AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0573360

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0573360 MtrunA17_Chr8g0389391 93.796 274 17 0 48 321 1 274 1.80e-176 495
Msa0573360 MtrunA17_Chr8g0389401 88.776 98 11 0 359 456 1 98 1.03e-55 180
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0573360 AT4G29420.1 45.952 457 231 6 1 452 1 446 3.76e-134 394

Find 134 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCTCTCTGTTTCTGATTTC+TGG -0.102421 4_1:+76545659 Msa0573360:CDS
CTTAAGGAACTTAATCTTAT+TGG 0.148001 4_1:+76546723 Msa0573360:CDS
TCTGAACATATGTATATTCT+TGG 0.209320 4_1:+76547791 Msa0573360:three_prime_UTR
GATGGCATCTAGTGTGTTTA+AGG 0.212657 4_1:+76547482 Msa0573360:exon
CGCAGGGTAAGTTTGGAAAA+TGG 0.222783 4_1:+76547212 Msa0573360:CDS
TTCAAATGCTCCTCTTTCTT+TGG 0.250922 4_1:+76546809 Msa0573360:CDS
TTAGATGGAGATGGGGAATA+TGG 0.260868 4_1:+76547429 Msa0573360:CDS
TTCTTTCCGCAGGGTAAGTT+TGG 0.275948 4_1:+76547205 Msa0573360:CDS
ATTTCTGGGTTCAATCTTGT+TGG 0.315218 4_1:+76545674 Msa0573360:CDS
GCTCTCTGTTTCTGATTTCT+GGG 0.347427 4_1:+76545660 Msa0573360:CDS
AACATAAGGTATCTAAGTTT+AGG 0.356561 4_1:+76547155 Msa0573360:CDS
AGGCTATGGTTTGGTGCAAT+TGG 0.363155 4_1:+76546575 Msa0573360:intron
AGATTAAGTTCCTTAAGGTT+AGG 0.364458 4_1:-76546717 None:intergenic
ACAGAGAGAGTTGTAGAGGT+TGG 0.367243 4_1:+76547080 Msa0573360:CDS
TACAAACTCAAGAGTCAAAT+TGG 0.367246 4_1:-76546652 None:intergenic
TACTTGTTTCCCTAACCTTA+AGG 0.368816 4_1:+76546707 Msa0573360:CDS
AACGCCACTTCTAGTTAATC+TGG 0.369392 4_1:-76547698 None:intergenic
CAATAAGATTAAGTTCCTTA+AGG 0.377361 4_1:-76546722 None:intergenic
AGAGAGCGACTTAATCGAAT+TGG 0.381163 4_1:-76545644 None:intergenic
AAACTTAGATACCTTATGTT+GGG 0.381371 4_1:-76547152 None:intergenic
GTTTGGTGCAATTGGAGGTT+AGG 0.383923 4_1:+76546583 Msa0573360:CDS
ATTTCAATTTCATTCAAGAT+TGG 0.385697 4_1:+76545608 Msa0573360:CDS
GACTCTTGAGTTTGTAAGAT+TGG 0.385958 4_1:+76546659 Msa0573360:CDS
GTTGATCAAGTAGGAGAGTT+TGG 0.393214 4_1:+76547104 Msa0573360:CDS
GTGATGAGGTGCTTGGTATC+TGG 0.398273 4_1:-76545448 None:intergenic
GATTAAGTTCCTTAAGGTTA+GGG 0.398337 4_1:-76546716 None:intergenic
GATGCTGATCCTTGTGCTGC+TGG 0.400589 4_1:+76547368 Msa0573360:CDS
ATGGTGACGGATTCAAGATT+GGG 0.404295 4_1:-76545511 None:intergenic
ATCTAGTGTGTTTAAGGAAA+AGG 0.413335 4_1:+76547488 Msa0573360:exon
GTCGGTCTTTGAATTGTTGT+AGG 0.416315 4_1:+76547515 Msa0573360:three_prime_UTR
AGAAGGAATCAAGGATACTT+TGG 0.419448 4_1:+76547454 Msa0573360:CDS
GCAAATTCCCTGGAGTTAGA+TGG 0.420453 4_1:+76547414 Msa0573360:CDS
TGAATTATTTCCCCAACATA+AGG 0.424575 4_1:+76547141 Msa0573360:CDS
GGTGTTAGAAATTCCTTCAA+TGG 0.424696 4_1:+76545261 Msa0573360:exon
AGGTGTGAGAGATTAATCTT+AGG 0.425607 4_1:-76546765 None:intergenic
GTATCTAAGTTTAGGTTATG+AGG 0.429251 4_1:+76547163 Msa0573360:CDS
CCGACCATTGACAAGTTTGA+AGG 0.432550 4_1:-76546785 None:intergenic
TTACAGATTGTGTTACAGTT+AGG 0.435675 4_1:+76547544 Msa0573360:three_prime_UTR
GCAGGGTAAGTTTGGAAAAT+GGG 0.438676 4_1:+76547213 Msa0573360:CDS
GATGGTGACGGATTCAAGAT+TGG 0.439228 4_1:-76545512 None:intergenic
TTTGGATAAATGCACCCTCC+TGG 0.440415 4_1:+76547319 Msa0573360:CDS
TAAGTTTAGGTTATGAGGCT+TGG 0.443271 4_1:+76547168 Msa0573360:CDS
GAACTTAATCTTATTGGTGT+TGG 0.446999 4_1:+76546729 Msa0573360:CDS
CCCCATCTCCATCTAACTCC+AGG 0.448635 4_1:-76547422 None:intergenic
CTTAATCTTATTGGTGTTGG+AGG 0.450290 4_1:+76546732 Msa0573360:CDS
TCGTAAGTGTAGTTCAAACT+AGG 0.452991 4_1:+76547672 Msa0573360:three_prime_UTR
CAAGAGTAAACTCAATTTCA+AGG 0.453685 4_1:-76547280 None:intergenic
ATCTGATGATCTTCACCTTA+CGG 0.465937 4_1:+76545585 Msa0573360:CDS
TTGACAAAGAAGGTGCTTCA+AGG 0.471175 4_1:-76546893 None:intergenic
TTTGAACTACACTTACGAAA+AGG 0.480585 4_1:-76547667 None:intergenic
GAGGTTGGAGTTGATCAAGT+AGG 0.483396 4_1:+76547095 Msa0573360:CDS
TTGTGCTACAGGCTATGGTT+TGG 0.489604 4_1:+76546566 Msa0573360:intron
CCCTGGAGTTAGATGGAGAT+GGG 0.491028 4_1:+76547421 Msa0573360:CDS
CTATGGTTTGGTGCAATTGG+AGG 0.491781 4_1:+76546578 Msa0573360:CDS
TTCGGAGGAAGCGGAGTTGA+GGG 0.493295 4_1:-76545371 None:intergenic
TCTAGTGTGTTTAAGGAAAA+GGG 0.495016 4_1:+76547489 Msa0573360:exon
AAGAACAGAGAGAGTTGTAG+AGG 0.501443 4_1:+76547076 Msa0573360:CDS
GAGAAGGAGGTTCTTGAAAA+CGG 0.503343 4_1:-76545482 None:intergenic
TGATGGAGAGTTCTTTCCGC+AGG 0.504774 4_1:+76547195 Msa0573360:CDS
GAGGGTAGATTCTCCATTGA+AGG 0.510419 4_1:-76545274 None:intergenic
CGCATTCCGCTTCAAGCTGA+AGG 0.511737 4_1:-76546980 None:intergenic
AAGGATACTTTGGTTTCAGA+TGG 0.512935 4_1:+76547464 Msa0573360:CDS
TAAACTTAGATACCTTATGT+TGG 0.515466 4_1:-76547153 None:intergenic
CTTCGGAGGAAGCGGAGTTG+AGG 0.516753 4_1:-76545372 None:intergenic
TTGGATAAATGCACCCTCCT+GGG 0.518672 4_1:+76547320 Msa0573360:CDS
AACGCTTGGTTAGCTGTGGA+TGG 0.519585 4_1:+76546606 Msa0573360:CDS
CGAACGAACTTCGGAGGAAG+CGG 0.523808 4_1:-76545380 None:intergenic
AATATGGAAAGAAGGAATCA+AGG 0.525029 4_1:+76547445 Msa0573360:CDS
CACACCTTCAAACTTGTCAA+TGG 0.525428 4_1:+76546781 Msa0573360:CDS
ATTTCTACAACCAGCGGAGA+GGG 0.527269 4_1:-76545292 None:intergenic
TTCATCTTCAAAGTCGTCAA+AGG 0.528968 4_1:-76545563 None:intergenic
AGATGGAAATCAACAAGACT+TGG 0.529529 4_1:-76546843 None:intergenic
GAGGGTCAACGAACGAACTT+CGG 0.530729 4_1:-76545389 None:intergenic
ACGATCAACGCCGATGGTGA+CGG 0.532340 4_1:-76545524 None:intergenic
CCTTCAAACTTGTCAATGGT+CGG 0.533893 4_1:+76546785 Msa0573360:CDS
TGATTTGAGGTAACGAGAGA+TGG 0.534176 4_1:-76545419 None:intergenic
ATTGTGTTACAGTTAGGAGT+AGG 0.538559 4_1:+76547550 Msa0573360:three_prime_UTR
CTAACAAGTGGAAGAGATGA+GGG 0.540516 4_1:-76545716 None:intergenic
GAGTCTGCATCTTGCTAGAT+CGG 0.541109 4_1:-76545338 None:intergenic
TTATGAGGCTTGGCTTGTGA+TGG 0.541430 4_1:+76547178 Msa0573360:CDS
GGAGAATCTACCCTCTCCGC+TGG 0.541471 4_1:+76545282 Msa0573360:CDS
AGATGGGGAATATGGAAAGA+AGG 0.543662 4_1:+76547437 Msa0573360:CDS
GTGTGCAGAAGCAAATTCCC+TGG 0.546248 4_1:+76547404 Msa0573360:CDS
GCAGATATAACCAAAGAAAG+AGG 0.547218 4_1:-76546819 None:intergenic
TCATCTTCAAAGTCGTCAAA+GGG 0.547953 4_1:-76545562 None:intergenic
GAAAGGGGTGATGAGGTGCT+TGG 0.550156 4_1:-76545455 None:intergenic
GAAACCAGATTAACTAGAAG+TGG 0.554823 4_1:+76547694 Msa0573360:three_prime_UTR
AGATTAACTAGAAGTGGCGT+TGG 0.555583 4_1:+76547700 Msa0573360:three_prime_UTR
TTGTGTTACAGTTAGGAGTA+GGG 0.557446 4_1:+76547551 Msa0573360:three_prime_UTR
TCCCTGGAGTTAGATGGAGA+TGG 0.559224 4_1:+76547420 Msa0573360:CDS
AATTGGAGGTTAGGAACGCT+TGG 0.559456 4_1:+76546592 Msa0573360:CDS
GACGTTTCTCTGTTCATCCA+CGG 0.559987 4_1:+76547344 Msa0573360:CDS
ATATACAAATATATATGAAG+TGG 0.562035 4_1:-76547732 None:intergenic
TAGGCTTGACACACTTGAGA+TGG 0.566419 4_1:-76546860 None:intergenic
GTAAGTTTGGAAAATGGGAC+TGG 0.568508 4_1:+76547218 Msa0573360:CDS
TGAACAGAGAAACGTCTCCC+AGG 0.569762 4_1:-76547337 None:intergenic
ATCTCAAGTGTGTCAAGCCT+AGG 0.572917 4_1:+76546862 Msa0573360:CDS
TAGGAACGCTTGGTTAGCTG+TGG 0.580545 4_1:+76546602 Msa0573360:CDS
TATCTGGTGAACGTGATTTG+AGG 0.581565 4_1:-76545432 None:intergenic
ACGAGAGATGGAACAGATGA+GGG 0.582688 4_1:-76545407 None:intergenic
AATCTTGAATCCGTCACCAT+CGG 0.590717 4_1:+76545514 Msa0573360:CDS
GAATGAAATTGAAATCCGTA+AGG 0.594296 4_1:-76545600 None:intergenic
AACGAGAGATGGAACAGATG+AGG 0.597347 4_1:-76545408 None:intergenic
CCCATCTCCATCTAACTCCA+GGG 0.598276 4_1:-76547421 None:intergenic
AACTTAGATACCTTATGTTG+GGG 0.600732 4_1:-76547151 None:intergenic
AGACTGAAGTTTGACAAAGA+AGG 0.601585 4_1:-76546903 None:intergenic
ACTAACAAGTGGAAGAGATG+AGG 0.602954 4_1:-76545717 None:intergenic
AACAACACGCATCGTGATCG+TGG 0.606110 4_1:+76545237 Msa0573360:five_prime_UTR
TAAGTTTGGAAAATGGGACT+GGG 0.606420 4_1:+76547219 Msa0573360:CDS
GCTTCAAGGAAAAGCAACCT+AGG 0.609348 4_1:-76546879 None:intergenic
TGGTGACGGATTCAAGATTG+GGG 0.614137 4_1:-76545510 None:intergenic
ATGAGATTTCCAGCAGCACA+AGG 0.623043 4_1:-76547377 None:intergenic
CTAGATCGGTTGAGTCGTTG+AGG 0.623467 4_1:-76545324 None:intergenic
CAGAGAAACGTCTCCCAGGA+GGG 0.629157 4_1:-76547333 None:intergenic
CTTAGAATTTCTACAACCAG+CGG 0.636022 4_1:-76545298 None:intergenic
AGAGCTTGTTGCACATATCG+TGG 0.636306 4_1:+76547256 Msa0573360:CDS
GATGGAGAGTTCTTTCCGCA+GGG 0.636451 4_1:+76547196 Msa0573360:CDS
GTTTGTAAGATTGGATGATG+AGG 0.640974 4_1:+76546668 Msa0573360:CDS
GTTTAAGGAAAAGGGAGAGT+CGG 0.642281 4_1:+76547497 Msa0573360:three_prime_UTR
AAGAGAACGATCAACGCCGA+TGG 0.643423 4_1:-76545530 None:intergenic
ACAATGCCTTCAGCTTGAAG+CGG 0.645681 4_1:+76546974 Msa0573360:CDS
GATCGGTTGAGTCGTTGAGG+CGG 0.655845 4_1:-76545321 None:intergenic
GGTCAACGAACGAACTTCGG+AGG 0.658236 4_1:-76545386 None:intergenic
CCTGGAGTTAGATGGAGATG+GGG 0.661275 4_1:+76547422 Msa0573360:CDS
TTAATCGAATTGGAAAGCGA+AGG 0.664500 4_1:-76545634 None:intergenic
AATTTCTACAACCAGCGGAG+AGG 0.665838 4_1:-76545293 None:intergenic
GGTGACGGATTCAAGATTGG+GGG 0.683900 4_1:-76545509 None:intergenic
ACAGAGAAACGTCTCCCAGG+AGG 0.695142 4_1:-76547334 None:intergenic
CACAAGGATCAGCATCACCG+TGG 0.702098 4_1:-76547361 None:intergenic
TAACAAGTGGAAGAGATGAG+GGG 0.706106 4_1:-76545715 None:intergenic
AACACGCATCGTGATCGTGG+TGG 0.711865 4_1:+76545240 Msa0573360:five_prime_UTR
GTCTCTCTACAACAACACAA+TGG 0.730203 4_1:-76547034 None:intergenic
ATATGTTCAGAACAAGTGAG+CGG 0.731446 4_1:-76547779 None:intergenic
GAAAGAACAAGTGAACAACA+AGG 0.767673 4_1:-76547823 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATATTTTTAAAGATATTTT+AGG + chr4_1:76546067-76546086 Msa0573360:intron 10.0%
!!! TATCTTTAAAAATATCTACT+TGG - chr4_1:76546063-76546082 None:intergenic 15.0%
!! TATTGTAGATGATTAAAAAT+AGG - chr4_1:76546236-76546255 None:intergenic 15.0%
!! ATATACAAATATATATGAAG+TGG - chr4_1:76547735-76547754 None:intergenic 15.0%
!!! TTTTCTGTCTTAAAGTTAAA+TGG - chr4_1:76545149-76545168 None:intergenic 20.0%
!!! AACTTTAAGACAGAAAAAAT+TGG + chr4_1:76545152-76545171 Msa0573360:five_prime_UTR 20.0%
!! ATTTCAATTTCATTCAAGAT+TGG + chr4_1:76545608-76545627 Msa0573360:CDS 20.0%
!! AATGGACAATAAATAATCAA+AGG - chr4_1:76545763-76545782 None:intergenic 20.0%
!! GTATAAACTCAATTCTAAAT+TGG - chr4_1:76545885-76545904 None:intergenic 20.0%
!! TAATCATCTACAATAGTTAT+AGG + chr4_1:76546241-76546260 Msa0573360:intron 20.0%
!! AATCATCTACAATAGTTATA+GGG + chr4_1:76546242-76546261 Msa0573360:intron 20.0%
!! CATCAATCTATTATAACTAT+GGG - chr4_1:76546437-76546456 None:intergenic 20.0%
!!! AGATTTTGAATAAATTGTGT+TGG + chr4_1:76547582-76547601 Msa0573360:three_prime_UTR 20.0%
! AAAATGTGATATGATAGAGT+CGG + chr4_1:76546100-76546119 Msa0573360:intron 25.0%
! CAGCTATAATAGATAATTGA+CGG + chr4_1:76546170-76546189 Msa0573360:intron 25.0%
! GCATCAATCTATTATAACTA+TGG - chr4_1:76546438-76546457 None:intergenic 25.0%
! GTAAAAAGACTTTACATTGA+TGG + chr4_1:76546459-76546478 Msa0573360:intron 25.0%
!!! ATCCTTTAGAGTTATATTTG+TGG + chr4_1:76546496-76546515 Msa0573360:intron 25.0%
! GATATGAAACTATTAGCAAA+AGG - chr4_1:76546544-76546563 None:intergenic 25.0%
! CAATAAGATTAAGTTCCTTA+AGG - chr4_1:76546725-76546744 None:intergenic 25.0%
! CTTAAGGAACTTAATCTTAT+TGG + chr4_1:76546723-76546742 Msa0573360:CDS 25.0%
! AAACTTAGATACCTTATGTT+GGG - chr4_1:76547155-76547174 None:intergenic 25.0%
! TAAACTTAGATACCTTATGT+TGG - chr4_1:76547156-76547175 None:intergenic 25.0%
!! AACATAAGGTATCTAAGTTT+AGG + chr4_1:76547155-76547174 Msa0573360:CDS 25.0%
!!! TGCATTTATTTTCTCTGTTT+TGG + chr4_1:76547301-76547320 Msa0573360:CDS 25.0%
! TCTGAACATATGTATATTCT+TGG + chr4_1:76547791-76547810 Msa0573360:three_prime_UTR 25.0%
!!! TCTTGAAAACGGTTTTGAAA+GGG - chr4_1:76545474-76545493 None:intergenic 30.0%
!!! TTCTTGAAAACGGTTTTGAA+AGG - chr4_1:76545475-76545494 None:intergenic 30.0%
GAATGAAATTGAAATCCGTA+AGG - chr4_1:76545603-76545622 None:intergenic 30.0%
TAGCATAATTGCAACTACAA+TGG - chr4_1:76545781-76545800 None:intergenic 30.0%
! ATTTCTAATTTTCTATGCCG+TGG + chr4_1:76545995-76546014 Msa0573360:intron 30.0%
!! TGTAAAGTTTTATACCTCCA+CGG - chr4_1:76546015-76546034 None:intergenic 30.0%
TTGGCATGATTAGATTTGAT+AGG - chr4_1:76546044-76546063 None:intergenic 30.0%
! GCTGCTAGATGATTTAAAAT+AGG - chr4_1:76546154-76546173 None:intergenic 30.0%
AGATAATTGACGGTGTAAAA+AGG + chr4_1:76546180-76546199 Msa0573360:intron 30.0%
GTTTGTGTAATAAAGTGCAT+TGG - chr4_1:76546352-76546371 None:intergenic 30.0%
TACAAACTCAAGAGTCAAAT+TGG - chr4_1:76546655-76546674 None:intergenic 30.0%
GATTAAGTTCCTTAAGGTTA+GGG - chr4_1:76546719-76546738 None:intergenic 30.0%
AGATTAAGTTCCTTAAGGTT+AGG - chr4_1:76546720-76546739 None:intergenic 30.0%
GAACTTAATCTTATTGGTGT+TGG + chr4_1:76546729-76546748 Msa0573360:CDS 30.0%
TGAATTATTTCCCCAACATA+AGG + chr4_1:76547141-76547160 Msa0573360:CDS 30.0%
AACTTAGATACCTTATGTTG+GGG - chr4_1:76547154-76547173 None:intergenic 30.0%
! GTATCTAAGTTTAGGTTATG+AGG + chr4_1:76547163-76547182 Msa0573360:CDS 30.0%
CAAGAGTAAACTCAATTTCA+AGG - chr4_1:76547283-76547302 None:intergenic 30.0%
AATATGGAAAGAAGGAATCA+AGG + chr4_1:76547445-76547464 Msa0573360:CDS 30.0%
ATCTAGTGTGTTTAAGGAAA+AGG + chr4_1:76547488-76547507 Msa0573360:exon 30.0%
TCTAGTGTGTTTAAGGAAAA+GGG + chr4_1:76547489-76547508 Msa0573360:exon 30.0%
TTACAGATTGTGTTACAGTT+AGG + chr4_1:76547544-76547563 Msa0573360:three_prime_UTR 30.0%
TTTGAACTACACTTACGAAA+AGG - chr4_1:76547670-76547689 None:intergenic 30.0%
GGTGTTAGAAATTCCTTCAA+TGG + chr4_1:76545261-76545280 Msa0573360:exon 35.0%
CTTAGAATTTCTACAACCAG+CGG - chr4_1:76545301-76545320 None:intergenic 35.0%
!!! CTTGAAAACGGTTTTGAAAG+GGG - chr4_1:76545473-76545492 None:intergenic 35.0%
TCATCTTCAAAGTCGTCAAA+GGG - chr4_1:76545565-76545584 None:intergenic 35.0%
TTCATCTTCAAAGTCGTCAA+AGG - chr4_1:76545566-76545585 None:intergenic 35.0%
ATCTGATGATCTTCACCTTA+CGG + chr4_1:76545585-76545604 Msa0573360:CDS 35.0%
TTAATCGAATTGGAAAGCGA+AGG - chr4_1:76545637-76545656 None:intergenic 35.0%
! ATTTCTGGGTTCAATCTTGT+TGG + chr4_1:76545674-76545693 Msa0573360:CDS 35.0%
ATAAGAAGCTGACTAACAAG+TGG - chr4_1:76545731-76545750 None:intergenic 35.0%
TCAAATGTTTCAGAAGTCTC+TGG - chr4_1:76545829-76545848 None:intergenic 35.0%
ATATGATAGAGTCGGTTGAT+CGG + chr4_1:76546108-76546127 Msa0573360:intron 35.0%
CGCCACAAATATAACTCTAA+AGG - chr4_1:76546501-76546520 None:intergenic 35.0%
TAGAGTTATATTTGTGGCGT+TGG + chr4_1:76546502-76546521 Msa0573360:intron 35.0%
TTTCATATCCTTTGTGCTAC+AGG + chr4_1:76546555-76546574 Msa0573360:intron 35.0%
GACTCTTGAGTTTGTAAGAT+TGG + chr4_1:76546659-76546678 Msa0573360:CDS 35.0%
GTTTGTAAGATTGGATGATG+AGG + chr4_1:76546668-76546687 Msa0573360:CDS 35.0%
TACTTGTTTCCCTAACCTTA+AGG + chr4_1:76546707-76546726 Msa0573360:CDS 35.0%
!! CTTAATCTTATTGGTGTTGG+AGG + chr4_1:76546732-76546751 Msa0573360:CDS 35.0%
AGGTGTGAGAGATTAATCTT+AGG - chr4_1:76546768-76546787 None:intergenic 35.0%
TTCAAATGCTCCTCTTTCTT+TGG + chr4_1:76546809-76546828 Msa0573360:CDS 35.0%
GCAGATATAACCAAAGAAAG+AGG - chr4_1:76546822-76546841 None:intergenic 35.0%
AGATGGAAATCAACAAGACT+TGG - chr4_1:76546846-76546865 None:intergenic 35.0%
AGACTGAAGTTTGACAAAGA+AGG - chr4_1:76546906-76546925 None:intergenic 35.0%
! GAGCTAGTGTTGAAAAATTG+TGG + chr4_1:76546945-76546964 Msa0573360:CDS 35.0%
! TAAGTTTAGGTTATGAGGCT+TGG + chr4_1:76547168-76547187 Msa0573360:CDS 35.0%
TAAGTTTGGAAAATGGGACT+GGG + chr4_1:76547219-76547238 Msa0573360:CDS 35.0%
AGAAGGAATCAAGGATACTT+TGG + chr4_1:76547454-76547473 Msa0573360:CDS 35.0%
! AAGGATACTTTGGTTTCAGA+TGG + chr4_1:76547464-76547483 Msa0573360:CDS 35.0%
ATTGTGTTACAGTTAGGAGT+AGG + chr4_1:76547550-76547569 Msa0573360:three_prime_UTR 35.0%
TTGTGTTACAGTTAGGAGTA+GGG + chr4_1:76547551-76547570 Msa0573360:three_prime_UTR 35.0%
TCGTAAGTGTAGTTCAAACT+AGG + chr4_1:76547672-76547691 Msa0573360:three_prime_UTR 35.0%
GAAACCAGATTAACTAGAAG+TGG + chr4_1:76547694-76547713 Msa0573360:three_prime_UTR 35.0%
ATATGTTCAGAACAAGTGAG+CGG - chr4_1:76547782-76547801 None:intergenic 35.0%
GAAAGAACAAGTGAACAACA+AGG - chr4_1:76547826-76547845 None:intergenic 35.0%
! TGATTTGAGGTAACGAGAGA+TGG - chr4_1:76545422-76545441 None:intergenic 40.0%
TATCTGGTGAACGTGATTTG+AGG - chr4_1:76545435-76545454 None:intergenic 40.0%
GAGAAGGAGGTTCTTGAAAA+CGG - chr4_1:76545485-76545504 None:intergenic 40.0%
!! ATGGTGACGGATTCAAGATT+GGG - chr4_1:76545514-76545533 None:intergenic 40.0%
AATCTTGAATCCGTCACCAT+CGG + chr4_1:76545514-76545533 Msa0573360:CDS 40.0%
AGAGAGCGACTTAATCGAAT+TGG - chr4_1:76545647-76545666 None:intergenic 40.0%
!! GCTCTCTGTTTCTGATTTCT+GGG + chr4_1:76545660-76545679 Msa0573360:CDS 40.0%
TAACAAGTGGAAGAGATGAG+GGG - chr4_1:76545718-76545737 None:intergenic 40.0%
CTAACAAGTGGAAGAGATGA+GGG - chr4_1:76545719-76545738 None:intergenic 40.0%
ACTAACAAGTGGAAGAGATG+AGG - chr4_1:76545720-76545739 None:intergenic 40.0%
GAATTGAATTGTGACCCCAA+TGG + chr4_1:76545847-76545866 Msa0573360:intron 40.0%
! TCTAATTTTCTATGCCGTGG+AGG + chr4_1:76545998-76546017 Msa0573360:intron 40.0%
CACACCTTCAAACTTGTCAA+TGG + chr4_1:76546781-76546800 Msa0573360:CDS 40.0%
CCTTCAAACTTGTCAATGGT+CGG + chr4_1:76546785-76546804 Msa0573360:CDS 40.0%
!! TTGACAAAGAAGGTGCTTCA+AGG - chr4_1:76546896-76546915 None:intergenic 40.0%
GTCTCTCTACAACAACACAA+TGG - chr4_1:76547037-76547056 None:intergenic 40.0%
AAGAACAGAGAGAGTTGTAG+AGG + chr4_1:76547076-76547095 Msa0573360:CDS 40.0%
GTTGATCAAGTAGGAGAGTT+TGG + chr4_1:76547104-76547123 Msa0573360:CDS 40.0%
! CATTTTCCAAACTTACCCTG+CGG - chr4_1:76547214-76547233 None:intergenic 40.0%
GCAGGGTAAGTTTGGAAAAT+GGG + chr4_1:76547213-76547232 Msa0573360:CDS 40.0%
GTAAGTTTGGAAAATGGGAC+TGG + chr4_1:76547218-76547237 Msa0573360:CDS 40.0%
TTAGATGGAGATGGGGAATA+TGG + chr4_1:76547429-76547448 Msa0573360:CDS 40.0%
AGATGGGGAATATGGAAAGA+AGG + chr4_1:76547437-76547456 Msa0573360:CDS 40.0%
GATGGCATCTAGTGTGTTTA+AGG + chr4_1:76547482-76547501 Msa0573360:exon 40.0%
GTTTAAGGAAAAGGGAGAGT+CGG + chr4_1:76547497-76547516 Msa0573360:three_prime_UTR 40.0%
GTCGGTCTTTGAATTGTTGT+AGG + chr4_1:76547515-76547534 Msa0573360:three_prime_UTR 40.0%
! AACGCCACTTCTAGTTAATC+TGG - chr4_1:76547701-76547720 None:intergenic 40.0%
AGATTAACTAGAAGTGGCGT+TGG + chr4_1:76547700-76547719 Msa0573360:three_prime_UTR 40.0%
! TTGTTTGCGCATGTTTTGCG+TGG - chr4_1:76545222-76545241 None:intergenic 45.0%
GAGGGTAGATTCTCCATTGA+AGG - chr4_1:76545277-76545296 None:intergenic 45.0%
ATTTCTACAACCAGCGGAGA+GGG - chr4_1:76545295-76545314 None:intergenic 45.0%
AATTTCTACAACCAGCGGAG+AGG - chr4_1:76545296-76545315 None:intergenic 45.0%
GAGTCTGCATCTTGCTAGAT+CGG - chr4_1:76545341-76545360 None:intergenic 45.0%
ACGAGAGATGGAACAGATGA+GGG - chr4_1:76545410-76545429 None:intergenic 45.0%
AACGAGAGATGGAACAGATG+AGG - chr4_1:76545411-76545430 None:intergenic 45.0%
!! TGGTGACGGATTCAAGATTG+GGG - chr4_1:76545513-76545532 None:intergenic 45.0%
!! GATGGTGACGGATTCAAGAT+TGG - chr4_1:76545515-76545534 None:intergenic 45.0%
!! CGCTCTCTGTTTCTGATTTC+TGG + chr4_1:76545659-76545678 Msa0573360:CDS 45.0%
TGGTTTCTGCAACTGCCATT+GGG - chr4_1:76545865-76545884 None:intergenic 45.0%
TTGGTTTCTGCAACTGCCAT+TGG - chr4_1:76545866-76545885 None:intergenic 45.0%
!! AGAAAGCTTAAAGAGTGCCC+CGG - chr4_1:76546286-76546305 None:intergenic 45.0%
AAATGTTAACGAGTGCCCCT+GGG - chr4_1:76546414-76546433 None:intergenic 45.0%
ATCCTTTGTGCTACAGGCTA+TGG + chr4_1:76546561-76546580 Msa0573360:intron 45.0%
AACCATAGCCTGTAGCACAA+AGG - chr4_1:76546566-76546585 None:intergenic 45.0%
TTGTGCTACAGGCTATGGTT+TGG + chr4_1:76546566-76546585 Msa0573360:intron 45.0%
!! AGGCTATGGTTTGGTGCAAT+TGG + chr4_1:76546575-76546594 Msa0573360:intron 45.0%
!! CTATGGTTTGGTGCAATTGG+AGG + chr4_1:76546578-76546597 Msa0573360:CDS 45.0%
!! GTTTGGTGCAATTGGAGGTT+AGG + chr4_1:76546583-76546602 Msa0573360:CDS 45.0%
AATTGGAGGTTAGGAACGCT+TGG + chr4_1:76546592-76546611 Msa0573360:CDS 45.0%
CCGACCATTGACAAGTTTGA+AGG - chr4_1:76546788-76546807 None:intergenic 45.0%
TAGGCTTGACACACTTGAGA+TGG - chr4_1:76546863-76546882 None:intergenic 45.0%
ATCTCAAGTGTGTCAAGCCT+AGG + chr4_1:76546862-76546881 Msa0573360:CDS 45.0%
GCTTCAAGGAAAAGCAACCT+AGG - chr4_1:76546882-76546901 None:intergenic 45.0%
ACAATGCCTTCAGCTTGAAG+CGG + chr4_1:76546974-76546993 Msa0573360:CDS 45.0%
ACAGAGAGAGTTGTAGAGGT+TGG + chr4_1:76547080-76547099 Msa0573360:CDS 45.0%
! GAGGTTGGAGTTGATCAAGT+AGG + chr4_1:76547095-76547114 Msa0573360:CDS 45.0%
!! TTATGAGGCTTGGCTTGTGA+TGG + chr4_1:76547178-76547197 Msa0573360:CDS 45.0%
TTCTTTCCGCAGGGTAAGTT+TGG + chr4_1:76547205-76547224 Msa0573360:CDS 45.0%
CGCAGGGTAAGTTTGGAAAA+TGG + chr4_1:76547212-76547231 Msa0573360:CDS 45.0%
!! AGAGCTTGTTGCACATATCG+TGG + chr4_1:76547256-76547275 Msa0573360:CDS 45.0%
TTTGGATAAATGCACCCTCC+TGG + chr4_1:76547319-76547338 Msa0573360:CDS 45.0%
TTGGATAAATGCACCCTCCT+GGG + chr4_1:76547320-76547339 Msa0573360:CDS 45.0%
GACGTTTCTCTGTTCATCCA+CGG + chr4_1:76547344-76547363 Msa0573360:CDS 45.0%
ATGAGATTTCCAGCAGCACA+AGG - chr4_1:76547380-76547399 None:intergenic 45.0%
GCAAATTCCCTGGAGTTAGA+TGG + chr4_1:76547414-76547433 Msa0573360:CDS 45.0%
AACAACACGCATCGTGATCG+TGG + chr4_1:76545237-76545256 Msa0573360:five_prime_UTR 50.0%
CTAGATCGGTTGAGTCGTTG+AGG - chr4_1:76545327-76545346 None:intergenic 50.0%
GAGGGTCAACGAACGAACTT+CGG - chr4_1:76545392-76545411 None:intergenic 50.0%
! GTGATGAGGTGCTTGGTATC+TGG - chr4_1:76545451-76545470 None:intergenic 50.0%
!!! CGGTTTTGAAAGGGGTGATG+AGG - chr4_1:76545465-76545484 None:intergenic 50.0%
CGGATTCAAGATTGGGGGAT+CGG - chr4_1:76545507-76545526 None:intergenic 50.0%
!! GGTGACGGATTCAAGATTGG+GGG - chr4_1:76545512-76545531 None:intergenic 50.0%
AAGAGAACGATCAACGCCGA+TGG - chr4_1:76545533-76545552 None:intergenic 50.0%
GGTTTCTGCAACTGCCATTG+GGG - chr4_1:76545864-76545883 None:intergenic 50.0%
GATAGAGTCGGTTGATCGGA+TGG + chr4_1:76546112-76546131 Msa0573360:intron 50.0%
!! AAAGCTTAAAGAGTGCCCCG+GGG - chr4_1:76546284-76546303 None:intergenic 50.0%
!! GAAAGCTTAAAGAGTGCCCC+GGG - chr4_1:76546285-76546304 None:intergenic 50.0%
AAGTGCTTAACCAGTGTCCC+AGG + chr4_1:76546394-76546413 Msa0573360:intron 50.0%
AGTGCTTAACCAGTGTCCCA+GGG + chr4_1:76546395-76546414 Msa0573360:intron 50.0%
GAAATGTTAACGAGTGCCCC+TGG - chr4_1:76546415-76546434 None:intergenic 50.0%
! TAGGAACGCTTGGTTAGCTG+TGG + chr4_1:76546602-76546621 Msa0573360:CDS 50.0%
! AACGCTTGGTTAGCTGTGGA+TGG + chr4_1:76546606-76546625 Msa0573360:CDS 50.0%
TGATGGAGAGTTCTTTCCGC+AGG + chr4_1:76547195-76547214 Msa0573360:CDS 50.0%
GATGGAGAGTTCTTTCCGCA+GGG + chr4_1:76547196-76547215 Msa0573360:CDS 50.0%
TGAACAGAGAAACGTCTCCC+AGG - chr4_1:76547340-76547359 None:intergenic 50.0%
GTGTGCAGAAGCAAATTCCC+TGG + chr4_1:76547404-76547423 Msa0573360:CDS 50.0%
TCCCTGGAGTTAGATGGAGA+TGG + chr4_1:76547420-76547439 Msa0573360:CDS 50.0%
CCCATCTCCATCTAACTCCA+GGG - chr4_1:76547424-76547443 None:intergenic 50.0%
CCCTGGAGTTAGATGGAGAT+GGG + chr4_1:76547421-76547440 Msa0573360:CDS 50.0%
CCTGGAGTTAGATGGAGATG+GGG + chr4_1:76547422-76547441 Msa0573360:CDS 50.0%
AACACGCATCGTGATCGTGG+TGG + chr4_1:76545240-76545259 Msa0573360:five_prime_UTR 55.0%
GATCGGTTGAGTCGTTGAGG+CGG - chr4_1:76545324-76545343 None:intergenic 55.0%
TTCGGAGGAAGCGGAGTTGA+GGG - chr4_1:76545374-76545393 None:intergenic 55.0%
CGAACGAACTTCGGAGGAAG+CGG - chr4_1:76545383-76545402 None:intergenic 55.0%
GGTCAACGAACGAACTTCGG+AGG - chr4_1:76545389-76545408 None:intergenic 55.0%
! GAAAGGGGTGATGAGGTGCT+TGG - chr4_1:76545458-76545477 None:intergenic 55.0%
CAAGATTGGGGGATCGGAGA+AGG - chr4_1:76545501-76545520 None:intergenic 55.0%
ACGATCAACGCCGATGGTGA+CGG - chr4_1:76545527-76545546 None:intergenic 55.0%
! GTCTTGTTAACGAGTGCCCC+CGG + chr4_1:76546264-76546283 Msa0573360:intron 55.0%
TCTTGTTAACGAGTGCCCCC+GGG + chr4_1:76546265-76546284 Msa0573360:intron 55.0%
!! AAGCTTAAAGAGTGCCCCGG+GGG - chr4_1:76546283-76546302 None:intergenic 55.0%
GTGCTTAACCAGTGTCCCAG+GGG + chr4_1:76546396-76546415 Msa0573360:intron 55.0%
CGCATTCCGCTTCAAGCTGA+AGG - chr4_1:76546983-76547002 None:intergenic 55.0%
CAGAGAAACGTCTCCCAGGA+GGG - chr4_1:76547336-76547355 None:intergenic 55.0%
ACAGAGAAACGTCTCCCAGG+AGG - chr4_1:76547337-76547356 None:intergenic 55.0%
CACAAGGATCAGCATCACCG+TGG - chr4_1:76547364-76547383 None:intergenic 55.0%
GATGCTGATCCTTGTGCTGC+TGG + chr4_1:76547368-76547387 Msa0573360:CDS 55.0%
CCCCATCTCCATCTAACTCC+AGG - chr4_1:76547425-76547444 None:intergenic 55.0%
GGAGAATCTACCCTCTCCGC+TGG + chr4_1:76545282-76545301 Msa0573360:CDS 60.0%
CTTCGGAGGAAGCGGAGTTG+AGG - chr4_1:76545375-76545394 None:intergenic 60.0%
GATTGGGGGATCGGAGAAGG+AGG - chr4_1:76545498-76545517 None:intergenic 60.0%
CTTGTTAACGAGTGCCCCCG+GGG + chr4_1:76546266-76546285 Msa0573360:intron 60.0%
AACGAGTGCCCCTGGGACAC+TGG - chr4_1:76546407-76546426 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 76545140 76547893 76545140 ID=Msa0573360;Name=Msa0573360
chr4_1 mRNA 76545140 76547893 76545140 ID=Msa0573360-mRNA-1;Parent=Msa0573360;Name=Msa0573360-mRNA-1;_AED=0.00;_eAED=0.00;_QI=140|1|1|1|1|1|2|400|456
chr4_1 exon 76545140 76545733 76545140 ID=Msa0573360-mRNA-1:exon:26767;Parent=Msa0573360-mRNA-1
chr4_1 exon 76546577 76547893 76546577 ID=Msa0573360-mRNA-1:exon:26768;Parent=Msa0573360-mRNA-1
chr4_1 five_prime_UTR 76545140 76545279 76545140 ID=Msa0573360-mRNA-1:five_prime_utr;Parent=Msa0573360-mRNA-1
chr4_1 CDS 76545280 76545733 76545280 ID=Msa0573360-mRNA-1:cds;Parent=Msa0573360-mRNA-1
chr4_1 CDS 76546577 76547493 76546577 ID=Msa0573360-mRNA-1:cds;Parent=Msa0573360-mRNA-1
chr4_1 three_prime_UTR 76547494 76547893 76547494 ID=Msa0573360-mRNA-1:three_prime_utr;Parent=Msa0573360-mRNA-1
Gene Sequence

>Msa0573360

ATGGAGAATCTACCCTCTCCGCTGGTTGTAGAAATTCTAAGCCGCCTCAACGACTCAACCGATCTAGCAAGATGCAGACTCGTTTCAAACACCCTCAACTCCGCTTCCTCCGAAGTTCGTTCGTTGACCCTCATCTGTTCCATCTCTCGTTACCTCAAATCACGTTCACCAGATACCAAGCACCTCATCACCCCTTTCAAAACCGTTTTCAAGAACCTCCTTCTCCGATCCCCCAATCTTGAATCCGTCACCATCGGCGTTGATCGTTCTCTTGTTGAGATGCCCTTTGACGACTTTGAAGATGAATCTGATGATCTTCACCTTACGGATTTCAATTTCATTCAAGATTGGCTTCCTTCGCTTTCCAATTCGATTAAGTCGCTCTCTGTTTCTGATTTCTGGGTTCAATCTTGTTGGAGACGTTCACGAGCTTTGCCCCTCATCTCTTCCACTTGCTATGGTTTGGTGCAATTGGAGGTTAGGAACGCTTGGTTAGCTGTGGATGGTTTGTGTTTGATGCACACATTGACCAATTTGACTCTTGAGTTTGTAAGATTGGATGATGAGGATTTGAACATGATCAATACTTGTTTCCCTAACCTTAAGGAACTTAATCTTATTGGTGTTGGAGGACTTGAAGAGCCTAAGATTAATCTCTCACACCTTCAAACTTGTCAATGGTCGGTTTCAAATGCTCCTCTTTCTTTGGTTATATCTGCTCCAAGTCTTGTTGATTTCCATCTCAAGTGTGTCAAGCCTAGGTTGCTTTTCCTTGAAGCACCTTCTTTGTCAAACTTCAGTCTTTCTCTTGAGAACACTGATGAGCTAGTGTTGAAAAATTGTGGCAACATACAATGCCTTCAGCTTGAAGCGGAATGCGTCTCTCTTGCTTACATTTTAAGCATGTTTCGCCATTGTGTTGTTGTAGAGAGACTGAGCTTAGATTTTGTGAGAAGAACAGAGAGAGTTGTAGAGGTTGGAGTTGATCAAGTAGGAGAGTTTGGTCTTGATACGTTGTTGAATTATTTCCCCAACATAAGGTATCTAAGTTTAGGTTATGAGGCTTGGCTTGTGATGGAGAGTTCTTTCCGCAGGGTAAGTTTGGAAAATGGGACTGGGATGAAGTCTTTGAAAGAGCTTGTTGCACATATCGTGGTCCTTGAAATTGAGTTTACTCTTGCATTTATTTTCTCTGTTTTGGATAAATGCACCCTCCTGGGAGACGTTTCTCTGTTCATCCACGGTGATGCTGATCCTTGTGCTGCTGGAAATCTCATCTCTGTGTGCAGAAGCAAATTCCCTGGAGTTAGATGGAGATGGGGAATATGGAAAGAAGGAATCAAGGATACTTTGGTTTCAGATGGCATCTAG
Protein sequence

>Msa0573360

MENLPSPLVVEILSRLNDSTDLARCRLVSNTLNSASSEVRSLTLICSISRYLKSRSPDTKHLITPFKTVFKNLLLRSPNLESVTIGVDRSLVEMPFDDFEDESDDLHLTDFNFIQDWLPSLSNSIKSLSVSDFWVQSCWRRSRALPLISSTCYGLVQLEVRNAWLAVDGLCLMHTLTNLTLEFVRLDDEDLNMINTCFPNLKELNLIGVGGLEEPKINLSHLQTCQWSVSNAPLSLVISAPSLVDFHLKCVKPRLLFLEAPSLSNFSLSLENTDELVLKNCGNIQCLQLEAECVSLAYILSMFRHCVVVERLSLDFVRRTERVVEVGVDQVGEFGLDTLLNYFPNIRYLSLGYEAWLVMESSFRRVSLENGTGMKSLKELVAHIVVLEIEFTLAFIFSVLDKCTLLGDVSLFIHGDADPCAAGNLISVCRSKFPGVRWRWGIWKEGIKDTLVSDGI*