Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0577720 | AET04964.2 | 91.176 | 68 | 6 | 0 | 3 | 70 | 42 | 109 | 6.18e-34 | 122 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0577720 | G7LHA1 | 91.176 | 68 | 6 | 0 | 3 | 70 | 42 | 109 | 2.95e-34 | 122 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0577620 | Msa0577720 | 0.856609 | 2.865975e-62 | -8.615850e-47 |
| Msa0577630 | Msa0577720 | 0.949288 | 1.722028e-107 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0577720 | MtrunA17_Chr8g0377541 | 91.176 | 68 | 6 | 0 | 3 | 70 | 13 | 80 | 9.61e-38 | 122 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTCAGTATGACTGAACTTA+TGG | 0.282326 | 4_1:+82045940 | Msa0577720:CDS |
| CACACTGAAATTGCAGATAC+CGG | 0.472140 | 4_1:+82045774 | Msa0577720:CDS |
| CGGATTTGCGAAAGTGCAGC+AGG | 0.490310 | 4_1:-82045980 | None:intergenic |
| GTAAACAAACCTTTGATGAT+CGG | 0.498652 | 4_1:-82046000 | None:intergenic |
| GAAATTGCAGATACCGGGTT+GGG | 0.520567 | 4_1:+82045780 | Msa0577720:CDS |
| TTCGCAAATCCGATCATCAA+AGG | 0.535981 | 4_1:+82045991 | Msa0577720:CDS |
| AGAAAACGCTTCACCCAACC+CGG | 0.576346 | 4_1:-82045793 | None:intergenic |
| TGTTGATGTTAAAGGCACTT+TGG | 0.580507 | 4_1:+82045899 | Msa0577720:intron |
| TGAAATTGCAGATACCGGGT+TGG | 0.590918 | 4_1:+82045779 | Msa0577720:CDS |
| ACACTGAAATTGCAGATACC+GGG | 0.596769 | 4_1:+82045775 | Msa0577720:CDS |
| CGGTATCTGCAATTTCAGTG+TGG | 0.687289 | 4_1:-82045773 | None:intergenic |
| CCATAAGTTCAGTCATACTG+AGG | 0.687637 | 4_1:-82045940 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATAAGTTGTTGATGTTAA+AGG | + | chr4_1:82045891-82045910 | Msa0577720:intron | 20.0% |
| ! | CGTTTTCTGAATTTGTCAAA+AGG | + | chr4_1:82045808-82045827 | Msa0577720:CDS | 30.0% |
| ! | GTAAACAAACCTTTGATGAT+CGG | - | chr4_1:82046003-82046022 | None:intergenic | 30.0% |
| !! | TGTTGATGTTAAAGGCACTT+TGG | + | chr4_1:82045899-82045918 | Msa0577720:intron | 35.0% |
| CACACTGAAATTGCAGATAC+CGG | + | chr4_1:82045774-82045793 | Msa0577720:CDS | 40.0% | |
| ACACTGAAATTGCAGATACC+GGG | + | chr4_1:82045775-82045794 | Msa0577720:CDS | 40.0% | |
| TTGCACGAAGTTGAATGCAA+AGG | - | chr4_1:82045843-82045862 | None:intergenic | 40.0% | |
| AACTTCGTGCAACTGTGTAA+AGG | + | chr4_1:82045851-82045870 | Msa0577720:intron | 40.0% | |
| CAACTGTGTAAAGGTTTGAG+TGG | + | chr4_1:82045860-82045879 | Msa0577720:intron | 40.0% | |
| CCATAAGTTCAGTCATACTG+AGG | - | chr4_1:82045943-82045962 | None:intergenic | 40.0% | |
| CCTCAGTATGACTGAACTTA+TGG | + | chr4_1:82045940-82045959 | Msa0577720:CDS | 40.0% | |
| TTCGCAAATCCGATCATCAA+AGG | + | chr4_1:82045991-82046010 | Msa0577720:CDS | 40.0% | |
| CGGTATCTGCAATTTCAGTG+TGG | - | chr4_1:82045776-82045795 | None:intergenic | 45.0% | |
| TGAAATTGCAGATACCGGGT+TGG | + | chr4_1:82045779-82045798 | Msa0577720:CDS | 45.0% | |
| GAAATTGCAGATACCGGGTT+GGG | + | chr4_1:82045780-82045799 | Msa0577720:CDS | 45.0% | |
| AGAAAACGCTTCACCCAACC+CGG | - | chr4_1:82045796-82045815 | None:intergenic | 50.0% | |
| ! | CGGATTTGCGAAAGTGCAGC+AGG | - | chr4_1:82045983-82046002 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 82045756 | 82046054 | 82045756 | ID=Msa0577720;Name=Msa0577720 |
| chr4_1 | mRNA | 82045756 | 82046054 | 82045756 | ID=Msa0577720-mRNA-1;Parent=Msa0577720;Name=Msa0577720-mRNA-1;_AED=0.24;_eAED=0.24;_QI=0|0|0|1|1|1|2|0|71 |
| chr4_1 | exon | 82045756 | 82045829 | 82045756 | ID=Msa0577720-mRNA-1:exon:29635;Parent=Msa0577720-mRNA-1 |
| chr4_1 | exon | 82045913 | 82046054 | 82045913 | ID=Msa0577720-mRNA-1:exon:29636;Parent=Msa0577720-mRNA-1 |
| chr4_1 | CDS | 82045756 | 82045829 | 82045756 | ID=Msa0577720-mRNA-1:cds;Parent=Msa0577720-mRNA-1 |
| chr4_1 | CDS | 82045913 | 82046054 | 82045913 | ID=Msa0577720-mRNA-1:cds;Parent=Msa0577720-mRNA-1 |
| Gene Sequence |
| Protein sequence |
