Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1126840 | KEH19403.1 | 71.429 | 133 | 34 | 2 | 11 | 143 | 18 | 146 | 3.77e-59 | 191 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1126840 | sp|Q9FNG6|C3H51_ARATH | 50.000 | 160 | 30 | 1 | 18 | 127 | 129 | 288 | 9.83e-45 | 153 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1126840 | A0A072U5J1 | 71.429 | 133 | 34 | 2 | 11 | 143 | 18 | 146 | 1.80e-59 | 191 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1126840 | TF | C3H |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0581120 | Msa1126840 | 0.948506 | 8.245896e-107 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1126840 | MtrunA17_Chr7g0269781 | 51.309 | 191 | 25 | 3 | 1 | 124 | 51 | 240 | 3.84e-55 | 176 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1126840 | AT5G06420.1 | 50.000 | 160 | 30 | 1 | 18 | 127 | 129 | 288 | 9.99e-46 | 153 |
| Msa1126840 | AT5G06420.2 | 50.000 | 160 | 30 | 1 | 18 | 127 | 129 | 288 | 9.99e-46 | 153 |
| Msa1126840 | AT1G01350.1 | 49.375 | 160 | 31 | 1 | 18 | 127 | 94 | 253 | 1.15e-44 | 149 |
Find 29 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAAGGGAAAAGGACTTTC+TGG | 0.207237 | 7_3:+78403604 | Msa1126840:CDS |
| GCGCACACTCTTTGCTTTCT+CGG | 0.276894 | 7_3:-78403555 | None:intergenic |
| GACCGAGGCGACTACAATTC+CGG | 0.339363 | 7_3:+78403503 | Msa1126840:CDS |
| TGAAGACTCAAAATGAAAGT+TGG | 0.349810 | 7_3:-78403264 | None:intergenic |
| AAAATGAAAGTTGGGCTTCT+CGG | 0.382812 | 7_3:-78403255 | None:intergenic |
| TTGAAGTCTTTGCATATGTC+AGG | 0.387439 | 7_3:-78403434 | None:intergenic |
| GAAGACTCAAAATGAAAGTT+GGG | 0.391037 | 7_3:-78403263 | None:intergenic |
| TGACATATGCAAAGACTTCA+AGG | 0.411330 | 7_3:+78403436 | Msa1126840:CDS |
| ACCGAGGCGACTACAATTCC+GGG | 0.427387 | 7_3:+78403504 | Msa1126840:CDS |
| TAGCAAAGCAACAGCAACTT+TGG | 0.436558 | 7_3:+78403310 | Msa1126840:CDS |
| TGCAAAGACTTCAAGGAGAC+CGG | 0.477457 | 7_3:+78403443 | Msa1126840:CDS |
| GGTGGCAGTTGGACAAAGAA+TGG | 0.496757 | 7_3:+78403525 | Msa1126840:CDS |
| AAAGAGTGTGCGCGCGCTGA+TGG | 0.507062 | 7_3:+78403565 | Msa1126840:CDS |
| TTCAAGGAGACCGGGTATTG+TGG | 0.545327 | 7_3:+78403452 | Msa1126840:CDS |
| CTACAATTCCGGGTGGCAGT+TGG | 0.548426 | 7_3:+78403514 | Msa1126840:CDS |
| ACCCGGAATTGTAGTCGCCT+CGG | 0.550971 | 7_3:-78403505 | None:intergenic |
| CGCGCTGATGGTTTGTCTGA+AGG | 0.556877 | 7_3:+78403577 | Msa1126840:CDS |
| TTCTTTGTCCAACTGCCACC+CGG | 0.562902 | 7_3:-78403522 | None:intergenic |
| TGCAAGTTTCTTCACGACCG+AGG | 0.564969 | 7_3:+78403488 | Msa1126840:CDS |
| GAGACCGGGTATTGTGGCTA+CGG | 0.578795 | 7_3:+78403458 | Msa1126840:CDS |
| ATCGCCGTAGCCACAATACC+CGG | 0.586901 | 7_3:-78403462 | None:intergenic |
| GAAACTCTAATATGAGTCGA+AGG | 0.595574 | 7_3:-78403392 | None:intergenic |
| ACATGTAAATTACAAACACC+CGG | 0.625741 | 7_3:-78403645 | None:intergenic |
| GCAAAGACTTCAAGGAGACC+GGG | 0.633254 | 7_3:+78403444 | Msa1126840:CDS |
| ATCCGTGGGTCATACTCACA+CGG | 0.649413 | 7_3:+78403359 | Msa1126840:CDS |
| GTGTGAGTATGACCCACGGA+TGG | 0.652577 | 7_3:-78403357 | None:intergenic |
| GACCGTGTGAGTATGACCCA+CGG | 0.674066 | 7_3:-78403361 | None:intergenic |
| ATATGAGTCGAAGGTCTGAG+AGG | 0.697705 | 7_3:-78403383 | None:intergenic |
| GAGGCGACTACAATTCCGGG+TGG | 0.758896 | 7_3:+78403507 | Msa1126840:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGAGAAAAAGAACATGAAGA+AGG | + | chr7_3:78403214-78403233 | Msa1126840:CDS | 30.0% | |
| GAAGACTCAAAATGAAAGTT+GGG | - | chr7_3:78403266-78403285 | None:intergenic | 30.0% | |
| TGAAGACTCAAAATGAAAGT+TGG | - | chr7_3:78403267-78403286 | None:intergenic | 30.0% | |
| TTTGTCTGAAGGAAAAAAGA+AGG | + | chr7_3:78403588-78403607 | Msa1126840:CDS | 30.0% | |
| TTGTCTGAAGGAAAAAAGAA+GGG | + | chr7_3:78403589-78403608 | Msa1126840:CDS | 30.0% | |
| AAAATGAAAGTTGGGCTTCT+CGG | - | chr7_3:78403258-78403277 | None:intergenic | 35.0% | |
| !!! | ATTTTTCAAGTTCCATCCGT+GGG | + | chr7_3:78403345-78403364 | Msa1126840:CDS | 35.0% |
| GAAACTCTAATATGAGTCGA+AGG | - | chr7_3:78403395-78403414 | None:intergenic | 35.0% | |
| TTGAAGTCTTTGCATATGTC+AGG | - | chr7_3:78403437-78403456 | None:intergenic | 35.0% | |
| TGACATATGCAAAGACTTCA+AGG | + | chr7_3:78403436-78403455 | Msa1126840:CDS | 35.0% | |
| GAAGGAAAAAAGAAGGGAAA+AGG | + | chr7_3:78403595-78403614 | Msa1126840:CDS | 35.0% | |
| !! | GATTTTGCTGACTACAATTC+CGG | + | chr7_3:78403626-78403645 | Msa1126840:CDS | 35.0% |
| !! | ATTTTGCTGACTACAATTCC+GGG | + | chr7_3:78403627-78403646 | Msa1126840:CDS | 35.0% |
| TAGCAAAGCAACAGCAACTT+TGG | + | chr7_3:78403310-78403329 | Msa1126840:CDS | 40.0% | |
| !!! | CATTTTTCAAGTTCCATCCG+TGG | + | chr7_3:78403344-78403363 | Msa1126840:CDS | 40.0% |
| AAGAAGGGAAAAGGACTTTC+TGG | + | chr7_3:78403604-78403623 | Msa1126840:CDS | 40.0% | |
| ATATGAGTCGAAGGTCTGAG+AGG | - | chr7_3:78403386-78403405 | None:intergenic | 45.0% | |
| TGCAAAGACTTCAAGGAGAC+CGG | + | chr7_3:78403443-78403462 | Msa1126840:CDS | 45.0% | |
| ATCCGTGGGTCATACTCACA+CGG | + | chr7_3:78403359-78403378 | Msa1126840:CDS | 50.0% | |
| GCAAAGACTTCAAGGAGACC+GGG | + | chr7_3:78403444-78403463 | Msa1126840:CDS | 50.0% | |
| ! | TTCAAGGAGACCGGGTATTG+TGG | + | chr7_3:78403452-78403471 | Msa1126840:CDS | 50.0% |
| TGCAAGTTTCTTCACGACCG+AGG | + | chr7_3:78403488-78403507 | Msa1126840:CDS | 50.0% | |
| TTCTTTGTCCAACTGCCACC+CGG | - | chr7_3:78403525-78403544 | None:intergenic | 50.0% | |
| GGTGGCAGTTGGACAAAGAA+TGG | + | chr7_3:78403525-78403544 | Msa1126840:CDS | 50.0% | |
| GCGCACACTCTTTGCTTTCT+CGG | - | chr7_3:78403558-78403577 | None:intergenic | 50.0% | |
| GTGTGAGTATGACCCACGGA+TGG | - | chr7_3:78403360-78403379 | None:intergenic | 55.0% | |
| GACCGTGTGAGTATGACCCA+CGG | - | chr7_3:78403364-78403383 | None:intergenic | 55.0% | |
| ! | GAGACCGGGTATTGTGGCTA+CGG | + | chr7_3:78403458-78403477 | Msa1126840:CDS | 55.0% |
| ATCGCCGTAGCCACAATACC+CGG | - | chr7_3:78403465-78403484 | None:intergenic | 55.0% | |
| GACCGAGGCGACTACAATTC+CGG | + | chr7_3:78403503-78403522 | Msa1126840:CDS | 55.0% | |
| ACCGAGGCGACTACAATTCC+GGG | + | chr7_3:78403504-78403523 | Msa1126840:CDS | 55.0% | |
| ACCCGGAATTGTAGTCGCCT+CGG | - | chr7_3:78403508-78403527 | None:intergenic | 55.0% | |
| CTACAATTCCGGGTGGCAGT+TGG | + | chr7_3:78403514-78403533 | Msa1126840:CDS | 55.0% | |
| ! | CGCGCTGATGGTTTGTCTGA+AGG | + | chr7_3:78403577-78403596 | Msa1126840:CDS | 55.0% |
| GAGGCGACTACAATTCCGGG+TGG | + | chr7_3:78403507-78403526 | Msa1126840:CDS | 60.0% | |
| ! | AAAGAGTGTGCGCGCGCTGA+TGG | + | chr7_3:78403565-78403584 | Msa1126840:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_3 | gene | 78403206 | 78403658 | 78403206 | ID=Msa1126840;Name=Msa1126840 |
| chr7_3 | mRNA | 78403206 | 78403658 | 78403206 | ID=Msa1126840-mRNA-1;Parent=Msa1126840;Name=Msa1126840-mRNA-1;_AED=0.45;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|150 |
| chr7_3 | exon | 78403206 | 78403658 | 78403206 | ID=Msa1126840-mRNA-1:exon:21920;Parent=Msa1126840-mRNA-1 |
| chr7_3 | CDS | 78403206 | 78403658 | 78403206 | ID=Msa1126840-mRNA-1:cds;Parent=Msa1126840-mRNA-1 |
| Gene Sequence |
| Protein sequence |
