AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0573530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0573530 MtrunA17_Chr8g0389391 93.796 274 17 0 48 321 1 274 1.80e-176 495
Msa0573530 MtrunA17_Chr8g0389401 88.776 98 11 0 359 456 1 98 1.03e-55 180
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0573530 AT4G29420.1 45.952 457 231 6 1 452 1 446 3.76e-134 394

Find 135 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCTCTCTGTTTCTGATTTC+TGG -0.102421 4_1:+76746822 Msa0573530:CDS
CTTAAGGAACTTAATCTTAT+TGG 0.148001 4_1:+76747915 Msa0573530:CDS
TCTGAACATATGTATATTCT+TGG 0.209320 4_1:+76748979 Msa0573530:three_prime_UTR
GATGGCATCTAGTGTGTTTA+AGG 0.212657 4_1:+76748674 Msa0573530:exon
CGCAGGGTAAGTTTGGAAAA+TGG 0.222783 4_1:+76748404 Msa0573530:CDS
TTCAAATGCTCCTCTTTCTT+TGG 0.250922 4_1:+76748001 Msa0573530:CDS
TTAGATGGAGATGGGGAATA+TGG 0.260868 4_1:+76748621 Msa0573530:CDS
TTCTTTCCGCAGGGTAAGTT+TGG 0.275948 4_1:+76748397 Msa0573530:CDS
ATTTCTGGGTTCAATCTTGT+TGG 0.315218 4_1:+76746837 Msa0573530:CDS
GCTCTCTGTTTCTGATTTCT+GGG 0.347427 4_1:+76746823 Msa0573530:CDS
AACATAAGGTATCTAAGTTT+AGG 0.356561 4_1:+76748347 Msa0573530:CDS
AGGCTATGGTTTGGTGCAAT+TGG 0.363155 4_1:+76747767 Msa0573530:intron
AGATTAAGTTCCTTAAGGTT+AGG 0.364458 4_1:-76747909 None:intergenic
ACAGAGAGAGTTGTAGAGGT+TGG 0.367243 4_1:+76748272 Msa0573530:CDS
TACAAACTCAAGAGTCAAAT+TGG 0.367246 4_1:-76747844 None:intergenic
TACTTGTTTCCCTAACCTTA+AGG 0.368816 4_1:+76747899 Msa0573530:CDS
AACGCCACTTCTAGTTAATC+TGG 0.369392 4_1:-76748886 None:intergenic
ATGGTGACGGATTCGAGATT+GGG 0.377126 4_1:-76746674 None:intergenic
CAATAAGATTAAGTTCCTTA+AGG 0.377361 4_1:-76747914 None:intergenic
AGAGAGCGACTTAATCGAAT+TGG 0.381163 4_1:-76746807 None:intergenic
AAACTTAGATACCTTATGTT+GGG 0.381371 4_1:-76748344 None:intergenic
GTTTGGTGCAATTGGAGGTT+AGG 0.383923 4_1:+76747775 Msa0573530:CDS
ATTTCAATTTCATTCAAGAT+TGG 0.385697 4_1:+76746771 Msa0573530:CDS
GACTCTTGAGTTTGTAAGAT+TGG 0.385958 4_1:+76747851 Msa0573530:CDS
GTTGATCAAGTAGGAGAGTT+TGG 0.393214 4_1:+76748296 Msa0573530:CDS
GTGATGAGGTGCTTGGTATC+TGG 0.398273 4_1:-76746611 None:intergenic
GATTAAGTTCCTTAAGGTTA+GGG 0.398337 4_1:-76747908 None:intergenic
GATGCTGATCCTTGTGCTGC+TGG 0.400589 4_1:+76748560 Msa0573530:CDS
ATCTAGTGTGTTTAAGGAAA+AGG 0.413335 4_1:+76748680 Msa0573530:exon
GTCGGTCTTTGAATTGTTGT+AGG 0.416315 4_1:+76748707 Msa0573530:three_prime_UTR
AGAAGGAATCAAGGATACTT+TGG 0.419448 4_1:+76748646 Msa0573530:CDS
GCAAATTCCCTGGAGTTAGA+TGG 0.420453 4_1:+76748606 Msa0573530:CDS
TGAATTATTTCCCCAACATA+AGG 0.424575 4_1:+76748333 Msa0573530:CDS
GGTGTTAGAAATTCCTTCAA+TGG 0.424696 4_1:+76746424 Msa0573530:exon
AGGTGTGAGAGATTAATCTT+AGG 0.425607 4_1:-76747957 None:intergenic
GTATCTAAGTTTAGGTTATG+AGG 0.429251 4_1:+76748355 Msa0573530:CDS
CCGACCATTGACAAGTTTGA+AGG 0.432550 4_1:-76747977 None:intergenic
TTACAGATTGTGTTACAGTT+AGG 0.435675 4_1:+76748736 Msa0573530:three_prime_UTR
GCAGGGTAAGTTTGGAAAAT+GGG 0.438676 4_1:+76748405 Msa0573530:CDS
GATGGTGACGGATTCGAGAT+TGG 0.439990 4_1:-76746675 None:intergenic
TTTGGATAAATGCACCCTCC+TGG 0.440415 4_1:+76748511 Msa0573530:CDS
TAAGTTTAGGTTATGAGGCT+TGG 0.443271 4_1:+76748360 Msa0573530:CDS
GAACTTAATCTTATTGGTGT+TGG 0.446999 4_1:+76747921 Msa0573530:CDS
CCCCATCTCCATCTAACTCC+AGG 0.448635 4_1:-76748614 None:intergenic
CTTAATCTTATTGGTGTTGG+AGG 0.450290 4_1:+76747924 Msa0573530:CDS
CAAGAGTAAACTCAATTTCA+AGG 0.453685 4_1:-76748472 None:intergenic
ATCTGATGATCTTCACCTTA+CGG 0.465937 4_1:+76746748 Msa0573530:CDS
AGTTTGAACTACACTTGAAA+AGG 0.469623 4_1:-76748857 None:intergenic
TTGACAAAGAAGGTGCTTCA+AGG 0.471175 4_1:-76748085 None:intergenic
TTTCAAGTGTAGTTCAAACT+AGG 0.475252 4_1:+76748860 Msa0573530:three_prime_UTR
GAGGTTGGAGTTGATCAAGT+AGG 0.483396 4_1:+76748287 Msa0573530:CDS
TTGTGCTACAGGCTATGGTT+TGG 0.489604 4_1:+76747758 Msa0573530:intron
CCCTGGAGTTAGATGGAGAT+GGG 0.491028 4_1:+76748613 Msa0573530:CDS
CTATGGTTTGGTGCAATTGG+AGG 0.491781 4_1:+76747770 Msa0573530:CDS
TTCGGAGGAAGCGGAGTTGA+GGG 0.493295 4_1:-76746534 None:intergenic
TCTAGTGTGTTTAAGGAAAA+GGG 0.495016 4_1:+76748681 Msa0573530:exon
AAGAACAGAGAGAGTTGTAG+AGG 0.501443 4_1:+76748268 Msa0573530:CDS
GAGAAGGAGGTTCTTGAAAA+CGG 0.503343 4_1:-76746645 None:intergenic
TGATGGAGAGTTCTTTCCGC+AGG 0.504774 4_1:+76748387 Msa0573530:CDS
GAGGGTAGATTCTCCATTGA+AGG 0.510419 4_1:-76746437 None:intergenic
CGCATTCCGCTTCAAGCTGA+AGG 0.511737 4_1:-76748172 None:intergenic
AAGGATACTTTGGTTTCAGA+TGG 0.512935 4_1:+76748656 Msa0573530:CDS
TAAACTTAGATACCTTATGT+TGG 0.515466 4_1:-76748345 None:intergenic
CTTCGGAGGAAGCGGAGTTG+AGG 0.516753 4_1:-76746535 None:intergenic
TTGGATAAATGCACCCTCCT+GGG 0.518672 4_1:+76748512 Msa0573530:CDS
AACGCTTGGTTAGCTGTGGA+TGG 0.519585 4_1:+76747798 Msa0573530:CDS
CGAACGAACTTCGGAGGAAG+CGG 0.523808 4_1:-76746543 None:intergenic
AATATGGAAAGAAGGAATCA+AGG 0.525029 4_1:+76748637 Msa0573530:CDS
CACACCTTCAAACTTGTCAA+TGG 0.525428 4_1:+76747973 Msa0573530:CDS
ATTTCTACAACCAGCGGAGA+GGG 0.527269 4_1:-76746455 None:intergenic
TTCATCTTCAAAGTCGTCAA+AGG 0.528968 4_1:-76746726 None:intergenic
AGATGGAAATCAACAAGACT+TGG 0.529529 4_1:-76748035 None:intergenic
GAGGGTCAACGAACGAACTT+CGG 0.530729 4_1:-76746552 None:intergenic
ACGATCAACGCCGATGGTGA+CGG 0.532340 4_1:-76746687 None:intergenic
CCTTCAAACTTGTCAATGGT+CGG 0.533893 4_1:+76747977 Msa0573530:CDS
TGATTTGAGGTAACGAGAGA+TGG 0.534176 4_1:-76746582 None:intergenic
ATTGTGTTACAGTTAGGAGT+AGG 0.538559 4_1:+76748742 Msa0573530:three_prime_UTR
CTAACAAGTGGAAGAGATGA+GGG 0.540516 4_1:-76746879 None:intergenic
GAGTCTGCATCTTGCTAGAT+CGG 0.541109 4_1:-76746501 None:intergenic
TTATGAGGCTTGGCTTGTGA+TGG 0.541430 4_1:+76748370 Msa0573530:CDS
GGAGAATCTACCCTCTCCGC+TGG 0.541471 4_1:+76746445 Msa0573530:CDS
AGATGGGGAATATGGAAAGA+AGG 0.543662 4_1:+76748629 Msa0573530:CDS
GTGTGCAGAAGCAAATTCCC+TGG 0.546248 4_1:+76748596 Msa0573530:CDS
GCAGATATAACCAAAGAAAG+AGG 0.547218 4_1:-76748011 None:intergenic
TCATCTTCAAAGTCGTCAAA+GGG 0.547953 4_1:-76746725 None:intergenic
GAAAGGGGTGATGAGGTGCT+TGG 0.550156 4_1:-76746618 None:intergenic
GAAACCAGATTAACTAGAAG+TGG 0.554823 4_1:+76748882 Msa0573530:three_prime_UTR
AGATTAACTAGAAGTGGCGT+TGG 0.555583 4_1:+76748888 Msa0573530:three_prime_UTR
TTGCTCTTACTCTATGAAGC+TGG 0.556139 4_1:-76748796 None:intergenic
TTGTGTTACAGTTAGGAGTA+GGG 0.557446 4_1:+76748743 Msa0573530:three_prime_UTR
TCCCTGGAGTTAGATGGAGA+TGG 0.559224 4_1:+76748612 Msa0573530:CDS
AATTGGAGGTTAGGAACGCT+TGG 0.559456 4_1:+76747784 Msa0573530:CDS
GACGTTTCTCTGTTCATCCA+CGG 0.559987 4_1:+76748536 Msa0573530:CDS
ATATACAAATATATATGAAG+TGG 0.562035 4_1:-76748920 None:intergenic
TAGGCTTGACACACTTGAGA+TGG 0.566419 4_1:-76748052 None:intergenic
GTAAGTTTGGAAAATGGGAC+TGG 0.568508 4_1:+76748410 Msa0573530:CDS
TGAACAGAGAAACGTCTCCC+AGG 0.569762 4_1:-76748529 None:intergenic
ATCTCAAGTGTGTCAAGCCT+AGG 0.572917 4_1:+76748054 Msa0573530:CDS
TAGGAACGCTTGGTTAGCTG+TGG 0.580545 4_1:+76747794 Msa0573530:CDS
TATCTGGTGAACGTGATTTG+AGG 0.581565 4_1:-76746595 None:intergenic
ACGAGAGATGGAACAGATGA+GGG 0.582688 4_1:-76746570 None:intergenic
TGGTGACGGATTCGAGATTG+GGG 0.593691 4_1:-76746673 None:intergenic
GAATGAAATTGAAATCCGTA+AGG 0.594296 4_1:-76746763 None:intergenic
AACGAGAGATGGAACAGATG+AGG 0.597347 4_1:-76746571 None:intergenic
CCCATCTCCATCTAACTCCA+GGG 0.598276 4_1:-76748613 None:intergenic
AACTTAGATACCTTATGTTG+GGG 0.600732 4_1:-76748343 None:intergenic
AGACTGAAGTTTGACAAAGA+AGG 0.601585 4_1:-76748095 None:intergenic
ACTAACAAGTGGAAGAGATG+AGG 0.602954 4_1:-76746880 None:intergenic
AACAACACGCATCGTGATCG+TGG 0.606110 4_1:+76746400 Msa0573530:five_prime_UTR
TAAGTTTGGAAAATGGGACT+GGG 0.606420 4_1:+76748411 Msa0573530:CDS
GCTTCAAGGAAAAGCAACCT+AGG 0.609348 4_1:-76748071 None:intergenic
ATGAGATTTCCAGCAGCACA+AGG 0.623043 4_1:-76748569 None:intergenic
CTAGATCGGTTGAGTCGTTG+AGG 0.623467 4_1:-76746487 None:intergenic
AATCTCGAATCCGTCACCAT+CGG 0.623689 4_1:+76746677 Msa0573530:CDS
CAGAGAAACGTCTCCCAGGA+GGG 0.629157 4_1:-76748525 None:intergenic
CTTAGAATTTCTACAACCAG+CGG 0.636022 4_1:-76746461 None:intergenic
AGAGCTTGTTGCACATATCG+TGG 0.636306 4_1:+76748448 Msa0573530:CDS
GATGGAGAGTTCTTTCCGCA+GGG 0.636451 4_1:+76748388 Msa0573530:CDS
GTTTGTAAGATTGGATGATG+AGG 0.640974 4_1:+76747860 Msa0573530:CDS
GTTTAAGGAAAAGGGAGAGT+CGG 0.642281 4_1:+76748689 Msa0573530:three_prime_UTR
AAGAGAACGATCAACGCCGA+TGG 0.643423 4_1:-76746693 None:intergenic
ACAATGCCTTCAGCTTGAAG+CGG 0.645681 4_1:+76748166 Msa0573530:CDS
GATCGGTTGAGTCGTTGAGG+CGG 0.655845 4_1:-76746484 None:intergenic
GGTCAACGAACGAACTTCGG+AGG 0.658236 4_1:-76746549 None:intergenic
CCTGGAGTTAGATGGAGATG+GGG 0.661275 4_1:+76748614 Msa0573530:CDS
TTAATCGAATTGGAAAGCGA+AGG 0.664500 4_1:-76746797 None:intergenic
AATTTCTACAACCAGCGGAG+AGG 0.665838 4_1:-76746456 None:intergenic
ACAGAGAAACGTCTCCCAGG+AGG 0.695142 4_1:-76748526 None:intergenic
CACAAGGATCAGCATCACCG+TGG 0.702098 4_1:-76748553 None:intergenic
GGTGACGGATTCGAGATTGG+GGG 0.703117 4_1:-76746672 None:intergenic
TAACAAGTGGAAGAGATGAG+GGG 0.706106 4_1:-76746878 None:intergenic
AACACGCATCGTGATCGTGG+TGG 0.711865 4_1:+76746403 Msa0573530:five_prime_UTR
GTCTCTCTACAACAACACAA+TGG 0.730203 4_1:-76748226 None:intergenic
ATATGTTCAGAACAAGTGAG+CGG 0.731446 4_1:-76748967 None:intergenic
GAAAGAACAAGTGAACAACA+AGG 0.767673 4_1:-76749011 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATATTTTTAAAGATATTTT+AGG + chr4_1:76747231-76747250 Msa0573530:intron 10.0%
!!! TATCTTTAAAAATATCTACT+TGG - chr4_1:76747227-76747246 None:intergenic 15.0%
!! TATTGTAGATGATTAAAAAT+AGG - chr4_1:76747429-76747448 None:intergenic 15.0%
!! ATATACAAATATATATGAAG+TGG - chr4_1:76748923-76748942 None:intergenic 15.0%
!!! TCAATTTTTTCTGTCTTAAA+TGG - chr4_1:76746318-76746337 None:intergenic 20.0%
!! ATTTCAATTTCATTCAAGAT+TGG + chr4_1:76746771-76746790 Msa0573530:CDS 20.0%
!! AATGGACAATAAATAATCAA+AGG - chr4_1:76746926-76746945 None:intergenic 20.0%
!! GTATAAACTCAATTCTAAAT+TGG - chr4_1:76747048-76747067 None:intergenic 20.0%
!! TAATCATCTACAATAGTTAT+AGG + chr4_1:76747434-76747453 Msa0573530:intron 20.0%
!! AATCATCTACAATAGTTATA+GGG + chr4_1:76747435-76747454 Msa0573530:intron 20.0%
!! CATCAATCTATTATAACTAT+GGG - chr4_1:76747629-76747648 None:intergenic 20.0%
!!! AGATTTTGAATAAATTGTGT+TGG + chr4_1:76748772-76748791 Msa0573530:three_prime_UTR 20.0%
! ATTAGATTTGATAGGATGTA+GGG - chr4_1:76747200-76747219 None:intergenic 25.0%
!! CTCTATCATATCACATTTTT+AGG - chr4_1:76747263-76747282 None:intergenic 25.0%
! AAAATGTGATATGATAGAGT+CGG + chr4_1:76747264-76747283 Msa0573530:intron 25.0%
! CAGCTATAATAGATAATTGA+CGG + chr4_1:76747363-76747382 Msa0573530:intron 25.0%
! GCATCAATCTATTATAACTA+TGG - chr4_1:76747630-76747649 None:intergenic 25.0%
! GTAAAAAGACTTTACATTGA+TGG + chr4_1:76747651-76747670 Msa0573530:intron 25.0%
!!! ATCCTTTAGAGTTATATTTG+TGG + chr4_1:76747688-76747707 Msa0573530:intron 25.0%
! GATATGAAACTATTAGCAAA+AGG - chr4_1:76747736-76747755 None:intergenic 25.0%
! CAATAAGATTAAGTTCCTTA+AGG - chr4_1:76747917-76747936 None:intergenic 25.0%
! CTTAAGGAACTTAATCTTAT+TGG + chr4_1:76747915-76747934 Msa0573530:CDS 25.0%
! AAACTTAGATACCTTATGTT+GGG - chr4_1:76748347-76748366 None:intergenic 25.0%
! TAAACTTAGATACCTTATGT+TGG - chr4_1:76748348-76748367 None:intergenic 25.0%
!! AACATAAGGTATCTAAGTTT+AGG + chr4_1:76748347-76748366 Msa0573530:CDS 25.0%
!!! TGCATTTATTTTCTCTGTTT+TGG + chr4_1:76748493-76748512 Msa0573530:CDS 25.0%
! TCTGAACATATGTATATTCT+TGG + chr4_1:76748979-76748998 Msa0573530:three_prime_UTR 25.0%
!!! TCTTGAAAACGGTTTTGAAA+GGG - chr4_1:76746637-76746656 None:intergenic 30.0%
!!! TTCTTGAAAACGGTTTTGAA+AGG - chr4_1:76746638-76746657 None:intergenic 30.0%
GAATGAAATTGAAATCCGTA+AGG - chr4_1:76746766-76746785 None:intergenic 30.0%
TAGCATAATTGCAACTACAA+TGG - chr4_1:76746944-76746963 None:intergenic 30.0%
! ATTTCTAATTTTCTATGCCG+TGG + chr4_1:76747159-76747178 Msa0573530:intron 30.0%
GATTAGATTTGATAGGATGT+AGG - chr4_1:76747201-76747220 None:intergenic 30.0%
TTGGCATGATTAGATTTGAT+AGG - chr4_1:76747208-76747227 None:intergenic 30.0%
! CAACAGTGTAAAACCTTTTT+AGG - chr4_1:76747307-76747326 None:intergenic 30.0%
! CTAAAAAGGTTTTACACTGT+TGG + chr4_1:76747305-76747324 Msa0573530:intron 30.0%
! GCTGCTAGATGATTTAAAAT+AGG - chr4_1:76747347-76747366 None:intergenic 30.0%
AGATAATTGACGGTGTAAAA+AGG + chr4_1:76747373-76747392 Msa0573530:intron 30.0%
GTTTGTGTAATAAAGTGCAT+TGG - chr4_1:76747544-76747563 None:intergenic 30.0%
TACAAACTCAAGAGTCAAAT+TGG - chr4_1:76747847-76747866 None:intergenic 30.0%
GATTAAGTTCCTTAAGGTTA+GGG - chr4_1:76747911-76747930 None:intergenic 30.0%
AGATTAAGTTCCTTAAGGTT+AGG - chr4_1:76747912-76747931 None:intergenic 30.0%
GAACTTAATCTTATTGGTGT+TGG + chr4_1:76747921-76747940 Msa0573530:CDS 30.0%
TGAATTATTTCCCCAACATA+AGG + chr4_1:76748333-76748352 Msa0573530:CDS 30.0%
AACTTAGATACCTTATGTTG+GGG - chr4_1:76748346-76748365 None:intergenic 30.0%
! GTATCTAAGTTTAGGTTATG+AGG + chr4_1:76748355-76748374 Msa0573530:CDS 30.0%
CAAGAGTAAACTCAATTTCA+AGG - chr4_1:76748475-76748494 None:intergenic 30.0%
AATATGGAAAGAAGGAATCA+AGG + chr4_1:76748637-76748656 Msa0573530:CDS 30.0%
ATCTAGTGTGTTTAAGGAAA+AGG + chr4_1:76748680-76748699 Msa0573530:exon 30.0%
TCTAGTGTGTTTAAGGAAAA+GGG + chr4_1:76748681-76748700 Msa0573530:exon 30.0%
TTACAGATTGTGTTACAGTT+AGG + chr4_1:76748736-76748755 Msa0573530:three_prime_UTR 30.0%
AGTTTGAACTACACTTGAAA+AGG - chr4_1:76748860-76748879 None:intergenic 30.0%
! TTTCAAGTGTAGTTCAAACT+AGG + chr4_1:76748860-76748879 Msa0573530:three_prime_UTR 30.0%
GGTGTTAGAAATTCCTTCAA+TGG + chr4_1:76746424-76746443 Msa0573530:exon 35.0%
CTTAGAATTTCTACAACCAG+CGG - chr4_1:76746464-76746483 None:intergenic 35.0%
!!! CTTGAAAACGGTTTTGAAAG+GGG - chr4_1:76746636-76746655 None:intergenic 35.0%
TCATCTTCAAAGTCGTCAAA+GGG - chr4_1:76746728-76746747 None:intergenic 35.0%
TTCATCTTCAAAGTCGTCAA+AGG - chr4_1:76746729-76746748 None:intergenic 35.0%
ATCTGATGATCTTCACCTTA+CGG + chr4_1:76746748-76746767 Msa0573530:CDS 35.0%
TTAATCGAATTGGAAAGCGA+AGG - chr4_1:76746800-76746819 None:intergenic 35.0%
! ATTTCTGGGTTCAATCTTGT+TGG + chr4_1:76746837-76746856 Msa0573530:CDS 35.0%
ATAAGAAGCTGACTAACAAG+TGG - chr4_1:76746894-76746913 None:intergenic 35.0%
TCAAATGTTTCAGAAGTCTC+TGG - chr4_1:76746992-76747011 None:intergenic 35.0%
!! GGTAAAGTTTTATACCTCCA+CGG - chr4_1:76747179-76747198 None:intergenic 35.0%
ATATGATAGAGTCGGTTGAT+CGG + chr4_1:76747272-76747291 Msa0573530:intron 35.0%
CGCCACAAATATAACTCTAA+AGG - chr4_1:76747693-76747712 None:intergenic 35.0%
TAGAGTTATATTTGTGGCGT+TGG + chr4_1:76747694-76747713 Msa0573530:intron 35.0%
TTTCATATCCTTTGTGCTAC+AGG + chr4_1:76747747-76747766 Msa0573530:intron 35.0%
GACTCTTGAGTTTGTAAGAT+TGG + chr4_1:76747851-76747870 Msa0573530:CDS 35.0%
GTTTGTAAGATTGGATGATG+AGG + chr4_1:76747860-76747879 Msa0573530:CDS 35.0%
TACTTGTTTCCCTAACCTTA+AGG + chr4_1:76747899-76747918 Msa0573530:CDS 35.0%
!! CTTAATCTTATTGGTGTTGG+AGG + chr4_1:76747924-76747943 Msa0573530:CDS 35.0%
AGGTGTGAGAGATTAATCTT+AGG - chr4_1:76747960-76747979 None:intergenic 35.0%
TTCAAATGCTCCTCTTTCTT+TGG + chr4_1:76748001-76748020 Msa0573530:CDS 35.0%
GCAGATATAACCAAAGAAAG+AGG - chr4_1:76748014-76748033 None:intergenic 35.0%
AGATGGAAATCAACAAGACT+TGG - chr4_1:76748038-76748057 None:intergenic 35.0%
AGACTGAAGTTTGACAAAGA+AGG - chr4_1:76748098-76748117 None:intergenic 35.0%
! GAGCTAGTGTTGAAAAATTG+TGG + chr4_1:76748137-76748156 Msa0573530:CDS 35.0%
! TAAGTTTAGGTTATGAGGCT+TGG + chr4_1:76748360-76748379 Msa0573530:CDS 35.0%
TAAGTTTGGAAAATGGGACT+GGG + chr4_1:76748411-76748430 Msa0573530:CDS 35.0%
AGAAGGAATCAAGGATACTT+TGG + chr4_1:76748646-76748665 Msa0573530:CDS 35.0%
! AAGGATACTTTGGTTTCAGA+TGG + chr4_1:76748656-76748675 Msa0573530:CDS 35.0%
ATTGTGTTACAGTTAGGAGT+AGG + chr4_1:76748742-76748761 Msa0573530:three_prime_UTR 35.0%
TTGTGTTACAGTTAGGAGTA+GGG + chr4_1:76748743-76748762 Msa0573530:three_prime_UTR 35.0%
GAAACCAGATTAACTAGAAG+TGG + chr4_1:76748882-76748901 Msa0573530:three_prime_UTR 35.0%
ATATGTTCAGAACAAGTGAG+CGG - chr4_1:76748970-76748989 None:intergenic 35.0%
GAAAGAACAAGTGAACAACA+AGG - chr4_1:76749014-76749033 None:intergenic 35.0%
! TGATTTGAGGTAACGAGAGA+TGG - chr4_1:76746585-76746604 None:intergenic 40.0%
TATCTGGTGAACGTGATTTG+AGG - chr4_1:76746598-76746617 None:intergenic 40.0%
GAGAAGGAGGTTCTTGAAAA+CGG - chr4_1:76746648-76746667 None:intergenic 40.0%
AGAGAGCGACTTAATCGAAT+TGG - chr4_1:76746810-76746829 None:intergenic 40.0%
!! GCTCTCTGTTTCTGATTTCT+GGG + chr4_1:76746823-76746842 Msa0573530:CDS 40.0%
TAACAAGTGGAAGAGATGAG+GGG - chr4_1:76746881-76746900 None:intergenic 40.0%
CTAACAAGTGGAAGAGATGA+GGG - chr4_1:76746882-76746901 None:intergenic 40.0%
ACTAACAAGTGGAAGAGATG+AGG - chr4_1:76746883-76746902 None:intergenic 40.0%
GAATTGAATTGTGACCCCAA+TGG + chr4_1:76747010-76747029 Msa0573530:intron 40.0%
! TCTAATTTTCTATGCCGTGG+AGG + chr4_1:76747162-76747181 Msa0573530:intron 40.0%
TCGGATGCATATGCCTAAAA+AGG + chr4_1:76747291-76747310 Msa0573530:intron 40.0%
!! AGAAAGTTTAAAGAGTGCCC+CGG - chr4_1:76747478-76747497 None:intergenic 40.0%
CACACCTTCAAACTTGTCAA+TGG + chr4_1:76747973-76747992 Msa0573530:CDS 40.0%
CCTTCAAACTTGTCAATGGT+CGG + chr4_1:76747977-76747996 Msa0573530:CDS 40.0%
!! TTGACAAAGAAGGTGCTTCA+AGG - chr4_1:76748088-76748107 None:intergenic 40.0%
GTCTCTCTACAACAACACAA+TGG - chr4_1:76748229-76748248 None:intergenic 40.0%
AAGAACAGAGAGAGTTGTAG+AGG + chr4_1:76748268-76748287 Msa0573530:CDS 40.0%
GTTGATCAAGTAGGAGAGTT+TGG + chr4_1:76748296-76748315 Msa0573530:CDS 40.0%
! CATTTTCCAAACTTACCCTG+CGG - chr4_1:76748406-76748425 None:intergenic 40.0%
GCAGGGTAAGTTTGGAAAAT+GGG + chr4_1:76748405-76748424 Msa0573530:CDS 40.0%
GTAAGTTTGGAAAATGGGAC+TGG + chr4_1:76748410-76748429 Msa0573530:CDS 40.0%
TTAGATGGAGATGGGGAATA+TGG + chr4_1:76748621-76748640 Msa0573530:CDS 40.0%
AGATGGGGAATATGGAAAGA+AGG + chr4_1:76748629-76748648 Msa0573530:CDS 40.0%
GATGGCATCTAGTGTGTTTA+AGG + chr4_1:76748674-76748693 Msa0573530:exon 40.0%
GTTTAAGGAAAAGGGAGAGT+CGG + chr4_1:76748689-76748708 Msa0573530:three_prime_UTR 40.0%
GTCGGTCTTTGAATTGTTGT+AGG + chr4_1:76748707-76748726 Msa0573530:three_prime_UTR 40.0%
TTGCTCTTACTCTATGAAGC+TGG - chr4_1:76748799-76748818 None:intergenic 40.0%
! AACGCCACTTCTAGTTAATC+TGG - chr4_1:76748889-76748908 None:intergenic 40.0%
AGATTAACTAGAAGTGGCGT+TGG + chr4_1:76748888-76748907 Msa0573530:three_prime_UTR 40.0%
! TTGTTTGCGCATGTTTTGCG+TGG - chr4_1:76746385-76746404 None:intergenic 45.0%
GAGGGTAGATTCTCCATTGA+AGG - chr4_1:76746440-76746459 None:intergenic 45.0%
ATTTCTACAACCAGCGGAGA+GGG - chr4_1:76746458-76746477 None:intergenic 45.0%
AATTTCTACAACCAGCGGAG+AGG - chr4_1:76746459-76746478 None:intergenic 45.0%
GAGTCTGCATCTTGCTAGAT+CGG - chr4_1:76746504-76746523 None:intergenic 45.0%
ACGAGAGATGGAACAGATGA+GGG - chr4_1:76746573-76746592 None:intergenic 45.0%
AACGAGAGATGGAACAGATG+AGG - chr4_1:76746574-76746593 None:intergenic 45.0%
!! ATGGTGACGGATTCGAGATT+GGG - chr4_1:76746677-76746696 None:intergenic 45.0%
AATCTCGAATCCGTCACCAT+CGG + chr4_1:76746677-76746696 Msa0573530:CDS 45.0%
!! CGCTCTCTGTTTCTGATTTC+TGG + chr4_1:76746822-76746841 Msa0573530:CDS 45.0%
TGGTTTCTGCAACTGCCATT+GGG - chr4_1:76747028-76747047 None:intergenic 45.0%
TTGGTTTCTGCAACTGCCAT+TGG - chr4_1:76747029-76747048 None:intergenic 45.0%
!! AAAGTTTAAAGAGTGCCCCG+GGG - chr4_1:76747476-76747495 None:intergenic 45.0%
!! GAAAGTTTAAAGAGTGCCCC+GGG - chr4_1:76747477-76747496 None:intergenic 45.0%
AAATGTTAACGAGTGCCCCT+GGG - chr4_1:76747606-76747625 None:intergenic 45.0%
ATCCTTTGTGCTACAGGCTA+TGG + chr4_1:76747753-76747772 Msa0573530:intron 45.0%
AACCATAGCCTGTAGCACAA+AGG - chr4_1:76747758-76747777 None:intergenic 45.0%
TTGTGCTACAGGCTATGGTT+TGG + chr4_1:76747758-76747777 Msa0573530:intron 45.0%
!! AGGCTATGGTTTGGTGCAAT+TGG + chr4_1:76747767-76747786 Msa0573530:intron 45.0%
!! CTATGGTTTGGTGCAATTGG+AGG + chr4_1:76747770-76747789 Msa0573530:CDS 45.0%
!! GTTTGGTGCAATTGGAGGTT+AGG + chr4_1:76747775-76747794 Msa0573530:CDS 45.0%
AATTGGAGGTTAGGAACGCT+TGG + chr4_1:76747784-76747803 Msa0573530:CDS 45.0%
CCGACCATTGACAAGTTTGA+AGG - chr4_1:76747980-76747999 None:intergenic 45.0%
TAGGCTTGACACACTTGAGA+TGG - chr4_1:76748055-76748074 None:intergenic 45.0%
ATCTCAAGTGTGTCAAGCCT+AGG + chr4_1:76748054-76748073 Msa0573530:CDS 45.0%
GCTTCAAGGAAAAGCAACCT+AGG - chr4_1:76748074-76748093 None:intergenic 45.0%
ACAATGCCTTCAGCTTGAAG+CGG + chr4_1:76748166-76748185 Msa0573530:CDS 45.0%
ACAGAGAGAGTTGTAGAGGT+TGG + chr4_1:76748272-76748291 Msa0573530:CDS 45.0%
! GAGGTTGGAGTTGATCAAGT+AGG + chr4_1:76748287-76748306 Msa0573530:CDS 45.0%
!! TTATGAGGCTTGGCTTGTGA+TGG + chr4_1:76748370-76748389 Msa0573530:CDS 45.0%
TTCTTTCCGCAGGGTAAGTT+TGG + chr4_1:76748397-76748416 Msa0573530:CDS 45.0%
CGCAGGGTAAGTTTGGAAAA+TGG + chr4_1:76748404-76748423 Msa0573530:CDS 45.0%
!! AGAGCTTGTTGCACATATCG+TGG + chr4_1:76748448-76748467 Msa0573530:CDS 45.0%
TTTGGATAAATGCACCCTCC+TGG + chr4_1:76748511-76748530 Msa0573530:CDS 45.0%
TTGGATAAATGCACCCTCCT+GGG + chr4_1:76748512-76748531 Msa0573530:CDS 45.0%
GACGTTTCTCTGTTCATCCA+CGG + chr4_1:76748536-76748555 Msa0573530:CDS 45.0%
ATGAGATTTCCAGCAGCACA+AGG - chr4_1:76748572-76748591 None:intergenic 45.0%
GCAAATTCCCTGGAGTTAGA+TGG + chr4_1:76748606-76748625 Msa0573530:CDS 45.0%
AACAACACGCATCGTGATCG+TGG + chr4_1:76746400-76746419 Msa0573530:five_prime_UTR 50.0%
CTAGATCGGTTGAGTCGTTG+AGG - chr4_1:76746490-76746509 None:intergenic 50.0%
GAGGGTCAACGAACGAACTT+CGG - chr4_1:76746555-76746574 None:intergenic 50.0%
! GTGATGAGGTGCTTGGTATC+TGG - chr4_1:76746614-76746633 None:intergenic 50.0%
!!! CGGTTTTGAAAGGGGTGATG+AGG - chr4_1:76746628-76746647 None:intergenic 50.0%
!! TGGTGACGGATTCGAGATTG+GGG - chr4_1:76746676-76746695 None:intergenic 50.0%
!! GATGGTGACGGATTCGAGAT+TGG - chr4_1:76746678-76746697 None:intergenic 50.0%
AAGAGAACGATCAACGCCGA+TGG - chr4_1:76746696-76746715 None:intergenic 50.0%
GGTTTCTGCAACTGCCATTG+GGG - chr4_1:76747027-76747046 None:intergenic 50.0%
AAGTGCTTAACCAGTGTCCC+AGG + chr4_1:76747586-76747605 Msa0573530:intron 50.0%
AGTGCTTAACCAGTGTCCCA+GGG + chr4_1:76747587-76747606 Msa0573530:intron 50.0%
GAAATGTTAACGAGTGCCCC+TGG - chr4_1:76747607-76747626 None:intergenic 50.0%
! TAGGAACGCTTGGTTAGCTG+TGG + chr4_1:76747794-76747813 Msa0573530:CDS 50.0%
! AACGCTTGGTTAGCTGTGGA+TGG + chr4_1:76747798-76747817 Msa0573530:CDS 50.0%
TGATGGAGAGTTCTTTCCGC+AGG + chr4_1:76748387-76748406 Msa0573530:CDS 50.0%
GATGGAGAGTTCTTTCCGCA+GGG + chr4_1:76748388-76748407 Msa0573530:CDS 50.0%
TGAACAGAGAAACGTCTCCC+AGG - chr4_1:76748532-76748551 None:intergenic 50.0%
GTGTGCAGAAGCAAATTCCC+TGG + chr4_1:76748596-76748615 Msa0573530:CDS 50.0%
TCCCTGGAGTTAGATGGAGA+TGG + chr4_1:76748612-76748631 Msa0573530:CDS 50.0%
CCCATCTCCATCTAACTCCA+GGG - chr4_1:76748616-76748635 None:intergenic 50.0%
CCCTGGAGTTAGATGGAGAT+GGG + chr4_1:76748613-76748632 Msa0573530:CDS 50.0%
CCTGGAGTTAGATGGAGATG+GGG + chr4_1:76748614-76748633 Msa0573530:CDS 50.0%
AACACGCATCGTGATCGTGG+TGG + chr4_1:76746403-76746422 Msa0573530:five_prime_UTR 55.0%
GATCGGTTGAGTCGTTGAGG+CGG - chr4_1:76746487-76746506 None:intergenic 55.0%
TTCGGAGGAAGCGGAGTTGA+GGG - chr4_1:76746537-76746556 None:intergenic 55.0%
CGAACGAACTTCGGAGGAAG+CGG - chr4_1:76746546-76746565 None:intergenic 55.0%
GGTCAACGAACGAACTTCGG+AGG - chr4_1:76746552-76746571 None:intergenic 55.0%
! GAAAGGGGTGATGAGGTGCT+TGG - chr4_1:76746621-76746640 None:intergenic 55.0%
CGGATTCGAGATTGGGGGAT+CGG - chr4_1:76746670-76746689 None:intergenic 55.0%
!! GGTGACGGATTCGAGATTGG+GGG - chr4_1:76746675-76746694 None:intergenic 55.0%
ACGATCAACGCCGATGGTGA+CGG - chr4_1:76746690-76746709 None:intergenic 55.0%
!! GGTCTTGTTAACGAGTGCCC+CGG + chr4_1:76747456-76747475 Msa0573530:intron 55.0%
! GTCTTGTTAACGAGTGCCCC+GGG + chr4_1:76747457-76747476 Msa0573530:intron 55.0%
TCTTGTTAACGAGTGCCCCG+GGG + chr4_1:76747458-76747477 Msa0573530:intron 55.0%
GTGCTTAACCAGTGTCCCAG+GGG + chr4_1:76747588-76747607 Msa0573530:intron 55.0%
CGCATTCCGCTTCAAGCTGA+AGG - chr4_1:76748175-76748194 None:intergenic 55.0%
CAGAGAAACGTCTCCCAGGA+GGG - chr4_1:76748528-76748547 None:intergenic 55.0%
ACAGAGAAACGTCTCCCAGG+AGG - chr4_1:76748529-76748548 None:intergenic 55.0%
CACAAGGATCAGCATCACCG+TGG - chr4_1:76748556-76748575 None:intergenic 55.0%
GATGCTGATCCTTGTGCTGC+TGG + chr4_1:76748560-76748579 Msa0573530:CDS 55.0%
CCCCATCTCCATCTAACTCC+AGG - chr4_1:76748617-76748636 None:intergenic 55.0%
GGAGAATCTACCCTCTCCGC+TGG + chr4_1:76746445-76746464 Msa0573530:CDS 60.0%
CTTCGGAGGAAGCGGAGTTG+AGG - chr4_1:76746538-76746557 None:intergenic 60.0%
GATTGGGGGATCGGAGAAGG+AGG - chr4_1:76746661-76746680 None:intergenic 60.0%
CGAGATTGGGGGATCGGAGA+AGG - chr4_1:76746664-76746683 None:intergenic 60.0%
AACGAGTGCCCCTGGGACAC+TGG - chr4_1:76747599-76747618 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 76746309 76749081 76746309 ID=Msa0573530;Name=Msa0573530
chr4_1 mRNA 76746309 76749081 76746309 ID=Msa0573530-mRNA-1;Parent=Msa0573530;Name=Msa0573530-mRNA-1;_AED=0.00;_eAED=0.00;_QI=134|1|1|1|1|1|2|396|456
chr4_1 exon 76746309 76746896 76746309 ID=Msa0573530-mRNA-1:exon:26871;Parent=Msa0573530-mRNA-1
chr4_1 exon 76747769 76749081 76747769 ID=Msa0573530-mRNA-1:exon:26872;Parent=Msa0573530-mRNA-1
chr4_1 five_prime_UTR 76746309 76746442 76746309 ID=Msa0573530-mRNA-1:five_prime_utr;Parent=Msa0573530-mRNA-1
chr4_1 CDS 76746443 76746896 76746443 ID=Msa0573530-mRNA-1:cds;Parent=Msa0573530-mRNA-1
chr4_1 CDS 76747769 76748685 76747769 ID=Msa0573530-mRNA-1:cds;Parent=Msa0573530-mRNA-1
chr4_1 three_prime_UTR 76748686 76749081 76748686 ID=Msa0573530-mRNA-1:three_prime_utr;Parent=Msa0573530-mRNA-1
Gene Sequence

>Msa0573530

ATGGAGAATCTACCCTCTCCGCTGGTTGTAGAAATTCTAAGCCGCCTCAACGACTCAACCGATCTAGCAAGATGCAGACTCGTTTCAAACACCCTCAACTCCGCTTCCTCCGAAGTTCGTTCGTTGACCCTCATCTGTTCCATCTCTCGTTACCTCAAATCACGTTCACCAGATACCAAGCACCTCATCACCCCTTTCAAAACCGTTTTCAAGAACCTCCTTCTCCGATCCCCCAATCTCGAATCCGTCACCATCGGCGTTGATCGTTCTCTTGTTGAGATGCCCTTTGACGACTTTGAAGATGAATCTGATGATCTTCACCTTACGGATTTCAATTTCATTCAAGATTGGCTTCCTTCGCTTTCCAATTCGATTAAGTCGCTCTCTGTTTCTGATTTCTGGGTTCAATCTTGTTGGAGACGTTCACGAGCTTTGCCCCTCATCTCTTCCACTTGCTATGGTTTGGTGCAATTGGAGGTTAGGAACGCTTGGTTAGCTGTGGATGGTTTGTGTTTGATGCACACATTGACCAATTTGACTCTTGAGTTTGTAAGATTGGATGATGAGGATTTGAACATGATCAATACTTGTTTCCCTAACCTTAAGGAACTTAATCTTATTGGTGTTGGAGGACTTGAAGAGCCTAAGATTAATCTCTCACACCTTCAAACTTGTCAATGGTCGGTTTCAAATGCTCCTCTTTCTTTGGTTATATCTGCTCCAAGTCTTGTTGATTTCCATCTCAAGTGTGTCAAGCCTAGGTTGCTTTTCCTTGAAGCACCTTCTTTGTCAAACTTCAGTCTTTCTCTTGAGAACACTGATGAGCTAGTGTTGAAAAATTGTGGCAACATACAATGCCTTCAGCTTGAAGCGGAATGCGTCTCTCTTGCTTACATTTTAAGCATGTTTCGCCATTGTGTTGTTGTAGAGAGACTGAGCTTAGATTTTGTGAGAAGAACAGAGAGAGTTGTAGAGGTTGGAGTTGATCAAGTAGGAGAGTTTGGTCTTGATACGTTGTTGAATTATTTCCCCAACATAAGGTATCTAAGTTTAGGTTATGAGGCTTGGCTTGTGATGGAGAGTTCTTTCCGCAGGGTAAGTTTGGAAAATGGGACTGGGATGAAGTCTTTGAAAGAGCTTGTTGCACATATCGTGGTCCTTGAAATTGAGTTTACTCTTGCATTTATTTTCTCTGTTTTGGATAAATGCACCCTCCTGGGAGACGTTTCTCTGTTCATCCACGGTGATGCTGATCCTTGTGCTGCTGGAAATCTCATCTCTGTGTGCAGAAGCAAATTCCCTGGAGTTAGATGGAGATGGGGAATATGGAAAGAAGGAATCAAGGATACTTTGGTTTCAGATGGCATCTAG
Protein sequence

>Msa0573530

MENLPSPLVVEILSRLNDSTDLARCRLVSNTLNSASSEVRSLTLICSISRYLKSRSPDTKHLITPFKTVFKNLLLRSPNLESVTIGVDRSLVEMPFDDFEDESDDLHLTDFNFIQDWLPSLSNSIKSLSVSDFWVQSCWRRSRALPLISSTCYGLVQLEVRNAWLAVDGLCLMHTLTNLTLEFVRLDDEDLNMINTCFPNLKELNLIGVGGLEEPKINLSHLQTCQWSVSNAPLSLVISAPSLVDFHLKCVKPRLLFLEAPSLSNFSLSLENTDELVLKNCGNIQCLQLEAECVSLAYILSMFRHCVVVERLSLDFVRRTERVVEVGVDQVGEFGLDTLLNYFPNIRYLSLGYEAWLVMESSFRRVSLENGTGMKSLKELVAHIVVLEIEFTLAFIFSVLDKCTLLGDVSLFIHGDADPCAAGNLISVCRSKFPGVRWRWGIWKEGIKDTLVSDGI*