AlfalfaGEDB | Msa0558580

M. sativa cultivar zhongmu-4 / Msa0558580

KEGG: K09422
GO: GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031326 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0558580	QSD99742.1	88.643	361	37	2	1	361	1	357	0.0	605

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0558580	sp\|Q9LDR8\|MY102_ARATH	54.424	373	134	11	1	361	1	349	3.70e-115	341

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0558580	A0A896WD03	88.643	361	37	2	1	361	1	357	0.0	605

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TFs/TRs:

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Gene ID	Type	Classification
Msa0558580	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0558580	Msa0696320	0.893872	4.194235e-75	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0558580	MtrunA17_Chr4g0048511	86.501	363	42	4	1	361	1	358	0.0	604
Msa0558580	MtrunA17_Chr2g0281071	55.405	370	131	11	1	361	1	345	2.59e-100	300
Msa0558580	MtrunA17_Chr2g0323411	73.889	180	39	5	1	174	1	178	5.23e-89	271
Msa0558580	MtrunA17_Chr3g0115821	74.627	134	33	1	1	134	1	133	1.98e-75	236
Msa0558580	MtrunA17_Chr4g0039971	69.595	148	44	1	1	148	2	148	1.16e-74	234
Msa0558580	MtrunA17_Chr7g0217741	74.627	134	33	1	1	134	1	133	2.32e-74	233
Msa0558580	MtrunA17_Chr6g0453471	69.079	152	46	1	1	152	1	151	8.98e-74	233
Msa0558580	MtrunA17_Chr5g0410961	65.517	174	50	3	1	165	1	173	2.51e-73	231
Msa0558580	MtrunA17_Chr4g0042671	75.000	132	32	1	1	132	1	131	5.80e-73	232
Msa0558580	MtrunA17_Chr5g0395281	60.345	174	59	3	1	165	1	173	4.23e-69	219

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0558580	AT4G21440.1	54.424	373	134	11	1	361	1	349	3.76e-116	341
Msa0558580	AT4G05100.1	51.482	371	114	8	1	355	1	321	5.18e-102	304
Msa0558580	AT5G54230.1	64.115	209	69	4	13	218	12	217	5.78e-84	258
Msa0558580	AT4G28110.1	47.922	361	108	11	1	361	1	281	1.31e-83	256
Msa0558580	AT5G16770.4	61.140	193	67	3	1	188	1	190	2.12e-79	246
Msa0558580	AT5G16770.3	61.140	193	67	3	1	188	1	190	2.12e-79	246
Msa0558580	AT5G16770.1	61.140	193	67	3	1	188	1	190	4.93e-79	246
Msa0558580	AT5G16770.2	61.140	193	67	3	1	188	1	190	4.93e-79	246
Msa0558580	AT3G02940.2	77.941	136	29	1	1	136	1	135	4.40e-77	240
Msa0558580	AT3G02940.1	77.941	136	29	1	1	136	1	135	1.12e-76	239

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Find 70 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCCTGAGATTCTAAAGT+TGG	0.448442	4_1:+57483305	Msa0558580:CDS
AACGGTGTAGGACTTGATGA+AGG	0.711108	4_1:-57483756	None:intergenic
AACTATATCACATACTACCA+TGG	0.574663	4_1:+57482476	Msa0558580:exon
AAGAATAATGGCCTCAAAAG+AGG	0.510219	4_1:+57482519	Msa0558580:CDS
AAGAGGACCATGGACACTAG+AGG	0.651258	4_1:+57482536	Msa0558580:CDS
AAGATAGGTCCAAAAGATCA+AGG	0.542013	4_1:-57483203	None:intergenic
AAGCCAACTTTAGAATCTCA+GGG	0.582480	4_1:-57483308	None:intergenic
AATCGTCCTCTCTTTATATC+CGG	0.517026	4_1:-57482785	None:intergenic
AATGAGTTCATGTAATATTT+TGG	0.185552	4_1:+57483885	Msa0558580:exon
AATGCTATGCAGCTGAATTA+TGG	0.360495	4_1:-57482828	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GATATGATATGTCTGAAAAG+TGG	-	chr4_1:57482379-57482398	None:intergenic	30.0%
AACTATATCACATACTACCA+TGG	+	chr4_1:57482476-57482495	Msa0558580:exon	30.0%
ACTATATCACATACTACCAT+GGG	+	chr4_1:57482477-57482496	Msa0558580:exon	30.0%
TCAATTTAACTAGACACATG+TGG	+	chr4_1:57482927-57482946	Msa0558580:intron	30.0%
ATTATTGGAACACTCACATT+AGG	+	chr4_1:57483133-57483152	Msa0558580:CDS	30.0%
GAATTATTATGATCACAAGG+TGG	-	chr4_1:57483438-57483457	None:intergenic	30.0%
ATGTTGTTGGAAGTCAAATT+CGG	-	chr4_1:57483523-57483542	None:intergenic	30.0%
TTGAAGTTTCTGATATGTGA+GGG	-	chr4_1:57483680-57483699	None:intergenic	30.0%
GATATGATATGTCTGAAAAG+TGG	-	chr4_1:57482379-57482398	None:intergenic	30.0%
AACTATATCACATACTACCA+TGG	+	chr4_1:57482476-57482495	Msa0558580:exon	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	57482366	57483932	57482366	ID=Msa0558580;Name=Msa0558580
chr4_1	mRNA	57482366	57483932	57482366	ID=Msa0558580-mRNA-1;Parent=Msa0558580;Name=Msa0558580-mRNA-1;_AED=0.07;_eAED=0.07;_QI=129\|1\|1\|1\|1\|1\|3\|33\|361
chr4_1	exon	57482366	57482630	57482366	ID=Msa0558580-mRNA-1:exon:17378;Parent=Msa0558580-mRNA-1
chr4_1	exon	57482732	57482861	57482732	ID=Msa0558580-mRNA-1:exon:17379;Parent=Msa0558580-mRNA-1
chr4_1	exon	57483080	57483932	57483080	ID=Msa0558580-mRNA-1:exon:17380;Parent=Msa0558580-mRNA-1
chr4_1	five_prime_UTR	57482366	57482494	57482366	ID=Msa0558580-mRNA-1:five_prime_utr;Parent=Msa0558580-mRNA-1
chr4_1	CDS	57482495	57482630	57482495	ID=Msa0558580-mRNA-1:cds;Parent=Msa0558580-mRNA-1
chr4_1	CDS	57482732	57482861	57482732	ID=Msa0558580-mRNA-1:cds;Parent=Msa0558580-mRNA-1
chr4_1	CDS	57483080	57483899	57483080	ID=Msa0558580-mRNA-1:cds;Parent=Msa0558580-mRNA-1
chr4_1	three_prime_UTR	57483900	57483932	57483900	ID=Msa0558580-mRNA-1:three_prime_utr;Parent=Msa0558580-mRNA-1

Gene Sequence

>Msa0558580

ATGGGAAGAGCACCTTGTTGTGAAAAGAATAATGGCCTCAAAAGAGGACCATGGACACTAGAGGAAGACCAAAAACTTATAGATTATATTCAAAAACATGGCTATGGCAACTGGAGATTACTCCCAAAGAATGCTGGATTACAAAGATGTGGAAAGAGTTGTCGTCTCCGTTGGACAAACTATCTCCGACCGGATATAAAGAGAGGACGATTCTCTTTTGAAGAAGAAGAGACCATAATTCAGCTGCATAGCATTCTTGGCAACAAGTGGTCTTCAATTGCTTCTAGGTTACCAGGAAGAACAGACAATGAAATCAAGAATTATTGGAACACTCACATTAGGAAAAAACTCTTAAGAATGGGAATTGATCCTGTGACACACAATCCACGCCTTGATCTTTTGGACCTATCTTCTATCCTAAATTCATCTCTCTATGCCTCTACCTCATCACAAATGAACATCCAAAGGCTAATTGGTACACAATCATTAGTAAACCCTGAGATTCTAAAGTTGGCTTCATCACTCTTCTCTTCTCAAAATGGACAAGAGAATTACCAAATTAGTCAAAACCAACAAATTAGCTCACACTTGGATCAAGAGGCTTGCACCATGTTGTTGAATCCACCTTGTGATCATAATAATTCAACGCCTTTCACTCAAACACACTTGGTTGAATCTAATGAGAATCTATGCTCATCATTTTTACCCGAATTTGACTTCCAACAACATCGTGAAAATGTTCAATTAAGTGATTTGTATTGCAATGGAATTGCTTCAAGTAATGTAACAGAGGATTTTGTTCATCAATTACCATGCTATAACTACTCAAGTTCTGATTATCATGCAAATGATTTAATTATGGTCCCTCACATATCAGAAACTTCAACCTTTCATTGCAATAGCAACAACCAGAATTTCAATTTCGCTTCAGTTCTATCCACACCTTCATCAAGTCCTACACCGTTGCATTCGAATTCGACGAATATCAATGGAAGCAACATTGAAGATGAGACAGAGAACTATGTTAGCAGCAACATATTTGAATTTCATATCTCAAATATCTTAGGTGTGAATGAGTTCATGTAA

Protein sequence

>Msa0558580

MGRAPCCEKNNGLKRGPWTLEEDQKLIDYIQKHGYGNWRLLPKNAGLQRCGKSCRLRWTNYLRPDIKRGRFSFEEEETIIQLHSILGNKWSSIASRLPGRTDNEIKNYWNTHIRKKLLRMGIDPVTHNPRLDLLDLSSILNSSLYASTSSQMNIQRLIGTQSLVNPEILKLASSLFSSQNGQENYQISQNQQISSHLDQEACTMLLNPPCDHNNSTPFTQTHLVESNENLCSSFLPEFDFQQHRENVQLSDLYCNGIASSNVTEDFVHQLPCYNYSSSDYHANDLIMVPHISETSTFHCNSNNQNFNFASVLSTPSSSPTPLHSNSTNINGSNIEDETENYVSSNIFEFHISNILGVNEFM*