AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0573520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0573520 MtrunA17_Chr8g0389371 98.450 129 2 0 1 129 1 129 3.69e-94 267
Msa0573520 MtrunA17_Chr4g0051921 52.000 125 60 0 5 129 6 130 6.13e-46 145
Msa0573520 MtrunA17_Chr1g0205971 52.000 125 60 0 5 129 6 130 6.13e-46 145
Msa0573520 MtrunA17_Chr4g0065831 52.000 125 60 0 5 129 6 130 1.57e-45 144
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0573520 AT4G29430.1 81.395 129 24 0 1 129 1 129 6.01e-79 229
Msa0573520 AT2G19720.1 79.845 129 26 0 1 129 1 129 1.67e-77 225
Msa0573520 AT2G39590.1 52.000 125 60 0 5 129 6 130 3.64e-46 146
Msa0573520 AT5G59850.1 52.000 125 60 0 5 129 6 130 2.05e-45 144
Msa0573520 AT1G07770.2 52.000 125 60 0 5 129 6 130 2.05e-45 144
Msa0573520 AT1G07770.3 52.000 125 60 0 5 129 6 130 2.05e-45 144
Msa0573520 AT1G07770.1 52.000 125 60 0 5 129 6 130 2.05e-45 144
Msa0573520 AT3G46040.1 50.400 125 62 0 5 129 6 130 2.87e-44 141

Find 66 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTCGTTTACCTTGTTATTT+AGG 0.207524 4_1:-76743576 Msa0573520:three_prime_UTR
ATCACAATCTTTGTGGAAAT+TGG 0.217738 4_1:-76743413 Msa0573520:three_prime_UTR
CTGTGATCCTCCCCACTCTA+TGG 0.263185 4_1:+76743985 None:intergenic
GAAGAGATAACATTGGAAAT+AGG 0.265767 4_1:+76744643 None:intergenic
TACCGCGGTGTTTCATGATT+TGG 0.271491 4_1:+76744614 None:intergenic
CGGGAGTGAGAAAGAGAAAT+GGG 0.274957 4_1:-76744742 Msa0573520:exon
TACAACTCCTGATGGTGTTT+TGG 0.307095 4_1:-76743661 Msa0573520:CDS
AATGTAGGTGGTCAGGTTCT+TGG 0.331557 4_1:-76743615 Msa0573520:CDS
AAATCCATTGAAAGCTTATC+AGG 0.331884 4_1:+76743528 None:intergenic
TCGGGAGTGAGAAAGAGAAA+TGG 0.388879 4_1:-76744743 Msa0573520:exon
AGGATATATCAAGGACTTTC+AGG 0.389863 4_1:-76744018 Msa0573520:CDS
CCTTTGCCTTGAGGTCTTGT+CGG 0.398017 4_1:+76743919 None:intergenic
AAACCGTTCTAGCTTGGCTT+TGG 0.416322 4_1:-76744804 Msa0573520:five_prime_UTR
GTTTCCTGATAAGCTTTCAA+TGG 0.417059 4_1:-76743532 Msa0573520:three_prime_UTR
TGAGGTCTTGTCGGTACGTA+AGG 0.430606 4_1:+76743928 None:intergenic
ATCATGAAGAGGCTGCAAAA+AGG 0.449293 4_1:-76743638 Msa0573520:CDS
CTTGCAGCGTCGCGAGGTAG+CGG 0.455702 4_1:-76744880 Msa0573520:five_prime_UTR
TGTGATCCTCCCCACTCTAT+GGG 0.457890 4_1:+76743986 None:intergenic
TTCAACATTGATTATCCTCT+AGG 0.463340 4_1:+76743205 None:intergenic
TAGCGGTGCGTAACGAGATC+GGG 0.465899 4_1:-76744761 Msa0573520:five_prime_UTR
AATAATAGACTAGACTTGAA+TGG 0.465985 4_1:-76743376 Msa0573520:three_prime_UTR
CGAACAAAACCGTTCTAGCT+TGG 0.470138 4_1:-76744810 Msa0573520:five_prime_UTR
GAACGATGCGTTGAGGAGTA+TGG 0.472523 4_1:-76744707 Msa0573520:CDS
ATAGCGGTGCGTAACGAGAT+CGG 0.475250 4_1:-76744762 Msa0573520:five_prime_UTR
GAACACTCTTCCAACACATC+AGG 0.488180 4_1:-76743877 Msa0573520:intron
TCATGATCCAAAACACCATC+AGG 0.488311 4_1:+76743654 None:intergenic
GAGAAAAGAAGAGATAACAT+TGG 0.493444 4_1:+76744636 None:intergenic
CATTAACCAACCTGATGTGT+TGG 0.507241 4_1:+76743867 None:intergenic
GCGTTGGATCACAATCTTTG+TGG 0.531472 4_1:-76743420 Msa0573520:three_prime_UTR
AACTGCCACATAATTCATAC+AGG 0.539779 4_1:+76743453 None:intergenic
ATTCAATATCCTTTGCCTTG+AGG 0.544391 4_1:+76743910 None:intergenic
TATCCACCAGGCGACAAGGT+TGG 0.546287 4_1:+76743173 None:intergenic
TTTGTCCTGTATGAATTATG+TGG 0.561597 4_1:-76743458 Msa0573520:three_prime_UTR
GAAGAGAGGCAAAGCTATGG+TGG 0.562394 4_1:-76744674 Msa0573520:CDS
GCAGCGTCGCGAGGTAGCGG+CGG 0.568656 4_1:-76744877 Msa0573520:five_prime_UTR
AGATTACAACTAAGATGATA+CGG 0.572731 4_1:+76743350 None:intergenic
GCAGTTCATGCATGATGCGT+TGG 0.574386 4_1:-76743436 Msa0573520:three_prime_UTR
GTGTATGATCCCCATAGAGT+GGG 0.576109 4_1:-76743996 Msa0573520:CDS
ACGCCAAAGCCAAGCTAGAA+CGG 0.576207 4_1:+76744801 None:intergenic
GTTCGTTAATTGTGAAATAG+CGG 0.576806 4_1:+76744828 None:intergenic
CATGTTGGCAGGATATATCA+AGG 0.581279 4_1:-76744027 Msa0573520:intron
ACTTAGCTGAAGACAGTGAC+TGG 0.585077 4_1:+76743553 None:intergenic
GAGGTCTTGTCGGTACGTAA+GGG 0.585892 4_1:+76743929 None:intergenic
AGAAAACTAGTGTTGCAGAA+TGG 0.589693 4_1:+76743278 None:intergenic
GTTCAAAGAGGGTTGAAAGA+CGG 0.593795 4_1:-76744915 Msa0573520:five_prime_UTR
GGGAGTGAGAAAGAGAAATG+GGG 0.613232 4_1:-76744741 Msa0573520:exon
TGAGAAGAGAGGCAAAGCTA+TGG 0.613395 4_1:-76744677 Msa0573520:CDS
GGTGTATGATCCCCATAGAG+TGG 0.614660 4_1:-76743997 Msa0573520:CDS
AAAATATCCACCAGGCGACA+AGG 0.616897 4_1:+76743169 None:intergenic
AGGTAGAATTAACGATTGCA+AGG 0.617470 4_1:-76743952 Msa0573520:CDS
ATAGCGGTGTTCCCAGTGGT+CGG 0.630435 4_1:+76744844 None:intergenic
GAATATTGAACGATGCGTTG+AGG 0.643387 4_1:-76744714 Msa0573520:CDS
TACGTACCGACAAGACCTCA+AGG 0.647236 4_1:-76743925 Msa0573520:CDS
AGAGTGCTTGCAGCGTCGCG+AGG 0.649570 4_1:-76744886 Msa0573520:five_prime_UTR
CCGACAAGACCTCAAGGCAA+AGG 0.651183 4_1:-76743919 Msa0573520:CDS
AGGATCACAGTTGAACTACA+AGG 0.660202 4_1:-76743972 Msa0573520:CDS
AGAATAATATATCATCCTAG+AGG 0.668397 4_1:-76743220 Msa0573520:three_prime_UTR
ATGGTGAATGCTGAGAAGAG+AGG 0.672519 4_1:-76744688 Msa0573520:CDS
AGTGACTGGCCTAAATAACA+AGG 0.673320 4_1:+76743567 None:intergenic
GTTGTGATTACAACTCCTGA+TGG 0.673437 4_1:-76743669 Msa0573520:CDS
GTGATCCTCCCCACTCTATG+GGG 0.676832 4_1:+76743987 None:intergenic
TGTATGATCCCCATAGAGTG+GGG 0.683001 4_1:-76743995 Msa0573520:CDS
ACTGTTTGTGAAAACTATAG+CGG 0.693709 4_1:-76744778 Msa0573520:five_prime_UTR
ATGATCCCCATAGAGTGGGG+AGG 0.698167 4_1:-76743992 Msa0573520:CDS
TGAAATAGCGGTGTTCCCAG+TGG 0.707962 4_1:+76744840 None:intergenic
CTCCAAATCATGAAACACCG+CGG 0.727632 4_1:-76744616 Msa0573520:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTAAAGATATTTTTAG+TGG - chr4_1:76743762-76743781 Msa0573520:intron 10.0%
!!! ATTTTTAAAGATATTTTTAG+TGG - chr4_1:76743762-76743781 Msa0573520:intron 10.0%
!! AACACTAAATAAAAACAAAA+CGG + chr4_1:76744307-76744326 None:intergenic 15.0%
!! AACACTAAATAAAAACAAAA+CGG + chr4_1:76744307-76744326 None:intergenic 15.0%
!!! TTATGTATTTGTTTTTCTGA+TGG - chr4_1:76743619-76743638 Msa0573520:CDS 20.0%
!! TGTCTTAATTAAAGGAATTT+GGG + chr4_1:76743697-76743716 None:intergenic 20.0%
!! ATGTCTTAATTAAAGGAATT+TGG + chr4_1:76743698-76743717 None:intergenic 20.0%
!! CTAATGTATGTCTTAATTAA+AGG + chr4_1:76743705-76743724 None:intergenic 20.0%
!! CTTTAATTAAGACATACATT+AGG - chr4_1:76743703-76743722 Msa0573520:intron 20.0%
!!! CGAATTTAAAATATAAAAGC+TGG + chr4_1:76744379-76744398 None:intergenic 20.0%
!! TTACTAATAGATATGAGTAA+AGG - chr4_1:76744405-76744424 Msa0573520:intron 20.0%
!!! TTATGTATTTGTTTTTCTGA+TGG - chr4_1:76743619-76743638 Msa0573520:CDS 20.0%
!! TGTCTTAATTAAAGGAATTT+GGG + chr4_1:76743697-76743716 None:intergenic 20.0%
!! ATGTCTTAATTAAAGGAATT+TGG + chr4_1:76743698-76743717 None:intergenic 20.0%
!! CTAATGTATGTCTTAATTAA+AGG + chr4_1:76743705-76743724 None:intergenic 20.0%
!! CTTTAATTAAGACATACATT+AGG - chr4_1:76743703-76743722 Msa0573520:intron 20.0%
!!! CGAATTTAAAATATAAAAGC+TGG + chr4_1:76744379-76744398 None:intergenic 20.0%
!! TTACTAATAGATATGAGTAA+AGG - chr4_1:76744405-76744424 Msa0573520:intron 20.0%
! AGAGAAAAAAAAGTTCAAAG+AGG - chr4_1:76743217-76743236 Msa0573520:three_prime_UTR 25.0%
! GAGAAAAAAAAGTTCAAAGA+GGG - chr4_1:76743218-76743237 Msa0573520:three_prime_UTR 25.0%
! ATTTCAAACAAATATTACCG+CGG + chr4_1:76743548-76743567 None:intergenic 25.0%
! ACAAATATAATCACATGTGT+AGG + chr4_1:76743859-76743878 None:intergenic 25.0%
! TTACTTCCATTATCTCAAAT+TGG - chr4_1:76743879-76743898 Msa0573520:CDS 25.0%
! TCATATCTCATGAAAAGAAA+TGG - chr4_1:76743948-76743967 Msa0573520:CDS 25.0%
! TAATTGTTGGAAAATAACCA+TGG + chr4_1:76743977-76743996 None:intergenic 25.0%
! ATAGATATGAGTAAAGGAAA+AGG - chr4_1:76744411-76744430 Msa0573520:intron 25.0%
!!! TTTTATTTATCTTGATGCAG+TGG - chr4_1:76744446-76744465 Msa0573520:intron 25.0%
! TTTATTTATCTTGATGCAGT+GGG - chr4_1:76744447-76744466 Msa0573520:intron 25.0%
! AATAATAGACTAGACTTGAA+TGG - chr4_1:76744768-76744787 Msa0573520:five_prime_UTR 25.0%
! AGATTACAACTAAGATGATA+CGG + chr4_1:76744797-76744816 None:intergenic 25.0%
!!! TTTTCTTATTTGTTTTCGCA+AGG - chr4_1:76744883-76744902 Msa0573520:five_prime_UTR 25.0%
! AGAATAATATATCATCCTAG+AGG - chr4_1:76744924-76744943 Msa0573520:five_prime_UTR 25.0%
! AGAGAAAAAAAAGTTCAAAG+AGG - chr4_1:76743217-76743236 Msa0573520:three_prime_UTR 25.0%
! GAGAAAAAAAAGTTCAAAGA+GGG - chr4_1:76743218-76743237 Msa0573520:three_prime_UTR 25.0%
! ATTTCAAACAAATATTACCG+CGG + chr4_1:76743548-76743567 None:intergenic 25.0%
! ACAAATATAATCACATGTGT+AGG + chr4_1:76743859-76743878 None:intergenic 25.0%
! TTACTTCCATTATCTCAAAT+TGG - chr4_1:76743879-76743898 Msa0573520:CDS 25.0%
! TCATATCTCATGAAAAGAAA+TGG - chr4_1:76743948-76743967 Msa0573520:CDS 25.0%
! TAATTGTTGGAAAATAACCA+TGG + chr4_1:76743977-76743996 None:intergenic 25.0%
! ATAGATATGAGTAAAGGAAA+AGG - chr4_1:76744411-76744430 Msa0573520:intron 25.0%
!!! TTTTATTTATCTTGATGCAG+TGG - chr4_1:76744446-76744465 Msa0573520:intron 25.0%
! TTTATTTATCTTGATGCAGT+GGG - chr4_1:76744447-76744466 Msa0573520:intron 25.0%
! AATAATAGACTAGACTTGAA+TGG - chr4_1:76744768-76744787 Msa0573520:five_prime_UTR 25.0%
! AGATTACAACTAAGATGATA+CGG + chr4_1:76744797-76744816 None:intergenic 25.0%
!!! TTTTCTTATTTGTTTTCGCA+AGG - chr4_1:76744883-76744902 Msa0573520:five_prime_UTR 25.0%
! AGAATAATATATCATCCTAG+AGG - chr4_1:76744924-76744943 Msa0573520:five_prime_UTR 25.0%
GTTCGTTAATTGTGAAATAG+CGG + chr4_1:76743319-76743338 None:intergenic 30.0%
ACTGTTTGTGAAAACTATAG+CGG - chr4_1:76743366-76743385 Msa0573520:three_prime_UTR 30.0%
GAAGAGATAACATTGGAAAT+AGG + chr4_1:76743504-76743523 None:intergenic 30.0%
GAGAAAAGAAGAGATAACAT+TGG + chr4_1:76743511-76743530 None:intergenic 30.0%
TTTCATACTCACAAGATGAA+GGG + chr4_1:76743598-76743617 None:intergenic 30.0%
!!! TCTGATGGATTTATGTGTTT+TGG - chr4_1:76743634-76743653 Msa0573520:CDS 30.0%
! GTCATACCAATTTGAGATAA+TGG + chr4_1:76743888-76743907 None:intergenic 30.0%
! ATTTTCCAACAATTATCAGC+TGG - chr4_1:76743982-76744001 Msa0573520:CDS 30.0%
AGCATGTATATGTACATGAA+AGG - chr4_1:76744033-76744052 Msa0573520:intron 30.0%
CAGTTTCACCTATTTGTATA+TGG - chr4_1:76744331-76744350 Msa0573520:intron 30.0%
AGCATTCTCCATATACAAAT+AGG + chr4_1:76744342-76744361 None:intergenic 30.0%
TTATTTATCTTGATGCAGTG+GGG - chr4_1:76744448-76744467 Msa0573520:intron 30.0%
!! TGTCGTTTACCTTGTTATTT+AGG - chr4_1:76744568-76744587 Msa0573520:intron 30.0%
AAATCCATTGAAAGCTTATC+AGG + chr4_1:76744619-76744638 None:intergenic 30.0%
! TGAAAGAATGTTTTCAGCAA+AGG - chr4_1:76744641-76744660 Msa0573520:CDS 30.0%
TTTGTCCTGTATGAATTATG+TGG - chr4_1:76744686-76744705 Msa0573520:CDS 30.0%
ATCACAATCTTTGTGGAAAT+TGG - chr4_1:76744731-76744750 Msa0573520:exon 30.0%
! TTCAACATTGATTATCCTCT+AGG + chr4_1:76744942-76744961 None:intergenic 30.0%
GTTCGTTAATTGTGAAATAG+CGG + chr4_1:76743319-76743338 None:intergenic 30.0%
ACTGTTTGTGAAAACTATAG+CGG - chr4_1:76743366-76743385 Msa0573520:three_prime_UTR 30.0%
GAAGAGATAACATTGGAAAT+AGG + chr4_1:76743504-76743523 None:intergenic 30.0%
GAGAAAAGAAGAGATAACAT+TGG + chr4_1:76743511-76743530 None:intergenic 30.0%
TTTCATACTCACAAGATGAA+GGG + chr4_1:76743598-76743617 None:intergenic 30.0%
!!! TCTGATGGATTTATGTGTTT+TGG - chr4_1:76743634-76743653 Msa0573520:CDS 30.0%
! GTCATACCAATTTGAGATAA+TGG + chr4_1:76743888-76743907 None:intergenic 30.0%
! ATTTTCCAACAATTATCAGC+TGG - chr4_1:76743982-76744001 Msa0573520:CDS 30.0%
AGCATGTATATGTACATGAA+AGG - chr4_1:76744033-76744052 Msa0573520:intron 30.0%
CAGTTTCACCTATTTGTATA+TGG - chr4_1:76744331-76744350 Msa0573520:intron 30.0%
AGCATTCTCCATATACAAAT+AGG + chr4_1:76744342-76744361 None:intergenic 30.0%
TTATTTATCTTGATGCAGTG+GGG - chr4_1:76744448-76744467 Msa0573520:intron 30.0%
!! TGTCGTTTACCTTGTTATTT+AGG - chr4_1:76744568-76744587 Msa0573520:intron 30.0%
AAATCCATTGAAAGCTTATC+AGG + chr4_1:76744619-76744638 None:intergenic 30.0%
! TGAAAGAATGTTTTCAGCAA+AGG - chr4_1:76744641-76744660 Msa0573520:CDS 30.0%
TTTGTCCTGTATGAATTATG+TGG - chr4_1:76744686-76744705 Msa0573520:CDS 30.0%
ATCACAATCTTTGTGGAAAT+TGG - chr4_1:76744731-76744750 Msa0573520:exon 30.0%
! TTCAACATTGATTATCCTCT+AGG + chr4_1:76744942-76744961 None:intergenic 30.0%
CTTTCATACTCACAAGATGA+AGG + chr4_1:76743599-76743618 None:intergenic 35.0%
!!! TGTGTTTTGGCGAATTCATT+AGG - chr4_1:76743647-76743666 Msa0573520:CDS 35.0%
GCTACATAGTTCTTGAGAAA+TGG + chr4_1:76743796-76743815 None:intergenic 35.0%
GCGTATACACTTTAGATTGA+AGG - chr4_1:76743814-76743833 Msa0573520:intron 35.0%
ATGAAAAGAAATGGTCTCCA+TGG - chr4_1:76743957-76743976 Msa0573520:CDS 35.0%
CTTATCCAGCTGATAATTGT+TGG + chr4_1:76743990-76744009 None:intergenic 35.0%
AATTATCAGCTGGATAAGCA+TGG - chr4_1:76743992-76744011 Msa0573520:CDS 35.0%
!! TAATTTGCTGTCGTTCATGT+TGG - chr4_1:76744102-76744121 Msa0573520:intron 35.0%
AGGATATATCAAGGACTTTC+AGG - chr4_1:76744126-76744145 Msa0573520:intron 35.0%
AGGTAGAATTAACGATTGCA+AGG - chr4_1:76744192-76744211 Msa0573520:intron 35.0%
ATTCAATATCCTTTGCCTTG+AGG + chr4_1:76744237-76744256 None:intergenic 35.0%
GTTTCCTGATAAGCTTTCAA+TGG - chr4_1:76744612-76744631 Msa0573520:intron 35.0%
! AAGAATGTTTTCAGCAAAGG+AGG - chr4_1:76744644-76744663 Msa0573520:CDS 35.0%
AACTGCCACATAATTCATAC+AGG + chr4_1:76744694-76744713 None:intergenic 35.0%
AGAAAACTAGTGTTGCAGAA+TGG + chr4_1:76744869-76744888 None:intergenic 35.0%
CTTTCATACTCACAAGATGA+AGG + chr4_1:76743599-76743618 None:intergenic 35.0%
!!! TGTGTTTTGGCGAATTCATT+AGG - chr4_1:76743647-76743666 Msa0573520:CDS 35.0%
GCTACATAGTTCTTGAGAAA+TGG + chr4_1:76743796-76743815 None:intergenic 35.0%
GCGTATACACTTTAGATTGA+AGG - chr4_1:76743814-76743833 Msa0573520:intron 35.0%
ATGAAAAGAAATGGTCTCCA+TGG - chr4_1:76743957-76743976 Msa0573520:CDS 35.0%
CTTATCCAGCTGATAATTGT+TGG + chr4_1:76743990-76744009 None:intergenic 35.0%
AATTATCAGCTGGATAAGCA+TGG - chr4_1:76743992-76744011 Msa0573520:CDS 35.0%
!! TAATTTGCTGTCGTTCATGT+TGG - chr4_1:76744102-76744121 Msa0573520:intron 35.0%
AGGATATATCAAGGACTTTC+AGG - chr4_1:76744126-76744145 Msa0573520:intron 35.0%
AGGTAGAATTAACGATTGCA+AGG - chr4_1:76744192-76744211 Msa0573520:intron 35.0%
ATTCAATATCCTTTGCCTTG+AGG + chr4_1:76744237-76744256 None:intergenic 35.0%
GTTTCCTGATAAGCTTTCAA+TGG - chr4_1:76744612-76744631 Msa0573520:intron 35.0%
! AAGAATGTTTTCAGCAAAGG+AGG - chr4_1:76744644-76744663 Msa0573520:CDS 35.0%
AACTGCCACATAATTCATAC+AGG + chr4_1:76744694-76744713 None:intergenic 35.0%
AGAAAACTAGTGTTGCAGAA+TGG + chr4_1:76744869-76744888 None:intergenic 35.0%
GTTCAAAGAGGGTTGAAAGA+CGG - chr4_1:76743229-76743248 Msa0573520:three_prime_UTR 40.0%
GAATATTGAACGATGCGTTG+AGG - chr4_1:76743430-76743449 Msa0573520:three_prime_UTR 40.0%
CTCAAATTGGTATGACGTTG+AGG - chr4_1:76743892-76743911 Msa0573520:CDS 40.0%
CATGTTGGCAGGATATATCA+AGG - chr4_1:76744117-76744136 Msa0573520:intron 40.0%
AGGATCACAGTTGAACTACA+AGG - chr4_1:76744172-76744191 Msa0573520:intron 40.0%
CATTAACCAACCTGATGTGT+TGG + chr4_1:76744280-76744299 None:intergenic 40.0%
GTTGTGATTACAACTCCTGA+TGG - chr4_1:76744475-76744494 Msa0573520:intron 40.0%
!! TACAACTCCTGATGGTGTTT+TGG - chr4_1:76744483-76744502 Msa0573520:intron 40.0%
TCATGATCCAAAACACCATC+AGG + chr4_1:76744493-76744512 None:intergenic 40.0%
!! TGGTGTTTTGGATCATGAAG+AGG - chr4_1:76744495-76744514 Msa0573520:intron 40.0%
ATCATGAAGAGGCTGCAAAA+AGG - chr4_1:76744506-76744525 Msa0573520:intron 40.0%
AAAAAGGAATGTAGGTGGTC+AGG - chr4_1:76744522-76744541 Msa0573520:intron 40.0%
!! AGTGACTGGCCTAAATAACA+AGG + chr4_1:76744580-76744599 None:intergenic 40.0%
GTTCAAAGAGGGTTGAAAGA+CGG - chr4_1:76743229-76743248 Msa0573520:three_prime_UTR 40.0%
GAATATTGAACGATGCGTTG+AGG - chr4_1:76743430-76743449 Msa0573520:three_prime_UTR 40.0%
CTCAAATTGGTATGACGTTG+AGG - chr4_1:76743892-76743911 Msa0573520:CDS 40.0%
CATGTTGGCAGGATATATCA+AGG - chr4_1:76744117-76744136 Msa0573520:intron 40.0%
AGGATCACAGTTGAACTACA+AGG - chr4_1:76744172-76744191 Msa0573520:intron 40.0%
CATTAACCAACCTGATGTGT+TGG + chr4_1:76744280-76744299 None:intergenic 40.0%
GTTGTGATTACAACTCCTGA+TGG - chr4_1:76744475-76744494 Msa0573520:intron 40.0%
!! TACAACTCCTGATGGTGTTT+TGG - chr4_1:76744483-76744502 Msa0573520:intron 40.0%
TCATGATCCAAAACACCATC+AGG + chr4_1:76744493-76744512 None:intergenic 40.0%
!! TGGTGTTTTGGATCATGAAG+AGG - chr4_1:76744495-76744514 Msa0573520:intron 40.0%
ATCATGAAGAGGCTGCAAAA+AGG - chr4_1:76744506-76744525 Msa0573520:intron 40.0%
AAAAAGGAATGTAGGTGGTC+AGG - chr4_1:76744522-76744541 Msa0573520:intron 40.0%
!! AGTGACTGGCCTAAATAACA+AGG + chr4_1:76744580-76744599 None:intergenic 40.0%
! CGAACAAAACCGTTCTAGCT+TGG - chr4_1:76743334-76743353 Msa0573520:three_prime_UTR 45.0%
!! AAACCGTTCTAGCTTGGCTT+TGG - chr4_1:76743340-76743359 Msa0573520:three_prime_UTR 45.0%
TCGGGAGTGAGAAAGAGAAA+TGG - chr4_1:76743401-76743420 Msa0573520:three_prime_UTR 45.0%
CGGGAGTGAGAAAGAGAAAT+GGG - chr4_1:76743402-76743421 Msa0573520:three_prime_UTR 45.0%
GGGAGTGAGAAAGAGAAATG+GGG - chr4_1:76743403-76743422 Msa0573520:three_prime_UTR 45.0%
ATGGTGAATGCTGAGAAGAG+AGG - chr4_1:76743456-76743475 Msa0573520:three_prime_UTR 45.0%
TGAGAAGAGAGGCAAAGCTA+TGG - chr4_1:76743467-76743486 Msa0573520:three_prime_UTR 45.0%
CTCCAAATCATGAAACACCG+CGG - chr4_1:76743528-76743547 Msa0573520:three_prime_UTR 45.0%
TACCGCGGTGTTTCATGATT+TGG + chr4_1:76743533-76743552 None:intergenic 45.0%
ATTGGTATGACGTTGAGGTG+TGG - chr4_1:76743897-76743916 Msa0573520:CDS 45.0%
TTGGTATGACGTTGAGGTGT+GGG - chr4_1:76743898-76743917 Msa0573520:CDS 45.0%
GTGTATGATCCCCATAGAGT+GGG - chr4_1:76744148-76744167 Msa0573520:intron 45.0%
TGTATGATCCCCATAGAGTG+GGG - chr4_1:76744149-76744168 Msa0573520:intron 45.0%
GAACACTCTTCCAACACATC+AGG - chr4_1:76744267-76744286 Msa0573520:intron 45.0%
ACTCTTCCAACACATCAGGT+TGG - chr4_1:76744271-76744290 Msa0573520:intron 45.0%
GAGGCTGCAAAAAGGAATGT+AGG - chr4_1:76744514-76744533 Msa0573520:intron 45.0%
GCTGCAAAAAGGAATGTAGG+TGG - chr4_1:76744517-76744536 Msa0573520:intron 45.0%
AATGTAGGTGGTCAGGTTCT+TGG - chr4_1:76744529-76744548 Msa0573520:intron 45.0%
ACTTAGCTGAAGACAGTGAC+TGG + chr4_1:76744594-76744613 None:intergenic 45.0%
GCGTTGGATCACAATCTTTG+TGG - chr4_1:76744724-76744743 Msa0573520:CDS 45.0%
! CGAACAAAACCGTTCTAGCT+TGG - chr4_1:76743334-76743353 Msa0573520:three_prime_UTR 45.0%
!! AAACCGTTCTAGCTTGGCTT+TGG - chr4_1:76743340-76743359 Msa0573520:three_prime_UTR 45.0%
TCGGGAGTGAGAAAGAGAAA+TGG - chr4_1:76743401-76743420 Msa0573520:three_prime_UTR 45.0%
CGGGAGTGAGAAAGAGAAAT+GGG - chr4_1:76743402-76743421 Msa0573520:three_prime_UTR 45.0%
GGGAGTGAGAAAGAGAAATG+GGG - chr4_1:76743403-76743422 Msa0573520:three_prime_UTR 45.0%
ATGGTGAATGCTGAGAAGAG+AGG - chr4_1:76743456-76743475 Msa0573520:three_prime_UTR 45.0%
TGAGAAGAGAGGCAAAGCTA+TGG - chr4_1:76743467-76743486 Msa0573520:three_prime_UTR 45.0%
CTCCAAATCATGAAACACCG+CGG - chr4_1:76743528-76743547 Msa0573520:three_prime_UTR 45.0%
TACCGCGGTGTTTCATGATT+TGG + chr4_1:76743533-76743552 None:intergenic 45.0%
ATTGGTATGACGTTGAGGTG+TGG - chr4_1:76743897-76743916 Msa0573520:CDS 45.0%
TTGGTATGACGTTGAGGTGT+GGG - chr4_1:76743898-76743917 Msa0573520:CDS 45.0%
GTGTATGATCCCCATAGAGT+GGG - chr4_1:76744148-76744167 Msa0573520:intron 45.0%
TGTATGATCCCCATAGAGTG+GGG - chr4_1:76744149-76744168 Msa0573520:intron 45.0%
GAACACTCTTCCAACACATC+AGG - chr4_1:76744267-76744286 Msa0573520:intron 45.0%
ACTCTTCCAACACATCAGGT+TGG - chr4_1:76744271-76744290 Msa0573520:intron 45.0%
GAGGCTGCAAAAAGGAATGT+AGG - chr4_1:76744514-76744533 Msa0573520:intron 45.0%
GCTGCAAAAAGGAATGTAGG+TGG - chr4_1:76744517-76744536 Msa0573520:intron 45.0%
AATGTAGGTGGTCAGGTTCT+TGG - chr4_1:76744529-76744548 Msa0573520:intron 45.0%
ACTTAGCTGAAGACAGTGAC+TGG + chr4_1:76744594-76744613 None:intergenic 45.0%
GCGTTGGATCACAATCTTTG+TGG - chr4_1:76744724-76744743 Msa0573520:CDS 45.0%
!!! TATTATTAATTTTAGATTTT+TGG - chr4_1:76743727-76743746 Msa0573520:intron 5.0%
!!! TATTATTAATTTTAGATTTT+TGG - chr4_1:76743727-76743746 Msa0573520:intron 5.0%
! GCGAACATTTTCCGACCACT+GGG - chr4_1:76743289-76743308 Msa0573520:three_prime_UTR 50.0%
TGAAATAGCGGTGTTCCCAG+TGG + chr4_1:76743307-76743326 None:intergenic 50.0%
ACGCCAAAGCCAAGCTAGAA+CGG + chr4_1:76743346-76743365 None:intergenic 50.0%
ATAGCGGTGCGTAACGAGAT+CGG - chr4_1:76743382-76743401 Msa0573520:three_prime_UTR 50.0%
GAACGATGCGTTGAGGAGTA+TGG - chr4_1:76743437-76743456 Msa0573520:three_prime_UTR 50.0%
GAAGAGAGGCAAAGCTATGG+TGG - chr4_1:76743470-76743489 Msa0573520:three_prime_UTR 50.0%
TTGCTGTCGTTCATGTTGGC+AGG - chr4_1:76744106-76744125 Msa0573520:intron 50.0%
GGTGTATGATCCCCATAGAG+TGG - chr4_1:76744147-76744166 Msa0573520:intron 50.0%
TGTGATCCTCCCCACTCTAT+GGG + chr4_1:76744161-76744180 None:intergenic 50.0%
! GAGGTCTTGTCGGTACGTAA+GGG + chr4_1:76744218-76744237 None:intergenic 50.0%
! TGAGGTCTTGTCGGTACGTA+AGG + chr4_1:76744219-76744238 None:intergenic 50.0%
! TACGTACCGACAAGACCTCA+AGG - chr4_1:76744219-76744238 Msa0573520:intron 50.0%
! CCTTTGCCTTGAGGTCTTGT+CGG + chr4_1:76744228-76744247 None:intergenic 50.0%
GCAGTTCATGCATGATGCGT+TGG - chr4_1:76744708-76744727 Msa0573520:CDS 50.0%
! GCGAACATTTTCCGACCACT+GGG - chr4_1:76743289-76743308 Msa0573520:three_prime_UTR 50.0%
TGAAATAGCGGTGTTCCCAG+TGG + chr4_1:76743307-76743326 None:intergenic 50.0%
ACGCCAAAGCCAAGCTAGAA+CGG + chr4_1:76743346-76743365 None:intergenic 50.0%
ATAGCGGTGCGTAACGAGAT+CGG - chr4_1:76743382-76743401 Msa0573520:three_prime_UTR 50.0%
GAACGATGCGTTGAGGAGTA+TGG - chr4_1:76743437-76743456 Msa0573520:three_prime_UTR 50.0%
GAAGAGAGGCAAAGCTATGG+TGG - chr4_1:76743470-76743489 Msa0573520:three_prime_UTR 50.0%
TTGCTGTCGTTCATGTTGGC+AGG - chr4_1:76744106-76744125 Msa0573520:intron 50.0%
GGTGTATGATCCCCATAGAG+TGG - chr4_1:76744147-76744166 Msa0573520:intron 50.0%
TGTGATCCTCCCCACTCTAT+GGG + chr4_1:76744161-76744180 None:intergenic 50.0%
! GAGGTCTTGTCGGTACGTAA+GGG + chr4_1:76744218-76744237 None:intergenic 50.0%
! TGAGGTCTTGTCGGTACGTA+AGG + chr4_1:76744219-76744238 None:intergenic 50.0%
! TACGTACCGACAAGACCTCA+AGG - chr4_1:76744219-76744238 Msa0573520:intron 50.0%
! CCTTTGCCTTGAGGTCTTGT+CGG + chr4_1:76744228-76744247 None:intergenic 50.0%
GCAGTTCATGCATGATGCGT+TGG - chr4_1:76744708-76744727 Msa0573520:CDS 50.0%
! GGCGAACATTTTCCGACCAC+TGG - chr4_1:76743288-76743307 Msa0573520:three_prime_UTR 55.0%
ATAGCGGTGTTCCCAGTGGT+CGG + chr4_1:76743303-76743322 None:intergenic 55.0%
TAGCGGTGCGTAACGAGATC+GGG - chr4_1:76743383-76743402 Msa0573520:three_prime_UTR 55.0%
ATGATCCCCATAGAGTGGGG+AGG - chr4_1:76744152-76744171 Msa0573520:intron 55.0%
GTGATCCTCCCCACTCTATG+GGG + chr4_1:76744160-76744179 None:intergenic 55.0%
CTGTGATCCTCCCCACTCTA+TGG + chr4_1:76744162-76744181 None:intergenic 55.0%
CCGACAAGACCTCAAGGCAA+AGG - chr4_1:76744225-76744244 Msa0573520:intron 55.0%
! GGCGAACATTTTCCGACCAC+TGG - chr4_1:76743288-76743307 Msa0573520:three_prime_UTR 55.0%
ATAGCGGTGTTCCCAGTGGT+CGG + chr4_1:76743303-76743322 None:intergenic 55.0%
TAGCGGTGCGTAACGAGATC+GGG - chr4_1:76743383-76743402 Msa0573520:three_prime_UTR 55.0%
ATGATCCCCATAGAGTGGGG+AGG - chr4_1:76744152-76744171 Msa0573520:intron 55.0%
GTGATCCTCCCCACTCTATG+GGG + chr4_1:76744160-76744179 None:intergenic 55.0%
CTGTGATCCTCCCCACTCTA+TGG + chr4_1:76744162-76744181 None:intergenic 55.0%
CCGACAAGACCTCAAGGCAA+AGG - chr4_1:76744225-76744244 Msa0573520:intron 55.0%
! AGAGTGCTTGCAGCGTCGCG+AGG - chr4_1:76743258-76743277 Msa0573520:three_prime_UTR 65.0%
CTTGCAGCGTCGCGAGGTAG+CGG - chr4_1:76743264-76743283 Msa0573520:three_prime_UTR 65.0%
! AGAGTGCTTGCAGCGTCGCG+AGG - chr4_1:76743258-76743277 Msa0573520:three_prime_UTR 65.0%
CTTGCAGCGTCGCGAGGTAG+CGG - chr4_1:76743264-76743283 Msa0573520:three_prime_UTR 65.0%
! GCAGCGTCGCGAGGTAGCGG+CGG - chr4_1:76743267-76743286 Msa0573520:three_prime_UTR 75.0%
! GCAGCGTCGCGAGGTAGCGG+CGG - chr4_1:76743267-76743286 Msa0573520:three_prime_UTR 75.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 76743180 76744986 76743180 ID=Msa0573520;Name=Msa0573520
chr4_1 mRNA 76743180 76744986 76743180 ID=Msa0573520-mRNA-1;Parent=Msa0573520;Name=Msa0573520-mRNA-1;_AED=0.02;_eAED=0.02;_QI=240|1|1|1|1|1|3|422|129
chr4_1 exon 76743180 76743700 76743180 ID=Msa0573520-mRNA-1:exon:26915;Parent=Msa0573520-mRNA-1
chr4_1 exon 76743878 76744038 76743878 ID=Msa0573520-mRNA-1:exon:26914;Parent=Msa0573520-mRNA-1
chr4_1 exon 76744617 76744986 76744617 ID=Msa0573520-mRNA-1:exon:26913;Parent=Msa0573520-mRNA-1
chr4_1 five_prime_UTR 76744747 76744986 76744747 ID=Msa0573520-mRNA-1:five_prime_utr;Parent=Msa0573520-mRNA-1
chr4_1 CDS 76744617 76744746 76744617 ID=Msa0573520-mRNA-1:cds;Parent=Msa0573520-mRNA-1
chr4_1 CDS 76743878 76744038 76743878 ID=Msa0573520-mRNA-1:cds;Parent=Msa0573520-mRNA-1
chr4_1 CDS 76743602 76743700 76743602 ID=Msa0573520-mRNA-1:cds;Parent=Msa0573520-mRNA-1
chr4_1 three_prime_UTR 76743180 76743601 76743180 ID=Msa0573520-mRNA-1:three_prime_utr;Parent=Msa0573520-mRNA-1
Gene Sequence

>Msa0573520

ATGGGGAGAAGAATATTGAACGATGCGTTGAGGAGTATGGTGAATGCTGAGAAGAGAGGCAAAGCTATGGTGGAGCTTAAACCTATTTCCAATGTTATCTCTTCTTTTCTCCAAATCATGAAACACCGCGGATATATCAAGGACTTTCAGGTGTATGATCCCCATAGAGTGGGGAGGATCACAGTTGAACTACAAGGTAGAATTAACGATTGCAAGGCCCTTACGTACCGACAAGACCTCAAGGCAAAGGATATTGAATCTTACAGATTGAACACTCTTCCAACACATCAGTGGGGTTATGTTGTGATTACAACTCCTGATGGTGTTTTGGATCATGAAGAGGCTGCAAAAAGGAATGTAGGTGGTCAGGTTCTTGGTTACTTTCACTAA
Protein sequence

>Msa0573520

MGRRILNDALRSMVNAEKRGKAMVELKPISNVISSFLQIMKHRGYIKDFQVYDPHRVGRITVELQGRINDCKALTYRQDLKAKDIESYRLNTLPTHQWGYVVITTPDGVLDHEEAAKRNVGGQVLGYFH*