AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0562910


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0562910 MtrunA17_Chr8g0376721 93.548 248 9 1 1 241 1 248 1.70e-173 477
Msa0562910 MtrunA17_Chr2g0285421 69.196 224 67 2 2 224 1 223 3.51e-114 327
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 32 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATTCAGGCCTTTGCTTAA+AGG 0.202937 4_1:+63173413 Msa0562910:CDS
TAAATCCAGATTATAGAAAT+TGG 0.211714 4_1:+63173505 Msa0562910:CDS
ATCATACTTCATCATGTTAA+TGG 0.223361 4_1:-63173553 None:intergenic
ATGCAATCCTTGCAATATTT+AGG 0.247387 4_1:+63173364 Msa0562910:CDS
ATCATCCAATTTCTATAATC+TGG 0.269076 4_1:-63173510 None:intergenic
AATTCAGGCCTTTGCTTAAA+GGG 0.281424 4_1:+63173414 Msa0562910:CDS
CAAGAGTATCCATGGAAATA+TGG 0.330050 4_1:+63172602 Msa0562910:CDS
TTGCATAGCACAAATCATTA+AGG 0.358283 4_1:-63173347 None:intergenic
ATTTGTGACTATTGAGTTCA+TGG 0.370041 4_1:-63173316 None:intergenic
ACATGAATGCATACAACATT+AGG 0.391046 4_1:-63173447 None:intergenic
TGTACTGATACTAGATCATA+TGG 0.393061 4_1:+63172729 Msa0562910:intron
TCCTCACGTGCTGAGAATCT+TGG 0.396867 4_1:+63172558 Msa0562910:CDS
TTTCGAGTGGTATATGTTAG+TGG 0.405631 4_1:+63173579 Msa0562910:CDS
GTTTGATTTCATGAATAAGA+TGG 0.454595 4_1:-63172530 None:intergenic
TGATACTAGATCATATGGTT+TGG 0.460097 4_1:+63172734 Msa0562910:intron
AAAACGGTCTTATGATGAAA+AGG 0.477023 4_1:+63172766 Msa0562910:CDS
ATGGAGAACCGTTCAAGATA+AGG 0.494548 4_1:-63173534 None:intergenic
TATTTCCTCAATGCATATGC+TGG 0.506807 4_1:+63173240 Msa0562910:CDS
GGATGATTCCTTATCTTGAA+CGG 0.529425 4_1:+63173526 Msa0562910:CDS
GCTAATGCATGTAACAAGAA+TGG 0.535827 4_1:+63173200 Msa0562910:CDS
AGATCATATGGTTTGGAGCA+TGG 0.539450 4_1:+63172741 Msa0562910:intron
ATTCTCCAGCATATGCATTG+AGG 0.539548 4_1:-63173245 None:intergenic
TGGTCAACAATTACTACAGT+TGG 0.545716 4_1:-63172510 None:intergenic
TGTAACAAGAATGGCTGTCT+TGG 0.548408 4_1:+63173209 Msa0562910:CDS
TCTATACTCAAGAGTATCCA+TGG 0.558936 4_1:+63172594 Msa0562910:CDS
ATATATATACCATATTTCCA+TGG 0.601472 4_1:-63172611 None:intergenic
AACGTGACCTAAATATTGCA+AGG 0.604286 4_1:-63173371 None:intergenic
AATGAAGAAGAGAGTAGACA+TGG 0.628824 4_1:+63173633 Msa0562910:CDS
TCTTCTTCATTATAATCACA+AGG 0.636274 4_1:-63173621 None:intergenic
ACCAAGATTCTCAGCACGTG+AGG 0.657985 4_1:-63172559 None:intergenic
TATGCTGGAGAATCAAGAGA+TGG 0.661834 4_1:+63173255 Msa0562910:CDS
ATGTTGTATGCATTCATGTG+TGG 0.683428 4_1:+63173451 Msa0562910:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAGATATTCTGTAATATT+AGG - chr4_1:63173120-63173139 None:intergenic 15.0%
!! TATAGATATTCTGTAATATT+AGG - chr4_1:63173120-63173139 None:intergenic 15.0%
!! ATATATATACCATATTTCCA+TGG - chr4_1:63172614-63172633 None:intergenic 20.0%
!! AAAACTAGAGAAATTAAAGA+TGG - chr4_1:63172639-63172658 None:intergenic 20.0%
!!! GTTTAAGTTTTCTTGTATTT+TGG + chr4_1:63172686-63172705 Msa0562910:intron 20.0%
!!! GAAAGAATATTAATTTTCGT+TGG - chr4_1:63173029-63173048 None:intergenic 20.0%
!!! TCAACTATAAATTTTAGTAC+AGG + chr4_1:63173282-63173301 Msa0562910:CDS 20.0%
!! TAAATCCAGATTATAGAAAT+TGG + chr4_1:63173505-63173524 Msa0562910:CDS 20.0%
!! ATATATATACCATATTTCCA+TGG - chr4_1:63172614-63172633 None:intergenic 20.0%
!! AAAACTAGAGAAATTAAAGA+TGG - chr4_1:63172639-63172658 None:intergenic 20.0%
!!! GTTTAAGTTTTCTTGTATTT+TGG + chr4_1:63172686-63172705 Msa0562910:intron 20.0%
!!! GAAAGAATATTAATTTTCGT+TGG - chr4_1:63173029-63173048 None:intergenic 20.0%
!!! TCAACTATAAATTTTAGTAC+AGG + chr4_1:63173282-63173301 Msa0562910:CDS 20.0%
!! TAAATCCAGATTATAGAAAT+TGG + chr4_1:63173505-63173524 Msa0562910:CDS 20.0%
!! GTTTGATTTCATGAATAAGA+TGG - chr4_1:63172533-63172552 None:intergenic 25.0%
! TAAACAGTATACTTAGAAGT+TGG - chr4_1:63172671-63172690 None:intergenic 25.0%
! AAAGTCTACTTTAAACAAGA+AGG - chr4_1:63172854-63172873 None:intergenic 25.0%
!!! TGAGGAATGTTACTTATTTT+TGG + chr4_1:63172896-63172915 Msa0562910:intron 25.0%
! ATCATCCAATTTCTATAATC+TGG - chr4_1:63173513-63173532 None:intergenic 25.0%
! ATCATACTTCATCATGTTAA+TGG - chr4_1:63173556-63173575 None:intergenic 25.0%
! TCTTCTTCATTATAATCACA+AGG - chr4_1:63173624-63173643 None:intergenic 25.0%
!! GTTTGATTTCATGAATAAGA+TGG - chr4_1:63172533-63172552 None:intergenic 25.0%
! TAAACAGTATACTTAGAAGT+TGG - chr4_1:63172671-63172690 None:intergenic 25.0%
! AAAGTCTACTTTAAACAAGA+AGG - chr4_1:63172854-63172873 None:intergenic 25.0%
!!! TGAGGAATGTTACTTATTTT+TGG + chr4_1:63172896-63172915 Msa0562910:intron 25.0%
! ATCATCCAATTTCTATAATC+TGG - chr4_1:63173513-63173532 None:intergenic 25.0%
! ATCATACTTCATCATGTTAA+TGG - chr4_1:63173556-63173575 None:intergenic 25.0%
! TCTTCTTCATTATAATCACA+AGG - chr4_1:63173624-63173643 None:intergenic 25.0%
!! AGATTTTAGCAACAAGGATT+TGG + chr4_1:63172464-63172483 Msa0562910:CDS 30.0%
! TGTACTGATACTAGATCATA+TGG + chr4_1:63172729-63172748 Msa0562910:intron 30.0%
TGATACTAGATCATATGGTT+TGG + chr4_1:63172734-63172753 Msa0562910:intron 30.0%
! AAAACGGTCTTATGATGAAA+AGG + chr4_1:63172766-63172785 Msa0562910:CDS 30.0%
TAAATGTGAAGTAGTAGTGA+GGG - chr4_1:63172823-63172842 None:intergenic 30.0%
ATAAATGTGAAGTAGTAGTG+AGG - chr4_1:63172824-63172843 None:intergenic 30.0%
!!! GTTACTTATTTTTGGTCATG+AGG + chr4_1:63172904-63172923 Msa0562910:intron 30.0%
TCCCTCATATTAAGATGTTA+GGG - chr4_1:63172958-63172977 None:intergenic 30.0%
AAAATGTCCATAAAGAGATG+TGG - chr4_1:63172988-63173007 None:intergenic 30.0%
ATTTGTGACTATTGAGTTCA+TGG - chr4_1:63173319-63173338 None:intergenic 30.0%
TTGCATAGCACAAATCATTA+AGG - chr4_1:63173350-63173369 None:intergenic 30.0%
ATGCAATCCTTGCAATATTT+AGG + chr4_1:63173364-63173383 Msa0562910:CDS 30.0%
! TGAATTTTTGCCCTCTATTT+GGG - chr4_1:63173400-63173419 None:intergenic 30.0%
ACATGAATGCATACAACATT+AGG - chr4_1:63173450-63173469 None:intergenic 30.0%
!!! GAAGTATGATATTTTTCGAG+TGG + chr4_1:63173566-63173585 Msa0562910:CDS 30.0%
!! AGATTTTAGCAACAAGGATT+TGG + chr4_1:63172464-63172483 Msa0562910:CDS 30.0%
! TGTACTGATACTAGATCATA+TGG + chr4_1:63172729-63172748 Msa0562910:intron 30.0%
TGATACTAGATCATATGGTT+TGG + chr4_1:63172734-63172753 Msa0562910:intron 30.0%
! AAAACGGTCTTATGATGAAA+AGG + chr4_1:63172766-63172785 Msa0562910:CDS 30.0%
TAAATGTGAAGTAGTAGTGA+GGG - chr4_1:63172823-63172842 None:intergenic 30.0%
ATAAATGTGAAGTAGTAGTG+AGG - chr4_1:63172824-63172843 None:intergenic 30.0%
!!! GTTACTTATTTTTGGTCATG+AGG + chr4_1:63172904-63172923 Msa0562910:intron 30.0%
TCCCTCATATTAAGATGTTA+GGG - chr4_1:63172958-63172977 None:intergenic 30.0%
AAAATGTCCATAAAGAGATG+TGG - chr4_1:63172988-63173007 None:intergenic 30.0%
ATTTGTGACTATTGAGTTCA+TGG - chr4_1:63173319-63173338 None:intergenic 30.0%
TTGCATAGCACAAATCATTA+AGG - chr4_1:63173350-63173369 None:intergenic 30.0%
ATGCAATCCTTGCAATATTT+AGG + chr4_1:63173364-63173383 Msa0562910:CDS 30.0%
! TGAATTTTTGCCCTCTATTT+GGG - chr4_1:63173400-63173419 None:intergenic 30.0%
ACATGAATGCATACAACATT+AGG - chr4_1:63173450-63173469 None:intergenic 30.0%
!!! GAAGTATGATATTTTTCGAG+TGG + chr4_1:63173566-63173585 Msa0562910:CDS 30.0%
!! CTGCATAGATTTTAGCAACA+AGG + chr4_1:63172458-63172477 Msa0562910:CDS 35.0%
TGGTCAACAATTACTACAGT+TGG - chr4_1:63172513-63172532 None:intergenic 35.0%
!! TCTATACTCAAGAGTATCCA+TGG + chr4_1:63172594-63172613 Msa0562910:CDS 35.0%
!! CAAGAGTATCCATGGAAATA+TGG + chr4_1:63172602-63172621 Msa0562910:CDS 35.0%
AAATGTGAAGTAGTAGTGAG+GGG - chr4_1:63172822-63172841 None:intergenic 35.0%
AGCCCTAACATCTTAATATG+AGG + chr4_1:63172953-63172972 Msa0562910:intron 35.0%
GCCCTAACATCTTAATATGA+GGG + chr4_1:63172954-63172973 Msa0562910:intron 35.0%
GTCCCTCATATTAAGATGTT+AGG - chr4_1:63172959-63172978 None:intergenic 35.0%
CTTTGATCCACATCTCTTTA+TGG + chr4_1:63172978-63172997 Msa0562910:intron 35.0%
GTTGGCATGACTTAATACAT+GGG - chr4_1:63173011-63173030 None:intergenic 35.0%
GGAAAGTAGAAATGCAAAAC+AGG - chr4_1:63173099-63173118 None:intergenic 35.0%
GCTAATGCATGTAACAAGAA+TGG + chr4_1:63173200-63173219 Msa0562910:CDS 35.0%
TATTTCCTCAATGCATATGC+TGG + chr4_1:63173240-63173259 Msa0562910:CDS 35.0%
AACGTGACCTAAATATTGCA+AGG - chr4_1:63173374-63173393 None:intergenic 35.0%
! TCACGTTTTTCCCAAATAGA+GGG + chr4_1:63173387-63173406 Msa0562910:CDS 35.0%
! CTGAATTTTTGCCCTCTATT+TGG - chr4_1:63173401-63173420 None:intergenic 35.0%
CAAATAGAGGGCAAAAATTC+AGG + chr4_1:63173399-63173418 Msa0562910:CDS 35.0%
AAATTCAGGCCTTTGCTTAA+AGG + chr4_1:63173413-63173432 Msa0562910:CDS 35.0%
AATTCAGGCCTTTGCTTAAA+GGG + chr4_1:63173414-63173433 Msa0562910:CDS 35.0%
! ACTTTTGACCCTTTAAGCAA+AGG - chr4_1:63173425-63173444 None:intergenic 35.0%
ATGTTGTATGCATTCATGTG+TGG + chr4_1:63173451-63173470 Msa0562910:CDS 35.0%
!!! CACTACAGTTTTGTTACTCA+TGG + chr4_1:63173478-63173497 Msa0562910:CDS 35.0%
GGATGATTCCTTATCTTGAA+CGG + chr4_1:63173526-63173545 Msa0562910:CDS 35.0%
! TTTCGAGTGGTATATGTTAG+TGG + chr4_1:63173579-63173598 Msa0562910:CDS 35.0%
AATGAAGAAGAGAGTAGACA+TGG + chr4_1:63173633-63173652 Msa0562910:CDS 35.0%
!! CTGCATAGATTTTAGCAACA+AGG + chr4_1:63172458-63172477 Msa0562910:CDS 35.0%
TGGTCAACAATTACTACAGT+TGG - chr4_1:63172513-63172532 None:intergenic 35.0%
!! TCTATACTCAAGAGTATCCA+TGG + chr4_1:63172594-63172613 Msa0562910:CDS 35.0%
!! CAAGAGTATCCATGGAAATA+TGG + chr4_1:63172602-63172621 Msa0562910:CDS 35.0%
AAATGTGAAGTAGTAGTGAG+GGG - chr4_1:63172822-63172841 None:intergenic 35.0%
AGCCCTAACATCTTAATATG+AGG + chr4_1:63172953-63172972 Msa0562910:intron 35.0%
GCCCTAACATCTTAATATGA+GGG + chr4_1:63172954-63172973 Msa0562910:intron 35.0%
GTCCCTCATATTAAGATGTT+AGG - chr4_1:63172959-63172978 None:intergenic 35.0%
CTTTGATCCACATCTCTTTA+TGG + chr4_1:63172978-63172997 Msa0562910:intron 35.0%
GTTGGCATGACTTAATACAT+GGG - chr4_1:63173011-63173030 None:intergenic 35.0%
GGAAAGTAGAAATGCAAAAC+AGG - chr4_1:63173099-63173118 None:intergenic 35.0%
GCTAATGCATGTAACAAGAA+TGG + chr4_1:63173200-63173219 Msa0562910:CDS 35.0%
TATTTCCTCAATGCATATGC+TGG + chr4_1:63173240-63173259 Msa0562910:CDS 35.0%
AACGTGACCTAAATATTGCA+AGG - chr4_1:63173374-63173393 None:intergenic 35.0%
! TCACGTTTTTCCCAAATAGA+GGG + chr4_1:63173387-63173406 Msa0562910:CDS 35.0%
! CTGAATTTTTGCCCTCTATT+TGG - chr4_1:63173401-63173420 None:intergenic 35.0%
CAAATAGAGGGCAAAAATTC+AGG + chr4_1:63173399-63173418 Msa0562910:CDS 35.0%
AAATTCAGGCCTTTGCTTAA+AGG + chr4_1:63173413-63173432 Msa0562910:CDS 35.0%
AATTCAGGCCTTTGCTTAAA+GGG + chr4_1:63173414-63173433 Msa0562910:CDS 35.0%
! ACTTTTGACCCTTTAAGCAA+AGG - chr4_1:63173425-63173444 None:intergenic 35.0%
ATGTTGTATGCATTCATGTG+TGG + chr4_1:63173451-63173470 Msa0562910:CDS 35.0%
!!! CACTACAGTTTTGTTACTCA+TGG + chr4_1:63173478-63173497 Msa0562910:CDS 35.0%
GGATGATTCCTTATCTTGAA+CGG + chr4_1:63173526-63173545 Msa0562910:CDS 35.0%
! TTTCGAGTGGTATATGTTAG+TGG + chr4_1:63173579-63173598 Msa0562910:CDS 35.0%
AATGAAGAAGAGAGTAGACA+TGG + chr4_1:63173633-63173652 Msa0562910:CDS 35.0%
! AGATCATATGGTTTGGAGCA+TGG + chr4_1:63172741-63172760 Msa0562910:intron 40.0%
AGATGTTAGGGCTATATCGA+CGG - chr4_1:63172946-63172965 None:intergenic 40.0%
CGTTGGCATGACTTAATACA+TGG - chr4_1:63173012-63173031 None:intergenic 40.0%
!! AGCATTGATAGCTGTAAGAG+AGG - chr4_1:63173183-63173202 None:intergenic 40.0%
TGTAACAAGAATGGCTGTCT+TGG + chr4_1:63173209-63173228 Msa0562910:CDS 40.0%
ATTCTCCAGCATATGCATTG+AGG - chr4_1:63173248-63173267 None:intergenic 40.0%
TATGCTGGAGAATCAAGAGA+TGG + chr4_1:63173255-63173274 Msa0562910:CDS 40.0%
! GTCACGTTTTTCCCAAATAG+AGG + chr4_1:63173386-63173405 Msa0562910:CDS 40.0%
ATGGAGAACCGTTCAAGATA+AGG - chr4_1:63173537-63173556 None:intergenic 40.0%
! AGATCATATGGTTTGGAGCA+TGG + chr4_1:63172741-63172760 Msa0562910:intron 40.0%
AGATGTTAGGGCTATATCGA+CGG - chr4_1:63172946-63172965 None:intergenic 40.0%
CGTTGGCATGACTTAATACA+TGG - chr4_1:63173012-63173031 None:intergenic 40.0%
!! AGCATTGATAGCTGTAAGAG+AGG - chr4_1:63173183-63173202 None:intergenic 40.0%
TGTAACAAGAATGGCTGTCT+TGG + chr4_1:63173209-63173228 Msa0562910:CDS 40.0%
ATTCTCCAGCATATGCATTG+AGG - chr4_1:63173248-63173267 None:intergenic 40.0%
TATGCTGGAGAATCAAGAGA+TGG + chr4_1:63173255-63173274 Msa0562910:CDS 40.0%
! GTCACGTTTTTCCCAAATAG+AGG + chr4_1:63173386-63173405 Msa0562910:CDS 40.0%
ATGGAGAACCGTTCAAGATA+AGG - chr4_1:63173537-63173556 None:intergenic 40.0%
! GGTTTGGAGCATGGCAAAAA+CGG + chr4_1:63172750-63172769 Msa0562910:CDS 45.0%
AGTGAGGGGTCATACTTACA+AGG - chr4_1:63172808-63172827 None:intergenic 45.0%
GTGCTTCTACAACTGTGATG+AGG + chr4_1:63172878-63172897 Msa0562910:intron 45.0%
! GGTTTGGAGCATGGCAAAAA+CGG + chr4_1:63172750-63172769 Msa0562910:CDS 45.0%
AGTGAGGGGTCATACTTACA+AGG - chr4_1:63172808-63172827 None:intergenic 45.0%
GTGCTTCTACAACTGTGATG+AGG + chr4_1:63172878-63172897 Msa0562910:intron 45.0%
TCCTCACGTGCTGAGAATCT+TGG + chr4_1:63172558-63172577 Msa0562910:CDS 50.0%
ACCAAGATTCTCAGCACGTG+AGG - chr4_1:63172562-63172581 None:intergenic 50.0%
TCCTCACGTGCTGAGAATCT+TGG + chr4_1:63172558-63172577 Msa0562910:CDS 50.0%
ACCAAGATTCTCAGCACGTG+AGG - chr4_1:63172562-63172581 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 63172447 63173674 63172447 ID=Msa0562910;Name=Msa0562910
chr4_1 mRNA 63172447 63173674 63172447 ID=Msa0562910-mRNA-1;Parent=Msa0562910;Name=Msa0562910-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|0|0|1|1|1|3|0|241
chr4_1 exon 63172447 63172623 63172447 ID=Msa0562910-mRNA-1:exon:19984;Parent=Msa0562910-mRNA-1
chr4_1 exon 63172743 63172808 63172743 ID=Msa0562910-mRNA-1:exon:19985;Parent=Msa0562910-mRNA-1
chr4_1 exon 63173192 63173674 63173192 ID=Msa0562910-mRNA-1:exon:19986;Parent=Msa0562910-mRNA-1
chr4_1 CDS 63172447 63172623 63172447 ID=Msa0562910-mRNA-1:cds;Parent=Msa0562910-mRNA-1
chr4_1 CDS 63172743 63172808 63172743 ID=Msa0562910-mRNA-1:cds;Parent=Msa0562910-mRNA-1
chr4_1 CDS 63173192 63173674 63173192 ID=Msa0562910-mRNA-1:cds;Parent=Msa0562910-mRNA-1
Gene Sequence

>Msa0562910

ATGGTTACATGCTGCATAGATTTTAGCAACAAGGATTTGGAAATAAGCTTCTTTGTTTTCAAGCCAACTGTAGTAATTGTTGACCATCTTATTCATGAAATCAAACAATTTTCCTCACGTGCTGAGAATCTTGGTTGTGTACAAAGCTCTATACTCAAGAGTATCCATGGAAATATGATCATATGGTTTGGAGCATGGCAAAAACGGTCTTATGATGAAAAGGATAAGTTGACAGCTGACCTTCTATCAATGCTAATGCATGTAACAAGAATGGCTGTCTTGGTTGAACACTATTTCCTCAATGCATATGCTGGAGAATCAAGAGATGGTTCTTCAACTATAAATTTTAGTACAGGTGATATAATCTCCATGAACTCAATAGTCACAAATATCAAAGACCTTAATGATTTGTGCTATGCAATCCTTGCAATATTTAGGTCACGTTTTTCCCAAATAGAGGGCAAAAATTCAGGCCTTTGCTTAAAGGGTCAAAAGTTACCTAATGTTGTATGCATTCATGTGTGGAAGTCACTACAGTTTTGTTACTCATGGATTCTAAATCCAGATTATAGAAATTGGATGATTCCTTATCTTGAACGGTTCTCCATTAACATGATGAAGTATGATATTTTTCGAGTGGTATATGTTAGTGGTGACAATATAGTTAATCTTCCTTGTGATTATAATGAAGAAGAGAGTAGACATGGACATGTTATACACAGCTAA

Protein sequence

>Msa0562910

MVTCCIDFSNKDLEISFFVFKPTVVIVDHLIHEIKQFSSRAENLGCVQSSILKSIHGNMIIWFGAWQKRSYDEKDKLTADLLSMLMHVTRMAVLVEHYFLNAYAGESRDGSSTINFSTGDIISMNSIVTNIKDLNDLCYAILAIFRSRFSQIEGKNSGLCLKGQKLPNVVCIHVWKSLQFCYSWILNPDYRNWMIPYLERFSINMMKYDIFRVVYVSGDNIVNLPCDYNEEESRHGHVIHS*