AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0554060

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0554060 MtrunA17_Chr4g0039941 93.827 162 9 1 1 161 1 162 1.35e-105 298
Msa0554060 MtrunA17_Chr4g0039921 78.882 161 34 0 1 161 1 161 1.83e-89 258
Msa0554060 MtrunA17_Chr3g0100201 31.746 126 79 2 1 123 1 122 1.11e-15 70.1
Msa0554060 MtrunA17_Chr3g0100191 34.314 102 65 2 27 127 26 126 2.25e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 52 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTAACCCCGTAATATATTT+TGG 0.095607 4_1:-51335818 None:intergenic
GAAGGTGGATACTTAGAATT+TGG 0.200939 4_1:+51337338 Msa0554060:CDS
ATAGGTTATAAAATGATTAT+TGG 0.208282 4_1:+51337699 Msa0554060:three_prime_UTR
TGAGCCATTGGCTAACATTA+TGG 0.279776 4_1:+51337484 Msa0554060:CDS
CTCTTTGGCACCATTGGTAT+TGG 0.293635 4_1:+51335988 Msa0554060:CDS
TAGGTTATAAAATGATTATT+GGG 0.324243 4_1:+51337700 Msa0554060:three_prime_UTR
AGATCCCATGCTTCATTTGC+TGG 0.334877 4_1:-51335967 None:intergenic
TTGCAGCCTTGTTTCTGTTA+AGG 0.338338 4_1:-51337523 None:intergenic
TCCACCTTCTGAAACAATTC+AGG 0.368745 4_1:-51336033 None:intergenic
AATTTCTCTTTGTAACCAGC+TGG 0.370226 4_1:-51337269 None:intergenic
AATAATCCCAAAATATATTA+CGG 0.384290 4_1:+51335811 Msa0554060:five_prime_UTR
ATTTGTTTCCATTGAGCCAT+TGG 0.398190 4_1:+51337472 Msa0554060:CDS
ATAATCCCAAAATATATTAC+GGG 0.418142 4_1:+51335812 Msa0554060:five_prime_UTR
TGCAATCTTGAGAAACATAT+AGG 0.420731 4_1:+51337681 Msa0554060:three_prime_UTR
GTAATTGTAATTTCTAATCA+AGG 0.437838 4_1:-51337740 None:intergenic
TCTTTGTAACCAGCTGGGCC+AGG 0.438011 4_1:-51337263 None:intergenic
TGGGATCTCTTTGGCACCAT+TGG 0.444724 4_1:+51335982 Msa0554060:CDS
AATGAAGCATGGGATCTCTT+TGG 0.446830 4_1:+51335973 Msa0554060:CDS
TTGTGGAAAAGAAAGATGTC+TGG 0.460019 4_1:+51335913 Msa0554060:exon
ATGCTTCATTTGCTGGCACA+TGG 0.468256 4_1:-51335960 None:intergenic
ATTAAATAGATATTTGATGT+AGG 0.470523 4_1:-51337762 None:intergenic
GTTTCTGTTAAGGTAACTCT+TGG 0.474087 4_1:-51337513 None:intergenic
CAGAAGGTGGAGCTAATTGA+AGG 0.500124 4_1:+51336045 Msa0554060:CDS
AATGTTAGCCAATGGCTCAA+TGG 0.500199 4_1:-51337480 None:intergenic
GCCTGAATTGTTTCAGAAGG+TGG 0.512921 4_1:+51336032 Msa0554060:CDS
GTGGAGCTAATTGAAGGAGA+TGG 0.515580 4_1:+51336051 Msa0554060:CDS
ATTGAAGGAGATGGAGGGGT+TGG 0.515980 4_1:+51336060 Msa0554060:CDS
ACAGGTACACCTGGCCCAGC+TGG 0.517233 4_1:+51337254 Msa0554060:intron
AATGGAAACAAATGAGGCAT+TGG 0.521286 4_1:-51337462 None:intergenic
AAAGAAGCGGAGGTAGTTGA+AGG 0.528548 4_1:+51337320 Msa0554060:CDS
ATTGCTAATATATCAATGTA+TGG 0.537251 4_1:-51335851 None:intergenic
GAGCTAATTGAAGGAGATGG+AGG 0.540375 4_1:+51336054 Msa0554060:CDS
ATTTCTCTTTGTAACCAGCT+GGG 0.558097 4_1:-51337268 None:intergenic
TTCAAAATAAGACTAGAGAA+AGG 0.558962 4_1:+51337571 Msa0554060:three_prime_UTR
CTCAAACTCACATTCACTCC+AGG 0.561646 4_1:+51336090 Msa0554060:CDS
GATGCCTGAATTGTTTCAGA+AGG 0.566397 4_1:+51336029 Msa0554060:CDS
GTCTCATTGGACTCAACGTA+AGG 0.570544 4_1:+51335784 None:intergenic
CTACCTCCGCTTCTTTGATG+CGG 0.576020 4_1:-51337313 None:intergenic
ATGTGCCAGCAAATGAAGCA+TGG 0.580036 4_1:+51335962 Msa0554060:CDS
AGCTAATTGAAGGAGATGGA+GGG 0.580245 4_1:+51336055 Msa0554060:CDS
GCTAATTGAAGGAGATGGAG+GGG 0.586950 4_1:+51336056 Msa0554060:CDS
TGTGCCAGCAAATGAAGCAT+GGG 0.591233 4_1:+51335963 Msa0554060:CDS
GGAGTTTGAACAGGTACACC+TGG 0.591936 4_1:+51337245 Msa0554060:intron
TGGCTCAATGGAAACAAATG+AGG 0.595922 4_1:-51337468 None:intergenic
TGAAAACCGCATCAAAGAAG+CGG 0.632918 4_1:+51337307 Msa0554060:CDS
GAAGCGGAGGTAGTTGAAGG+TGG 0.634415 4_1:+51337323 Msa0554060:CDS
ACTTCCATAATGTTAGCCAA+TGG 0.636775 4_1:-51337488 None:intergenic
AAACCGCATCAAAGAAGCGG+AGG 0.668061 4_1:+51337310 Msa0554060:CDS
TGGTATTGGAAAACTCGTTG+AGG 0.676186 4_1:+51336002 Msa0554060:CDS
TCATCTGAGAAATAATACAA+AGG 0.684644 4_1:-51337656 None:intergenic
TAATCCCAAAATATATTACG+GGG 0.696831 4_1:+51335813 Msa0554060:five_prime_UTR
GAGTTACCTTAACAGAAACA+AGG 0.699572 4_1:+51337517 Msa0554060:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTATATGTATGTATTAT+AGG - chr4_1:51336178-51336197 None:intergenic 10.0%
!! GAATTTAACAATAAATTTAA+CGG + chr4_1:51336598-51336617 Msa0554060:intron 10.0%
!!! TTATTAATATACTTTATTTG+GGG - chr4_1:51337104-51337123 None:intergenic 10.0%
!!! TTTTTATATGTATGTATTAT+AGG - chr4_1:51336178-51336197 None:intergenic 10.0%
!! GAATTTAACAATAAATTTAA+CGG + chr4_1:51336598-51336617 Msa0554060:intron 10.0%
!!! TTATTAATATACTTTATTTG+GGG - chr4_1:51337104-51337123 None:intergenic 10.0%
!! AATAATCCCAAAATATATTA+CGG + chr4_1:51335811-51335830 Msa0554060:five_prime_UTR 15.0%
!! TTTATATATCATGAAAAATC+TGG + chr4_1:51336269-51336288 Msa0554060:intron 15.0%
!!! AATGTAAATTGATTTATGTT+TGG + chr4_1:51336557-51336576 Msa0554060:intron 15.0%
!!! CTTAATTGTGTTTTTTTTAT+AGG - chr4_1:51337197-51337216 None:intergenic 15.0%
!! ATAGGTTATAAAATGATTAT+TGG + chr4_1:51337699-51337718 Msa0554060:three_prime_UTR 15.0%
!! TAGGTTATAAAATGATTATT+GGG + chr4_1:51337700-51337719 Msa0554060:three_prime_UTR 15.0%
!! ATTAAATAGATATTTGATGT+AGG - chr4_1:51337765-51337784 None:intergenic 15.0%
!! AATAATCCCAAAATATATTA+CGG + chr4_1:51335811-51335830 Msa0554060:five_prime_UTR 15.0%
!! TTTATATATCATGAAAAATC+TGG + chr4_1:51336269-51336288 Msa0554060:intron 15.0%
!!! AATGTAAATTGATTTATGTT+TGG + chr4_1:51336557-51336576 Msa0554060:intron 15.0%
!!! CTTAATTGTGTTTTTTTTAT+AGG - chr4_1:51337197-51337216 None:intergenic 15.0%
!! ATAGGTTATAAAATGATTAT+TGG + chr4_1:51337699-51337718 Msa0554060:three_prime_UTR 15.0%
!! TAGGTTATAAAATGATTATT+GGG + chr4_1:51337700-51337719 Msa0554060:three_prime_UTR 15.0%
!! ATTAAATAGATATTTGATGT+AGG - chr4_1:51337765-51337784 None:intergenic 15.0%
!! ATAATCCCAAAATATATTAC+GGG + chr4_1:51335812-51335831 Msa0554060:five_prime_UTR 20.0%
!! ATTGCTAATATATCAATGTA+TGG - chr4_1:51335854-51335873 None:intergenic 20.0%
!!! TCAAAAGAGTAAATTTTTTG+TGG + chr4_1:51335896-51335915 Msa0554060:five_prime_UTR 20.0%
!! TTGGAAATTCAATATTTGTT+TGG + chr4_1:51336463-51336482 Msa0554060:intron 20.0%
!! AATACTAAATCCAAATCTAA+AGG + chr4_1:51337148-51337167 Msa0554060:intron 20.0%
!!! ATTGTGTTTTTTTTATAGGA+GGG - chr4_1:51337193-51337212 None:intergenic 20.0%
!!! AATTGTGTTTTTTTTATAGG+AGG - chr4_1:51337194-51337213 None:intergenic 20.0%
!! GTAATTGTAATTTCTAATCA+AGG - chr4_1:51337743-51337762 None:intergenic 20.0%
!! ATAATCCCAAAATATATTAC+GGG + chr4_1:51335812-51335831 Msa0554060:five_prime_UTR 20.0%
!! ATTGCTAATATATCAATGTA+TGG - chr4_1:51335854-51335873 None:intergenic 20.0%
!!! TCAAAAGAGTAAATTTTTTG+TGG + chr4_1:51335896-51335915 Msa0554060:five_prime_UTR 20.0%
!! TTGGAAATTCAATATTTGTT+TGG + chr4_1:51336463-51336482 Msa0554060:intron 20.0%
!! AATACTAAATCCAAATCTAA+AGG + chr4_1:51337148-51337167 Msa0554060:intron 20.0%
!!! ATTGTGTTTTTTTTATAGGA+GGG - chr4_1:51337193-51337212 None:intergenic 20.0%
!!! AATTGTGTTTTTTTTATAGG+AGG - chr4_1:51337194-51337213 None:intergenic 20.0%
!! GTAATTGTAATTTCTAATCA+AGG - chr4_1:51337743-51337762 None:intergenic 20.0%
! TAATCCCAAAATATATTACG+GGG + chr4_1:51335813-51335832 Msa0554060:five_prime_UTR 25.0%
!!! CTCTTTTGAGTTTTAAAATG+TGG - chr4_1:51335885-51335904 None:intergenic 25.0%
!! TTTTCATATCTAGTTGTGTT+AGG - chr4_1:51336155-51336174 None:intergenic 25.0%
! TAAACATGTTTATGTACAAG+TGG + chr4_1:51336354-51336373 Msa0554060:intron 25.0%
! AATCTAGATATTCATGTGTA+TGG + chr4_1:51336402-51336421 Msa0554060:intron 25.0%
!! TGAACAGATAAAGAAACTTT+TGG + chr4_1:51336444-51336463 Msa0554060:intron 25.0%
! TTCTATCGTCAAAATCAATT+CGG + chr4_1:51336675-51336694 Msa0554060:intron 25.0%
!! TGAATTATGTTACTGTGTTT+TGG - chr4_1:51336732-51336751 None:intergenic 25.0%
! ACTCAAAAAACCTTTAGATT+TGG - chr4_1:51337161-51337180 None:intergenic 25.0%
!!! GTAACATTGATTGTTTTGAT+TGG + chr4_1:51337224-51337243 Msa0554060:intron 25.0%
!!! CGTTTTGAAATAATAGAGAA+AGG + chr4_1:51337380-51337399 Msa0554060:CDS 25.0%
! TTCAAAATAAGACTAGAGAA+AGG + chr4_1:51337571-51337590 Msa0554060:three_prime_UTR 25.0%
! TCATCTGAGAAATAATACAA+AGG - chr4_1:51337659-51337678 None:intergenic 25.0%
! TAATCCCAAAATATATTACG+GGG + chr4_1:51335813-51335832 Msa0554060:five_prime_UTR 25.0%
!!! CTCTTTTGAGTTTTAAAATG+TGG - chr4_1:51335885-51335904 None:intergenic 25.0%
!! TTTTCATATCTAGTTGTGTT+AGG - chr4_1:51336155-51336174 None:intergenic 25.0%
! TAAACATGTTTATGTACAAG+TGG + chr4_1:51336354-51336373 Msa0554060:intron 25.0%
! AATCTAGATATTCATGTGTA+TGG + chr4_1:51336402-51336421 Msa0554060:intron 25.0%
!! TGAACAGATAAAGAAACTTT+TGG + chr4_1:51336444-51336463 Msa0554060:intron 25.0%
! TTCTATCGTCAAAATCAATT+CGG + chr4_1:51336675-51336694 Msa0554060:intron 25.0%
!! TGAATTATGTTACTGTGTTT+TGG - chr4_1:51336732-51336751 None:intergenic 25.0%
! ACTCAAAAAACCTTTAGATT+TGG - chr4_1:51337161-51337180 None:intergenic 25.0%
!!! GTAACATTGATTGTTTTGAT+TGG + chr4_1:51337224-51337243 Msa0554060:intron 25.0%
!!! CGTTTTGAAATAATAGAGAA+AGG + chr4_1:51337380-51337399 Msa0554060:CDS 25.0%
! TTCAAAATAAGACTAGAGAA+AGG + chr4_1:51337571-51337590 Msa0554060:three_prime_UTR 25.0%
! TCATCTGAGAAATAATACAA+AGG - chr4_1:51337659-51337678 None:intergenic 25.0%
! CTAACCCCGTAATATATTTT+GGG - chr4_1:51335820-51335839 None:intergenic 30.0%
! ACTAACCCCGTAATATATTT+TGG - chr4_1:51335821-51335840 None:intergenic 30.0%
TAAATAATGTATGACTGACC+TGG - chr4_1:51336111-51336130 None:intergenic 30.0%
CACATGAATATCTAGATTCT+AGG - chr4_1:51336400-51336419 None:intergenic 30.0%
!!! TGTTTTGGTTTCAATTTTGG+AGG - chr4_1:51336717-51336736 None:intergenic 30.0%
!!! CTGTGTTTTGGTTTCAATTT+TGG - chr4_1:51336720-51336739 None:intergenic 30.0%
GAGCTACAAATTACATTAGA+AGG - chr4_1:51336891-51336910 None:intergenic 30.0%
!! ATCATCATTTTCTGTCGTTT+AGG - chr4_1:51336945-51336964 None:intergenic 30.0%
TGCAATCTTGAGAAACATAT+AGG + chr4_1:51337681-51337700 Msa0554060:three_prime_UTR 30.0%
! CTAACCCCGTAATATATTTT+GGG - chr4_1:51335820-51335839 None:intergenic 30.0%
! ACTAACCCCGTAATATATTT+TGG - chr4_1:51335821-51335840 None:intergenic 30.0%
TAAATAATGTATGACTGACC+TGG - chr4_1:51336111-51336130 None:intergenic 30.0%
CACATGAATATCTAGATTCT+AGG - chr4_1:51336400-51336419 None:intergenic 30.0%
!!! TGTTTTGGTTTCAATTTTGG+AGG - chr4_1:51336717-51336736 None:intergenic 30.0%
!!! CTGTGTTTTGGTTTCAATTT+TGG - chr4_1:51336720-51336739 None:intergenic 30.0%
GAGCTACAAATTACATTAGA+AGG - chr4_1:51336891-51336910 None:intergenic 30.0%
!! ATCATCATTTTCTGTCGTTT+AGG - chr4_1:51336945-51336964 None:intergenic 30.0%
TGCAATCTTGAGAAACATAT+AGG + chr4_1:51337681-51337700 Msa0554060:three_prime_UTR 30.0%
TTGTGGAAAAGAAAGATGTC+TGG + chr4_1:51335913-51335932 Msa0554060:exon 35.0%
! AACGAGTTTTCCAATACCAA+TGG - chr4_1:51336001-51336020 None:intergenic 35.0%
CTAAATAGTGAGTCAATCGA+TGG - chr4_1:51336226-51336245 None:intergenic 35.0%
ACATGTTTATGTACAAGTGG+AGG + chr4_1:51336357-51336376 Msa0554060:intron 35.0%
CATGTTTATGTACAAGTGGA+GGG + chr4_1:51336358-51336377 Msa0554060:intron 35.0%
TATAGCGTAGAGCTATCAAA+AGG + chr4_1:51337008-51337027 Msa0554060:intron 35.0%
ATAGCGTAGAGCTATCAAAA+GGG + chr4_1:51337009-51337028 Msa0554060:intron 35.0%
!!! GTTTTGATTGGAGTTTGAAC+AGG + chr4_1:51337236-51337255 Msa0554060:intron 35.0%
ATTTCTCTTTGTAACCAGCT+GGG - chr4_1:51337271-51337290 None:intergenic 35.0%
AATTTCTCTTTGTAACCAGC+TGG - chr4_1:51337272-51337291 None:intergenic 35.0%
! GCGGTTTTCATTATCAATCT+TGG - chr4_1:51337297-51337316 None:intergenic 35.0%
GAAGGTGGATACTTAGAATT+TGG + chr4_1:51337338-51337357 Msa0554060:CDS 35.0%
AATGGAAACAAATGAGGCAT+TGG - chr4_1:51337465-51337484 None:intergenic 35.0%
ATTTGTTTCCATTGAGCCAT+TGG + chr4_1:51337472-51337491 Msa0554060:CDS 35.0%
ACTTCCATAATGTTAGCCAA+TGG - chr4_1:51337491-51337510 None:intergenic 35.0%
GTTTCTGTTAAGGTAACTCT+TGG - chr4_1:51337516-51337535 None:intergenic 35.0%
GAGTTACCTTAACAGAAACA+AGG + chr4_1:51337517-51337536 Msa0554060:CDS 35.0%
TTGTGGAAAAGAAAGATGTC+TGG + chr4_1:51335913-51335932 Msa0554060:exon 35.0%
! AACGAGTTTTCCAATACCAA+TGG - chr4_1:51336001-51336020 None:intergenic 35.0%
CTAAATAGTGAGTCAATCGA+TGG - chr4_1:51336226-51336245 None:intergenic 35.0%
ACATGTTTATGTACAAGTGG+AGG + chr4_1:51336357-51336376 Msa0554060:intron 35.0%
CATGTTTATGTACAAGTGGA+GGG + chr4_1:51336358-51336377 Msa0554060:intron 35.0%
TATAGCGTAGAGCTATCAAA+AGG + chr4_1:51337008-51337027 Msa0554060:intron 35.0%
ATAGCGTAGAGCTATCAAAA+GGG + chr4_1:51337009-51337028 Msa0554060:intron 35.0%
!!! GTTTTGATTGGAGTTTGAAC+AGG + chr4_1:51337236-51337255 Msa0554060:intron 35.0%
ATTTCTCTTTGTAACCAGCT+GGG - chr4_1:51337271-51337290 None:intergenic 35.0%
AATTTCTCTTTGTAACCAGC+TGG - chr4_1:51337272-51337291 None:intergenic 35.0%
! GCGGTTTTCATTATCAATCT+TGG - chr4_1:51337297-51337316 None:intergenic 35.0%
GAAGGTGGATACTTAGAATT+TGG + chr4_1:51337338-51337357 Msa0554060:CDS 35.0%
AATGGAAACAAATGAGGCAT+TGG - chr4_1:51337465-51337484 None:intergenic 35.0%
ATTTGTTTCCATTGAGCCAT+TGG + chr4_1:51337472-51337491 Msa0554060:CDS 35.0%
ACTTCCATAATGTTAGCCAA+TGG - chr4_1:51337491-51337510 None:intergenic 35.0%
GTTTCTGTTAAGGTAACTCT+TGG - chr4_1:51337516-51337535 None:intergenic 35.0%
GAGTTACCTTAACAGAAACA+AGG + chr4_1:51337517-51337536 Msa0554060:CDS 35.0%
AATGAAGCATGGGATCTCTT+TGG + chr4_1:51335973-51335992 Msa0554060:CDS 40.0%
! TGGTATTGGAAAACTCGTTG+AGG + chr4_1:51336002-51336021 Msa0554060:CDS 40.0%
GATGCCTGAATTGTTTCAGA+AGG + chr4_1:51336029-51336048 Msa0554060:CDS 40.0%
!! TCCACCTTCTGAAACAATTC+AGG - chr4_1:51336036-51336055 None:intergenic 40.0%
AGCTAATTGAAGGAGATGGA+GGG + chr4_1:51336055-51336074 Msa0554060:CDS 40.0%
!! ATTTTGGAGGCTCAAAACTG+AGG - chr4_1:51336704-51336723 None:intergenic 40.0%
! CAGCTTCTAGTTACAAGTTG+TGG - chr4_1:51336852-51336871 None:intergenic 40.0%
TGATACCGGTGATTCTAACT+AGG + chr4_1:51337047-51337066 Msa0554060:intron 40.0%
GTCAACCTAGTTAGAATCAC+CGG - chr4_1:51337055-51337074 None:intergenic 40.0%
TGAAAACCGCATCAAAGAAG+CGG + chr4_1:51337307-51337326 Msa0554060:CDS 40.0%
TGGCTCAATGGAAACAAATG+AGG - chr4_1:51337471-51337490 None:intergenic 40.0%
AATGTTAGCCAATGGCTCAA+TGG - chr4_1:51337483-51337502 None:intergenic 40.0%
! TGAGCCATTGGCTAACATTA+TGG + chr4_1:51337484-51337503 Msa0554060:CDS 40.0%
!! TTGCAGCCTTGTTTCTGTTA+AGG - chr4_1:51337526-51337545 None:intergenic 40.0%
AATGAAGCATGGGATCTCTT+TGG + chr4_1:51335973-51335992 Msa0554060:CDS 40.0%
! TGGTATTGGAAAACTCGTTG+AGG + chr4_1:51336002-51336021 Msa0554060:CDS 40.0%
GATGCCTGAATTGTTTCAGA+AGG + chr4_1:51336029-51336048 Msa0554060:CDS 40.0%
!! TCCACCTTCTGAAACAATTC+AGG - chr4_1:51336036-51336055 None:intergenic 40.0%
AGCTAATTGAAGGAGATGGA+GGG + chr4_1:51336055-51336074 Msa0554060:CDS 40.0%
!! ATTTTGGAGGCTCAAAACTG+AGG - chr4_1:51336704-51336723 None:intergenic 40.0%
! CAGCTTCTAGTTACAAGTTG+TGG - chr4_1:51336852-51336871 None:intergenic 40.0%
TGATACCGGTGATTCTAACT+AGG + chr4_1:51337047-51337066 Msa0554060:intron 40.0%
GTCAACCTAGTTAGAATCAC+CGG - chr4_1:51337055-51337074 None:intergenic 40.0%
TGAAAACCGCATCAAAGAAG+CGG + chr4_1:51337307-51337326 Msa0554060:CDS 40.0%
TGGCTCAATGGAAACAAATG+AGG - chr4_1:51337471-51337490 None:intergenic 40.0%
AATGTTAGCCAATGGCTCAA+TGG - chr4_1:51337483-51337502 None:intergenic 40.0%
! TGAGCCATTGGCTAACATTA+TGG + chr4_1:51337484-51337503 Msa0554060:CDS 40.0%
!! TTGCAGCCTTGTTTCTGTTA+AGG - chr4_1:51337526-51337545 None:intergenic 40.0%
! ATGCTTCATTTGCTGGCACA+TGG - chr4_1:51335963-51335982 None:intergenic 45.0%
ATGTGCCAGCAAATGAAGCA+TGG + chr4_1:51335962-51335981 Msa0554060:CDS 45.0%
TGTGCCAGCAAATGAAGCAT+GGG + chr4_1:51335963-51335982 Msa0554060:CDS 45.0%
AGATCCCATGCTTCATTTGC+TGG - chr4_1:51335970-51335989 None:intergenic 45.0%
CTCTTTGGCACCATTGGTAT+TGG + chr4_1:51335988-51336007 Msa0554060:CDS 45.0%
GCCTGAATTGTTTCAGAAGG+TGG + chr4_1:51336032-51336051 Msa0554060:CDS 45.0%
CAGAAGGTGGAGCTAATTGA+AGG + chr4_1:51336045-51336064 Msa0554060:CDS 45.0%
GTGGAGCTAATTGAAGGAGA+TGG + chr4_1:51336051-51336070 Msa0554060:CDS 45.0%
GAGCTAATTGAAGGAGATGG+AGG + chr4_1:51336054-51336073 Msa0554060:CDS 45.0%
GCTAATTGAAGGAGATGGAG+GGG + chr4_1:51336056-51336075 Msa0554060:CDS 45.0%
CTCAAACTCACATTCACTCC+AGG + chr4_1:51336090-51336109 Msa0554060:CDS 45.0%
AAAGAAGCGGAGGTAGTTGA+AGG + chr4_1:51337320-51337339 Msa0554060:CDS 45.0%
! ATGCTTCATTTGCTGGCACA+TGG - chr4_1:51335963-51335982 None:intergenic 45.0%
ATGTGCCAGCAAATGAAGCA+TGG + chr4_1:51335962-51335981 Msa0554060:CDS 45.0%
TGTGCCAGCAAATGAAGCAT+GGG + chr4_1:51335963-51335982 Msa0554060:CDS 45.0%
AGATCCCATGCTTCATTTGC+TGG - chr4_1:51335970-51335989 None:intergenic 45.0%
CTCTTTGGCACCATTGGTAT+TGG + chr4_1:51335988-51336007 Msa0554060:CDS 45.0%
GCCTGAATTGTTTCAGAAGG+TGG + chr4_1:51336032-51336051 Msa0554060:CDS 45.0%
CAGAAGGTGGAGCTAATTGA+AGG + chr4_1:51336045-51336064 Msa0554060:CDS 45.0%
GTGGAGCTAATTGAAGGAGA+TGG + chr4_1:51336051-51336070 Msa0554060:CDS 45.0%
GAGCTAATTGAAGGAGATGG+AGG + chr4_1:51336054-51336073 Msa0554060:CDS 45.0%
GCTAATTGAAGGAGATGGAG+GGG + chr4_1:51336056-51336075 Msa0554060:CDS 45.0%
CTCAAACTCACATTCACTCC+AGG + chr4_1:51336090-51336109 Msa0554060:CDS 45.0%
AAAGAAGCGGAGGTAGTTGA+AGG + chr4_1:51337320-51337339 Msa0554060:CDS 45.0%
!!! TTTATTAATATACTTTATTT+GGG - chr4_1:51337105-51337124 None:intergenic 5.0%
!!! TTTTATTAATATACTTTATT+TGG - chr4_1:51337106-51337125 None:intergenic 5.0%
!! AAATAAAGTATATTAATAAA+AGG + chr4_1:51337105-51337124 Msa0554060:intron 5.0%
!! AATAAAGTATATTAATAAAA+GGG + chr4_1:51337106-51337125 Msa0554060:intron 5.0%
!!! TTTATTAATATACTTTATTT+GGG - chr4_1:51337105-51337124 None:intergenic 5.0%
!!! TTTTATTAATATACTTTATT+TGG - chr4_1:51337106-51337125 None:intergenic 5.0%
!! AAATAAAGTATATTAATAAA+AGG + chr4_1:51337105-51337124 Msa0554060:intron 5.0%
!! AATAAAGTATATTAATAAAA+GGG + chr4_1:51337106-51337125 Msa0554060:intron 5.0%
TGGGATCTCTTTGGCACCAT+TGG + chr4_1:51335982-51336001 Msa0554060:CDS 50.0%
ATTGAAGGAGATGGAGGGGT+TGG + chr4_1:51336060-51336079 Msa0554060:CDS 50.0%
GCGTAGAGCTATCAAAAGGG+TGG + chr4_1:51337012-51337031 Msa0554060:intron 50.0%
TCAAAAGGGTGGCCTCGATT+AGG + chr4_1:51337023-51337042 Msa0554060:intron 50.0%
!! GGCCTCGATTAGGTTGATAC+CGG + chr4_1:51337033-51337052 Msa0554060:intron 50.0%
CACCGGTATCAACCTAATCG+AGG - chr4_1:51337038-51337057 None:intergenic 50.0%
GGAGTTTGAACAGGTACACC+TGG + chr4_1:51337245-51337264 Msa0554060:intron 50.0%
AAACCGCATCAAAGAAGCGG+AGG + chr4_1:51337310-51337329 Msa0554060:CDS 50.0%
CTACCTCCGCTTCTTTGATG+CGG - chr4_1:51337316-51337335 None:intergenic 50.0%
TGGGATCTCTTTGGCACCAT+TGG + chr4_1:51335982-51336001 Msa0554060:CDS 50.0%
ATTGAAGGAGATGGAGGGGT+TGG + chr4_1:51336060-51336079 Msa0554060:CDS 50.0%
GCGTAGAGCTATCAAAAGGG+TGG + chr4_1:51337012-51337031 Msa0554060:intron 50.0%
TCAAAAGGGTGGCCTCGATT+AGG + chr4_1:51337023-51337042 Msa0554060:intron 50.0%
!! GGCCTCGATTAGGTTGATAC+CGG + chr4_1:51337033-51337052 Msa0554060:intron 50.0%
CACCGGTATCAACCTAATCG+AGG - chr4_1:51337038-51337057 None:intergenic 50.0%
GGAGTTTGAACAGGTACACC+TGG + chr4_1:51337245-51337264 Msa0554060:intron 50.0%
AAACCGCATCAAAGAAGCGG+AGG + chr4_1:51337310-51337329 Msa0554060:CDS 50.0%
CTACCTCCGCTTCTTTGATG+CGG - chr4_1:51337316-51337335 None:intergenic 50.0%
TCTTTGTAACCAGCTGGGCC+AGG - chr4_1:51337266-51337285 None:intergenic 55.0%
GAAGCGGAGGTAGTTGAAGG+TGG + chr4_1:51337323-51337342 Msa0554060:CDS 55.0%
TCTTTGTAACCAGCTGGGCC+AGG - chr4_1:51337266-51337285 None:intergenic 55.0%
GAAGCGGAGGTAGTTGAAGG+TGG + chr4_1:51337323-51337342 Msa0554060:CDS 55.0%
ACAGGTACACCTGGCCCAGC+TGG + chr4_1:51337254-51337273 Msa0554060:intron 65.0%
ACAGGTACACCTGGCCCAGC+TGG + chr4_1:51337254-51337273 Msa0554060:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 51335798 51337788 51335798 ID=Msa0554060;Name=Msa0554060
chr4_1 mRNA 51335798 51337788 51335798 ID=Msa0554060-mRNA-1;Parent=Msa0554060;Name=Msa0554060-mRNA-1;_AED=0.26;_eAED=0.26;_QI=130|1|1|1|1|1|2|229|161
chr4_1 exon 51335798 51336111 51335798 ID=Msa0554060-mRNA-1:exon:14660;Parent=Msa0554060-mRNA-1
chr4_1 exon 51337258 51337788 51337258 ID=Msa0554060-mRNA-1:exon:14661;Parent=Msa0554060-mRNA-1
chr4_1 five_prime_UTR 51335798 51335927 51335798 ID=Msa0554060-mRNA-1:five_prime_utr;Parent=Msa0554060-mRNA-1
chr4_1 CDS 51335928 51336111 51335928 ID=Msa0554060-mRNA-1:cds;Parent=Msa0554060-mRNA-1
chr4_1 CDS 51337258 51337559 51337258 ID=Msa0554060-mRNA-1:cds;Parent=Msa0554060-mRNA-1
chr4_1 three_prime_UTR 51337560 51337788 51337560 ID=Msa0554060-mRNA-1:three_prime_utr;Parent=Msa0554060-mRNA-1
Gene Sequence

>Msa0554060

ATGTCTGGTCAACTTGAGCATGAGTTAGAGCTCCATGTGCCAGCAAATGAAGCATGGGATCTCTTTGGCACCATTGGTATTGGAAAACTCGTTGAGGCAGAGATGCCTGAATTGTTTCAGAAGGTGGAGCTAATTGAAGGAGATGGAGGGGTTGGAACTATTCTCAAACTCACATTCACTCCAGGTACACCTGGCCCAGCTGGTTACAAAGAGAAATTCACCAAGATTGATAATGAAAACCGCATCAAAGAAGCGGAGGTAGTTGAAGGTGGATACTTAGAATTTGGATTCACACTATTTAGAGTTCGTTTTGAAATAATAGAGAAAGGTGAAGATTCAAGCATAATCAAAACTACAATAGAGTATGAAATCAAAGAAGAGCATGCAGCCAATGCCTCATTTGTTTCCATTGAGCCATTGGCTAACATTATGGAAGTTGCCAAGAGTTACCTTAACAGAAACAAGGCTGCAAAAGAAGCAAAGTAG
Protein sequence

>Msa0554060

MSGQLEHELELHVPANEAWDLFGTIGIGKLVEAEMPELFQKVELIEGDGGVGTILKLTFTPGTPGPAGYKEKFTKIDNENRIKEAEVVEGGYLEFGFTLFRVRFEIIEKGEDSSIIKTTIEYEIKEEHAANASFVSIEPLANIMEVAKSYLNRNKAAKEAK*