-52968471 None:intergenic 50.0%
!! GAATTTGCAGGTGGTGGGAA+TGG + chr4_4:52968629-52968648 Msa0687930:intron 50.0%
CGCTGACAGTGGAAACCTAA+TGG + chr4_4:52968662-52968681 Msa0687930:CDS 50.0%
GGATGTAGTGAGACAGAGAG+AGG - chr4_4:52968792-52968811 None:intergenic 50.0%
AATGGGTCGGTGAAATCGGT+AGG - chr4_4:52968882-52968901 None:intergenic 50.0%
! TCACCGACCCATTTCAAGTC+GGG + chr4_4:52968889-52968908 Msa0687930:CDS 50.0%
! TACCCGACCCGACTTGAAAT+GGG - chr4_4:52968899-52968918 None:intergenic 50.0%
AGGGAAGTTGAAAGCGGGAT+CGG - chr4_4:52968931-52968950 None:intergenic 50.0%
GGTGAAGGGAAGTTGAAAGC+GGG - chr4_4:52968936-52968955 None:intergenic 50.0%
GAAATCGGAGAAACGGAAGG+TGG - chr4_4:52968976-52968995 None:intergenic 50.0%
GTCGAAATCGGAGAAACGGA+AGG - chr4_4:52968979-52968998 None:intergenic 50.0%
CGGAGTCGAAATCGGAGAAA+CGG - chr4_4:52968983-52969002 None:intergenic 50.0%
ATGGAAATCGGAGTGGCTGT+CGG - chr4_4:52969036-52969055 None:intergenic 50.0%
GGTTGAGATGGAAATCGGAG+TGG - chr4_4:52969043-52969062 None:intergenic 50.0%
GGTAAGAGAAGATCCGGTTG+TGG - chr4_4:52969095-52969114 None:intergenic 50.0%
!!! TTTTTTCGTTGCGGAGTGGG+TGG - chr4_4:52969149-52969168 None:intergenic 50.0%
!!! GTCGTTTTTTCGTTGCGGAG+TGG - chr4_4:52969153-52969172 None:intergenic 50.0%
! AAACGACGATGTCGTTTCGG+TGG - chr4_4:52969183-52969202 None:intergenic 50.0%
! GCTAGTTTAACGACTCGGGT+TGG + chr4_4:52969938-52969957 Msa0687930:CDS 50.0%
! TGAGAGTTTGGAGCCGAACA+TGG + chr4_4:52970009-52970028 Msa0687930:CDS 50.0%
TTCGCCAGAGAGGTTTCAAG+TGG + chr4_4:52970039-52970058 Msa0687930:CDS 50.0%
CTCTCCACTTGAAACCTCTC+TGG - chr4_4:52970046-52970065 None:intergenic 50.0%
GGAAAAGGGTTTCTTCCACC+TGG + chr4_4:52970345-52970364 Msa0687930:three_prime_UTR 50.0%
! GTCGAGTGTTGTCCCGTCAT+GGG + chr4_4:52967646-52967665 Msa0687930:intron 55.0%
TGGACCGTTCTACCCATGAC+GGG - chr4_4:52967661-52967680 None:intergenic 55.0%
TAGATGGCGAAACGAGCCCA+AGG + chr4_4:52967706-52967725 Msa0687930:intron 55.0%
GAAACACTCACTCACTCGGG+AGG - chr4_4:52968366-52968385 None:intergenic 55.0%
GAGTGGAGAGAAGAGAGGCA+AGG - chr4_4:52968388-52968407 None:intergenic 55.0%
! GCTTACATGTGCGCTGACAG+TGG + chr4_4:52968651-52968670 Msa0687930:CDS 55.0%
! CGACCCGACTTGAAATGGGT+CGG - chr4_4:52968895-52968914 None:intergenic 55.0%
! CGACCCATTTCAAGTCGGGT+CGG + chr4_4:52968893-52968912 Msa0687930:CDS 55.0%
! GACCCATTTCAAGTCGGGTC+GGG + chr4_4:52968894-52968913 Msa0687930:CDS 55.0%
! GTACCCGACCCGACTTGAAA+TGG - chr4_4:52968900-52968919 None:intergenic 55.0%
AGTGAGGGTCGAGAGGTGAA+GGG - chr4_4:52968950-52968969 None:intergenic 55.0%
!! AACGGAAGGTGGTGGAGTGA+GGG - chr4_4:52968965-52968984 None:intergenic 55.0%
!! AAACGGAAGGTGGTGGAGTG+AGG - chr4_4:52968966-52968985 None:intergenic 55.0%
ATCGGAGAAACGGAAGGTGG+TGG - chr4_4:52968973-52968992 None:intergenic 55.0%
CCAGTCGTCGGAGTCGAAAT+CGG - chr4_4:52968991-52969010 None:intergenic 55.0%
TTTCGACTCCGACGACTGGA+TGG + chr4_4:52968992-52969011 Msa0687930:CDS 55.0%
GGGTCGGGCATGATGAAAAC+GGG - chr4_4:52969064-52969083 None:intergenic 55.0%
CGGGTCGGGCATGATGAAAA+CGG - chr4_4:52969065-52969084 None:intergenic 55.0%
ATCCGGTTGTGGTGAGAGTC+GGG - chr4_4:52969084-52969103 None:intergenic 55.0%
GATCCGGTTGTGGTGAGAGT+CGG - chr4_4:52969085-52969104 None:intergenic 55.0%
!! GTGGCGGGTAAGAGAAGATC+CGG - chr4_4:52969101-52969120 None:intergenic 55.0%
! ATTTGAAGCGGTACGGTGGC+GGG - chr4_4:52969116-52969135 None:intergenic 55.0%
! GATTTGAAGCGGTACGGTGG+CGG - chr4_4:52969117-52969136 None:intergenic 55.0%
! TGGGATTTGAAGCGGTACGG+TGG - chr4_4:52969120-52969139 None:intergenic 55.0%
! CGATGTCGTTTCGGTGGTGT+TGG - chr4_4:52969177-52969196 None:intergenic 55.0%
! TTTGGAGCCGAACATGGCGT+TGG + chr4_4:52970015-52970034 Msa0687930:CDS 55.0%
GGCGAATCCAACGCCATGTT+CGG - chr4_4:52970025-52970044 None:intergenic 55.0%
GTGTGATTGGTGTGAGGGAG+AGG + chr4_4:52970092-52970111 Msa0687930:CDS 55.0%
GATTGGTGTGAGGGAGAGGA+TGG + chr4_4:52970096-52970115 Msa0687930:CDS 55.0%
! GGTCGAGTGTTGTCCCGTCA+TGG + chr4_4:52967645-52967664 Msa0687930:intron 60.0%
! TGGGACCAAGAGCACGAGGA+TGG + chr4_4:52968287-52968306 Msa0687930:intron 60.0%
TGGGACCATCCTCGTGCTCT+TGG - chr4_4:52968295-52968314 None:intergenic 60.0%
GGGTCGGTGAAATCGGTAGG+TGG - chr4_4:52968879-52968898 None:intergenic 60.0%
GAGTGAGGGTCGAGAGGTGA+AGG - chr4_4:52968951-52968970 None:intergenic 60.0%
CCGATTTCGACTCCGACGAC+TGG + chr4_4:52968988-52969007 Msa0687930:CDS 60.0%
GAAATCGGAGTGGCTGTCGG+CGG - chr4_4:52969033-52969052 None:intergenic 60.0%
GTTGTGGTGAGAGTCGGGTC+GGG - chr4_4:52969079-52969098 None:intergenic 60.0%
GGTTGTGGTGAGAGTCGGGT+CGG - chr4_4:52969080-52969099 None:intergenic 60.0%
GACCCGACTCTCACCACAAC+CGG + chr4_4:52969079-52969098 Msa0687930:CDS 60.0%
GCGGAGTGGGTGGTTGGATT+GGG - chr4_4:52969139-52969158 None:intergenic 60.0%
TGCGGAGTGGGTGGTTGGAT+TGG - chr4_4:52969140-52969159 None:intergenic 60.0%
TTCGTTGCGGAGTGGGTGGT+TGG - chr4_4:52969145-52969164 None:intergenic 60.0%
! TGGCGTTGGATTCGCCAGAG+AGG + chr4_4:52970029-52970048 Msa0687930:CDS 60.0%
TGGTGTGAGGGAGAGGATGG+AGG + chr4_4:52970099-52970118 Msa0687930:CDS 60.0%
TGGCGAAACGAGCCCAAGGC+AGG + chr4_4:52967710-52967729 Msa0687930:intron 65.0%
! GGTGTGGGACCAAGAGCACG+AGG + chr4_4:52968283-52968302 Msa0687930:intron 65.0%
! GTGGTGGAGTGAGGGTCGAG+AGG - chr4_4:52968957-52968976 None:intergenic 65.0%
GCTGTCGGCGGCGGACATTA+AGG - chr4_4:52969021-52969040 None:intergenic 65.0%
ATCGGAGTGGCTGTCGGCGG+CGG - chr4_4:52969030-52969049 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 52967595 52970826 52967595 ID=Msa0687930;Name=Msa0687930
chr4_4 mRNA 52967595 52970826 52967595 ID=Msa0687930-mRNA-1;Parent=Msa0687930;Name=Msa0687930-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|0|0.5|0.5|1|1|2|505|570
chr4_4 exon 52967595 52967624 52967595 ID=Msa0687930-mRNA-1:exon:13975;Parent=Msa0687930-mRNA-1
chr4_4 exon 52968639 52970826 52968639 ID=Msa0687930-mRNA-1:exon:13976;Parent=Msa0687930-mRNA-1
chr4_4 CDS 52967595 52967624 52967595 ID=Msa0687930-mRNA-1:cds;Parent=Msa0687930-mRNA-1
chr4_4 CDS 52968639 52970321 52968639 ID=Msa0687930-mRNA-1:cds;Parent=Msa0687930-mRNA-1
chr4_4 three_prime_UTR 52970322 52970826 52970322 ID=Msa0687930-mRNA-1:three_prime_utr;Parent=Msa0687930-mRNA-1
Gene Sequence

>Msa0687930

ATGCGCAAGCCACATGTTAGAATTTTGAAGGTGGTGGGAATGGCTTACATGTGCGCTGACAGTGGAAACCTAATGGCAATAGCTCAACAAGTCATCAACCAAAAACAACAACAAGAACAACATGAACAACAACAACACCAACAACATCATCATCACCAAAACATCCTAGGTATCACAAACCCTCTCTCTGTCTCACTACATCCATGGCACAACAACAACAACAACATTCCAGTATCTTCTTCTTCATCACTACCACCTTTGTCTTTTCCACCTACCGATTTCACCGACCCATTTCAAGTCGGGTCGGGTACTGAAAACACCGATCCCGCTTTCAACTTCCCTTCACCTCTCGACCCTCACTCCACCACCTTCCGTTTCTCCGATTTCGACTCCGACGACTGGATGGATACCTTAATGTCCGCCGCCGACAGCCACTCCGATTTCCATCTCAACCCGTTTTCATCATGCCCGACCCGACTCTCACCACAACCGGATCTTCTCTTACCCGCCACCGTACCGCTTCAAATCCCAATCCAACCACCCACTCCGCAACGAAAAAACGACACCAACACCACCGAAACGACATCGTCGTTTTCATCTTCCACAAACCCACTTCTTAAAACCCTAACCGAAATTGCTTCACTCATCGAAACCCAAAAACCAAATCAAGCAATTGAAACCCTAACCCATCTCAGCAAATCAATTTCACAAAACGGAAACCCTAATCAAAGAGTTTCCTTTTACTTTTCACAAGCCTTAACCAACAAAATCACTGCACAATCTTCAATAGCTTCTTCAAATTCTTCTTCTACTACATGGGAAGAGTTAACACTCTCTTACAAAGCCTTAAACGATGCATGCCCATATTCAAAGTTCGCACATTTAACAGCAAATCAAGCGATTCTTGAAGCAACAGAAGGATCCAACGATATTCATATAGTTGATTTTGGAATTGTTCAAGGGATTCAATGGGCTGCATTGTTACAAGCATTTGCAACTCGTTCTTCTGGTAAACCTAATAGTGTAAGAATTTCTGGTATACCTGCTATGGCTTTAGGAACTTCACCAGTTTCATCGATTTCAGCTACCGGTAATCGTTTATCTGAGTTTGCGAAACTTCTAGAGTTAAATTTTGAGTTTACACCGATTCTTACGCCTATTGAGCTTCTTGATGAATCAAGCTTTTGTATTCAACCAAATGAGGCTTTAGCTGTTAATTTCATGCTTCAGTTGTATAATCTTTTGGATGAGAATATGAATTCTGTTGAAAAAGCGCTTCGTTTGGCGAAATCATTGAATCCTAAGATTGTTACTTTAGGTGAATATGAAGCTAGTTTAACGACTCGGGTTGGATTTGTTGAAAGATTTGAAACTGCTTTTAATTATTTTGCTGCTTTTTTTGAGAGTTTGGAGCCGAACATGGCGTTGGATTCGCCAGAGAGGTTTCAAGTGGAGAGTCTTTTGCTTGGTAGGAGAATTGATGGTGTGATTGGTGTGAGGGAGAGGATGGAGGATAAAGAACAGTGGAAGGTTTTGATGGAAAGCTGTGGTTTTGAATCAGTGGGTTTGAGTCATTATGCAATTAGTCAAGCTAAGATATTGTTGTGGAATTATAGTTATAGTTCTTTGTATTCTCTTGTGGAATCTCAACCTGCTTTTTTGTCTCTGGCTTGGAAAGATGTTCCTCTATTGACTGTTTCTTCTTGGCGTTGA
Protein sequence

>Msa0687930

MRKPHVRILKVVGMAYMCADSGNLMAIAQQVINQKQQQEQHEQQQHQQHHHHQNILGITNPLSVSLHPWHNNNNNIPVSSSSSLPPLSFPPTDFTDPFQVGSGTENTDPAFNFPSPLDPHSTTFRFSDFDSDDWMDTLMSAADSHSDFHLNPFSSCPTRLSPQPDLLLPATVPLQIPIQPPTPQRKNDTNTTETTSSFSSSTNPLLKTLTEIASLIETQKPNQAIETLTHLSKSISQNGNPNQRVSFYFSQALTNKITAQSSIASSNSSSTTWEELTLSYKALNDACPYSKFAHLTANQAILEATEGSNDIHIVDFGIVQGIQWAALLQAFATRSSGKPNSVRISGIPAMALGTSPVSSISATGNRLSEFAKLLELNFEFTPILTPIELLDESSFCIQPNEALAVNFMLQLYNLLDENMNSVEKALRLAKSLNPKIVTLGEYEASLTTRVGFVERFETAFNYFAAFFESLEPNMALDSPERFQVESLLLGRRIDGVIGVRERMEDKEQWKVLMESCGFESVGLSHYAISQAKILLWNYSYSSLYSLVESQPAFLSLAWKDVPLLTVSSWR*