AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0556300

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0556300 MtrunA17_Chr4g0045361 95.476 420 16 1 1 420 1 417 0.0 825
Msa0556300 MtrunA17_Chr4g0062921 39.946 373 163 13 7 367 5 328 2.59e-65 215
Msa0556300 MtrunA17_Chr1g0198601 34.819 359 144 12 44 371 10 309 2.28e-45 161
Msa0556300 MtrunA17_Chr5g0422621 35.942 345 142 12 41 371 16 295 5.89e-45 159
Msa0556300 MtrunA17_Chr5g0402631 58.571 140 51 4 274 410 211 346 7.34e-42 150
Msa0556300 MtrunA17_Chr5g0402631 41.549 142 56 2 8 149 27 141 1.23e-20 92.4
Msa0556300 MtrunA17_Chr1g0175081 47.059 68 36 0 274 341 19 86 2.73e-15 71.2
Msa0556300 MtrunA17_Chr7g0255711 50.746 67 32 1 270 336 84 149 2.86e-15 76.3
Msa0556300 MtrunA17_Chr7g0243081 40.698 86 51 0 245 330 39 124 3.21e-14 73.6
Msa0556300 MtrunA17_Chr2g0288601 50.769 65 32 0 263 327 57 121 4.53e-14 68.9
Msa0556300 MtrunA17_Chr7g0261911 53.846 65 30 0 277 341 262 326 5.60e-14 73.6
Msa0556300 MtrunA17_Chr5g0417521 55.172 58 26 0 274 331 12 69 7.37e-14 72.4
Msa0556300 MtrunA17_Chr1g0190301 61.111 54 21 0 277 330 175 228 1.12e-13 72.0
Msa0556300 MtrunA17_Chr8g0366281 48.980 98 43 4 251 341 142 239 1.23e-13 71.6
Msa0556300 MtrunA17_Chr7g0254981 49.315 73 36 1 269 341 42 113 1.43e-13 70.9
Msa0556300 MtrunA17_Chr2g0304841 48.052 77 35 3 276 351 212 284 1.59e-13 72.4
Msa0556300 MtrunA17_Chr7g0274471 62.963 54 20 0 277 330 218 271 1.89e-13 71.6
Msa0556300 MtrunA17_Chr1g0149841 50.000 68 33 1 272 339 186 252 2.08e-13 72.0
Msa0556300 MtrunA17_Chr5g0400241 47.826 69 35 1 268 336 302 369 2.21e-13 72.0
Msa0556300 MtrunA17_Chr4g0067981 53.731 67 30 1 274 340 212 277 2.42e-13 72.0
Msa0556300 MtrunA17_Chr4g0031961 47.059 68 35 1 273 340 127 193 2.73e-13 70.5
Msa0556300 MtrunA17_Chr4g0054981 32.031 128 75 2 209 336 253 368 3.61e-13 71.2
Msa0556300 MtrunA17_Chr1g0162931 63.265 49 18 0 282 330 23 71 4.04e-13 69.3
Msa0556300 MtrunA17_Chr4g0040481 43.820 89 45 2 273 357 154 241 4.54e-13 70.5
Msa0556300 MtrunA17_Chr1g0210911 61.224 49 19 0 282 330 27 75 7.57e-13 69.3
Msa0556300 MtrunA17_Chr2g0294911 50.746 67 32 1 274 340 208 273 7.71e-13 70.5
Msa0556300 MtrunA17_Chr3g0137101 42.529 87 38 2 276 351 197 282 8.25e-13 70.1
Msa0556300 MtrunA17_Chr2g0291021 60.000 55 22 0 277 331 49 103 8.52e-13 68.9
Msa0556300 MtrunA17_Chr5g0410741 46.465 99 48 2 248 341 187 285 1.31e-12 68.9
Msa0556300 MtrunA17_Chr2g0321461 53.448 58 27 0 274 331 42 99 2.11e-12 68.2
Msa0556300 MtrunA17_Chr6g0461641 51.724 58 28 0 274 331 39 96 2.94e-12 67.8
Msa0556300 MtrunA17_Chr1g0149831 48.438 64 29 1 276 339 127 186 3.40e-12 65.9
Msa0556300 MtrunA17_Chr1g0161431 47.761 67 34 1 274 340 197 262 3.52e-12 68.2
Msa0556300 MtrunA17_Chr6g0479431 41.935 93 47 2 264 349 184 276 4.09e-12 67.4
Msa0556300 MtrunA17_Chr1g0175091 48.529 68 35 0 274 341 255 322 5.44e-12 67.4
Msa0556300 MtrunA17_Chr4g0074411 51.613 62 29 1 274 335 198 258 1.15e-11 66.6
Msa0556300 MtrunA17_Chr8g0356501 55.172 58 26 0 274 331 34 91 1.26e-11 65.5
Msa0556300 MtrunA17_Chr5g0409731 54.098 61 26 1 277 335 25 85 1.28e-11 65.1
Msa0556300 MtrunA17_Chr4g0042451 51.724 58 28 0 274 331 14 71 1.56e-11 65.5
Msa0556300 MtrunA17_Chr4g0009901 49.180 61 30 1 275 335 166 225 2.19e-11 64.3
Msa0556300 MtrunA17_Chr3g0095611 50.000 56 28 0 275 330 17 72 2.36e-11 63.2
Msa0556300 MtrunA17_Chr1g0197171 56.364 55 24 0 277 331 44 98 2.40e-11 64.3
Msa0556300 MtrunA17_Chr2g0324891 49.180 61 30 1 276 336 160 219 2.51e-11 65.1
Msa0556300 MtrunA17_Chr2g0310011 55.172 58 26 0 274 331 32 89 2.51e-11 64.7
Msa0556300 MtrunA17_Chr3g0109981 49.315 73 36 1 269 340 266 338 3.05e-11 65.1
Msa0556300 MtrunA17_Chr2g0320231 45.455 66 36 0 265 330 85 150 4.56e-11 63.9
Msa0556300 MtrunA17_Chr2g0290991 56.364 55 24 0 277 331 39 93 5.20e-11 63.5
Msa0556300 MtrunA17_Chr7g0258551 50.877 57 28 0 274 330 209 265 5.38e-11 63.9
Msa0556300 MtrunA17_Chr1g0154261 41.975 81 45 1 272 352 121 199 6.59e-11 63.2
Msa0556300 MtrunA17_Chr4g0074401 48.387 62 31 1 274 335 200 260 9.64e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0556300 AT2G03500.1 40.533 375 158 10 7 368 5 327 1.41e-64 213
Msa0556300 AT4G37180.1 35.561 374 157 10 9 377 12 306 1.84e-50 174
Msa0556300 AT4G37180.2 35.372 376 162 12 9 377 12 313 2.33e-48 169
Msa0556300 AT4G37180.3 35.737 319 135 8 9 327 12 260 1.12e-43 154
Msa0556300 AT1G49560.1 53.797 158 57 5 261 406 172 325 1.29e-38 142
Msa0556300 AT1G68670.1 69.892 93 20 1 274 358 212 304 7.46e-36 135
Msa0556300 AT1G68670.1 35.000 120 62 4 36 139 8 127 1.18e-11 66.2
Msa0556300 AT1G13300.1 70.787 89 21 2 276 359 180 268 4.35e-34 130
Msa0556300 AT1G25550.1 54.622 119 47 2 274 385 206 324 2.15e-33 128
Msa0556300 AT1G25550.1 33.588 131 67 2 36 146 12 142 9.84e-15 75.5
Msa0556300 AT3G25790.1 57.292 96 36 1 281 371 200 295 1.68e-30 120
Msa0556300 AT1G49560.2 66.667 81 21 2 251 327 164 242 4.97e-25 103
Msa0556300 AT1G25550.2 83.333 54 9 0 274 327 206 259 1.09e-24 103
Msa0556300 AT1G25550.2 33.588 131 67 2 36 146 12 142 1.81e-14 73.9
Msa0556300 AT3G25790.2 78.723 47 10 0 281 327 200 246 1.66e-19 88.2
Msa0556300 AT3G10760.1 50.704 71 34 1 266 336 94 163 2.69e-15 77.0
Msa0556300 AT5G05090.1 50.746 67 32 1 270 336 74 139 2.76e-15 75.9
Msa0556300 AT2G40970.1 50.746 67 32 1 270 336 98 163 4.01e-15 75.1
Msa0556300 AT2G38300.1 46.053 76 41 0 255 330 32 107 1.39e-14 75.1
Msa0556300 AT5G59570.2 50.000 64 31 1 273 336 137 199 1.78e-14 73.9
Msa0556300 AT5G59570.1 50.000 64 31 1 273 336 137 199 1.78e-14 73.9
Msa0556300 AT3G04450.3 50.685 73 35 1 258 330 220 291 1.65e-13 71.2
Msa0556300 AT2G06020.1 35.652 115 72 1 232 346 41 153 1.91e-13 71.2
Msa0556300 AT3G16857.2 43.137 102 44 2 276 377 236 323 3.52e-13 72.0
Msa0556300 AT3G04450.1 43.333 90 50 1 258 347 220 308 3.54e-13 71.2
Msa0556300 AT3G16857.3 43.137 102 44 2 276 377 166 253 3.60e-13 71.6
Msa0556300 AT5G58080.1 50.794 63 30 1 274 336 191 252 3.62e-13 71.6
Msa0556300 AT3G46640.2 48.438 64 32 1 273 336 140 202 3.80e-13 70.5
Msa0556300 AT3G46640.1 48.438 64 32 1 273 336 140 202 3.80e-13 70.5
Msa0556300 AT3G16857.1 43.137 102 44 2 276 377 236 323 4.20e-13 71.6
Msa0556300 AT3G13040.3 58.929 56 23 0 276 331 240 295 4.25e-13 71.2
Msa0556300 AT3G13040.1 58.929 56 23 0 276 331 240 295 4.25e-13 71.2
Msa0556300 AT3G13040.2 58.929 56 23 0 276 331 240 295 4.25e-13 71.2
Msa0556300 AT3G04450.4 43.333 90 50 1 258 347 180 268 4.55e-13 70.9
Msa0556300 AT3G04450.2 43.333 90 50 1 258 347 180 268 4.55e-13 70.9
Msa0556300 AT2G01760.3 54.839 62 27 1 275 336 133 193 5.28e-13 70.1
Msa0556300 AT3G46640.3 48.438 64 32 1 273 336 140 202 5.29e-13 70.1
Msa0556300 AT4G28610.1 52.239 67 31 1 277 342 225 291 5.37e-13 70.5
Msa0556300 AT2G01760.2 54.839 62 27 1 275 336 226 286 6.22e-13 70.5
Msa0556300 AT2G01760.1 54.839 62 27 1 275 336 198 258 6.59e-13 70.1
Msa0556300 AT2G01060.1 60.000 55 22 0 277 331 15 69 7.61e-13 68.9
Msa0556300 AT5G29000.4 56.140 57 25 0 274 330 228 284 1.34e-12 68.6
Msa0556300 AT5G18240.2 55.172 58 26 0 274 331 42 99 1.70e-12 68.9
Msa0556300 AT5G18240.3 55.172 58 26 0 274 331 42 99 1.70e-12 68.9
Msa0556300 AT2G20400.2 50.000 70 34 1 277 345 231 300 1.83e-12 68.9
Msa0556300 AT2G20400.1 53.125 64 29 1 277 340 231 293 1.84e-12 68.9
Msa0556300 AT5G18240.5 55.172 58 26 0 274 331 42 99 1.84e-12 68.9
Msa0556300 AT5G18240.1 55.172 58 26 0 274 331 42 99 1.98e-12 68.9
Msa0556300 AT5G18240.4 55.172 58 26 0 274 331 42 99 1.98e-12 68.9
Msa0556300 AT3G04030.1 53.448 58 27 0 274 331 42 99 2.34e-12 68.6
Msa0556300 AT3G04030.3 53.448 58 27 0 274 331 42 99 2.65e-12 68.6
Msa0556300 AT5G29000.2 59.259 54 22 0 277 330 231 284 3.87e-12 68.2
Msa0556300 AT5G29000.1 59.259 54 22 0 277 330 188 241 3.90e-12 67.8
Msa0556300 AT5G29000.3 59.259 54 22 0 277 330 188 241 3.90e-12 67.8
Msa0556300 AT2G02060.2 50.000 62 31 0 269 330 21 82 5.80e-12 66.2
Msa0556300 AT2G02060.1 50.000 62 31 0 269 330 21 82 5.80e-12 66.2
Msa0556300 AT5G06800.3 44.156 77 40 2 255 331 173 246 6.40e-12 66.2
Msa0556300 AT1G14600.1 51.613 62 30 0 269 330 15 76 6.65e-12 65.9
Msa0556300 AT5G45580.1 48.529 68 35 0 277 344 7 74 7.33e-12 65.9
Msa0556300 AT2G25180.1 46.154 65 34 1 276 340 194 257 7.53e-12 67.8
Msa0556300 AT5G45580.2 47.826 69 36 0 277 345 23 91 8.14e-12 65.9
Msa0556300 AT1G32240.2 45.455 88 47 1 265 351 200 287 8.71e-12 66.6
Msa0556300 AT3G12730.1 55.172 58 26 0 274 331 20 77 9.92e-12 65.1
Msa0556300 AT1G32240.1 45.455 88 47 1 265 351 200 287 1.13e-11 66.6
Msa0556300 AT5G16560.1 50.000 78 37 2 265 340 206 283 2.39e-11 65.5
Msa0556300 AT1G67710.3 51.724 58 28 0 279 336 121 178 2.70e-11 65.5
Msa0556300 AT1G67710.2 51.724 58 28 0 279 336 130 187 2.73e-11 65.5
Msa0556300 AT1G67710.1 51.724 58 28 0 279 336 195 252 3.70e-11 65.5
Msa0556300 AT5G06800.2 44.156 77 40 2 255 331 173 246 4.14e-11 64.7
Msa0556300 AT1G79430.2 55.172 58 26 0 274 331 31 88 4.18e-11 64.7
Msa0556300 AT3G24120.1 55.172 58 26 0 274 331 38 95 4.41e-11 63.9
Msa0556300 AT5G06800.1 44.156 77 40 2 255 331 173 246 4.60e-11 64.7
Msa0556300 AT4G13640.2 56.364 55 24 0 277 331 37 91 5.10e-11 63.9
Msa0556300 AT4G13640.1 56.364 55 24 0 277 331 37 91 5.21e-11 63.5
Msa0556300 AT3G24120.2 55.172 58 26 0 274 331 38 95 5.28e-11 63.5
Msa0556300 AT2G42660.1 55.102 49 22 0 282 330 55 103 8.33e-11 62.8
Msa0556300 AT2G40260.1 46.154 65 35 0 266 330 71 135 8.49e-11 63.9

Find 137 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTAGTTCTTGGAGGTTTA+TGG 0.186325 4_1:+54436953 Msa0556300:CDS
GTGATGAGATTTATATAAAA+TGG 0.205392 4_1:-54434609 None:intergenic
CTCTTTCCTTTCTGCATTGT+AGG 0.213979 4_1:+54437703 Msa0556300:three_prime_UTR
ATTCTTGTTGAGGAGTTAAA+AGG 0.219449 4_1:-54434652 None:intergenic
TGACAGAATTTGCCCTTAAA+AGG 0.238192 4_1:-54437680 None:intergenic
GGTTCTTCCTTCTCTTCTTT+AGG 0.247640 4_1:-54435664 None:intergenic
TGGACTAATAATATCTCTTC+TGG 0.271849 4_1:-54437437 None:intergenic
TTTCTGCATTGTAGGGTTGT+AGG 0.291103 4_1:+54437711 Msa0556300:three_prime_UTR
GAGGAAATTAGCGATGGTTT+TGG 0.303370 4_1:-54434798 None:intergenic
TTCAGCCGCAGCAGACTTCT+AGG 0.305420 4_1:+54435833 Msa0556300:CDS
GATCAGTCTGTTGTAGTTCT+TGG 0.310407 4_1:+54436943 Msa0556300:CDS
ACAGAAGAATACGGTGAAAA+AGG 0.316862 4_1:-54435613 None:intergenic
TGAATTTAGGCCGTTAGATT+TGG 0.321583 4_1:-54434703 None:intergenic
ACAAATCTCCGGTGCAATTC+CGG 0.337882 4_1:-54435877 None:intergenic
AAAGAGAGCCTATTCCTTGC+AGG 0.346901 4_1:-54435685 None:intergenic
ATTAGTCCAATGTTATGTTT+TGG 0.349670 4_1:+54437451 Msa0556300:three_prime_UTR
GGAATAAACTCTTCCAAAAC+TGG 0.355348 4_1:-54435182 None:intergenic
TAGATGTGAATGTGAAATTA+AGG 0.363270 4_1:-54434493 None:intergenic
ATGGAGTTTGGAGAGTTTAT+CGG 0.366175 4_1:-54434849 None:intergenic
ATGAGTTCTCTAATTTGCTT+TGG 0.366259 4_1:-54436520 None:intergenic
AGAGTTTATCGGAGGTTGTT+TGG 0.367071 4_1:-54434838 None:intergenic
TTCTGCATTGTAGGGTTGTA+GGG 0.369021 4_1:+54437712 Msa0556300:three_prime_UTR
AGAGAGGAAATCATGGAGTT+TGG 0.369125 4_1:-54434861 None:intergenic
TACTACTACAAGATTGAAAA+AGG 0.370440 4_1:-54435564 None:intergenic
GATCGAGAAACAGTTCTAAC+AGG 0.377734 4_1:+54435755 Msa0556300:CDS
GCAGCAAAGATAAGAAGAAT+TGG 0.382485 4_1:+54435276 Msa0556300:CDS
AGAGAACTCATGCAAGTTGA+TGG 0.388920 4_1:+54436532 Msa0556300:CDS
ATGAGAGCCAGACCACAAAA+TGG 0.396148 4_1:-54437634 None:intergenic
GGATGAGTTCTGTTCAGTTA+TGG 0.403657 4_1:+54435297 Msa0556300:CDS
CGCATACTCGGCTGCACGGT+TGG 0.404437 4_1:-54435799 None:intergenic
AAGAGTCTGAGGGAAGGATT+TGG 0.407195 4_1:+54435724 Msa0556300:CDS
TGATGAGATTTATATAAAAT+GGG 0.411321 4_1:-54434608 None:intergenic
AGGAAGAACCTGCAAGGAAT+AGG 0.421189 4_1:+54435677 Msa0556300:CDS
AATACGAACCGTCGTTTAAG+AGG 0.421808 4_1:-54434946 None:intergenic
TTCTTGTTGAGGAGTTAAAA+GGG 0.425040 4_1:-54434651 None:intergenic
GGACTAATAATATCTCTTCT+GGG 0.425648 4_1:-54437436 None:intergenic
CAGTCTGTTGTAGTTCTTGG+AGG 0.429416 4_1:+54436946 Msa0556300:CDS
AGACGCCGTACTGCTACCTT+TGG 0.431495 4_1:-54437004 None:intergenic
AATAATGTAATAAGAAGAAG+TGG 0.434366 4_1:-54434518 None:intergenic
TTCATTTAGCTACAGGATCC+CGG 0.434371 4_1:+54437046 Msa0556300:CDS
TCTTTCCTTTCTGCATTGTA+GGG 0.437001 4_1:+54437704 Msa0556300:three_prime_UTR
AGAGTATCGGTTCTCATAGT+AGG 0.438676 4_1:+54437751 Msa0556300:three_prime_UTR
TACATTCCACGATAAGAATC+AGG 0.439928 4_1:+54437358 Msa0556300:three_prime_UTR
ATCGGAGGTTGTTTGGATTG+TGG 0.443195 4_1:-54434831 None:intergenic
TCTTCACTTCATCATTAGTC+AGG 0.447623 4_1:-54436555 None:intergenic
GAAGAAGAAGATTCTTGTTG+AGG 0.451264 4_1:-54434662 None:intergenic
CATCTCTGAAATTTGTGAAG+TGG 0.455715 4_1:-54437513 None:intergenic
ACATGGAAAAGTTGGTGTAT+AGG 0.458002 4_1:+54437158 Msa0556300:CDS
GAATACATAGAAGAGGCTAT+TGG 0.458107 4_1:+54437260 Msa0556300:three_prime_UTR
GTTAATGCGTTGCAGAAGCT+TGG 0.458197 4_1:+54435898 Msa0556300:CDS
GTAGGACTAGGAGAAGATAA+TGG 0.459925 4_1:-54437800 None:intergenic
TCCCTCAGACTCTTCACCTC+AGG 0.461715 4_1:-54435715 None:intergenic
CAGTTGGCGATGTCCCTCCC+CGG 0.465539 4_1:-54437065 None:intergenic
AGTCCACTTCATTTAGCTAC+AGG 0.467143 4_1:+54437039 Msa0556300:CDS
TCTTGTAGTAGTAATAGAAA+TGG 0.468798 4_1:+54435574 Msa0556300:CDS
AAAGGTAGCAGTACGGCGTC+TGG 0.473278 4_1:+54437006 Msa0556300:CDS
TGATCAATCTCTTTCTTCAA+AGG 0.473313 4_1:-54435203 None:intergenic
AAATCTAAACTGAGTTCTGC+AGG 0.491660 4_1:-54434758 None:intergenic
TGAGGGAAGGATTTGGTTCG+AGG 0.493121 4_1:+54435731 Msa0556300:CDS
ATTCACAGACATGGAAAAGT+TGG 0.497674 4_1:+54437150 Msa0556300:CDS
AGCGGCGCAGCACGCATACT+CGG 0.508461 4_1:-54435811 None:intergenic
TCATTTAGCTACAGGATCCC+GGG 0.515125 4_1:+54437047 Msa0556300:CDS
ATCAGCACCTAAAGAAGAGA+AGG 0.517481 4_1:+54435657 Msa0556300:CDS
TGATTCTTATCGTGGAATGT+AGG 0.518021 4_1:-54437356 None:intergenic
TGTAGTAGTAATAGAAATGG+AGG 0.522008 4_1:+54435577 Msa0556300:CDS
CAGAAGCTTGGAGGTTCTCA+AGG 0.522479 4_1:+54435910 Msa0556300:CDS
TTTGCAGATAAAAGTATACA+TGG 0.523808 4_1:+54437203 Msa0556300:three_prime_UTR
CTAGGAAACAGAGGAGATGC+TGG 0.524226 4_1:+54435851 Msa0556300:CDS
CGAGTCGAGAGAGGAAATCA+TGG 0.525149 4_1:-54434868 None:intergenic
CTCCTGAGGTGAAGAGTCTG+AGG 0.527871 4_1:+54435713 Msa0556300:CDS
GTGATAGATAGAGTAGGACT+AGG 0.528949 4_1:-54437812 None:intergenic
GAGTCGTTGTATTGTTCTTG+AGG 0.536314 4_1:-54436979 None:intergenic
GAAGTCTGCTGCGGCTGAAG+CGG 0.537379 4_1:-54435829 None:intergenic
TGATCAGTACCACTTGCAGC+TGG 0.538762 4_1:-54436925 None:intergenic
TAATGGATCCTCTACGGTTG+CGG 0.540015 4_1:-54437783 None:intergenic
ATACTCGGCTGCACGGTTGG+AGG 0.545035 4_1:-54435796 None:intergenic
AGTTGGCGATGTCCCTCCCC+GGG 0.547122 4_1:-54437064 None:intergenic
GCTAAATGAAGTGGACTTTG+TGG 0.551266 4_1:-54437033 None:intergenic
AATGCGTTGCAGAAGCTTGG+AGG 0.554542 4_1:+54435901 Msa0556300:CDS
AGAGAAAGCAACGACGAATG+AGG 0.554931 4_1:-54434406 None:intergenic
CATCCACCAAAACATAACAT+TGG 0.559517 4_1:-54437457 None:intergenic
ACAGAGGAGATGCTGGTCAC+CGG 0.560535 4_1:+54435858 Msa0556300:CDS
GAACTCTCCGCGTATGAAGT+CGG 0.562568 4_1:-54436903 None:intergenic
AAATGACCTGATTCTTATCG+TGG 0.563659 4_1:-54437364 None:intergenic
ATTTATATAAAATGGGAATG+AGG 0.564588 4_1:-54434601 None:intergenic
TCTTGTTGAGGAGTTAAAAG+GGG 0.565631 4_1:-54434650 None:intergenic
GCTGGTCACCGGAATTGCAC+CGG 0.565693 4_1:+54435869 Msa0556300:CDS
TAGCTACAGGATCCCGGGGA+GGG 0.570717 4_1:+54437052 Msa0556300:CDS
GCAACGCATTAACAAATCTC+CGG 0.573053 4_1:-54435888 None:intergenic
AGAAGATAATGGATCCTCTA+CGG 0.579715 4_1:-54437789 None:intergenic
GGAGGGACATCGCCAACTGA+AGG 0.584248 4_1:+54437069 Msa0556300:CDS
GTCACAGGAACAGAAGAATA+CGG 0.587549 4_1:-54435622 None:intergenic
GTTTGATTTAGAAAGAGTAT+CGG 0.588174 4_1:+54437738 Msa0556300:three_prime_UTR
TCCATGCTCCTCTTAAACGA+CGG 0.593486 4_1:+54434938 Msa0556300:CDS
AATACATGAATACATAGAAG+AGG 0.594019 4_1:+54437253 Msa0556300:three_prime_UTR
ACAATTTGTGACTGCAGCTG+AGG 0.594577 4_1:+54435540 Msa0556300:CDS
GCAGGAACAACACAACCCAT+TGG 0.594618 4_1:-54434740 None:intergenic
GCCAACTGAAGGTGATAGCA+TGG 0.596672 4_1:+54437080 Msa0556300:CDS
ATGATAGAGGAAATTAGCGA+TGG 0.597212 4_1:-54434804 None:intergenic
TGACAGAAAAGGGAGGAAGA+AGG 0.597320 4_1:+54435517 Msa0556300:intron
TCTTAATAAATTAGAAACAA+AGG 0.606924 4_1:+54435385 Msa0556300:CDS
GATCCTGTAGCTAAATGAAG+TGG 0.608872 4_1:-54437042 None:intergenic
CATCAACGATCCAAATCTAA+CGG 0.610204 4_1:+54434693 Msa0556300:five_prime_UTR
TCCATGCTATCACCTTCAGT+TGG 0.614177 4_1:-54437081 None:intergenic
GACTAATAATATCTCTTCTG+GGG 0.614864 4_1:-54437435 None:intergenic
TGAATGTGAAATTAAGGATG+CGG 0.615466 4_1:-54434487 None:intergenic
TACAACCCTACAATGCAGAA+AGG 0.616109 4_1:-54437709 None:intergenic
AAAAGTCATATTCACAGACA+TGG 0.616407 4_1:+54437141 Msa0556300:CDS
GATAATGTCACTGTTGTCAC+AGG 0.616977 4_1:-54435637 None:intergenic
AGCACGCATACTCGGCTGCA+CGG 0.618042 4_1:-54435803 None:intergenic
GTTCATCTTCGAGTCGAGAG+AGG 0.619371 4_1:-54434877 None:intergenic
GGATTGTGGAAAGATGATAG+AGG 0.619765 4_1:-54434817 None:intergenic
AAGAGAAGGAAGAACCTGCA+AGG 0.620171 4_1:+54435671 Msa0556300:CDS
CAATACAACGACTCTTCCAA+AGG 0.620750 4_1:+54436988 Msa0556300:CDS
TCCTGAGGTGAAGAGTCTGA+GGG 0.627907 4_1:+54435714 Msa0556300:CDS
GAGGGAAGGATTTGGTTCGA+GGG 0.630808 4_1:+54435732 Msa0556300:CDS
TTTATCTGCAAAATTCATGG+TGG 0.631088 4_1:-54437192 None:intergenic
GAGGTGAAGAGTCTGAGGGA+AGG 0.631423 4_1:+54435718 Msa0556300:CDS
TTGCAGATAAAAGTATACAT+GGG 0.633381 4_1:+54437204 Msa0556300:three_prime_UTR
GAGTTTGGAGAGTTTATCGG+AGG 0.638169 4_1:-54434846 None:intergenic
CGGAGAGTTCCAGCTGCAAG+TGG 0.640628 4_1:+54436916 Msa0556300:CDS
TTTAAGAGGAGCATGGAGAG+AGG 0.641505 4_1:-54434932 None:intergenic
CTCTTCCAAAGGTAGCAGTA+CGG 0.648767 4_1:+54436999 Msa0556300:CDS
TCACAAGTGATAGATAGAGT+AGG 0.649562 4_1:-54437818 None:intergenic
ATAATGTAATAAGAAGAAGT+GGG 0.650074 4_1:-54434517 None:intergenic
AGGGAAGGATTTGGTTCGAG+GGG 0.651342 4_1:+54435733 Msa0556300:CDS
CAGATAAAAGTATACATGGG+AGG 0.654402 4_1:+54437207 Msa0556300:three_prime_UTR
ACTTCATACCGCAACCGTAG+AGG 0.656491 4_1:+54437775 Msa0556300:three_prime_UTR
AGCAGACTTCTAGGAAACAG+AGG 0.657415 4_1:+54435842 Msa0556300:CDS
CATTTAGCTACAGGATCCCG+GGG 0.660202 4_1:+54437048 Msa0556300:CDS
ACCGTCGTTTAAGAGGAGCA+TGG 0.663203 4_1:-54434939 None:intergenic
AGAAATACCGACTTCATACG+CGG 0.668200 4_1:+54436896 Msa0556300:intron
ACCACGACAGGAAAACACAA+AGG 0.668802 4_1:-54437570 None:intergenic
ATCGAGAAACAGTTCTAACA+GGG 0.670598 4_1:+54435756 Msa0556300:CDS
TGTTTCCTAGAAGTCTGCTG+CGG 0.688682 4_1:-54435838 None:intergenic
TTAGCTACAGGATCCCGGGG+AGG 0.690072 4_1:+54437051 Msa0556300:CDS
TTGGTGATGACAGAAAAGGG+AGG 0.712344 4_1:+54435510 Msa0556300:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATGAGATTTATATAAAAT+GGG - chr4_1:54434611-54434630 None:intergenic 15.0%
!! TCTTAATAAATTAGAAACAA+AGG + chr4_1:54435385-54435404 Msa0556300:CDS 15.0%
!!! TTTTTTCTTGTCTAATTTTT+AGG - chr4_1:54435454-54435473 None:intergenic 15.0%
!! AATCTATAATATTTCCAAAT+TGG - chr4_1:54436055-54436074 None:intergenic 15.0%
!! ATAATGTAATAAGAAGAAGT+GGG - chr4_1:54434520-54434539 None:intergenic 20.0%
!! AATAATGTAATAAGAAGAAG+TGG - chr4_1:54434521-54434540 None:intergenic 20.0%
!! ATTTATATAAAATGGGAATG+AGG - chr4_1:54434604-54434623 None:intergenic 20.0%
!! GTGATGAGATTTATATAAAA+TGG - chr4_1:54434612-54434631 None:intergenic 20.0%
!!! TCTTTTTGTTTGATGAATTT+AGG - chr4_1:54434719-54434738 None:intergenic 20.0%
!! TTGAATAAAGTTATGAACTT+TGG + chr4_1:54435047-54435066 Msa0556300:intron 20.0%
!! ATTATAGATTAGTGAATTGA+TGG + chr4_1:54436065-54436084 Msa0556300:intron 20.0%
!! TAATAACATTACATCTTGTT+TGG + chr4_1:54436457-54436476 Msa0556300:intron 20.0%
!!! TTTATGTTATTTCCATTTTG+TGG + chr4_1:54437622-54437641 Msa0556300:three_prime_UTR 20.0%
!! TGTAAGCTGTAAAAAAAAAA+AGG + chr4_1:54437657-54437676 Msa0556300:three_prime_UTR 20.0%
!!! AAAAAAAAAAAGGCCTTTTA+AGG + chr4_1:54437667-54437686 Msa0556300:three_prime_UTR 20.0%
!!! AAAAAAAAAAGGCCTTTTAA+GGG + chr4_1:54437668-54437687 Msa0556300:three_prime_UTR 20.0%
! TAGATGTGAATGTGAAATTA+AGG - chr4_1:54434496-54434515 None:intergenic 25.0%
! ATCAAACAAAAAGATCCAAT+GGG + chr4_1:54434725-54434744 Msa0556300:exon 25.0%
!! TGATGATGATGATATTGAAA+GGG - chr4_1:54434972-54434991 None:intergenic 25.0%
!! ATGATGATGATGATATTGAA+AGG - chr4_1:54434973-54434992 None:intergenic 25.0%
!! ACCCTTTTGAATTTTTTATG+TGG + chr4_1:54435021-54435040 Msa0556300:intron 25.0%
! CCCACATAAAAAATTCAAAA+GGG - chr4_1:54435025-54435044 None:intergenic 25.0%
!! CCCTTTTGAATTTTTTATGT+GGG + chr4_1:54435022-54435041 Msa0556300:intron 25.0%
! ACCCACATAAAAAATTCAAA+AGG - chr4_1:54435026-54435045 None:intergenic 25.0%
!!! TTTGGAGTTTTTGATTCAAT+GGG + chr4_1:54435065-54435084 Msa0556300:intron 25.0%
! TACTACTACAAGATTGAAAA+AGG - chr4_1:54435567-54435586 None:intergenic 25.0%
! TCTTGTAGTAGTAATAGAAA+TGG + chr4_1:54435574-54435593 Msa0556300:CDS 25.0%
!!! AAACTTTACTGTTTTTGTGA+TGG + chr4_1:54435943-54435962 Msa0556300:intron 25.0%
!!! ATGCTAGTCTTAAAAGTTTA+AGG + chr4_1:54436103-54436122 Msa0556300:intron 25.0%
! TGTTTAAATTTAGATCAGCA+AGG + chr4_1:54436207-54436226 Msa0556300:intron 25.0%
! ATATAGACAAGTCAATTTGT+AGG + chr4_1:54436249-54436268 Msa0556300:intron 25.0%
! GTTAAGACCAAAAAACATAT+AGG + chr4_1:54436376-54436395 Msa0556300:intron 25.0%
!! CAATTTTGCATACATAGTTT+TGG + chr4_1:54436427-54436446 Msa0556300:intron 25.0%
! CTTTATTAACCTTAGTTACT+AGG + chr4_1:54436634-54436653 Msa0556300:intron 25.0%
! TTGAGTTATCAAGTTTGAAT+TGG + chr4_1:54436841-54436860 Msa0556300:intron 25.0%
!! ACTTTTATCTGCAAAATTCA+TGG - chr4_1:54437198-54437217 None:intergenic 25.0%
!! TTTGCAGATAAAAGTATACA+TGG + chr4_1:54437203-54437222 Msa0556300:three_prime_UTR 25.0%
!! TTGCAGATAAAAGTATACAT+GGG + chr4_1:54437204-54437223 Msa0556300:three_prime_UTR 25.0%
! AATACATGAATACATAGAAG+AGG + chr4_1:54437253-54437272 Msa0556300:three_prime_UTR 25.0%
!! ATTAGTCCAATGTTATGTTT+TGG + chr4_1:54437451-54437470 Msa0556300:three_prime_UTR 25.0%
!! GTTTGATTTAGAAAGAGTAT+CGG + chr4_1:54437738-54437757 Msa0556300:three_prime_UTR 25.0%
TGAATGTGAAATTAAGGATG+CGG - chr4_1:54434490-54434509 None:intergenic 30.0%
TTCTTGTTGAGGAGTTAAAA+GGG - chr4_1:54434654-54434673 None:intergenic 30.0%
ATTCTTGTTGAGGAGTTAAA+AGG - chr4_1:54434655-54434674 None:intergenic 30.0%
CATCAAACAAAAAGATCCAA+TGG + chr4_1:54434724-54434743 Msa0556300:exon 30.0%
!!! CTTTGGAGTTTTTGATTCAA+TGG + chr4_1:54435064-54435083 Msa0556300:intron 30.0%
!! TTGGAGTTTTTGATTCAATG+GGG + chr4_1:54435066-54435085 Msa0556300:intron 30.0%
! TTTTGTGTATGCAGCAATTT+TGG + chr4_1:54435115-54435134 Msa0556300:intron 30.0%
! TAAAAATTCTGTGCCAGTTT+TGG + chr4_1:54435169-54435188 Msa0556300:CDS 30.0%
TGATCAATCTCTTTCTTCAA+AGG - chr4_1:54435206-54435225 None:intergenic 30.0%
TTGCAACAGTAATCTTAATG+AGG + chr4_1:54435474-54435493 Msa0556300:intron 30.0%
TGCAACAGTAATCTTAATGA+GGG + chr4_1:54435475-54435494 Msa0556300:intron 30.0%
!!! ATGAGGGTTTTGTTTTTGTT+TGG + chr4_1:54435491-54435510 Msa0556300:intron 30.0%
TGTAGTAGTAATAGAAATGG+AGG + chr4_1:54435577-54435596 Msa0556300:CDS 30.0%
!! ACACTCAATTTTGACCAATT+TGG + chr4_1:54436038-54436057 Msa0556300:intron 30.0%
TGATAATCCATAGTTTGGTT+GGG - chr4_1:54436329-54436348 None:intergenic 30.0%
!!! TGATAGGCCTATATGTTTTT+TGG - chr4_1:54436386-54436405 None:intergenic 30.0%
AATTGCAAAGGCAAATCATT+TGG - chr4_1:54436412-54436431 None:intergenic 30.0%
CTATGTATGCAAAATTGCAA+AGG - chr4_1:54436424-54436443 None:intergenic 30.0%
!! ATGAGTTCTCTAATTTGCTT+TGG - chr4_1:54436523-54436542 None:intergenic 30.0%
ACCAAAAACTTACTTGCAAA+TGG - chr4_1:54436582-54436601 None:intergenic 30.0%
TAATAAAGCATGCAGTTAAG+AGG - chr4_1:54436622-54436641 None:intergenic 30.0%
TCATATTGTATGTGCATTCA+TGG + chr4_1:54436783-54436802 Msa0556300:intron 30.0%
AAAAATCTGAGAGTTATAGC+TGG + chr4_1:54437118-54437137 Msa0556300:CDS 30.0%
AAAAGTCATATTCACAGACA+TGG + chr4_1:54437141-54437160 Msa0556300:CDS 30.0%
TTTATCTGCAAAATTCATGG+TGG - chr4_1:54437195-54437214 None:intergenic 30.0%
!!! AGGCTATTGGAATTTTTTGA+AGG + chr4_1:54437273-54437292 Msa0556300:three_prime_UTR 30.0%
GACTAATAATATCTCTTCTG+GGG - chr4_1:54437438-54437457 None:intergenic 30.0%
GGACTAATAATATCTCTTCT+GGG - chr4_1:54437439-54437458 None:intergenic 30.0%
TGGACTAATAATATCTCTTC+TGG - chr4_1:54437440-54437459 None:intergenic 30.0%
TCTTGTTGAGGAGTTAAAAG+GGG - chr4_1:54434653-54434672 None:intergenic 35.0%
GAAGAAGAAGATTCTTGTTG+AGG - chr4_1:54434665-54434684 None:intergenic 35.0%
CATCAACGATCCAAATCTAA+CGG + chr4_1:54434693-54434712 Msa0556300:five_prime_UTR 35.0%
TGAATTTAGGCCGTTAGATT+TGG - chr4_1:54434706-54434725 None:intergenic 35.0%
AAATCTAAACTGAGTTCTGC+AGG - chr4_1:54434761-54434780 None:intergenic 35.0%
! AGGAAATTAGCGATGGTTTT+GGG - chr4_1:54434800-54434819 None:intergenic 35.0%
ATGATAGAGGAAATTAGCGA+TGG - chr4_1:54434807-54434826 None:intergenic 35.0%
ATGGAGTTTGGAGAGTTTAT+CGG - chr4_1:54434852-54434871 None:intergenic 35.0%
!! TTTTGATTCAATGGGGCTAA+TGG + chr4_1:54435073-54435092 Msa0556300:intron 35.0%
TTTGATTCAATGGGGCTAAT+GGG + chr4_1:54435074-54435093 Msa0556300:intron 35.0%
!!! TGCAGCAATTTTGGTTTTGA+AGG + chr4_1:54435124-54435143 Msa0556300:intron 35.0%
!!! AGCAATTTTGGTTTTGAAGG+AGG + chr4_1:54435127-54435146 Msa0556300:intron 35.0%
!!! TTTGGTTTTGAAGGAGGAAT+TGG + chr4_1:54435133-54435152 Msa0556300:CDS 35.0%
GGAATAAACTCTTCCAAAAC+TGG - chr4_1:54435185-54435204 None:intergenic 35.0%
GCAGCAAAGATAAGAAGAAT+TGG + chr4_1:54435276-54435295 Msa0556300:CDS 35.0%
!! TTGTTTGGTGATGACAGAAA+AGG + chr4_1:54435506-54435525 Msa0556300:intron 35.0%
!! TGTTTGGTGATGACAGAAAA+GGG + chr4_1:54435507-54435526 Msa0556300:intron 35.0%
ACAGAAGAATACGGTGAAAA+AGG - chr4_1:54435616-54435635 None:intergenic 35.0%
! ATCGAGAAACAGTTCTAACA+GGG + chr4_1:54435756-54435775 Msa0556300:CDS 35.0%
TCTTATGTAGTTGTGTTGTG+AGG + chr4_1:54436008-54436027 Msa0556300:intron 35.0%
! TTTAGTTCACCAGTGAAGTT+AGG + chr4_1:54436181-54436200 Msa0556300:intron 35.0%
TTAGTTCACCAGTGAAGTTA+GGG + chr4_1:54436182-54436201 Msa0556300:intron 35.0%
TAAACAATCCCTAACTTCAC+TGG - chr4_1:54436193-54436212 None:intergenic 35.0%
TCTATCCCGAATTCAAATCT+TGG + chr4_1:54436272-54436291 Msa0556300:intron 35.0%
AATCTGATCAAGGATTGTTC+CGG - chr4_1:54436298-54436317 None:intergenic 35.0%
GGAGGTTATTAATCTGATCA+AGG - chr4_1:54436308-54436327 None:intergenic 35.0%
CTGATAATCCATAGTTTGGT+TGG - chr4_1:54436330-54436349 None:intergenic 35.0%
!!! GTTTTTTGGTCTTAACCTTC+CGG - chr4_1:54436372-54436391 None:intergenic 35.0%
GCAAATCATTTGGACTTGAT+AGG - chr4_1:54436402-54436421 None:intergenic 35.0%
TCTTCACTTCATCATTAGTC+AGG - chr4_1:54436558-54436577 None:intergenic 35.0%
! GCCATTTGCAAGTAAGTTTT+TGG + chr4_1:54436578-54436597 Msa0556300:intron 35.0%
CATCAAACACCTAGTAACTA+AGG - chr4_1:54436646-54436665 None:intergenic 35.0%
AGTTACTAGGTGTTTGATGA+TGG + chr4_1:54436647-54436666 Msa0556300:intron 35.0%
! AGGCATTTAGGATTGAGATT+TGG + chr4_1:54436818-54436837 Msa0556300:intron 35.0%
TTGTAGTTCTTGGAGGTTTA+TGG + chr4_1:54436953-54436972 Msa0556300:CDS 35.0%
ATTCACAGACATGGAAAAGT+TGG + chr4_1:54437150-54437169 Msa0556300:CDS 35.0%
!! ACATGGAAAAGTTGGTGTAT+AGG + chr4_1:54437158-54437177 Msa0556300:CDS 35.0%
! CAGATAAAAGTATACATGGG+AGG + chr4_1:54437207-54437226 Msa0556300:three_prime_UTR 35.0%
GAATACATAGAAGAGGCTAT+TGG + chr4_1:54437260-54437279 Msa0556300:three_prime_UTR 35.0%
TGATTCTTATCGTGGAATGT+AGG - chr4_1:54437359-54437378 None:intergenic 35.0%
TACATTCCACGATAAGAATC+AGG + chr4_1:54437358-54437377 Msa0556300:three_prime_UTR 35.0%
AAATGACCTGATTCTTATCG+TGG - chr4_1:54437367-54437386 None:intergenic 35.0%
!! AGTCCAATGTTATGTTTTGG+TGG + chr4_1:54437454-54437473 Msa0556300:three_prime_UTR 35.0%
CATCCACCAAAACATAACAT+TGG - chr4_1:54437460-54437479 None:intergenic 35.0%
!! CATCTCTGAAATTTGTGAAG+TGG - chr4_1:54437516-54437535 None:intergenic 35.0%
! TTTTCTCAAAGACACTGAAC+AGG + chr4_1:54437594-54437613 Msa0556300:three_prime_UTR 35.0%
!! GTTATTTCCATTTTGTGGTC+TGG + chr4_1:54437627-54437646 Msa0556300:three_prime_UTR 35.0%
TGACAGAATTTGCCCTTAAA+AGG - chr4_1:54437683-54437702 None:intergenic 35.0%
TCTTTCCTTTCTGCATTGTA+GGG + chr4_1:54437704-54437723 Msa0556300:three_prime_UTR 35.0%
!! AGAAGATAATGGATCCTCTA+CGG - chr4_1:54437792-54437811 None:intergenic 35.0%
TCACAAGTGATAGATAGAGT+AGG - chr4_1:54437821-54437840 None:intergenic 35.0%
!!! ATGGTTTTGGGAACGAAAGT+TGG - chr4_1:54434788-54434807 None:intergenic 40.0%
! GAGGAAATTAGCGATGGTTT+TGG - chr4_1:54434801-54434820 None:intergenic 40.0%
GGATTGTGGAAAGATGATAG+AGG - chr4_1:54434820-54434839 None:intergenic 40.0%
AGAGTTTATCGGAGGTTGTT+TGG - chr4_1:54434841-54434860 None:intergenic 40.0%
AGAGAGGAAATCATGGAGTT+TGG - chr4_1:54434864-54434883 None:intergenic 40.0%
AATACGAACCGTCGTTTAAG+AGG - chr4_1:54434949-54434968 None:intergenic 40.0%
GGATGAGTTCTGTTCAGTTA+TGG + chr4_1:54435297-54435316 Msa0556300:CDS 40.0%
GTCACAGGAACAGAAGAATA+CGG - chr4_1:54435625-54435644 None:intergenic 40.0%
GATAATGTCACTGTTGTCAC+AGG - chr4_1:54435640-54435659 None:intergenic 40.0%
ATCAGCACCTAAAGAAGAGA+AGG + chr4_1:54435657-54435676 Msa0556300:CDS 40.0%
GGTTCTTCCTTCTCTTCTTT+AGG - chr4_1:54435667-54435686 None:intergenic 40.0%
! GATCGAGAAACAGTTCTAAC+AGG + chr4_1:54435755-54435774 Msa0556300:CDS 40.0%
GCAACGCATTAACAAATCTC+CGG - chr4_1:54435891-54435910 None:intergenic 40.0%
! TAGTGAATTGATGGATGTGC+TGG + chr4_1:54436074-54436093 Msa0556300:intron 40.0%
AGATCAGCAAGGAGACAAAT+AGG + chr4_1:54436218-54436237 Msa0556300:intron 40.0%
TTCCGGCCAAGATTTGAATT+CGG - chr4_1:54436281-54436300 None:intergenic 40.0%
ATAACCTCCCAACCAAACTA+TGG + chr4_1:54436319-54436338 Msa0556300:intron 40.0%
TAATCCATAGTTTGGTTGGG+AGG - chr4_1:54436326-54436345 None:intergenic 40.0%
GGGACTGATAATCCATAGTT+TGG - chr4_1:54436334-54436353 None:intergenic 40.0%
! AGAGAACTCATGCAAGTTGA+TGG + chr4_1:54436532-54436551 Msa0556300:CDS 40.0%
ATTCATGGTGTCTGGTTATG+AGG + chr4_1:54436798-54436817 Msa0556300:intron 40.0%
! TGTCTGGTTATGAGGCATTT+AGG + chr4_1:54436806-54436825 Msa0556300:intron 40.0%
!! AGAAATACCGACTTCATACG+CGG + chr4_1:54436896-54436915 Msa0556300:intron 40.0%
GATCAGTCTGTTGTAGTTCT+TGG + chr4_1:54436943-54436962 Msa0556300:CDS 40.0%
GAGTCGTTGTATTGTTCTTG+AGG - chr4_1:54436982-54437001 None:intergenic 40.0%
CAATACAACGACTCTTCCAA+AGG + chr4_1:54436988-54437007 Msa0556300:CDS 40.0%
! GCTAAATGAAGTGGACTTTG+TGG - chr4_1:54437036-54437055 None:intergenic 40.0%
AGTCCACTTCATTTAGCTAC+AGG + chr4_1:54437039-54437058 Msa0556300:CDS 40.0%
GATCCTGTAGCTAAATGAAG+TGG - chr4_1:54437045-54437064 None:intergenic 40.0%
TTCATTTAGCTACAGGATCC+CGG + chr4_1:54437046-54437065 Msa0556300:CDS 40.0%
! CTTTGAGAAAAAACCACGAC+AGG - chr4_1:54437585-54437604 None:intergenic 40.0%
CTCTTTCCTTTCTGCATTGT+AGG + chr4_1:54437703-54437722 Msa0556300:three_prime_UTR 40.0%
TACAACCCTACAATGCAGAA+AGG - chr4_1:54437712-54437731 None:intergenic 40.0%
TTTCTGCATTGTAGGGTTGT+AGG + chr4_1:54437711-54437730 Msa0556300:three_prime_UTR 40.0%
TTCTGCATTGTAGGGTTGTA+GGG + chr4_1:54437712-54437731 Msa0556300:three_prime_UTR 40.0%
!! AGAGTATCGGTTCTCATAGT+AGG + chr4_1:54437751-54437770 Msa0556300:three_prime_UTR 40.0%
GTAGGACTAGGAGAAGATAA+TGG - chr4_1:54437803-54437822 None:intergenic 40.0%
GTGATAGATAGAGTAGGACT+AGG - chr4_1:54437815-54437834 None:intergenic 40.0%
AGAGAAAGCAACGACGAATG+AGG - chr4_1:54434409-54434428 None:intergenic 45.0%
ATCGGAGGTTGTTTGGATTG+TGG - chr4_1:54434834-54434853 None:intergenic 45.0%
GAGTTTGGAGAGTTTATCGG+AGG - chr4_1:54434849-54434868 None:intergenic 45.0%
! TTTAAGAGGAGCATGGAGAG+AGG - chr4_1:54434935-54434954 None:intergenic 45.0%
TCCATGCTCCTCTTAAACGA+CGG + chr4_1:54434938-54434957 Msa0556300:CDS 45.0%
!! TTGGTGATGACAGAAAAGGG+AGG + chr4_1:54435510-54435529 Msa0556300:intron 45.0%
TGACAGAAAAGGGAGGAAGA+AGG + chr4_1:54435517-54435536 Msa0556300:intron 45.0%
ACAATTTGTGACTGCAGCTG+AGG + chr4_1:54435540-54435559 Msa0556300:CDS 45.0%
AAGAGAAGGAAGAACCTGCA+AGG + chr4_1:54435671-54435690 Msa0556300:CDS 45.0%
AGGAAGAACCTGCAAGGAAT+AGG + chr4_1:54435677-54435696 Msa0556300:CDS 45.0%
AAAGAGAGCCTATTCCTTGC+AGG - chr4_1:54435688-54435707 None:intergenic 45.0%
AAGAGTCTGAGGGAAGGATT+TGG + chr4_1:54435724-54435743 Msa0556300:CDS 45.0%
TGTTTCCTAGAAGTCTGCTG+CGG - chr4_1:54435841-54435860 None:intergenic 45.0%
! AGCAGACTTCTAGGAAACAG+AGG + chr4_1:54435842-54435861 Msa0556300:CDS 45.0%
ACAAATCTCCGGTGCAATTC+CGG - chr4_1:54435880-54435899 None:intergenic 45.0%
GTTAATGCGTTGCAGAAGCT+TGG + chr4_1:54435898-54435917 Msa0556300:CDS 45.0%
TCCCGAATTCAAATCTTGGC+CGG + chr4_1:54436276-54436295 Msa0556300:intron 45.0%
TCCGGCCAAGATTTGAATTC+GGG - chr4_1:54436280-54436299 None:intergenic 45.0%
TCTTAACCTTCCGGTTCCTA+GGG - chr4_1:54436363-54436382 None:intergenic 45.0%
GTATGTGCATTCATGGTGTC+TGG + chr4_1:54436790-54436809 Msa0556300:intron 45.0%
CAGTCTGTTGTAGTTCTTGG+AGG + chr4_1:54436946-54436965 Msa0556300:CDS 45.0%
CTCTTCCAAAGGTAGCAGTA+CGG + chr4_1:54436999-54437018 Msa0556300:CDS 45.0%
TCATTTAGCTACAGGATCCC+GGG + chr4_1:54437047-54437066 Msa0556300:CDS 45.0%
TCCATGCTATCACCTTCAGT+TGG - chr4_1:54437084-54437103 None:intergenic 45.0%
! ACCTTTGTGTTTTCCTGTCG+TGG + chr4_1:54437569-54437588 Msa0556300:three_prime_UTR 45.0%
ACCACGACAGGAAAACACAA+AGG - chr4_1:54437573-54437592 None:intergenic 45.0%
ATGAGAGCCAGACCACAAAA+TGG - chr4_1:54437637-54437656 None:intergenic 45.0%
! TAATGGATCCTCTACGGTTG+CGG - chr4_1:54437786-54437805 None:intergenic 45.0%
GCAGGAACAACACAACCCAT+TGG - chr4_1:54434743-54434762 None:intergenic 50.0%
CGAGTCGAGAGAGGAAATCA+TGG - chr4_1:54434871-54434890 None:intergenic 50.0%
GTTCATCTTCGAGTCGAGAG+AGG - chr4_1:54434880-54434899 None:intergenic 50.0%
ACCGTCGTTTAAGAGGAGCA+TGG - chr4_1:54434942-54434961 None:intergenic 50.0%
! GCTCTCTTTTCTGACTCCTG+AGG + chr4_1:54435699-54435718 Msa0556300:CDS 50.0%
TCCTGAGGTGAAGAGTCTGA+GGG + chr4_1:54435714-54435733 Msa0556300:CDS 50.0%
! TGAGGGAAGGATTTGGTTCG+AGG + chr4_1:54435731-54435750 Msa0556300:CDS 50.0%
! GAGGGAAGGATTTGGTTCGA+GGG + chr4_1:54435732-54435751 Msa0556300:CDS 50.0%
! AGGGAAGGATTTGGTTCGAG+GGG + chr4_1:54435733-54435752 Msa0556300:CDS 50.0%
CTAGGAAACAGAGGAGATGC+TGG + chr4_1:54435851-54435870 Msa0556300:CDS 50.0%
AATGCGTTGCAGAAGCTTGG+AGG + chr4_1:54435901-54435920 Msa0556300:CDS 50.0%
CAGAAGCTTGGAGGTTCTCA+AGG + chr4_1:54435910-54435929 Msa0556300:CDS 50.0%
TATCAGTCCCTCCTTTCCCT+AGG + chr4_1:54436344-54436363 Msa0556300:intron 50.0%
GTCTTAACCTTCCGGTTCCT+AGG - chr4_1:54436364-54436383 None:intergenic 50.0%
GAACTCTCCGCGTATGAAGT+CGG - chr4_1:54436906-54436925 None:intergenic 50.0%
TGATCAGTACCACTTGCAGC+TGG - chr4_1:54436928-54436947 None:intergenic 50.0%
CATTTAGCTACAGGATCCCG+GGG + chr4_1:54437048-54437067 Msa0556300:CDS 50.0%
GCCAACTGAAGGTGATAGCA+TGG + chr4_1:54437080-54437099 Msa0556300:CDS 50.0%
ACTTCATACCGCAACCGTAG+AGG + chr4_1:54437775-54437794 Msa0556300:three_prime_UTR 50.0%
CTCCTGAGGTGAAGAGTCTG+AGG + chr4_1:54435713-54435732 Msa0556300:CDS 55.0%
TCCCTCAGACTCTTCACCTC+AGG - chr4_1:54435718-54435737 None:intergenic 55.0%
GAGGTGAAGAGTCTGAGGGA+AGG + chr4_1:54435718-54435737 Msa0556300:CDS 55.0%
TTCAGCCGCAGCAGACTTCT+AGG + chr4_1:54435833-54435852 Msa0556300:CDS 55.0%
ACAGAGGAGATGCTGGTCAC+CGG + chr4_1:54435858-54435877 Msa0556300:CDS 55.0%
TCCCTCCTTTCCCTAGGAAC+CGG + chr4_1:54436350-54436369 Msa0556300:intron 55.0%
TCCGGTTCCTAGGGAAAGGA+GGG - chr4_1:54436354-54436373 None:intergenic 55.0%
TTCCGGTTCCTAGGGAAAGG+AGG - chr4_1:54436355-54436374 None:intergenic 55.0%
TCCTTTCCCTAGGAACCGGA+AGG + chr4_1:54436354-54436373 Msa0556300:intron 55.0%
ACCTTCCGGTTCCTAGGGAA+AGG - chr4_1:54436358-54436377 None:intergenic 55.0%
AGACGCCGTACTGCTACCTT+TGG - chr4_1:54437007-54437026 None:intergenic 55.0%
AAAGGTAGCAGTACGGCGTC+TGG + chr4_1:54437006-54437025 Msa0556300:CDS 55.0%
ATACTCGGCTGCACGGTTGG+AGG - chr4_1:54435799-54435818 None:intergenic 60.0%
AGCACGCATACTCGGCTGCA+CGG - chr4_1:54435806-54435825 None:intergenic 60.0%
GAAGTCTGCTGCGGCTGAAG+CGG - chr4_1:54435832-54435851 None:intergenic 60.0%
GCTGGTCACCGGAATTGCAC+CGG + chr4_1:54435869-54435888 Msa0556300:CDS 60.0%
CGGAGAGTTCCAGCTGCAAG+TGG + chr4_1:54436916-54436935 Msa0556300:CDS 60.0%
TTAGCTACAGGATCCCGGGG+AGG + chr4_1:54437051-54437070 Msa0556300:CDS 60.0%
TAGCTACAGGATCCCGGGGA+GGG + chr4_1:54437052-54437071 Msa0556300:CDS 60.0%
GGAGGGACATCGCCAACTGA+AGG + chr4_1:54437069-54437088 Msa0556300:CDS 60.0%
CGCATACTCGGCTGCACGGT+TGG - chr4_1:54435802-54435821 None:intergenic 65.0%
AGCGGCGCAGCACGCATACT+CGG - chr4_1:54435814-54435833 None:intergenic 65.0%
AGTTGGCGATGTCCCTCCCC+GGG - chr4_1:54437067-54437086 None:intergenic 65.0%
CAGTTGGCGATGTCCCTCCC+CGG - chr4_1:54437068-54437087 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 54434399 54437847 54434399 ID=Msa0556300;Name=Msa0556300
chr4_1 mRNA 54434399 54437847 54434399 ID=Msa0556300-mRNA-1;Parent=Msa0556300;Name=Msa0556300-mRNA-1;_AED=0.11;_eAED=0.11;_QI=344|0.75|0.8|1|1|1|5|668|420
chr4_1 exon 54434399 54434959 54434399 ID=Msa0556300-mRNA-1:exon:16015;Parent=Msa0556300-mRNA-1
chr4_1 exon 54435129 54435406 54435129 ID=Msa0556300-mRNA-1:exon:16016;Parent=Msa0556300-mRNA-1
chr4_1 exon 54435523 54435931 54435523 ID=Msa0556300-mRNA-1:exon:16017;Parent=Msa0556300-mRNA-1
chr4_1 exon 54436512 54436588 54436512 ID=Msa0556300-mRNA-1:exon:16018;Parent=Msa0556300-mRNA-1
chr4_1 exon 54436898 54437847 54436898 ID=Msa0556300-mRNA-1:exon:16019;Parent=Msa0556300-mRNA-1
chr4_1 five_prime_UTR 54434399 54434742 54434399 ID=Msa0556300-mRNA-1:five_prime_utr;Parent=Msa0556300-mRNA-1
chr4_1 CDS 54434743 54434959 54434743 ID=Msa0556300-mRNA-1:cds;Parent=Msa0556300-mRNA-1
chr4_1 CDS 54435129 54435406 54435129 ID=Msa0556300-mRNA-1:cds;Parent=Msa0556300-mRNA-1
chr4_1 CDS 54435523 54435931 54435523 ID=Msa0556300-mRNA-1:cds;Parent=Msa0556300-mRNA-1
chr4_1 CDS 54436512 54436588 54436512 ID=Msa0556300-mRNA-1:cds;Parent=Msa0556300-mRNA-1
chr4_1 CDS 54436898 54437179 54436898 ID=Msa0556300-mRNA-1:cds;Parent=Msa0556300-mRNA-1
chr4_1 three_prime_UTR 54437180 54437847 54437180 ID=Msa0556300-mRNA-1:three_prime_utr;Parent=Msa0556300-mRNA-1
Gene Sequence

>Msa0556300

ATGGGTTGTGTTGTTCCTGCAGAACTCAGTTTAGATTTAAGACCAACTTTCGTTCCCAAAACCATCGCTAATTTCCTCTATCATCTTTCCACAATCCAAACAACCTCCGATAAACTCTCCAAACTCCATGATTTCCTCTCTCGACTCGAAGATGAACTCAATAAGATCGATGCTTTCAAACGTGAACTACCTCTCTCCATGCTCCTCTTAAACGACGCAATTTTGGTTTTGAAGGAGGAATTGGAAAAATGTAGAAGTAAAAATTCTGTGCCAGTTTTGGAAGAGTTTATTCCTTTGAAGAAAGAGATTGATCAGAGTGAAGAGAATAATAATAATGATAGAGACAAGAACAACAACAATGAATGCAGCAAAGATAAGAAGAATTGGATGAGTTCTGTTCAGTTATGGAATAACAACACAACAACTTCTAATAATAATGTCTCTGATCATCATCATAAACATAACAATCATAATCTTAATAAATTAGAAACAAAGAAAAGGGAGGAAGAAGGACAATTTGTGACTGCAGCTGAGGACCTTTTTCAATCTTGTAGTAGTAATAGAAATGGAGGAAGTGCTTTTATGCCTTTTTCACCGTATTCTTCTGTTCCTGTGACAACAGTGACATTATCAGCACCTAAAGAAGAGAAGGAAGAACCTGCAAGGAATAGGCTCTCTTTTCTGACTCCTGAGGTGAAGAGTCTGAGGGAAGGATTTGGTTCGAGGGGATCGAGAAACAGTTCTAACAGGGCAGTTTCGTCGTCGTCTCCTCCAACCGTGCAGCCGAGTATGCGTGCTGCGCCGCTTCAGCCGCAGCAGACTTCTAGGAAACAGAGGAGATGCTGGTCACCGGAATTGCACCGGAGATTTGTTAATGCGTTGCAGAAGCTTGGAGGTTCTCAAGCAGCAACTCCAAAGCAAATTAGAGAACTCATGCAAGTTGATGGCCTGACTAATGATGAAGTGAAGAGCCATTTGCAAAAATACCGACTTCATACGCGGAGAGTTCCAGCTGCAAGTGGTACTGATCAGTCTGTTGTAGTTCTTGGAGGTTTATGGATGCCTCAAGAACAATACAACGACTCTTCCAAAGGTAGCAGTACGGCGTCTGGTTCTCCACAAAGTCCACTTCATTTAGCTACAGGATCCCGGGGAGGGACATCGCCAACTGAAGGTGATAGCATGGACGATGACGATGATGAAAAATCTGAGAGTTATAGCTGGAAAAGTCATATTCACAGACATGGAAAAGTTGGTGTATAG
Protein sequence

>Msa0556300

MGCVVPAELSLDLRPTFVPKTIANFLYHLSTIQTTSDKLSKLHDFLSRLEDELNKIDAFKRELPLSMLLLNDAILVLKEELEKCRSKNSVPVLEEFIPLKKEIDQSEENNNNDRDKNNNNECSKDKKNWMSSVQLWNNNTTTSNNNVSDHHHKHNNHNLNKLETKKREEEGQFVTAAEDLFQSCSSNRNGGSAFMPFSPYSSVPVTTVTLSAPKEEKEEPARNRLSFLTPEVKSLREGFGSRGSRNSSNRAVSSSSPPTVQPSMRAAPLQPQQTSRKQRRCWSPELHRRFVNALQKLGGSQAATPKQIRELMQVDGLTNDEVKSHLQKYRLHTRRVPAASGTDQSVVVLGGLWMPQEQYNDSSKGSSTASGSPQSPLHLATGSRGGTSPTEGDSMDDDDDEKSESYSWKSHIHRHGKVGV*