AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0683780


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0683780 MtrunA17_Chr4g0030851 100.000 55 0 0 1 55 1 55 3.19e-36 115
Msa0683780 MtrunA17_Chr2g0327811 89.091 55 6 0 1 55 1 55 7.42e-32 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0683780 AT3G46430.1 81.818 55 10 0 1 55 1 55 7.19e-30 99.4
Msa0683780 AT5G59613.1 81.818 55 10 0 1 55 1 55 7.19e-30 99.4
Msa0683780 AT5G59613.2 81.818 55 10 0 1 55 1 55 7.19e-30 99.4

Find 16 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCTCTCTTGAAGAAAAC+TGG 0.292105 4_4:-46697192 None:intergenic
TTTGTGATGCTGAACGAATT+TGG 0.293620 4_4:-46697414 None:intergenic
GGTAAGACCAAGAGAGAATT+TGG 0.344448 4_4:-46697268 None:intergenic
AAATTCTCTCTTGGTCTTAC+CGG 0.353041 4_4:+46697270 Msa0683780:CDS
CTTGTCACCAAATTCTCTCT+TGG 0.398915 4_4:+46697261 Msa0683780:CDS
CTCGTTGGATTCGCCGTCAC+CGG 0.441426 4_4:+46697234 Msa0683780:CDS
AGAGAGAATGGAAACGTACT+TGG 0.486234 4_4:+46697205 Msa0683780:CDS
CTCTTGAAGAAAACTGGCCA+TGG 0.493329 4_4:-46697186 None:intergenic
TCTTCGTCACACCAAAGTTA+CGG 0.513023 4_4:-46697079 None:intergenic
AATTCGTTCAGCATCACAAA+AGG 0.536789 4_4:+46697417 Msa0683780:CDS
ACGGCGAATCCAACGAGGAA+TGG 0.540309 4_4:-46697228 None:intergenic
GAATTTGGTGACAAGAGCTC+CGG 0.557010 4_4:-46697253 None:intergenic
CGTACTTGGCCATTCCTCGT+TGG 0.562709 4_4:+46697219 Msa0683780:CDS
AAATGAGACAGTTCGATCCA+TGG 0.569004 4_4:+46697169 Msa0683780:exon
GGTGACAAGAGCTCCGGTGA+CGG 0.581739 4_4:-46697247 None:intergenic
CGGTGACGGCGAATCCAACG+AGG 0.732353 4_4:-46697233 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCCTTTTTTATTGAACAATT+AGG + chr4_4:46697318-46697337 Msa0683780:intron 20.0%
!!! CCTTTTTTATTGAACAATTA+GGG + chr4_4:46697319-46697338 Msa0683780:intron 20.0%
!! TACGGTTTTTGTTACAAAAA+TGG - chr4_4:46697064-46697083 None:intergenic 25.0%
! AGAGAAATAGATTGAAATAC+CGG - chr4_4:46697292-46697311 None:intergenic 25.0%
! CCCTAATTGTTCAATAAAAA+AGG - chr4_4:46697322-46697341 None:intergenic 25.0%
! CTGCAACAAAAATAAAATCA+AGG - chr4_4:46697376-46697395 None:intergenic 25.0%
!!! TGATTTTATTTTTGTTGCAG+AGG + chr4_4:46697376-46697395 Msa0683780:intron 25.0%
CAAAAATGGTAGAGTTTAGA+TGG - chr4_4:46697050-46697069 None:intergenic 30.0%
TGTAACAAAAACCGTAACTT+TGG + chr4_4:46697068-46697087 Msa0683780:five_prime_UTR 30.0%
CATTCTCTCTTGAAGAAAAC+TGG - chr4_4:46697195-46697214 None:intergenic 35.0%
! CAGTTTTCTTCAAGAGAGAA+TGG + chr4_4:46697193-46697212 Msa0683780:CDS 35.0%
!! AAATTCTCTCTTGGTCTTAC+CGG + chr4_4:46697270-46697289 Msa0683780:CDS 35.0%
TTTGTGATGCTGAACGAATT+TGG - chr4_4:46697417-46697436 None:intergenic 35.0%
AATTCGTTCAGCATCACAAA+AGG + chr4_4:46697417-46697436 Msa0683780:CDS 35.0%
TCTTCGTCACACCAAAGTTA+CGG - chr4_4:46697082-46697101 None:intergenic 40.0%
AAATGAGACAGTTCGATCCA+TGG + chr4_4:46697169-46697188 Msa0683780:exon 40.0%
AGAGAGAATGGAAACGTACT+TGG + chr4_4:46697205-46697224 Msa0683780:CDS 40.0%
CTTGTCACCAAATTCTCTCT+TGG + chr4_4:46697261-46697280 Msa0683780:CDS 40.0%
GGTAAGACCAAGAGAGAATT+TGG - chr4_4:46697271-46697290 None:intergenic 40.0%
CTCTTGAAGAAAACTGGCCA+TGG - chr4_4:46697189-46697208 None:intergenic 45.0%
! GAATTTGGTGACAAGAGCTC+CGG - chr4_4:46697256-46697275 None:intergenic 45.0%
CGTACTTGGCCATTCCTCGT+TGG + chr4_4:46697219-46697238 Msa0683780:CDS 55.0%
ACGGCGAATCCAACGAGGAA+TGG - chr4_4:46697231-46697250 None:intergenic 55.0%
CTCGTTGGATTCGCCGTCAC+CGG + chr4_4:46697234-46697253 Msa0683780:CDS 60.0%
GGTGACAAGAGCTCCGGTGA+CGG - chr4_4:46697250-46697269 None:intergenic 60.0%
! CGGTGACGGCGAATCCAACG+AGG - chr4_4:46697236-46697255 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 46697035 46697442 46697035 ID=Msa0683780;Name=Msa0683780
chr4_4 mRNA 46697035 46697442 46697035 ID=Msa0683780-mRNA-1;Parent=Msa0683780;Name=Msa0683780-mRNA-1;_AED=0.28;_eAED=0.28;_QI=136|1|0.5|1|1|1|2|0|55
chr4_4 exon 46697035 46697291 46697035 ID=Msa0683780-mRNA-1:exon:11528;Parent=Msa0683780-mRNA-1
chr4_4 exon 46697396 46697442 46697396 ID=Msa0683780-mRNA-1:exon:11529;Parent=Msa0683780-mRNA-1
chr4_4 five_prime_UTR 46697035 46697170 46697035 ID=Msa0683780-mRNA-1:five_prime_utr;Parent=Msa0683780-mRNA-1
chr4_4 CDS 46697171 46697291 46697171 ID=Msa0683780-mRNA-1:cds;Parent=Msa0683780-mRNA-1
chr4_4 CDS 46697396 46697442 46697396 ID=Msa0683780-mRNA-1:cds;Parent=Msa0683780-mRNA-1
Gene Sequence

>Msa0683780

ATGAGACAGTTCGATCCATGGCCAGTTTTCTTCAAGAGAGAATGGAAACGTACTTGGCCATTCCTCGTTGGATTCGCCGTCACCGGAGCTCTTGTCACCAAATTCTCTCTTGGTCTTACCGAGGAAGATGCTAAGAATTCCAAATTCGTTCAGCATCACAAAAGGTAA

Protein sequence

>Msa0683780

MRQFDPWPVFFKREWKRTWPFLVGFAVTGALVTKFSLGLTEEDAKNSKFVQHHKR*