AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0576540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0576540 MtrunA17_Chr8g0390121 93.881 621 30 3 1 620 1 614 0.0 1201
Msa0576540 MtrunA17_Chr3g0140131 48.963 627 280 7 1 618 1 596 0.0 590
Msa0576540 MtrunA17_Chr1g0146181 63.270 422 153 2 194 614 165 585 0.0 587
Msa0576540 MtrunA17_Chr3g0140141 63.797 395 142 1 219 613 156 549 0.0 570
Msa0576540 MtrunA17_Chr7g0265381 67.027 370 121 1 244 613 3 371 0.0 546
Msa0576540 MtrunA17_Chr1g0197381 61.765 374 138 1 245 618 182 550 1.71e-174 507
Msa0576540 MtrunA17_Chr4g0055991 61.662 373 138 3 248 615 163 535 1.77e-168 491
Msa0576540 MtrunA17_Chr7g0265451 40.659 455 232 11 172 612 81 511 5.78e-115 353
Msa0576540 MtrunA17_Chr7g0265461 43.501 377 201 4 243 612 23 394 3.33e-109 334
Msa0576540 MtrunA17_Chr3g0131051 41.860 387 206 5 243 618 112 490 1.31e-107 333
Msa0576540 MtrunA17_Chr3g0120131 43.377 385 198 4 243 612 99 478 1.70e-107 332
Msa0576540 MtrunA17_Chr1g0179651 45.455 341 175 5 277 612 1 335 8.42e-104 318
Msa0576540 MtrunA17_Chr3g0119721 45.373 335 175 3 281 612 11 340 2.93e-103 317
Msa0576540 MtrunA17_Chr3g0137491 43.605 344 183 5 275 612 49 387 1.28e-95 298
Msa0576540 MtrunA17_Chr4g0064471 38.875 391 209 8 243 612 85 466 7.21e-88 281
Msa0576540 MtrunA17_Chr4g0064411 39.130 391 215 8 243 618 79 461 4.25e-85 273
Msa0576540 MtrunA17_Chr4g0064461 38.131 396 219 9 243 619 81 469 9.35e-82 265
Msa0576540 MtrunA17_Chr5g0411991 33.526 346 214 7 268 608 73 407 1.49e-63 215
Msa0576540 MtrunA17_Chr7g0253441 35.000 360 191 13 271 611 141 476 9.90e-56 196
Msa0576540 MtrunA17_Chr5g0421571 32.127 221 134 7 268 483 126 335 2.83e-27 113
Msa0576540 MtrunA17_Chr8g0385671 28.693 352 201 14 289 607 50 384 1.58e-26 112
Msa0576540 MtrunA17_Chr8g0385651 27.855 359 195 15 289 607 50 384 1.19e-24 107
Msa0576540 MtrunA17_Chr4g0028201 28.492 358 197 14 289 607 107 444 3.04e-21 97.1
Msa0576540 MtrunA17_Chr2g0320251 28.159 277 155 8 331 578 134 395 8.06e-21 95.5
Msa0576540 MtrunA17_Chr1g0159421 26.959 319 191 11 283 572 68 373 1.60e-18 88.6
Msa0576540 MtrunA17_Chr3g0092341 24.820 278 177 7 329 588 188 451 1.93e-13 73.2
Msa0576540 MtrunA17_Chr3g0122631 25.952 289 199 10 329 607 117 400 3.88e-13 71.6
Msa0576540 MtrunA17_Chr4g0039811 26.619 278 168 9 334 592 185 445 5.46e-13 71.6
Msa0576540 MtrunA17_Chr8g0377331 25.625 320 209 11 307 607 89 398 6.13e-13 71.2
Msa0576540 MtrunA17_Chr4g0043801 26.316 304 177 14 329 608 161 441 8.50e-13 70.9
Msa0576540 MtrunA17_Chr6g0460631 25.704 284 181 10 329 596 184 453 1.44e-11 67.4
Msa0576540 MtrunA17_Chr7g0254391 23.729 295 196 9 329 607 189 470 1.74e-11 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0576540 AT5G19670.1 76.456 412 97 0 207 618 199 610 0.0 701
Msa0576540 AT5G19670.2 76.456 412 97 0 207 618 199 610 0.0 701
Msa0576540 AT5G25820.1 60.850 447 164 6 181 617 209 654 0.0 563
Msa0576540 AT4G32790.1 60.798 426 164 3 191 615 170 593 0.0 554
Msa0576540 AT4G32790.3 60.798 426 164 3 191 615 170 593 0.0 554
Msa0576540 AT4G32790.2 60.798 426 164 3 191 615 170 593 0.0 554
Msa0576540 AT5G11610.1 49.503 503 217 8 143 614 48 544 9.96e-174 505
Msa0576540 AT5G37000.1 55.149 437 190 3 182 618 124 554 4.63e-172 501
Msa0576540 AT4G16745.1 61.789 369 136 3 251 614 162 530 4.15e-169 493
Msa0576540 AT4G16745.2 61.127 355 133 3 251 600 162 516 2.04e-158 468
Msa0576540 AT5G11610.2 45.658 403 183 7 143 515 48 444 5.84e-116 353
Msa0576540 AT5G03795.1 42.440 377 205 4 243 612 140 511 2.84e-105 328
Msa0576540 AT3G07620.1 39.604 404 219 7 227 612 58 454 1.01e-99 312
Msa0576540 AT5G25310.1 37.385 436 247 8 183 612 60 475 2.31e-91 291
Msa0576540 AT5G11130.1 42.017 357 186 7 271 618 131 475 5.71e-90 287
Msa0576540 AT5G11130.2 42.857 343 175 7 285 618 1 331 4.93e-89 280
Msa0576540 AT5G33290.1 40.223 358 189 7 271 612 146 494 1.07e-83 271
Msa0576540 AT3G42180.1 38.187 364 197 7 271 618 116 467 5.62e-82 266
Msa0576540 AT5G20260.2 35.683 454 243 13 174 612 42 461 9.10e-82 265
Msa0576540 AT3G42180.4 38.571 350 187 7 285 618 1 338 2.52e-80 257
Msa0576540 AT5G20260.3 39.412 340 197 4 278 612 21 356 1.50e-78 253
Msa0576540 AT5G20260.1 39.640 333 192 4 285 612 1 329 3.05e-78 251
Msa0576540 AT5G11610.3 42.756 283 132 5 143 396 48 329 1.08e-67 224
Msa0576540 AT4G38040.1 35.311 354 213 8 260 608 75 417 1.75e-66 224
Msa0576540 AT4G38040.2 34.036 332 203 8 260 586 75 395 6.46e-55 192
Msa0576540 AT1G27440.1 28.205 351 204 13 289 607 46 380 7.30e-27 114
Msa0576540 AT5G61840.1 27.557 352 205 14 289 607 49 383 5.97e-25 108
Msa0576540 AT1G67410.1 27.829 327 197 12 307 607 93 406 2.82e-20 94.4
Msa0576540 AT5G22940.2 29.180 305 178 11 331 608 184 477 1.42e-19 92.8
Msa0576540 AT5G22940.1 29.180 305 178 11 331 608 155 448 1.58e-19 92.4
Msa0576540 AT1G21480.2 27.986 293 170 12 288 552 88 367 7.71e-19 89.7
Msa0576540 AT2G28110.1 26.582 316 175 12 289 566 94 390 1.37e-18 89.4
Msa0576540 AT1G21480.1 27.986 293 170 12 288 552 88 367 2.50e-18 88.6
Msa0576540 AT3G03650.1 25.773 291 196 8 329 608 194 475 9.08e-16 80.9
Msa0576540 AT3G45400.1 24.828 290 199 7 329 607 155 436 1.31e-14 77.0
Msa0576540 AT2G35100.1 26.667 300 176 13 329 608 128 403 6.46e-13 71.6
Msa0576540 AT5G16890.1 25.806 279 179 8 329 592 186 451 4.28e-12 69.3

Find 161 sgRNAs with CRISPR-Local

Find 243 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGATTATTGAAGTTATTT+TGG 0.116581 4_1:-80609585 Msa0576540:five_prime_UTR
TTTACTGTTTCAATCAATTT+TGG 0.178927 4_1:-80609454 Msa0576540:CDS
ACATGACCCTTCATTCAATT+TGG 0.224566 4_1:-80606812 Msa0576540:CDS
AGTAGTATTTGTTTCAAGTT+TGG 0.248047 4_1:+80606936 None:intergenic
GACCCTGATATGAAGATATT+TGG 0.253395 4_1:-80607182 Msa0576540:CDS
CATTCCTACATCTTCAATAA+TGG 0.254918 4_1:+80609344 None:intergenic
TCAATGTACTTAACAGTTAC+AGG 0.263665 4_1:-80606539 Msa0576540:three_prime_UTR
GGGCCAAATATCTTCATATC+AGG 0.270993 4_1:+80607179 None:intergenic
TTCTTGTTGCTTGCCATGAT+TGG 0.285262 4_1:-80608150 Msa0576540:CDS
ACAGTTATTTATCTTCAAAT+TGG 0.287986 4_1:+80606495 None:intergenic
GTAACTCAAGGCTTTAAAAT+AGG 0.303212 4_1:-80607365 Msa0576540:CDS
AAGAATTTCCATATCAAGTT+TGG 0.303566 4_1:+80608711 None:intergenic
CTGTCTAAAATTTACTGACT+TGG 0.305149 4_1:-80609669 Msa0576540:five_prime_UTR
GAAAAGTTTCATAGCAGTTT+TGG 0.307842 4_1:-80609184 Msa0576540:CDS
CTATTAACTTCATACCCTTT+GGG 0.312407 4_1:+80607086 None:intergenic
TGATTTCAATAATGCTGTTA+TGG 0.315706 4_1:-80609394 Msa0576540:CDS
GTGGAAATCCAAACTTGATA+TGG 0.325361 4_1:-80608719 Msa0576540:CDS
TCTAACATACGTGAACTAAA+TGG 0.332467 4_1:+80608295 None:intergenic
CTTTCATCAACCTATACTTA+AGG 0.340940 4_1:-80608413 Msa0576540:CDS
GCTATTAACTTCATACCCTT+TGG 0.342007 4_1:+80607085 None:intergenic
ACCGACCGGACCATAGTTTC+AGG 0.343515 4_1:+80607326 None:intergenic
TTCCAATGCTTTAGTAATAT+CGG 0.344343 4_1:+80607212 None:intergenic
ATATGGTGCCTTGTTTCATA+TGG 0.346230 4_1:+80607422 None:intergenic
TTAAGTACATTGACATAATC+TGG 0.346388 4_1:+80606549 None:intergenic
TGTTTCTCTCCCTGAAACTA+TGG 0.347868 4_1:-80607336 Msa0576540:CDS
TAGAACAGGTGGGCCTTTGC+AGG 0.348113 4_1:+80608322 None:intergenic
GTATCAACATTCTTGACTTA+GGG 0.349357 4_1:+80608899 None:intergenic
ATTCAGTACTAGTAACAAAC+TGG 0.350314 4_1:+80606591 None:intergenic
TGTATCAACATTCTTGACTT+AGG 0.353030 4_1:+80608898 None:intergenic
AGCTTGAGATACTTCTCTTC+AGG 0.355805 4_1:+80606906 None:intergenic
CATACATGCAACTGAGTATT+GGG 0.363157 4_1:-80609641 Msa0576540:five_prime_UTR
CTAGAAAGCAGAAGCACATC+TGG 0.364809 4_1:-80606734 Msa0576540:three_prime_UTR
GAGCATGATGGAGTTGGTTT+TGG 0.365949 4_1:-80609057 Msa0576540:CDS
AATCTGCTTTGTGGGTTTAG+TGG 0.369631 4_1:-80609985 Msa0576540:five_prime_UTR
AGGTACGCACTCGTAAAATA+TGG 0.371830 4_1:+80607046 None:intergenic
TTGCGATCTTCAACTTTCTC+AGG 0.374219 4_1:+80609948 None:intergenic
TTTAATCAAATCTGCTTTGT+GGG 0.379184 4_1:-80609993 Msa0576540:five_prime_UTR
TTTCATCAACCTATACTTAA+GGG 0.390149 4_1:-80608412 Msa0576540:CDS
TATCATTACTTTAACAGAAC+TGG 0.396556 4_1:-80608187 Msa0576540:CDS
ATTCCTACATCTTCAATAAT+GGG 0.403772 4_1:+80609345 None:intergenic
GTGCATGTTTCCAGCATAGA+AGG 0.404260 4_1:+80607247 None:intergenic
CATGGCAAGCAACAAGAAAA+TGG 0.406241 4_1:+80608155 None:intergenic
CGAAATCCTCAGAGAGATCT+AGG 0.414552 4_1:-80607302 Msa0576540:CDS
TCCATTCTGGCCTTCTATGC+TGG 0.416141 4_1:-80607257 Msa0576540:CDS
CTTTAATCAAATCTGCTTTG+TGG 0.427928 4_1:-80609994 Msa0576540:five_prime_UTR
TGTGCAACGCCATGAGGCAT+TGG 0.433766 4_1:+80607158 None:intergenic
TCCACAACAAAGGTCCATTC+TGG 0.435157 4_1:-80607270 Msa0576540:CDS
ATCCATCTTTGGAGACGAAA+AGG 0.441637 4_1:-80609131 Msa0576540:CDS
TCTTGTTGCTTGCCATGATT+GGG 0.443147 4_1:-80608149 Msa0576540:intron
TGCTGTTATGGTGGAGAAAG+TGG 0.443234 4_1:-80609382 Msa0576540:CDS
ATGTACGTAACTCTCATAAT+CGG 0.444260 4_1:-80608261 Msa0576540:CDS
GCAATCATTCTATGTTCAAA+AGG 0.447463 4_1:-80608618 Msa0576540:intron
AACTAAATGGCATATAGAAC+AGG 0.456564 4_1:+80608308 None:intergenic
ATATGAAACAAGGCACCATA+TGG 0.460184 4_1:-80607420 Msa0576540:CDS
AATTTCTACAATTTCTCAGT+TGG 0.460305 4_1:-80609106 Msa0576540:CDS
TCATACATGCAACTGAGTAT+TGG 0.463798 4_1:-80609642 Msa0576540:five_prime_UTR
TGTCTAAAATTTACTGACTT+GGG 0.466533 4_1:-80609668 Msa0576540:five_prime_UTR
TAAGTCAAGAATGTTGATAC+AGG 0.466968 4_1:-80608896 Msa0576540:CDS
ATTCCCATTATTGAAGATGT+AGG 0.468389 4_1:-80609348 Msa0576540:CDS
CTTCTGCTTTCTAGTTCAGA+GGG 0.469350 4_1:+80606743 None:intergenic
TATTATACCAAATTGAATGA+AGG 0.472399 4_1:+80606805 None:intergenic
TTTCGTGAGTTATGTCATAT+GGG 0.473284 4_1:-80609522 Msa0576540:CDS
TCCATCTTTGGAGACGAAAA+GGG 0.474825 4_1:-80609130 Msa0576540:CDS
GGCTATATGCGTCGGAGGGG+TGG 0.475205 4_1:-80608390 Msa0576540:CDS
TCAAGTTTGGATTTCCACCT+TGG 0.479561 4_1:+80608724 None:intergenic
TTACTTGAGAAGAAAGATGT+TGG 0.486175 4_1:-80609246 Msa0576540:CDS
GTCCGATATTACTAAAGCAT+TGG 0.487424 4_1:-80607214 Msa0576540:CDS
ATGAGAGTTACGTACATAAA+CGG 0.489748 4_1:+80608267 None:intergenic
TGCGGCGGCGCTCTAGCAAA+TGG 0.494596 4_1:+80608866 None:intergenic
AATATTGAAACATGTTAACT+TGG 0.498323 4_1:+80609544 None:intergenic
GCATATAGCCCCTTAAGTAT+AGG 0.499740 4_1:+80608403 None:intergenic
AAAGTCAACCTTACTCTCCT+TGG 0.505521 4_1:+80609161 None:intergenic
GAAGATCGCAAAACAGAAAG+AGG 0.506249 4_1:-80609936 Msa0576540:five_prime_UTR
GTTAACTTGGCAAAGAACTA+AGG 0.507173 4_1:+80609557 None:intergenic
CCGCACTTCTTCTCGTGCAA+TGG 0.507746 4_1:-80608848 Msa0576540:intron
TCAAGGGAGCATGATGGAGT+TGG 0.511404 4_1:-80609063 Msa0576540:CDS
AAGAGTAGTTTAGTTTCTGA+TGG 0.511732 4_1:-80609309 Msa0576540:CDS
ACATAGAATGATTGCGAAAG+AGG 0.512338 4_1:+80608626 None:intergenic
GTTCAATTTCATTGACTGAT+CGG 0.513596 4_1:-80609023 Msa0576540:CDS
AAATCTCCGTCAGCATTTGA+AGG 0.515165 4_1:-80608236 Msa0576540:CDS
ACCTCAGTGTAGTGAAGACT+CGG 0.517155 4_1:+80609867 None:intergenic
GGGGTGGTTTATGAAATTGA+TGG 0.517173 4_1:-80608374 Msa0576540:CDS
GCTTCTGCTTTCTAGTTCAG+AGG 0.517304 4_1:+80606742 None:intergenic
AGAATGGACCTTTGTTGTGG+AGG 0.521799 4_1:+80607272 None:intergenic
GTGCAACGCCATGAGGCATT+GGG 0.524603 4_1:+80607159 None:intergenic
CACTGCTTCTTAAGCTGTAG+AGG 0.528463 4_1:-80606668 Msa0576540:three_prime_UTR
AAATAAGCAATTTGTTGTGA+AGG 0.529204 4_1:-80608347 Msa0576540:CDS
AAGCTTGAGAAGAAATCAAA+GGG 0.529538 4_1:+80609693 None:intergenic
ATTATACCAAATTGAATGAA+GGG 0.531043 4_1:+80606806 None:intergenic
AGTATAGGTTGATGAAAGAT+CGG 0.532196 4_1:+80608418 None:intergenic
TGAAAGAGGTCGTACTTCAA+AGG 0.533850 4_1:+80606834 None:intergenic
CATAGAATGATTGCGAAAGA+GGG 0.537354 4_1:+80608627 None:intergenic
TTGGAATATCTTTCTCTGCA+AGG 0.537982 4_1:+80606955 None:intergenic
TCCTTGTCATTTGTTACTGT+AGG 0.538036 4_1:+80608661 None:intergenic
TCCAGCATAGAAGGCCAGAA+TGG 0.539537 4_1:+80607256 None:intergenic
CTCTCCCTGAAACTATGGTC+CGG 0.544738 4_1:-80607331 Msa0576540:CDS
AAAGCTTGAGAAGAAATCAA+AGG 0.547635 4_1:+80609692 None:intergenic
GCCAGAATGGACCTTTGTTG+TGG 0.547664 4_1:+80607269 None:intergenic
TACTTTGATTGCAGAAACCA+AGG 0.558872 4_1:-80608741 Msa0576540:intron
GCGAACATCATACCCAATCA+TGG 0.559097 4_1:+80608137 None:intergenic
TCTGATGGTGTTTCTGAACA+TGG 0.566190 4_1:-80609294 Msa0576540:CDS
GGTATGAAGTTAATAGCCCT+CGG 0.566918 4_1:-80607079 Msa0576540:CDS
AATGAAGGGTCATGTGAAAG+AGG 0.567814 4_1:+80606820 None:intergenic
GTGGTTTATGAAATTGATGG+AGG 0.572705 4_1:-80608371 Msa0576540:CDS
CCCTGAAACTATGGTCCGGT+CGG 0.572748 4_1:-80607327 Msa0576540:CDS
GGCCAAATATCTTCATATCA+GGG 0.577327 4_1:+80607180 None:intergenic
GTGTAGGAGCTATGAACTGA+TGG 0.577375 4_1:-80608482 Msa0576540:intron
TTCATCAACCTATACTTAAG+GGG 0.580832 4_1:-80608411 Msa0576540:CDS
AAGGGGCTATATGCGTCGGA+GGG 0.580963 4_1:-80608394 Msa0576540:CDS
TGCAATTCTGATGTAACTCA+AGG 0.581188 4_1:-80607377 Msa0576540:CDS
AATTCTGCATATGCCCCAAA+GGG 0.583083 4_1:-80607100 Msa0576540:CDS
AAGTATCTCAAGCTACAACT+TGG 0.583704 4_1:-80606897 Msa0576540:CDS
GAAAGAATATGACAAGAACT+AGG 0.584243 4_1:-80606631 Msa0576540:three_prime_UTR
TTCTATGCTGGAAACATGCA+CGG 0.584627 4_1:-80607245 Msa0576540:CDS
CCGACCGGACCATAGTTTCA+GGG 0.586993 4_1:+80607327 None:intergenic
TATTAACTTCATACCCTTTG+GGG 0.588331 4_1:+80607087 None:intergenic
GATATTACTAAAGCATTGGA+AGG 0.590151 4_1:-80607210 Msa0576540:CDS
AAATTCTGCATATGCCCCAA+AGG 0.593468 4_1:-80607101 Msa0576540:CDS
AAAGTATACATATACATGGA+AGG 0.600698 4_1:-80608448 Msa0576540:CDS
TAAATGGCATATAGAACAGG+TGG 0.601951 4_1:+80608311 None:intergenic
GTATGAAGTTAATAGCCCTC+GGG 0.605379 4_1:-80607078 Msa0576540:CDS
ACTCAAAGTATACATATACA+TGG 0.608181 4_1:-80608452 Msa0576540:CDS
TGTTGTGAAGGATCCTGCAA+AGG 0.609810 4_1:-80608335 Msa0576540:CDS
ATATTTGGCCCAATGCCTCA+TGG 0.611469 4_1:-80607167 Msa0576540:CDS
TGCCTCAGATAAAAGTGTTG+CGG 0.613422 4_1:-80608962 Msa0576540:CDS
TTTCAATAATGCTGTTATGG+TGG 0.614171 4_1:-80609391 Msa0576540:CDS
ATTGATTCAAGGGAGCATGA+TGG 0.615377 4_1:-80609069 Msa0576540:CDS
TATCAACATTCTTGACTTAG+GGG 0.620041 4_1:+80608900 None:intergenic
ATCAACATTCTTGACTTAGG+GGG 0.620812 4_1:+80608901 None:intergenic
ACTTAAGGGGCTATATGCGT+CGG 0.621663 4_1:-80608398 Msa0576540:CDS
GCAGACAACTTCTTAATCTG+AGG 0.621863 4_1:+80608982 None:intergenic
GAGTTACATCAGAATTGCAG+AGG 0.622250 4_1:+80607381 None:intergenic
TCTGAGGATTTCGTACCGAC+CGG 0.622662 4_1:+80607312 None:intergenic
ATATGGAAATTCTTGCAGCA+AGG 0.626706 4_1:-80608702 Msa0576540:CDS
AGTTACATCAGAATTGCAGA+GGG 0.626825 4_1:+80607382 None:intergenic
AAATTGTCAGATATGATCAC+AGG 0.626917 4_1:+80607026 None:intergenic
TGTAGTCCTTCAAATGCTGA+CGG 0.627267 4_1:+80608230 None:intergenic
CATTACTTTAACAGAACTGG+CGG 0.635287 4_1:-80608184 Msa0576540:CDS
AATATGGCTTCAACTACCCG+AGG 0.640098 4_1:+80607062 None:intergenic
TCCTACAGTAACAAATGACA+AGG 0.644496 4_1:-80608662 Msa0576540:CDS
TTCAGTACTAGTAACAAACT+GGG 0.646271 4_1:+80606592 None:intergenic
CCATCTTTGGAGACGAAAAG+GGG 0.648623 4_1:-80609129 Msa0576540:CDS
TAAGGGGCTATATGCGTCGG+AGG 0.649604 4_1:-80608395 Msa0576540:CDS
GCGGAAAACCTCCACAACAA+AGG 0.655445 4_1:-80607280 Msa0576540:CDS
CCTCAGTGTAGTGAAGACTC+GGG 0.660204 4_1:+80609868 None:intergenic
TGGTGTTTCTGAACATGGAG+TGG 0.660989 4_1:-80609289 Msa0576540:CDS
ATAGAATGATTGCGAAAGAG+GGG 0.662160 4_1:+80608628 None:intergenic
TGTAGGCTCCATATGAAACA+AGG 0.670564 4_1:-80607430 Msa0576540:intron
AGCTTGGAGTTAGATAATGT+AGG 0.672545 4_1:-80609213 Msa0576540:CDS
AGGGGCTATATGCGTCGGAG+GGG 0.678577 4_1:-80608393 Msa0576540:CDS
TTTGATTGCAGAAACCAAGG+TGG 0.685912 4_1:-80608738 Msa0576540:intron
TTGCACGAGAAGAAGTGCGG+CGG 0.687753 4_1:+80608851 None:intergenic
TTGAAATCAGAAGAAGACCA+AGG 0.689924 4_1:+80609408 None:intergenic
CCATTGCACGAGAAGAAGTG+CGG 0.690890 4_1:+80608848 None:intergenic
ATATTACTGTGAGAAAGATG+AGG 0.692407 4_1:-80608933 Msa0576540:CDS
AAATGGCATATAGAACAGGT+GGG 0.698129 4_1:+80608312 None:intergenic
ATATGGCTTCAACTACCCGA+GGG 0.701664 4_1:+80607063 None:intergenic
TTTCCGCCTAGATCTCTCTG+AGG 0.705427 4_1:+80607296 None:intergenic
CAATGAAATTGAACTCTGTG+AGG 0.706222 4_1:+80609032 None:intergenic
CCCGAGTCTTCACTACACTG+AGG 0.771010 4_1:-80609868 Msa0576540:intron
AATCCTCAGAGAGATCTAGG+CGG 0.816666 4_1:-80607299 Msa0576540:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGATTATTGAAGTTATTT+TGG - chr4_1:80606958-80606977 Msa0576540:CDS 15.0%
!!! ATGATTATTGAAGTTATTTT+GGG - chr4_1:80606959-80606978 Msa0576540:CDS 15.0%
!!! AAAGTAAAAATATTGATTCA+AGG - chr4_1:80607463-80607482 Msa0576540:intron 15.0%
!!! AAGTAAAAATATTGATTCAA+GGG - chr4_1:80607464-80607483 Msa0576540:intron 15.0%
!! ATCATAATAGAATAATATCT+TGG + chr4_1:80608472-80608491 None:intergenic 15.0%
!! AAGATATTATTCTATTATGA+TGG - chr4_1:80608471-80608490 Msa0576540:CDS 15.0%
!!! TTTAATACAAAGTATTTGTT+GGG - chr4_1:80608614-80608633 Msa0576540:intron 15.0%
!!! TTTTGTATAAAGTATTTGTT+GGG - chr4_1:80608709-80608728 Msa0576540:CDS 15.0%
!!! TGATTATTGAAGTTATTTTG+GGG - chr4_1:80606960-80606979 Msa0576540:CDS 20.0%
!! AATATTGAAACATGTTAACT+TGG + chr4_1:80607002-80607021 None:intergenic 20.0%
!!! CAAAGATTACTTTTTTTGTT+GGG - chr4_1:80607048-80607067 Msa0576540:CDS 20.0%
!!! TTTACTGTTTCAATCAATTT+TGG - chr4_1:80607089-80607108 Msa0576540:CDS 20.0%
!! TATGTATTAAGACATCAAAT+AGG + chr4_1:80608509-80608528 None:intergenic 20.0%
!!! CTTTAATACAAAGTATTTGT+TGG - chr4_1:80608613-80608632 Msa0576540:intron 20.0%
!!! TTAATACAAAGTATTTGTTG+GGG - chr4_1:80608615-80608634 Msa0576540:intron 20.0%
!! ATACTTTATACAAAAGATGT+AGG + chr4_1:80608704-80608723 None:intergenic 20.0%
!!! CTTTTGTATAAAGTATTTGT+TGG - chr4_1:80608708-80608727 Msa0576540:CDS 20.0%
!!! AAATAGACATCTTTTATACA+GGG - chr4_1:80608751-80608770 Msa0576540:intron 20.0%
!!! ATTTTTCGAGGTTTTAAATT+GGG - chr4_1:80609549-80609568 Msa0576540:exon 20.0%
!! ATTATACCAAATTGAATGAA+GGG + chr4_1:80609740-80609759 None:intergenic 20.0%
!! TATTATACCAAATTGAATGA+AGG + chr4_1:80609741-80609760 None:intergenic 20.0%
! TTTAATCAAATCTGCTTTGT+GGG - chr4_1:80606550-80606569 Msa0576540:three_prime_UTR 25.0%
! AAGAAGAAAAAAACATGAAG+TGG - chr4_1:80606640-80606659 Msa0576540:three_prime_UTR 25.0%
! TTTGAAGGAAAAAGACAAAA+TGG + chr4_1:80606717-80606736 None:intergenic 25.0%
! TTCAGATCAAGTAAATATGA+AGG + chr4_1:80606784-80606803 None:intergenic 25.0%
! AACTTTCTCTAATTATCTCA+AGG - chr4_1:80606811-80606830 Msa0576540:CDS 25.0%
!!! TGTCTAAAATTTACTGACTT+GGG - chr4_1:80606875-80606894 Msa0576540:CDS 25.0%
!!! GAACTTTGAAAGTTCATTTT+TGG - chr4_1:80606931-80606950 Msa0576540:CDS 25.0%
! CCAACAAAAAAAGTAATCTT+TGG + chr4_1:80607050-80607069 None:intergenic 25.0%
!!! CCAAAGATTACTTTTTTTGT+TGG - chr4_1:80607047-80607066 Msa0576540:CDS 25.0%
! TGATTTCAATAATGCTGTTA+TGG - chr4_1:80607149-80607168 Msa0576540:CDS 25.0%
! ATTCCTACATCTTCAATAAT+GGG + chr4_1:80607201-80607220 None:intergenic 25.0%
!! TGTTGGTAAAAATAAAAGCT+TGG - chr4_1:80607314-80607333 Msa0576540:CDS 25.0%
! AATTTCTACAATTTCTCAGT+TGG - chr4_1:80607437-80607456 Msa0576540:intron 25.0%
! AAGAATTTCCATATCAAGTT+TGG + chr4_1:80607835-80607854 None:intergenic 25.0%
! ACTCAAAGTATACATATACA+TGG - chr4_1:80608091-80608110 Msa0576540:intron 25.0%
! AAAGTATACATATACATGGA+AGG - chr4_1:80608095-80608114 Msa0576540:intron 25.0%
! TTTCATCAACCTATACTTAA+GGG - chr4_1:80608131-80608150 Msa0576540:intron 25.0%
!!! AAATAAGCAATTTGTTGTGA+AGG - chr4_1:80608196-80608215 Msa0576540:CDS 25.0%
! TATCATTACTTTAACAGAAC+TGG - chr4_1:80608356-80608375 Msa0576540:CDS 25.0%
! AAAGAGATGTCCAATAAATA+AGG + chr4_1:80608597-80608616 None:intergenic 25.0%
!! GAAATAGACATCTTTTATAC+AGG - chr4_1:80608750-80608769 Msa0576540:intron 25.0%
! CTTTCTATTATTCAAGCTTA+GGG + chr4_1:80608840-80608859 None:intergenic 25.0%
!!! TATCTATTTACTTTTGAACC+AGG - chr4_1:80608954-80608973 Msa0576540:CDS 25.0%
!!! CACATTGCTGATTTTTATAA+TGG + chr4_1:80609028-80609047 None:intergenic 25.0%
! TTCCAATGCTTTAGTAATAT+CGG + chr4_1:80609334-80609353 None:intergenic 25.0%
!! CAATTTAAAACCTCGAAAAA+TGG + chr4_1:80609550-80609569 None:intergenic 25.0%
!!! CATTTTTCGAGGTTTTAAAT+TGG - chr4_1:80609548-80609567 Msa0576540:exon 25.0%
!!! AGTAGTATTTGTTTCAAGTT+TGG + chr4_1:80609610-80609629 None:intergenic 25.0%
! TTAAGTACATTGACATAATC+TGG + chr4_1:80609997-80610016 None:intergenic 25.0%
CTTTAATCAAATCTGCTTTG+TGG - chr4_1:80606549-80606568 Msa0576540:three_prime_UTR 30.0%
GTTGATTGATCTACATTTGA+AGG + chr4_1:80606732-80606751 None:intergenic 30.0%
!!! TCTCAAGGTCCATTTTTTTT+AGG - chr4_1:80606826-80606845 Msa0576540:CDS 30.0%
TCAAAGGGTCCTAAAAAAAA+TGG + chr4_1:80606838-80606857 None:intergenic 30.0%
AAGCTTGAGAAGAAATCAAA+GGG + chr4_1:80606853-80606872 None:intergenic 30.0%
AAAGCTTGAGAAGAAATCAA+AGG + chr4_1:80606854-80606873 None:intergenic 30.0%
! CTGTCTAAAATTTACTGACT+TGG - chr4_1:80606874-80606893 Msa0576540:CDS 30.0%
! TTTTCGTGAGTTATGTCATA+TGG - chr4_1:80607020-80607039 Msa0576540:CDS 30.0%
TTTCGTGAGTTATGTCATAT+GGG - chr4_1:80607021-80607040 Msa0576540:CDS 30.0%
!!! GGAGCTTTTTCATTTTCATA+TGG + chr4_1:80607117-80607136 None:intergenic 30.0%
ATGAAAATGAAAAAGCTCCT+TGG - chr4_1:80607118-80607137 Msa0576540:CDS 30.0%
TTTCAATAATGCTGTTATGG+TGG - chr4_1:80607152-80607171 Msa0576540:CDS 30.0%
ATTCCCATTATTGAAGATGT+AGG - chr4_1:80607195-80607214 Msa0576540:CDS 30.0%
CATTCCTACATCTTCAATAA+TGG + chr4_1:80607202-80607221 None:intergenic 30.0%
! AAGAGTAGTTTAGTTTCTGA+TGG - chr4_1:80607234-80607253 Msa0576540:CDS 30.0%
TTACTTGAGAAGAAAGATGT+TGG - chr4_1:80607297-80607316 Msa0576540:CDS 30.0%
! AACTGCTATGAAACTTTTCT+TGG + chr4_1:80607358-80607377 None:intergenic 30.0%
! GAAAAGTTTCATAGCAGTTT+TGG - chr4_1:80607359-80607378 Msa0576540:CDS 30.0%
! GTTCAATTTCATTGACTGAT+CGG - chr4_1:80607520-80607539 Msa0576540:intron 30.0%
ATATTACTGTGAGAAAGATG+AGG - chr4_1:80607610-80607629 Msa0576540:intron 30.0%
TATCAACATTCTTGACTTAG+GGG + chr4_1:80607646-80607665 None:intergenic 30.0%
GTATCAACATTCTTGACTTA+GGG + chr4_1:80607647-80607666 None:intergenic 30.0%
TGTATCAACATTCTTGACTT+AGG + chr4_1:80607648-80607667 None:intergenic 30.0%
TAAGTCAAGAATGTTGATAC+AGG - chr4_1:80607647-80607666 Msa0576540:intron 30.0%
! TACCTGAAAAATTGTTGTTG+TGG - chr4_1:80607752-80607771 Msa0576540:intron 30.0%
TGCAATCAAAGTAGAATTTC+AGG + chr4_1:80607795-80607814 None:intergenic 30.0%
GCAATCATTCTATGTTCAAA+AGG - chr4_1:80607925-80607944 Msa0576540:intron 30.0%
AACTAAAGTAAGTTCTACTC+TGG - chr4_1:80607984-80608003 Msa0576540:intron 30.0%
TCTTTCCTAATTGTATGTGT+AGG - chr4_1:80608045-80608064 Msa0576540:intron 30.0%
!! AGTATAGGTTGATGAAAGAT+CGG + chr4_1:80608128-80608147 None:intergenic 30.0%
CTTTCATCAACCTATACTTA+AGG - chr4_1:80608130-80608149 Msa0576540:intron 30.0%
TTCATCAACCTATACTTAAG+GGG - chr4_1:80608132-80608151 Msa0576540:intron 30.0%
AACTAAATGGCATATAGAAC+AGG + chr4_1:80608238-80608257 None:intergenic 30.0%
TCTAACATACGTGAACTAAA+TGG + chr4_1:80608251-80608270 None:intergenic 30.0%
ATGAGAGTTACGTACATAAA+CGG + chr4_1:80608279-80608298 None:intergenic 30.0%
ATGTACGTAACTCTCATAAT+CGG - chr4_1:80608282-80608301 Msa0576540:CDS 30.0%
ATCATGCAAGTGGAATTAAA+GGG + chr4_1:80608446-80608465 None:intergenic 30.0%
! AGCGTAACAACCTTATTTAT+TGG - chr4_1:80608584-80608603 Msa0576540:intron 30.0%
CCTTTCTATTATTCAAGCTT+AGG + chr4_1:80608841-80608860 None:intergenic 30.0%
CCTAAGCTTGAATAATAGAA+AGG - chr4_1:80608838-80608857 Msa0576540:intron 30.0%
GGATATTTGATTATGGAGTT+CGG + chr4_1:80608905-80608924 None:intergenic 30.0%
GAACACTGGATATTTGATTA+TGG + chr4_1:80608912-80608931 None:intergenic 30.0%
! AGTTTTACTTCTCATGTTGT+AGG - chr4_1:80609096-80609115 Msa0576540:CDS 30.0%
! CTTTTATGCAGTATTCCATA+TGG + chr4_1:80609141-80609160 None:intergenic 30.0%
!! GTAACTCAAGGCTTTAAAAT+AGG - chr4_1:80609178-80609197 Msa0576540:CDS 30.0%
!! GATATTACTAAAGCATTGGA+AGG - chr4_1:80609333-80609352 Msa0576540:CDS 30.0%
TATTAACTTCATACCCTTTG+GGG + chr4_1:80609459-80609478 None:intergenic 30.0%
CTATTAACTTCATACCCTTT+GGG + chr4_1:80609460-80609479 None:intergenic 30.0%
AAATTGTCAGATATGATCAC+AGG + chr4_1:80609520-80609539 None:intergenic 30.0%
! TTTAAAACCTCGAAAAATGG+CGG + chr4_1:80609547-80609566 None:intergenic 30.0%
! GAGTTCAGAAACATTTTCTT+TGG - chr4_1:80609677-80609696 Msa0576540:five_prime_UTR 30.0%
GAAAGAATATGACAAGAACT+AGG - chr4_1:80609912-80609931 Msa0576540:five_prime_UTR 30.0%
TTCAGTACTAGTAACAAACT+GGG + chr4_1:80609954-80609973 None:intergenic 30.0%
ATTCAGTACTAGTAACAAAC+TGG + chr4_1:80609955-80609974 None:intergenic 30.0%
TCAATGTACTTAACAGTTAC+AGG - chr4_1:80610004-80610023 Msa0576540:five_prime_UTR 30.0%
TGAAGGCAAGAAAACAGAAA+GGG + chr4_1:80606767-80606786 None:intergenic 35.0%
ATGAAGGCAAGAAAACAGAA+AGG + chr4_1:80606768-80606787 None:intergenic 35.0%
TCATACATGCAACTGAGTAT+TGG - chr4_1:80606901-80606920 Msa0576540:CDS 35.0%
CATACATGCAACTGAGTATT+GGG - chr4_1:80606902-80606921 Msa0576540:CDS 35.0%
! GTTAACTTGGCAAAGAACTA+AGG + chr4_1:80606989-80607008 None:intergenic 35.0%
TTGAAATCAGAAGAAGACCA+AGG + chr4_1:80607138-80607157 None:intergenic 35.0%
AGCTTGGAGTTAGATAATGT+AGG - chr4_1:80607330-80607349 Msa0576540:CDS 35.0%
! CTTTTCAGTGAATCCATCTT+TGG - chr4_1:80607401-80607420 Msa0576540:CDS 35.0%
CAATGAAATTGAACTCTGTG+AGG + chr4_1:80607514-80607533 None:intergenic 35.0%
ATCAACATTCTTGACTTAGG+GGG + chr4_1:80607645-80607664 None:intergenic 35.0%
! CTCCACAACAACAATTTTTC+AGG + chr4_1:80607757-80607776 None:intergenic 35.0%
TACTTTGATTGCAGAAACCA+AGG - chr4_1:80607802-80607821 Msa0576540:intron 35.0%
GTGGAAATCCAAACTTGATA+TGG - chr4_1:80607824-80607843 Msa0576540:intron 35.0%
ATATGGAAATTCTTGCAGCA+AGG - chr4_1:80607841-80607860 Msa0576540:intron 35.0%
TCCTACAGTAACAAATGACA+AGG - chr4_1:80607881-80607900 Msa0576540:intron 35.0%
! TCCTTGTCATTTGTTACTGT+AGG + chr4_1:80607885-80607904 None:intergenic 35.0%
ATAGAATGATTGCGAAAGAG+GGG + chr4_1:80607918-80607937 None:intergenic 35.0%
CATAGAATGATTGCGAAAGA+GGG + chr4_1:80607919-80607938 None:intergenic 35.0%
ACATAGAATGATTGCGAAAG+AGG + chr4_1:80607920-80607939 None:intergenic 35.0%
TAGCTCCTACACATACAATT+AGG + chr4_1:80608053-80608072 None:intergenic 35.0%
GTGGTTTATGAAATTGATGG+AGG - chr4_1:80608172-80608191 Msa0576540:CDS 35.0%
AAATGGCATATAGAACAGGT+GGG + chr4_1:80608234-80608253 None:intergenic 35.0%
TAAATGGCATATAGAACAGG+TGG + chr4_1:80608235-80608254 None:intergenic 35.0%
CATTACTTTAACAGAACTGG+CGG - chr4_1:80608359-80608378 Msa0576540:CDS 35.0%
CATCATGCAAGTGGAATTAA+AGG + chr4_1:80608447-80608466 None:intergenic 35.0%
! ACAAAGTATTTGTTGGGGTA+TGG - chr4_1:80608620-80608639 Msa0576540:CDS 35.0%
! ATGGCTCATAATTTAGGACT+CGG - chr4_1:80608639-80608658 Msa0576540:CDS 35.0%
GAGAGATAAAGTAGTTGTAG+AGG + chr4_1:80608667-80608686 None:intergenic 35.0%
! TTTTATACAGGGACAGTTCA+CGG - chr4_1:80608762-80608781 Msa0576540:intron 35.0%
TCACGGTTAATCTTGTAAAC+TGG - chr4_1:80608779-80608798 Msa0576540:intron 35.0%
AAAATGCAAACTGAGAACAC+TGG + chr4_1:80608926-80608945 None:intergenic 35.0%
!! ATATGGTGCCTTGTTTCATA+TGG + chr4_1:80609124-80609143 None:intergenic 35.0%
! ATATGAAACAAGGCACCATA+TGG - chr4_1:80609123-80609142 Msa0576540:CDS 35.0%
AGTTACATCAGAATTGCAGA+GGG + chr4_1:80609164-80609183 None:intergenic 35.0%
TGCAATTCTGATGTAACTCA+AGG - chr4_1:80609166-80609185 Msa0576540:CDS 35.0%
GTCCGATATTACTAAAGCAT+TGG - chr4_1:80609329-80609348 Msa0576540:CDS 35.0%
GACCCTGATATGAAGATATT+TGG - chr4_1:80609361-80609380 Msa0576540:CDS 35.0%
GGCCAAATATCTTCATATCA+GGG + chr4_1:80609366-80609385 None:intergenic 35.0%
GCTATTAACTTCATACCCTT+TGG + chr4_1:80609461-80609480 None:intergenic 35.0%
TTGGAATATCTTTCTCTGCA+AGG + chr4_1:80609591-80609610 None:intergenic 35.0%
AAGTATCTCAAGCTACAACT+TGG - chr4_1:80609646-80609665 Msa0576540:five_prime_UTR 35.0%
ACATGACCCTTCATTCAATT+TGG - chr4_1:80609731-80609750 Msa0576540:intron 35.0%
AATCTGCTTTGTGGGTTTAG+TGG - chr4_1:80606558-80606577 Msa0576540:three_prime_UTR 40.0%
TTGCGATCTTCAACTTTCTC+AGG + chr4_1:80606598-80606617 None:intergenic 40.0%
GAAGATCGCAAAACAGAAAG+AGG - chr4_1:80606607-80606626 Msa0576540:three_prime_UTR 40.0%
! TCTGATGGTGTTTCTGAACA+TGG - chr4_1:80607249-80607268 Msa0576540:CDS 40.0%
!! TTTCATAGCAGTTTTGGCCA+AGG - chr4_1:80607365-80607384 Msa0576540:CDS 40.0%
AAAGTCAACCTTACTCTCCT+TGG + chr4_1:80607385-80607404 None:intergenic 40.0%
! ATCCATCTTTGGAGACGAAA+AGG - chr4_1:80607412-80607431 Msa0576540:CDS 40.0%
! TCCATCTTTGGAGACGAAAA+GGG - chr4_1:80607413-80607432 Msa0576540:CDS 40.0%
! ATTGATTCAAGGGAGCATGA+TGG - chr4_1:80607474-80607493 Msa0576540:intron 40.0%
GCAGACAACTTCTTAATCTG+AGG + chr4_1:80607564-80607583 None:intergenic 40.0%
!! TGCCTCAGATAAAAGTGTTG+CGG - chr4_1:80607581-80607600 Msa0576540:intron 40.0%
TTTGATTGCAGAAACCAAGG+TGG - chr4_1:80607805-80607824 Msa0576540:intron 40.0%
TCAAGTTTGGATTTCCACCT+TGG + chr4_1:80607822-80607841 None:intergenic 40.0%
AGTTCTACTCTGGCATACAT+TGG - chr4_1:80607994-80608013 Msa0576540:intron 40.0%
GCATATAGCCCCTTAAGTAT+AGG + chr4_1:80608143-80608162 None:intergenic 40.0%
GGGGTGGTTTATGAAATTGA+TGG - chr4_1:80608169-80608188 Msa0576540:CDS 40.0%
AAATCTCCGTCAGCATTTGA+AGG - chr4_1:80608307-80608326 Msa0576540:CDS 40.0%
TGTAGTCCTTCAAATGCTGA+CGG + chr4_1:80608316-80608335 None:intergenic 40.0%
CATGGCAAGCAACAAGAAAA+TGG + chr4_1:80608391-80608410 None:intergenic 40.0%
! TTCTTGTTGCTTGCCATGAT+TGG - chr4_1:80608393-80608412 Msa0576540:CDS 40.0%
! TCTTGTTGCTTGCCATGATT+GGG - chr4_1:80608394-80608413 Msa0576540:CDS 40.0%
ATCTTGGTTCATCATGCAAG+TGG + chr4_1:80608456-80608475 None:intergenic 40.0%
CTTATCGACCCTCATCAAAA+AGG + chr4_1:80608566-80608585 None:intergenic 40.0%
TGGGGTATGGCTCATAATTT+AGG - chr4_1:80608633-80608652 Msa0576540:CDS 40.0%
TGTAGGCTCCATATGAAACA+AGG - chr4_1:80609113-80609132 Msa0576540:CDS 40.0%
GAGTTACATCAGAATTGCAG+AGG + chr4_1:80609165-80609184 None:intergenic 40.0%
TGTTTCTCTCCCTGAAACTA+TGG - chr4_1:80609207-80609226 Msa0576540:CDS 40.0%
TTCTATGCTGGAAACATGCA+CGG - chr4_1:80609298-80609317 Msa0576540:CDS 40.0%
GGGCCAAATATCTTCATATC+AGG + chr4_1:80609367-80609386 None:intergenic 40.0%
AAATTCTGCATATGCCCCAA+AGG - chr4_1:80609442-80609461 Msa0576540:CDS 40.0%
AATTCTGCATATGCCCCAAA+GGG - chr4_1:80609443-80609462 Msa0576540:CDS 40.0%
GGTATGAAGTTAATAGCCCT+CGG - chr4_1:80609464-80609483 Msa0576540:CDS 40.0%
GTATGAAGTTAATAGCCCTC+GGG - chr4_1:80609465-80609484 Msa0576540:CDS 40.0%
AGGTACGCACTCGTAAAATA+TGG + chr4_1:80609500-80609519 None:intergenic 40.0%
AGCTTGAGATACTTCTCTTC+AGG + chr4_1:80609640-80609659 None:intergenic 40.0%
TGAAAGAGGTCGTACTTCAA+AGG + chr4_1:80609712-80609731 None:intergenic 40.0%
AATGAAGGGTCATGTGAAAG+AGG + chr4_1:80609726-80609745 None:intergenic 40.0%
!! CTTCTGCTTTCTAGTTCAGA+GGG + chr4_1:80609803-80609822 None:intergenic 40.0%
ACCTCAGTGTAGTGAAGACT+CGG + chr4_1:80606679-80606698 None:intergenic 45.0%
! TGCTGTTATGGTGGAGAAAG+TGG - chr4_1:80607161-80607180 Msa0576540:CDS 45.0%
! TGGTGTTTCTGAACATGGAG+TGG - chr4_1:80607254-80607273 Msa0576540:CDS 45.0%
!! AGTTTTGGCCAAGGAGAGTA+AGG - chr4_1:80607374-80607393 Msa0576540:CDS 45.0%
! CCATCTTTGGAGACGAAAAG+GGG - chr4_1:80607414-80607433 Msa0576540:CDS 45.0%
!! GAGCATGATGGAGTTGGTTT+TGG - chr4_1:80607486-80607505 Msa0576540:intron 45.0%
GTGTAGGAGCTATGAACTGA+TGG - chr4_1:80608061-80608080 Msa0576540:intron 45.0%
ACTTAAGGGGCTATATGCGT+CGG - chr4_1:80608145-80608164 Msa0576540:intron 45.0%
TGTTGTGAAGGATCCTGCAA+AGG - chr4_1:80608208-80608227 Msa0576540:CDS 45.0%
GCGAACATCATACCCAATCA+TGG + chr4_1:80608409-80608428 None:intergenic 45.0%
AAAAAGGCCAGGACAATGCT+AGG + chr4_1:80608550-80608569 None:intergenic 45.0%
!! TTGTCCTGGCCTTTTTGATG+AGG - chr4_1:80608554-80608573 Msa0576540:intron 45.0%
!! TGTCCTGGCCTTTTTGATGA+GGG - chr4_1:80608555-80608574 Msa0576540:intron 45.0%
AAGCAACTCAGCAGAATACC+TGG + chr4_1:80608975-80608994 None:intergenic 45.0%
CGAAATCCTCAGAGAGATCT+AGG - chr4_1:80609241-80609260 Msa0576540:CDS 45.0%
AATCCTCAGAGAGATCTAGG+CGG - chr4_1:80609244-80609263 Msa0576540:CDS 45.0%
! AGAATGGACCTTTGTTGTGG+AGG + chr4_1:80609274-80609293 None:intergenic 45.0%
TCCACAACAAAGGTCCATTC+TGG - chr4_1:80609273-80609292 Msa0576540:CDS 45.0%
GTGCATGTTTCCAGCATAGA+AGG + chr4_1:80609299-80609318 None:intergenic 45.0%
ATATTTGGCCCAATGCCTCA+TGG - chr4_1:80609376-80609395 Msa0576540:CDS 45.0%
! ATTTTGTGTGCAACGCCATG+AGG + chr4_1:80609394-80609413 None:intergenic 45.0%
ATATGGCTTCAACTACCCGA+GGG + chr4_1:80609483-80609502 None:intergenic 45.0%
AATATGGCTTCAACTACCCG+AGG + chr4_1:80609484-80609503 None:intergenic 45.0%
!!! TTTTGTGCCGCCATTTTTCG+AGG - chr4_1:80609537-80609556 Msa0576540:CDS 45.0%
!! GCTTCTGCTTTCTAGTTCAG+AGG + chr4_1:80609804-80609823 None:intergenic 45.0%
! CTAGAAAGCAGAAGCACATC+TGG - chr4_1:80609809-80609828 Msa0576540:intron 45.0%
CACTGCTTCTTAAGCTGTAG+AGG - chr4_1:80609875-80609894 Msa0576540:five_prime_UTR 45.0%
CCTCAGTGTAGTGAAGACTC+GGG + chr4_1:80606678-80606697 None:intergenic 50.0%
! CCCCTTTTCGTCTCCAAAGA+TGG + chr4_1:80607417-80607436 None:intergenic 50.0%
! TCAAGGGAGCATGATGGAGT+TGG - chr4_1:80607480-80607499 Msa0576540:intron 50.0%
! CGCCGCAACACTTTTATCTG+AGG + chr4_1:80607586-80607605 None:intergenic 50.0%
CCATTGCACGAGAAGAAGTG+CGG + chr4_1:80607698-80607717 None:intergenic 50.0%
GTCCTTACCTAGCATTGTCC+TGG - chr4_1:80608540-80608559 Msa0576540:intron 50.0%
CTACATCTCTGACTGCAGAG+CGG + chr4_1:80608872-80608891 None:intergenic 50.0%
CTCTCCCTGAAACTATGGTC+CGG - chr4_1:80609212-80609231 Msa0576540:CDS 50.0%
! TCTGAGGATTTCGTACCGAC+CGG + chr4_1:80609234-80609253 None:intergenic 50.0%
TTTCCGCCTAGATCTCTCTG+AGG + chr4_1:80609250-80609269 None:intergenic 50.0%
GCGGAAAACCTCCACAACAA+AGG - chr4_1:80609263-80609282 Msa0576540:CDS 50.0%
GCCAGAATGGACCTTTGTTG+TGG + chr4_1:80609277-80609296 None:intergenic 50.0%
! TCCATTCTGGCCTTCTATGC+TGG - chr4_1:80609286-80609305 Msa0576540:CDS 50.0%
TCCAGCATAGAAGGCCAGAA+TGG + chr4_1:80609290-80609309 None:intergenic 50.0%
CCCGAGTCTTCACTACACTG+AGG - chr4_1:80606675-80606694 Msa0576540:three_prime_UTR 55.0%
! TTGCACGAGAAGAAGTGCGG+CGG + chr4_1:80607695-80607714 None:intergenic 55.0%
CCGCACTTCTTCTCGTGCAA+TGG - chr4_1:80607695-80607714 Msa0576540:intron 55.0%
TAAGGGGCTATATGCGTCGG+AGG - chr4_1:80608148-80608167 Msa0576540:intron 55.0%
AAGGGGCTATATGCGTCGGA+GGG - chr4_1:80608149-80608168 Msa0576540:intron 55.0%
TAGAACAGGTGGGCCTTTGC+AGG + chr4_1:80608224-80608243 None:intergenic 55.0%
GGCCAGGACAATGCTAGGTA+AGG + chr4_1:80608545-80608564 None:intergenic 55.0%
CGACCCTCATCAAAAAGGCC+AGG + chr4_1:80608561-80608580 None:intergenic 55.0%
CGCTCTGCAGTCAGAGATGT+AGG - chr4_1:80608870-80608889 Msa0576540:CDS 55.0%
CCGACCGGACCATAGTTTCA+GGG + chr4_1:80609219-80609238 None:intergenic 55.0%
CCCTGAAACTATGGTCCGGT+CGG - chr4_1:80609216-80609235 Msa0576540:CDS 55.0%
ACCGACCGGACCATAGTTTC+AGG + chr4_1:80609220-80609239 None:intergenic 55.0%
! GTGCAACGCCATGAGGCATT+GGG + chr4_1:80609387-80609406 None:intergenic 55.0%
TGTGCAACGCCATGAGGCAT+TGG + chr4_1:80609388-80609407 None:intergenic 55.0%
AGGGGCTATATGCGTCGGAG+GGG - chr4_1:80608150-80608169 Msa0576540:CDS 60.0%
TGCGGCGGCGCTCTAGCAAA+TGG + chr4_1:80607680-80607699 None:intergenic 65.0%
GGCTATATGCGTCGGAGGGG+TGG - chr4_1:80608153-80608172 Msa0576540:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 80606509 80610056 80606509 ID=Msa0576540;Name=Msa0576540
chr4_1 mRNA 80606509 80610056 80606509 ID=Msa0576540-mRNA-1;Parent=Msa0576540;Name=Msa0576540-mRNA-1;_AED=0.07;_eAED=0.07;_QI=349|1|1|1|0.75|0.6|5|264|620
chr4_1 exon 80606509 80607447 80606509 ID=Msa0576540-mRNA-1:exon:28902;Parent=Msa0576540-mRNA-1
chr4_1 exon 80608150 80608498 80608150 ID=Msa0576540-mRNA-1:exon:28901;Parent=Msa0576540-mRNA-1
chr4_1 exon 80608619 80608749 80608619 ID=Msa0576540-mRNA-1:exon:28900;Parent=Msa0576540-mRNA-1
chr4_1 exon 80608849 80609717 80608849 ID=Msa0576540-mRNA-1:exon:28899;Parent=Msa0576540-mRNA-1
chr4_1 exon 80609869 80610056 80609869 ID=Msa0576540-mRNA-1:exon:28898;Parent=Msa0576540-mRNA-1
chr4_1 five_prime_UTR 80609869 80610056 80609869 ID=Msa0576540-mRNA-1:five_prime_utr;Parent=Msa0576540-mRNA-1
chr4_1 five_prime_UTR 80609557 80609717 80609557 ID=Msa0576540-mRNA-1:five_prime_utr;Parent=Msa0576540-mRNA-1
chr4_1 CDS 80608849 80609556 80608849 ID=Msa0576540-mRNA-1:cds;Parent=Msa0576540-mRNA-1
chr4_1 CDS 80608619 80608749 80608619 ID=Msa0576540-mRNA-1:cds;Parent=Msa0576540-mRNA-1
chr4_1 CDS 80608150 80608498 80608150 ID=Msa0576540-mRNA-1:cds;Parent=Msa0576540-mRNA-1
chr4_1 CDS 80606773 80607447 80606773 ID=Msa0576540-mRNA-1:cds;Parent=Msa0576540-mRNA-1
chr4_1 three_prime_UTR 80606509 80606772 80606509 ID=Msa0576540-mRNA-1:three_prime_utr;Parent=Msa0576540-mRNA-1
Gene Sequence

>Msa0576540

ATGTTTCAATATTTTCGTGAGTTATGTCATATGGGAAACCAAAGATTACTTTTTTTGTTGGGAGCACTTGCTGTTAATTATTTACTGTTTCAATCAATTTTGGTTCCATATGAAAATGAAAAAGCTCCTTGGTCTTCTTCTGATTTCAATAATGCTGTTATGGTGGAGAAAGTGGAGAAAGTGAATATTCCCATTATTGAAGATGTAGGAATGCATAACTATGCAAAGAGTAGTTTAGTTTCTGATGGTGTTTCTGAACATGGAGTGGATAGAAATGATTTTCATATTTTACTTGAGAAGAAAGATGTTGGTAAAAATAAAAGCTTGGAGTTAGATAATGTAGGTTCCAAGAAAAGTTTCATAGCAGTTTTGGCCAAGGAGAGTAAGGTTGACTTTTCAGTGAATCCATCTTTGGAGACGAAAAGGGGAATTTCTACAATTTCTCAGTTGGTAAAAAGTAAAAATATTGATTCAAGGGAGCATGATGGAGTTGGTTTTGGTGCCTCACAGAGTTCAATTTCATTGACTGATCGGACTCGTCTTGAAAGTTCTCCTCAGATTAAGAAGTTGTCTGCCTCAGATAAAAGTGTTGCGGCGAATAATATTACTGTGAGAAAGATGAGGTGTAATATGCCCCCTAAGTCAAGAATGTTGATACAGGAGATGAACCATTTGCTAGAGCGCCGCCGCACTTCTTCTCGTGCAATGAAACCAAGGTGGAAATCCAAACTTGATATGGAAATTCTTGCAGCAAGGTCAGAGATTGAGCATGCTCCTACAGTAACAAATGACAAGGAACTCTATGCACCCCTCTTTCGCAATCATTCTATGTTCAAAAGGAGCTATGAACTGATGGAACACACACTCAAAGTATACATATACATGGAAGGAAATAAACCGATCTTTCATCAACCTATACTTAAGGGGCTATATGCGTCGGAGGGGTGGTTTATGAAATTGATGGAGGAAAATAAGCAATTTGTTGTGAAGGATCCTGCAAAGGCCCACCTGTTCTATATGCCATTTAGTTCACGTATGTTAGAGTTCTCCGTTTATGTACGTAACTCTCATAATCGGACAAATCTCCGTCAGCATTTGAAGGACTACACAGATAAAATTTCAGCGAAGTATCATTACTTTAACAGAACTGGCGGTGCTGACCATTTTCTTGTTGCTTGCCATGATTGGGCTCCATATGAAACAAGGCACCATATGGAATACTGCATAAAAGCCCTCTGCAATTCTGATGTAACTCAAGGCTTTAAAATAGGAAGAGATGTTTCTCTCCCTGAAACTATGGTCCGGTCGGTACGAAATCCTCAGAGAGATCTAGGCGGAAAACCTCCACAACAAAGGTCCATTCTGGCCTTCTATGCTGGAAACATGCACGGTTATTTGCGTCCGATATTACTAAAGCATTGGAAGGACAAAGACCCTGATATGAAGATATTTGGCCCAATGCCTCATGGCGTTGCACACAAAATGAATTACATCGAACACATGAAGAATAGCAAATTCTGCATATGCCCCAAAGGGTATGAAGTTAATAGCCCTCGGGTAGTTGAAGCCATATTTTACGAGTGCGTACCTGTGATCATATCTGACAATTTTGTGCCGCCATTTTTCGAGGTTTTAAATTGGGATGCATTCTCATTAATCCTTGCAGAGAAAGATATTCCAAACTTGAAACAAATACTACTTTCAGTGCCTGAAGAGAAGTATCTCAAGCTACAACTTGGTGTCAAAAGAGTTCAGAAACATTTTCTTTGGCATACTAAACCTTTGAAGTACGACCTCTTTCACATGACCCTTCATTCAATTTGGTATAATAGAGTGTTTCAAATAAATGTGAGAAAAAAATGA
Protein sequence

>Msa0576540

MFQYFRELCHMGNQRLLFLLGALAVNYLLFQSILVPYENEKAPWSSSDFNNAVMVEKVEKVNIPIIEDVGMHNYAKSSLVSDGVSEHGVDRNDFHILLEKKDVGKNKSLELDNVGSKKSFIAVLAKESKVDFSVNPSLETKRGISTISQLVKSKNIDSREHDGVGFGASQSSISLTDRTRLESSPQIKKLSASDKSVAANNITVRKMRCNMPPKSRMLIQEMNHLLERRRTSSRAMKPRWKSKLDMEILAARSEIEHAPTVTNDKELYAPLFRNHSMFKRSYELMEHTLKVYIYMEGNKPIFHQPILKGLYASEGWFMKLMEENKQFVVKDPAKAHLFYMPFSSRMLEFSVYVRNSHNRTNLRQHLKDYTDKISAKYHYFNRTGGADHFLVACHDWAPYETRHHMEYCIKALCNSDVTQGFKIGRDVSLPETMVRSVRNPQRDLGGKPPQQRSILAFYAGNMHGYLRPILLKHWKDKDPDMKIFGPMPHGVAHKMNYIEHMKNSKFCICPKGYEVNSPRVVEAIFYECVPVIISDNFVPPFFEVLNWDAFSLILAEKDIPNLKQILLSVPEEKYLKLQLGVKRVQKHFLWHTKPLKYDLFHMTLHSIWYNRVFQINVRKK*