AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0573120

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0573120 MtrunA17_Chr8g0388921 100.000 160 0 0 1 160 320 479 3.26e-117 339
Msa0573120 MtrunA17_Chr8g0380471 61.635 159 61 0 1 159 319 477 1.68e-70 220
Msa0573120 MtrunA17_Chr8g0380931 56.209 153 65 2 6 156 351 503 3.77e-57 186
Msa0573120 MtrunA17_Chr5g0443401 50.955 157 76 1 1 157 321 476 7.59e-53 174
Msa0573120 MtrunA17_Chr5g0443731 49.686 159 78 2 2 159 315 472 2.08e-50 167
Msa0573120 MtrunA17_Chr3g0107511 44.720 161 86 2 1 158 317 477 3.44e-46 156
Msa0573120 MtrunA17_Chr3g0107501 43.478 161 88 2 1 158 317 477 1.44e-45 155
Msa0573120 MtrunA17_Chr4g0044851 40.764 157 91 2 1 156 313 468 1.30e-36 131
Msa0573120 MtrunA17_Chr8g0369791 44.898 147 80 1 13 159 321 466 3.52e-36 130
Msa0573120 MtrunA17_Chr5g0408181 43.537 147 82 1 13 159 319 464 6.45e-36 129
Msa0573120 MtrunA17_Chr1g0153371 44.366 142 78 1 13 154 325 465 2.36e-34 125
Msa0573120 MtrunA17_Chr4g0044841 37.342 158 96 3 1 156 314 470 2.99e-33 122
Msa0573120 MtrunA17_Chr5g0404861 37.037 162 93 4 1 157 1 158 4.29e-30 107
Msa0573120 MtrunA17_Chr5g0403931 37.654 162 96 3 2 158 319 480 3.77e-29 111
Msa0573120 MtrunA17_Chr7g0226771 37.956 137 85 0 3 139 316 452 1.03e-28 109
Msa0573120 MtrunA17_Chr1g0164371 41.259 143 83 1 13 155 332 473 4.00e-28 108
Msa0573120 MtrunA17_Chr8g0381541 37.500 136 84 1 2 136 332 467 6.61e-28 107
Msa0573120 MtrunA17_Chr5g0400421 32.903 155 103 1 2 155 333 487 1.20e-27 107
Msa0573120 MtrunA17_Chr5g0400411 34.194 155 101 1 2 155 332 486 3.14e-27 105
Msa0573120 MtrunA17_Chr8g0339861 35.333 150 95 2 2 150 330 478 8.23e-27 104
Msa0573120 MtrunA17_Chr8g0372411 36.129 155 95 2 1 153 321 473 1.89e-26 103
Msa0573120 MtrunA17_Chr8g0381571 37.209 129 80 1 2 129 332 460 2.22e-25 100
Msa0573120 MtrunA17_Chr2g0293411 34.810 158 88 2 2 158 330 473 2.39e-25 100
Msa0573120 MtrunA17_Chr8g0341951 35.537 121 78 0 10 130 326 446 2.42e-25 100
Msa0573120 MtrunA17_Chr8g0372711 37.692 130 80 1 1 130 328 456 4.83e-25 99.8
Msa0573120 MtrunA17_Chr3g0113321 36.306 157 89 4 4 153 311 463 7.27e-25 99.4
Msa0573120 MtrunA17_Chr4g0000151 38.739 111 68 0 40 150 24 134 2.42e-24 92.0
Msa0573120 MtrunA17_Chr2g0293421 37.037 135 83 2 2 135 332 465 2.57e-24 97.8
Msa0573120 MtrunA17_Chr3g0089351 34.532 139 88 2 17 154 255 391 2.97e-24 97.1
Msa0573120 MtrunA17_Chr6g0455461 35.507 138 88 1 1 137 307 444 3.49e-24 97.1
Msa0573120 MtrunA17_Chr6g0463541 40.777 103 60 1 51 153 4 105 6.20e-24 90.1
Msa0573120 MtrunA17_Chr8g0381561 35.455 110 71 0 20 129 2 111 1.66e-23 89.4
Msa0573120 MtrunA17_Chr7g0219961 34.722 144 92 2 13 155 318 460 2.94e-23 94.7
Msa0573120 MtrunA17_Chr3g0078411 35.075 134 86 1 1 133 307 440 3.82e-23 94.4
Msa0573120 MtrunA17_Chr3g0141801 36.691 139 87 1 1 139 327 464 8.22e-23 93.6
Msa0573120 MtrunA17_Chr5g0437001 40.367 109 60 2 21 125 1 108 1.23e-22 88.2
Msa0573120 MtrunA17_Chr5g0397971 34.146 123 79 1 13 133 324 446 1.82e-22 92.4
Msa0573120 MtrunA17_Chr3g0089361 32.192 146 96 2 10 154 330 473 3.84e-21 88.6
Msa0573120 MtrunA17_Chr6g0478161 32.374 139 87 1 5 136 331 469 2.89e-18 80.5
Msa0573120 MtrunA17_Chr0c19g0493771 32.000 125 79 1 16 134 498 622 2.64e-17 77.8
Msa0573120 MtrunA17_Chr4g0035921 29.677 155 101 2 2 148 346 500 3.11e-16 74.7
Msa0573120 MtrunA17_Chr3g0128461 34.211 152 89 5 2 143 363 513 9.46e-15 70.5
Msa0573120 MtrunA17_Chr5g0431501 33.333 135 85 2 2 131 335 469 1.03e-14 70.5
Msa0573120 MtrunA17_Chr5g0431461 33.333 135 85 2 2 131 335 469 1.03e-14 70.5
Msa0573120 MtrunA17_Chr5g0400391 36.145 83 53 0 74 156 4 86 1.78e-14 65.5
Msa0573120 MtrunA17_Chr4g0011871 30.714 140 90 3 2 136 330 467 1.94e-14 69.7
Msa0573120 MtrunA17_Chr4g0058281 33.058 121 76 2 16 131 360 480 2.77e-14 69.3
Msa0573120 MtrunA17_Chr4g0058231 34.677 124 70 3 16 131 348 468 4.94e-14 68.6
Msa0573120 MtrunA17_Chr5g0446161 32.773 119 76 1 17 131 349 467 6.48e-14 68.2
Msa0573120 MtrunA17_Chr1g0161661 36.250 80 51 0 76 155 18 97 7.32e-14 63.9
Msa0573120 MtrunA17_Chr5g0405001 27.742 155 104 2 2 148 346 500 1.28e-13 67.4
Msa0573120 MtrunA17_Chr5g0431511 31.373 153 94 4 2 145 335 485 1.31e-13 67.4
Msa0573120 MtrunA17_Chr2g0313821 30.081 123 81 2 14 131 366 488 1.85e-13 67.0
Msa0573120 MtrunA17_Chr2g0313791 32.787 122 77 3 15 131 362 483 1.93e-13 66.6
Msa0573120 MtrunA17_Chr4g0039391 34.507 142 88 3 2 139 336 476 1.95e-13 66.6
Msa0573120 MtrunA17_Chr1g0179811 29.927 137 88 3 10 139 383 518 2.00e-13 66.6
Msa0573120 MtrunA17_Chr7g0218601 31.200 125 82 1 11 131 342 466 2.12e-13 66.6
Msa0573120 MtrunA17_Chr4g0058211 30.370 135 89 2 2 131 51 185 2.15e-13 65.1
Msa0573120 MtrunA17_Chr4g0011851 30.986 142 87 4 2 136 48 185 2.34e-13 65.1
Msa0573120 MtrunA17_Chr8g0373431 46.667 60 31 1 96 155 5 63 7.13e-13 60.5
Msa0573120 MtrunA17_Chr8g0364651 30.303 132 84 2 16 139 344 475 8.78e-13 64.7
Msa0573120 MtrunA17_Chr2g0313781 31.707 123 79 3 14 131 378 500 9.90e-13 64.7
Msa0573120 MtrunA17_Chr4g0058191 30.370 135 89 2 2 131 323 457 1.00e-12 64.7
Msa0573120 MtrunA17_Chr8g0334941 29.412 136 86 2 10 135 169 304 1.04e-12 64.3
Msa0573120 MtrunA17_Chr2g0307301 30.081 123 81 2 14 131 368 490 1.15e-12 64.7
Msa0573120 MtrunA17_Chr8g0334891 29.412 136 86 2 10 135 324 459 1.22e-12 64.3
Msa0573120 MtrunA17_Chr3g0116741 31.061 132 83 4 17 142 363 492 1.77e-12 63.9
Msa0573120 MtrunA17_Chr7g0218611 28.800 125 85 1 11 131 343 467 1.81e-12 63.9
Msa0573120 MtrunA17_Chr5g0413911 32.558 129 80 2 16 137 376 504 2.20e-12 63.9
Msa0573120 MtrunA17_Chr2g0313801 31.707 123 79 3 14 131 366 488 3.67e-12 63.2
Msa0573120 MtrunA17_Chr6g0457421 30.172 116 80 1 16 130 363 478 3.84e-12 63.2
Msa0573120 MtrunA17_Chr2g0314781 27.044 159 106 3 2 155 17 170 4.55e-12 60.8
Msa0573120 MtrunA17_Chr2g0313811 30.894 123 80 3 14 131 378 500 4.60e-12 62.8
Msa0573120 MtrunA17_Chr5g0419691 30.000 140 91 3 2 136 329 466 4.95e-12 62.8
Msa0573120 MtrunA17_Chr1g0160851 27.545 167 112 4 2 159 330 496 5.19e-12 62.8
Msa0573120 MtrunA17_Chr4g0053621 28.058 139 94 3 2 137 335 470 5.53e-12 62.8
Msa0573120 MtrunA17_Chr8g0373441 38.202 89 53 2 2 88 316 404 6.11e-12 62.4
Msa0573120 MtrunA17_Chr3g0135841 34.483 116 67 3 20 128 390 503 8.31e-12 62.0
Msa0573120 MtrunA17_Chr1g0161641 31.579 76 52 0 54 129 13 88 8.34e-12 58.2
Msa0573120 MtrunA17_Chr5g0446151 31.933 119 77 1 17 131 350 468 9.08e-12 62.0
Msa0573120 MtrunA17_Chr3g0101601 34.127 126 73 3 14 131 341 464 1.01e-11 62.0
Msa0573120 MtrunA17_Chr5g0403351 28.148 135 92 2 2 131 336 470 1.18e-11 61.6
Msa0573120 MtrunA17_Chr2g0280121 31.707 123 77 2 16 131 357 479 1.19e-11 61.6
Msa0573120 MtrunA17_Chr4g0028321 31.068 103 64 2 34 131 24 124 1.23e-11 59.3
Msa0573120 MtrunA17_Chr1g0160861 27.545 167 112 4 2 159 171 337 1.25e-11 61.2
Msa0573120 MtrunA17_Chr8g0382901 34.711 121 72 3 17 131 323 442 1.26e-11 61.6
Msa0573120 MtrunA17_Chr7g0265611 31.148 122 77 3 17 131 363 484 1.42e-11 61.2
Msa0573120 MtrunA17_Chr3g0116731 32.075 106 71 1 21 125 1 106 1.58e-11 58.5
Msa0573120 MtrunA17_Chr1g0205361 27.273 165 111 4 2 157 330 494 1.61e-11 61.2
Msa0573120 MtrunA17_Chr3g0117301 32.479 117 72 3 21 131 1 116 1.96e-11 57.8
Msa0573120 MtrunA17_Chr2g0314731 28.947 152 96 4 10 155 338 483 2.00e-11 60.8
Msa0573120 MtrunA17_Chr7g0215181 29.508 122 85 1 10 130 359 480 2.18e-11 60.8
Msa0573120 MtrunA17_Chr1g0198191 29.008 131 85 2 17 139 362 492 2.41e-11 60.8
Msa0573120 MtrunA17_Chr8g0354531 30.081 123 81 3 14 131 495 617 2.67e-11 60.8
Msa0573120 MtrunA17_Chr3g0101621 32.540 126 75 3 14 131 335 458 4.16e-11 60.1
Msa0573120 MtrunA17_Chr2g0280081 30.081 123 79 2 16 131 852 974 4.53e-11 60.1
Msa0573120 MtrunA17_Chr2g0280081 30.328 122 78 2 17 131 352 473 5.49e-11 59.7
Msa0573120 MtrunA17_Chr4g0046331 26.573 143 92 2 2 131 333 475 5.36e-11 59.7
Msa0573120 MtrunA17_Chr1g0151221 29.371 143 95 2 2 138 363 505 7.81e-11 59.3
Msa0573120 MtrunA17_Chr8g0344181 34.167 120 72 3 17 130 354 472 9.87e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0573120 AT5G36110.1 73.469 147 38 1 13 158 330 476 9.89e-78 239
Msa0573120 AT5G36130.1 74.820 139 34 1 21 158 1 139 1.65e-75 222
Msa0573120 AT2G42850.1 51.282 156 75 1 2 157 328 482 8.22e-52 172
Msa0573120 AT2G29090.3 44.444 153 84 1 5 157 235 386 8.38e-42 143
Msa0573120 AT2G29090.2 44.444 153 84 1 5 157 330 481 1.12e-40 142
Msa0573120 AT2G29090.1 44.444 153 84 1 5 157 330 481 1.12e-40 142
Msa0573120 AT5G45340.1 45.161 155 84 1 1 155 306 459 2.72e-39 138
Msa0573120 AT4G19230.1 42.581 155 88 1 1 155 306 459 5.62e-36 129
Msa0573120 AT3G19270.2 42.466 146 83 1 13 158 322 466 1.48e-34 125
Msa0573120 AT5G45340.2 44.118 136 75 1 1 136 306 440 1.93e-32 120
Msa0573120 AT4G19230.2 41.791 134 77 1 1 134 306 438 1.58e-30 115
Msa0573120 AT3G19270.1 43.220 118 66 1 13 130 322 438 8.18e-29 110
Msa0573120 AT4G36380.1 35.220 159 97 3 1 155 353 509 3.27e-28 109
Msa0573120 AT5G05690.3 39.456 147 79 4 13 153 202 344 2.63e-27 104
Msa0573120 AT2G32440.2 34.641 153 100 0 3 155 332 484 6.50e-27 105
Msa0573120 AT2G32440.1 34.641 153 100 0 3 155 332 484 6.50e-27 105
Msa0573120 AT5G05690.1 39.456 147 79 4 13 153 321 463 1.14e-26 104
Msa0573120 AT3G50660.1 36.095 169 93 4 1 155 342 509 2.39e-26 103
Msa0573120 AT1G05160.2 35.484 155 98 2 3 155 333 487 1.63e-25 101
Msa0573120 AT1G05160.1 35.484 155 98 2 3 155 333 487 1.63e-25 101
Msa0573120 AT3G13730.1 37.956 137 81 2 9 145 344 476 2.02e-25 101
Msa0573120 AT5G38970.2 33.548 155 101 2 5 158 231 384 1.88e-23 95.1
Msa0573120 AT5G38970.4 33.548 155 101 2 5 158 231 384 1.88e-23 95.1
Msa0573120 AT3G13730.2 38.017 121 75 0 9 129 344 464 2.38e-23 95.1
Msa0573120 AT5G38970.1 33.548 155 101 2 5 158 312 465 3.67e-23 94.7
Msa0573120 AT1G12740.1 34.146 123 79 1 13 133 324 446 2.47e-22 92.4
Msa0573120 AT1G12740.2 34.146 123 79 1 13 133 330 452 2.55e-22 92.4
Msa0573120 AT3G30180.1 33.333 141 93 1 1 140 308 448 6.15e-22 91.3
Msa0573120 AT1G78490.1 33.117 154 97 4 5 155 323 473 2.45e-21 89.7
Msa0573120 AT1G12740.4 33.333 129 78 2 13 133 324 452 3.03e-20 86.7
Msa0573120 AT5G36110.2 65.455 55 19 0 13 67 330 384 1.82e-19 84.0
Msa0573120 AT5G48000.2 31.544 149 98 3 10 155 326 473 3.31e-19 83.6
Msa0573120 AT5G48000.4 31.544 149 98 3 10 155 326 473 3.31e-19 83.6
Msa0573120 AT5G48000.7 31.544 149 98 3 10 155 326 473 3.31e-19 83.6
Msa0573120 AT5G48000.6 31.544 149 98 3 10 155 326 473 3.31e-19 83.6
Msa0573120 AT5G48000.5 31.544 149 98 3 10 155 326 473 3.31e-19 83.6
Msa0573120 AT5G48000.3 31.544 149 98 3 10 155 326 473 3.31e-19 83.6
Msa0573120 AT5G48000.1 31.544 149 98 3 10 155 367 514 4.48e-19 83.2
Msa0573120 AT5G14400.1 30.573 157 90 2 3 159 226 363 8.14e-19 82.0
Msa0573120 AT5G14400.2 30.573 157 90 2 3 159 280 417 8.52e-19 82.4
Msa0573120 AT1G55940.2 32.192 146 93 4 13 155 345 487 1.89e-18 81.6
Msa0573120 AT1G55940.1 33.613 119 76 2 13 128 345 463 3.18e-18 80.9
Msa0573120 AT4G15396.1 32.231 121 77 3 13 130 326 444 1.21e-17 79.3
Msa0573120 AT1G19630.1 31.884 138 93 1 2 139 319 455 1.73e-17 79.0
Msa0573120 AT4G15300.2 33.835 133 82 4 2 130 313 443 2.41e-17 78.2
Msa0573120 AT4G15300.1 33.835 133 82 4 2 130 325 455 2.44e-17 78.2
Msa0573120 AT4G15300.3 33.835 133 82 4 2 130 303 433 2.69e-17 78.2
Msa0573120 AT3G44970.1 33.083 133 84 3 1 130 319 449 7.30e-17 77.0
Msa0573120 AT2G42850.2 54.098 61 28 0 2 62 328 388 1.31e-16 75.9
Msa0573120 AT4G15396.3 31.624 117 76 2 13 126 326 441 1.40e-16 76.3
Msa0573120 AT1G73340.1 31.852 135 85 1 9 136 325 459 3.20e-16 75.1
Msa0573120 AT5G14400.3 31.250 128 70 1 3 130 280 389 4.70e-16 74.3
Msa0573120 AT4G15393.2 30.579 121 79 3 13 130 324 442 1.02e-15 73.6
Msa0573120 AT3G30290.1 32.558 129 80 4 5 130 249 373 4.04e-15 72.0
Msa0573120 AT1G31800.1 32.231 121 76 1 17 131 420 540 5.17e-15 71.6
Msa0573120 AT1G19630.3 29.545 132 92 1 2 133 319 449 5.20e-15 71.6
Msa0573120 AT1G65670.1 30.147 136 89 4 3 134 310 443 9.73e-15 70.9
Msa0573120 AT3G53130.1 30.303 132 84 3 15 139 388 518 3.48e-14 69.3
Msa0573120 AT3G28740.1 31.707 123 82 2 12 133 347 468 6.11e-14 68.6
Msa0573120 AT5G35715.1 29.814 161 100 4 8 157 275 433 6.80e-14 68.6
Msa0573120 AT3G48310.1 34.586 133 73 5 16 139 335 462 1.02e-13 68.2
Msa0573120 AT5G57220.1 33.065 124 79 3 12 133 334 455 1.74e-13 67.4
Msa0573120 AT2G26170.1 36.641 131 70 5 16 135 371 499 3.19e-13 66.6
Msa0573120 AT2G26170.2 36.641 131 70 5 16 135 288 416 3.24e-13 66.6
Msa0573120 AT4G37410.1 27.737 137 95 2 1 133 325 461 3.25e-13 66.6
Msa0573120 AT4G37360.1 29.605 152 102 3 2 152 329 476 3.47e-13 66.6
Msa0573120 AT5G24900.1 33.065 124 73 4 30 143 393 516 5.79e-13 65.9
Msa0573120 AT3G25180.1 29.197 137 92 2 2 133 342 478 6.92e-13 65.5
Msa0573120 AT4G37330.1 31.884 138 90 3 1 136 325 460 6.98e-13 65.5
Msa0573120 AT4G37430.1 29.286 140 95 2 2 137 329 468 9.53e-13 65.1
Msa0573120 AT5G24910.1 33.333 120 75 3 16 130 384 503 1.41e-12 64.7
Msa0573120 AT5G67310.1 32.168 143 90 4 2 142 340 477 2.78e-12 63.9
Msa0573120 AT4G37320.1 28.571 133 93 2 2 133 329 460 2.82e-12 63.9
Msa0573120 AT4G13310.1 28.571 147 93 4 2 139 329 472 3.20e-12 63.5
Msa0573120 AT1G11680.1 28.676 136 87 2 10 135 326 461 4.41e-12 63.2
Msa0573120 AT1G64930.1 28.800 125 81 2 17 133 356 480 5.30e-12 63.2
Msa0573120 AT2G28860.1 30.882 136 87 3 6 136 331 464 5.41e-12 63.2
Msa0573120 AT4G13290.1 29.333 150 88 5 2 139 322 465 5.56e-12 63.2
Msa0573120 AT4G37370.1 29.323 133 92 2 2 133 328 459 7.09e-12 62.8
Msa0573120 AT5G24900.2 32.331 133 80 4 21 143 267 399 8.98e-12 62.4
Msa0573120 AT4G37400.1 29.286 140 91 3 1 133 323 461 1.34e-11 62.0
Msa0573120 AT3G26330.1 27.397 146 99 3 2 142 330 473 1.64e-11 61.6
Msa0573120 AT4G37340.1 30.435 138 92 3 2 137 329 464 2.23e-11 61.2
Msa0573120 AT3G26230.1 29.375 160 94 6 11 157 341 494 2.78e-11 60.8
Msa0573120 AT1G64940.1 28.455 123 82 2 17 133 358 480 5.31e-11 60.1
Msa0573120 AT5G04630.1 26.027 146 100 2 2 139 340 485 5.95e-11 60.1
Msa0573120 AT3G03470.1 29.771 131 82 2 17 137 354 484 6.02e-11 60.1
Msa0573120 AT2G12190.1 27.941 136 90 2 17 144 357 492 6.02e-11 60.1
Msa0573120 AT5G52400.1 34.188 117 69 4 18 129 376 489 7.25e-11 59.7

Find 51 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTATATGTGTAGCTTTAT+TGG 0.183186 4_1:+76181226 Msa0573120:intron
CTCTAAACATGCACAAAATT+AGG 0.198757 4_1:-76180910 None:intergenic
CTCCACTCCAAGGAGGTTTC+AGG 0.207186 4_1:+76181042 Msa0573120:CDS
CCATTTCCTTCAAATCTTGT+TGG 0.308493 4_1:-76181306 None:intergenic
TTTAAGCTTTGTGTGGATAA+AGG 0.325283 4_1:-76181518 None:intergenic
CATTTCCTTCAAATCTTGTT+GGG 0.327422 4_1:-76181305 None:intergenic
CAGCAGGAGAATTGTTGAAT+TGG 0.334479 4_1:+76180958 Msa0573120:CDS
CTTGTTGGGTCAAATTTCTC+TGG 0.350406 4_1:-76181291 None:intergenic
CCAAATGGCACAAAAGTATA+AGG 0.354997 4_1:-76181336 None:intergenic
ATCACTGACTTTATGTTCAA+TGG 0.364510 4_1:+76181071 Msa0573120:CDS
GGGAGATCCTTTGCAGGGAT+GGG 0.372416 4_1:-76181489 None:intergenic
TTGTGCATGTTTAGAGCAAA+TGG 0.375262 4_1:+76180917 Msa0573120:exon
TTTGACCCAACAAGATTTGA+AGG 0.391588 4_1:+76181300 Msa0573120:CDS
CCAACAAGATTTGAAGGAAA+TGG 0.393625 4_1:+76181306 Msa0573120:CDS
CGAATTGGGAGATCCTTTGC+AGG 0.407892 4_1:-76181495 None:intergenic
TTGTGTGGATAAAGGCGAAT+TGG 0.408235 4_1:-76181510 None:intergenic
CAATTCAACAATTCTCCTGC+TGG 0.410007 4_1:-76180957 None:intergenic
TCAACAATAATCTTCTCATC+TGG 0.415793 4_1:-76181459 None:intergenic
AGCAGGAGAATTGTTGAATT+GGG 0.421810 4_1:+76180959 Msa0573120:CDS
TGGGAGATCCTTTGCAGGGA+TGG 0.425546 4_1:-76181490 None:intergenic
TCATGCACAATTTAGTGAAA+AGG 0.434920 4_1:+76181415 Msa0573120:CDS
CAATGGCTTCTCAATTCCTA+AGG 0.438143 4_1:+76181088 Msa0573120:CDS
TGTGTGGATAAAGGCGAATT+GGG 0.442584 4_1:-76181509 None:intergenic
TCAAGAAAATGAAATACTCT+TGG 0.452559 4_1:+76180988 Msa0573120:CDS
TGGGTCAAATTTCTCTGGCA+TGG 0.457169 4_1:-76181286 None:intergenic
ATTTAGTGAAAAGGTTTAAG+TGG 0.469950 4_1:+76181424 Msa0573120:CDS
TTAATTACCTTCCATCCCTT+AGG 0.470507 4_1:-76181104 None:intergenic
TGGCTTCCCTGAAACCTCCT+TGG 0.470918 4_1:-76181049 None:intergenic
TTTAGTGAAAAGGTTTAAGT+GGG 0.478571 4_1:+76181425 Msa0573120:CDS
TCCTTTGCAGGGATGGGGAA+TGG 0.480520 4_1:-76181483 None:intergenic
GGAGATCCTTTGCAGGGATG+GGG 0.484284 4_1:-76181488 None:intergenic
TCCACTCCAAGGAGGTTTCA+GGG 0.488222 4_1:+76181043 Msa0573120:CDS
GGGTCAAATTTCTCTGGCAT+GGG 0.502359 4_1:-76181285 None:intergenic
ATCCTTGGTCCTCCACCAAA+TGG 0.503860 4_1:-76181351 None:intergenic
GAAAAGGTTTAAGTGGGAAA+AGG 0.508814 4_1:+76181431 Msa0573120:CDS
TGAAACCTCCTTGGAGTGGA+GGG 0.516382 4_1:-76181040 None:intergenic
CTCAATTCCTAAGGGATGGA+AGG 0.533431 4_1:+76181097 Msa0573120:CDS
CTGAAACCTCCTTGGAGTGG+AGG 0.543395 4_1:-76181041 None:intergenic
GCTTCTCAATTCCTAAGGGA+TGG 0.548330 4_1:+76181093 Msa0573120:CDS
TCCCTGAAACCTCCTTGGAG+TGG 0.550574 4_1:-76181044 None:intergenic
GGCACAAAAGTATAAGGAGC+TGG 0.558481 4_1:-76181330 None:intergenic
TCCATTCCCCATCCCTGCAA+AGG 0.577603 4_1:+76181482 Msa0573120:CDS
ATTGCAAAATCGAAACCAGC+AGG 0.580297 4_1:+76180942 Msa0573120:CDS
AGACTTTCCCCTCCACTCCA+AGG 0.589332 4_1:+76181032 Msa0573120:CDS
AATGGCTTCTCAATTCCTAA+GGG 0.600833 4_1:+76181089 Msa0573120:CDS
GGAGGACCAAGGATGTGCCC+TGG 0.603367 4_1:+76181360 Msa0573120:CDS
GAAACCTCCTTGGAGTGGAG+GGG 0.607503 4_1:-76181039 None:intergenic
ATTGAACATAAAGTCAGTGA+TGG 0.611283 4_1:-76181069 None:intergenic
CTTTCCCCTCCACTCCAAGG+AGG 0.633438 4_1:+76181035 Msa0573120:CDS
GAATTGGGAGATCCTTTGCA+GGG 0.633506 4_1:-76181494 None:intergenic
TGCCATTTGGTGGAGGACCA+AGG 0.636114 4_1:+76181349 Msa0573120:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTGGTTTGAATCATATA+AGG + chr4_1:76181157-76181176 Msa0573120:intron 20.0%
!!! ATTTATATGTGTAGCTTTAT+TGG + chr4_1:76181226-76181245 Msa0573120:intron 20.0%
!!! AAGAGTATTTCATTTTCTTG+AGG - chr4_1:76180989-76181008 None:intergenic 25.0%
! TCAAGAAAATGAAATACTCT+TGG + chr4_1:76180988-76181007 Msa0573120:CDS 25.0%
!!! CACATATATCTCTTTTTTGT+TGG + chr4_1:76181127-76181146 Msa0573120:intron 25.0%
!!! TTTTGTTGGTCTTTACTTTT+TGG + chr4_1:76181141-76181160 Msa0573120:intron 25.0%
! ATTTAGTGAAAAGGTTTAAG+TGG + chr4_1:76181424-76181443 Msa0573120:CDS 25.0%
! TTTAGTGAAAAGGTTTAAGT+GGG + chr4_1:76181425-76181444 Msa0573120:CDS 25.0%
CTCTAAACATGCACAAAATT+AGG - chr4_1:76180913-76180932 None:intergenic 30.0%
ATTGAACATAAAGTCAGTGA+TGG - chr4_1:76181072-76181091 None:intergenic 30.0%
!! ATCACTGACTTTATGTTCAA+TGG + chr4_1:76181071-76181090 Msa0573120:CDS 30.0%
CAAATTAGTTCCCACAAAAA+TGG - chr4_1:76181194-76181213 None:intergenic 30.0%
CATTTCCTTCAAATCTTGTT+GGG - chr4_1:76181308-76181327 None:intergenic 30.0%
TCATGCACAATTTAGTGAAA+AGG + chr4_1:76181415-76181434 Msa0573120:CDS 30.0%
TCAACAATAATCTTCTCATC+TGG - chr4_1:76181462-76181481 None:intergenic 30.0%
! TTGTGCATGTTTAGAGCAAA+TGG + chr4_1:76180917-76180936 Msa0573120:exon 35.0%
AGCAGGAGAATTGTTGAATT+GGG + chr4_1:76180959-76180978 Msa0573120:CDS 35.0%
AATGGCTTCTCAATTCCTAA+GGG + chr4_1:76181089-76181108 Msa0573120:CDS 35.0%
TTAATTACCTTCCATCCCTT+AGG - chr4_1:76181107-76181126 None:intergenic 35.0%
!! TATGAGTGCTACCATTTTTG+TGG + chr4_1:76181180-76181199 Msa0573120:intron 35.0%
!! ATGAGTGCTACCATTTTTGT+GGG + chr4_1:76181181-76181200 Msa0573120:intron 35.0%
TTTGACCCAACAAGATTTGA+AGG + chr4_1:76181300-76181319 Msa0573120:CDS 35.0%
CCATTTCCTTCAAATCTTGT+TGG - chr4_1:76181309-76181328 None:intergenic 35.0%
CCAACAAGATTTGAAGGAAA+TGG + chr4_1:76181306-76181325 Msa0573120:CDS 35.0%
! CCAAATGGCACAAAAGTATA+AGG - chr4_1:76181339-76181358 None:intergenic 35.0%
!! CCTTATACTTTTGTGCCATT+TGG + chr4_1:76181336-76181355 Msa0573120:CDS 35.0%
!!! TATACTTTTGTGCCATTTGG+TGG + chr4_1:76181339-76181358 Msa0573120:CDS 35.0%
! ATCTTGCATACTCTTTTCCA+GGG - chr4_1:76181380-76181399 None:intergenic 35.0%
! AATCTTGCATACTCTTTTCC+AGG - chr4_1:76181381-76181400 None:intergenic 35.0%
GAAAAGGTTTAAGTGGGAAA+AGG + chr4_1:76181431-76181450 Msa0573120:CDS 35.0%
ATTGCAAAATCGAAACCAGC+AGG + chr4_1:76180942-76180961 Msa0573120:CDS 40.0%
CAATTCAACAATTCTCCTGC+TGG - chr4_1:76180960-76180979 None:intergenic 40.0%
CAGCAGGAGAATTGTTGAAT+TGG + chr4_1:76180958-76180977 Msa0573120:CDS 40.0%
CAATGGCTTCTCAATTCCTA+AGG + chr4_1:76181088-76181107 Msa0573120:CDS 40.0%
CTTGTTGGGTCAAATTTCTC+TGG - chr4_1:76181294-76181313 None:intergenic 40.0%
TGTGTGGATAAAGGCGAATT+GGG - chr4_1:76181512-76181531 None:intergenic 40.0%
TTGTGTGGATAAAGGCGAAT+TGG - chr4_1:76181513-76181532 None:intergenic 40.0%
GCTTCTCAATTCCTAAGGGA+TGG + chr4_1:76181093-76181112 Msa0573120:CDS 45.0%
CTCAATTCCTAAGGGATGGA+AGG + chr4_1:76181097-76181116 Msa0573120:CDS 45.0%
GGGTCAAATTTCTCTGGCAT+GGG - chr4_1:76181288-76181307 None:intergenic 45.0%
TGGGTCAAATTTCTCTGGCA+TGG - chr4_1:76181289-76181308 None:intergenic 45.0%
! GGCACAAAAGTATAAGGAGC+TGG - chr4_1:76181333-76181352 None:intergenic 45.0%
!!! ACTTTTGTGCCATTTGGTGG+AGG + chr4_1:76181342-76181361 Msa0573120:CDS 45.0%
GAATTGGGAGATCCTTTGCA+GGG - chr4_1:76181497-76181516 None:intergenic 45.0%
TGAAACCTCCTTGGAGTGGA+GGG - chr4_1:76181043-76181062 None:intergenic 50.0%
TCCACTCCAAGGAGGTTTCA+GGG + chr4_1:76181043-76181062 Msa0573120:CDS 50.0%
ATCCTTGGTCCTCCACCAAA+TGG - chr4_1:76181354-76181373 None:intergenic 50.0%
! TCTTTTCCAGGGCACATCCT+TGG - chr4_1:76181369-76181388 None:intergenic 50.0%
CGAATTGGGAGATCCTTTGC+AGG - chr4_1:76181498-76181517 None:intergenic 50.0%
AGACTTTCCCCTCCACTCCA+AGG + chr4_1:76181032-76181051 Msa0573120:CDS 55.0%
GAAACCTCCTTGGAGTGGAG+GGG - chr4_1:76181042-76181061 None:intergenic 55.0%
CTGAAACCTCCTTGGAGTGG+AGG - chr4_1:76181044-76181063 None:intergenic 55.0%
CTCCACTCCAAGGAGGTTTC+AGG + chr4_1:76181042-76181061 Msa0573120:CDS 55.0%
TCCCTGAAACCTCCTTGGAG+TGG - chr4_1:76181047-76181066 None:intergenic 55.0%
TGGCTTCCCTGAAACCTCCT+TGG - chr4_1:76181052-76181071 None:intergenic 55.0%
! TGCCATTTGGTGGAGGACCA+AGG + chr4_1:76181349-76181368 Msa0573120:CDS 55.0%
TCCATTCCCCATCCCTGCAA+AGG + chr4_1:76181482-76181501 Msa0573120:CDS 55.0%
TCCTTTGCAGGGATGGGGAA+TGG - chr4_1:76181486-76181505 None:intergenic 55.0%
GGAGATCCTTTGCAGGGATG+GGG - chr4_1:76181491-76181510 None:intergenic 55.0%
GGGAGATCCTTTGCAGGGAT+GGG - chr4_1:76181492-76181511 None:intergenic 55.0%
TGGGAGATCCTTTGCAGGGA+TGG - chr4_1:76181493-76181512 None:intergenic 55.0%
CTTTCCCCTCCACTCCAAGG+AGG + chr4_1:76181035-76181054 Msa0573120:CDS 60.0%
GGAGGACCAAGGATGTGCCC+TGG + chr4_1:76181360-76181379 Msa0573120:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 76180886 76181536 76180886 ID=Msa0573120;Name=Msa0573120
chr4_1 mRNA 76180886 76181536 76180886 ID=Msa0573120-mRNA-1;Parent=Msa0573120;Name=Msa0573120-mRNA-1;_AED=0.14;_eAED=0.14;_QI=50|1|0.5|1|1|0.5|2|0|160
chr4_1 exon 76180886 76181118 76180886 ID=Msa0573120-mRNA-1:exon:26486;Parent=Msa0573120-mRNA-1
chr4_1 exon 76181240 76181536 76181240 ID=Msa0573120-mRNA-1:exon:26487;Parent=Msa0573120-mRNA-1
chr4_1 five_prime_UTR 76180886 76180935 76180886 ID=Msa0573120-mRNA-1:five_prime_utr;Parent=Msa0573120-mRNA-1
chr4_1 CDS 76180936 76181118 76180936 ID=Msa0573120-mRNA-1:cds;Parent=Msa0573120-mRNA-1
chr4_1 CDS 76181240 76181536 76181240 ID=Msa0573120-mRNA-1:cds;Parent=Msa0573120-mRNA-1
Gene Sequence

>Msa0573120

ATGGAAATTGCAAAATCGAAACCAGCAGGAGAATTGTTGAATTGGGATGACCTCAAGAAAATGAAATACTCTTGGAACGTAGCTTGTGAAGTAATGAGACTTTCCCCTCCACTCCAAGGAGGTTTCAGGGAAGCCATCACTGACTTTATGTTCAATGGCTTCTCAATTCCTAAGGGATGGAAGCTTTATTGGAGTGCAAATTCAACACATAAGAACGCAGAATGTTTTCCCATGCCAGAGAAATTTGACCCAACAAGATTTGAAGGAAATGGACCAGCTCCTTATACTTTTGTGCCATTTGGTGGAGGACCAAGGATGTGCCCTGGAAAAGAGTATGCAAGATTAGAAATACTAGTTTTCATGCACAATTTAGTGAAAAGGTTTAAGTGGGAAAAGGTGATTCCAGATGAGAAGATTATTGTTGATCCATTCCCCATCCCTGCAAAGGATCTCCCAATTCGCCTTTATCCACACAAAGCT
Protein sequence

>Msa0573120

MEIAKSKPAGELLNWDDLKKMKYSWNVACEVMRLSPPLQGGFREAITDFMFNGFSIPKGWKLYWSANSTHKNAECFPMPEKFDPTRFEGNGPAPYTFVPFGGGPRMCPGKEYARLEILVFMHNLVKRFKWEKVIPDEKIIVDPFPIPAKDLPIRLYPHKA