Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0581120 | KEH19403.1 | 71.429 | 133 | 34 | 2 | 11 | 143 | 18 | 146 | 3.77e-59 | 191 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0581120 | sp|Q9FNG6|C3H51_ARATH | 50.000 | 160 | 30 | 1 | 18 | 127 | 129 | 288 | 9.83e-45 | 153 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0581120 | A0A072U5J1 | 71.429 | 133 | 34 | 2 | 11 | 143 | 18 | 146 | 1.80e-59 | 191 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0581120 | TF | C3H |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0581120 | Msa1126840 | 0.948506 | 8.245896e-107 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0581120 | MtrunA17_Chr7g0269781 | 51.309 | 191 | 25 | 3 | 1 | 124 | 51 | 240 | 3.84e-55 | 176 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0581120 | AT5G06420.1 | 50.000 | 160 | 30 | 1 | 18 | 127 | 129 | 288 | 9.99e-46 | 153 |
| Msa0581120 | AT5G06420.2 | 50.000 | 160 | 30 | 1 | 18 | 127 | 129 | 288 | 9.99e-46 | 153 |
| Msa0581120 | AT1G01350.1 | 49.375 | 160 | 31 | 1 | 18 | 127 | 94 | 253 | 1.15e-44 | 149 |
Find 29 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAAGGGAAAAGGACTTTC+TGG | 0.207237 | 4_1:+86462811 | Msa0581120:CDS |
| GCGCACACTCTTTGCTTTCT+CGG | 0.276894 | 4_1:-86462762 | None:intergenic |
| GACCGAGGCGACTACAATTC+CGG | 0.339363 | 4_1:+86462710 | Msa0581120:CDS |
| TGAAGACTCAAAATGAAAGT+TGG | 0.349810 | 4_1:-86462471 | None:intergenic |
| AAAATGAAAGTTGGGCTTCT+CGG | 0.382812 | 4_1:-86462462 | None:intergenic |
| TTGAAGTCTTTGCATATGTC+AGG | 0.387439 | 4_1:-86462641 | None:intergenic |
| GAAGACTCAAAATGAAAGTT+GGG | 0.391037 | 4_1:-86462470 | None:intergenic |
| TGACATATGCAAAGACTTCA+AGG | 0.411330 | 4_1:+86462643 | Msa0581120:CDS |
| ACCGAGGCGACTACAATTCC+GGG | 0.427387 | 4_1:+86462711 | Msa0581120:CDS |
| TAGCAAAGCAACAGCAACTT+TGG | 0.436558 | 4_1:+86462517 | Msa0581120:CDS |
| TGCAAAGACTTCAAGGAGAC+CGG | 0.477457 | 4_1:+86462650 | Msa0581120:CDS |
| GGTGGCAGTTGGACAAAGAA+TGG | 0.496757 | 4_1:+86462732 | Msa0581120:CDS |
| AAAGAGTGTGCGCGCGCTGA+TGG | 0.507062 | 4_1:+86462772 | Msa0581120:CDS |
| TTCAAGGAGACCGGGTATTG+TGG | 0.545327 | 4_1:+86462659 | Msa0581120:CDS |
| CTACAATTCCGGGTGGCAGT+TGG | 0.548426 | 4_1:+86462721 | Msa0581120:CDS |
| ACCCGGAATTGTAGTCGCCT+CGG | 0.550971 | 4_1:-86462712 | None:intergenic |
| CGCGCTGATGGTTTGTCTGA+AGG | 0.556877 | 4_1:+86462784 | Msa0581120:CDS |
| TTCTTTGTCCAACTGCCACC+CGG | 0.562902 | 4_1:-86462729 | None:intergenic |
| TGCAAGTTTCTTCACGACCG+AGG | 0.564969 | 4_1:+86462695 | Msa0581120:CDS |
| GAGACCGGGTATTGTGGCTA+CGG | 0.578795 | 4_1:+86462665 | Msa0581120:CDS |
| ATCGCCGTAGCCACAATACC+CGG | 0.586901 | 4_1:-86462669 | None:intergenic |
| GAAACTCTAATATGAGTCGA+AGG | 0.595574 | 4_1:-86462599 | None:intergenic |
| ACATGTAAATTACAAACACC+CGG | 0.625741 | 4_1:-86462852 | None:intergenic |
| GCAAAGACTTCAAGGAGACC+GGG | 0.633254 | 4_1:+86462651 | Msa0581120:CDS |
| ATCCGTGGGTCATACTCACA+CGG | 0.649413 | 4_1:+86462566 | Msa0581120:CDS |
| GTGTGAGTATGACCCACGGA+TGG | 0.652577 | 4_1:-86462564 | None:intergenic |
| GACCGTGTGAGTATGACCCA+CGG | 0.674066 | 4_1:-86462568 | None:intergenic |
| ATATGAGTCGAAGGTCTGAG+AGG | 0.697705 | 4_1:-86462590 | None:intergenic |
| GAGGCGACTACAATTCCGGG+TGG | 0.758896 | 4_1:+86462714 | Msa0581120:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGAGAAAAAGAACATGAAGA+AGG | + | chr4_1:86462421-86462440 | Msa0581120:CDS | 30.0% | |
| GAAGACTCAAAATGAAAGTT+GGG | - | chr4_1:86462473-86462492 | None:intergenic | 30.0% | |
| TGAAGACTCAAAATGAAAGT+TGG | - | chr4_1:86462474-86462493 | None:intergenic | 30.0% | |
| TTTGTCTGAAGGAAAAAAGA+AGG | + | chr4_1:86462795-86462814 | Msa0581120:CDS | 30.0% | |
| TTGTCTGAAGGAAAAAAGAA+GGG | + | chr4_1:86462796-86462815 | Msa0581120:CDS | 30.0% | |
| AAAATGAAAGTTGGGCTTCT+CGG | - | chr4_1:86462465-86462484 | None:intergenic | 35.0% | |
| !!! | ATTTTTCAAGTTCCATCCGT+GGG | + | chr4_1:86462552-86462571 | Msa0581120:CDS | 35.0% |
| GAAACTCTAATATGAGTCGA+AGG | - | chr4_1:86462602-86462621 | None:intergenic | 35.0% | |
| TTGAAGTCTTTGCATATGTC+AGG | - | chr4_1:86462644-86462663 | None:intergenic | 35.0% | |
| TGACATATGCAAAGACTTCA+AGG | + | chr4_1:86462643-86462662 | Msa0581120:CDS | 35.0% | |
| GAAGGAAAAAAGAAGGGAAA+AGG | + | chr4_1:86462802-86462821 | Msa0581120:CDS | 35.0% | |
| !! | GATTTTGCTGACTACAATTC+CGG | + | chr4_1:86462833-86462852 | Msa0581120:CDS | 35.0% |
| !! | ATTTTGCTGACTACAATTCC+GGG | + | chr4_1:86462834-86462853 | Msa0581120:CDS | 35.0% |
| TAGCAAAGCAACAGCAACTT+TGG | + | chr4_1:86462517-86462536 | Msa0581120:CDS | 40.0% | |
| !!! | CATTTTTCAAGTTCCATCCG+TGG | + | chr4_1:86462551-86462570 | Msa0581120:CDS | 40.0% |
| AAGAAGGGAAAAGGACTTTC+TGG | + | chr4_1:86462811-86462830 | Msa0581120:CDS | 40.0% | |
| ATATGAGTCGAAGGTCTGAG+AGG | - | chr4_1:86462593-86462612 | None:intergenic | 45.0% | |
| TGCAAAGACTTCAAGGAGAC+CGG | + | chr4_1:86462650-86462669 | Msa0581120:CDS | 45.0% | |
| ATCCGTGGGTCATACTCACA+CGG | + | chr4_1:86462566-86462585 | Msa0581120:CDS | 50.0% | |
| GCAAAGACTTCAAGGAGACC+GGG | + | chr4_1:86462651-86462670 | Msa0581120:CDS | 50.0% | |
| ! | TTCAAGGAGACCGGGTATTG+TGG | + | chr4_1:86462659-86462678 | Msa0581120:CDS | 50.0% |
| TGCAAGTTTCTTCACGACCG+AGG | + | chr4_1:86462695-86462714 | Msa0581120:CDS | 50.0% | |
| TTCTTTGTCCAACTGCCACC+CGG | - | chr4_1:86462732-86462751 | None:intergenic | 50.0% | |
| GGTGGCAGTTGGACAAAGAA+TGG | + | chr4_1:86462732-86462751 | Msa0581120:CDS | 50.0% | |
| GCGCACACTCTTTGCTTTCT+CGG | - | chr4_1:86462765-86462784 | None:intergenic | 50.0% | |
| GTGTGAGTATGACCCACGGA+TGG | - | chr4_1:86462567-86462586 | None:intergenic | 55.0% | |
| GACCGTGTGAGTATGACCCA+CGG | - | chr4_1:86462571-86462590 | None:intergenic | 55.0% | |
| ! | GAGACCGGGTATTGTGGCTA+CGG | + | chr4_1:86462665-86462684 | Msa0581120:CDS | 55.0% |
| ATCGCCGTAGCCACAATACC+CGG | - | chr4_1:86462672-86462691 | None:intergenic | 55.0% | |
| GACCGAGGCGACTACAATTC+CGG | + | chr4_1:86462710-86462729 | Msa0581120:CDS | 55.0% | |
| ACCGAGGCGACTACAATTCC+GGG | + | chr4_1:86462711-86462730 | Msa0581120:CDS | 55.0% | |
| ACCCGGAATTGTAGTCGCCT+CGG | - | chr4_1:86462715-86462734 | None:intergenic | 55.0% | |
| CTACAATTCCGGGTGGCAGT+TGG | + | chr4_1:86462721-86462740 | Msa0581120:CDS | 55.0% | |
| ! | CGCGCTGATGGTTTGTCTGA+AGG | + | chr4_1:86462784-86462803 | Msa0581120:CDS | 55.0% |
| GAGGCGACTACAATTCCGGG+TGG | + | chr4_1:86462714-86462733 | Msa0581120:CDS | 60.0% | |
| ! | AAAGAGTGTGCGCGCGCTGA+TGG | + | chr4_1:86462772-86462791 | Msa0581120:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 86462413 | 86462865 | 86462413 | ID=Msa0581120;Name=Msa0581120 |
| chr4_1 | mRNA | 86462413 | 86462865 | 86462413 | ID=Msa0581120-mRNA-1;Parent=Msa0581120;Name=Msa0581120-mRNA-1;_AED=0.45;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|150 |
| chr4_1 | exon | 86462413 | 86462865 | 86462413 | ID=Msa0581120-mRNA-1:exon:31951;Parent=Msa0581120-mRNA-1 |
| chr4_1 | CDS | 86462413 | 86462865 | 86462413 | ID=Msa0581120-mRNA-1:cds;Parent=Msa0581120-mRNA-1 |
| Gene Sequence |
| Protein sequence |
