AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0694530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0694530 MtrunA17_Chr4g0045361 95.714 420 15 1 1 420 1 417 0.0 825
Msa0694530 MtrunA17_Chr4g0062921 39.788 377 158 14 7 367 5 328 2.59e-65 215
Msa0694530 MtrunA17_Chr5g0402631 39.216 408 155 11 8 410 27 346 3.62e-61 201
Msa0694530 MtrunA17_Chr1g0198601 34.903 361 145 12 42 371 8 309 4.73e-46 163
Msa0694530 MtrunA17_Chr5g0422621 35.942 345 142 12 41 371 16 295 1.52e-44 158
Msa0694530 MtrunA17_Chr1g0175081 47.059 68 36 0 274 341 19 86 2.86e-15 71.2
Msa0694530 MtrunA17_Chr7g0255711 50.746 67 32 1 270 336 84 149 3.32e-15 75.9
Msa0694530 MtrunA17_Chr7g0243081 40.698 86 51 0 245 330 39 124 3.39e-14 73.6
Msa0694530 MtrunA17_Chr2g0288601 50.769 65 32 0 263 327 57 121 4.66e-14 68.9
Msa0694530 MtrunA17_Chr7g0261911 53.030 66 31 0 277 342 262 327 5.85e-14 73.6
Msa0694530 MtrunA17_Chr5g0417521 55.172 58 26 0 274 331 12 69 7.23e-14 72.4
Msa0694530 MtrunA17_Chr1g0190301 61.111 54 21 0 277 330 175 228 1.14e-13 72.0
Msa0694530 MtrunA17_Chr8g0366281 48.980 98 43 4 251 341 142 239 1.32e-13 71.6
Msa0694530 MtrunA17_Chr7g0254981 49.315 73 36 1 269 341 42 113 1.47e-13 70.9
Msa0694530 MtrunA17_Chr2g0304841 48.052 77 35 3 276 351 212 284 1.66e-13 72.4
Msa0694530 MtrunA17_Chr7g0274471 62.963 54 20 0 277 330 218 271 1.90e-13 71.6
Msa0694530 MtrunA17_Chr1g0149841 50.000 68 33 1 272 339 186 252 2.22e-13 71.6
Msa0694530 MtrunA17_Chr5g0400241 47.826 69 35 1 268 336 302 369 2.27e-13 72.0
Msa0694530 MtrunA17_Chr4g0067981 53.731 67 30 1 274 340 212 277 2.48e-13 72.0
Msa0694530 MtrunA17_Chr4g0031961 47.059 68 35 1 273 340 127 193 3.10e-13 70.1
Msa0694530 MtrunA17_Chr1g0162931 63.265 49 18 0 282 330 23 71 4.19e-13 69.3
Msa0694530 MtrunA17_Chr4g0040481 43.820 89 45 2 273 357 154 241 4.54e-13 70.5
Msa0694530 MtrunA17_Chr2g0294911 50.746 67 32 1 274 340 208 273 7.51e-13 70.5
Msa0694530 MtrunA17_Chr1g0210911 61.224 49 19 0 282 330 27 75 7.78e-13 69.3
Msa0694530 MtrunA17_Chr3g0137101 42.529 87 38 2 276 351 197 282 8.86e-13 70.1
Msa0694530 MtrunA17_Chr2g0291021 60.000 55 22 0 277 331 49 103 9.33e-13 68.9
Msa0694530 MtrunA17_Chr5g0410741 46.465 99 48 2 248 341 187 285 1.37e-12 68.9
Msa0694530 MtrunA17_Chr4g0054981 31.250 128 76 2 209 336 253 368 1.39e-12 69.3
Msa0694530 MtrunA17_Chr2g0321461 53.448 58 27 0 274 331 42 99 2.17e-12 68.2
Msa0694530 MtrunA17_Chr6g0461641 44.000 75 41 1 274 348 39 112 2.97e-12 67.8
Msa0694530 MtrunA17_Chr1g0149831 48.438 64 29 1 276 339 127 186 3.50e-12 65.9
Msa0694530 MtrunA17_Chr1g0161431 47.761 67 34 1 274 340 197 262 3.78e-12 68.2
Msa0694530 MtrunA17_Chr6g0479431 41.935 93 47 2 264 349 184 276 4.17e-12 67.4
Msa0694530 MtrunA17_Chr1g0175091 48.529 68 35 0 274 341 255 322 5.64e-12 67.0
Msa0694530 MtrunA17_Chr4g0074411 51.613 62 29 1 274 335 198 258 1.22e-11 66.6
Msa0694530 MtrunA17_Chr5g0409731 54.098 61 26 1 277 335 25 85 1.28e-11 65.1
Msa0694530 MtrunA17_Chr8g0356501 55.172 58 26 0 274 331 34 91 1.30e-11 65.5
Msa0694530 MtrunA17_Chr4g0042451 51.724 58 28 0 274 331 14 71 1.53e-11 65.5
Msa0694530 MtrunA17_Chr4g0009901 49.180 61 30 1 275 335 166 225 2.13e-11 64.3
Msa0694530 MtrunA17_Chr3g0095611 50.000 56 28 0 275 330 17 72 2.34e-11 63.2
Msa0694530 MtrunA17_Chr1g0197171 56.364 55 24 0 277 331 44 98 2.40e-11 64.3
Msa0694530 MtrunA17_Chr2g0324891 49.180 61 30 1 276 336 160 219 2.58e-11 65.1
Msa0694530 MtrunA17_Chr2g0310011 55.172 58 26 0 274 331 32 89 2.60e-11 64.7
Msa0694530 MtrunA17_Chr3g0109981 49.315 73 36 1 269 340 266 338 3.16e-11 65.1
Msa0694530 MtrunA17_Chr2g0320231 45.455 66 36 0 265 330 85 150 4.82e-11 63.9
Msa0694530 MtrunA17_Chr2g0290991 56.364 55 24 0 277 331 39 93 5.59e-11 63.5
Msa0694530 MtrunA17_Chr7g0258551 50.877 57 28 0 274 330 209 265 5.73e-11 63.9
Msa0694530 MtrunA17_Chr1g0154261 41.975 81 45 1 272 352 121 199 6.83e-11 62.8
Msa0694530 MtrunA17_Chr4g0074401 48.387 62 31 1 274 335 200 260 9.47e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0694530 AT2G03500.1 40.533 375 158 10 7 368 5 327 2.66e-64 213
Msa0694530 AT4G37180.1 35.561 374 157 10 9 377 12 306 1.88e-50 174
Msa0694530 AT4G37180.2 35.372 376 162 12 9 377 12 313 2.91e-48 169
Msa0694530 AT4G37180.3 35.737 319 135 8 9 327 12 260 1.43e-43 154
Msa0694530 AT1G49560.1 53.797 158 57 5 261 406 172 325 1.37e-38 142
Msa0694530 AT1G68670.1 69.892 93 20 1 274 358 212 304 7.85e-36 135
Msa0694530 AT1G68670.1 35.000 120 62 4 36 139 8 127 1.21e-11 66.2
Msa0694530 AT1G13300.1 70.787 89 21 2 276 359 180 268 4.53e-34 130
Msa0694530 AT1G25550.1 54.622 119 47 2 274 385 206 324 2.31e-33 128
Msa0694530 AT1G25550.1 33.588 131 67 2 36 146 12 142 9.40e-15 75.5
Msa0694530 AT3G25790.1 57.292 96 36 1 281 371 200 295 1.75e-30 120
Msa0694530 AT1G49560.2 66.667 81 21 2 251 327 164 242 5.28e-25 103
Msa0694530 AT1G25550.2 83.333 54 9 0 274 327 206 259 1.12e-24 103
Msa0694530 AT1G25550.2 33.588 131 67 2 36 146 12 142 1.73e-14 73.9
Msa0694530 AT3G25790.2 78.723 47 10 0 281 327 200 246 1.71e-19 88.2
Msa0694530 AT5G05090.1 50.746 67 32 1 270 336 74 139 2.92e-15 75.9
Msa0694530 AT3G10760.1 50.704 71 34 1 266 336 94 163 2.92e-15 77.0
Msa0694530 AT2G40970.1 50.746 67 32 1 270 336 98 163 4.01e-15 75.1
Msa0694530 AT2G38300.1 46.053 76 41 0 255 330 32 107 1.50e-14 74.7
Msa0694530 AT5G59570.2 50.000 64 31 1 273 336 137 199 1.93e-14 73.9
Msa0694530 AT5G59570.1 50.000 64 31 1 273 336 137 199 1.93e-14 73.9
Msa0694530 AT3G04450.3 50.685 73 35 1 258 330 220 291 1.61e-13 71.6
Msa0694530 AT2G06020.1 35.652 115 72 1 232 346 41 153 1.90e-13 71.2
Msa0694530 AT3G04450.1 43.333 90 50 1 258 347 220 308 3.39e-13 71.2
Msa0694530 AT3G16857.2 43.137 102 44 2 276 377 236 323 3.58e-13 72.0
Msa0694530 AT3G16857.3 43.137 102 44 2 276 377 166 253 3.60e-13 71.6
Msa0694530 AT5G58080.1 50.794 63 30 1 274 336 191 252 3.65e-13 71.6
Msa0694530 AT3G46640.2 48.438 64 32 1 273 336 140 202 4.13e-13 70.5
Msa0694530 AT3G46640.1 48.438 64 32 1 273 336 140 202 4.13e-13 70.5
Msa0694530 AT3G13040.3 58.929 56 23 0 276 331 240 295 4.18e-13 71.2
Msa0694530 AT3G13040.1 58.929 56 23 0 276 331 240 295 4.18e-13 71.2
Msa0694530 AT3G13040.2 58.929 56 23 0 276 331 240 295 4.18e-13 71.2
Msa0694530 AT3G16857.1 43.137 102 44 2 276 377 236 323 4.24e-13 71.6
Msa0694530 AT3G04450.4 43.333 90 50 1 258 347 180 268 4.39e-13 70.9
Msa0694530 AT3G04450.2 43.333 90 50 1 258 347 180 268 4.39e-13 70.9
Msa0694530 AT4G28610.1 52.239 67 31 1 277 342 225 291 5.22e-13 70.9
Msa0694530 AT2G01760.3 54.839 62 27 1 275 336 133 193 5.43e-13 70.1
Msa0694530 AT3G46640.3 48.438 64 32 1 273 336 140 202 5.69e-13 70.1
Msa0694530 AT2G01760.2 54.839 62 27 1 275 336 226 286 6.22e-13 70.5
Msa0694530 AT2G01760.1 54.839 62 27 1 275 336 198 258 6.84e-13 70.1
Msa0694530 AT2G01060.1 60.000 55 22 0 277 331 15 69 7.68e-13 68.9
Msa0694530 AT5G29000.4 56.140 57 25 0 274 330 228 284 1.41e-12 68.6
Msa0694530 AT5G18240.2 55.172 58 26 0 274 331 42 99 1.70e-12 68.9
Msa0694530 AT5G18240.3 55.172 58 26 0 274 331 42 99 1.70e-12 68.9
Msa0694530 AT5G18240.5 55.172 58 26 0 274 331 42 99 1.93e-12 68.9
Msa0694530 AT2G20400.2 50.000 70 34 1 277 345 231 300 1.95e-12 68.9
Msa0694530 AT2G20400.1 53.125 64 29 1 277 340 231 293 1.97e-12 68.9
Msa0694530 AT5G18240.1 55.172 58 26 0 274 331 42 99 2.05e-12 68.9
Msa0694530 AT5G18240.4 55.172 58 26 0 274 331 42 99 2.05e-12 68.9
Msa0694530 AT3G04030.1 53.448 58 27 0 274 331 42 99 2.32e-12 68.6
Msa0694530 AT3G04030.3 53.448 58 27 0 274 331 42 99 2.77e-12 68.6
Msa0694530 AT5G29000.2 59.259 54 22 0 277 330 231 284 3.80e-12 68.2
Msa0694530 AT5G29000.1 59.259 54 22 0 277 330 188 241 3.97e-12 67.8
Msa0694530 AT5G29000.3 59.259 54 22 0 277 330 188 241 3.97e-12 67.8
Msa0694530 AT2G02060.2 50.000 62 31 0 269 330 21 82 6.25e-12 65.9
Msa0694530 AT2G02060.1 50.000 62 31 0 269 330 21 82 6.25e-12 65.9
Msa0694530 AT5G06800.3 44.156 77 40 2 255 331 173 246 6.89e-12 66.2
Msa0694530 AT1G14600.1 51.613 62 30 0 269 330 15 76 6.91e-12 65.9
Msa0694530 AT5G45580.1 48.529 68 35 0 277 344 7 74 7.12e-12 65.9
Msa0694530 AT5G45580.2 47.826 69 36 0 277 345 23 91 7.85e-12 65.9
Msa0694530 AT2G25180.1 46.154 65 34 1 276 340 194 257 7.94e-12 67.4
Msa0694530 AT1G32240.2 45.455 88 47 1 265 351 200 287 9.19e-12 66.6
Msa0694530 AT3G12730.1 55.172 58 26 0 274 331 20 77 1.06e-11 65.1
Msa0694530 AT1G32240.1 45.455 88 47 1 265 351 200 287 1.10e-11 66.6
Msa0694530 AT5G16560.1 50.000 78 37 2 265 340 206 283 2.35e-11 65.5
Msa0694530 AT1G67710.3 51.724 58 28 0 279 336 121 178 2.90e-11 65.5
Msa0694530 AT1G67710.2 51.724 58 28 0 279 336 130 187 2.93e-11 65.5
Msa0694530 AT1G67710.1 51.724 58 28 0 279 336 195 252 3.80e-11 65.5
Msa0694530 AT5G06800.2 44.156 77 40 2 255 331 173 246 3.96e-11 64.7
Msa0694530 AT1G79430.2 55.172 58 26 0 274 331 31 88 4.29e-11 64.7
Msa0694530 AT3G24120.1 55.172 58 26 0 274 331 38 95 4.57e-11 63.9
Msa0694530 AT5G06800.1 44.156 77 40 2 255 331 173 246 4.73e-11 64.3
Msa0694530 AT4G13640.1 56.364 55 24 0 277 331 37 91 5.02e-11 63.5
Msa0694530 AT3G24120.2 55.172 58 26 0 274 331 38 95 5.04e-11 63.9
Msa0694530 AT4G13640.2 56.364 55 24 0 277 331 37 91 5.30e-11 63.5
Msa0694530 AT2G42660.1 55.102 49 22 0 282 330 55 103 8.73e-11 62.4
Msa0694530 AT2G40260.1 46.154 65 35 0 266 330 71 135 9.20e-11 63.9

Find 136 sgRNAs with CRISPR-Local

Find 231 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTAGTTCTTGGAGGTTTA+TGG 0.186325 4_4:+63852780 Msa0694530:CDS
GTGATGAGATTTATATAAAA+TGG 0.205392 4_4:-63850424 None:intergenic
CTCTTTCCTTTCTGCATTGT+AGG 0.213979 4_4:+63853529 Msa0694530:three_prime_UTR
ATTCTTGTTGAGGAGTTAAA+AGG 0.219449 4_4:-63850467 None:intergenic
GGTTCTTCCTTCTCTTCTTT+AGG 0.234160 4_4:-63851491 None:intergenic
TGACAGAATTTGCCCTTAAA+AGG 0.238192 4_4:-63853506 None:intergenic
AAAGAGAGCCTATTCCTTAC+AGG 0.270823 4_4:-63851512 None:intergenic
TGGACTAATAATATCTCTTC+TGG 0.271849 4_4:-63853264 None:intergenic
TTTCTGCATTGTAGGGTTGT+AGG 0.291103 4_4:+63853537 Msa0694530:three_prime_UTR
TTCAGCCGCAGCAGACTTCT+AGG 0.300158 4_4:+63851660 Msa0694530:CDS
GAGGAAATTAGCGATGGTTT+TGG 0.303370 4_4:-63850619 None:intergenic
GATCAGTCTGTTGTAGTTCT+TGG 0.310407 4_4:+63852770 Msa0694530:CDS
ACAGAAGAATACGGTGAAAA+AGG 0.316862 4_4:-63851440 None:intergenic
TGAATTTAGGCCGTTAGATT+TGG 0.321583 4_4:-63850524 None:intergenic
ACAAATCTCCGGTGCAATTC+CGG 0.337882 4_4:-63851704 None:intergenic
ATTAGTCCAATGTAATGTTT+TGG 0.346875 4_4:+63853278 Msa0694530:three_prime_UTR
GGAATAAACTCTTCCAAAAC+TGG 0.355348 4_4:-63851009 None:intergenic
TAGATGTGAATGTGAAATTA+AGG 0.363270 4_4:-63850308 None:intergenic
ATGGAGTTTGGAGAGTTTAT+CGG 0.366175 4_4:-63850670 None:intergenic
ATGAGTTCTCTAATTTGCTT+TGG 0.366259 4_4:-63852347 None:intergenic
AGAGTTTATCGGAGGTTGTT+TGG 0.367071 4_4:-63850659 None:intergenic
TTCTGCATTGTAGGGTTGTA+GGG 0.369021 4_4:+63853538 Msa0694530:three_prime_UTR
AGAGAGGAAATCATGGAGTT+TGG 0.369125 4_4:-63850682 None:intergenic
TACTACTACAAGATTGAAAA+AGG 0.370440 4_4:-63851391 None:intergenic
GATCGAGAAACAGTTCTAAC+AGG 0.377734 4_4:+63851582 Msa0694530:CDS
GCAGCAAAGATAAGAAGAAT+TGG 0.382485 4_4:+63851103 Msa0694530:CDS
AGAGAACTCATGCAAGTTGA+TGG 0.388920 4_4:+63852359 Msa0694530:CDS
ATGAGAGCCAGACCACAAAA+TGG 0.396148 4_4:-63853461 None:intergenic
GGATGAGTTCTGTTCAGTTA+TGG 0.403657 4_4:+63851124 Msa0694530:CDS
CGCATACTCGGCTGCACGGT+TGG 0.404437 4_4:-63851626 None:intergenic
AAGAGTCTGAGGGAAGGATT+TGG 0.407195 4_4:+63851551 Msa0694530:CDS
TGATGAGATTTATATAAAAT+GGG 0.411321 4_4:-63850423 None:intergenic
AGGAAGAACCTGTAAGGAAT+AGG 0.418786 4_4:+63851504 Msa0694530:CDS
AATACGAACCGTCGTTTAAG+AGG 0.421808 4_4:-63850767 None:intergenic
TTCTTGTTGAGGAGTTAAAA+GGG 0.425040 4_4:-63850466 None:intergenic
GGACTAATAATATCTCTTCT+GGG 0.425648 4_4:-63853263 None:intergenic
CAGTCTGTTGTAGTTCTTGG+AGG 0.429416 4_4:+63852773 Msa0694530:CDS
AGACGCCGTACTGCTACCTT+TGG 0.431495 4_4:-63852831 None:intergenic
AATAATGTAATAAGAAGAAG+TGG 0.434366 4_4:-63850333 None:intergenic
TTCATTTAGCTACAGGATCC+CGG 0.434371 4_4:+63852873 Msa0694530:CDS
TCTTTCCTTTCTGCATTGTA+GGG 0.437001 4_4:+63853530 Msa0694530:three_prime_UTR
AGAGTATCGGTTCTCATAGT+AGG 0.438676 4_4:+63853577 Msa0694530:three_prime_UTR
TACATTCCACGATAAGAATC+AGG 0.439928 4_4:+63853185 Msa0694530:three_prime_UTR
ATCGGAGGTTGTTTGGATTG+TGG 0.443195 4_4:-63850652 None:intergenic
TCTTCACTTCATCATTAGTC+AGG 0.447623 4_4:-63852382 None:intergenic
GAAGAAGAAGATTCTTGTTG+AGG 0.451264 4_4:-63850477 None:intergenic
CATCTCTGAAATTTGTGAAG+TGG 0.455715 4_4:-63853340 None:intergenic
ACATGGAAAAGTTGGTGTAT+AGG 0.458002 4_4:+63852985 Msa0694530:CDS
GAATACATAGAAGAGGCTAT+TGG 0.458107 4_4:+63853087 Msa0694530:three_prime_UTR
GTTAATGCGTTGCAGAAGCT+TGG 0.458197 4_4:+63851725 Msa0694530:CDS
TCCCTCAGACTCTTCACCTC+AGG 0.461715 4_4:-63851542 None:intergenic
CAGTTGGCGATGTCCCTCCC+CGG 0.465539 4_4:-63852892 None:intergenic
AGTCCACTTCATTTAGCTAC+AGG 0.467143 4_4:+63852866 Msa0694530:CDS
TCTTGTAGTAGTAATAGAAA+TGG 0.468798 4_4:+63851401 Msa0694530:CDS
AAAGGTAGCAGTACGGCGTC+TGG 0.473278 4_4:+63852833 Msa0694530:CDS
TGATCAATCTCTTTCTTCAA+AGG 0.473313 4_4:-63851030 None:intergenic
AAATCTAAACTGAGTTCTGC+AGG 0.491660 4_4:-63850579 None:intergenic
TGAGGGAAGGATTTGGTTCG+AGG 0.493121 4_4:+63851558 Msa0694530:CDS
ATTCACAGACATGGAAAAGT+TGG 0.497674 4_4:+63852977 Msa0694530:CDS
GAAGTCTGCTGCGGCTGAAG+TGG 0.500174 4_4:-63851656 None:intergenic
AAGAGAAGGAAGAACCTGTA+AGG 0.506152 4_4:+63851498 Msa0694530:CDS
TCATTTAGCTACAGGATCCC+GGG 0.515125 4_4:+63852874 Msa0694530:CDS
ATCAGCACCTAAAGAAGAGA+AGG 0.517481 4_4:+63851484 Msa0694530:CDS
TGATTCTTATCGTGGAATGT+AGG 0.518021 4_4:-63853183 None:intergenic
TGTAGTAGTAATAGAAATGG+AGG 0.522008 4_4:+63851404 Msa0694530:CDS
CAGAAGCTTGGAGGTTCTCA+AGG 0.522479 4_4:+63851737 Msa0694530:CDS
TTTGCAGATAAAAGTATACA+TGG 0.523808 4_4:+63853030 Msa0694530:three_prime_UTR
CTAGGAAACAGAGGAGATGC+TGG 0.524226 4_4:+63851678 Msa0694530:CDS
CGAGTCGAGAGAGGAAATCA+TGG 0.525149 4_4:-63850689 None:intergenic
CTCCTGAGGTGAAGAGTCTG+AGG 0.527871 4_4:+63851540 Msa0694530:CDS
GTGATAGATAGAGTAGGACT+AGG 0.528949 4_4:-63853638 None:intergenic
GAGTCGTTGTATTGTTCTTG+AGG 0.536314 4_4:-63852806 None:intergenic
TGATCAGTACCACTTGCAGC+TGG 0.538762 4_4:-63852752 None:intergenic
TAATGAATCCTCTACGGTTG+CGG 0.540720 4_4:-63853609 None:intergenic
ATACTCGGCTGCACGGTTGG+AGG 0.545035 4_4:-63851623 None:intergenic
AGTTGGCGATGTCCCTCCCC+GGG 0.547122 4_4:-63852891 None:intergenic
GCTAAATGAAGTGGACTTTG+TGG 0.551266 4_4:-63852860 None:intergenic
AATGCGTTGCAGAAGCTTGG+AGG 0.554542 4_4:+63851728 Msa0694530:CDS
AGAGAAAGCAACGACGAATG+AGG 0.554931 4_4:-63850222 None:intergenic
ACAGAGGAGATGCTGGTCAC+CGG 0.560535 4_4:+63851685 Msa0694530:CDS
GAACTCTCCGCGTATGAAGT+CGG 0.562568 4_4:-63852730 None:intergenic
AAATGACCTGATTCTTATCG+TGG 0.563659 4_4:-63853191 None:intergenic
ATTTATATAAAATGGGAATG+AGG 0.564588 4_4:-63850416 None:intergenic
TCTTGTTGAGGAGTTAAAAG+GGG 0.565631 4_4:-63850465 None:intergenic
GCTGGTCACCGGAATTGCAC+CGG 0.565693 4_4:+63851696 Msa0694530:CDS
CATCCACCAAAACATTACAT+TGG 0.566337 4_4:-63853284 None:intergenic
TAGCTACAGGATCCCGGGGA+GGG 0.570717 4_4:+63852879 Msa0694530:CDS
GCAACGCATTAACAAATCTC+CGG 0.573053 4_4:-63851715 None:intergenic
AGTGGCGCAGCACGCATACT+CGG 0.578170 4_4:-63851638 None:intergenic
GGAGGGACATCGCCAACTGA+AGG 0.584248 4_4:+63852896 Msa0694530:CDS
GTCACAGGAACAGAAGAATA+CGG 0.587549 4_4:-63851449 None:intergenic
GTTTGATTTAGAAAGAGTAT+CGG 0.588174 4_4:+63853564 Msa0694530:three_prime_UTR
TCCATGCTCCTCTTAAACGA+CGG 0.593486 4_4:+63850759 Msa0694530:CDS
AATACATGAATACATAGAAG+AGG 0.594019 4_4:+63853080 Msa0694530:three_prime_UTR
ACAATTTGTGACTGCAGCTG+AGG 0.594577 4_4:+63851367 Msa0694530:CDS
GCAGGAACAACACAACCCAT+TGG 0.594618 4_4:-63850561 None:intergenic
GCCAACTGAAGGTGATAGCA+TGG 0.596672 4_4:+63852907 Msa0694530:CDS
ATGATAGAGGAAATTAGCGA+TGG 0.597212 4_4:-63850625 None:intergenic
TGACAGAAAAGGGAGGAAGA+AGG 0.597320 4_4:+63851344 Msa0694530:intron
AGAAGATAATGAATCCTCTA+CGG 0.597872 4_4:-63853615 None:intergenic
TCTTAATAAATTAGAAACAA+AGG 0.606924 4_4:+63851212 Msa0694530:CDS
GATCCTGTAGCTAAATGAAG+TGG 0.608872 4_4:-63852869 None:intergenic
CATCAACGATCCAAATCTAA+CGG 0.610204 4_4:+63850514 Msa0694530:five_prime_UTR
TCCATGCTATCACCTTCAGT+TGG 0.614177 4_4:-63852908 None:intergenic
GACTAATAATATCTCTTCTG+GGG 0.614864 4_4:-63853262 None:intergenic
TGAATGTGAAATTAAGGATG+CGG 0.615466 4_4:-63850302 None:intergenic
TACAACCCTACAATGCAGAA+AGG 0.616109 4_4:-63853535 None:intergenic
AAAAGTCATATTCACAGACA+TGG 0.616407 4_4:+63852968 Msa0694530:CDS
GATAATGTCACTGTTGTCAC+AGG 0.616977 4_4:-63851464 None:intergenic
AGCACGCATACTCGGCTGCA+CGG 0.618042 4_4:-63851630 None:intergenic
GTTCATCTTCGAGTCGAGAG+AGG 0.619371 4_4:-63850698 None:intergenic
GGATTGTGGAAAGATGATAG+AGG 0.619765 4_4:-63850638 None:intergenic
CAATACAACGACTCTTCCAA+AGG 0.620750 4_4:+63852815 Msa0694530:CDS
TCCTGAGGTGAAGAGTCTGA+GGG 0.627907 4_4:+63851541 Msa0694530:CDS
GAGGGAAGGATTTGGTTCGA+GGG 0.630808 4_4:+63851559 Msa0694530:CDS
TTTATCTGCAAAATTCATGG+TGG 0.631088 4_4:-63853019 None:intergenic
GAGGTGAAGAGTCTGAGGGA+AGG 0.631423 4_4:+63851545 Msa0694530:CDS
TTGCAGATAAAAGTATACAT+GGG 0.633381 4_4:+63853031 Msa0694530:three_prime_UTR
GAGTTTGGAGAGTTTATCGG+AGG 0.638169 4_4:-63850667 None:intergenic
CGGAGAGTTCCAGCTGCAAG+TGG 0.640628 4_4:+63852743 Msa0694530:CDS
TTTAAGAGGAGCATGGAGAG+AGG 0.641505 4_4:-63850753 None:intergenic
CTCTTCCAAAGGTAGCAGTA+CGG 0.648767 4_4:+63852826 Msa0694530:CDS
TCACAAGTGATAGATAGAGT+AGG 0.649562 4_4:-63853644 None:intergenic
ATAATGTAATAAGAAGAAGT+GGG 0.650074 4_4:-63850332 None:intergenic
AGGGAAGGATTTGGTTCGAG+GGG 0.651342 4_4:+63851560 Msa0694530:CDS
CAGATAAAAGTATACATGGG+AGG 0.654402 4_4:+63853034 Msa0694530:three_prime_UTR
ACTTCATACCGCAACCGTAG+AGG 0.656491 4_4:+63853601 Msa0694530:three_prime_UTR
AGCAGACTTCTAGGAAACAG+AGG 0.657415 4_4:+63851669 Msa0694530:CDS
CATTTAGCTACAGGATCCCG+GGG 0.660202 4_4:+63852875 Msa0694530:CDS
ACCGTCGTTTAAGAGGAGCA+TGG 0.663203 4_4:-63850760 None:intergenic
AGAAATACCGACTTCATACG+CGG 0.668200 4_4:+63852723 Msa0694530:intron
ACCACGACAGGAAAACACAA+AGG 0.668802 4_4:-63853397 None:intergenic
ATCGAGAAACAGTTCTAACA+GGG 0.670598 4_4:+63851583 Msa0694530:CDS
TGTTTCCTAGAAGTCTGCTG+CGG 0.688682 4_4:-63851665 None:intergenic
TTAGCTACAGGATCCCGGGG+AGG 0.690072 4_4:+63852878 Msa0694530:CDS
TTGGTGATGACAGAAAAGGG+AGG 0.712344 4_4:+63851337 Msa0694530:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATGAGATTTATATAAAAT+GGG - chr4_4:63850426-63850445 None:intergenic 15.0%
!! TCTTAATAAATTAGAAACAA+AGG + chr4_4:63851212-63851231 Msa0694530:CDS 15.0%
!!! TTTTTTCTTGTCTAATTTTT+AGG - chr4_4:63851281-63851300 None:intergenic 15.0%
!! AATCTATAATATTTCCAAAT+TGG - chr4_4:63851882-63851901 None:intergenic 15.0%
!! ATAATGTAATAAGAAGAAGT+GGG - chr4_4:63850335-63850354 None:intergenic 20.0%
!! AATAATGTAATAAGAAGAAG+TGG - chr4_4:63850336-63850355 None:intergenic 20.0%
!! ATTTATATAAAATGGGAATG+AGG - chr4_4:63850419-63850438 None:intergenic 20.0%
!! GTGATGAGATTTATATAAAA+TGG - chr4_4:63850427-63850446 None:intergenic 20.0%
!!! TCTTTTTGTTTGATGAATTT+AGG - chr4_4:63850540-63850559 None:intergenic 20.0%
!! TTGAATAAAGTTAACAACTT+TGG + chr4_4:63850874-63850893 Msa0694530:intron 20.0%
!! ATTATAGATTAGTGAATTGA+TGG + chr4_4:63851892-63851911 Msa0694530:intron 20.0%
!! TAATAACATTACATCTTGTT+TGG + chr4_4:63852284-63852303 Msa0694530:intron 20.0%
!!! TTTATGTTATTTCCATTTTG+TGG + chr4_4:63853449-63853468 Msa0694530:three_prime_UTR 20.0%
!! TTGTAAGCTGTAAAAAAAAA+AGG + chr4_4:63853483-63853502 Msa0694530:three_prime_UTR 20.0%
!!! TAAAAAAAAAAGGCCTTTTA+AGG + chr4_4:63853493-63853512 Msa0694530:three_prime_UTR 20.0%
!!! AAAAAAAAAAGGCCTTTTAA+GGG + chr4_4:63853494-63853513 Msa0694530:three_prime_UTR 20.0%
! TAGATGTGAATGTGAAATTA+AGG - chr4_4:63850311-63850330 None:intergenic 25.0%
! ATCAAACAAAAAGATCCAAT+GGG + chr4_4:63850546-63850565 Msa0694530:exon 25.0%
!! TGATGATGATGATATTGAAA+GGG - chr4_4:63850793-63850812 None:intergenic 25.0%
!! ATGATGATGATGATATTGAA+AGG - chr4_4:63850794-63850813 None:intergenic 25.0%
!!! ACCCTTTTGAATTTTTTTTG+TGG + chr4_4:63850848-63850867 Msa0694530:intron 25.0%
! CCCACAAAAAAAATTCAAAA+GGG - chr4_4:63850852-63850871 None:intergenic 25.0%
!!! CCCTTTTGAATTTTTTTTGT+GGG + chr4_4:63850849-63850868 Msa0694530:intron 25.0%
! ACCCACAAAAAAAATTCAAA+AGG - chr4_4:63850853-63850872 None:intergenic 25.0%
!!! TTTGGAGTTTTTGATTCAAT+GGG + chr4_4:63850892-63850911 Msa0694530:intron 25.0%
! TACTACTACAAGATTGAAAA+AGG - chr4_4:63851394-63851413 None:intergenic 25.0%
! TCTTGTAGTAGTAATAGAAA+TGG + chr4_4:63851401-63851420 Msa0694530:CDS 25.0%
!!! AAACTTTACTGTTTTTGTGA+TGG + chr4_4:63851770-63851789 Msa0694530:intron 25.0%
!!! ATGCTAGTCTTAAAAGTTTA+AGG + chr4_4:63851930-63851949 Msa0694530:intron 25.0%
! GGATTGTTTAAATTTAGATC+AGG + chr4_4:63852030-63852049 Msa0694530:intron 25.0%
! TGTTTAAATTTAGATCAGGA+AGG + chr4_4:63852034-63852053 Msa0694530:intron 25.0%
! ATATAGACAAGTCAATTTGT+AGG + chr4_4:63852076-63852095 Msa0694530:intron 25.0%
! GTTAAGACCAAAAAACATAT+AGG + chr4_4:63852203-63852222 Msa0694530:intron 25.0%
!! CAATTTTGCATACATAGTTT+TGG + chr4_4:63852254-63852273 Msa0694530:intron 25.0%
! CTTTATTAACCTTAGTTACT+AGG + chr4_4:63852461-63852480 Msa0694530:intron 25.0%
! TTGAGTTATCAAGTTTGAAT+TGG + chr4_4:63852668-63852687 Msa0694530:intron 25.0%
!! ACTTTTATCTGCAAAATTCA+TGG - chr4_4:63853025-63853044 None:intergenic 25.0%
!! TTTGCAGATAAAAGTATACA+TGG + chr4_4:63853030-63853049 Msa0694530:three_prime_UTR 25.0%
!! TTGCAGATAAAAGTATACAT+GGG + chr4_4:63853031-63853050 Msa0694530:three_prime_UTR 25.0%
! AATACATGAATACATAGAAG+AGG + chr4_4:63853080-63853099 Msa0694530:three_prime_UTR 25.0%
!! ATTAGTCCAATGTAATGTTT+TGG + chr4_4:63853278-63853297 Msa0694530:three_prime_UTR 25.0%
!! GTTTGATTTAGAAAGAGTAT+CGG + chr4_4:63853564-63853583 Msa0694530:three_prime_UTR 25.0%
TGAATGTGAAATTAAGGATG+CGG - chr4_4:63850305-63850324 None:intergenic 30.0%
TTCTTGTTGAGGAGTTAAAA+GGG - chr4_4:63850469-63850488 None:intergenic 30.0%
ATTCTTGTTGAGGAGTTAAA+AGG - chr4_4:63850470-63850489 None:intergenic 30.0%
CATCAAACAAAAAGATCCAA+TGG + chr4_4:63850545-63850564 Msa0694530:exon 30.0%
!!! CTTTGGAGTTTTTGATTCAA+TGG + chr4_4:63850891-63850910 Msa0694530:intron 30.0%
!! TTGGAGTTTTTGATTCAATG+GGG + chr4_4:63850893-63850912 Msa0694530:intron 30.0%
! TTTTGTGTATGCAGCAATTT+TGG + chr4_4:63850942-63850961 Msa0694530:intron 30.0%
! TAAAAATTCTGTGCCAGTTT+TGG + chr4_4:63850996-63851015 Msa0694530:CDS 30.0%
TGATCAATCTCTTTCTTCAA+AGG - chr4_4:63851033-63851052 None:intergenic 30.0%
TTCCAACAGTAATCTTAATG+AGG + chr4_4:63851301-63851320 Msa0694530:intron 30.0%
TCCAACAGTAATCTTAATGA+GGG + chr4_4:63851302-63851321 Msa0694530:intron 30.0%
!!! ATGAGGGTTTTGTTTTTGTT+TGG + chr4_4:63851318-63851337 Msa0694530:intron 30.0%
TGTAGTAGTAATAGAAATGG+AGG + chr4_4:63851404-63851423 Msa0694530:CDS 30.0%
!! ACACTCAATTTTGACCAATT+TGG + chr4_4:63851865-63851884 Msa0694530:intron 30.0%
TGATAATCCATAGTTTGGTT+GGG - chr4_4:63852156-63852175 None:intergenic 30.0%
!!! TGATAGGCCTATATGTTTTT+TGG - chr4_4:63852213-63852232 None:intergenic 30.0%
AATTGCAAAGGCAAATCATT+TGG - chr4_4:63852239-63852258 None:intergenic 30.0%
CTATGTATGCAAAATTGCAA+AGG - chr4_4:63852251-63852270 None:intergenic 30.0%
!! ATGAGTTCTCTAATTTGCTT+TGG - chr4_4:63852350-63852369 None:intergenic 30.0%
ACCAAAAACTTACTTGCAAA+TGG - chr4_4:63852409-63852428 None:intergenic 30.0%
TAATAAAGCATGCAGTTAAG+AGG - chr4_4:63852449-63852468 None:intergenic 30.0%
TCATATTGTATGTGCATTCA+TGG + chr4_4:63852610-63852629 Msa0694530:intron 30.0%
AAAAATCTGAGAGTTATAGC+TGG + chr4_4:63852945-63852964 Msa0694530:CDS 30.0%
AAAAGTCATATTCACAGACA+TGG + chr4_4:63852968-63852987 Msa0694530:CDS 30.0%
TTTATCTGCAAAATTCATGG+TGG - chr4_4:63853022-63853041 None:intergenic 30.0%
!!! AGGCTATTGGAATTTTTTGA+AGG + chr4_4:63853100-63853119 Msa0694530:three_prime_UTR 30.0%
GACTAATAATATCTCTTCTG+GGG - chr4_4:63853265-63853284 None:intergenic 30.0%
GGACTAATAATATCTCTTCT+GGG - chr4_4:63853266-63853285 None:intergenic 30.0%
TGGACTAATAATATCTCTTC+TGG - chr4_4:63853267-63853286 None:intergenic 30.0%
AGAAGATAATGAATCCTCTA+CGG - chr4_4:63853618-63853637 None:intergenic 30.0%
TCTTGTTGAGGAGTTAAAAG+GGG - chr4_4:63850468-63850487 None:intergenic 35.0%
GAAGAAGAAGATTCTTGTTG+AGG - chr4_4:63850480-63850499 None:intergenic 35.0%
CATCAACGATCCAAATCTAA+CGG + chr4_4:63850514-63850533 Msa0694530:five_prime_UTR 35.0%
TGAATTTAGGCCGTTAGATT+TGG - chr4_4:63850527-63850546 None:intergenic 35.0%
AAATCTAAACTGAGTTCTGC+AGG - chr4_4:63850582-63850601 None:intergenic 35.0%
! AGGAAATTAGCGATGGTTTT+GGG - chr4_4:63850621-63850640 None:intergenic 35.0%
ATGATAGAGGAAATTAGCGA+TGG - chr4_4:63850628-63850647 None:intergenic 35.0%
ATGGAGTTTGGAGAGTTTAT+CGG - chr4_4:63850673-63850692 None:intergenic 35.0%
!! TTTTGATTCAATGGGGCTAA+TGG + chr4_4:63850900-63850919 Msa0694530:intron 35.0%
TTTGATTCAATGGGGCTAAT+GGG + chr4_4:63850901-63850920 Msa0694530:intron 35.0%
!!! TGCAGCAATTTTGGTTTTGA+AGG + chr4_4:63850951-63850970 Msa0694530:intron 35.0%
!!! AGCAATTTTGGTTTTGAAGG+AGG + chr4_4:63850954-63850973 Msa0694530:intron 35.0%
!!! TTTGGTTTTGAAGGAGGAAT+TGG + chr4_4:63850960-63850979 Msa0694530:CDS 35.0%
GGAATAAACTCTTCCAAAAC+TGG - chr4_4:63851012-63851031 None:intergenic 35.0%
GCAGCAAAGATAAGAAGAAT+TGG + chr4_4:63851103-63851122 Msa0694530:CDS 35.0%
ACCCTCATTAAGATTACTGT+TGG - chr4_4:63851306-63851325 None:intergenic 35.0%
!! TTGTTTGGTGATGACAGAAA+AGG + chr4_4:63851333-63851352 Msa0694530:intron 35.0%
!! TGTTTGGTGATGACAGAAAA+GGG + chr4_4:63851334-63851353 Msa0694530:intron 35.0%
ACAGAAGAATACGGTGAAAA+AGG - chr4_4:63851443-63851462 None:intergenic 35.0%
! ATCGAGAAACAGTTCTAACA+GGG + chr4_4:63851583-63851602 Msa0694530:CDS 35.0%
TCTTATGTAGTTGTGTTGTG+AGG + chr4_4:63851835-63851854 Msa0694530:intron 35.0%
! TTTAGTTCACCAGTGAAGTT+AGG + chr4_4:63852008-63852027 Msa0694530:intron 35.0%
TTAGTTCACCAGTGAAGTTA+GGG + chr4_4:63852009-63852028 Msa0694530:intron 35.0%
TAAACAATCCCTAACTTCAC+TGG - chr4_4:63852020-63852039 None:intergenic 35.0%
TCTATCCCGAATTCAAATCT+TGG + chr4_4:63852099-63852118 Msa0694530:intron 35.0%
AATCTGATCAAGGATTGTTC+CGG - chr4_4:63852125-63852144 None:intergenic 35.0%
GGAGGTTATTAATCTGATCA+AGG - chr4_4:63852135-63852154 None:intergenic 35.0%
CTGATAATCCATAGTTTGGT+TGG - chr4_4:63852157-63852176 None:intergenic 35.0%
!!! GTTTTTTGGTCTTAACCTTC+CGG - chr4_4:63852199-63852218 None:intergenic 35.0%
GCAAATCATTTGGACTTGAT+AGG - chr4_4:63852229-63852248 None:intergenic 35.0%
TCTTCACTTCATCATTAGTC+AGG - chr4_4:63852385-63852404 None:intergenic 35.0%
! GCCATTTGCAAGTAAGTTTT+TGG + chr4_4:63852405-63852424 Msa0694530:intron 35.0%
CATCAAACACCTAGTAACTA+AGG - chr4_4:63852473-63852492 None:intergenic 35.0%
AGTTACTAGGTGTTTGATGA+TGG + chr4_4:63852474-63852493 Msa0694530:intron 35.0%
! AGGCATTTAGGATTGAGATT+TGG + chr4_4:63852645-63852664 Msa0694530:intron 35.0%
TTGTAGTTCTTGGAGGTTTA+TGG + chr4_4:63852780-63852799 Msa0694530:CDS 35.0%
ATTCACAGACATGGAAAAGT+TGG + chr4_4:63852977-63852996 Msa0694530:CDS 35.0%
!! ACATGGAAAAGTTGGTGTAT+AGG + chr4_4:63852985-63853004 Msa0694530:CDS 35.0%
! CAGATAAAAGTATACATGGG+AGG + chr4_4:63853034-63853053 Msa0694530:three_prime_UTR 35.0%
GAATACATAGAAGAGGCTAT+TGG + chr4_4:63853087-63853106 Msa0694530:three_prime_UTR 35.0%
TGATTCTTATCGTGGAATGT+AGG - chr4_4:63853186-63853205 None:intergenic 35.0%
TACATTCCACGATAAGAATC+AGG + chr4_4:63853185-63853204 Msa0694530:three_prime_UTR 35.0%
AAATGACCTGATTCTTATCG+TGG - chr4_4:63853194-63853213 None:intergenic 35.0%
!! AGTCCAATGTAATGTTTTGG+TGG + chr4_4:63853281-63853300 Msa0694530:three_prime_UTR 35.0%
CATCCACCAAAACATTACAT+TGG - chr4_4:63853287-63853306 None:intergenic 35.0%
!! CATCTCTGAAATTTGTGAAG+TGG - chr4_4:63853343-63853362 None:intergenic 35.0%
! TTTTCTCAAAGACACTGAAC+AGG + chr4_4:63853421-63853440 Msa0694530:three_prime_UTR 35.0%
!! GTTATTTCCATTTTGTGGTC+TGG + chr4_4:63853454-63853473 Msa0694530:three_prime_UTR 35.0%
TGACAGAATTTGCCCTTAAA+AGG - chr4_4:63853509-63853528 None:intergenic 35.0%
TCTTTCCTTTCTGCATTGTA+GGG + chr4_4:63853530-63853549 Msa0694530:three_prime_UTR 35.0%
TCACAAGTGATAGATAGAGT+AGG - chr4_4:63853647-63853666 None:intergenic 35.0%
!!! ATGGTTTTGGGAACGAAAGT+TGG - chr4_4:63850609-63850628 None:intergenic 40.0%
! GAGGAAATTAGCGATGGTTT+TGG - chr4_4:63850622-63850641 None:intergenic 40.0%
GGATTGTGGAAAGATGATAG+AGG - chr4_4:63850641-63850660 None:intergenic 40.0%
AGAGTTTATCGGAGGTTGTT+TGG - chr4_4:63850662-63850681 None:intergenic 40.0%
AGAGAGGAAATCATGGAGTT+TGG - chr4_4:63850685-63850704 None:intergenic 40.0%
AATACGAACCGTCGTTTAAG+AGG - chr4_4:63850770-63850789 None:intergenic 40.0%
GGATGAGTTCTGTTCAGTTA+TGG + chr4_4:63851124-63851143 Msa0694530:CDS 40.0%
GTCACAGGAACAGAAGAATA+CGG - chr4_4:63851452-63851471 None:intergenic 40.0%
GATAATGTCACTGTTGTCAC+AGG - chr4_4:63851467-63851486 None:intergenic 40.0%
ATCAGCACCTAAAGAAGAGA+AGG + chr4_4:63851484-63851503 Msa0694530:CDS 40.0%
GGTTCTTCCTTCTCTTCTTT+AGG - chr4_4:63851494-63851513 None:intergenic 40.0%
AAGAGAAGGAAGAACCTGTA+AGG + chr4_4:63851498-63851517 Msa0694530:CDS 40.0%
AGGAAGAACCTGTAAGGAAT+AGG + chr4_4:63851504-63851523 Msa0694530:CDS 40.0%
AAAGAGAGCCTATTCCTTAC+AGG - chr4_4:63851515-63851534 None:intergenic 40.0%
! GATCGAGAAACAGTTCTAAC+AGG + chr4_4:63851582-63851601 Msa0694530:CDS 40.0%
GCAACGCATTAACAAATCTC+CGG - chr4_4:63851718-63851737 None:intergenic 40.0%
! TAGTGAATTGATGGATGTGC+TGG + chr4_4:63851901-63851920 Msa0694530:intron 40.0%
AGATCAGGAAGGAGACAAAT+AGG + chr4_4:63852045-63852064 Msa0694530:intron 40.0%
TTCCGGCCAAGATTTGAATT+CGG - chr4_4:63852108-63852127 None:intergenic 40.0%
ATAACCTCCCAACCAAACTA+TGG + chr4_4:63852146-63852165 Msa0694530:intron 40.0%
TAATCCATAGTTTGGTTGGG+AGG - chr4_4:63852153-63852172 None:intergenic 40.0%
GGGACTGATAATCCATAGTT+TGG - chr4_4:63852161-63852180 None:intergenic 40.0%
! AGAGAACTCATGCAAGTTGA+TGG + chr4_4:63852359-63852378 Msa0694530:CDS 40.0%
ATTCATGGTGTCTGGTTATG+AGG + chr4_4:63852625-63852644 Msa0694530:intron 40.0%
! TGTCTGGTTATGAGGCATTT+AGG + chr4_4:63852633-63852652 Msa0694530:intron 40.0%
!! AGAAATACCGACTTCATACG+CGG + chr4_4:63852723-63852742 Msa0694530:intron 40.0%
GATCAGTCTGTTGTAGTTCT+TGG + chr4_4:63852770-63852789 Msa0694530:CDS 40.0%
GAGTCGTTGTATTGTTCTTG+AGG - chr4_4:63852809-63852828 None:intergenic 40.0%
CAATACAACGACTCTTCCAA+AGG + chr4_4:63852815-63852834 Msa0694530:CDS 40.0%
! GCTAAATGAAGTGGACTTTG+TGG - chr4_4:63852863-63852882 None:intergenic 40.0%
AGTCCACTTCATTTAGCTAC+AGG + chr4_4:63852866-63852885 Msa0694530:CDS 40.0%
GATCCTGTAGCTAAATGAAG+TGG - chr4_4:63852872-63852891 None:intergenic 40.0%
TTCATTTAGCTACAGGATCC+CGG + chr4_4:63852873-63852892 Msa0694530:CDS 40.0%
! CTTTGAGAAAAAACCACGAC+AGG - chr4_4:63853412-63853431 None:intergenic 40.0%
CTCTTTCCTTTCTGCATTGT+AGG + chr4_4:63853529-63853548 Msa0694530:three_prime_UTR 40.0%
TACAACCCTACAATGCAGAA+AGG - chr4_4:63853538-63853557 None:intergenic 40.0%
TTTCTGCATTGTAGGGTTGT+AGG + chr4_4:63853537-63853556 Msa0694530:three_prime_UTR 40.0%
TTCTGCATTGTAGGGTTGTA+GGG + chr4_4:63853538-63853557 Msa0694530:three_prime_UTR 40.0%
!! AGAGTATCGGTTCTCATAGT+AGG + chr4_4:63853577-63853596 Msa0694530:three_prime_UTR 40.0%
TAATGAATCCTCTACGGTTG+CGG - chr4_4:63853612-63853631 None:intergenic 40.0%
GTGATAGATAGAGTAGGACT+AGG - chr4_4:63853641-63853660 None:intergenic 40.0%
AGAGAAAGCAACGACGAATG+AGG - chr4_4:63850225-63850244 None:intergenic 45.0%
ATCGGAGGTTGTTTGGATTG+TGG - chr4_4:63850655-63850674 None:intergenic 45.0%
GAGTTTGGAGAGTTTATCGG+AGG - chr4_4:63850670-63850689 None:intergenic 45.0%
! TTTAAGAGGAGCATGGAGAG+AGG - chr4_4:63850756-63850775 None:intergenic 45.0%
TCCATGCTCCTCTTAAACGA+CGG + chr4_4:63850759-63850778 Msa0694530:CDS 45.0%
!! TTGGTGATGACAGAAAAGGG+AGG + chr4_4:63851337-63851356 Msa0694530:intron 45.0%
TGACAGAAAAGGGAGGAAGA+AGG + chr4_4:63851344-63851363 Msa0694530:intron 45.0%
ACAATTTGTGACTGCAGCTG+AGG + chr4_4:63851367-63851386 Msa0694530:CDS 45.0%
AAGAGTCTGAGGGAAGGATT+TGG + chr4_4:63851551-63851570 Msa0694530:CDS 45.0%
TGTTTCCTAGAAGTCTGCTG+CGG - chr4_4:63851668-63851687 None:intergenic 45.0%
! AGCAGACTTCTAGGAAACAG+AGG + chr4_4:63851669-63851688 Msa0694530:CDS 45.0%
ACAAATCTCCGGTGCAATTC+CGG - chr4_4:63851707-63851726 None:intergenic 45.0%
GTTAATGCGTTGCAGAAGCT+TGG + chr4_4:63851725-63851744 Msa0694530:CDS 45.0%
TCCCGAATTCAAATCTTGGC+CGG + chr4_4:63852103-63852122 Msa0694530:intron 45.0%
TCCGGCCAAGATTTGAATTC+GGG - chr4_4:63852107-63852126 None:intergenic 45.0%
TCTTAACCTTCCGGTTCCTA+GGG - chr4_4:63852190-63852209 None:intergenic 45.0%
GTATGTGCATTCATGGTGTC+TGG + chr4_4:63852617-63852636 Msa0694530:intron 45.0%
CAGTCTGTTGTAGTTCTTGG+AGG + chr4_4:63852773-63852792 Msa0694530:CDS 45.0%
CTCTTCCAAAGGTAGCAGTA+CGG + chr4_4:63852826-63852845 Msa0694530:CDS 45.0%
TCATTTAGCTACAGGATCCC+GGG + chr4_4:63852874-63852893 Msa0694530:CDS 45.0%
TCCATGCTATCACCTTCAGT+TGG - chr4_4:63852911-63852930 None:intergenic 45.0%
! ACCTTTGTGTTTTCCTGTCG+TGG + chr4_4:63853396-63853415 Msa0694530:three_prime_UTR 45.0%
ACCACGACAGGAAAACACAA+AGG - chr4_4:63853400-63853419 None:intergenic 45.0%
ATGAGAGCCAGACCACAAAA+TGG - chr4_4:63853464-63853483 None:intergenic 45.0%
GCAGGAACAACACAACCCAT+TGG - chr4_4:63850564-63850583 None:intergenic 50.0%
CGAGTCGAGAGAGGAAATCA+TGG - chr4_4:63850692-63850711 None:intergenic 50.0%
GTTCATCTTCGAGTCGAGAG+AGG - chr4_4:63850701-63850720 None:intergenic 50.0%
ACCGTCGTTTAAGAGGAGCA+TGG - chr4_4:63850763-63850782 None:intergenic 50.0%
! GCTCTCTTTTCTGACTCCTG+AGG + chr4_4:63851526-63851545 Msa0694530:CDS 50.0%
TCCTGAGGTGAAGAGTCTGA+GGG + chr4_4:63851541-63851560 Msa0694530:CDS 50.0%
! TGAGGGAAGGATTTGGTTCG+AGG + chr4_4:63851558-63851577 Msa0694530:CDS 50.0%
! GAGGGAAGGATTTGGTTCGA+GGG + chr4_4:63851559-63851578 Msa0694530:CDS 50.0%
! AGGGAAGGATTTGGTTCGAG+GGG + chr4_4:63851560-63851579 Msa0694530:CDS 50.0%
CTAGGAAACAGAGGAGATGC+TGG + chr4_4:63851678-63851697 Msa0694530:CDS 50.0%
AATGCGTTGCAGAAGCTTGG+AGG + chr4_4:63851728-63851747 Msa0694530:CDS 50.0%
CAGAAGCTTGGAGGTTCTCA+AGG + chr4_4:63851737-63851756 Msa0694530:CDS 50.0%
TATCAGTCCCTCCTTTCCCT+AGG + chr4_4:63852171-63852190 Msa0694530:intron 50.0%
GTCTTAACCTTCCGGTTCCT+AGG - chr4_4:63852191-63852210 None:intergenic 50.0%
GAACTCTCCGCGTATGAAGT+CGG - chr4_4:63852733-63852752 None:intergenic 50.0%
TGATCAGTACCACTTGCAGC+TGG - chr4_4:63852755-63852774 None:intergenic 50.0%
CATTTAGCTACAGGATCCCG+GGG + chr4_4:63852875-63852894 Msa0694530:CDS 50.0%
GCCAACTGAAGGTGATAGCA+TGG + chr4_4:63852907-63852926 Msa0694530:CDS 50.0%
ACTTCATACCGCAACCGTAG+AGG + chr4_4:63853601-63853620 Msa0694530:three_prime_UTR 50.0%
CTCCTGAGGTGAAGAGTCTG+AGG + chr4_4:63851540-63851559 Msa0694530:CDS 55.0%
TCCCTCAGACTCTTCACCTC+AGG - chr4_4:63851545-63851564 None:intergenic 55.0%
GAGGTGAAGAGTCTGAGGGA+AGG + chr4_4:63851545-63851564 Msa0694530:CDS 55.0%
TTCAGCCGCAGCAGACTTCT+AGG + chr4_4:63851660-63851679 Msa0694530:CDS 55.0%
ACAGAGGAGATGCTGGTCAC+CGG + chr4_4:63851685-63851704 Msa0694530:CDS 55.0%
TCCCTCCTTTCCCTAGGAAC+CGG + chr4_4:63852177-63852196 Msa0694530:intron 55.0%
TCCGGTTCCTAGGGAAAGGA+GGG - chr4_4:63852181-63852200 None:intergenic 55.0%
TTCCGGTTCCTAGGGAAAGG+AGG - chr4_4:63852182-63852201 None:intergenic 55.0%
TCCTTTCCCTAGGAACCGGA+AGG + chr4_4:63852181-63852200 Msa0694530:intron 55.0%
ACCTTCCGGTTCCTAGGGAA+AGG - chr4_4:63852185-63852204 None:intergenic 55.0%
AGACGCCGTACTGCTACCTT+TGG - chr4_4:63852834-63852853 None:intergenic 55.0%
AAAGGTAGCAGTACGGCGTC+TGG + chr4_4:63852833-63852852 Msa0694530:CDS 55.0%
ATACTCGGCTGCACGGTTGG+AGG - chr4_4:63851626-63851645 None:intergenic 60.0%
AGCACGCATACTCGGCTGCA+CGG - chr4_4:63851633-63851652 None:intergenic 60.0%
AGTGGCGCAGCACGCATACT+CGG - chr4_4:63851641-63851660 None:intergenic 60.0%
GAAGTCTGCTGCGGCTGAAG+TGG - chr4_4:63851659-63851678 None:intergenic 60.0%
GCTGGTCACCGGAATTGCAC+CGG + chr4_4:63851696-63851715 Msa0694530:CDS 60.0%
CGGAGAGTTCCAGCTGCAAG+TGG + chr4_4:63852743-63852762 Msa0694530:CDS 60.0%
TTAGCTACAGGATCCCGGGG+AGG + chr4_4:63852878-63852897 Msa0694530:CDS 60.0%
TAGCTACAGGATCCCGGGGA+GGG + chr4_4:63852879-63852898 Msa0694530:CDS 60.0%
GGAGGGACATCGCCAACTGA+AGG + chr4_4:63852896-63852915 Msa0694530:CDS 60.0%
CGCATACTCGGCTGCACGGT+TGG - chr4_4:63851629-63851648 None:intergenic 65.0%
AGTTGGCGATGTCCCTCCCC+GGG - chr4_4:63852894-63852913 None:intergenic 65.0%
CAGTTGGCGATGTCCCTCCC+CGG - chr4_4:63852895-63852914 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 63850215 63853673 63850215 ID=Msa0694530;Name=Msa0694530
chr4_4 mRNA 63850215 63853673 63850215 ID=Msa0694530-mRNA-1;Parent=Msa0694530;Name=Msa0694530-mRNA-1;_AED=0.12;_eAED=0.12;_QI=349|0.75|0.8|1|1|1|5|667|420
chr4_4 exon 63850215 63850780 63850215 ID=Msa0694530-mRNA-1:exon:18123;Parent=Msa0694530-mRNA-1
chr4_4 exon 63850956 63851233 63850956 ID=Msa0694530-mRNA-1:exon:18124;Parent=Msa0694530-mRNA-1
chr4_4 exon 63851350 63851758 63851350 ID=Msa0694530-mRNA-1:exon:18125;Parent=Msa0694530-mRNA-1
chr4_4 exon 63852339 63852415 63852339 ID=Msa0694530-mRNA-1:exon:18126;Parent=Msa0694530-mRNA-1
chr4_4 exon 63852725 63853673 63852725 ID=Msa0694530-mRNA-1:exon:18127;Parent=Msa0694530-mRNA-1
chr4_4 five_prime_UTR 63850215 63850563 63850215 ID=Msa0694530-mRNA-1:five_prime_utr;Parent=Msa0694530-mRNA-1
chr4_4 CDS 63850564 63850780 63850564 ID=Msa0694530-mRNA-1:cds;Parent=Msa0694530-mRNA-1
chr4_4 CDS 63850956 63851233 63850956 ID=Msa0694530-mRNA-1:cds;Parent=Msa0694530-mRNA-1
chr4_4 CDS 63851350 63851758 63851350 ID=Msa0694530-mRNA-1:cds;Parent=Msa0694530-mRNA-1
chr4_4 CDS 63852339 63852415 63852339 ID=Msa0694530-mRNA-1:cds;Parent=Msa0694530-mRNA-1
chr4_4 CDS 63852725 63853006 63852725 ID=Msa0694530-mRNA-1:cds;Parent=Msa0694530-mRNA-1
chr4_4 three_prime_UTR 63853007 63853673 63853007 ID=Msa0694530-mRNA-1:three_prime_utr;Parent=Msa0694530-mRNA-1
Gene Sequence

>Msa0694530

ATGGGTTGTGTTGTTCCTGCAGAACTCAGTTTAGATTTAAGACCAACTTTCGTTCCCAAAACCATCGCTAATTTCCTCTATCATCTTTCCACAATCCAAACAACCTCCGATAAACTCTCCAAACTCCATGATTTCCTCTCTCGACTCGAAGATGAACTCAATAAGATCGATGCTTTCAAACGTGAACTACCTCTCTCCATGCTCCTCTTAAACGACGCAATTTTGGTTTTGAAGGAGGAATTGGAAAAATGTAGAAGTAAAAATTCTGTGCCAGTTTTGGAAGAGTTTATTCCTTTGAAGAAAGAGATTGATCAGAGTGAAGAGAATAATAATAATGATAGAGACAAGAACAACAACAATGAATGCAGCAAAGATAAGAAGAATTGGATGAGTTCTGTTCAGTTATGGAATAACAACACAACAACTTCTAATAATAATGTCTCTGATCATCATCATAAACATAACAATCATAATCTTAATAAATTAGAAACAAAGAAAAGGGAGGAAGAAGGACAATTTGTGACTGCAGCTGAGGACCTTTTTCAATCTTGTAGTAGTAATAGAAATGGAGGAAGTGCTTTTATGCCTTTTTCACCGTATTCTTCTGTTCCTGTGACAACAGTGACATTATCAGCACCTAAAGAAGAGAAGGAAGAACCTGTAAGGAATAGGCTCTCTTTTCTGACTCCTGAGGTGAAGAGTCTGAGGGAAGGATTTGGTTCGAGGGGATCGAGAAACAGTTCTAACAGGGCAGTTTCGTCGTCGTCTCCTCCAACCGTGCAGCCGAGTATGCGTGCTGCGCCACTTCAGCCGCAGCAGACTTCTAGGAAACAGAGGAGATGCTGGTCACCGGAATTGCACCGGAGATTTGTTAATGCGTTGCAGAAGCTTGGAGGTTCTCAAGCAGCAACTCCAAAGCAAATTAGAGAACTCATGCAAGTTGATGGCCTGACTAATGATGAAGTGAAGAGCCATTTGCAAAAATACCGACTTCATACGCGGAGAGTTCCAGCTGCAAGTGGTACTGATCAGTCTGTTGTAGTTCTTGGAGGTTTATGGATGCCTCAAGAACAATACAACGACTCTTCCAAAGGTAGCAGTACGGCGTCTGGTTCTCCACAAAGTCCACTTCATTTAGCTACAGGATCCCGGGGAGGGACATCGCCAACTGAAGGTGATAGCATGGACGATGACGATGATGAAAAATCTGAGAGTTATAGCTGGAAAAGTCATATTCACAGACATGGAAAAGTTGGTGTATAG
Protein sequence

>Msa0694530

MGCVVPAELSLDLRPTFVPKTIANFLYHLSTIQTTSDKLSKLHDFLSRLEDELNKIDAFKRELPLSMLLLNDAILVLKEELEKCRSKNSVPVLEEFIPLKKEIDQSEENNNNDRDKNNNNECSKDKKNWMSSVQLWNNNTTTSNNNVSDHHHKHNNHNLNKLETKKREEEGQFVTAAEDLFQSCSSNRNGGSAFMPFSPYSSVPVTTVTLSAPKEEKEEPVRNRLSFLTPEVKSLREGFGSRGSRNSSNRAVSSSSPPTVQPSMRAAPLQPQQTSRKQRRCWSPELHRRFVNALQKLGGSQAATPKQIRELMQVDGLTNDEVKSHLQKYRLHTRRVPAASGTDQSVVVLGGLWMPQEQYNDSSKGSSTASGSPQSPLHLATGSRGGTSPTEGDSMDDDDDEKSESYSWKSHIHRHGKVGV*