AlfalfaGEDB | Msa0694530

M. sativa cultivar zhongmu-4 / Msa0694530

KEGG: -
GO: GO:0000156 GO:0000160 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0009653 GO:0009719 GO:0009723 GO:0009725 GO:0009735 GO:0009736 GO:0009755 GO:0009873 GO:0009888 GO:0009889 GO:0009987 GO:0010015 GO:0010033 GO:0010073 GO:0010075 GO:0010082 GO:0010119 GO:0010150 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022622 GO:0023052 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032870 GO:0035556 GO:0040008 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048364 GO:0048366 GO:0048367 GO:0048507 GO:0048509 GO:0048580 GO:0048638 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051239 GO:0051252 GO:0051716 GO:0060089 GO:0060255 GO:0065007 GO:0070887 GO:0071310 GO:0071368 GO:0071369 GO:0071495 GO:0080050 GO:0080090 GO:0080113 GO:0090693 GO:0099402 GO:0140110 GO:1903506 GO:1905392 GO:2000026 GO:2000112 GO:2000241 GO:2000280 GO:2001141
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0694530	XP_013456887.1	95.714	420	15	1	1	420	1	417	0.0	825

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0694530	sp\|Q9ZQ85\|EFM_ARATH	40.533	375	158	10	7	368	5	327	2.62e-63	213

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0694530	A0A072UPI8	95.714	420	15	1	1	420	1	417	0.0	825

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TFs/TRs:

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Gene ID	Type	Classification
Msa0694530	TF	GARP-G2-like

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0556300	Msa0694530	0.946783	2.385271e-105	-8.615850e-47
Msa0651980	Msa0694530	0.811712	6.102384e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0694530	MtrunA17_Chr4g0045361	95.714	420	15	1	1	420	1	417	0.0	825
Msa0694530	MtrunA17_Chr4g0062921	39.788	377	158	14	7	367	5	328	2.59e-65	215
Msa0694530	MtrunA17_Chr5g0402631	39.216	408	155	11	8	410	27	346	3.62e-61	201
Msa0694530	MtrunA17_Chr1g0198601	34.903	361	145	12	42	371	8	309	4.73e-46	163
Msa0694530	MtrunA17_Chr5g0422621	35.942	345	142	12	41	371	16	295	1.52e-44	158
Msa0694530	MtrunA17_Chr1g0175081	47.059	68	36	0	274	341	19	86	2.86e-15	71.2
Msa0694530	MtrunA17_Chr7g0255711	50.746	67	32	1	270	336	84	149	3.32e-15	75.9
Msa0694530	MtrunA17_Chr7g0243081	40.698	86	51	0	245	330	39	124	3.39e-14	73.6
Msa0694530	MtrunA17_Chr2g0288601	50.769	65	32	0	263	327	57	121	4.66e-14	68.9
Msa0694530	MtrunA17_Chr7g0261911	53.030	66	31	0	277	342	262	327	5.85e-14	73.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0694530	AT2G03500.1	40.533	375	158	10	7	368	5	327	2.66e-64	213
Msa0694530	AT4G37180.1	35.561	374	157	10	9	377	12	306	1.88e-50	174
Msa0694530	AT4G37180.2	35.372	376	162	12	9	377	12	313	2.91e-48	169
Msa0694530	AT4G37180.3	35.737	319	135	8	9	327	12	260	1.43e-43	154
Msa0694530	AT1G49560.1	53.797	158	57	5	261	406	172	325	1.37e-38	142
Msa0694530	AT1G68670.1	69.892	93	20	1	274	358	212	304	7.85e-36	135
Msa0694530	AT1G68670.1	35.000	120	62	4	36	139	8	127	1.21e-11	66.2
Msa0694530	AT1G13300.1	70.787	89	21	2	276	359	180	268	4.53e-34	130
Msa0694530	AT1G25550.1	54.622	119	47	2	274	385	206	324	2.31e-33	128
Msa0694530	AT1G25550.1	33.588	131	67	2	36	146	12	142	9.40e-15	75.5

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Find 136 sgRNAs with CRISPR-Local

Find 231 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTCATATTCACAGACA+TGG	0.616407	4_4:+63852968	Msa0694530:CDS
AAAGAGAGCCTATTCCTTAC+AGG	0.270823	4_4:-63851512	None:intergenic
AAAGGTAGCAGTACGGCGTC+TGG	0.473278	4_4:+63852833	Msa0694530:CDS
AAATCTAAACTGAGTTCTGC+AGG	0.491660	4_4:-63850579	None:intergenic
AAATGACCTGATTCTTATCG+TGG	0.563659	4_4:-63853191	None:intergenic
AAGAGAAGGAAGAACCTGTA+AGG	0.506152	4_4:+63851498	Msa0694530:CDS
AAGAGTCTGAGGGAAGGATT+TGG	0.407195	4_4:+63851551	Msa0694530:CDS
AATAATGTAATAAGAAGAAG+TGG	0.434366	4_4:-63850333	None:intergenic
AATACATGAATACATAGAAG+AGG	0.594019	4_4:+63853080	Msa0694530:three_prime_UTR
AATACGAACCGTCGTTTAAG+AGG	0.421808	4_4:-63850767	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGAATGTGAAATTAAGGATG+CGG	-	chr4_4:63850305-63850324	None:intergenic	30.0%
TTCTTGTTGAGGAGTTAAAA+GGG	-	chr4_4:63850469-63850488	None:intergenic	30.0%
ATTCTTGTTGAGGAGTTAAA+AGG	-	chr4_4:63850470-63850489	None:intergenic	30.0%
CATCAAACAAAAAGATCCAA+TGG	+	chr4_4:63850545-63850564	Msa0694530:exon	30.0%
TGATCAATCTCTTTCTTCAA+AGG	-	chr4_4:63851033-63851052	None:intergenic	30.0%
TTCCAACAGTAATCTTAATG+AGG	+	chr4_4:63851301-63851320	Msa0694530:intron	30.0%
TCCAACAGTAATCTTAATGA+GGG	+	chr4_4:63851302-63851321	Msa0694530:intron	30.0%
TGTAGTAGTAATAGAAATGG+AGG	+	chr4_4:63851404-63851423	Msa0694530:CDS	30.0%
TGATAATCCATAGTTTGGTT+GGG	-	chr4_4:63852156-63852175	None:intergenic	30.0%
AATTGCAAAGGCAAATCATT+TGG	-	chr4_4:63852239-63852258	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_4	gene	63850215	63853673	63850215	ID=Msa0694530;Name=Msa0694530
chr4_4	mRNA	63850215	63853673	63850215	ID=Msa0694530-mRNA-1;Parent=Msa0694530;Name=Msa0694530-mRNA-1;_AED=0.12;_eAED=0.12;_QI=349\|0.75\|0.8\|1\|1\|1\|5\|667\|420
chr4_4	exon	63850215	63850780	63850215	ID=Msa0694530-mRNA-1:exon:18123;Parent=Msa0694530-mRNA-1
chr4_4	exon	63850956	63851233	63850956	ID=Msa0694530-mRNA-1:exon:18124;Parent=Msa0694530-mRNA-1
chr4_4	exon	63851350	63851758	63851350	ID=Msa0694530-mRNA-1:exon:18125;Parent=Msa0694530-mRNA-1
chr4_4	exon	63852339	63852415	63852339	ID=Msa0694530-mRNA-1:exon:18126;Parent=Msa0694530-mRNA-1
chr4_4	exon	63852725	63853673	63852725	ID=Msa0694530-mRNA-1:exon:18127;Parent=Msa0694530-mRNA-1
chr4_4	five_prime_UTR	63850215	63850563	63850215	ID=Msa0694530-mRNA-1:five_prime_utr;Parent=Msa0694530-mRNA-1
chr4_4	CDS	63850564	63850780	63850564	ID=Msa0694530-mRNA-1:cds;Parent=Msa0694530-mRNA-1
chr4_4	CDS	63850956	63851233	63850956	ID=Msa0694530-mRNA-1:cds;Parent=Msa0694530-mRNA-1
chr4_4	CDS	63851350	63851758	63851350	ID=Msa0694530-mRNA-1:cds;Parent=Msa0694530-mRNA-1
chr4_4	CDS	63852339	63852415	63852339	ID=Msa0694530-mRNA-1:cds;Parent=Msa0694530-mRNA-1
chr4_4	CDS	63852725	63853006	63852725	ID=Msa0694530-mRNA-1:cds;Parent=Msa0694530-mRNA-1
chr4_4	three_prime_UTR	63853007	63853673	63853007	ID=Msa0694530-mRNA-1:three_prime_utr;Parent=Msa0694530-mRNA-1

Gene Sequence

>Msa0694530

ATGGGTTGTGTTGTTCCTGCAGAACTCAGTTTAGATTTAAGACCAACTTTCGTTCCCAAAACCATCGCTAATTTCCTCTATCATCTTTCCACAATCCAAACAACCTCCGATAAACTCTCCAAACTCCATGATTTCCTCTCTCGACTCGAAGATGAACTCAATAAGATCGATGCTTTCAAACGTGAACTACCTCTCTCCATGCTCCTCTTAAACGACGCAATTTTGGTTTTGAAGGAGGAATTGGAAAAATGTAGAAGTAAAAATTCTGTGCCAGTTTTGGAAGAGTTTATTCCTTTGAAGAAAGAGATTGATCAGAGTGAAGAGAATAATAATAATGATAGAGACAAGAACAACAACAATGAATGCAGCAAAGATAAGAAGAATTGGATGAGTTCTGTTCAGTTATGGAATAACAACACAACAACTTCTAATAATAATGTCTCTGATCATCATCATAAACATAACAATCATAATCTTAATAAATTAGAAACAAAGAAAAGGGAGGAAGAAGGACAATTTGTGACTGCAGCTGAGGACCTTTTTCAATCTTGTAGTAGTAATAGAAATGGAGGAAGTGCTTTTATGCCTTTTTCACCGTATTCTTCTGTTCCTGTGACAACAGTGACATTATCAGCACCTAAAGAAGAGAAGGAAGAACCTGTAAGGAATAGGCTCTCTTTTCTGACTCCTGAGGTGAAGAGTCTGAGGGAAGGATTTGGTTCGAGGGGATCGAGAAACAGTTCTAACAGGGCAGTTTCGTCGTCGTCTCCTCCAACCGTGCAGCCGAGTATGCGTGCTGCGCCACTTCAGCCGCAGCAGACTTCTAGGAAACAGAGGAGATGCTGGTCACCGGAATTGCACCGGAGATTTGTTAATGCGTTGCAGAAGCTTGGAGGTTCTCAAGCAGCAACTCCAAAGCAAATTAGAGAACTCATGCAAGTTGATGGCCTGACTAATGATGAAGTGAAGAGCCATTTGCAAAAATACCGACTTCATACGCGGAGAGTTCCAGCTGCAAGTGGTACTGATCAGTCTGTTGTAGTTCTTGGAGGTTTATGGATGCCTCAAGAACAATACAACGACTCTTCCAAAGGTAGCAGTACGGCGTCTGGTTCTCCACAAAGTCCACTTCATTTAGCTACAGGATCCCGGGGAGGGACATCGCCAACTGAAGGTGATAGCATGGACGATGACGATGATGAAAAATCTGAGAGTTATAGCTGGAAAAGTCATATTCACAGACATGGAAAAGTTGGTGTATAG

Protein sequence

>Msa0694530

MGCVVPAELSLDLRPTFVPKTIANFLYHLSTIQTTSDKLSKLHDFLSRLEDELNKIDAFKRELPLSMLLLNDAILVLKEELEKCRSKNSVPVLEEFIPLKKEIDQSEENNNNDRDKNNNNECSKDKKNWMSSVQLWNNNTTTSNNNVSDHHHKHNNHNLNKLETKKREEEGQFVTAAEDLFQSCSSNRNGGSAFMPFSPYSSVPVTTVTLSAPKEEKEEPVRNRLSFLTPEVKSLREGFGSRGSRNSSNRAVSSSSPPTVQPSMRAAPLQPQQTSRKQRRCWSPELHRRFVNALQKLGGSQAATPKQIRELMQVDGLTNDEVKSHLQKYRLHTRRVPAASGTDQSVVVLGGLWMPQEQYNDSSKGSSTASGSPQSPLHLATGSRGGTSPTEGDSMDDDDDEKSESYSWKSHIHRHGKVGV*