AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0557400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0557400 MtrunA17_Chr8g0379681 81.148 366 21 7 1 366 1 318 4.31e-176 494
Msa0557400 MtrunA17_Chr2g0284471 48.523 237 92 7 149 366 82 307 2.10e-53 181
Msa0557400 MtrunA17_Chr8g0379691 96.154 52 2 0 365 416 14 65 1.34e-27 104
Msa0557400 MtrunA17_Chr5g0432531 42.222 135 57 5 233 365 130 245 1.16e-22 96.3
Msa0557400 MtrunA17_Chr7g0244571 63.333 60 22 0 306 365 281 340 4.75e-20 90.9
Msa0557400 MtrunA17_Chr4g0013081 62.712 59 22 0 307 365 315 373 8.47e-19 87.4
Msa0557400 MtrunA17_Chr8g0390531 56.667 60 26 0 306 365 258 317 3.06e-15 76.3
Msa0557400 MtrunA17_Chr1g0177881 50.769 65 32 0 304 368 100 164 2.45e-13 68.2
Msa0557400 MtrunA17_Chr4g0025901 43.939 66 37 0 305 370 98 163 1.56e-12 65.9
Msa0557400 MtrunA17_Chr5g0435131 39.241 79 48 0 297 375 82 160 3.42e-12 64.3
Msa0557400 MtrunA17_Chr7g0267111 38.961 77 47 0 292 368 96 172 4.52e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0557400 AT4G18830.1 52.143 140 61 3 232 369 210 345 2.55e-37 139
Msa0557400 AT5G58360.1 47.573 103 50 1 268 366 182 284 2.28e-21 94.0
Msa0557400 AT1G06920.1 56.716 67 29 0 300 366 237 303 2.39e-20 91.7
Msa0557400 AT2G30400.1 56.716 67 29 0 299 365 245 311 7.66e-19 87.4
Msa0557400 AT5G01840.1 60.714 56 22 0 310 365 202 257 7.93e-16 77.8
Msa0557400 AT5G19650.1 55.172 58 26 0 306 363 154 211 8.03e-16 76.6
Msa0557400 AT3G52525.1 50.794 63 31 0 301 363 61 123 5.61e-12 63.9
Msa0557400 AT2G18500.1 49.153 59 30 0 305 363 225 283 8.71e-12 66.2

Find 66 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAGTTGTGAAGTGTTCTT+TGG 0.266311 4_1:+55768383 Msa0557400:CDS
GAGGGAAATAATTGGGTTCT+TGG 0.266493 4_1:-55768060 None:intergenic
AACAACTCTTCCATTTCTTC+TGG 0.277466 4_1:-55768468 None:intergenic
CTACATAAGAGGGAAATAAT+TGG 0.293355 4_1:-55768068 None:intergenic
TTTCAAAGTGTCAGAGAAAA+TGG 0.293837 4_1:+55768114 Msa0557400:CDS
ACATCCAAACTGTACATTAC+TGG 0.306963 4_1:-55767814 None:intergenic
GGGTTCTTGGTGATTCAAAT+TGG 0.318117 4_1:-55768047 None:intergenic
TCGACTTTAGTAGCCATCTT+TGG 0.329186 4_1:-55768255 None:intergenic
GATAAGCCAGCCAGAAGAAA+TGG 0.364577 4_1:+55768458 Msa0557400:CDS
TATGTAGATGCAGAAAGTTC+TGG 0.372162 4_1:+55768084 Msa0557400:CDS
CTTACCTTGGAACTCTGCTT+TGG 0.374018 4_1:-55768222 None:intergenic
AATGATATCATGGTATTCAT+TGG 0.398859 4_1:-55768514 None:intergenic
GTGGTGAGGATGATGCATTC+TGG 0.406825 4_1:+55767728 Msa0557400:CDS
GAAGCCAGTAATGTACAGTT+TGG 0.411136 4_1:+55767810 Msa0557400:CDS
ATTCAGCAATATAGTAGTAT+TGG 0.419698 4_1:-55770468 None:intergenic
GATGCAGAAAGTTCTGGTCT+TGG 0.420949 4_1:+55768090 Msa0557400:CDS
CAATTTCCTTGTGGGAATAG+AGG 0.421459 4_1:+55767691 Msa0557400:CDS
ACTTTCTGCATCTACATAAG+AGG 0.429055 4_1:-55768079 None:intergenic
GTTCATCACAATTTCCTTGT+GGG 0.429553 4_1:+55767683 Msa0557400:CDS
TGGAGAATGTACTCTTACCT+TGG 0.430524 4_1:-55768235 None:intergenic
ACTCTTCCATTTCTTCTGGC+TGG 0.432481 4_1:-55768464 None:intergenic
AGAGGATACATGAGAGATGA+AGG 0.442815 4_1:-55767521 None:intergenic
TACATAAGAGGGAAATAATT+GGG 0.446625 4_1:-55768067 None:intergenic
GAAAGAAGAAGAGGAAATTG+TGG 0.448238 4_1:+55768332 Msa0557400:CDS
GCATTCTGGAGATTGTCATT+TGG 0.470215 4_1:+55767742 Msa0557400:CDS
TTCCTTGTGGGAATAGAGGC+AGG 0.471947 4_1:+55767695 Msa0557400:CDS
ATTGTTGTCGTTGCATCAGA+TGG 0.473177 4_1:-55767625 None:intergenic
AAAGATGAAGAAAGAAGAAG+AGG 0.484787 4_1:+55768323 Msa0557400:CDS
TTAAAGCAAAGGTTAACAAA+AGG 0.494673 4_1:+55768157 Msa0557400:CDS
TAAATGTAAATCCTTAGAAA+AGG 0.497808 4_1:+55768017 Msa0557400:CDS
ATGCTTTGATAATGATATCA+TGG 0.506592 4_1:-55768524 None:intergenic
AAGAGTACATTCTCCAAAGA+TGG 0.518216 4_1:+55768242 Msa0557400:CDS
GAAGAAGCTGAAGCTGAAGA+AGG 0.519649 4_1:-55767481 None:intergenic
ATAGAGATTCAGTTCATCCT+AGG 0.520159 4_1:+55770489 Msa0557400:CDS
AAGCAAAGGTTAACAAAAGG+AGG 0.522101 4_1:+55768160 Msa0557400:CDS
CAAGAAATCAAACAATGAAG+TGG 0.530510 4_1:+55767446 None:intergenic
ACTCAGAACTCCCCGTCTGA+TGG 0.532872 4_1:+55767661 Msa0557400:CDS
AGTCGAAATACGCAAAATGA+AGG 0.533126 4_1:+55768272 Msa0557400:CDS
TTGTGGAGGAAACAGAAGAA+GGG 0.535857 4_1:+55768349 Msa0557400:CDS
CATTATCAAAGCATTTCGAC+AGG 0.549940 4_1:+55768533 Msa0557400:CDS
TTGTTGTCGTTGCATCAGAT+GGG 0.555449 4_1:-55767624 None:intergenic
ATGTGAGGAGTTTAAAGCAA+AGG 0.562245 4_1:+55768146 Msa0557400:CDS
CTTTCTGCATCTACATAAGA+GGG 0.562393 4_1:-55768078 None:intergenic
GAATCTCTAAAATCTTGTTG+TGG 0.573719 4_1:-55768408 None:intergenic
ATTGTGGAGGAAACAGAAGA+AGG 0.577830 4_1:+55768348 Msa0557400:CDS
AGGAAGTTGAGTGCTTAAAG+AGG 0.596150 4_1:+55767905 Msa0557400:CDS
GCTTAAAGAGGAGATATGAG+AGG 0.596446 4_1:+55767917 Msa0557400:CDS
GGTTCATCACAATTTCCTTG+TGG 0.598854 4_1:+55767682 Msa0557400:CDS
AGAAGAAGAGGAAATTGTGG+AGG 0.599408 4_1:+55768335 Msa0557400:CDS
AATCAAACAATGAAGTGGAG+TGG 0.599426 4_1:+55767451 None:intergenic
AGCAAAGGTTAACAAAAGGA+GGG 0.601137 4_1:+55768161 Msa0557400:CDS
GATACATGAGAGATGAAGGA+AGG 0.612433 4_1:-55767517 None:intergenic
AATTGTGATGAACCATCAGA+CGG 0.617022 4_1:-55767673 None:intergenic
ATTGTGATGAACCATCAGAC+GGG 0.626786 4_1:-55767672 None:intergenic
GAAACCAAAGCAGAGTTCCA+AGG 0.627313 4_1:+55768218 Msa0557400:CDS
AGTTTGGATGTGAAAAGACA+TGG 0.628614 4_1:+55767826 Msa0557400:CDS
GAAATTACAGAATGCAGCAG+AGG 0.634808 4_1:+55767972 Msa0557400:CDS
TAATGAAACAAAGAGTGCAA+AGG 0.636359 4_1:+55767885 Msa0557400:CDS
ATCAGACGGGGAGTTCTGAG+TGG 0.651626 4_1:-55767659 None:intergenic
TGAGCAGAGAATTACAGAGG+AGG 0.661098 4_1:+55768196 Msa0557400:CDS
TCAGACGGGGAGTTCTGAGT+GGG 0.663234 4_1:-55767658 None:intergenic
AACCTGCCTCTATTCCCACA+AGG 0.669707 4_1:-55767697 None:intergenic
TGTTGTCGTTGCATCAGATG+GGG 0.671686 4_1:-55767623 None:intergenic
ATGTGAGCAGAGAATTACAG+AGG 0.684434 4_1:+55768193 Msa0557400:CDS
AAATGGTGTGAATCAATGTG+AGG 0.711307 4_1:+55768131 Msa0557400:CDS
TTGTGATGAACCATCAGACG+GGG 0.714122 4_1:-55767671 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAACTATGTA+TGG + chr4_1:55769059-55769078 Msa0557400:intron 10.0%
!! TATTTAGTTATCAAATTTAT+TGG - chr4_1:55769134-55769153 None:intergenic 10.0%
!! AAAAAGAAATTCAAAATTTA+AGG + chr4_1:55769855-55769874 Msa0557400:intron 10.0%
!! ATAGTATATTTATTAATTGC+AGG - chr4_1:55769024-55769043 None:intergenic 15.0%
!!! TAATATTGTTTTATGTAATC+AGG - chr4_1:55769197-55769216 None:intergenic 15.0%
!! ATATATCAATATATCATATC+AGG + chr4_1:55769557-55769576 Msa0557400:intron 15.0%
!! TGTTTGATATTATAATAGTT+TGG - chr4_1:55769616-55769635 None:intergenic 15.0%
!! ATAAAAACAATAAAAGCAAA+AGG + chr4_1:55769763-55769782 Msa0557400:intron 15.0%
!! TTATGTCTTTATAGTTAAAT+AGG - chr4_1:55770103-55770122 None:intergenic 15.0%
!! ATAAAGACATAAAACAAAAA+TGG + chr4_1:55770111-55770130 Msa0557400:intron 15.0%
!!! TATCTTTACTTTTCTTCTTT+TGG - chr4_1:55767786-55767805 None:intergenic 20.0%
!! TAAATGTAAATCCTTAGAAA+AGG + chr4_1:55768017-55768036 Msa0557400:CDS 20.0%
!! ATTTATTACACAAACTTGAA+TGG - chr4_1:55768575-55768594 None:intergenic 20.0%
!! ATAAATTACAGATTAGAACT+TGG - chr4_1:55768664-55768683 None:intergenic 20.0%
!! ATGTATTTACTCTTACTATT+AGG - chr4_1:55768993-55769012 None:intergenic 20.0%
!! TAATGACATAATCTACAATA+TGG + chr4_1:55769083-55769102 Msa0557400:intron 20.0%
!! AATGACATAATCTACAATAT+GGG + chr4_1:55769084-55769103 Msa0557400:intron 20.0%
!! GCTTATATACAAAAAAAAAG+AGG - chr4_1:55769261-55769280 None:intergenic 20.0%
!! ATAAACAGTAATACATCAAT+GGG - chr4_1:55770273-55770292 None:intergenic 20.0%
!! TATAAACAGTAATACATCAA+TGG - chr4_1:55770274-55770293 None:intergenic 20.0%
! TACATAAGAGGGAAATAATT+GGG - chr4_1:55768070-55768089 None:intergenic 25.0%
! TTAAAGCAAAGGTTAACAAA+AGG + chr4_1:55768157-55768176 Msa0557400:CDS 25.0%
! AATGATATCATGGTATTCAT+TGG - chr4_1:55768517-55768536 None:intergenic 25.0%
!!! ATGCTTTGATAATGATATCA+TGG - chr4_1:55768527-55768546 None:intergenic 25.0%
! CAACTATTTACAATTCTAAC+AGG - chr4_1:55768633-55768652 None:intergenic 25.0%
!!! CTTGATACTATAGTTCTAAA+CGG - chr4_1:55768749-55768768 None:intergenic 25.0%
! TATAATTATTCTTCAGCAGT+AGG + chr4_1:55768818-55768837 Msa0557400:intron 25.0%
!! AATAAAAAAGTCTAAAGCAC+CGG + chr4_1:55768843-55768862 Msa0557400:intron 25.0%
!! CTGTTCATTTATTCTACTTT+TGG - chr4_1:55768893-55768912 None:intergenic 25.0%
! GTAGAATAAATGAACAGTTT+AGG + chr4_1:55768896-55768915 Msa0557400:intron 25.0%
! ATTTACTCTTACTATTAGGA+GGG - chr4_1:55768989-55769008 None:intergenic 25.0%
! TATTTACTCTTACTATTAGG+AGG - chr4_1:55768990-55769009 None:intergenic 25.0%
! TATCTTAAATGTCAACAAAG+AGG + chr4_1:55769376-55769395 Msa0557400:intron 25.0%
! ATTCACATCATGTGAAATTT+AGG - chr4_1:55769649-55769668 None:intergenic 25.0%
! AATAAGCAGTTTACATCTAA+TGG + chr4_1:55769692-55769711 Msa0557400:intron 25.0%
!! GTTTACATCTAATGGATAAA+AGG + chr4_1:55769700-55769719 Msa0557400:intron 25.0%
! ATGTTGAGAAGACTATAAAA+AGG + chr4_1:55769795-55769814 Msa0557400:intron 25.0%
! TTTGACATAATGAGTATGAT+AGG - chr4_1:55769923-55769942 None:intergenic 25.0%
! GGCTAATTTCTATTTATTTC+TGG - chr4_1:55769989-55770008 None:intergenic 25.0%
! TTCCATTGCTGTAAAAAAAA+AGG - chr4_1:55770030-55770049 None:intergenic 25.0%
!!! TTTTTTTTTACAGCAATGGA+AGG + chr4_1:55770029-55770048 Msa0557400:intron 25.0%
! TAGTTAAATAGGTCTAAACT+TGG - chr4_1:55770092-55770111 None:intergenic 25.0%
! AATATAAAATCTCCACTTTG+AGG + chr4_1:55770201-55770220 Msa0557400:intron 25.0%
! ATTCAGCAATATAGTAGTAT+TGG - chr4_1:55770471-55770490 None:intergenic 25.0%
! TAATGAAACAAAGAGTGCAA+AGG + chr4_1:55767885-55767904 Msa0557400:CDS 30.0%
CTACATAAGAGGGAAATAAT+TGG - chr4_1:55768071-55768090 None:intergenic 30.0%
! TTTCAAAGTGTCAGAGAAAA+TGG + chr4_1:55768114-55768133 Msa0557400:CDS 30.0%
AAAGATGAAGAAAGAAGAAG+AGG + chr4_1:55768323-55768342 Msa0557400:CDS 30.0%
!! GAATCTCTAAAATCTTGTTG+TGG - chr4_1:55768411-55768430 None:intergenic 30.0%
!!! TTCTACTTTTGGAATCTCAT+TGG - chr4_1:55768882-55768901 None:intergenic 30.0%
GCATATTACTACAATTACAG+TGG + chr4_1:55768932-55768951 Msa0557400:intron 30.0%
! TGTTTTATGTAATCAGGTGA+CGG - chr4_1:55769191-55769210 None:intergenic 30.0%
AAGACATCTATTATGGTGAT+AGG - chr4_1:55769341-55769360 None:intergenic 30.0%
CAAATCGAAGACATCTATTA+TGG - chr4_1:55769348-55769367 None:intergenic 30.0%
!!! TTTTGACTTCACTATTTGAG+AGG + chr4_1:55769478-55769497 Msa0557400:intron 30.0%
TATCATATCAGGCATCTATA+AGG + chr4_1:55769568-55769587 Msa0557400:intron 30.0%
CTGGAAATATTATCTAGTCA+TGG - chr4_1:55769970-55769989 None:intergenic 30.0%
!!! TGCCTTTTTTTTTACAGCAA+TGG + chr4_1:55770025-55770044 Msa0557400:intron 30.0%
GAATATAAAAGTCCTCAAAG+TGG - chr4_1:55770216-55770235 None:intergenic 30.0%
GTCTCAAAAGTTACAAGAAT+GGG - chr4_1:55770314-55770333 None:intergenic 30.0%
AGTCTCAAAAGTTACAAGAA+TGG - chr4_1:55770315-55770334 None:intergenic 30.0%
! AAATGAATTTTCCTGCTGAA+AGG + chr4_1:55770360-55770379 Msa0557400:intron 30.0%
!! TGATTTTGGGAATTATTCCT+AGG - chr4_1:55770509-55770528 None:intergenic 30.0%
!!! CTGTTTTTGGAACTGATTTT+GGG - chr4_1:55770522-55770541 None:intergenic 30.0%
! CTACTTCATATTCCTGTTTT+TGG - chr4_1:55770535-55770554 None:intergenic 30.0%
!! CTCATGTATCCTCTTTTTCA+TGG + chr4_1:55767530-55767549 Msa0557400:CDS 35.0%
AATTGTGATGAACCATCAGA+CGG - chr4_1:55767676-55767695 None:intergenic 35.0%
GTTCATCACAATTTCCTTGT+GGG + chr4_1:55767683-55767702 Msa0557400:CDS 35.0%
ACATCCAAACTGTACATTAC+TGG - chr4_1:55767817-55767836 None:intergenic 35.0%
AGTTTGGATGTGAAAAGACA+TGG + chr4_1:55767826-55767845 Msa0557400:CDS 35.0%
CTTTCTGCATCTACATAAGA+GGG - chr4_1:55768081-55768100 None:intergenic 35.0%
ACTTTCTGCATCTACATAAG+AGG - chr4_1:55768082-55768101 None:intergenic 35.0%
TATGTAGATGCAGAAAGTTC+TGG + chr4_1:55768084-55768103 Msa0557400:CDS 35.0%
AAATGGTGTGAATCAATGTG+AGG + chr4_1:55768131-55768150 Msa0557400:CDS 35.0%
! ATGTGAGGAGTTTAAAGCAA+AGG + chr4_1:55768146-55768165 Msa0557400:CDS 35.0%
AAGCAAAGGTTAACAAAAGG+AGG + chr4_1:55768160-55768179 Msa0557400:CDS 35.0%
AGCAAAGGTTAACAAAAGGA+GGG + chr4_1:55768161-55768180 Msa0557400:CDS 35.0%
! AAGAGTACATTCTCCAAAGA+TGG + chr4_1:55768242-55768261 Msa0557400:CDS 35.0%
AGTCGAAATACGCAAAATGA+AGG + chr4_1:55768272-55768291 Msa0557400:CDS 35.0%
GAAAGAAGAAGAGGAAATTG+TGG + chr4_1:55768332-55768351 Msa0557400:CDS 35.0%
AACAACTCTTCCATTTCTTC+TGG - chr4_1:55768471-55768490 None:intergenic 35.0%
! CATTATCAAAGCATTTCGAC+AGG + chr4_1:55768533-55768552 Msa0557400:CDS 35.0%
TACTACAATTACAGTGGTCT+TGG + chr4_1:55768938-55768957 Msa0557400:intron 35.0%
! AATTACAGTGGTCTTGGATA+AGG + chr4_1:55768944-55768963 Msa0557400:intron 35.0%
TGGGACAATTAGTATACTGA+AGG + chr4_1:55769103-55769122 Msa0557400:intron 35.0%
GGGACAATTAGTATACTGAA+GGG + chr4_1:55769104-55769123 Msa0557400:intron 35.0%
! GTTTTATGTAATCAGGTGAC+GGG - chr4_1:55769190-55769209 None:intergenic 35.0%
AAAGAGGTAACACCAGTTAT+GGG - chr4_1:55769245-55769264 None:intergenic 35.0%
AAAAGAGGTAACACCAGTTA+TGG - chr4_1:55769246-55769265 None:intergenic 35.0%
AAAATGCCTATGAGAGCTTA+CGG + chr4_1:55769299-55769318 Msa0557400:intron 35.0%
CACATCATGTGAAATTTAGG+TGG - chr4_1:55769646-55769665 None:intergenic 35.0%
ATAAAAAGGTCCAAGTCTTG+TGG + chr4_1:55769809-55769828 Msa0557400:intron 35.0%
TGAAATCTTACCACAAGACT+TGG - chr4_1:55769822-55769841 None:intergenic 35.0%
AAACTTGGATTGCCTTTAGA+AGG - chr4_1:55770077-55770096 None:intergenic 35.0%
ACAAGGGGAAAGGTTTAATT+TGG + chr4_1:55770174-55770193 Msa0557400:intron 35.0%
! TTTTCCTGCTGAAAGGAATT+TGG + chr4_1:55770367-55770386 Msa0557400:intron 35.0%
! TTTTCCAAATTCCTTTCAGC+AGG - chr4_1:55770374-55770393 None:intergenic 35.0%
ATAGAGATTCAGTTCATCCT+AGG + chr4_1:55770489-55770508 Msa0557400:CDS 35.0%
!!! CCTGTTTTTGGAACTGATTT+TGG - chr4_1:55770523-55770542 None:intergenic 35.0%
CCAAAATCAGTTCCAAAAAC+AGG + chr4_1:55770520-55770539 Msa0557400:CDS 35.0%
GATACATGAGAGATGAAGGA+AGG - chr4_1:55767520-55767539 None:intergenic 40.0%
AGAGGATACATGAGAGATGA+AGG - chr4_1:55767524-55767543 None:intergenic 40.0%
CTTTGACAGCCATGAAAAAG+AGG - chr4_1:55767542-55767561 None:intergenic 40.0%
TTGTTGTCGTTGCATCAGAT+GGG - chr4_1:55767627-55767646 None:intergenic 40.0%
ATTGTTGTCGTTGCATCAGA+TGG - chr4_1:55767628-55767647 None:intergenic 40.0%
ATTGTGATGAACCATCAGAC+GGG - chr4_1:55767675-55767694 None:intergenic 40.0%
GGTTCATCACAATTTCCTTG+TGG + chr4_1:55767682-55767701 Msa0557400:CDS 40.0%
CAATTTCCTTGTGGGAATAG+AGG + chr4_1:55767691-55767710 Msa0557400:CDS 40.0%
!! AATAGAGGCAGGTTTTATGG+AGG + chr4_1:55767706-55767725 Msa0557400:CDS 40.0%
! GCATTCTGGAGATTGTCATT+TGG + chr4_1:55767742-55767761 Msa0557400:CDS 40.0%
GAAGCCAGTAATGTACAGTT+TGG + chr4_1:55767810-55767829 Msa0557400:CDS 40.0%
AGGAAGTTGAGTGCTTAAAG+AGG + chr4_1:55767905-55767924 Msa0557400:CDS 40.0%
GCTTAAAGAGGAGATATGAG+AGG + chr4_1:55767917-55767936 Msa0557400:CDS 40.0%
GAAATTACAGAATGCAGCAG+AGG + chr4_1:55767972-55767991 Msa0557400:CDS 40.0%
! TTGGAGCTCATCCTTTTCTA+AGG - chr4_1:55768031-55768050 None:intergenic 40.0%
! GGGTTCTTGGTGATTCAAAT+TGG - chr4_1:55768050-55768069 None:intergenic 40.0%
GAGGGAAATAATTGGGTTCT+TGG - chr4_1:55768063-55768082 None:intergenic 40.0%
ATGTGAGCAGAGAATTACAG+AGG + chr4_1:55768193-55768212 Msa0557400:CDS 40.0%
TGGAGAATGTACTCTTACCT+TGG - chr4_1:55768238-55768257 None:intergenic 40.0%
TCGACTTTAGTAGCCATCTT+TGG - chr4_1:55768258-55768277 None:intergenic 40.0%
AGAAGAAGAGGAAATTGTGG+AGG + chr4_1:55768335-55768354 Msa0557400:CDS 40.0%
ATTGTGGAGGAAACAGAAGA+AGG + chr4_1:55768348-55768367 Msa0557400:CDS 40.0%
TTGTGGAGGAAACAGAAGAA+GGG + chr4_1:55768349-55768368 Msa0557400:CDS 40.0%
! TGCAGTTGTGAAGTGTTCTT+TGG + chr4_1:55768383-55768402 Msa0557400:CDS 40.0%
! TCAAAGCATTTCGACAGGTA+TGG + chr4_1:55768538-55768557 Msa0557400:intron 40.0%
!! CATTGGCTTTCTCTTCTTAC+CGG - chr4_1:55768865-55768884 None:intergenic 40.0%
ACTATTAGGAGGGTTGGAAA+TGG - chr4_1:55768979-55768998 None:intergenic 40.0%
ACTCTTACTATTAGGAGGGT+TGG - chr4_1:55768985-55769004 None:intergenic 40.0%
CGTCTGCAAGAATAGGATAA+AGG - chr4_1:55769162-55769181 None:intergenic 40.0%
CAAGTCACTGTCTAACTACT+TGG + chr4_1:55769413-55769432 Msa0557400:intron 40.0%
CTTGGCAATAGCATACCAAA+TGG + chr4_1:55769431-55769450 Msa0557400:intron 40.0%
ACCAAATGGAACAACTCTTG+TGG + chr4_1:55769445-55769464 Msa0557400:intron 40.0%
ACCACAAGAGTTGTTCCATT+TGG - chr4_1:55769449-55769468 None:intergenic 40.0%
AATGGAACAACTCTTGTGGT+TGG + chr4_1:55769449-55769468 Msa0557400:intron 40.0%
! AAAATCTGCTATGACTTGGC+TGG - chr4_1:55769734-55769753 None:intergenic 40.0%
! AGCCAAGTCATAGCAGATTT+TGG + chr4_1:55769733-55769752 Msa0557400:intron 40.0%
CACCAAAATCTGCTATGACT+TGG - chr4_1:55769738-55769757 None:intergenic 40.0%
AGGCATGATTGAGTTAAGCA+TGG - chr4_1:55770010-55770029 None:intergenic 40.0%
GTGATCATCTCACAGTTGTT+AGG + chr4_1:55770051-55770070 Msa0557400:intron 40.0%
ACAGTTGTTAGGCCTTCTAA+AGG + chr4_1:55770062-55770081 Msa0557400:intron 40.0%
CTGCTGAAACATTCATGAGT+GGG + chr4_1:55770136-55770155 Msa0557400:intron 40.0%
GTAAGTGCAAAAGGTGACAA+GGG + chr4_1:55770158-55770177 Msa0557400:intron 40.0%
TAAGTGCAAAAGGTGACAAG+GGG + chr4_1:55770159-55770178 Msa0557400:intron 40.0%
TCTATTTGCATGAGGGTCTA+GGG - chr4_1:55770247-55770266 None:intergenic 40.0%
TGGGTAGTCTATTTGCATGA+GGG - chr4_1:55770254-55770273 None:intergenic 40.0%
ATGGGTAGTCTATTTGCATG+AGG - chr4_1:55770255-55770274 None:intergenic 40.0%
GAAGAAGCTGAAGCTGAAGA+AGG - chr4_1:55767484-55767503 None:intergenic 45.0%
! GGAGTTCTGCATCAGCTTTT+CGG - chr4_1:55767605-55767624 None:intergenic 45.0%
TGTTGTCGTTGCATCAGATG+GGG - chr4_1:55767626-55767645 None:intergenic 45.0%
TTGTGATGAACCATCAGACG+GGG - chr4_1:55767674-55767693 None:intergenic 45.0%
! GGGAATAGAGGCAGGTTTTA+TGG + chr4_1:55767703-55767722 Msa0557400:CDS 45.0%
! GATGCAGAAAGTTCTGGTCT+TGG + chr4_1:55768090-55768109 Msa0557400:CDS 45.0%
TGAGCAGAGAATTACAGAGG+AGG + chr4_1:55768196-55768215 Msa0557400:CDS 45.0%
GAAACCAAAGCAGAGTTCCA+AGG + chr4_1:55768218-55768237 Msa0557400:CDS 45.0%
CTTACCTTGGAACTCTGCTT+TGG - chr4_1:55768225-55768244 None:intergenic 45.0%
GATAAGCCAGCCAGAAGAAA+TGG + chr4_1:55768458-55768477 Msa0557400:CDS 45.0%
ACTCTTCCATTTCTTCTGGC+TGG - chr4_1:55768467-55768486 None:intergenic 45.0%
TAGGAGGGTTGGAAATGGTA+GGG - chr4_1:55768974-55768993 None:intergenic 45.0%
TTAGGAGGGTTGGAAATGGT+AGG - chr4_1:55768975-55768994 None:intergenic 45.0%
TGGGTCAGTCATGTTCTCTT+AGG - chr4_1:55769226-55769245 None:intergenic 45.0%
GAACATGACTGACCCATAAC+TGG + chr4_1:55769230-55769249 Msa0557400:intron 45.0%
GCTGCTGAAACATTCATGAG+TGG + chr4_1:55770135-55770154 Msa0557400:intron 45.0%
CATGAGTGGGTAAGTGCAAA+AGG + chr4_1:55770149-55770168 Msa0557400:intron 45.0%
GGTAAGTGCAAAAGGTGACA+AGG + chr4_1:55770157-55770176 Msa0557400:intron 45.0%
GTCTATTTGCATGAGGGTCT+AGG - chr4_1:55770248-55770267 None:intergenic 45.0%
!!! TAGTTTTTTTTTTTTTTTTT+TGG - chr4_1:55769055-55769074 None:intergenic 5.0%
TTCCTTGTGGGAATAGAGGC+AGG + chr4_1:55767695-55767714 Msa0557400:CDS 50.0%
AACCTGCCTCTATTCCCACA+AGG - chr4_1:55767700-55767719 None:intergenic 50.0%
!! AGAGGCAGGTTTTATGGAGG+TGG + chr4_1:55767709-55767728 Msa0557400:CDS 50.0%
!! CAGGTTTTATGGAGGTGGTG+AGG + chr4_1:55767714-55767733 Msa0557400:CDS 50.0%
GTGGTGAGGATGATGCATTC+TGG + chr4_1:55767728-55767747 Msa0557400:CDS 50.0%
GGTCTTGCGTCTGCAAGAAT+AGG - chr4_1:55769169-55769188 None:intergenic 50.0%
ATGCCTATGAGAGCTTACGG+TGG + chr4_1:55769302-55769321 Msa0557400:intron 50.0%
TGCCTATGAGAGCTTACGGT+GGG + chr4_1:55769303-55769322 Msa0557400:intron 50.0%
ATCCCACCGTAAGCTCTCAT+AGG - chr4_1:55769308-55769327 None:intergenic 50.0%
GCAAAAGGTGACAAGGGGAA+AGG + chr4_1:55770164-55770183 Msa0557400:intron 50.0%
!! TCAGACGGGGAGTTCTGAGT+GGG - chr4_1:55767661-55767680 None:intergenic 55.0%
!! ATCAGACGGGGAGTTCTGAG+TGG - chr4_1:55767662-55767681 None:intergenic 55.0%
ACTCAGAACTCCCCGTCTGA+TGG + chr4_1:55767661-55767680 Msa0557400:CDS 55.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 55767460 55770559 55767460 ID=Msa0557400;Name=Msa0557400
chr4_1 mRNA 55767460 55770559 55767460 ID=Msa0557400-mRNA-1;Parent=Msa0557400;Name=Msa0557400-mRNA-1;_AED=0.10;_eAED=0.15;_QI=0|0|0|1|1|1|2|0|416
chr4_1 exon 55767460 55768554 55767460 ID=Msa0557400-mRNA-1:exon:16678;Parent=Msa0557400-mRNA-1
chr4_1 exon 55770404 55770559 55770404 ID=Msa0557400-mRNA-1:exon:16679;Parent=Msa0557400-mRNA-1
chr4_1 CDS 55767460 55768554 55767460 ID=Msa0557400-mRNA-1:cds;Parent=Msa0557400-mRNA-1
chr4_1 CDS 55770404 55770559 55770404 ID=Msa0557400-mRNA-1:cds;Parent=Msa0557400-mRNA-1
Gene Sequence

>Msa0557400

ATGAAGTGGAGTGGAAGAAAACCTTCTTCAGCTTCAGCTTCTTCTTCTTCTTCTAAGCCTTCCTTCATCTCTCATGTATCCTCTTTTTCATGGCTGTCAAAGTTCAAGCAAATGAAAATCAACTCAGACTCAGAAGCAAAATCCGAAAAGCTGATGCAGAACTCCCCATCTGATGCAACGACAACAATCAAGCATTATCCCACTCAGAACTCCCCGTCTGATGGTTCATCACAATTTCCTTGTGGGAATAGAGGCAGGTTTTATGGAGGTGGTGAGGATGATGCATTCTGGAGATTGTCATTTGGTGAAGAGTTTACTAATGACCAAAAGAAGAAAAGTAAAGATATTCTGAAGCCAGTAATGTACAGTTTGGATGTGAAAAGACATGGAAGAAGAAGAAGAAGAAGAGAAGATAGAAAATTGCTTAATGAAACAAAGAGTGCAAAGGAAGTTGAGTGCTTAAAGAGGAGATATGAGAGGAAAGCACAAAGAGCTTTACAAGAGCAGCTCTTGAAATTACAGAATGCAGCAGAGGAAGCTGAATTTGTTGCATCAAGTAAATGTAAATCCTTAGAAAAGGATGAGCTCCAATTTGAATCACCAAGAACCCAATTATTTCCCTCTTATGTAGATGCAGAAAGTTCTGGTCTTGGATTTCAAAGTGTCAGAGAAAATGGTGTGAATCAATGTGAGGAGTTTAAAGCAAAGGTTAACAAAAGGAGGGAATCAGTTCATGTGAGCAGAGAATTACAGAGGAGGAAACCAAAGCAGAGTTCCAAGGTAAGAGTACATTCTCCAAAGATGGCTACTAAAGTCGAAATACGCAAAATGAAGGCGATAGAAGACAAAAAGAAAGCAAAACTAAAGATGAAGAAAGAAGAAGAGGAAATTGTGGAGGAAACAGAAGAAGGGTTAGATAGTTTTGCAGTTGTGAAGTGTTCTTTGGATCCACAACAAGATTTTAGAGATTCAATGATTGAGATGATCAAAGAGAAAGAGATAAGCCAGCCAGAAGAAATGGAAGAGTTGTTAGCATGCTATTTGAGTTTGAATTCCAATGAATACCATGATATCATTATCAAAGCATTTCGACAGACAACAAATGAAGCTCGTTTAAAAGAGATTAGTATGCTGATTAATATCGACAAAACAAGTAAAGCCAATACTACTATATTGCTGAATAGAGATTCAGTTCATCCTAGGAATAATTCCCAAAATCAGTTCCAAAAACAGGAATATGAAGTAGCATAA
Protein sequence

>Msa0557400

MKWSGRKPSSASASSSSSKPSFISHVSSFSWLSKFKQMKINSDSEAKSEKLMQNSPSDATTTIKHYPTQNSPSDGSSQFPCGNRGRFYGGGEDDAFWRLSFGEEFTNDQKKKSKDILKPVMYSLDVKRHGRRRRRREDRKLLNETKSAKEVECLKRRYERKAQRALQEQLLKLQNAAEEAEFVASSKCKSLEKDELQFESPRTQLFPSYVDAESSGLGFQSVRENGVNQCEEFKAKVNKRRESVHVSRELQRRKPKQSSKVRVHSPKMATKVEIRKMKAIEDKKKAKLKMKKEEEEIVEETEEGLDSFAVVKCSLDPQQDFRDSMIEMIKEKEISQPEEMEELLACYLSLNSNEYHDIIIKAFRQTTNEARLKEISMLINIDKTSKANTTILLNRDSVHPRNNSQNQFQKQEYEVA*