AlfalfaGEDB | Msa0696320

M. sativa cultivar zhongmu-4 / Msa0696320

KEGG: K09422
GO: GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031326 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0696320	QSD99742.1	88.920	361	36	2	1	361	1	357	0.0	606

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0696320	sp\|Q9LDR8\|MY102_ARATH	54.155	373	135	11	1	361	1	349	2.69e-114	339

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0696320	A0A896WD03	88.920	361	36	2	1	361	1	357	0.0	606

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TFs/TRs:

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Gene ID	Type	Classification
Msa0696320	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0558580	Msa0696320	0.893872	4.194235e-75	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0696320	MtrunA17_Chr4g0048511	86.226	363	43	4	1	361	1	358	0.0	602
Msa0696320	MtrunA17_Chr2g0281071	55.135	370	132	11	1	361	1	345	1.44e-99	298
Msa0696320	MtrunA17_Chr2g0323411	73.333	180	40	5	1	174	1	178	2.17e-88	269
Msa0696320	MtrunA17_Chr4g0039971	70.270	148	43	1	1	148	2	148	2.63e-75	236
Msa0696320	MtrunA17_Chr3g0115821	74.627	134	33	1	1	134	1	133	3.29e-75	236
Msa0696320	MtrunA17_Chr7g0217741	74.627	134	33	1	1	134	1	133	2.79e-74	232
Msa0696320	MtrunA17_Chr6g0453471	62.011	179	64	2	1	176	1	178	1.14e-73	232
Msa0696320	MtrunA17_Chr5g0410961	65.517	174	50	3	1	165	1	173	3.33e-73	231
Msa0696320	MtrunA17_Chr4g0042671	75.000	132	32	1	1	132	1	131	8.17e-73	231
Msa0696320	MtrunA17_Chr5g0395281	60.345	174	59	3	1	165	1	173	6.04e-69	218

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0696320	AT4G21440.1	54.155	373	135	11	1	361	1	349	2.73e-115	339
Msa0696320	AT4G05100.1	51.213	371	115	8	1	355	1	321	2.45e-101	302
Msa0696320	AT5G54230.1	64.115	209	69	4	13	218	12	217	3.18e-84	258
Msa0696320	AT4G28110.1	47.645	361	109	11	1	361	1	281	5.56e-83	254
Msa0696320	AT5G16770.4	61.140	193	67	3	1	188	1	190	2.29e-79	246
Msa0696320	AT5G16770.3	61.140	193	67	3	1	188	1	190	2.29e-79	246
Msa0696320	AT5G16770.1	61.140	193	67	3	1	188	1	190	4.82e-79	246
Msa0696320	AT5G16770.2	61.140	193	67	3	1	188	1	190	4.82e-79	246
Msa0696320	AT3G02940.2	77.941	136	29	1	1	136	1	135	3.70e-77	240
Msa0696320	AT3G02940.1	77.941	136	29	1	1	136	1	135	9.69e-77	239

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Find 69 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCCTGAGATTCTAAAGT+TGG	0.448442	4_4:+66294500	Msa0696320:CDS
AACGGTGTAGGACTTGATGA+AGG	0.711108	4_4:-66294951	None:intergenic
AACTATAGCACATACTACCA+TGG	0.595506	4_4:+66293664	Msa0696320:exon
AAGAATAATGGCCTCAAAAG+AGG	0.510219	4_4:+66293707	Msa0696320:CDS
AAGAGGACCATGGACACTAG+AGG	0.651258	4_4:+66293724	Msa0696320:CDS
AAGATAGGTCCAAAAGATCA+AGG	0.542013	4_4:-66294398	None:intergenic
AAGCCAACTTTAGAATCTCA+GGG	0.582480	4_4:-66294503	None:intergenic
AATGAGTTCATGTAATATTT+TGG	0.185552	4_4:+66295080	Msa0696320:exon
AATGCTATGCAGCTGAATTA+TGG	0.360495	4_4:-66294027	None:intergenic
AATTGTCCTCTCTTTATATC+CGG	0.459653	4_4:-66293984	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GATATGATATGTCTGAAAAG+TGG	-	chr4_4:66293567-66293586	None:intergenic	30.0%
AATTGTCCTCTCTTTATATC+CGG	-	chr4_4:66293987-66294006	None:intergenic	30.0%
TCAATTTAACTAGACACATG+TGG	+	chr4_4:66294126-66294145	Msa0696320:intron	30.0%
ATTATTGGAACACTCACATT+AGG	+	chr4_4:66294328-66294347	Msa0696320:CDS	30.0%
GAATTATTATGATCACAAGG+TGG	-	chr4_4:66294633-66294652	None:intergenic	30.0%
ATGTTGTTGGAAGTCAAATT+CGG	-	chr4_4:66294718-66294737	None:intergenic	30.0%
TTGAAGTTTCTGATATGTGA+GGG	-	chr4_4:66294875-66294894	None:intergenic	30.0%
GATATGATATGTCTGAAAAG+TGG	-	chr4_4:66293567-66293586	None:intergenic	30.0%
AATTGTCCTCTCTTTATATC+CGG	-	chr4_4:66293987-66294006	None:intergenic	30.0%
TCAATTTAACTAGACACATG+TGG	+	chr4_4:66294126-66294145	Msa0696320:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_4	gene	66293554	66295127	66293554	ID=Msa0696320;Name=Msa0696320
chr4_4	mRNA	66293554	66295127	66293554	ID=Msa0696320-mRNA-1;Parent=Msa0696320;Name=Msa0696320-mRNA-1;_AED=0.08;_eAED=0.08;_QI=129\|1\|1\|1\|1\|1\|3\|33\|361
chr4_4	exon	66293554	66293818	66293554	ID=Msa0696320-mRNA-1:exon:19237;Parent=Msa0696320-mRNA-1
chr4_4	exon	66293931	66294060	66293931	ID=Msa0696320-mRNA-1:exon:19238;Parent=Msa0696320-mRNA-1
chr4_4	exon	66294275	66295127	66294275	ID=Msa0696320-mRNA-1:exon:19239;Parent=Msa0696320-mRNA-1
chr4_4	five_prime_UTR	66293554	66293682	66293554	ID=Msa0696320-mRNA-1:five_prime_utr;Parent=Msa0696320-mRNA-1
chr4_4	CDS	66293683	66293818	66293683	ID=Msa0696320-mRNA-1:cds;Parent=Msa0696320-mRNA-1
chr4_4	CDS	66293931	66294060	66293931	ID=Msa0696320-mRNA-1:cds;Parent=Msa0696320-mRNA-1
chr4_4	CDS	66294275	66295094	66294275	ID=Msa0696320-mRNA-1:cds;Parent=Msa0696320-mRNA-1
chr4_4	three_prime_UTR	66295095	66295127	66295095	ID=Msa0696320-mRNA-1:three_prime_utr;Parent=Msa0696320-mRNA-1

Gene Sequence

>Msa0696320

ATGGGAAGAGCACCTTGTTGTGAAAAGAATAATGGCCTCAAAAGAGGACCATGGACACTAGAGGAAGACCAAAAACTTATAGATTATATTCAAAAACATGGCTATGGCAACTGGAGATTACTCCCAAAGAATGCTGGATTACAAAGATGTGGAAAGAGTTGTCGTCTCCGTTGGACAAACTATCTCCGACCGGATATAAAGAGAGGACAATTCTCTTTTGAAGAAGAAGAGACCATAATTCAGCTGCATAGCATTCTTGGCAACAAGTGGTCTTCAATTGCTTCTAGGTTACCAGGAAGAACAGACAATGAAATCAAGAATTATTGGAACACTCACATTAGGAAAAAACTCTTAAGAATGGGAATTGATCCTGTGACACACAATCCACGCCTTGATCTTTTGGACCTATCTTCTATCCTAAATTCATCTCTCTATGCCTCTACCTCATCACAAATGAACATCCAAAGGCTAATTGGTACACAATCATTAGTAAACCCTGAGATTCTAAAGTTGGCTTCATCACTCTTCTCTTCTCAAAATGGACAAGAGAATTACCAAATTAGTCAAAACCAACAAATTAGCTCACACTTGGATCAAGAGGCTTGCACCATGTTGTTGAATCCACCTTGTGATCATAATAATTCAACGCCTTTCACTCAAACACACTTGGTTGAATCTAATGAGAATCTATGCTCATCATTTTTACCCGAATTTGACTTCCAACAACATCGTGAAAATGTTCAATTAAGTGATTTGTATTGCAATGGAATTGCTTCAAGTAATGTAACAGAGGATTTTGTTCATCAATTACCATGCTATAACTACTCAAGTTCTGATTATCATGCAAATGATTTAATTATGGTCCCTCACATATCAGAAACTTCAACCTTTCATTGCAATAGCAACAACCAGAATTTCAATTTCGCTTCAGTTCTATCCACACCTTCATCAAGTCCTACACCGTTGCATTCGAATTCGACGAATATCAATGGAAGCAACATTGAAGATGAGACAGAGAACTATGTTAGCAGCAACATATTTGAATTTCATATCTCAAATATCTTAGGTGTGAATGAGTTCATGTAA

Protein sequence

>Msa0696320

MGRAPCCEKNNGLKRGPWTLEEDQKLIDYIQKHGYGNWRLLPKNAGLQRCGKSCRLRWTNYLRPDIKRGQFSFEEEETIIQLHSILGNKWSSIASRLPGRTDNEIKNYWNTHIRKKLLRMGIDPVTHNPRLDLLDLSSILNSSLYASTSSQMNIQRLIGTQSLVNPEILKLASSLFSSQNGQENYQISQNQQISSHLDQEACTMLLNPPCDHNNSTPFTQTHLVESNENLCSSFLPEFDFQQHRENVQLSDLYCNGIASSNVTEDFVHQLPCYNYSSSDYHANDLIMVPHISETSTFHCNSNNQNFNFASVLSTPSSSPTPLHSNSTNINGSNIEDETENYVSSNIFEFHISNILGVNEFM*