AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0582050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0582050 MtrunA17_Chr8g0377361 100.000 92 0 0 1 92 1 92 2.55e-67 196
Msa0582050 MtrunA17_Chr3g0095021 90.000 50 5 0 1 50 53 102 4.22e-29 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0582050 AT1G67350.2 73.810 84 22 0 1 84 1 84 1.38e-45 142
Msa0582050 AT1G67350.1 73.810 84 22 0 1 84 1 84 1.38e-45 142

Find 50 sgRNAs with CRISPR-Local

Find 273 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTTAAGATTTAACTGTTC+TGG 0.229897 4_1:+87712408 Msa0582050:three_prime_UTR
CATCGTCACAGAAGTGAAAA+TGG 0.245780 4_1:+87710774 Msa0582050:intron
TAGAACTATGGTGTTATATT+AGG 0.246291 4_1:+87712269 Msa0582050:three_prime_UTR
CTGCTGCACCGTTAAGAAAA+AGG 0.287608 4_1:+87710661 Msa0582050:five_prime_UTR
AGTTACCCTATGCGACCTTA+TGG 0.310546 4_1:+87710925 Msa0582050:CDS
GGGTCTGTAATGAACTATTT+TGG 0.311085 4_1:+87712227 Msa0582050:three_prime_UTR
AATCTCTTTATTTACATAGT+TGG 0.340518 4_1:+87712364 Msa0582050:three_prime_UTR
AATCTACGATCCCTTTCATT+TGG 0.347988 4_1:-87710847 None:intergenic
CTATGCGACCTTATGGGTTC+TGG 0.350279 4_1:+87710932 Msa0582050:CDS
AGTGAAAATGGCTTTCATAA+TGG 0.354869 4_1:+87710786 Msa0582050:exon
TAGTGGCCAATTGTGCTTCC+TGG 0.360865 4_1:+87712316 Msa0582050:three_prime_UTR
ATGAAAGGGATCGTAGATTC+AGG 0.366060 4_1:+87710851 Msa0582050:CDS
ATCTCTTTATTTACATAGTT+GGG 0.371188 4_1:+87712365 Msa0582050:three_prime_UTR
AATGGAATTCGCTGAGAATC+TGG 0.378487 4_1:+87710804 Msa0582050:CDS
ATATTAGGTGTTTCTCCATT+GGG 0.409394 4_1:+87712284 Msa0582050:three_prime_UTR
AAACAGCCAGGAAGCACAAT+TGG 0.424037 4_1:-87712322 None:intergenic
TAACGGTGCAGCAGTGTCTC+TGG 0.427524 4_1:-87710652 None:intergenic
GTTACCCTATGCGACCTTAT+GGG 0.431260 4_1:+87710926 Msa0582050:CDS
TATATTAGGTGTTTCTCCAT+TGG 0.438705 4_1:+87712283 Msa0582050:three_prime_UTR
CCTCATTTCGACGACGAGGC+AGG 0.443390 4_1:-87711051 None:intergenic
TGCAGCAGTGTCTCTGGAAT+CGG 0.454691 4_1:-87710646 None:intergenic
GAATCTGGTTCTGAAATTGA+TGG 0.457082 4_1:+87710819 Msa0582050:CDS
CCATTGGGAATATAAATTAG+TGG 0.458740 4_1:+87712299 Msa0582050:three_prime_UTR
CCCAAGCAAGCTGAGAGTTA+TGG 0.460911 4_1:-87710966 None:intergenic
GCCATAACTCTCAGCTTGCT+TGG 0.460971 4_1:+87710965 Msa0582050:CDS
CCATAACTCTCAGCTTGCTT+GGG 0.463504 4_1:+87710966 Msa0582050:CDS
AATGTAACATGCAACAGAAC+AGG 0.481138 4_1:-87712195 None:intergenic
GCTCAGATTAGTCAAGTTGC+CGG 0.484929 4_1:+87710991 Msa0582050:CDS
TGTGAAAGGATTTCATCATA+AGG 0.496680 4_1:-87711024 None:intergenic
CCTGCCTCGTCGTCGAAATG+AGG 0.497822 4_1:+87711051 Msa0582050:CDS
CTCATTTCGACGACGAGGCA+GGG 0.505092 4_1:-87711050 None:intergenic
CCACTAATTTATATTCCCAA+TGG 0.511619 4_1:-87712299 None:intergenic
TCAAATGTCCAGAACCCATA+AGG 0.513007 4_1:-87710940 None:intergenic
GATGGAAGATCCAAATGAAA+GGG 0.524216 4_1:+87710837 Msa0582050:CDS
CATGTTACATTGACTACACC+GGG 0.527959 4_1:+87712207 Msa0582050:three_prime_UTR
TGATGGAAGATCCAAATGAA+AGG 0.535018 4_1:+87710836 Msa0582050:CDS
GGATAGATGCGCGAAGACGA+AGG 0.541002 4_1:+87710894 Msa0582050:CDS
ACGAAATCTCATCGAGGAGA+AGG 0.541522 4_1:+87710617 Msa0582050:five_prime_UTR
CATAAAACAGACAAACAGCC+AGG 0.549333 4_1:-87712334 None:intergenic
ATCATAAGGATCTCTACGAC+CGG 0.561870 4_1:-87711010 None:intergenic
TGAAAGGGATCGTAGATTCA+GGG 0.568833 4_1:+87710852 Msa0582050:CDS
CAGAACCCATAAGGTCGCAT+AGG 0.571457 4_1:-87710931 None:intergenic
GCGCGAAGACGAAGGAGATG+TGG 0.571625 4_1:+87710902 Msa0582050:CDS
GCATGTTACATTGACTACAC+CGG 0.580850 4_1:+87712206 Msa0582050:three_prime_UTR
AGAACCCATAAGGTCGCATA+GGG 0.582926 4_1:-87710930 None:intergenic
ACGAGGCAGGGTAATGTGAA+AGG 0.598363 4_1:-87711038 None:intergenic
AAAATAGTTCATTACAGACC+CGG 0.668711 4_1:-87712225 None:intergenic
GGAACATGTGTATAAGACGA+AGG 0.686694 4_1:+87710873 Msa0582050:CDS
AAAACCTCATTTCGACGACG+AGG 0.717022 4_1:-87711055 None:intergenic
GGTTGAACGAAATCTCATCG+AGG 0.795079 4_1:+87710611 Msa0582050:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAAACGTTTTTTTTTTTAT+AGG + chr4_1:87711386-87711405 Msa0582050:intron 10.0%
!!! ATAAACGTTTTTTTTTTTAT+AGG + chr4_1:87711386-87711405 Msa0582050:intron 10.0%
!!! TCAATTTAATTTCACAATTT+AGG + chr4_1:87710720-87710739 Msa0582050:intron 15.0%
!!! CAATTTAATTTCACAATTTA+GGG + chr4_1:87710721-87710740 Msa0582050:intron 15.0%
!! CAAATAAAAATAACATGTTA+GGG - chr4_1:87711093-87711112 None:intergenic 15.0%
!! TCAAATAAAAATAACATGTT+AGG - chr4_1:87711094-87711113 None:intergenic 15.0%
!!! AACATGTTATTTTTATTTGA+TGG + chr4_1:87711094-87711113 Msa0582050:intron 15.0%
!!! AATTAGTAATAATTTGTTAG+AGG + chr4_1:87711420-87711439 Msa0582050:intron 15.0%
!!! ATTAGTAATAATTTGTTAGA+GGG + chr4_1:87711421-87711440 Msa0582050:intron 15.0%
!! TATAAATTAAGGAAAAAGAT+GGG - chr4_1:87711957-87711976 None:intergenic 15.0%
!! TTATAAATTAAGGAAAAAGA+TGG - chr4_1:87711958-87711977 None:intergenic 15.0%
!!! AATAAATTTTCATAGAACTA+TGG + chr4_1:87712257-87712276 Msa0582050:three_prime_UTR 15.0%
!!! ATTTAATTCAATTTTTGTCT+TGG + chr4_1:87712440-87712459 Msa0582050:three_prime_UTR 15.0%
!!! TCAATTTAATTTCACAATTT+AGG + chr4_1:87710720-87710739 Msa0582050:intron 15.0%
!!! CAATTTAATTTCACAATTTA+GGG + chr4_1:87710721-87710740 Msa0582050:intron 15.0%
!! CAAATAAAAATAACATGTTA+GGG - chr4_1:87711093-87711112 None:intergenic 15.0%
!! TCAAATAAAAATAACATGTT+AGG - chr4_1:87711094-87711113 None:intergenic 15.0%
!!! AACATGTTATTTTTATTTGA+TGG + chr4_1:87711094-87711113 Msa0582050:intron 15.0%
!!! AATTAGTAATAATTTGTTAG+AGG + chr4_1:87711420-87711439 Msa0582050:intron 15.0%
!!! ATTAGTAATAATTTGTTAGA+GGG + chr4_1:87711421-87711440 Msa0582050:intron 15.0%
!! TATAAATTAAGGAAAAAGAT+GGG - chr4_1:87711957-87711976 None:intergenic 15.0%
!! TTATAAATTAAGGAAAAAGA+TGG - chr4_1:87711958-87711977 None:intergenic 15.0%
!!! AATAAATTTTCATAGAACTA+TGG + chr4_1:87712257-87712276 Msa0582050:three_prime_UTR 15.0%
!!! TGTTTTTACCTTTTTCTTAA+CGG - chr4_1:87710672-87710691 None:intergenic 20.0%
!! TTTATATAACCGATCTAATT+TGG + chr4_1:87710744-87710763 Msa0582050:intron 20.0%
!! TTATATAACCGATCTAATTT+GGG + chr4_1:87710745-87710764 Msa0582050:intron 20.0%
!! AAAGATTAAGTTTCTCATAT+AGG - chr4_1:87711543-87711562 None:intergenic 20.0%
!! TGGAAGAGATTTATAAATTA+AGG - chr4_1:87711968-87711987 None:intergenic 20.0%
!!! AATCTCTTTATTTACATAGT+TGG + chr4_1:87712364-87712383 Msa0582050:three_prime_UTR 20.0%
!!! ATCTCTTTATTTACATAGTT+GGG + chr4_1:87712365-87712384 Msa0582050:three_prime_UTR 20.0%
!!! TGTTTTTACCTTTTTCTTAA+CGG - chr4_1:87710672-87710691 None:intergenic 20.0%
!! TTTATATAACCGATCTAATT+TGG + chr4_1:87710744-87710763 Msa0582050:intron 20.0%
!! TTATATAACCGATCTAATTT+GGG + chr4_1:87710745-87710764 Msa0582050:intron 20.0%
!! AAAGATTAAGTTTCTCATAT+AGG - chr4_1:87711543-87711562 None:intergenic 20.0%
!! TGGAAGAGATTTATAAATTA+AGG - chr4_1:87711968-87711987 None:intergenic 20.0%
!!! AATCTCTTTATTTACATAGT+TGG + chr4_1:87712364-87712383 Msa0582050:three_prime_UTR 20.0%
!!! ATCTCTTTATTTACATAGTT+GGG + chr4_1:87712365-87712384 Msa0582050:three_prime_UTR 20.0%
! TATATAACCGATCTAATTTG+GGG + chr4_1:87710746-87710765 Msa0582050:intron 25.0%
!! TTTATTTGATGGCTATATGT+TGG + chr4_1:87711105-87711124 Msa0582050:intron 25.0%
! GTTTATCATAGAGTAAATGA+CGG - chr4_1:87711372-87711391 None:intergenic 25.0%
!! GAGAAACTTAATCTTTAAAG+TGG + chr4_1:87711547-87711566 Msa0582050:intron 25.0%
! ATAACCGTATTTAACAACAA+TGG + chr4_1:87711754-87711773 Msa0582050:intron 25.0%
! CATCAGTTAATAGGAAAAAA+AGG - chr4_1:87711822-87711841 None:intergenic 25.0%
! CATGTTTATCATCAGTTAAT+AGG - chr4_1:87711831-87711850 None:intergenic 25.0%
! TAACTGATGATAAACATGTA+TGG + chr4_1:87711833-87711852 Msa0582050:intron 25.0%
!!! GTTTTCAAGCAATACAATAA+AGG - chr4_1:87711884-87711903 None:intergenic 25.0%
! AACTTTCAACAATCTATGAT+TGG - chr4_1:87711988-87712007 None:intergenic 25.0%
! TGAAAAACTGGAACTATATT+GGG + chr4_1:87712136-87712155 Msa0582050:three_prime_UTR 25.0%
!!! TAGAACTATGGTGTTATATT+AGG + chr4_1:87712269-87712288 Msa0582050:three_prime_UTR 25.0%
!! TGTTTAAGATTTAACTGTTC+TGG + chr4_1:87712408-87712427 Msa0582050:three_prime_UTR 25.0%
! TATATAACCGATCTAATTTG+GGG + chr4_1:87710746-87710765 Msa0582050:intron 25.0%
!! TTTATTTGATGGCTATATGT+TGG + chr4_1:87711105-87711124 Msa0582050:intron 25.0%
! GTTTATCATAGAGTAAATGA+CGG - chr4_1:87711372-87711391 None:intergenic 25.0%
!! GAGAAACTTAATCTTTAAAG+TGG + chr4_1:87711547-87711566 Msa0582050:intron 25.0%
! ATAACCGTATTTAACAACAA+TGG + chr4_1:87711754-87711773 Msa0582050:intron 25.0%
! CATCAGTTAATAGGAAAAAA+AGG - chr4_1:87711822-87711841 None:intergenic 25.0%
! CATGTTTATCATCAGTTAAT+AGG - chr4_1:87711831-87711850 None:intergenic 25.0%
! TAACTGATGATAAACATGTA+TGG + chr4_1:87711833-87711852 Msa0582050:intron 25.0%
!!! GTTTTCAAGCAATACAATAA+AGG - chr4_1:87711884-87711903 None:intergenic 25.0%
! AACTTTCAACAATCTATGAT+TGG - chr4_1:87711988-87712007 None:intergenic 25.0%
! TGAAAAACTGGAACTATATT+GGG + chr4_1:87712136-87712155 Msa0582050:three_prime_UTR 25.0%
!!! TAGAACTATGGTGTTATATT+AGG + chr4_1:87712269-87712288 Msa0582050:three_prime_UTR 25.0%
!! TGTTTAAGATTTAACTGTTC+TGG + chr4_1:87712408-87712427 Msa0582050:three_prime_UTR 25.0%
ATGAAATTGAGAAATGAGAG+TGG - chr4_1:87710698-87710717 None:intergenic 30.0%
TGAAAATCCCCAAATTAGAT+CGG - chr4_1:87710756-87710775 None:intergenic 30.0%
AGTGAAAATGGCTTTCATAA+TGG + chr4_1:87710786-87710805 Msa0582050:exon 30.0%
TGTGAAAGGATTTCATCATA+AGG - chr4_1:87711027-87711046 None:intergenic 30.0%
!! AGTAATAATTTGTTAGAGGG+AGG + chr4_1:87711424-87711443 Msa0582050:intron 30.0%
!! GTAATAATTTGTTAGAGGGA+GGG + chr4_1:87711425-87711444 Msa0582050:intron 30.0%
!! TAATAATTTGTTAGAGGGAG+GGG + chr4_1:87711426-87711445 Msa0582050:intron 30.0%
ATTGTTCTGCATTAGCTATT+TGG - chr4_1:87711688-87711707 None:intergenic 30.0%
GTTGTTAAATACGGTTATAG+TGG - chr4_1:87711752-87711771 None:intergenic 30.0%
! TCAGCCATTGTTGTTAAATA+CGG - chr4_1:87711761-87711780 None:intergenic 30.0%
AATGCATTGCATGATATGAT+AGG - chr4_1:87711915-87711934 None:intergenic 30.0%
AAAAAGATGGGAAAAAATGG+TGG - chr4_1:87711945-87711964 None:intergenic 30.0%
AGGAAAAAGATGGGAAAAAA+TGG - chr4_1:87711948-87711967 None:intergenic 30.0%
AAGTGTTCTTTGTGAAAAAC+TGG + chr4_1:87712124-87712143 Msa0582050:three_prime_UTR 30.0%
GTGAAAAACTGGAACTATAT+TGG + chr4_1:87712135-87712154 Msa0582050:three_prime_UTR 30.0%
AAAATAGTTCATTACAGACC+CGG - chr4_1:87712228-87712247 None:intergenic 30.0%
TATATTAGGTGTTTCTCCAT+TGG + chr4_1:87712283-87712302 Msa0582050:three_prime_UTR 30.0%
ATATTAGGTGTTTCTCCATT+GGG + chr4_1:87712284-87712303 Msa0582050:three_prime_UTR 30.0%
CCACTAATTTATATTCCCAA+TGG - chr4_1:87712302-87712321 None:intergenic 30.0%
CCATTGGGAATATAAATTAG+TGG + chr4_1:87712299-87712318 Msa0582050:three_prime_UTR 30.0%
ATGAAATTGAGAAATGAGAG+TGG - chr4_1:87710698-87710717 None:intergenic 30.0%
TGAAAATCCCCAAATTAGAT+CGG - chr4_1:87710756-87710775 None:intergenic 30.0%
AGTGAAAATGGCTTTCATAA+TGG + chr4_1:87710786-87710805 Msa0582050:exon 30.0%
TGTGAAAGGATTTCATCATA+AGG - chr4_1:87711027-87711046 None:intergenic 30.0%
!! AGTAATAATTTGTTAGAGGG+AGG + chr4_1:87711424-87711443 Msa0582050:intron 30.0%
!! GTAATAATTTGTTAGAGGGA+GGG + chr4_1:87711425-87711444 Msa0582050:intron 30.0%
!! TAATAATTTGTTAGAGGGAG+GGG + chr4_1:87711426-87711445 Msa0582050:intron 30.0%
ATTGTTCTGCATTAGCTATT+TGG - chr4_1:87711688-87711707 None:intergenic 30.0%
GTTGTTAAATACGGTTATAG+TGG - chr4_1:87711752-87711771 None:intergenic 30.0%
! TCAGCCATTGTTGTTAAATA+CGG - chr4_1:87711761-87711780 None:intergenic 30.0%
AATGCATTGCATGATATGAT+AGG - chr4_1:87711915-87711934 None:intergenic 30.0%
AAAAAGATGGGAAAAAATGG+TGG - chr4_1:87711945-87711964 None:intergenic 30.0%
AGGAAAAAGATGGGAAAAAA+TGG - chr4_1:87711948-87711967 None:intergenic 30.0%
AAGTGTTCTTTGTGAAAAAC+TGG + chr4_1:87712124-87712143 Msa0582050:three_prime_UTR 30.0%
GTGAAAAACTGGAACTATAT+TGG + chr4_1:87712135-87712154 Msa0582050:three_prime_UTR 30.0%
AAAATAGTTCATTACAGACC+CGG - chr4_1:87712228-87712247 None:intergenic 30.0%
TATATTAGGTGTTTCTCCAT+TGG + chr4_1:87712283-87712302 Msa0582050:three_prime_UTR 30.0%
ATATTAGGTGTTTCTCCATT+GGG + chr4_1:87712284-87712303 Msa0582050:three_prime_UTR 30.0%
CCACTAATTTATATTCCCAA+TGG - chr4_1:87712302-87712321 None:intergenic 30.0%
CCATTGGGAATATAAATTAG+TGG + chr4_1:87712299-87712318 Msa0582050:three_prime_UTR 30.0%
!! GAATCTGGTTCTGAAATTGA+TGG + chr4_1:87710819-87710838 Msa0582050:CDS 35.0%
TGATGGAAGATCCAAATGAA+AGG + chr4_1:87710836-87710855 Msa0582050:CDS 35.0%
GATGGAAGATCCAAATGAAA+GGG + chr4_1:87710837-87710856 Msa0582050:CDS 35.0%
AATCTACGATCCCTTTCATT+TGG - chr4_1:87710850-87710869 None:intergenic 35.0%
TTGTGTGTAACTAGATGTGT+TGG + chr4_1:87711212-87711231 Msa0582050:intron 35.0%
!! AATAATTTGTTAGAGGGAGG+GGG + chr4_1:87711427-87711446 Msa0582050:intron 35.0%
GAATTCAGTGTTGTCAATTG+CGG + chr4_1:87711578-87711597 Msa0582050:intron 35.0%
!! TTCTGAAATAGCAGCTATAG+TGG - chr4_1:87711656-87711675 None:intergenic 35.0%
ACAGTGCATTAGCAAAACAA+AGG - chr4_1:87712039-87712058 None:intergenic 35.0%
AATGTAACATGCAACAGAAC+AGG - chr4_1:87712198-87712217 None:intergenic 35.0%
! GGGTCTGTAATGAACTATTT+TGG + chr4_1:87712227-87712246 Msa0582050:three_prime_UTR 35.0%
! TGGCTGTTTGTCTGTTTTAT+GGG + chr4_1:87712336-87712355 Msa0582050:three_prime_UTR 35.0%
!! GAATCTGGTTCTGAAATTGA+TGG + chr4_1:87710819-87710838 Msa0582050:CDS 35.0%
TGATGGAAGATCCAAATGAA+AGG + chr4_1:87710836-87710855 Msa0582050:CDS 35.0%
GATGGAAGATCCAAATGAAA+GGG + chr4_1:87710837-87710856 Msa0582050:CDS 35.0%
AATCTACGATCCCTTTCATT+TGG - chr4_1:87710850-87710869 None:intergenic 35.0%
TTGTGTGTAACTAGATGTGT+TGG + chr4_1:87711212-87711231 Msa0582050:intron 35.0%
!! AATAATTTGTTAGAGGGAGG+GGG + chr4_1:87711427-87711446 Msa0582050:intron 35.0%
GAATTCAGTGTTGTCAATTG+CGG + chr4_1:87711578-87711597 Msa0582050:intron 35.0%
!! TTCTGAAATAGCAGCTATAG+TGG - chr4_1:87711656-87711675 None:intergenic 35.0%
ACAGTGCATTAGCAAAACAA+AGG - chr4_1:87712039-87712058 None:intergenic 35.0%
AATGTAACATGCAACAGAAC+AGG - chr4_1:87712198-87712217 None:intergenic 35.0%
! GGGTCTGTAATGAACTATTT+TGG + chr4_1:87712227-87712246 Msa0582050:three_prime_UTR 35.0%
! TGGCTGTTTGTCTGTTTTAT+GGG + chr4_1:87712336-87712355 Msa0582050:three_prime_UTR 35.0%
CATCGTCACAGAAGTGAAAA+TGG + chr4_1:87710774-87710793 Msa0582050:intron 40.0%
AATGGAATTCGCTGAGAATC+TGG + chr4_1:87710804-87710823 Msa0582050:CDS 40.0%
ATGAAAGGGATCGTAGATTC+AGG + chr4_1:87710851-87710870 Msa0582050:CDS 40.0%
TGAAAGGGATCGTAGATTCA+GGG + chr4_1:87710852-87710871 Msa0582050:CDS 40.0%
GGAACATGTGTATAAGACGA+AGG + chr4_1:87710873-87710892 Msa0582050:CDS 40.0%
TCAAATGTCCAGAACCCATA+AGG - chr4_1:87710943-87710962 None:intergenic 40.0%
ATCATAAGGATCTCTACGAC+CGG - chr4_1:87711013-87711032 None:intergenic 40.0%
! GTAACTAGATGTGTTGGTGT+CGG + chr4_1:87711218-87711237 Msa0582050:intron 40.0%
! ATTGCTAGAAGGGTTTGGTT+GGG + chr4_1:87711279-87711298 Msa0582050:intron 40.0%
TTGGCTAAGATTAGAGTCGA+TGG + chr4_1:87711316-87711335 Msa0582050:intron 40.0%
AGTTGTATGCAAGAAGTCCA+AGG - chr4_1:87711476-87711495 None:intergenic 40.0%
CTTCTTGCATACAACTCCTT+TGG + chr4_1:87711480-87711499 Msa0582050:intron 40.0%
! TGTTGTCAATTGCGGATTGT+GGG + chr4_1:87711586-87711605 Msa0582050:intron 40.0%
GTTGCATTGCGGATTTGAAA+AGG - chr4_1:87711621-87711640 None:intergenic 40.0%
GGCGCTATAACACTATTGAA+TGG - chr4_1:87711731-87711750 None:intergenic 40.0%
AACAACAATGGCTGAGTTAC+TGG + chr4_1:87711766-87711785 Msa0582050:intron 40.0%
! GCATGTTACATTGACTACAC+CGG + chr4_1:87712206-87712225 Msa0582050:three_prime_UTR 40.0%
! CATGTTACATTGACTACACC+GGG + chr4_1:87712207-87712226 Msa0582050:three_prime_UTR 40.0%
CATAAAACAGACAAACAGCC+AGG - chr4_1:87712337-87712356 None:intergenic 40.0%
! CTGGCTGTTTGTCTGTTTTA+TGG + chr4_1:87712335-87712354 Msa0582050:three_prime_UTR 40.0%
CATCGTCACAGAAGTGAAAA+TGG + chr4_1:87710774-87710793 Msa0582050:intron 40.0%
AATGGAATTCGCTGAGAATC+TGG + chr4_1:87710804-87710823 Msa0582050:CDS 40.0%
ATGAAAGGGATCGTAGATTC+AGG + chr4_1:87710851-87710870 Msa0582050:CDS 40.0%
TGAAAGGGATCGTAGATTCA+GGG + chr4_1:87710852-87710871 Msa0582050:CDS 40.0%
GGAACATGTGTATAAGACGA+AGG + chr4_1:87710873-87710892 Msa0582050:CDS 40.0%
TCAAATGTCCAGAACCCATA+AGG - chr4_1:87710943-87710962 None:intergenic 40.0%
ATCATAAGGATCTCTACGAC+CGG - chr4_1:87711013-87711032 None:intergenic 40.0%
! GTAACTAGATGTGTTGGTGT+CGG + chr4_1:87711218-87711237 Msa0582050:intron 40.0%
! ATTGCTAGAAGGGTTTGGTT+GGG + chr4_1:87711279-87711298 Msa0582050:intron 40.0%
TTGGCTAAGATTAGAGTCGA+TGG + chr4_1:87711316-87711335 Msa0582050:intron 40.0%
AGTTGTATGCAAGAAGTCCA+AGG - chr4_1:87711476-87711495 None:intergenic 40.0%
CTTCTTGCATACAACTCCTT+TGG + chr4_1:87711480-87711499 Msa0582050:intron 40.0%
! TGTTGTCAATTGCGGATTGT+GGG + chr4_1:87711586-87711605 Msa0582050:intron 40.0%
GTTGCATTGCGGATTTGAAA+AGG - chr4_1:87711621-87711640 None:intergenic 40.0%
GGCGCTATAACACTATTGAA+TGG - chr4_1:87711731-87711750 None:intergenic 40.0%
AACAACAATGGCTGAGTTAC+TGG + chr4_1:87711766-87711785 Msa0582050:intron 40.0%
! GCATGTTACATTGACTACAC+CGG + chr4_1:87712206-87712225 Msa0582050:three_prime_UTR 40.0%
! CATGTTACATTGACTACACC+GGG + chr4_1:87712207-87712226 Msa0582050:three_prime_UTR 40.0%
CATAAAACAGACAAACAGCC+AGG - chr4_1:87712337-87712356 None:intergenic 40.0%
! CTGGCTGTTTGTCTGTTTTA+TGG + chr4_1:87712335-87712354 Msa0582050:three_prime_UTR 40.0%
GGTTGAACGAAATCTCATCG+AGG + chr4_1:87710611-87710630 Msa0582050:five_prime_UTR 45.0%
ACGAAATCTCATCGAGGAGA+AGG + chr4_1:87710617-87710636 Msa0582050:five_prime_UTR 45.0%
CTGCTGCACCGTTAAGAAAA+AGG + chr4_1:87710661-87710680 Msa0582050:five_prime_UTR 45.0%
AGTTACCCTATGCGACCTTA+TGG + chr4_1:87710925-87710944 Msa0582050:CDS 45.0%
GTTACCCTATGCGACCTTAT+GGG + chr4_1:87710926-87710945 Msa0582050:CDS 45.0%
AGAACCCATAAGGTCGCATA+GGG - chr4_1:87710933-87710952 None:intergenic 45.0%
! CCATAACTCTCAGCTTGCTT+GGG + chr4_1:87710966-87710985 Msa0582050:CDS 45.0%
! GCTCAGATTAGTCAAGTTGC+CGG + chr4_1:87710991-87711010 Msa0582050:CDS 45.0%
AAAACCTCATTTCGACGACG+AGG - chr4_1:87711058-87711077 None:intergenic 45.0%
!! GTTGGTAAGAAGCACTGACA+TGG + chr4_1:87711123-87711142 Msa0582050:intron 45.0%
GCACGTTCGATATGAAGTTC+CGG + chr4_1:87711250-87711269 Msa0582050:intron 45.0%
GCTACATTGCTAGAAGGGTT+TGG + chr4_1:87711274-87711293 Msa0582050:intron 45.0%
! CATTGCTAGAAGGGTTTGGT+TGG + chr4_1:87711278-87711297 Msa0582050:intron 45.0%
! TAGAAGGGTTTGGTTGGGAT+GGG + chr4_1:87711284-87711303 Msa0582050:intron 45.0%
!! AGGGGGAAATGTTGGTAGTA+GGG + chr4_1:87711444-87711463 Msa0582050:intron 45.0%
TTTCTCATATAGGTCCCCGA+AGG - chr4_1:87711533-87711552 None:intergenic 45.0%
! GTGTTGTCAATTGCGGATTG+TGG + chr4_1:87711585-87711604 Msa0582050:intron 45.0%
! TTGCGGATTGTGGGTAATAG+TGG + chr4_1:87711595-87711614 Msa0582050:intron 45.0%
! AAACAGCCAGGAAGCACAAT+TGG - chr4_1:87712325-87712344 None:intergenic 45.0%
GGTTGAACGAAATCTCATCG+AGG + chr4_1:87710611-87710630 Msa0582050:five_prime_UTR 45.0%
ACGAAATCTCATCGAGGAGA+AGG + chr4_1:87710617-87710636 Msa0582050:five_prime_UTR 45.0%
CTGCTGCACCGTTAAGAAAA+AGG + chr4_1:87710661-87710680 Msa0582050:five_prime_UTR 45.0%
AGTTACCCTATGCGACCTTA+TGG + chr4_1:87710925-87710944 Msa0582050:CDS 45.0%
GTTACCCTATGCGACCTTAT+GGG + chr4_1:87710926-87710945 Msa0582050:CDS 45.0%
AGAACCCATAAGGTCGCATA+GGG - chr4_1:87710933-87710952 None:intergenic 45.0%
! CCATAACTCTCAGCTTGCTT+GGG + chr4_1:87710966-87710985 Msa0582050:CDS 45.0%
! GCTCAGATTAGTCAAGTTGC+CGG + chr4_1:87710991-87711010 Msa0582050:CDS 45.0%
AAAACCTCATTTCGACGACG+AGG - chr4_1:87711058-87711077 None:intergenic 45.0%
!! GTTGGTAAGAAGCACTGACA+TGG + chr4_1:87711123-87711142 Msa0582050:intron 45.0%
GCACGTTCGATATGAAGTTC+CGG + chr4_1:87711250-87711269 Msa0582050:intron 45.0%
GCTACATTGCTAGAAGGGTT+TGG + chr4_1:87711274-87711293 Msa0582050:intron 45.0%
! CATTGCTAGAAGGGTTTGGT+TGG + chr4_1:87711278-87711297 Msa0582050:intron 45.0%
! TAGAAGGGTTTGGTTGGGAT+GGG + chr4_1:87711284-87711303 Msa0582050:intron 45.0%
!! AGGGGGAAATGTTGGTAGTA+GGG + chr4_1:87711444-87711463 Msa0582050:intron 45.0%
TTTCTCATATAGGTCCCCGA+AGG - chr4_1:87711533-87711552 None:intergenic 45.0%
! GTGTTGTCAATTGCGGATTG+TGG + chr4_1:87711585-87711604 Msa0582050:intron 45.0%
! TTGCGGATTGTGGGTAATAG+TGG + chr4_1:87711595-87711614 Msa0582050:intron 45.0%
! AAACAGCCAGGAAGCACAAT+TGG - chr4_1:87712325-87712344 None:intergenic 45.0%
!! TGCAGCAGTGTCTCTGGAAT+CGG - chr4_1:87710649-87710668 None:intergenic 50.0%
CAGAACCCATAAGGTCGCAT+AGG - chr4_1:87710934-87710953 None:intergenic 50.0%
CTATGCGACCTTATGGGTTC+TGG + chr4_1:87710932-87710951 Msa0582050:CDS 50.0%
! GCCATAACTCTCAGCTTGCT+TGG + chr4_1:87710965-87710984 Msa0582050:CDS 50.0%
CCCAAGCAAGCTGAGAGTTA+TGG - chr4_1:87710969-87710988 None:intergenic 50.0%
ACGAGGCAGGGTAATGTGAA+AGG - chr4_1:87711041-87711060 None:intergenic 50.0%
!! GATGTGTTGGTGTCGGACAT+TGG + chr4_1:87711225-87711244 Msa0582050:intron 50.0%
!! TCCGGTGCTACATTGCTAGA+AGG + chr4_1:87711268-87711287 Msa0582050:intron 50.0%
CCCTTCTAGCAATGTAGCAC+CGG - chr4_1:87711272-87711291 None:intergenic 50.0%
!! CCGGTGCTACATTGCTAGAA+GGG + chr4_1:87711269-87711288 Msa0582050:intron 50.0%
! CTAGAAGGGTTTGGTTGGGA+TGG + chr4_1:87711283-87711302 Msa0582050:intron 50.0%
GTTTGGTTGGGATGGGATTG+TGG + chr4_1:87711291-87711310 Msa0582050:intron 50.0%
TTGGGATGGGATTGTGGACT+TGG + chr4_1:87711297-87711316 Msa0582050:intron 50.0%
TTAGAGGGAGGGGGAAATGT+TGG + chr4_1:87711436-87711455 Msa0582050:intron 50.0%
!! GAGGGGGAAATGTTGGTAGT+AGG + chr4_1:87711443-87711462 Msa0582050:intron 50.0%
!! GGGGGAAATGTTGGTAGTAG+GGG + chr4_1:87711445-87711464 Msa0582050:intron 50.0%
TGGTAGTAGGGGTTGAACCT+TGG + chr4_1:87711456-87711475 Msa0582050:intron 50.0%
AAACCACCGAGCTACTCCTT+CGG + chr4_1:87711514-87711533 Msa0582050:intron 50.0%
GCTACAGCACTGTTGCATTG+CGG - chr4_1:87711632-87711651 None:intergenic 50.0%
TAGTGGCCAATTGTGCTTCC+TGG + chr4_1:87712316-87712335 Msa0582050:three_prime_UTR 50.0%
!! TGCAGCAGTGTCTCTGGAAT+CGG - chr4_1:87710649-87710668 None:intergenic 50.0%
CAGAACCCATAAGGTCGCAT+AGG - chr4_1:87710934-87710953 None:intergenic 50.0%
CTATGCGACCTTATGGGTTC+TGG + chr4_1:87710932-87710951 Msa0582050:CDS 50.0%
! GCCATAACTCTCAGCTTGCT+TGG + chr4_1:87710965-87710984 Msa0582050:CDS 50.0%
CCCAAGCAAGCTGAGAGTTA+TGG - chr4_1:87710969-87710988 None:intergenic 50.0%
ACGAGGCAGGGTAATGTGAA+AGG - chr4_1:87711041-87711060 None:intergenic 50.0%
!! GATGTGTTGGTGTCGGACAT+TGG + chr4_1:87711225-87711244 Msa0582050:intron 50.0%
!! TCCGGTGCTACATTGCTAGA+AGG + chr4_1:87711268-87711287 Msa0582050:intron 50.0%
CCCTTCTAGCAATGTAGCAC+CGG - chr4_1:87711272-87711291 None:intergenic 50.0%
!! CCGGTGCTACATTGCTAGAA+GGG + chr4_1:87711269-87711288 Msa0582050:intron 50.0%
! CTAGAAGGGTTTGGTTGGGA+TGG + chr4_1:87711283-87711302 Msa0582050:intron 50.0%
GTTTGGTTGGGATGGGATTG+TGG + chr4_1:87711291-87711310 Msa0582050:intron 50.0%
TTGGGATGGGATTGTGGACT+TGG + chr4_1:87711297-87711316 Msa0582050:intron 50.0%
TTAGAGGGAGGGGGAAATGT+TGG + chr4_1:87711436-87711455 Msa0582050:intron 50.0%
!! GAGGGGGAAATGTTGGTAGT+AGG + chr4_1:87711443-87711462 Msa0582050:intron 50.0%
!! GGGGGAAATGTTGGTAGTAG+GGG + chr4_1:87711445-87711464 Msa0582050:intron 50.0%
TGGTAGTAGGGGTTGAACCT+TGG + chr4_1:87711456-87711475 Msa0582050:intron 50.0%
AAACCACCGAGCTACTCCTT+CGG + chr4_1:87711514-87711533 Msa0582050:intron 50.0%
GCTACAGCACTGTTGCATTG+CGG - chr4_1:87711632-87711651 None:intergenic 50.0%
TAGTGGCCAATTGTGCTTCC+TGG + chr4_1:87712316-87712335 Msa0582050:three_prime_UTR 50.0%
TAACGGTGCAGCAGTGTCTC+TGG - chr4_1:87710655-87710674 None:intergenic 55.0%
GGATAGATGCGCGAAGACGA+AGG + chr4_1:87710894-87710913 Msa0582050:CDS 55.0%
CTCATTTCGACGACGAGGCA+GGG - chr4_1:87711053-87711072 None:intergenic 55.0%
!! GGTTTGGCTTGGGACACCAA+AGG - chr4_1:87711499-87711518 None:intergenic 55.0%
! GTAGCTCGGTGGTTTGGCTT+GGG - chr4_1:87711509-87711528 None:intergenic 55.0%
! AGTAGCTCGGTGGTTTGGCT+TGG - chr4_1:87711510-87711529 None:intergenic 55.0%
! GAAGGAGTAGCTCGGTGGTT+TGG - chr4_1:87711515-87711534 None:intergenic 55.0%
AACCACCGAGCTACTCCTTC+GGG + chr4_1:87711515-87711534 Msa0582050:intron 55.0%
TAACGGTGCAGCAGTGTCTC+TGG - chr4_1:87710655-87710674 None:intergenic 55.0%
GGATAGATGCGCGAAGACGA+AGG + chr4_1:87710894-87710913 Msa0582050:CDS 55.0%
CTCATTTCGACGACGAGGCA+GGG - chr4_1:87711053-87711072 None:intergenic 55.0%
!! GGTTTGGCTTGGGACACCAA+AGG - chr4_1:87711499-87711518 None:intergenic 55.0%
! GTAGCTCGGTGGTTTGGCTT+GGG - chr4_1:87711509-87711528 None:intergenic 55.0%
! AGTAGCTCGGTGGTTTGGCT+TGG - chr4_1:87711510-87711529 None:intergenic 55.0%
! GAAGGAGTAGCTCGGTGGTT+TGG - chr4_1:87711515-87711534 None:intergenic 55.0%
AACCACCGAGCTACTCCTTC+GGG + chr4_1:87711515-87711534 Msa0582050:intron 55.0%
GCGCGAAGACGAAGGAGATG+TGG + chr4_1:87710902-87710921 Msa0582050:CDS 60.0%
CCTCATTTCGACGACGAGGC+AGG - chr4_1:87711054-87711073 None:intergenic 60.0%
CCTGCCTCGTCGTCGAAATG+AGG + chr4_1:87711051-87711070 Msa0582050:CDS 60.0%
ACCACCGAGCTACTCCTTCG+GGG + chr4_1:87711516-87711535 Msa0582050:intron 60.0%
AGGTCCCCGAAGGAGTAGCT+CGG - chr4_1:87711523-87711542 None:intergenic 60.0%
GCGCGAAGACGAAGGAGATG+TGG + chr4_1:87710902-87710921 Msa0582050:CDS 60.0%
CCTCATTTCGACGACGAGGC+AGG - chr4_1:87711054-87711073 None:intergenic 60.0%
CCTGCCTCGTCGTCGAAATG+AGG + chr4_1:87711051-87711070 Msa0582050:CDS 60.0%
ACCACCGAGCTACTCCTTCG+GGG + chr4_1:87711516-87711535 Msa0582050:intron 60.0%
AGGTCCCCGAAGGAGTAGCT+CGG - chr4_1:87711523-87711542 None:intergenic 60.0%
TCCCCGAAGGAGTAGCTCGG+TGG - chr4_1:87711520-87711539 None:intergenic 65.0%
TCCCCGAAGGAGTAGCTCGG+TGG - chr4_1:87711520-87711539 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 87710595 87712462 87710595 ID=Msa0582050;Name=Msa0582050
chr4_1 mRNA 87710595 87712462 87710595 ID=Msa0582050-mRNA-1;Parent=Msa0582050;Name=Msa0582050-mRNA-1;_AED=0.00;_eAED=0.00;_QI=96|0.5|0.66|1|0|0.33|3|364|92
chr4_1 exon 87710595 87710682 87710595 ID=Msa0582050-mRNA-1:exon:32615;Parent=Msa0582050-mRNA-1
chr4_1 exon 87710785 87711071 87710785 ID=Msa0582050-mRNA-1:exon:32616;Parent=Msa0582050-mRNA-1
chr4_1 exon 87712099 87712462 87712099 ID=Msa0582050-mRNA-1:exon:32617;Parent=Msa0582050-mRNA-1
chr4_1 five_prime_UTR 87710595 87710682 87710595 ID=Msa0582050-mRNA-1:five_prime_utr;Parent=Msa0582050-mRNA-1
chr4_1 five_prime_UTR 87710785 87710792 87710785 ID=Msa0582050-mRNA-1:five_prime_utr;Parent=Msa0582050-mRNA-1
chr4_1 CDS 87710793 87711071 87710793 ID=Msa0582050-mRNA-1:cds;Parent=Msa0582050-mRNA-1
chr4_1 three_prime_UTR 87712099 87712462 87712099 ID=Msa0582050-mRNA-1:three_prime_utr;Parent=Msa0582050-mRNA-1
Gene Sequence

>Msa0582050

ATGGCTTTCATAATGGAATTCGCTGAGAATCTGGTTCTGAAATTGATGGAAGATCCAAATGAAAGGGATCGTAGATTCAGGGAACATGTGTATAAGACGAAGGATAGATGCGCGAAGACGAAGGAGATGTGGAGTTACCCTATGCGACCTTATGGGTTCTGGACATTTGAACGCCATAACTCTCAGCTTGCTTGGGATGCTCAGATTAGTCAAGTTGCCGGTCGTAGAGATCCTTATGATGAAATCCTTTCACATTACCCTGCCTCGTCGTCGAAATGA
Protein sequence

>Msa0582050

MAFIMEFAENLVLKLMEDPNERDRRFREHVYKTKDRCAKTKEMWSYPMRPYGFWTFERHNSQLAWDAQISQVAGRRDPYDEILSHYPASSSK*