AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0573930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0573930 MtrunA17_Chr8g0390121 94.042 621 29 3 1 620 1 614 0.0 1204
Msa0573930 MtrunA17_Chr3g0140131 48.885 628 279 8 1 618 1 596 0.0 592
Msa0573930 MtrunA17_Chr1g0146181 63.507 422 152 2 194 614 165 585 0.0 588
Msa0573930 MtrunA17_Chr3g0140141 63.797 395 142 1 219 613 156 549 0.0 572
Msa0573930 MtrunA17_Chr7g0265381 67.297 370 120 1 244 613 3 371 0.0 548
Msa0573930 MtrunA17_Chr1g0197381 62.032 374 137 1 245 618 182 550 2.97e-175 509
Msa0573930 MtrunA17_Chr4g0055991 61.662 373 138 3 248 615 163 535 4.66e-169 492
Msa0573930 MtrunA17_Chr7g0265451 40.659 455 232 11 172 612 81 511 4.46e-115 353
Msa0573930 MtrunA17_Chr7g0265461 43.501 377 201 4 243 612 23 394 2.96e-109 334
Msa0573930 MtrunA17_Chr3g0131051 41.860 387 206 5 243 618 112 490 1.41e-107 333
Msa0573930 MtrunA17_Chr3g0120131 43.377 385 198 4 243 612 99 478 2.16e-107 332
Msa0573930 MtrunA17_Chr1g0179651 45.455 341 175 5 277 612 1 335 7.24e-104 318
Msa0573930 MtrunA17_Chr3g0119721 45.373 335 175 3 281 612 11 340 3.30e-103 317
Msa0573930 MtrunA17_Chr3g0137491 43.605 344 183 5 275 612 49 387 3.32e-96 300
Msa0573930 MtrunA17_Chr4g0064471 38.875 391 209 8 243 612 85 466 4.72e-88 281
Msa0573930 MtrunA17_Chr4g0064411 39.130 391 215 8 243 618 79 461 2.02e-85 274
Msa0573930 MtrunA17_Chr4g0064461 38.131 396 219 9 243 619 81 469 4.28e-82 266
Msa0573930 MtrunA17_Chr5g0411991 33.526 346 214 7 268 608 73 407 8.05e-64 216
Msa0573930 MtrunA17_Chr7g0253441 35.000 360 191 13 271 611 141 476 2.30e-56 198
Msa0573930 MtrunA17_Chr5g0421571 32.127 221 134 7 268 483 126 335 1.56e-27 114
Msa0573930 MtrunA17_Chr8g0385671 28.693 352 201 14 289 607 50 384 1.44e-26 112
Msa0573930 MtrunA17_Chr8g0385651 27.855 359 195 15 289 607 50 384 1.11e-24 107
Msa0573930 MtrunA17_Chr4g0028201 28.492 358 197 14 289 607 107 444 1.95e-21 97.8
Msa0573930 MtrunA17_Chr2g0320251 28.159 277 155 8 331 578 134 395 5.71e-21 96.3
Msa0573930 MtrunA17_Chr1g0159421 26.959 319 191 11 283 572 68 373 2.57e-18 88.2
Msa0573930 MtrunA17_Chr3g0092341 24.820 278 177 7 329 588 188 451 1.59e-13 73.6
Msa0573930 MtrunA17_Chr4g0039811 26.619 278 168 9 334 592 185 445 6.33e-13 71.6
Msa0573930 MtrunA17_Chr4g0043801 26.129 310 170 14 329 608 161 441 7.07e-13 71.2
Msa0573930 MtrunA17_Chr3g0122631 25.952 289 199 10 329 607 117 400 7.10e-13 70.9
Msa0573930 MtrunA17_Chr8g0377331 25.610 328 199 12 307 607 89 398 7.72e-13 70.9
Msa0573930 MtrunA17_Chr7g0254391 23.729 295 196 9 329 607 189 470 1.03e-11 67.8
Msa0573930 MtrunA17_Chr6g0460631 25.704 284 181 10 329 596 184 453 1.47e-11 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0573930 AT5G19670.1 76.456 412 97 0 207 618 199 610 0.0 703
Msa0573930 AT5G19670.2 76.456 412 97 0 207 618 199 610 0.0 703
Msa0573930 AT5G25820.1 61.074 447 163 6 181 617 209 654 0.0 565
Msa0573930 AT4G32790.1 60.798 426 164 3 191 615 170 593 0.0 555
Msa0573930 AT4G32790.3 60.798 426 164 3 191 615 170 593 0.0 555
Msa0573930 AT4G32790.2 60.798 426 164 3 191 615 170 593 0.0 555
Msa0573930 AT5G11610.1 49.702 503 216 8 143 614 48 544 2.35e-174 506
Msa0573930 AT5G37000.1 54.920 437 191 3 182 618 124 554 4.43e-171 498
Msa0573930 AT4G16745.1 61.789 369 136 3 251 614 162 530 9.58e-170 494
Msa0573930 AT4G16745.2 61.127 355 133 3 251 600 162 516 3.89e-159 469
Msa0573930 AT5G11610.2 45.906 403 182 7 143 515 48 444 1.12e-116 355
Msa0573930 AT5G03795.1 42.440 377 205 4 243 612 140 511 2.78e-105 328
Msa0573930 AT3G07620.1 39.604 404 219 7 227 612 58 454 6.84e-100 312
Msa0573930 AT5G25310.1 37.385 436 247 8 183 612 60 475 8.05e-92 292
Msa0573930 AT5G11130.1 42.017 357 186 7 271 618 131 475 3.18e-90 288
Msa0573930 AT5G11130.2 42.857 343 175 7 285 618 1 331 2.83e-89 280
Msa0573930 AT5G33290.1 40.223 358 189 7 271 612 146 494 5.43e-84 272
Msa0573930 AT3G42180.1 38.187 364 197 7 271 618 116 467 2.08e-82 267
Msa0573930 AT5G20260.2 35.683 454 243 13 174 612 42 461 3.82e-82 266
Msa0573930 AT3G42180.4 38.571 350 187 7 285 618 1 338 1.14e-80 258
Msa0573930 AT5G20260.3 39.412 340 197 4 278 612 21 356 7.63e-79 254
Msa0573930 AT5G20260.1 39.640 333 192 4 285 612 1 329 1.55e-78 252
Msa0573930 AT5G11610.3 43.110 283 131 5 143 396 48 329 1.82e-68 226
Msa0573930 AT4G38040.1 35.311 354 213 8 260 608 75 417 9.08e-67 224
Msa0573930 AT4G38040.2 34.036 332 203 8 260 586 75 395 3.56e-55 193
Msa0573930 AT1G27440.1 28.205 351 204 13 289 607 46 380 6.06e-27 114
Msa0573930 AT5G61840.1 27.401 354 203 14 289 607 49 383 5.86e-25 108
Msa0573930 AT1G67410.1 27.829 327 197 12 307 607 93 406 1.66e-20 95.1
Msa0573930 AT5G22940.2 29.180 305 178 11 331 608 184 477 8.08e-20 93.6
Msa0573930 AT5G22940.1 29.180 305 178 11 331 608 155 448 1.07e-19 92.8
Msa0573930 AT2G28110.1 26.582 316 175 12 289 566 94 390 8.40e-19 90.1
Msa0573930 AT1G21480.2 28.231 294 168 13 288 552 88 367 1.20e-18 89.0
Msa0573930 AT1G21480.1 28.231 294 168 13 288 552 88 367 3.39e-18 88.2
Msa0573930 AT3G03650.1 25.773 291 196 8 329 608 194 475 1.52e-15 80.1
Msa0573930 AT3G45400.1 24.828 290 199 7 329 607 155 436 2.56e-14 76.3
Msa0573930 AT2G35100.1 26.667 300 176 13 329 608 128 403 9.12e-13 71.2
Msa0573930 AT5G16890.1 25.806 279 179 8 329 592 186 451 4.97e-12 69.3

Find 158 sgRNAs with CRISPR-Local

Find 241 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGATTATTGAAGTTATTT+TGG 0.116581 4_1:-77272992 Msa0573930:five_prime_UTR
TTTACTGTTTCAATCAATTT+TGG 0.178927 4_1:-77272861 Msa0573930:CDS
ACATGACCCTTCACTCAATT+TGG 0.220651 4_1:-77270220 Msa0573930:CDS
AGTAGTATTTGTTTCAAGTT+TGG 0.248047 4_1:+77270344 None:intergenic
GACCCTGATATGAAGATATT+TGG 0.253395 4_1:-77270590 Msa0573930:CDS
CATTCCTACATCTTCAATAA+TGG 0.254918 4_1:+77272751 None:intergenic
TCAATGTACTTAACAGTTAC+AGG 0.263665 4_1:-77269947 Msa0573930:three_prime_UTR
GGGCCAAATATCTTCATATC+AGG 0.270993 4_1:+77270587 None:intergenic
TTCTTGTTGCTTGCCATGAT+TGG 0.285262 4_1:-77271558 Msa0573930:CDS
ACAGTTATTTATCTTCAAAT+TGG 0.287986 4_1:+77269903 None:intergenic
GTAACTCAAGGCTTTAAAAT+AGG 0.303212 4_1:-77270773 Msa0573930:CDS
AAGAATTTCCATATCAAGTT+TGG 0.303566 4_1:+77272119 None:intergenic
CTGTCTAAAATTTACTGACT+TGG 0.305149 4_1:-77273076 Msa0573930:five_prime_UTR
GAAAAGTTTCATAGCAGTTT+TGG 0.307842 4_1:-77272591 Msa0573930:CDS
CTATTAACTTCATACCCTTT+GGG 0.312407 4_1:+77270494 None:intergenic
TGATTTCAATAATGCTGTTA+TGG 0.315706 4_1:-77272801 Msa0573930:CDS
GTGGAAATCCAAACTTGATA+TGG 0.325361 4_1:-77272127 Msa0573930:CDS
TCTAACATACGTGAACTAAA+TGG 0.332467 4_1:+77271703 None:intergenic
CTTTCATCAACCTATACTTA+AGG 0.340940 4_1:-77271821 Msa0573930:CDS
GCTATTAACTTCATACCCTT+TGG 0.342007 4_1:+77270493 None:intergenic
ACCGACCGGACCATAGTTTC+AGG 0.343515 4_1:+77270734 None:intergenic
ATATGGTGCCTTGTTTCATA+TGG 0.346230 4_1:+77270830 None:intergenic
TTAAGTACATTGACATAATC+TGG 0.346388 4_1:+77269957 None:intergenic
TGTTTCTCTCCCTGAAACTA+TGG 0.347868 4_1:-77270744 Msa0573930:CDS
TAGAACAGGTGGGCCTTTGC+AGG 0.348113 4_1:+77271730 None:intergenic
GTATCAACATTCTTGACTTA+GGG 0.349357 4_1:+77272306 None:intergenic
TGTATCAACATTCTTGACTT+AGG 0.353030 4_1:+77272305 None:intergenic
AGCTTGAGATACTTCTCTTC+AGG 0.355805 4_1:+77270314 None:intergenic
CATACATGCAACTGAGTATT+GGG 0.363157 4_1:-77273048 Msa0573930:five_prime_UTR
TTCCAATGCTTTAGCAATAT+CGG 0.363285 4_1:+77270620 None:intergenic
CTAGAAAGCAGAAGCACATC+TGG 0.364809 4_1:-77270142 Msa0573930:three_prime_UTR
GAGCATGATGGAGTTGGTTT+TGG 0.365949 4_1:-77272464 Msa0573930:CDS
AATCTGCTTTGTGGGTTTAG+TGG 0.369631 4_1:-77273392 Msa0573930:five_prime_UTR
AGGTACGCACTCGTAAAATA+TGG 0.371830 4_1:+77270454 None:intergenic
TTGCGATCTTCAACTTTCTC+AGG 0.374219 4_1:+77273355 None:intergenic
TTTAATCAAATCTGCTTTGT+GGG 0.379184 4_1:-77273400 Msa0573930:five_prime_UTR
TTTCATCAACCTATACTTAA+GGG 0.390149 4_1:-77271820 Msa0573930:CDS
TATCATTACTTTAACAGAAC+TGG 0.396556 4_1:-77271595 Msa0573930:CDS
ATTCCTACATCTTCAATAAT+GGG 0.403772 4_1:+77272752 None:intergenic
GTGCATGTTTCCAGCATAGA+AGG 0.404260 4_1:+77270655 None:intergenic
CATGGCAAGCAACAAGAAAA+TGG 0.406241 4_1:+77271563 None:intergenic
CGAAATCCTCAGAGAGATCT+AGG 0.414552 4_1:-77270710 Msa0573930:CDS
TCCATTCTGGCCTTCTATGC+TGG 0.416141 4_1:-77270665 Msa0573930:CDS
CTTTAATCAAATCTGCTTTG+TGG 0.427928 4_1:-77273401 Msa0573930:five_prime_UTR
TGTGCAACGCCATGAGGCAT+TGG 0.433766 4_1:+77270566 None:intergenic
TCCACAACAAAGGTCCATTC+TGG 0.435157 4_1:-77270678 Msa0573930:CDS
ATCCATCTTTGGAGACGAAA+AGG 0.441637 4_1:-77272538 Msa0573930:CDS
TCTTGTTGCTTGCCATGATT+GGG 0.443147 4_1:-77271557 Msa0573930:intron
TGCTGTTATGGTGGAGAAAG+TGG 0.443234 4_1:-77272789 Msa0573930:CDS
ATGTACGTAACTCTCATAAT+CGG 0.444260 4_1:-77271669 Msa0573930:CDS
GCAATCATTCTATGTTCAAA+AGG 0.447463 4_1:-77272026 Msa0573930:intron
AACTAAATGGCATATAGAAC+AGG 0.456564 4_1:+77271716 None:intergenic
ATATGAAACAAGGCACCATA+TGG 0.460184 4_1:-77270828 Msa0573930:CDS
AATTTCTACAATTTCTCAGT+TGG 0.460305 4_1:-77272513 Msa0573930:CDS
TCATACATGCAACTGAGTAT+TGG 0.463798 4_1:-77273049 Msa0573930:five_prime_UTR
TGTCTAAAATTTACTGACTT+GGG 0.466533 4_1:-77273075 Msa0573930:five_prime_UTR
TAAGTCAAGAATGTTGATAC+AGG 0.466968 4_1:-77272303 Msa0573930:CDS
ATTCCCATTATTGAAGATGT+AGG 0.468389 4_1:-77272755 Msa0573930:CDS
CTTCTGCTTTCTAGTTCAGA+GGG 0.469350 4_1:+77270151 None:intergenic
TTTCGTGAGTTATGTCATAT+GGG 0.473284 4_1:-77272929 Msa0573930:CDS
TCCATCTTTGGAGACGAAAA+GGG 0.474825 4_1:-77272537 Msa0573930:CDS
GGCTATATGCGTCGGAGGGG+TGG 0.475205 4_1:-77271798 Msa0573930:CDS
TCAAGTTTGGATTTCCACCT+TGG 0.479561 4_1:+77272132 None:intergenic
TTACTTGAGAAGAAAGATGT+TGG 0.486175 4_1:-77272653 Msa0573930:CDS
ATGAGAGTTACGTACATAAA+CGG 0.489748 4_1:+77271675 None:intergenic
TGCGGCGGCGCTCTAGCAAA+TGG 0.494596 4_1:+77272273 None:intergenic
AATATTGAAACATGTTAACT+TGG 0.498323 4_1:+77272951 None:intergenic
GCATATAGCCCCTTAAGTAT+AGG 0.499740 4_1:+77271811 None:intergenic
AAAGTCAACCTTACTCTCCT+TGG 0.505521 4_1:+77272568 None:intergenic
GAAGATCGCAAAACAGAAAG+AGG 0.506249 4_1:-77273343 Msa0573930:five_prime_UTR
GTTAACTTGGCAAAGAACTA+AGG 0.507173 4_1:+77272964 None:intergenic
CCGCACTTCTTCTCGTGCAA+TGG 0.507746 4_1:-77272255 Msa0573930:intron
TCAAGGGAGCATGATGGAGT+TGG 0.511404 4_1:-77272470 Msa0573930:CDS
GTCCGATATTGCTAAAGCAT+TGG 0.511437 4_1:-77270622 Msa0573930:CDS
AAGAGTAGTTTAGTTTCTGA+TGG 0.511732 4_1:-77272716 Msa0573930:CDS
ACATAGAATGATTGCGAAAG+AGG 0.512338 4_1:+77272034 None:intergenic
GTTCAATTTCATTGACTGAT+CGG 0.513596 4_1:-77272430 Msa0573930:CDS
GGGGTGGTTTATGAAATTGA+TGG 0.517173 4_1:-77271782 Msa0573930:CDS
GCTTCTGCTTTCTAGTTCAG+AGG 0.517304 4_1:+77270150 None:intergenic
AGAATGGACCTTTGTTGTGG+AGG 0.521799 4_1:+77270680 None:intergenic
GTGCAACGCCATGAGGCATT+GGG 0.524603 4_1:+77270567 None:intergenic
CACTGCTTCTTAAGCTGTAG+AGG 0.528463 4_1:-77270076 Msa0573930:three_prime_UTR
AAATCTCCGTCAGTATTTGA+AGG 0.528805 4_1:-77271644 Msa0573930:CDS
AAATAAGCAATTTGTTGTGA+AGG 0.529204 4_1:-77271755 Msa0573930:CDS
AAGCTTGAGAAGAAATCAAA+GGG 0.529538 4_1:+77273100 None:intergenic
AGTATAGGTTGATGAAAGAT+CGG 0.532196 4_1:+77271826 None:intergenic
TGAAAGAGGTCGTACTTCAA+AGG 0.533850 4_1:+77270242 None:intergenic
CATAGAATGATTGCGAAAGA+GGG 0.537354 4_1:+77272035 None:intergenic
TTGGAATATCTTTCTCTGCA+AGG 0.537982 4_1:+77270363 None:intergenic
TCCTTGTCATTTGTTACTGT+AGG 0.538036 4_1:+77272069 None:intergenic
TCCAGCATAGAAGGCCAGAA+TGG 0.539537 4_1:+77270664 None:intergenic
ATTATACCAAATTGAGTGAA+GGG 0.540646 4_1:+77270214 None:intergenic
CTCTCCCTGAAACTATGGTC+CGG 0.544738 4_1:-77270739 Msa0573930:CDS
AAAGCTTGAGAAGAAATCAA+AGG 0.547635 4_1:+77273099 None:intergenic
GCCAGAATGGACCTTTGTTG+TGG 0.547664 4_1:+77270677 None:intergenic
TACTTTGATTGCAGAAACCA+AGG 0.558872 4_1:-77272149 Msa0573930:intron
GCGAACATCATACCCAATCA+TGG 0.559097 4_1:+77271545 None:intergenic
TCTGATGGTGTTTCTGAACA+TGG 0.566190 4_1:-77272701 Msa0573930:CDS
GGTATGAAGTTAATAGCCCT+CGG 0.566918 4_1:-77270487 Msa0573930:CDS
AGTGAAGGGTCATGTGAAAG+AGG 0.570110 4_1:+77270228 None:intergenic
GTGGTTTATGAAATTGATGG+AGG 0.572705 4_1:-77271779 Msa0573930:CDS
CCCTGAAACTATGGTCCGGT+CGG 0.572748 4_1:-77270735 Msa0573930:CDS
GGCCAAATATCTTCATATCA+GGG 0.577327 4_1:+77270588 None:intergenic
GTGTAGGAGCTATGAACTGA+TGG 0.577375 4_1:-77271890 Msa0573930:intron
TTCATCAACCTATACTTAAG+GGG 0.580832 4_1:-77271819 Msa0573930:CDS
AAGGGGCTATATGCGTCGGA+GGG 0.580963 4_1:-77271802 Msa0573930:CDS
TGCAATTCTGATGTAACTCA+AGG 0.581188 4_1:-77270785 Msa0573930:CDS
AATTCTGCATATGCCCCAAA+GGG 0.583083 4_1:-77270508 Msa0573930:CDS
AAGTATCTCAAGCTACAACT+TGG 0.583704 4_1:-77270305 Msa0573930:CDS
GAAAGAATATGACAAGAACT+AGG 0.584243 4_1:-77270039 Msa0573930:three_prime_UTR
TTCTATGCTGGAAACATGCA+CGG 0.584627 4_1:-77270653 Msa0573930:CDS
CCGACCGGACCATAGTTTCA+GGG 0.586993 4_1:+77270735 None:intergenic
TATTATACCAAATTGAGTGA+AGG 0.587349 4_1:+77270213 None:intergenic
TATTAACTTCATACCCTTTG+GGG 0.588331 4_1:+77270495 None:intergenic
AAATTCTGCATATGCCCCAA+AGG 0.593468 4_1:-77270509 Msa0573930:CDS
GATATTGCTAAAGCATTGGA+AGG 0.596814 4_1:-77270618 Msa0573930:CDS
AAAGTATACATATACATGGA+AGG 0.600698 4_1:-77271856 Msa0573930:CDS
TAAATGGCATATAGAACAGG+TGG 0.601951 4_1:+77271719 None:intergenic
GTATGAAGTTAATAGCCCTC+GGG 0.605379 4_1:-77270486 Msa0573930:CDS
ACTCAAAGTATACATATACA+TGG 0.608181 4_1:-77271860 Msa0573930:CDS
TGTTGTGAAGGATCCTGCAA+AGG 0.609810 4_1:-77271743 Msa0573930:CDS
ATATTTGGCCCAATGCCTCA+TGG 0.611469 4_1:-77270575 Msa0573930:CDS
TGCCTCAGATAAAAGTGTTG+CGG 0.613422 4_1:-77272369 Msa0573930:CDS
TTTCAATAATGCTGTTATGG+TGG 0.614171 4_1:-77272798 Msa0573930:CDS
ATTGATTCAAGGGAGCATGA+TGG 0.615377 4_1:-77272476 Msa0573930:CDS
TATCAACATTCTTGACTTAG+GGG 0.620041 4_1:+77272307 None:intergenic
ATCAACATTCTTGACTTAGG+GGG 0.620812 4_1:+77272308 None:intergenic
ACTTAAGGGGCTATATGCGT+CGG 0.621663 4_1:-77271806 Msa0573930:CDS
GCAGACAACTTCTTAATCTG+AGG 0.621863 4_1:+77272389 None:intergenic
GAGTTACATCAGAATTGCAG+AGG 0.622250 4_1:+77270789 None:intergenic
TCTGAGGATTTCGTACCGAC+CGG 0.622662 4_1:+77270720 None:intergenic
ATATGGAAATTCTTGCAGCA+AGG 0.626706 4_1:-77272110 Msa0573930:CDS
AGTTACATCAGAATTGCAGA+GGG 0.626825 4_1:+77270790 None:intergenic
AAATTGTCAGATATGATCAC+AGG 0.626917 4_1:+77270434 None:intergenic
CATTACTTTAACAGAACTGG+CGG 0.635287 4_1:-77271592 Msa0573930:CDS
AATATGGCTTCAACTACCCG+AGG 0.640098 4_1:+77270470 None:intergenic
TCCTACAGTAACAAATGACA+AGG 0.644496 4_1:-77272070 Msa0573930:CDS
TGTAGTCCTTCAAATACTGA+CGG 0.644705 4_1:+77271638 None:intergenic
TTCAGTACTAGTAACAAACT+AGG 0.644759 4_1:+77270000 None:intergenic
CCATCTTTGGAGACGAAAAG+GGG 0.648623 4_1:-77272536 Msa0573930:CDS
TAAGGGGCTATATGCGTCGG+AGG 0.649604 4_1:-77271803 Msa0573930:CDS
GCGGAAAACCTCCACAACAA+AGG 0.655445 4_1:-77270688 Msa0573930:CDS
TGGTGTTTCTGAACATGGAG+TGG 0.660989 4_1:-77272696 Msa0573930:CDS
ATAGAATGATTGCGAAAGAG+GGG 0.662160 4_1:+77272036 None:intergenic
TGTAGGCTCCATATGAAACA+AGG 0.670564 4_1:-77270838 Msa0573930:intron
AGCTTGGAGTTAGATAATGT+AGG 0.672545 4_1:-77272620 Msa0573930:CDS
AGGGGCTATATGCGTCGGAG+GGG 0.678577 4_1:-77271801 Msa0573930:CDS
TTTGATTGCAGAAACCAAGG+TGG 0.685912 4_1:-77272146 Msa0573930:intron
TTGCACGAGAAGAAGTGCGG+CGG 0.687753 4_1:+77272258 None:intergenic
TTGAAATCAGAAGAAGACCA+AGG 0.689924 4_1:+77272815 None:intergenic
CCATTGCACGAGAAGAAGTG+CGG 0.690890 4_1:+77272255 None:intergenic
ATATTACTGTGAGAAAGATG+AGG 0.692407 4_1:-77272340 Msa0573930:CDS
AAATGGCATATAGAACAGGT+GGG 0.698129 4_1:+77271720 None:intergenic
ATATGGCTTCAACTACCCGA+GGG 0.701664 4_1:+77270471 None:intergenic
TTTCCGCCTAGATCTCTCTG+AGG 0.705427 4_1:+77270704 None:intergenic
CAATGAAATTGAACTCTGTG+AGG 0.706222 4_1:+77272439 None:intergenic
CTCGAGTCTTCACTACACTG+AGG 0.771010 4_1:-77273275 Msa0573930:intron
AATCCTCAGAGAGATCTAGG+CGG 0.816666 4_1:-77270707 Msa0573930:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGATTATTGAAGTTATTT+TGG - chr4_1:77270366-77270385 Msa0573930:CDS 15.0%
!!! ATGATTATTGAAGTTATTTT+GGG - chr4_1:77270367-77270386 Msa0573930:CDS 15.0%
!!! AAAGTAAAAATATTGATTCA+AGG - chr4_1:77270871-77270890 Msa0573930:intron 15.0%
!!! AAGTAAAAATATTGATTCAA+GGG - chr4_1:77270872-77270891 Msa0573930:intron 15.0%
!! ATCATAATAGAATAATATCT+TGG + chr4_1:77271879-77271898 None:intergenic 15.0%
!! AAGATATTATTCTATTATGA+TGG - chr4_1:77271878-77271897 Msa0573930:CDS 15.0%
!!! TTTAATACAAAGTATTTGTT+GGG - chr4_1:77272021-77272040 Msa0573930:intron 15.0%
!!! TTTTGTATAAAGTATTTGTT+GGG - chr4_1:77272116-77272135 Msa0573930:CDS 15.0%
!!! TGATTATTGAAGTTATTTTG+GGG - chr4_1:77270368-77270387 Msa0573930:CDS 20.0%
!! AATATTGAAACATGTTAACT+TGG + chr4_1:77270410-77270429 None:intergenic 20.0%
!!! CAAAGATTACTTTTTTTGTT+GGG - chr4_1:77270456-77270475 Msa0573930:CDS 20.0%
!!! TTTACTGTTTCAATCAATTT+TGG - chr4_1:77270497-77270516 Msa0573930:CDS 20.0%
!! TATGTATTAAGACATCAAAT+AGG + chr4_1:77271916-77271935 None:intergenic 20.0%
!!! CTTTAATACAAAGTATTTGT+TGG - chr4_1:77272020-77272039 Msa0573930:intron 20.0%
!!! TTAATACAAAGTATTTGTTG+GGG - chr4_1:77272022-77272041 Msa0573930:intron 20.0%
!! ATACTTTATACAAAAGATGT+AGG + chr4_1:77272111-77272130 None:intergenic 20.0%
!!! CTTTTGTATAAAGTATTTGT+TGG - chr4_1:77272115-77272134 Msa0573930:CDS 20.0%
!!! AAATAGACATCTTTTATACA+GGG - chr4_1:77272158-77272177 Msa0573930:intron 20.0%
!!! ATTTTTCGAGGTTTTAAATT+GGG - chr4_1:77272956-77272975 Msa0573930:exon 20.0%
! TTTAATCAAATCTGCTTTGT+GGG - chr4_1:77269958-77269977 Msa0573930:three_prime_UTR 25.0%
! AAGAAGAAAAAAACATGAAG+TGG - chr4_1:77270048-77270067 Msa0573930:three_prime_UTR 25.0%
! TTTGAAGGAAAAAGACAAAA+TGG + chr4_1:77270125-77270144 None:intergenic 25.0%
! TTCAGATCAAGTAAATATGA+AGG + chr4_1:77270192-77270211 None:intergenic 25.0%
! AACTTTCTCTAATTATCTCA+AGG - chr4_1:77270219-77270238 Msa0573930:CDS 25.0%
!!! TGTCTAAAATTTACTGACTT+GGG - chr4_1:77270283-77270302 Msa0573930:CDS 25.0%
!!! GAACTTTGAAAGTTCATTTT+TGG - chr4_1:77270339-77270358 Msa0573930:CDS 25.0%
! CCAACAAAAAAAGTAATCTT+TGG + chr4_1:77270458-77270477 None:intergenic 25.0%
!!! CCAAAGATTACTTTTTTTGT+TGG - chr4_1:77270455-77270474 Msa0573930:CDS 25.0%
! TGATTTCAATAATGCTGTTA+TGG - chr4_1:77270557-77270576 Msa0573930:CDS 25.0%
! ATTCCTACATCTTCAATAAT+GGG + chr4_1:77270609-77270628 None:intergenic 25.0%
!! TGTTGGTAAAAATAAAAGCT+TGG - chr4_1:77270722-77270741 Msa0573930:CDS 25.0%
! AATTTCTACAATTTCTCAGT+TGG - chr4_1:77270845-77270864 Msa0573930:intron 25.0%
! AAGAATTTCCATATCAAGTT+TGG + chr4_1:77271242-77271261 None:intergenic 25.0%
! ACTCAAAGTATACATATACA+TGG - chr4_1:77271498-77271517 Msa0573930:intron 25.0%
! AAAGTATACATATACATGGA+AGG - chr4_1:77271502-77271521 Msa0573930:intron 25.0%
! TTTCATCAACCTATACTTAA+GGG - chr4_1:77271538-77271557 Msa0573930:intron 25.0%
!!! AAATAAGCAATTTGTTGTGA+AGG - chr4_1:77271603-77271622 Msa0573930:CDS 25.0%
! TATCATTACTTTAACAGAAC+TGG - chr4_1:77271763-77271782 Msa0573930:CDS 25.0%
! AAAGAGATGTCCAATAAATA+AGG + chr4_1:77272004-77272023 None:intergenic 25.0%
!! GAAATAGACATCTTTTATAC+AGG - chr4_1:77272157-77272176 Msa0573930:intron 25.0%
! CTTTCTATTATTCAAGCTTA+GGG + chr4_1:77272247-77272266 None:intergenic 25.0%
!!! TATCTATTTACTTTTGAACC+AGG - chr4_1:77272361-77272380 Msa0573930:CDS 25.0%
!!! CACATTGCTGATTTTTATAA+TGG + chr4_1:77272435-77272454 None:intergenic 25.0%
!! CAATTTAAAACCTCGAAAAA+TGG + chr4_1:77272957-77272976 None:intergenic 25.0%
!!! CATTTTTCGAGGTTTTAAAT+TGG - chr4_1:77272955-77272974 Msa0573930:exon 25.0%
!!! AGTAGTATTTGTTTCAAGTT+TGG + chr4_1:77273017-77273036 None:intergenic 25.0%
! ATTATACCAAATTGAGTGAA+GGG + chr4_1:77273147-77273166 None:intergenic 25.0%
! TATTATACCAAATTGAGTGA+AGG + chr4_1:77273148-77273167 None:intergenic 25.0%
! TTAAGTACATTGACATAATC+TGG + chr4_1:77273404-77273423 None:intergenic 25.0%
CTTTAATCAAATCTGCTTTG+TGG - chr4_1:77269957-77269976 Msa0573930:three_prime_UTR 30.0%
GTTGATTGATCTACATTTGA+AGG + chr4_1:77270140-77270159 None:intergenic 30.0%
!!! TCTCAAGGTCCATTTTTTTT+AGG - chr4_1:77270234-77270253 Msa0573930:CDS 30.0%
TCAAAGGGTCCTAAAAAAAA+TGG + chr4_1:77270246-77270265 None:intergenic 30.0%
AAGCTTGAGAAGAAATCAAA+GGG + chr4_1:77270261-77270280 None:intergenic 30.0%
AAAGCTTGAGAAGAAATCAA+AGG + chr4_1:77270262-77270281 None:intergenic 30.0%
! CTGTCTAAAATTTACTGACT+TGG - chr4_1:77270282-77270301 Msa0573930:CDS 30.0%
! TTTTCGTGAGTTATGTCATA+TGG - chr4_1:77270428-77270447 Msa0573930:CDS 30.0%
TTTCGTGAGTTATGTCATAT+GGG - chr4_1:77270429-77270448 Msa0573930:CDS 30.0%
!!! GGAGCTTTTTCATTTTCATA+TGG + chr4_1:77270525-77270544 None:intergenic 30.0%
ATGAAAATGAAAAAGCTCCT+TGG - chr4_1:77270526-77270545 Msa0573930:CDS 30.0%
TTTCAATAATGCTGTTATGG+TGG - chr4_1:77270560-77270579 Msa0573930:CDS 30.0%
ATTCCCATTATTGAAGATGT+AGG - chr4_1:77270603-77270622 Msa0573930:CDS 30.0%
CATTCCTACATCTTCAATAA+TGG + chr4_1:77270610-77270629 None:intergenic 30.0%
! AAGAGTAGTTTAGTTTCTGA+TGG - chr4_1:77270642-77270661 Msa0573930:CDS 30.0%
TTACTTGAGAAGAAAGATGT+TGG - chr4_1:77270705-77270724 Msa0573930:CDS 30.0%
! AACTGCTATGAAACTTTTCT+TGG + chr4_1:77270766-77270785 None:intergenic 30.0%
! GAAAAGTTTCATAGCAGTTT+TGG - chr4_1:77270767-77270786 Msa0573930:CDS 30.0%
! GTTCAATTTCATTGACTGAT+CGG - chr4_1:77270928-77270947 Msa0573930:intron 30.0%
ATATTACTGTGAGAAAGATG+AGG - chr4_1:77271018-77271037 Msa0573930:intron 30.0%
TATCAACATTCTTGACTTAG+GGG + chr4_1:77271054-77271073 None:intergenic 30.0%
GTATCAACATTCTTGACTTA+GGG + chr4_1:77271055-77271074 None:intergenic 30.0%
TGTATCAACATTCTTGACTT+AGG + chr4_1:77271056-77271075 None:intergenic 30.0%
TAAGTCAAGAATGTTGATAC+AGG - chr4_1:77271055-77271074 Msa0573930:intron 30.0%
! TACCTGAAAAATTGTTGTTG+TGG - chr4_1:77271160-77271179 Msa0573930:intron 30.0%
TGCAATCAAAGTAGAATTTC+AGG + chr4_1:77271202-77271221 None:intergenic 30.0%
GCAATCATTCTATGTTCAAA+AGG - chr4_1:77271332-77271351 Msa0573930:intron 30.0%
AACTAAAGTAAGTTCTACTC+TGG - chr4_1:77271391-77271410 Msa0573930:intron 30.0%
TCTTTCCTAATTGTATGTGT+AGG - chr4_1:77271452-77271471 Msa0573930:intron 30.0%
!! AGTATAGGTTGATGAAAGAT+CGG + chr4_1:77271535-77271554 None:intergenic 30.0%
CTTTCATCAACCTATACTTA+AGG - chr4_1:77271537-77271556 Msa0573930:intron 30.0%
TTCATCAACCTATACTTAAG+GGG - chr4_1:77271539-77271558 Msa0573930:intron 30.0%
AACTAAATGGCATATAGAAC+AGG + chr4_1:77271645-77271664 None:intergenic 30.0%
TCTAACATACGTGAACTAAA+TGG + chr4_1:77271658-77271677 None:intergenic 30.0%
ATGAGAGTTACGTACATAAA+CGG + chr4_1:77271686-77271705 None:intergenic 30.0%
ATGTACGTAACTCTCATAAT+CGG - chr4_1:77271689-77271708 Msa0573930:CDS 30.0%
ATCATGCAAGTGGAATTAAA+GGG + chr4_1:77271853-77271872 None:intergenic 30.0%
! AGCGTAACAACCTTATTTAT+TGG - chr4_1:77271991-77272010 Msa0573930:intron 30.0%
CCTTTCTATTATTCAAGCTT+AGG + chr4_1:77272248-77272267 None:intergenic 30.0%
CCTAAGCTTGAATAATAGAA+AGG - chr4_1:77272245-77272264 Msa0573930:intron 30.0%
GGATATTTGATTATGGAGTT+CGG + chr4_1:77272312-77272331 None:intergenic 30.0%
AAAAATGCAAACTGAGAACA+CGG + chr4_1:77272334-77272353 None:intergenic 30.0%
! AGTTTTACTTCTCATGTTGT+AGG - chr4_1:77272503-77272522 Msa0573930:CDS 30.0%
! CTTTTATGCAGTATTCCATA+TGG + chr4_1:77272548-77272567 None:intergenic 30.0%
!! GTAACTCAAGGCTTTAAAAT+AGG - chr4_1:77272585-77272604 Msa0573930:CDS 30.0%
TTCCAATGCTTTAGCAATAT+CGG + chr4_1:77272741-77272760 None:intergenic 30.0%
TATTAACTTCATACCCTTTG+GGG + chr4_1:77272866-77272885 None:intergenic 30.0%
CTATTAACTTCATACCCTTT+GGG + chr4_1:77272867-77272886 None:intergenic 30.0%
AAATTGTCAGATATGATCAC+AGG + chr4_1:77272927-77272946 None:intergenic 30.0%
! TTTAAAACCTCGAAAAATGG+CGG + chr4_1:77272954-77272973 None:intergenic 30.0%
! GAGTTCAGAAACATTTTCTT+TGG - chr4_1:77273084-77273103 Msa0573930:five_prime_UTR 30.0%
GAAAGAATATGACAAGAACT+AGG - chr4_1:77273319-77273338 Msa0573930:five_prime_UTR 30.0%
TTCAGTACTAGTAACAAACT+AGG + chr4_1:77273361-77273380 None:intergenic 30.0%
TCAATGTACTTAACAGTTAC+AGG - chr4_1:77273411-77273430 Msa0573930:five_prime_UTR 30.0%
TGAAGGCAAGAAAACAGAAA+GGG + chr4_1:77270175-77270194 None:intergenic 35.0%
ATGAAGGCAAGAAAACAGAA+AGG + chr4_1:77270176-77270195 None:intergenic 35.0%
TCATACATGCAACTGAGTAT+TGG - chr4_1:77270309-77270328 Msa0573930:CDS 35.0%
CATACATGCAACTGAGTATT+GGG - chr4_1:77270310-77270329 Msa0573930:CDS 35.0%
! GTTAACTTGGCAAAGAACTA+AGG + chr4_1:77270397-77270416 None:intergenic 35.0%
TTGAAATCAGAAGAAGACCA+AGG + chr4_1:77270546-77270565 None:intergenic 35.0%
AGCTTGGAGTTAGATAATGT+AGG - chr4_1:77270738-77270757 Msa0573930:CDS 35.0%
! CTTTTCAGTGAATCCATCTT+TGG - chr4_1:77270809-77270828 Msa0573930:CDS 35.0%
CAATGAAATTGAACTCTGTG+AGG + chr4_1:77270922-77270941 None:intergenic 35.0%
ATCAACATTCTTGACTTAGG+GGG + chr4_1:77271053-77271072 None:intergenic 35.0%
! CTCCACAACAACAATTTTTC+AGG + chr4_1:77271165-77271184 None:intergenic 35.0%
TACTTTGATTGCAGAAACCA+AGG - chr4_1:77271209-77271228 Msa0573930:intron 35.0%
GTGGAAATCCAAACTTGATA+TGG - chr4_1:77271231-77271250 Msa0573930:intron 35.0%
ATATGGAAATTCTTGCAGCA+AGG - chr4_1:77271248-77271267 Msa0573930:intron 35.0%
TCCTACAGTAACAAATGACA+AGG - chr4_1:77271288-77271307 Msa0573930:intron 35.0%
! TCCTTGTCATTTGTTACTGT+AGG + chr4_1:77271292-77271311 None:intergenic 35.0%
ATAGAATGATTGCGAAAGAG+GGG + chr4_1:77271325-77271344 None:intergenic 35.0%
CATAGAATGATTGCGAAAGA+GGG + chr4_1:77271326-77271345 None:intergenic 35.0%
ACATAGAATGATTGCGAAAG+AGG + chr4_1:77271327-77271346 None:intergenic 35.0%
TAGCTCCTACACATACAATT+AGG + chr4_1:77271460-77271479 None:intergenic 35.0%
GTGGTTTATGAAATTGATGG+AGG - chr4_1:77271579-77271598 Msa0573930:CDS 35.0%
AAATGGCATATAGAACAGGT+GGG + chr4_1:77271641-77271660 None:intergenic 35.0%
TAAATGGCATATAGAACAGG+TGG + chr4_1:77271642-77271661 None:intergenic 35.0%
AAATCTCCGTCAGTATTTGA+AGG - chr4_1:77271714-77271733 Msa0573930:CDS 35.0%
TGTAGTCCTTCAAATACTGA+CGG + chr4_1:77271723-77271742 None:intergenic 35.0%
CATTACTTTAACAGAACTGG+CGG - chr4_1:77271766-77271785 Msa0573930:CDS 35.0%
CATCATGCAAGTGGAATTAA+AGG + chr4_1:77271854-77271873 None:intergenic 35.0%
! ACAAAGTATTTGTTGGGGTA+TGG - chr4_1:77272027-77272046 Msa0573930:CDS 35.0%
! ATGGCTCATAATTTAGGACT+CGG - chr4_1:77272046-77272065 Msa0573930:CDS 35.0%
GAGAGATAAAGTAGTTGTAG+AGG + chr4_1:77272074-77272093 None:intergenic 35.0%
! TTTTATACAGGGACAGTTCA+CGG - chr4_1:77272169-77272188 Msa0573930:intron 35.0%
TCACGGTTAATCTTGTAAAC+TGG - chr4_1:77272186-77272205 Msa0573930:intron 35.0%
GAACACGGGATATTTGATTA+TGG + chr4_1:77272319-77272338 None:intergenic 35.0%
AAAATGCAAACTGAGAACAC+GGG + chr4_1:77272333-77272352 None:intergenic 35.0%
!! ATATGGTGCCTTGTTTCATA+TGG + chr4_1:77272531-77272550 None:intergenic 35.0%
! ATATGAAACAAGGCACCATA+TGG - chr4_1:77272530-77272549 Msa0573930:CDS 35.0%
AGTTACATCAGAATTGCAGA+GGG + chr4_1:77272571-77272590 None:intergenic 35.0%
TGCAATTCTGATGTAACTCA+AGG - chr4_1:77272573-77272592 Msa0573930:CDS 35.0%
!! GATATTGCTAAAGCATTGGA+AGG - chr4_1:77272740-77272759 Msa0573930:CDS 35.0%
GACCCTGATATGAAGATATT+TGG - chr4_1:77272768-77272787 Msa0573930:CDS 35.0%
GGCCAAATATCTTCATATCA+GGG + chr4_1:77272773-77272792 None:intergenic 35.0%
GCTATTAACTTCATACCCTT+TGG + chr4_1:77272868-77272887 None:intergenic 35.0%
TTGGAATATCTTTCTCTGCA+AGG + chr4_1:77272998-77273017 None:intergenic 35.0%
AAGTATCTCAAGCTACAACT+TGG - chr4_1:77273053-77273072 Msa0573930:five_prime_UTR 35.0%
AATCTGCTTTGTGGGTTTAG+TGG - chr4_1:77269966-77269985 Msa0573930:three_prime_UTR 40.0%
TTGCGATCTTCAACTTTCTC+AGG + chr4_1:77270006-77270025 None:intergenic 40.0%
GAAGATCGCAAAACAGAAAG+AGG - chr4_1:77270015-77270034 Msa0573930:three_prime_UTR 40.0%
! TCTGATGGTGTTTCTGAACA+TGG - chr4_1:77270657-77270676 Msa0573930:CDS 40.0%
!! TTTCATAGCAGTTTTGGCCA+AGG - chr4_1:77270773-77270792 Msa0573930:CDS 40.0%
AAAGTCAACCTTACTCTCCT+TGG + chr4_1:77270793-77270812 None:intergenic 40.0%
! ATCCATCTTTGGAGACGAAA+AGG - chr4_1:77270820-77270839 Msa0573930:CDS 40.0%
! TCCATCTTTGGAGACGAAAA+GGG - chr4_1:77270821-77270840 Msa0573930:CDS 40.0%
! ATTGATTCAAGGGAGCATGA+TGG - chr4_1:77270882-77270901 Msa0573930:intron 40.0%
GCAGACAACTTCTTAATCTG+AGG + chr4_1:77270972-77270991 None:intergenic 40.0%
!! TGCCTCAGATAAAAGTGTTG+CGG - chr4_1:77270989-77271008 Msa0573930:intron 40.0%
TTTGATTGCAGAAACCAAGG+TGG - chr4_1:77271212-77271231 Msa0573930:intron 40.0%
TCAAGTTTGGATTTCCACCT+TGG + chr4_1:77271229-77271248 None:intergenic 40.0%
GCATATAGCCCCTTAAGTAT+AGG + chr4_1:77271550-77271569 None:intergenic 40.0%
GGGGTGGTTTATGAAATTGA+TGG - chr4_1:77271576-77271595 Msa0573930:CDS 40.0%
CATGGCAAGCAACAAGAAAA+TGG + chr4_1:77271798-77271817 None:intergenic 40.0%
! TTCTTGTTGCTTGCCATGAT+TGG - chr4_1:77271800-77271819 Msa0573930:CDS 40.0%
! TCTTGTTGCTTGCCATGATT+GGG - chr4_1:77271801-77271820 Msa0573930:CDS 40.0%
ATCTTGGTTCATCATGCAAG+TGG + chr4_1:77271863-77271882 None:intergenic 40.0%
CTTATCGACCCTCATCAAAA+AGG + chr4_1:77271973-77271992 None:intergenic 40.0%
TGGGGTATGGCTCATAATTT+AGG - chr4_1:77272040-77272059 Msa0573930:CDS 40.0%
TGTAGGCTCCATATGAAACA+AGG - chr4_1:77272520-77272539 Msa0573930:CDS 40.0%
GAGTTACATCAGAATTGCAG+AGG + chr4_1:77272572-77272591 None:intergenic 40.0%
TGTTTCTCTCCCTGAAACTA+TGG - chr4_1:77272614-77272633 Msa0573930:CDS 40.0%
TTCTATGCTGGAAACATGCA+CGG - chr4_1:77272705-77272724 Msa0573930:CDS 40.0%
GTCCGATATTGCTAAAGCAT+TGG - chr4_1:77272736-77272755 Msa0573930:CDS 40.0%
GGGCCAAATATCTTCATATC+AGG + chr4_1:77272774-77272793 None:intergenic 40.0%
AAATTCTGCATATGCCCCAA+AGG - chr4_1:77272849-77272868 Msa0573930:CDS 40.0%
AATTCTGCATATGCCCCAAA+GGG - chr4_1:77272850-77272869 Msa0573930:CDS 40.0%
GGTATGAAGTTAATAGCCCT+CGG - chr4_1:77272871-77272890 Msa0573930:CDS 40.0%
GTATGAAGTTAATAGCCCTC+GGG - chr4_1:77272872-77272891 Msa0573930:CDS 40.0%
AGGTACGCACTCGTAAAATA+TGG + chr4_1:77272907-77272926 None:intergenic 40.0%
AGCTTGAGATACTTCTCTTC+AGG + chr4_1:77273047-77273066 None:intergenic 40.0%
TGAAAGAGGTCGTACTTCAA+AGG + chr4_1:77273119-77273138 None:intergenic 40.0%
ACATGACCCTTCACTCAATT+TGG - chr4_1:77273138-77273157 Msa0573930:intron 40.0%
!! CTTCTGCTTTCTAGTTCAGA+GGG + chr4_1:77273210-77273229 None:intergenic 40.0%
! TGCTGTTATGGTGGAGAAAG+TGG - chr4_1:77270569-77270588 Msa0573930:CDS 45.0%
! TGGTGTTTCTGAACATGGAG+TGG - chr4_1:77270662-77270681 Msa0573930:CDS 45.0%
!! AGTTTTGGCCAAGGAGAGTA+AGG - chr4_1:77270782-77270801 Msa0573930:CDS 45.0%
! CCATCTTTGGAGACGAAAAG+GGG - chr4_1:77270822-77270841 Msa0573930:CDS 45.0%
!! GAGCATGATGGAGTTGGTTT+TGG - chr4_1:77270894-77270913 Msa0573930:intron 45.0%
AGTTCTACTCTGGCATGCAT+TGG - chr4_1:77271401-77271420 Msa0573930:intron 45.0%
GTGTAGGAGCTATGAACTGA+TGG - chr4_1:77271468-77271487 Msa0573930:intron 45.0%
ACTTAAGGGGCTATATGCGT+CGG - chr4_1:77271552-77271571 Msa0573930:intron 45.0%
TGTTGTGAAGGATCCTGCAA+AGG - chr4_1:77271615-77271634 Msa0573930:CDS 45.0%
GCGAACATCATACCCAATCA+TGG + chr4_1:77271816-77271835 None:intergenic 45.0%
AAAAAGGCCAGGACAATGCT+AGG + chr4_1:77271957-77271976 None:intergenic 45.0%
!! TTGTCCTGGCCTTTTTGATG+AGG - chr4_1:77271961-77271980 Msa0573930:intron 45.0%
!! TGTCCTGGCCTTTTTGATGA+GGG - chr4_1:77271962-77271981 Msa0573930:intron 45.0%
AAGCAACTCAGCAGAATACC+TGG + chr4_1:77272382-77272401 None:intergenic 45.0%
CGAAATCCTCAGAGAGATCT+AGG - chr4_1:77272648-77272667 Msa0573930:CDS 45.0%
AATCCTCAGAGAGATCTAGG+CGG - chr4_1:77272651-77272670 Msa0573930:CDS 45.0%
! AGAATGGACCTTTGTTGTGG+AGG + chr4_1:77272681-77272700 None:intergenic 45.0%
TCCACAACAAAGGTCCATTC+TGG - chr4_1:77272680-77272699 Msa0573930:CDS 45.0%
GTGCATGTTTCCAGCATAGA+AGG + chr4_1:77272706-77272725 None:intergenic 45.0%
ATATTTGGCCCAATGCCTCA+TGG - chr4_1:77272783-77272802 Msa0573930:CDS 45.0%
! ATTTTGTGTGCAACGCCATG+AGG + chr4_1:77272801-77272820 None:intergenic 45.0%
ATATGGCTTCAACTACCCGA+GGG + chr4_1:77272890-77272909 None:intergenic 45.0%
AATATGGCTTCAACTACCCG+AGG + chr4_1:77272891-77272910 None:intergenic 45.0%
!!! TTTTGTGCCGCCATTTTTCG+AGG - chr4_1:77272944-77272963 Msa0573930:CDS 45.0%
AGTGAAGGGTCATGTGAAAG+AGG + chr4_1:77273133-77273152 None:intergenic 45.0%
!! GCTTCTGCTTTCTAGTTCAG+AGG + chr4_1:77273211-77273230 None:intergenic 45.0%
! CTAGAAAGCAGAAGCACATC+TGG - chr4_1:77273216-77273235 Msa0573930:intron 45.0%
CACTGCTTCTTAAGCTGTAG+AGG - chr4_1:77273282-77273301 Msa0573930:five_prime_UTR 45.0%
CTCGAGTCTTCACTACACTG+AGG - chr4_1:77270083-77270102 Msa0573930:three_prime_UTR 50.0%
! CCCCTTTTCGTCTCCAAAGA+TGG + chr4_1:77270825-77270844 None:intergenic 50.0%
! TCAAGGGAGCATGATGGAGT+TGG - chr4_1:77270888-77270907 Msa0573930:intron 50.0%
! CGCCGCAACACTTTTATCTG+AGG + chr4_1:77270994-77271013 None:intergenic 50.0%
CCATTGCACGAGAAGAAGTG+CGG + chr4_1:77271106-77271125 None:intergenic 50.0%
GTCCTTACCTAGCATTGTCC+TGG - chr4_1:77271947-77271966 Msa0573930:intron 50.0%
CTACATCTCTGACTGCAGAG+CGG + chr4_1:77272279-77272298 None:intergenic 50.0%
CTCTCCCTGAAACTATGGTC+CGG - chr4_1:77272619-77272638 Msa0573930:CDS 50.0%
! TCTGAGGATTTCGTACCGAC+CGG + chr4_1:77272641-77272660 None:intergenic 50.0%
TTTCCGCCTAGATCTCTCTG+AGG + chr4_1:77272657-77272676 None:intergenic 50.0%
GCGGAAAACCTCCACAACAA+AGG - chr4_1:77272670-77272689 Msa0573930:CDS 50.0%
GCCAGAATGGACCTTTGTTG+TGG + chr4_1:77272684-77272703 None:intergenic 50.0%
! TCCATTCTGGCCTTCTATGC+TGG - chr4_1:77272693-77272712 Msa0573930:CDS 50.0%
TCCAGCATAGAAGGCCAGAA+TGG + chr4_1:77272697-77272716 None:intergenic 50.0%
! TTGCACGAGAAGAAGTGCGG+CGG + chr4_1:77271103-77271122 None:intergenic 55.0%
CCGCACTTCTTCTCGTGCAA+TGG - chr4_1:77271103-77271122 Msa0573930:intron 55.0%
TAAGGGGCTATATGCGTCGG+AGG - chr4_1:77271555-77271574 Msa0573930:intron 55.0%
AAGGGGCTATATGCGTCGGA+GGG - chr4_1:77271556-77271575 Msa0573930:intron 55.0%
TAGAACAGGTGGGCCTTTGC+AGG + chr4_1:77271631-77271650 None:intergenic 55.0%
GGCCAGGACAATGCTAGGTA+AGG + chr4_1:77271952-77271971 None:intergenic 55.0%
CGACCCTCATCAAAAAGGCC+AGG + chr4_1:77271968-77271987 None:intergenic 55.0%
CGCTCTGCAGTCAGAGATGT+AGG - chr4_1:77272277-77272296 Msa0573930:CDS 55.0%
CCGACCGGACCATAGTTTCA+GGG + chr4_1:77272626-77272645 None:intergenic 55.0%
CCCTGAAACTATGGTCCGGT+CGG - chr4_1:77272623-77272642 Msa0573930:CDS 55.0%
ACCGACCGGACCATAGTTTC+AGG + chr4_1:77272627-77272646 None:intergenic 55.0%
! GTGCAACGCCATGAGGCATT+GGG + chr4_1:77272794-77272813 None:intergenic 55.0%
TGTGCAACGCCATGAGGCAT+TGG + chr4_1:77272795-77272814 None:intergenic 55.0%
AGGGGCTATATGCGTCGGAG+GGG - chr4_1:77271557-77271576 Msa0573930:intron 60.0%
TGCGGCGGCGCTCTAGCAAA+TGG + chr4_1:77271088-77271107 None:intergenic 65.0%
GGCTATATGCGTCGGAGGGG+TGG - chr4_1:77271560-77271579 Msa0573930:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 77269917 77273463 77269917 ID=Msa0573930;Name=Msa0573930
chr4_1 mRNA 77269917 77273463 77269917 ID=Msa0573930-mRNA-1;Parent=Msa0573930;Name=Msa0573930-mRNA-1;_AED=0.06;_eAED=0.06;_QI=349|1|1|1|0.75|0.6|5|264|620
chr4_1 exon 77269917 77270855 77269917 ID=Msa0573930-mRNA-1:exon:27190;Parent=Msa0573930-mRNA-1
chr4_1 exon 77271558 77271906 77271558 ID=Msa0573930-mRNA-1:exon:27189;Parent=Msa0573930-mRNA-1
chr4_1 exon 77272027 77272157 77272027 ID=Msa0573930-mRNA-1:exon:27188;Parent=Msa0573930-mRNA-1
chr4_1 exon 77272256 77273124 77272256 ID=Msa0573930-mRNA-1:exon:27187;Parent=Msa0573930-mRNA-1
chr4_1 exon 77273276 77273463 77273276 ID=Msa0573930-mRNA-1:exon:27186;Parent=Msa0573930-mRNA-1
chr4_1 five_prime_UTR 77273276 77273463 77273276 ID=Msa0573930-mRNA-1:five_prime_utr;Parent=Msa0573930-mRNA-1
chr4_1 five_prime_UTR 77272964 77273124 77272964 ID=Msa0573930-mRNA-1:five_prime_utr;Parent=Msa0573930-mRNA-1
chr4_1 CDS 77272256 77272963 77272256 ID=Msa0573930-mRNA-1:cds;Parent=Msa0573930-mRNA-1
chr4_1 CDS 77272027 77272157 77272027 ID=Msa0573930-mRNA-1:cds;Parent=Msa0573930-mRNA-1
chr4_1 CDS 77271558 77271906 77271558 ID=Msa0573930-mRNA-1:cds;Parent=Msa0573930-mRNA-1
chr4_1 CDS 77270181 77270855 77270181 ID=Msa0573930-mRNA-1:cds;Parent=Msa0573930-mRNA-1
chr4_1 three_prime_UTR 77269917 77270180 77269917 ID=Msa0573930-mRNA-1:three_prime_utr;Parent=Msa0573930-mRNA-1
Gene Sequence

>Msa0573930

ATGTTTCAATATTTTCGTGAGTTATGTCATATGGGAAACCAAAGATTACTTTTTTTGTTGGGAGCACTTGCTGTTAATTATTTACTGTTTCAATCAATTTTGGTTCCATATGAAAATGAAAAAGCTCCTTGGTCTTCTTCTGATTTCAATAATGCTGTTATGGTGGAGAAAGTGGAGAAAGTGAATATTCCCATTATTGAAGATGTAGGAATGCATAACTATGCAAAGAGTAGTTTAGTTTCTGATGGTGTTTCTGAACATGGAGTGGATAGAAATGATTTTCATATTTTACTTGAGAAGAAAGATGTTGGTAAAAATAAAAGCTTGGAGTTAGATAATGTAGGTTCCAAGAAAAGTTTCATAGCAGTTTTGGCCAAGGAGAGTAAGGTTGACTTTTCAGTGAATCCATCTTTGGAGACGAAAAGGGGAATTTCTACAATTTCTCAGTTGGTAAAAAGTAAAAATATTGATTCAAGGGAGCATGATGGAGTTGGTTTTGGTGCCTCACAGAGTTCAATTTCATTGACTGATCGGACTCGTCTTGAAAGTTCTCCTCAGATTAAGAAGTTGTCTGCCTCAGATAAAAGTGTTGCGGCGAATAATATTACTGTGAGAAAGATGAGGTGTAATATGCCCCCTAAGTCAAGAATGTTGATACAGGAGATGAACCATTTGCTAGAGCGCCGCCGCACTTCTTCTCGTGCAATGAAACCAAGGTGGAAATCCAAACTTGATATGGAAATTCTTGCAGCAAGGTCAGAGATTGAGCATGCTCCTACAGTAACAAATGACAAGGAACTCTATGCACCCCTCTTTCGCAATCATTCTATGTTCAAAAGGAGCTATGAACTGATGGAACACACACTCAAAGTATACATATACATGGAAGGAAATAAACCGATCTTTCATCAACCTATACTTAAGGGGCTATATGCGTCGGAGGGGTGGTTTATGAAATTGATGGAGGAAAATAAGCAATTTGTTGTGAAGGATCCTGCAAAGGCCCACCTGTTCTATATGCCATTTAGTTCACGTATGTTAGAGTTCTCCGTTTATGTACGTAACTCTCATAATCGGACAAATCTCCGTCAGTATTTGAAGGACTACACAGATAAAATTTCAGCGAAGTATCATTACTTTAACAGAACTGGCGGTGCTGACCATTTTCTTGTTGCTTGCCATGATTGGGCTCCATATGAAACAAGGCACCATATGGAATACTGCATAAAAGCCCTCTGCAATTCTGATGTAACTCAAGGCTTTAAAATAGGAAGAGATGTTTCTCTCCCTGAAACTATGGTCCGGTCGGTACGAAATCCTCAGAGAGATCTAGGCGGAAAACCTCCACAACAAAGGTCCATTCTGGCCTTCTATGCTGGAAACATGCACGGTTATTTGCGTCCGATATTGCTAAAGCATTGGAAGGACAAAGACCCTGATATGAAGATATTTGGCCCAATGCCTCATGGCGTTGCACACAAAATGAATTACATCGAACACATGAAGAATAGCAAATTCTGCATATGCCCCAAAGGGTATGAAGTTAATAGCCCTCGGGTAGTTGAAGCCATATTTTACGAGTGCGTACCTGTGATCATATCTGACAATTTTGTGCCGCCATTTTTCGAGGTTTTAAATTGGGATGCATTCTCATTAATCCTTGCAGAGAAAGATATTCCAAACTTGAAACAAATACTACTTTCAGTGCCTGAAGAGAAGTATCTCAAGCTACAACTTGGTGTCAAAAGAGTTCAGAAACATTTTCTTTGGCATACTAAACCTTTGAAGTACGACCTCTTTCACATGACCCTTCACTCAATTTGGTATAATAGAGTGTTTCAAATAAATGTGAGAAAAAAATGA
Protein sequence

>Msa0573930

MFQYFRELCHMGNQRLLFLLGALAVNYLLFQSILVPYENEKAPWSSSDFNNAVMVEKVEKVNIPIIEDVGMHNYAKSSLVSDGVSEHGVDRNDFHILLEKKDVGKNKSLELDNVGSKKSFIAVLAKESKVDFSVNPSLETKRGISTISQLVKSKNIDSREHDGVGFGASQSSISLTDRTRLESSPQIKKLSASDKSVAANNITVRKMRCNMPPKSRMLIQEMNHLLERRRTSSRAMKPRWKSKLDMEILAARSEIEHAPTVTNDKELYAPLFRNHSMFKRSYELMEHTLKVYIYMEGNKPIFHQPILKGLYASEGWFMKLMEENKQFVVKDPAKAHLFYMPFSSRMLEFSVYVRNSHNRTNLRQYLKDYTDKISAKYHYFNRTGGADHFLVACHDWAPYETRHHMEYCIKALCNSDVTQGFKIGRDVSLPETMVRSVRNPQRDLGGKPPQQRSILAFYAGNMHGYLRPILLKHWKDKDPDMKIFGPMPHGVAHKMNYIEHMKNSKFCICPKGYEVNSPRVVEAIFYECVPVIISDNFVPPFFEVLNWDAFSLILAEKDIPNLKQILLSVPEEKYLKLQLGVKRVQKHFLWHTKPLKYDLFHMTLHSIWYNRVFQINVRKK*